| 引用本文: | 方盼盼,王颍源,杨俊文,高凯杰.基质辅助激光解吸电离飞行时间质谱技术在直接鉴定血流感染病原菌中的应用[J].中国临床新医学,0,():-. |
| 方盼盼.基质辅助激光解吸电离飞行时间质谱技术在直接鉴定血流感染病原菌中的应用[J].中国临床新医学,0,():-. |
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| 摘要: |
| [摘 要]目的 探讨利用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS)技术建立血流感染病原菌快速、准确的鉴定方法。方法 收集郑州大学附属儿童医院检验科2017-07~2017-12血培养仪阳性报警血培养瓶524个，抽吸血培养瓶中血液至血清分离胶管，采用差速离心法富集细菌，利用MALDI-TOF MS对富集后的菌液进行快速鉴定，并与传统培养2448ｈ后形成的菌落最终鉴定结果进行比较。结果 阳性报警血培养瓶524个，496个为单菌株血流感染，16个为混合菌感染，12例血培养报警假阳性结果。在496例单菌株所致血流感染中，菌株的属、种鉴定符合率分别为98.6%（489／496）和95.8%（475／496）。基质辅助激光解吸电离飞行时间质谱（Matrixassis)其中，120株革兰阴性菌属、种鉴定符合率分别为99.2%（119／120）和98.3%（118／120）；376株革兰阳性菌属、种鉴定符合率分别为98.4%（370／376）和94.9%（357／376）。16例混合菌感染及12个血培养报警假阳性均未得到鉴定结果。结论 本研究利用MALDI-TOF MS对差速离心富集后的菌液进行直接鉴定，与传统的培养鉴定方法相比，对血流感染中主要病原菌的鉴定符合率较高，且方法快速、简便，成本低廉，有效缩短了检测时间，而且鉴定准确率高，适合在临床微生物实验室中推广应用。 |
| 关键词: 基质辅助激光解吸电离飞行时间质谱 血流感染 细菌鉴定 |
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| Application of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry for Direct Identification of Pathogenic Bacteria in Bloodstream Infection |
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方盼盼1,2,3
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1.Zhengzhou Children'2.'3.s Hospital
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| Abstract: |
| [Abstract] Objective To investigate a rapid and accurate method for the identification of bloodstream infection pathogens by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Methods A total of 524 positive blood culture bottles for blood culture instruments were collected from the Department of Clinical Laboratory of Children's Hospital Affiliated to Zhengzhou University from July 2017 to December 2017. Blood was drawn from blood culture bottles to serum separation tubes, and bacteria were collected using differential centrifugation. MALDI- was used to collect bacteria. The TOF MS was used to rapidly identify the enriched bacteria solution and compare it with the final identification result of colonies formed after 2448 hours of traditional culture. RESULTS: Of the 524 positive blood culture samples, 496 were single strain bloodstream infections, 16 were mixed bacteria infections and 12 were blood cultures False positive result of the alarm. Among the 496 single-strain bloodstream infections, the genus and species identification rates of the strains were 98.6% (489/496) and 95.8% (475/496). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (Matrixassis), among which 120 strains of Gram-negative bacteria, the species identification rate was 99.2% (one hundred and nine hundred and ninety-nine) and 98.3% (one hundred and twenty) 176 parts of Gram-positive genus, the species identification rate was 98.4% (370/376) and 94.9% (357/376). 16 cases of mixed bacteria infection and 12 blood cultures None of the false positives of the alarms were identified. Conclusion This study used MALDI-TOF MS to directly identify the bacterial liquid after differential centrifugation. Compared with the traditional culture identification method, the identification rate of the main pathogens in bloodstream infection is high, and the method is fast and simple. The cost is low, the detection time is effectively shortened, and the identification accuracy is high, which is suitable for popularization and application in a clinical microbiology laboratory. |
| Key words: matrix-assisted laser desorption ionization time-of-flight mass spectrometry bloodstream infection bacterial identification |
