引用本文:梁丽梅,冯湘玲,刘 燕,程初勇,黄照河.龙血竭总黄酮对心肌缺血再灌注损伤大鼠心肌细胞的保护作用研究[J].中国临床新医学,0,():-.
LIANG Limei,FENG Xiangling,LIU Yan,CHENG Chuyong,HUANG Zhaohe.龙血竭总黄酮对心肌缺血再灌注损伤大鼠心肌细胞的保护作用研究[J].中国临床新医学,0,():-.
【打印本页】   【下载PDF全文】   【查看/发表评论】  【EndNote】   【RefMan】   【BibTex】
过刊浏览    高级检索
本文已被：浏览 148次   下载 0次  
分享到： 微信 更多
字体:加大+|默认|缩小-
龙血竭总黄酮对心肌缺血再灌注损伤大鼠心肌细胞的保护作用研究
梁丽梅1, 冯湘玲1, 刘 燕2, 程初勇2, 黄照河2
1.中南大学湘雅公共卫生学院 湖南长沙;2.右江民族医学院附属医院 广西百色
摘要:
【】目的 探讨龙血竭总黄酮对心肌缺血再灌注损伤大鼠心肌细胞的保护作用。方法 将40只SD大鼠随机分为5组,空白对照组(Control组)、假手术组(Sham组)、MIRI组、假手术组+SDF组(SDF组)、MIRI组+SDF组(MIRI SDF组),每组8只。采用结扎左冠状动脉前降支30分钟再灌注2小时的方法建立大鼠MIRI模型,于术前1周开始灌药。采用比色法测定血清肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)和乳酸脱氢酶(LDH)水平,采用TTC染色评估心肌坏死面积,用TUNEL法检测心肌细胞凋亡率。结果 与Control、Sham和SDF组比较,MIRI组、MIRI SDF组的CK、LDH、CK-MB的水平均明显升高(p<0.05),而与MIRI组比较,MIRI SDF组的CK、LDH、CK-MB的水平明显下降(p<0.05)。MIRI SDF组心肌梗死面积明显小于MIRI组(p<0.05),MIRI SDF组心肌细胞的凋亡率也明显小于MIRI组(p<0.05),其差异均具有统计学意义。结论 龙血竭总黄酮对心肌缺血再灌注损伤大鼠心肌细胞具有较好的保护作用。
关键词:  龙血竭总黄酮  心肌缺血再灌注损伤  细胞凋亡
DOI：
分类号:
基金项目:广西自然科学(项目合同号：2010GXNSFA013269)；广西中医药管理局科研项目(项目编号：GZZC14-63)
Study on protective effect of ] total flavonoids of dragon
LIANG Limei,FENG Xiangling,LIU Yan,CHENG Chuyong,HUANG Zhaohe
Xiangya School of Public Health,Central South University
Abstract:
Objective To investigate the protective effect of total flavonoids of dragon"s blood on myocardial cells in rats with myocardial ischemia-reperfusion injury(MIRI).Methods 40 SD rats were randomly divided into 5 groups: control group, sham group, MIRI group, sham SDF group and MIRI SDF group, with 8 rats in each group. MIRI model was established by ligating left anterior descending coronary artery for 30 minutes and reperfusing for 2 hours. The drugs were administered at 1 week before operation. Serum creatine kinase(CK), creatine kinase isoenzyme MB(CK-MB) and lactic dehydrogenase (LDH) levels were measured by colorimetric assay. The area of myocardial necrosis was assessed by TTC staining, and the apoptosis rate of myocardium was detected by TUNEL. Results Compared with the control group and the sham SDF group, the levels of CK, LDH and CK-MB in the MIRI group and the MIRI SDF group were significantly higher (P<0.05), while compared with the MIRI group, the levels of CK, LDH and CK-MB in the MIRI SDF group were significantly lower(P<0.05). The myocardial infarction area of the MIRI SDF group was significantly smaller than that of the MIRI group(P<0.05), and the apoptotic rate of myocardial cells in the MIRI SDF group was significantly smaller than that in the MIRI group(P<0.05), so the difference was statistically significant. Conclusion The total flavone of dragon"s blood has a good protective effect on myocardial cells of rats with MIRI.
Key words:  total flavone of dragon