| 摘要: |
| ［摘要］ 目的 探讨P16、P53、Beclin-1三种蛋白在宫颈鳞癌组织及宫颈正常组织中的表达及临床病理意义。方法 应用免疫组化SP法检测宫颈正常组织组40例及宫颈鳞癌组织组43例P16、P53和Beclin-1蛋白表达水平。结果 宫颈鳞癌组织中P16及P53表达阳性率分别为100.0%和86.0%，高于宫颈正常组织的5.0%和5.0%(P均<0.05)，其表达强度与宫颈鳞癌组织呈正相关。而Beclin-1在宫颈鳞癌组织中表达阳性率为55.8%，正常宫颈组织为85.0%(P<0.01)，表达强度与宫颈鳞癌组织呈负相关。结论 P16、P53蛋白及Beclin-1蛋白与宫颈鳞癌的发生发展相关，P16和P53的表达随宫颈上皮恶变的进展而增加，而Beclin-1在宫颈恶性病变中的表达明显下调。 |
| 关键词: 宫颈癌 宫颈正常组织 P16 P53 Beclin-1 免疫组化 |
| DOI：10.3969/j.issn.1674-3806.2017.02.05 |
| 分类号:R 737.33 |
| 基金项目:广东清远市科技计划项目（编号：2015B044） |
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| Expressions of P16, P53 and Beclin-1 in cervical squamous cell carcinoma and normal cervical tissues |
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ZHANG Xin-ning, WU Hai-yan, LIU Yong-zhu
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Department of Gynaecology, the People′s Hospital of Qingyuan City, Guangdong 511500, China
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| Abstract: |
| ［Abstract］ Objective To investigate the expressions of P16, P53 and Beclin-1 in cervical squamous cell carcinoma(CSCC) and normal human cervical tissues and their ralationships with clinicopathologica parameters.Methods The expressions of P16, P53 and Beclin-1 protein were detected by immunohistochemical(SP method) in 40 cases of normal human cervical tissues and 43 of CSCC.Results The expression rates of P16 and P53 in the CSCC were higher than those in the normal cervical tissues(100.0% vs 5.0% and 86.0% vs 5.0%)(P<0.05), and the expression intensity was positively correlated with the level of cervical squamous cell carcinoma. The expression of Beclin-1 in the CSCC(55.8%) was lower than that in the normal cervical tissues(85.0%)(P<0.01), and the expression intensity was negatively correlated with cervical squamous cell carcinoma.Conclusion The expression levels of P16, P53 and Beclin-1 protein are closely related to the development of CSCC. The expressions of P16 and P53 protein are increased with the progression of cervical epithelial malignant, and the expression of Beclin-1 is down-regulated in malignant cervical lesions. |
| Key words: Cervical squamous cell carcinoma Cervical normal tissue P16 P53 Beclin-1 Immunohistochemistry |
