| 引用本文: | 杨剑榆,黄晓雷,李保珍,李戈辉,徐阳,李元涛.白藜芦醇通过抑制NF-κB活性减轻大鼠肠缺血再灌注继发性肺损伤[J].中国临床新医学,0,():-. |
| yangjianyu,huangxiaolei,libaozhen,ligehui,xuyang,liyuantao.白藜芦醇通过抑制NF-κB活性减轻大鼠肠缺血再灌注继发性肺损伤[J].中国临床新医学,0,():-. |
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| 摘要: |
| 目的 评价白藜芦醇(Resveratrol, Res)预处理对大鼠肠缺血再灌注继发肺损伤时NF-κB活性的影响。方法 健康清洁级雌性SD大鼠24只,体重180~200 g,采用随机数字表法分为3组(n=8)：假手术组(S组)、肠缺血再灌注组(M组)和白藜芦醇预处理组(R组)。采用夹闭肠系膜上动脉75 min后再灌注4h的方法制备肠缺血再灌注继发性肺损伤(IIR-ALI)模型。于模型制备前5 天,R组每天腹腔注射Res 15mg/kg(采用生理盐水稀释),S组和M组腹腔注射等容量生理盐水。于再灌注4 h时放血处死大鼠,收集肺组织和支气管肺泡灌洗液标本,测肺湿干重比值(W/D)、支气管肺泡灌洗液蛋白浓度、支气管肺泡灌洗液中IL-1β和TNF-α含量以及光镜下行肺损伤评分(Hofbauer评分)；采用免疫印迹检测肺组织核蛋白NF-κB p65的表达。结果 与S组比较,M组和R组大鼠肺组织损伤严重,肺组织损伤评分、肺W/D值、支气管肺泡灌洗液蛋白浓度、支气管肺泡灌洗液蛋白浓度IL-1β和TNF-α含量以及肺组织核蛋白NF-κB p65的表达增加(P<0.05)；与M组比较,R组大鼠肺组织损伤减轻,肺组织损伤评分、肺W/D值、支气管肺泡灌洗液蛋白浓度、支气管肺泡灌洗液蛋白浓度IL-1β和TNF-α含量以及肺组织核蛋白NF-κB p65的表达下降(P<0.05)。结论 白藜芦醇预处理减轻大鼠肠缺血再灌注继发性肺损伤,机制可能与其抑制肺组织NF-κB活性有关。 |
| 关键词: 白藜芦醇 再灌注损伤 急性肺损伤 NF-κB 细胞因子 |
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| Resveratrol preconditioning attenuated acute lung injury induced by intestinal ischemia-reperfusion in rats by inhibiting NF-kappaB activity |
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yangjianyu, huangxiaolei, libaozhen, ligehui, xuyang, liyuantao
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SHENZHEN MATERNITY AND CHILD HEALTHCARE HOSPITAL
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| Abstract: |
| Objective To evaluate the effect of resveratrol preconditioning on NF-kappaB activity during acute lung injury induced by intestinal ischemia-reperfusion(IIR) in rats. Methods Twenty-four healthy female SD rats were randomly divided into three groups(n=8): Sham-operated group(S group), Intestinal ischemia-reperfusion mode group (M group), Resveratrol pretreatment group(R group). Acute lung injury induced by intestinal ischemia-reperfusion(IIR-ALI) in rats was conducted by clamping the superior mesenteric artery (SMA) for 75 minutes followed by 4h reperfusion. In the R group, rats were treated with resveratrol (15mg/kg, intraperitoneally) for 5 days before IIR. In other groups, rats were treated with the same volume of NS for 5 days before operation. All rats were sacrificed after 4 hours’ reperfusion of SMA. The lung tissues were obtained for wet/dry weight ratio(W/D) and pathological investigation (Hofbauer’s scoring system) and the protein expression of nucleoprotein NF-kappaB p65 while Bronchoalveolar Lavage Fluid was collected to detect the total proteins content and the levels of TNF-α and IL-1β．Results Comparing with S group, the Hofbauer’s scoring, W/D, NF-kappaB p65 expression and BALF indexes including the total proteins content and the levels of TNF-α and IL-1β increased significantly (P<0.05) in M group and R group. Comparing with M group, the Hofbauer’s scoring, W/D, NF-kappaB p65 expression of lung tissue and BALF indexes decreased obviously in R group (P<0.05). Conclusion Resveratrol preconditioning could attenuate the acute lung injury induced by intestinal ischemia-reperfusion in rats. This effect may be related to inhibiting NF-kappaB activity in lung. |
| Key words: Resveratrol Reperfusion Injury acute lung injury NF-kappaB cytokine |
