| 摘要: |
| ［摘要］ 目的 观察Toll样受体4、环氧化酶Ⅱ在兔动脉粥样硬化模型中的表达水平，探讨两者在动脉粥样硬化下协同表达情况和阿托伐他汀治疗对表达的影响。方法 将36只体质量范围在1.8～2.4 kg的3月龄健康雄性新西兰实验兔随机分为对照组、模型组、治疗组三组，每组12只。予高脂饲料对模型组和治疗组两组造模8周，治疗组在造模后以阿托伐他汀2 mg(/kg·d)干预8周。监测实验进程各组0周、8周、16周外周血脂水平变化，对比各组16周冠状动脉组织切片、Toll样受体4和环氧化酶ⅡmRNA表达、不良事件发生等情况。结果 与对照组比较，模型组血总胆固醇、甘油三酯、低密度脂蛋白胆固醇水平升高，Toll样受体4和环氧化酶Ⅱ表达增强（P均<0.01）；治疗组经阿托伐他汀治疗后，血液中总胆固醇、甘油三酯、低密度脂蛋白胆固醇水平下降和高密度脂蛋白胆固醇水平上升（P均<0.05），Toll样受体4、环氧化酶Ⅱ表达下调（P均<0.01）；经对模型组和治疗组动脉粥样硬化组织光镜检查结果进行分级，动脉粥样硬化模型组最严重，治疗组其次，与对照组比较差异有统计学意义（P<0.01）。结论 动脉粥样硬化中Toll样受体4、环氧化酶Ⅱ表达协同升高可能是其发病机制之一，阿托伐他汀可有效降低两项指标在模型体内表达水平。 |
| 关键词: 动脉粥样硬化 Toll样受体4 环氧化酶Ⅱ 阿托伐他汀 |
| DOI：10.3969/j.issn.1674-3806.2017.01.02 |
| 分类号:R 54 |
| 基金项目:广东省中医药局科研课题（编号：20151114） |
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| Effects of atorvastatin on the expressions of TLR4 and COX-2 in atherosclerotic rabbits |
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LI Mao-qing, XU Bi-hong, PENG Zhi-yong, et al.
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Department of Rehabilitation, Rehabilitation Hospital of Meizhou City, Guangdong 514000, China
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| Abstract: |
| ［Abstract］ Objective To observe the expression levels of Toll-like receptors 4(TLR4) and cyclooxygenase-2(Cox-2) in atherosclerotic rabbits and to explore the effects of atorvastatin on the expressions of TLR4 and COX-2.Methods A total of 36 New Zealand white rabbits (1.8～2.4 kg) were randomly divided into the normal group(n=12), the model group(n=12) and the treatment group(n=12). Except for the normal group, the other groups were fed with high-fat diet for 8 weeks to establish the atherosclerotic model. After the model was successfully established, the treatment group was fed with atorvastatin 2 mg/(kg·d) for 8 weeks. The levels of peripheral blood lipid were monitored at the time points of 0, 8 and 16 weeks after the treatment. The expressions of TLR4 and COX-2 and the adverse events were compared among the three groups 16 weeks after the treatment.Results Compared with those in the control group, the levels of serum TC, TG and LDL, and the expressions of TLR4 and COX-2 were significantly elevated in the model group(P<0.01). After the treatment of atorvastatin, the levels of serum TC, TG and LDL were decreased and the HDL level was increased(P<0.05), and the expressions of TLR4 and COX-2 were reduced significiantly in the treatment group(P<0.01). The grading of atherosclerosis under light microscopy was the most severe in the model group, followed by the treatment group, with significant differences between the two groups and the control group(P<0.01).Conclusion The co-expression of TLR4 and COX-2 may play an important role in the pathogenesis of atherosclerosis. Atorvastatin can effectively reduce the expression levels of TLR4 and COX-2 of atherosclerosis in the rabbit model. |
| Key words: Atherosclerosis Toll-like receptor 4 Cyclooxygenase Ⅱ Atorvastatin |
