| 摘要: |
| ［摘要］ 目的 了解毛细管电泳（CE）与高效液相色谱法（HPLC）在Hb Q-Thailand检测中的差异。方法 收集10例CE筛查出的可疑Hb Q-Thailand，经地贫基因分析和DNA测序确诊后，再用HPLC检测比对。结果 10例样本中CE均检出Hb Q-Thailand和Hb Q-Thailand A2（Hb QA2），而HPLC仅检出10例Hb Q-Thailand和5例Hb QA2，而且HPLC错误检出Hb Q-H病1例存在Hb A2。CE和HPLC检出Hb Q-Thailand、Hb A2、Hb QA2的含量分别为（34.41±21.10）%、（1.68±0.57）%、（0.77±0.10）%和（31.72±21.46）%、（2.43±0.22）%、（0.24±0.24）%，两组Hb Q-Thailand差异无统计学意义（P>0.05），Hb A2和Hb QA2的差异有统计学意义（P<0.01）。结论 CE在检测Hb Q-Thailand中优于HPLC。 |
| 关键词: Hb Q-Thailand 毛细管电泳 高效液相色谱法 异常血红蛋白 |
| DOI：10.3969/j.issn.1674-3806.2017.01.07 |
| 分类号:R 446 |
| 基金项目:广西卫计委科研课题（编号：Z2011449） |
|
| Comparison of capillary electrophoresis and high performance liquid chromatography for detection of hemoglobin Q-Thailand |
|
WEI Jin-hua, LI You-qiong, TAN Chun-yan, et al.
|
|
Department of Clinical Laboratory, Long An Maternity and Child Healthcare Hospital of Guangxi Zhuang Autonomous Region, Nanning 532700, China
|
| Abstract: |
| ［Abstract］ Objective To study the difference in detection of Hb Q-Thailand between capillary electrophoresis(CE) and high performance liquid chromatography(HPLC).Methods Ten samples of suspicious Hb Q-Thailand by CE were confirmed with thalassemia gene analysis and DNA sequencing and their results were compared with those detected by HPLC.Results Ten samples with Hb Q-Thailand and Hb Q-Thailand A2(Hb QA2) were all detected by CE, but Hb Q-Thailand and Hb QA2 were detected respectively in 10 samples and 5 samples by HPLC. Moreover, Hb A2 was wrongly detected in Hb Q-H disease by HPLC. The values of Hb Q-Thailand, Hb A2 and Hb QA2 detected by CE and HPLC were （34.41±21.10）%,（1.68±0.57）%,（0.77±0.10）% and（31.72±21.46）%,（2.43±0.22）%,（0.24±0.24）% respectively. There were significant differences in the detection of Hb A2 and Hb QA2 between the different methods of CE and HPLC（P<0.01）, but there was no significant difference in the detection of Hb Q-Thailand between the two methods（P>0.05）.Conclusion CE is better in detection of Hb Q-Thailand than HPLC. |
| Key words: Hb Q-Thailand Capillary electrophoresis (CE) High performance liquid chromatography(HPLC) Abnormal hemoglobin |
