| 摘要: |
| ［摘要］ 目的 检测miR-155-5p在鼻咽癌组织中的表达水平，探讨miR-155-5p与远处转移及临床预后的关系。方法 收集有远处转移（转移组）和仅有原发病灶（非转移组）鼻咽癌活检标本共60例，应用实时荧光定量-PCR（QRT-PCR）方法检测鼻咽癌中miR-155-5p的表达水平，分析miR-155-5p与临床病理参数及预后的关系。结果 远处转移组miR-155-5p表达水平较非转移组显著上调，相对表达量为［（4.92±5.52） vs （1.98±2.66）］（t=-2.505，P=0.017）。miR-155-5p表达水平与性别、年龄、发病部位、淋巴结转移、T分期及临床分期等均无明显的相关关系（P>0.05）。以miR-155-5p相对表达量中位值2-△△Ct=1.66为截断值，Kaplan-Meier生存曲线和Log-rank检验结果显示，miR-155-5p高表达组鼻咽癌患者5年总生存率明显低于低表达组（χ2=4.18，P=0.041）。结论 miR-155-5p表达水平与鼻咽癌远处转移密切相关，可能成为辅助判断患者预后的生物学指标。 |
| 关键词: 鼻咽癌 miR-155-5p 实时荧光定量-PCR 转移 |
| DOI：10.3969/j.issn.1674-3806.2018.09.01 |
| 分类号:R 739.62 |
| 基金项目:国家自然科学基金资助项目（编号：81360355）；广西卫计委科研课题（编号：Z2013355，Z2014622，Z2016590） |
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| Correlation of miR-155-5p with distant metastasis and prognosis in nasopharygeal carcinoma |
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HUANG Yong-ta, YE Qiu-rong, WENG Jing-jin, et al.
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Department of Pathology, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To investigate the expression of miR-155-5p in nasopharyngeal carcinomas（NPC） and its relationship between metastasis and prognosis in NPC patients. Methods The biopsy specimens of 60 NPC patients with distant metastasis(metastatic group) or primary cancer focus alone(non-metastatic group) in our hospital were collected, and the expressions of miR-155-5p in the specimens were detected by real time fluorescent quantitative PCR(QRT-PCR). The relationship between miR-155-5p and the clinicopathological parameters and prognosis were analyzed. Results Compared with that in the non-metastatic group,the level of miR-155-5p in the metastatic group was significantly up-regulated［（4.92±5.52） vs （1.98±2.66）］（t=-2.505,P=0.017）. No significant differences were observed among miR-155-5p and sex,age,location, lymph node metastasis, T grades and clinical stages(P>0.05). If the median level of miR-155-5p was considered as cut-off value, Kaplan - Meier survival curve and the Log-rank test results showed that the overall 5 year survival rate of the NPC patients with low expression of miR-155-5p was significantly higher than that of the NPC patients with high expression of miR-155-5p(χ2=4.18, P=0.041 ). Conclusion The expression level of miR-155-5p may play a key role in metastasis of NPC. It may be a novel biomarker in predicting prognosis of NPC. |
| Key words: Nasopharyngeal carcinomas（NPC） miR-155-5p Real time fluorescent quantitative PCR(QRT-PCR) Metastasis |
