| 摘要: |
| ［摘要］ 目的 探讨以预糊化淀粉为支架的新细胞块免疫组化染色技术在浆膜腔积液细胞诊断中的应用价值。方法 收集该院2015-01～2016-09接收的浆膜腔积液标本226例，分别做传统细胞涂片和以预糊化淀粉为支架制作细胞块，根据诊断需要选做免疫组化染色。结果 传统细胞涂片阳性检出率为25/226(11.06%)，细胞块结合免疫组化阳性检出率为48/226（21.24%），明显优于传统细胞涂片(P<0.05)。结论 以预糊化淀粉为支架的新细胞块技术操作简单，切片质量优良，结合免疫组化染色明显提高了浆膜腔积液恶性肿瘤细胞的检出率，并有助于确定转移性肿瘤的组织来源。 |
| 关键词: 预糊化淀粉 细胞块 免疫组化 浆膜腔积液 |
| DOI：10.3969/j.issn.1674-3806.2018.07.18 |
| 分类号:R 361+.3 |
| 基金项目: |
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| Application of cell block combined with immunohistochemistry in cytodiagnosis of dropsy of serous cavity |
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CHEN Ji-qiong, HUANG Jia-qin, CAI Shu-ying, et al.
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Department of Pathology, Zhangmutou Hospital of Dongguan City, Guangdong 523633, China
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| Abstract: |
| ［Abstract］ Objective To investigate the application value of a new cell block technique based on pre-gelatinized starch combined with immunohistochemistry in cytodiagnosis of dropsy of serous cavity. Methods 226 specimens of dropsy of serous cavity were collected. Traditional cell smears and cell blocks based on pre-gelatinized starch were made, and immunohistochemical staining was performed according to the diagnosis. Results The positive rate of the traditional cell smears(11.06%) was significantly lower than that of the cell block combined with immunohistochemistry(21.24%)(P<0.05). Conclusion The new cell block technique based on pre-gelatinized starch is simple in operation, good in slicing quality and good in immunohistochemical staining. Cell block combined with immunohistochemistry can improve the detection rate of malignant tumor cells and determine the source of metastatic tumors in dropsy of serous cavity. |
| Key words: Pre-gelatinized starch Cell block Immunohistochemistry Dropsy of serous cavity |
