| 摘要: |
| ［摘要］ 目的 探索长链非编码RNA-H19对急性髓系白血病细胞增殖和细胞凋亡的调控作用。方法 采用RAN干扰技术对急性髓系白血病细胞系HL-60、THP-1和U937的LncRNA-H19进行表达沉默，用实时荧光定量PCR（qPCR)检测LncRNA-H19在细胞中的表达情况。用MTT检测细胞增殖情况，用流式细胞术检测细胞凋亡情况。结果 三种白血病细胞系分别被3条si-RNA干扰后，LncRNA-H19表达均明显下调。MTT结果显示，RNA干扰后，LncRNA-H19干扰细胞组的细胞增殖明显低于阴性对照组,而流式细胞术显示，si-H19干扰细胞组的细胞凋亡明显高于阴性对照组，差异有统计学意义（P<0.05）。结论 急性髓系白血病细胞中，LncRNA-H19具有调控急性髓系白血病细胞增殖和细胞凋亡的功能。 |
| 关键词: 长链非编码RNA-H19 急性白血病 细胞增殖 细胞凋亡 |
| DOI：10.3969/j.issn.1674-3806.2017.12.01 |
| 分类号:R 733.7 |
| 基金项目:国家自然科学基金资助项目（编号：81500126、81473001）；广东省自然科学基金资助项目(编号：2015A030313727)；广东省科技项目（编号：2013B021800065） |
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| LncRNA-H19 regulates cell proliferation and apoptosis in acute myeloid leukemia |
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WANG Cai-xia, ZHOU Zhi-heng, CHEN Bao-xin, et al.
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Department of Hematology, the First People′s Hospital of Guangzhou City, Guangdong 510180, China
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| Abstract: |
| ［Abstract］ Objective To explore the regulating effect of long non-coding RNA-H19(LncRNA-H19) on cell proliferation and apoptosis in different acute myeloid leukemia cell lines.Methods HL-60, THP-1 and U937 cells were transfected with si-H19 and si-NC, respectively. MTT assay was performed to determine the proliferation, and cell apoptosis was assayed by flow cytometry using Annexin V-FITC/PI.Results The expression of LncRNA-H19 in AML cells was significantly down-regulated after the treatment of si-H19 for 72 hours. Down-regulation of LncRNA-H19 in AML cell lines HL-60, THP-1 and U937 inhibited cell proliferation, and induced cell apoptosis(P<0.01).Conclusion LncRNA-H19 can regulate cell proliferation and apoptosis in acute myeloid leukemia cell lines. |
| Key words: LncRNA-H19 Acute myeloid leukemia Cell proliferation Apoptosis |
