| 摘要: |
| ［摘要］ 目的 探讨血管内皮生长因子（vascular endothelial growth factor,VEGF）通路对体外冲击波联合仙桃草治疗兔桡骨骨不连骨痂中成骨细胞增殖作用的影响。方法 制作100只兔桡骨骨不连模型，造模成功后随机分为四组：A组为体外冲击波联合仙桃草治疗组，B组为单纯仙桃草治疗组，C组为单纯体外冲击波治疗组，D组为完全对照组。于治疗后不同时相点（第1、2、4、8、12周）分别取材，H-E染色观察成骨细胞计数,免疫组织化学观察VEGF表达情况。结果 体外冲击波联合仙桃草治疗组能够加速成骨细胞增殖并上调VEGF表达，与其他三组比较差异有统计学意义（P<0.01）。其中，成骨细胞增殖与VEGF表达的变化曲线大致相同，于第2周后明显增多，第8周到达峰值，第12周时较前稍降低，但数量仍较多。结论 体外冲击波联合仙桃草治疗骨不连效果安全可靠，其机制可能是通过对骨痂中VEGF细胞因子的影响，促进成骨细胞的增殖，来达到治疗骨不连的目的。 |
| 关键词: 体外冲击波 仙桃草 骨不连 成骨细胞 血管内皮生长因子 |
| DOI：10.3969/j.issn.1674-3806.2017.03.03 |
| 分类号:R 68 |
| 基金项目:广西医疗卫生重点科研课题（编号：重2011120）；广西卫计委中医药科技专项面上课题（编号：GZPT1242）；广西适宜卫生技术研发推广人才小高地项目（编号：006011） |
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| Effects of VEGF on the proliferations of osteoblast in callus in the treatment of fracture nonunion by extracorpoporeal shock wave combined with veronica peregrina |
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ZHAO Zi-xing, LI Hong-yu, XI Li-cheng, et al.
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Guangxi University of Traditional Chinese Medicine, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To study effects of vascular endothelial growth factor(VEGF) on the proliferations of osteoblast in callus in the treatment of fracture nonunion by extracorpoporeal shock wave combined with veronica peregrina.Methods One hundred New Zealand white rabbits were selected to make a modle of bone nonunion of radius. They were randomly divided into four groups. Group A was treated by extracorporeal shock wave combine with veronica peregrina. Group B was treated by extracorporeal shock wave. Group C was treated with veronica peregrina and group D was taken as the control group. The samples in each group were collected at the end of 1, 2, 4, 8 and 12 weeks respectively after the therapies, and hematoxylin-eosin stain was used to assess the osteoblast counts. VEGF expression was assessed by immunohistochemistry.Results Compared with those in the other three groups, the proliferation of osteoblast was significantly increased and the expression of VEGF was up-regulated in group A（P<0.01）. The osteoblast counts and the expression of VEGF shared a similar changing trend. The osteoblast counts in each group started to increase obviously at 2 weeks and reached a peak at 8 weeks, and then went down at 12 weeks and persisted on a high level.Conclusion Extracorporeal shock wave combine with veronica peregrina is safe and effective to treat fracture nonunion. The mechanism involves up-regulating the expression of VEGF and inducing the proliferation of osteoblast. |
| Key words: Extracorporeal shock wave Veronica peregrina Fracture nonunion Osteoblast Vascular endothelial growth factor(VEGF) |
