| 摘要: |
| ［摘要］ 目的 探讨龙血竭总黄酮对心肌缺血再灌注损伤大鼠心肌细胞的保护作用。方法 将40只SD大鼠随机分为5组，空白对照组（Control组）、假手术组（Sham组）、心肌缺血再灌注损伤组（MIRI组）、假手术+SDF组（SDF组）、MIRI+SDF组（MIRI&SDF组），每组8只。采用结扎左冠状动脉前降支30 min再灌注2 h的方法建立大鼠MIRI模型，于术前1周开始灌药。采用比色法测定血清肌酸激酶（CK）、肌酸激酶同工酶（CK-MB）和乳酸脱氢酶（LDH）水平，采用TTC染色评估心肌坏死面积，用TUNEL法检测心肌细胞凋亡率。结果 与Control、Sham和SDF组比较，MIRI组、MIRI&SDF组的CK、LDH、CK-MB的水平均明显升高（P<0.05）。与MIRI组比较，MIRI&SDF组的CK、LDH、CK-MB的水平明显下降（P<0.05）。MIRI&SDF组心肌梗死面积和心肌细胞的凋亡率明显小于MIRI组（P<0.05）。结论 龙血竭总黄酮对MIRI大鼠心肌细胞具有较好的保护作用。 |
| 关键词: 龙血竭总黄酮 心肌缺血再灌注损伤 细胞凋亡 |
| DOI：10.3969/j.issn.1674-3806.2017.09.08 |
| 分类号:R 542.2 |
| 基金项目:广西自然科学基金项目（编号：2010GXNSFA013269）；广西中医药管理局科研项目(编号：GZZC14-63) |
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| Study on protective effect of Sanguis Draconis flavones on myocardial cells in rats with myocardial ischemia-reperfusion injury |
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LIANG Li-mei, FENG Xiang-ling, LIU Yan, et al.
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Xiangya School of Public Health, Central South University, Changsha 410008, China
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| Abstract: |
| ［Abstract］ Objective To investigate the protective effect of Sanguis Draconis flavones（SDF）on myocardial cells in rats with myocardial ischemia-reperfusion injury(MIRI).Methods 40 SD rats were randomly divided into five groups: control group, sham group, MIRI group, sham+SDF group and MIRI+SDF group, with 8 cases in each group. The MIRI model was established by ligating left anterior descending coronary artery for 30 minutes and reperfusing for 2 hours. The drugs were administered one week before operation. The levels of serum creatine kinase(CK), creatine kinase isoenzyme MB(CK-MB) and lactic dehydrogenase(LDH) were measured by colorimetric assay. The area of myocardial necrosis was assessed by triphenyltetrazolium chloride(TTC) staining, and the apoptosis rate of cardiomyocytes was detected by terminal-deoxynucleoitidyl transferase mediated nick end labeling(TUNEL).Results Compared with those in the control group, sham group and the sham+SDF group, the levels of CK, LDH and CK-MB in the MIRI group and the MIRI+SDF group were significantly higher(P<0.05), while compared with those in the MIRI group, the levels of CK, LDH and CK-MB in the MIRI+SDF group were significantly lower(P<0.05). The area of myocardial infarction in the MIRI+SDF group was significantly smaller than that in the MIRI group(P<0.05), and the apoptotic rate of myocardial cells in the MIRI+SDF group was significantly smaller than that in the MIRI group(P<0.05).Conclusion Sanguis Draconis flavones can effectively protect the myocardial cells of rats with myocardial ischemia-reperfusion injury. |
| Key words: Sanguis Draconis flavones（SDF） Myocardial ischemia-reperfusion injury(MIRI) Cell apoptosis |
