［Abstract］　Objective　To investigate the experiment condition of rabbit diabetes model produced by alloxan and evaluate stability of the model.Methods　Impacts of fasting time, alloxan route of administration, dose, and different handling ways of after administration on the model success rate, mortality, blood glucose were studyed in 48 rabbits. Results　Fasting time, administration route, dose and the treatment after administration were the main conditions which affect the molde. Conclusion　By selecting healthy rabbit after fasting 6 hours and injecting 130 mg/kg of alloxan from ear vein and immediately feed after injection, the diabetics rabbit model which has hight success rate, low mortality and stable blood sugar can be obtained.]]> 2018/12/13 9:30:20 LI Ne, ZHOU Wei-ya, ZHONG Mei, et al. 1048true ［Abstract］　Objective　To observe the effect of manual rehabilitation on somatosensory evoked potentials (SEP) in patients with cervical spondylotic radiculopathy (CSR).Methods　Forty CSR cases were divided into manual rehabilitation group and routine group equally. The SEP was examined before and 2 weeks after the rehabilitation treatment.Results　The total effective rate in manual rehabilitation group and routine group were 95% and 85% with the clinical healing rate being 60% and 40%，respectively. There was no significant difference in the therapeutic effect and the mean course of treatment between the two groups. The abnormal rate of SEP was 55% before the treatment. The main abnormality was the delay of the latent period of N9, N11andN13. The latent periods in both groups were shortened after treatment. Compared with the routine group, the latent periods of N11 in manual rehabilitation group were shortened significantly (P<0.05).Conclusion　Manual rehabilitation can improve the symptom in CSR patients.]]> 2018/12/13 9:30:20 JIANG Bin, QIN Guo-wei 1047true ［Abstract］　Objective　To evaluate the value of magnetic resonance angiography in carotid artery-extracranial segment stenosis of acute cerebral infarction. Methods　Magnetic resonance angiography(MRA) and digital subtraction angiography(DSA) were performed in 32 patients with clinical suspected carotid stenosis in acute cerebral infarction patients. Stenosis measurement according to NASCET criteria for DSA was used, and stenosis measurement for MRA according to NASCET and cross section criteria was used. Results　The stenosis measurement according to NASCET criteria for DSA was used, of the 128 carotid arteries assessed on MRA in 32 patients, the sensitivity, specificity, veracity of MRA according to NASCET was 63%, 100%, 78.1%, respectively, Kappa=0.593. The sensitivity, specificity, veracity of MRA according to cross section criteria was 63%, 100%, 79.7%, respectively, Kappa=0.622. Conclusion　Sensitivity, specificity and veracity of MRA are higher to estimate carotid artery-extracranial segment stenosis, and could be used as a screening test for the diagnosis and follow-up examination of the carotid artery stenosis. For mostly patients with carotid stenosis, which don′t need to DSA checking. But in some patients DSA should be performed.]]> 2018/12/13 9:30:20 CHEN Yuan, ZHONG Wei-zhang, MA Long-bai, et al. 1046true ［Abstract］　In order to see the privileges of the PBL teaching method in improving the educated peoples′ clinical analysis capability and skills, the researcher tried to use the PBL teaching method in the latter part of clinical surgery teaching. PBL teaching method pay more attention to cultivate the students′ ability to analyze and solve problems, so it succeed to make the education system shifting from exam-oriented mode to diathesis mode. Practice showed that this teaching method was coincide with the reformation in developing the students′ practical abilities and the spirit of innovation, and it is more suitable for the latter part of the work in clinical surgery teaching. The author proposed some specific solutions to solve the problems in the purposes/ designing questions/ panel discussing programs of the latter work in clinical surgery teaching. Meanwhile the author analyzed the necessity/advantages/deficiencies/problems in the latter part of clinical surgery teaching. Finally it discussed the developing prospects of the PBL teaching method.]]> 2018/12/13 9:30:20 TANG Qian-li, HE Jun, YANG Jian-rong, et al. 1045true ［Abstract］　Objective　To observe the effects of acute hypervolemic hemodilution （AHH）with different plasma substitutes on hemodynamics and renal funtion in neurosurgical patients.Methods　Sixty ASA Ⅰ～Ⅱ patients undergoing elective neurosurgery were divided into 3 groups(n=20 each):6% hydroxyethyl starch group (Voluven130/0.4) (group V), 5% Polygeline group(group P) and Lactated Ringer′s solution group(group R). After induction of anesthesia the patients were randomized to receive 20 ml·kg^-1 of either 6% Voluven, 5% Polygeline or Lactated Ringer′s solution within 30 min. Blood samples were taken before (T₁), immediately　at(T₂) and 30 min after the end fluid infusion (T₃) for determination of hematocrit(Hct)、hemoglobin（Hb）、urea nitrogen（BUN）、Creatinine（Cr）and β₂-mircroglobulin (β₂-MG). And during the hemodilution the heart rate （HR）, mean arterial blood presure （MAP） and central vein pressure （CVP） were continuousely monitored.Results　Lactated Ringer′s solution was significantly less efficient in expanding intravascular blood volume than the 2 plasma substitutes, but there no significant difference in blood volume expansion rate among group Vand P. In the AHH process three group of patient MAP and HR maintain stable relatively, CVP increased significantly after AHH compared with the baseline value before AHH in group V、P (P<0.01). There was no significant change in MAP and HR in all three groups. There was no significant change in BUN, Cr and β₂-MG.Conclusion　Voluven and polygeline are comparable in expanding blood volume and are more efficent than Lactated Ringer′s solution，hemodynamics is stable; Voluven and polygeline have no influence to the renal funtion and safety handling in neurosurgical patients.]]> 2018/12/13 9:30:20 LUO Xi-bao, LIU Zhi-gui, LIN Gao-xiang, et al. 1044true ［Abstract］　Objective　To find the effective technique of regulating abnormal activation of nerve and endocrine in the patient with chronic heart failure(CHF). Methods　Ninety-three patients with CHF were randomly prospectively divided into three groups and treated with Lotensin, Betaloc, Spironolactone(group A), and Qiliqiangxin capsule(group B) and basic treatment group respectively and observed which was better. Results　Group A was went down the level of renin, angiotensin Ⅱ, aldosterone on patients with CHF. Group B is not more went down the levels, but it can improve heart function. Conclusion　Lotensin, Betaloc, Spironolactone are effective on regulating abnormal activation of nerve and endocrine in the patient with CHF. Qiliqiangxin capsule is not better than Lotensin, Betaloc, Spironolactone on regulate abnormal activation of nerve and endocrine in the patient with CHF, but it can improve the quality of life and heart function.]]> 2018/12/13 9:30:20 KUANG Jin-bo, HUANG Run-jian, PAN Ai-qing, et al. 1043true ［Abstract］　Objective　To investigate the analgesic effect of automatic control intravenous analgesia with a small-dose remifentanil in parturient women and its effect on delivery outcome.Methods　Twenty-seven parturient women were administered automatic control intravenous analgesia with a small-dose remifentanil （observation group）and thirty parturient women did not receive this analgesia(control group).Their delivery outcome were compared.Results　The analgesia effect of the observation group was better than that of the control group (P<0.01).There were no difference in using of oxgtocic hormone, cesaren section rate, postpartum hemorrhage and Apgar score of neonates between the two groups(P>0.05).Conclusion　The automatic control intravenous analgesia has advantages of fast effect, good analgosic effect, less physiologic effect on parturient women, no harmuful effect on fetal, and being accepted by parturient women, and is worth popularizing.]]> 2018/12/13 9:30:20 LIU Zi, ZHOU Rui-ren 1042true ［Abstract］　Objective　To discuss the effect of mild hypothermia on expression of NMDAR1 in rats′ different cerebral tissue after cardiopulmonary resuscitation. Methods　Model of CPR rats were found, and expression effect of mild hypothermia of NMDAR1 after CPR rats′ different cerebral tissue were observed by immunohistochenistry. Results　After CPR NMDAR1 expression in rats′cerebral different tissue in mild hypothermia group was lower significantly than that in control group and had marked difference (P<0.01). Conclusion　Mild hypothermia can decrease the expression of NMDAR1, decrease postive ion concentrition and thus decrease the cerebral edema, so mild hypothermia can treat cerebral edema after CPR.]]> 2018/11/7 8:23:04 SUN Zhi-yang, LI Guang, SUN Qing-wen, et al. 1041true ［Abstract］　Objective　To observe the effects of 2, 3, 5, 4′-tetrahydroxy-stilbene-2-O-β-D-glycoside (TSG) on learning and memory abilities and free radical scavenging in senescence accelerated-prone mouse/8 (SAMP8).Methods　Fifty 6-month-old SAMP8 mice were randomized to 5 groups: SAMP8 group, huperzine A control group, low-, mid- and high-dose groups of TSG, with 10 mice in each group. Another 10 6-month-old SAMR1 mice were assigned to normal control group. After medication for 50 days, Morris water maze test was used to assess the change of learning and memory abilities and spectrophotometry was used to detect SOD and GSH-Px activity and MDA contents in each mouse brain.Results　Compared with normal control group, SAMP8 mice showed significant impairments in learning and memory in hidden platform test, and the escape latency was significantly longer than that of TSG-treated group（P<0.05）. TSG-treated group could significantly increase the time and times of swim in original platform quadrant in spatial probe test（P<0.05）. SOD and GSH-Px activity decreased significantly and MDA contents increased significantly in SAMP8 brain. Compared with the SAMP8 group, TSG-treated groups could enhance SOD and GSH-Px activity and decrease MDA contents significantly in SAMP8 brain (P<0.05).Conclusion　TSG could improve the learning and memory abilities and scavenge free radicals effectively in SAMP8 mice.]]> 2018/11/7 8:23:04 HUANG Ri-cai, HUANG Zhong-shi, LI Yun, et al. 1040true ［Abstract］　Objective　To study the effects of jianling decoction on apoAI, apoB and Lp(a) in hyperlipidemic rats.Methods　Sixty SD rats were randomly divided into five groups: control group, model control group, jianling decoction group, jianzhiling capsule group and Simvastatin group. Each group had 12 rats and were treated differently. The concent of blood apoAI, apoB and Lp(a) were determined.Results　Jianling decoction obviously reduced the contents of blood apoB and Lp(a), it raised the contents of blood apoAI.Conclusion　Jianling decoction can affect the metabolization of serum lipid through raising apoAI and reduing apoB.It could reduce Lp(a) so that it can prevent atheroselerosis.]]> 2018/11/7 8:23:04 FENG Dao-rong, YANG Jia-zhen, SUN Yi, et al. 1039true ［Abstract］　Objective　To research on the change of brain natriuretic peptide and respiratory index in perioperative period of open heart surgery and its clinical significance.Methods　In 45 patients underwent the first time cardiac valve replacement, at 30 min before operation, CPB 30 min, downtime, the downtime 1 h, 1 d and 7 d after operation，the synchronous monitoring of the brain natriuretic peptide, cardiac troponin T, respiratory index were performed, and the relevance of the research indicators were analysed.Results　The level of brain natriuretic peptide after cardiopulmonary bypass surgery were significantly increased to a peak value at 1 d after operation, at 7 d after operation brain natriuretic peptide levels decreased, but still significantly higher than preoperative levels (P<0.01); respiratory index after cardiopulmonary bypass surgery were significantly increased and reached the peak value at 10 mins of cardiopulmonary bypass (P <0.05 or P<0.01), and dropped to preoperative levels at 7 d after operation (P>0.05). There was a positive correlation between brain natriuretic peptide and cardiac troponin T (r= 0.48, P<0.05); respiratory index and brain natriuretic peptide, cardiac troponin T did not show the relevance (r=0.23, r=0.17, P> 0.05).Conclusion　Synchronous monitoring of brain natriuretic peptide and respiratory index can to provide a basis for the protection of perioperative cardiopulmonary function.]]> 2018/11/7 8:23:04 LIN Shan-wen, SU Yue-nan, ZHANG Sheng-sun, et al. 1038true ［Abstract］　Objective　To study the infection status of HIV on pregnant women, and to explore an effect approaches to reduce maternal to child transmission with HIV.Methods　Pregnant women were provided free counseling service and HIV testing, and adopting comprehensive measures of the interruption of maternal to child transmission of HIV to pregnant women who with HIV positive and willingness of child-bearing.Results　Fourteen thousand，eight hundred and thirty-three pregnat women was provided free counseling service with a counseling rate of 84.80%, 24 out of 12 407 detected women were found HIV positive with a positive rate of 0.19%, and after opting comprehensive measures of the interruption of matemal to child transmission of HIV to pregnant women, 15 out of 16 infants who were born by 16 out of 24 HIV positive women showed HIV negative during a period of HIV-DNA.Conclusion　HIV can be transmitted to fetus or infants during pregnancy, delivery and breast feeding, opting comprehensive measures of the interruption of matemal to child transmission of HIV to pregnant women can prevent HIV infection of newborn.]]> 2018/11/7 8:23:04 YANG Zhi-juan, HUANG Shu-liang 1037true ［Abstract］　Objective　To study the bacteriostasis and its practical safety of mango seed extractive in vivo and vitro.Methods　The bacteriostasis in vitro of mango seed extractive, double-diluted and agar-diffused, on common intestinal infection pathogenic bacteria（standard and tolerated）: Bacillus shigae, Shigella flexneri, Escherichia coli were observed; the bacteriostasis in vivo of mango seed extractive were observed on duplicated infected model mouse; the acute toxicity test was conducted in fasting mouse gavaged with mango seed extractive.Results　Mango seed extractive possesses bacteriostasis in vitro of different degrees on standard and tolerated Bacillus shigae, Shigella flexneri and Escherichia coli, respectively with minimal inhibitory concentrations 7.8 mg/ml, 7.8 mg/ml, 7.8 mg/ml, 15.6 mg/ml, 15.6 mg/ml, 15.6 mg/ml and with bacteriostasis collar diameters 20 mm, 18 mm, 21 mm, 19 mm, 17 mm, 18 mm. Large, medium and low dosages had evident bacteriostasis on the mice infected with Shigella flexneri and Bacillus shigae. Maximal tolerated dose of intragastric administration for the mouse was 40 g/kg, (equivalently 50 times clinic dosage). Conclusion　Mango seed extractive possesses evident bacteriostasis and favorable safety in vivo and vitro on 3 types of common intestinal infection bacteria.]]> 2018/12/3 8:39:12 MO Wu-gui, LIU Hua-gang, ZHENG Chen-guang, et al. 1036true ［Abstract］　Objective　To investigate the variation of myopic point spread functions after conventional LASIK.Methods　Thirty five myopic out-patients (male 18, female 17) underwent conventional LASIK between March and July 2008 in Guangxi visual science and optometry center, aged from sixteen to thirty six years, and refractive power ranged from -0.50 to -10.50 diopters(spherical equivalent) were enrolled retrospectively. WaveScan^® system(Visx corp. made in USA) was used to capture each myopic wavefront aberration images at pre-LASIK, post-1 day, 1 week, 1 month, 3 months and 6 months after conventional LASIK, respectively. Efficient blur was used as a parameter of myopic optical quality. One-way ANOVA and LSD were analyzed to compare point spread function values among the six time points by SPSS 13.0 version software.Results　All 35 myopic wavefront aberrations examined by WaveScan system showed that myopic PSF values after LASIK were different from each other, and statistic significant differences were found between pre- and post-LASIK(all P<0.05), but there were no significant differences in PSF among each time points after LASIK(P>0.05).Conclusion　LASIK could drastically improve myopic visual quality. And myopic PSF values keep relatively stable at early period after conventional LASIK.]]> 2018/12/3 0:00:00 HUANG Jian-zhong, ZHAO Wu-xiao, LIU Wei-min, et al. 1035true ［Abstract］　Objective　To explore the mode of oral care for 0-3 years′ old kids.Methods　Sixty cases were randomly chosen as experiment group which health education and behavior were carried out among pregnant mothers and their husbands. After the children had born, they must be had a check of oral health periodically, and their parents should get some advices about their childrens′ oral health individually. Some preventive measures should be adopted if necessary.Another sixty kids were chosen randomly as the control group from primary classes of one kindergarten without any intervention. Two groups were checked of oral health at 3 years′ old, and all mothers were investigated with questionnaire. The resulting data were entered by EpiData3.0 and processed by the statistic software SPSS12.0.Results　There was a significant difference on the oral health care knowledge level among the mothers between the experiment group and the control group (P<0.05). Dental caries rate was 10%in experiment group while 25% in control group and the difference between the two groups was significant too (P<0.05).Conclusion　The mode of health education and a series of preventive measures on oral health is effective and has a value of clinical spreed application.]]> 2018/12/3 8:39:12 SHI Chun-mei, ZHOU Yan, WEI Hui-ping, et al. 1034true ［Abstract］　Objective　To explore the clinical manifestations and chest X-ray findings of acute respiratory distress syndrome (ARDS) in newborns.Methods　The data of 14 newborns with ARDS were retrospectively analysed, the data included chest X-ray findings, change in part of the review, clinical symptoms, and blood gas performance.Results　X-ray findings included lung permeability decreased brightness, with ground-glass-shaped in 10 cases; lungs diffuse pacth exudative lesions in 4 cases of; large sheet exudative lesions in 1 cases, bronchial inflatable sign in 5 cases; “white lung” in 3 cases; of 3 cases which were reviewed, improvement of the performance in 2 cases, increase in the 1 cases; and X-ray findings was basically identical with clinical and blood gas findings.Conclusion　The X-ray manifestations of the newborns of ARDS has its own characteristics, is an important diagnostic indicators; X-ray findings, clinical symptoms, and blood gas reflects their performance of a certain stage.therefore, the pre-and post control and their combination was important to the diagnosis of ARDS.]]> 2018/12/3 8:39:12 YOU Jian-xiong, WEI Xiong, CHEN Wen-rui, et al. 1033true ［Abstract］　Objective　To evaluate the clinical efficacy of temporary conjunctival flap treating fungal corneal ulcer.Methods　Fifty-one cases(51 eyes) with fungal corneal ulcer were treated with temporary conjunctival flap implantation, the follow up time was 6 months after operation. Results　Of 51 cases in this group, 49 cases were cured and treatment failure occurred in 2 cases, the cure rate was 96.08%.The average healing time was （14.10 ± 4.81）days.Corneal neovascularization occurred in 2 cases (4.08%). The postoperative uncorrected visual acuity was ≥0.3 in 19 cases (37.25%), 0.02～ 0.1 in 21 cases, the permanent loss of vision in 2 cases of treatment failure, the visual acuity impoved to various extent in the rest. Conclusion　Temporary conjunctival flap could promote fungal corneal ulcer healing, and corneal neovascularization was less，but in serious cases，the efficacy is not ideal.]]> 2018/12/3 8:39:12 YU Ye-ming, LI Yan-chun, MO Yi-juan, et al. 1032true Methods　A total number of 2 305 cases received serum test for TORCH-IgM antibody with ELISA techniques, 170 cases were randomly selected from the positive results which were divided into observe group (85 cases)and control group(85 cases), Bu Qi Jie Du Soup treatment was uesd in observe group, another group adopted common Western medicine，the difference between these two groups were analysed.Results　The positive rate of TORCH infection was 7.38% in Liuzhou, the positive rates of IgM for CMV、TOX、RV、HSV-Ⅰ, and HSV-Ⅱwere 3.90%, 1.82%, 1.04%, 0.35% and 0.26% respectively, there was statistical significance in the differences of infection rate in different pathogens（P<0.05）, CMV was the first in TORCH prevalence;There was no statistical significance in the differences of curative effect between observe group and control group（P>0.05）, Bu Qi Jie Du Soup treatment for TORCH infection had the same curative effect with common Western medicine treatment.Conclusion　The study of the prevalence of TORCH infection among childbearing aged women in Liuzhou is helpful to guide the aristogenesis works in liuzhou; Bu Qi Jie Du Soup treatment is against several kinds of virus infection, and the time of treatment shortened，it is effective in preventing or reducing bad pregnancy, it is worth to extend and apply in clinical work.]]> 2018/12/3 8:39:12 OU Qi-wen, WANG Qiong, MO Ke-liang, et al. 1031true ［Abstract］　Objective　To investigate the role of Thr284 in the catalysis of Cathepsin E.Methods　Replace Thr284 with a serine to produce a T284S mutant by site-directed mutagenesis.Results　Both wild type and mutant are expressed and present in the HEK 293T cell culture media and cell lysate. Mutant is still able to auto activate, although its activity is only 40% of that of wild type. The Km value of mutant is similar to that of wild type, but its Kcat value is smaller than that of wild type. Both mutant and wild type are inhibited by pepstatin and Ascaris pepsin inhibitor with similar Kivalues. The CD spectra of mutant and wild type is similar.Conclusion　(1)Thr284 is very important to the catalytic activity. The mutant causes change of hydrogen bonds distribution and thus lowers the hydrolysis efficiency. (2)Other protein structural features of BACE-1 than its carboxyl motif may contribute to its insensitivity to pepstatin.]]> 2018/12/3 8:39:43 LIU Jian 1030true ［Abstract］　Objective　To study the impact of the treating dose of target volume in different radiotherapy plans on radiation situation of the brain of patients who suffer from nasopharyngeal carcinoma (NPC) with magnetic resonance imaging (MRI) and computed tomography（CT） image difference in cranial base.Methods　According to the MRI and CT image difference in cranial base, we applied ARTP-TOP3D-TPS system to delineate target volume and develop the three-dimensional conformal radiotherapy (3-DCRT) plans. Tewenty-six NPC patients were treated by the 3-DCRT plans. The mean dose and D5 mean dose of the target volume in the two group(CT based plan and MRI based plan) were compared.Results　Compared with CT based plan（34.66±4.99）Gy, （52.11±7.15）Gy, （5.66±3.95）Gy, （6.36±4.11）Gy, （29.94±5.69）Gy, （30.23±6.54）Gy, the mean dose of brainstem, pituitary, temporal lobe and both eyes in MRI based plan（47.01±4.61）Gy, （59.59±8.80）Gy, （5.92±3.72）Gy, （6.55±3.80）Gy, （38.27±6.15）Gy, （38.78±7.52）Gy were larger (P<0.05). There was no statistic difference in the mean dose of spinal cord between two plans［MRI （29.51±5.80）Gy, CT（30.30±4.04）Gy］. Compared with CT based plan（56.49±2.52）Gy, （41.51±4.65）Gy, （54.04±7.31）Gy, （20.17±10.47）Gy, （19.37±11.44）Gy, （60.69±3.62）Gy, （60.04±6.84）Gy, the D5 mean dose of brainstem, spinal cord, pituitary, temporal lobe and both eyes in MRI based plan（66.15±3.43）Gy, （44.26±4.35）Gy, （61.59±8.89）Gy, （20.65±10.66）Gy, （20.09±11.65）Gy, （67.60±2.39）Gy, （67.31±6.88）Gy were larger (P<0.05).Conclusion　The dose of target volume in 3D-CRT plan based MRI is superior to CT. The dose of important organ of the brain in 3D-CRT plan based MRI is higher.]]> 2018/12/3 8:39:43 QIN Ling, LI Ling, WANG An-yu 1029true ［Abstract］　Objective　To investigate the anxious and depressive state of patients with brain tumors during the process of radiotherapy and influential factors.Methods　Patients were investigated, evaluated and analyzed with self-rating depression scale, self-rating anxiety scale and relative factors questionnaire.Results　The affective disorder both anxiety and depression degree of patients with brain tumors during the process of radiotherapy was significantly higher than that of health adult.Conclusion　The affective disorder including anxiety and depression exist in patients with brain tumors during their process of radiotherapy and result from multiple factors.]]> 2018/12/3 8:39:43 ZHU Shou-hong, HUANG Xin, ZHANG Jian-wen, et al. 1028true ［Abstract］　Objective　To study the possible relationship between the point mutation in mitochondrial DNA(mtDNA) and the progression spinocerebellar ataxia(SCA).Methods　Polymerase chain reaction(PCR) was used to amplify the mtDNA segments of these patients and their relatives individuals, 40 volunteers. The mtDNA segment lied in the above mtDNA ND5 gene. For PCR products of rhe mtDNA segment, single strand conformation polymorphism(SSCP) was executed to detect mutations and the abnormal segments were sequenced.Results　We had found a new mtDNA mutation in segments of mtDNA point 13731（T>C）, was identified in 1 patient and 1 presymptomatic relatives.Conclusion　A new point mutation of detected mitochondrial DNA may be lated to SCA.]]> 2018/12/3 8:39:43 WANG Dong-hui, WANG Jin 1027true ［Abstract］　Objective　To study the effect of atorvastatin on urine protein(UAER), endothelin(ET), nitrogen monoxidum(NO)，C-Reactive protein(CRP) in older patients with early diabetic nephropathy(DN) without hyperlipemia.Methods　Fifty older DN without hyperlipemia were randomly divided into two groups, the conventional treatment group (n=25), conventional treatment plus atorvastatin group (unitive group, n=25), and 25 normal controls were chosen as normal control. The plasma serum lipids (CH, TG, LDL, HDL), ET, NO, CRP and urinary albumin excretion rate(UAER) were examined before and after the treatment.Results　The levels of ET, CRP in patients with DN was higher than those in normal control group, and the level of NO was lower. After 12 weeks of treatment，the levels of serum lipids, ET, NO and UAER had no changes in the conventional treatment group, but they were improved in unitive group.Conclusion　Atorvastatin can decrease the levels of UAER in older patients with early diabetic nephropathy, which may be due to its correction of abnomal renal hemodynamics through decreasing ET and increasing NO, improving serum lipids, lessening inflammatory reaction.]]> 2018/12/3 8:39:43 Ll Jian -ying, GUO Jin, WANG Zhi-ying, et al. 1026true ［Abstract］　Objective　To investigate the influence of pulp status on the accuracy of determining root canal length by TRR1 electronic apex locator in clinic.Methods　Two hundred and fifty-seven root canals of 87 teeth were divided into two groups, teeth in group A were diagnosed as pulpitis, and teeth in group B were apical periodontitis. All these root canals′ working length were measured by TRR1 electronic apex locator, then measured the distance between the K-file tip and the radiographs(L), when 0 mm≤L≤2 mm, the data were considered accuracy. Results　The accuracy rate of group A was lower than that of group B, but there was no significant difference in these data between two groups. Conclusion　The accuracy of TRR1 electronic apex locator wasn′t influenced by the pulp status in this study.]]> 2018/12/3 8:39:43 LI Yan, TANG Ji-wei, LU Zhao-wei 1025true ［Abstract］　Objective　To observe the clinical curative effect and the change of hemorheology of xuemaitong capsule in the treatment of the chronic obstructive pulmonary disease (COPD) patients with hyperviscosity at stable stage. Methods　Sixty confirmed COPD patients with hyperviscosity were randomly into two groups. Thirty patients of the treated group were treated with the xuemaitong capsules. Another 30 patients of the control group were treated with aspirin enteric-coated tablets and theophylline sustained-release tablets. Pulmonary function and the changes of hemorheology were observed before and after treating for one month.Results　The compared with the control group, the treated group was superior in the clinical effect, the improvement of pulmonary function and hemorheology (P<0.05). Conclusion　Xuemaitong capsule can effectively improve the clinical curative effect and status of hemorheology in COPD patients with hyperviscosity, and delay the deterioration process of pulmonary function of the patients.]]> 2018/12/3 8:39:43 WU Zi-feng, JIN Ying 1024true ［Abstract］　Objective　To observe the effects of hemoperfusion on refractory hypertension and the material caused hypertension in hemodialysis patients. Methods　Sixty hemodialysis patients with refractory hypertension were divided into two groups randomly. On the base of hemodialysis, hemoperfusion group was given hemoperfusion; control group was given hemodiafiltration. The blood pressure 、PRA、ET、ATⅡ、PTH、Ald were observed.Results　Two groups had high level of PRA、ET 、ATⅡ、PTH、Ald. 16 weeks later, Bcr、P、β₂-MG、Ald were significant decreased in both groups(P<0.05); ATⅡ、ET were significantly decreased in hemoperfusion group(P<0.05); the level of PTH in hemoperfusion group was significantly lower in hemoperfusion group(P<0.01), and in control group(P<0.05). The blood pressure and medicines were significantly decreased in hemoperfusion group.Conclusion　Hemoperfusion treatment had good effects on cleaning material caused hypertension and decreasing hypertension.]]> 2018/12/3 8:39:43 ZHANG Wen-xin, PENG Xiao-mei, GONG Zhi-feng, et al. 1023true ［Abstract］　Objective　To study the change of serum levels of carbohydrate antigen 125（CA125）in patients with chronic heart failure(CHF)and its clinical significance.Methods　Thirty-two patients with CHF were divided into two groups based on standard New York Heart Association classification. NYHA I and II was known as the A group and 17 patients was in the A group.NYHA Ⅲand Ⅳwas known as the B group and 15 patients was in the B group.Twenty-five healthy persons were recruited as control group, The serum CA125 levels were determined by electrochemical luminescence immunoassay.Results　The level of CA125 in the patients with CHF was significantly higher than that in the control group.The level of CA125 in B group was higher than that in A group, but no significant difference（P>0.05）. Conclusion　There was relevance between CA125 level and NYHA. The level of CA125 maybe a good index for CHF diagnosis and assessment.]]> 2018/12/3 0:00:00 WANG Ruo-qi, LI Tian-zi, LUO Zhang-wei, et al. 1022true ［Abstract］　Objective　To investigate the expression of matrix metalloproteinase-9 (MMP-9) in human tongue squamous cell carcinoma（Tca8113）in vitro and in vivo, after the expression of vascular endothelial growth factor（VEGF）suppressed by vector-based small interfering RNA(siRNA).Methods　The eukaryotic expression vector taken as an experimental control and two siRNA targeting VEGF constructed in the vector (VEGF-siRNA1, VEGF-siRNA2) were transfected into human tongue squamous cell carcinoma cell lines(Tca8113). The non-transfected Tca8113 cells were used as negative control. The transfected cells and non-transfected cells were injected subcutaneously on the back of the twenty nude-mice which were divided into four groups randomly (n=5/group), respectively. The expression of VEGF and MMP-9 in the cultured cells and the xenograft tumors were detected by immunocytochemistry and immunohistochemistry.Results　Compared with the experimental and negative controls, the VEGF expressions were decreased (P<0.05) in two experimental groups of the cultured cells and xenograft tumors. There was no obvious MMP-9 staining in four groups of cultured cells. There were no significant differences of the MMP-9 expression among four groups of xenograft tumor (P>0.05).Conclusion　VEGF-siRNA can down-regulate the expression of VEGF in the human tongue squamous cell carcinoma in vitro and in vivo. However, it may not influence on the expression of MMP-9 efficiently.]]> 2018/12/6 8:49:55 HAO Jie, YU Da-hai, LI Jing, et al. 1021true ［Abstract］　Objective　To explore the origin of vascular endothelial cells in human tongue carcinoma xenograft model by nude mice.Methods　The model of human tongue carcinoma xenograft was established by transplanting Tca8113-Ml subcutaneously to nude mice, to identify the germinal origin of neovascularization and assay the microangiogenesis, the neoplasms(>1cm in diameter) induced by Tca8113-Ml were removed and dealt with histopathologically through immunohistochemical staining of VEGF and CD34(including rat -anti-human and rat-anti-mouse CD34).Results　HE staining showed squamous cell carcinoma; positive expression was observed in rat-anti-mouse CD34 staining and MVD averaged 10.72±2.12, meanwhile no visible alteration of rat-anti-human CD34 staining;moreover, VEGF staining presented a 100% positive ration in six transplanted tumor and the numbers of positive cells averaged (67±5.6)% in every slice.Conclusion　The germinal origin of vascular endothelial cells in Tca8113-Ml xenograft model derived from host nude mice, and may be induced by VEGF which is secreted by tumor cells.]]> 2018/12/6 8:49:55 ZHU Ming-yi, KUANG Xiao-cong, HUANG Ying-hua, et al. 1020true ［Abstract］　Objective　To discuss the biological properties of human bone marrow derived mesenchymal stem cell (hBMSC) after long term in vitro culture and it′s malignant transformation.Methods　hBMSCs were isolated and enriched by the gradient centrifugation protocol and cultured in vitro for long term. During in vitro culture, the cell line′s morphology were observed; It′s differentiation were evaluated; The expression of cancer relative markers: P53、Ki67 were detectived, and the tumor-initiate ability were assessed.Results　When culture to the 36th passage, hMSC were gradually aging, cannot differentiate to ossification, cancer relative markers were still negative, there was no tumor initiation.Conclusion　hBMSCs can be cultured in vitro for limited time, there was no significantly malignant transformation during the limited term.]]> 2018/12/6 8:49:55 HUANG Ying-hua, WEI Ying, CAI Jie, et al. 1019true ［Abstract］　Objective　To investigate the clinical effect of everStick high-strength glass fibers posts core crown on crown fracture restoration of immature permanent tooth. Methods　Forty-eight patients with 62 crown fracture were selected.Vitapex was used in apexification, and the residual crowns and roots were restored with the everStick high-strength glass fibers post core crown.The patients were reexamined at 3th, 6th, 9th, 12th month after operation.Results　Of 62 teeth, 58 were successful at 12th month after operation, the successful rate was 93.5%.Conclusion　The apexification and the everStick high-strength glass fibers post core crown could meet the clinical need of fracture restoration of immature permanent tooth.]]> 2018/12/6 8:49:55 LIU Guang-xue, PENG Li-hui, PAN Xiao-bo, et al. 1018true ［Abstract］　Objective　To analyze differential expression protein in the patients with multiple myeloma by proteomic technology.Methods　Serum protein from 30 patients with multiple myeloma at preliminary diagnosis(MM group) and 33 healthy people control group were analyzed by CM10 proteinchip array and surface-enhanced laser desorption-ionization time-of-flight mass spectrometry(SELDI-TOF-MS).Ciphergen Biomarker wizard software was used to identify proteomic features. Results　The analysis of the protein map showed, there was siginificant difference between two groups(P<0.05) in 30 protein peaks. Compared with the control group, 10 protein peaks were up-regulated expression and 20 prtotein peaks were down-regulated expression in MM group.Five prtotein peaks (3472.79、7778.39、4093.01、4971.15、5343.98)were of more significance. Conclusion　The comparison between the patients with multiple myeloma and healthy people in the serum protein is helpful to screen serum specific markers of multiple myeloma.]]> 2018/12/6 8:49:55 TANG Juan, PENG Kuan, LI Shan 1017true ［Abstract］　Objective　To study the effects of (CSII) on the first-phase insulin secretion function of β cell in newly diagnosed type 2 diabetes patients.Methods　Fourty-two newly diagnosed type 2 diabetes patients were randomly divided into therapy group and control group. The twenty-one patients in therapy group were treated with CSII, while 21 patients in control group were treated with insulin injection alone. Area under the curve of insulin (AUC) during IVGTT were compared between therapy group and control group. Results　The AUC in control or therapy groups increased after week 2 of the treatment, and the AUC in therapy group were significiantly better than those in control group(P<0.01). Conclusion　CSII in the treatment of newly diagnosed diabetes is effective for raising the first phase insulin secretion funnction of β-cell.]]> 2018/12/6 8:49:55 CHEN Gen-ben, CHEN Ru-zi, CHEN Jian-zhen, et al. 1016true ［Abstract］　Objective　To evaluate the application of transvaginal color Doppler ultrasonography (TVS-CD) in differential diagnosis of benign and malignant ovarian tumor.Methods　A total of 92 cases with solid and cystic-solid mixed ovarian masses were performed transvaginal color Doppler ultrasonographic examination and the characteristic of gray-scale sonogram and haemodynamics of the tumor arteries were analyzed. The sonographic diagnosis was compared with the histopathology after operation. Results　The sensitivity and specificity of malignant ovarian tumors with diagnostic criteria of resistance index (RI)<0.5 for 71 cases which demonstrated tumor arteries were 96.6% and 93.3%, respectively. And the diagnostic sensitivity and specificity of malignant ovarian tumors with diagnostic criteria of RI<0.5 for all of 92 cases were 90.0% and 90.3%, respectively. Conclusion　The parameter of RI may improve the diagnosis of malignant ovarian tumor. And the TVS-CD is a useful technique in differential diagnosis of benign and malignant ovarian tumor.]]> 2018/12/6 8:49:55 ZHENG Hong-yu, SHEN Gui-xin, KANG Li-ke, et al. 1015true ［Abstract］　Objective　To analyze the data obtained in hearing screening in newborn intensive care unit（NICU）, in order to acquire the positive rate of distortion product otoacoustic emission（DPOAE）in the various diseased neonates in NICU. Methods　The first screening was performed in third day after birth by the equipment of otoacoustic emission of GSI-70, the failed one should be performed the second screening after 42 days and the first audiometry after the third month was performed.The normal neonates was sened as the compare group.Results　The positive rate of newborns with disease in NICU were obviously higher than that in compare group(P<0.01). Among the positive rates there was a highest rate in premature infant(P<0.05）.The positive rates were as follows:the premature infants, 55.5%; the lower weight, 52.2%;the pneumonia asphyxia, 42.3%;the great neonates, 41.4%;the rest of high risk，35%, and the infants Hyperbilirubinemia, 33.2%.Conclusion　The positive rate of hearing screening of neonates with OAE is clearly higher than the normal newborn, and it has difference in the types of diseases but not sex.It is valuable to have a tracing care for babies who with disease high positive rate.]]> 2018/12/6 8:49:55 ZHAO Heng-jing, LI Hong-hui, SU Qong-li 1014true ［Abstract］　Objective　To explore the value of the evaluation of cervical ripeness and the prediction of premature delivery by B ultrasound measurements of cervical length and width of cervical internal opening. Methods　The B ultrasonographic examination of 187 hospitalized primipara with singleton fetus were performed to measure the length of uterine cervix and width of cervical internal opening after filling of bladder. Results　The length of uterine cervix were <30 mm, width of cervical internal were ≥5 mm, the risk of premature delivery increased.Conclusion　The ultrasonogyaphic exam is an objective, effective, and repeatable method in predicting premature delivery.]]> 2018/12/6 8:49:55 SU Dong-lu, JIANG Jun -song, WEI Jing-qiu, et al. 1013true ［Abstract］　Objective　To evaluate the clinical effectiveness of small dosage cyclophosphamide (CTX) and plasm exchange (PE) in treating serious systematic lupus erythematosus (SLE).Methods　Sixty patients with serious SLE in our hospital were divided into treating group and contrast group. Small dosage of CTX + PE treatment was applied to patients in the treating group, while large dosage of CTX impulse treatment was applied to patients in the contrast group. The clinical symptom (erythema in the face, pain in the joints, febricity) and index in activities (anti-ds-DNA antibody, ANA, Scr, 24-hour urinary protein, erythrocyte sedimentation rate, and complement C₃) changes after treatment were observed. Results　Compared with the contrast group, the releasing rate of clinical symptom in the treating group was higher and faster, the index of activities was of significant difference (P<0.05). Conclusion　Plasm exchange could rapidly control the activity of serious SLE, combined with application of small dosage cyclophosphamide would improve the short term and long term clinical effectiveness, and decrease side effects.]]> 2018/12/6 8:49:55 HUANG Wei-hua, NING Jun, HUANG Yi-jiang, et al. 1012true ［Abstract］　Objective　To evaluate the analgesia effects of using non-nutritive sucking（NNS） method among neonatal infants，and investigate nursing intervention method.Methods　One hundred neonatal infants in neonatal intensive unit (NICU), whose gestational age greater than 33W, were randomly divided into the control group and the NNS group, and all received pain stimulation of heel pinprick for blood.Besides, non-nutritive sucking was induced in the neonate of NNS group. The pain degree of the neonates were measured by duration of crying, neonatal infant pain scale (NIPS) at the points of 0.5, 1 and 5 minutes after stimulation, and the fluctuation of heart rate and oxygen saturation.Results　There was significant difference between the 2 groups on the pain degree: NNS group had shorter duration of crying（P<0.01）, lower NIPS points（P<0.01） and more small changes of heart rate and oxygen saturation（P<0.05） compared with control group. Conclusion　Non-nutritive sucking nursing intervention can effectively reduce the neonatal pain response to heel pinprick operations.]]> 2018/12/6 8:49:55 TANG Xiao-yan, ZHEN Hong, WEI Yu-hua 1011true ［Abstract］　Hepatocellular carcinoma (HCC) is one of the most frequent cancers worldwide. Effective therapy to this cancer is currently lacking, creating an urgent need for new therapeutic strategies for HCC. Gene therapy approach that relies on the transduction of cells with genetic materials, such as oncolytic viral vectors，anti-oncogenes, suicide genes, genes coding for antiangiogenic factors, small interfering RNA(siRNA), or genes coding for immunomodulatory molecules may provide a promising strategy. The aforementioned strategies have been largely evaluated in the animal models with HCC or liver metastasis. Due to the diversity of vectors and therapeutic genes, being used alone or in combination, gene therapy approach may generate beneficial effects to control the growth of tumors within the liver.]]> 2018/12/6 8:50:23 ZHOU Zhong-ping, HE Shao-jian 1010true ［Abstract］　Ventilator-associated pneumonia (VAP) keeps high frequency and results in increase in mortality. Management of ventilator circuit changes has become the strategy to prevent VAP. The relation between interval of changing ventilator circuits and VAP is not very clear. Changing ventilator circuits every 8 hours, 24 hours and 48 hours were recommended in the 60′s, 80′s and early 90′s in the last century, respectively. Later studies found no increase in VAP with more extended circuits change interval. This made the interval of changing ventilator circuits come to 7 days, or even more since the mid-90′s of the 20^th century. The correlations of circuits change interval and bacterial colonization of circuits, bacterial colonization and VAP，need more researches.]]> 2018/12/6 8:50:23 QIN Zhi-qiang, HUANG Gui-xiang 1009true ［Abstract］　Recurrent spontaneous abortion is one of the frequent complications in early pregnancy. Its incidence rate is roughly 0.4%～1%. The reasons of more than 80% of the cases are not clear. Few patients are suffered as a result of chromosomal anomaly, malformation in the reproductive organs, infection and so on. The role of infection in recurrent spontaneous abortion is gradually emphasized. This paper will give a brief introduction of the relationship between the etiology of recurrent spontaneous abortion and vaginal infection so as to provide basis for the prevention and therapy of recurrent spontaneous abortion.]]> 2018/12/6 8:50:23 LAO Yi-ping, PENG Ling-xiang 1008true ［Abstract］　Great achievments have been made in excimer laser corneal refractive surgery after aproximately twenty years′ revolution. In this article, we mainly summarized some new surgical methods, new technologies and their characteristics in the corneal refractive surgery area.]]> 2018/12/6 8:50:23 HUANG Jian-zhong, ZHAO Wu-xiao, YANG Jia 1007true ［Abstract］　Recent years, research of plasma exchange, immunosorbent techniques, leukapheresis therapy in　the treatment of rheumatoid arthritis is proved effective. This article summarizes the application of immunologic-purging therapy of rheumatoid arthritis.]]> 2018/12/6 8:50:23 ZHU xia，LIN Jing- ying 1006true ［Abstract］　At present, it is considered that the bacterial flora in laser in situ keratomileusis(LASIK) mainly root in conjunctival sac. The major pathogens which are including of bacteria, virus and fungus can result in infectious keratitis after LASIK. During all of course of operating, providing against infection can effectively control emergence of infectious keratitis. Now，the most effective treatment on infectious keratitis is appling relevant antibiotics according to their sensitivities. If under control of antibiotics, it is necessary to use subflap irrigating and corneal surgical treatment(including resection of corneal flap, transposition of lamellar corneal).]]> 2018/12/6 8:50:23 LAN Fang-Fang, LIU Xian-zhou, LIU Wei-min 1005true ［Abstract］　Objective　To explore the relationships between type 1 diabetic encephalopathy and neurogenesis in the hippocampal dentate gyrus, and the effects of insulin replacement therapy on the disease.Methods　 Streptozotocin-induced type 1 diabetic encephalopathy and insulin replacement therapy models of Wistar rats were respectively developed. Morris water maze, bromodeoxyuridine(BrdU), BrdU+NF(neurofilament), and BrdU+GFAP (glial fibrillary acidic protein) immunohistochemistry were used to detect the spatial learning and memory, the survival, migration and differentiation of neural stem cells in the hippocampal dentate gyrus of model rats.Results　 The abilities in spatial learning and memory， the numbers of BrdU-positive, BrdU+NF-positive and BrdU+GFAP-positive cells in encephalopathy group rats were significantly decreased(P<0.01), while insulin administration raised the above parameters near the normal levels of the control group rats (P<0.01). However, the migration of neural stem cells in the dentate gyrus was not affected even under the condition suffering from diabetic encephalopathy.Conclusion　 The decreases in cell survival rate and cell differentiation rate induced by a long-termed lack of insulin may result in the development of type 1 diabetic encephalopathy, indicating that early insulin replacement therapy can reverse the impairments in the dentate gyrus neurogenesis, and can help to prevent and to treat diabetic encephalopathy.]]> 2018/12/12 16:20:04 CUI Wei-gang, GUO Ling, LAN Ling, et al. 1004true ［Abstract］　Objective　To find out the clinical characteristics of female peptic ulcer disease.Methods　 The analysis of 6037 cases of female peptic ulcer from 17 hospitals in Nanning during 1992～1997 were performed.Results　 (1) Among all the cases, 5308(84.16%)cases were duodenal ulcers, 590(9.35%) cases were gastric ulcer, 134(2.22%)cases were compound ulcers, 5(0.08%)cases were esophageal ulcer.(2)The total sites of ulcer were 7187 sites, of them 6271(87.25%)sites were found in duodenal, 911(12.68%) sites were found in stomach, 5(0.07%) sites were found in esophagus. (3)Gastric ulcers were mainly found in gastric antrum(440 sites, 48.30%), duodenal ulcers were mainly found in front wall of duodenum(3082 sistes 49.14%), frost shape ulcer were mainly found in posterior duodenal bulb.(4)The majority of peptic ulcer diseases were found between the ages of 20 and 59 years(86.8%). With age increasing, the tendency of duodenal ulcer was decreasing and compound ulcer was increased.(5)The number of ulcer sites found in winter was the biggest, the next was in spring, the third was in autumn, the fourth was in summer. Conclusion　 Duodenal ulcer is the most commom in female ulcers, duodenal ulcers is most commonly found in bulb. Frost shape ulcer is most commmonly found in posterior duodenal bulb. The majority of peptic ulcer diseases are found in youth and middle age people, peptic ulcer diseases in winter and spring were more than in autumn and summer.]]> 2018/12/12 0:00:00 SUN Li-ling, HUANG Gang-ding, ZEN Guo-qun, et al. 1003true ［Abstract］　Objective　To detect the apoptosis of liver cell before and after hepatic ischemia-reperfusion, and explore the relationship between the liver ischemia-reperfusion of the clinical operation and apoptosis, in order to provide a theoretical basis for better preventing the hepatic ischemia reperfusion injury (HIRI) caused by the operation.Methods　 The apoptosis determination (TUNEL method) was used to measure the apoptosis index(AI) of hepatic cells before and after liver ischemia reperfusion. Results　Compared the AI of hepatic cells before Pringle′s maneuveur, at the time of the open of porta hepatis and before the closure of abdominal cavity respectively, differences among these three groups were significant (P<0.01). The AI of liver cells before Pringle′s maneuveur was higher than that at the open of porta hepatis (P<0.01), and also was higher than that before the abdominal closure (P<0.01), AI of liver cells at the time of the open of porta hepatis was higher than the AI before the abdominal closure (P<0.01).Conclusion　In the early of hepatic reperfusion injury after the short (about 15mins) term ischemia, apoptosis and ischemic reperfusion injury is a negative correlation, apoptosis is not the major way in the early injury of the liver cell.]]> 2018/12/12 16:20:04 WU Ji-hua, WEN Zhang, DUAN Jia-jia, et al. 1002true ［Abstract］　Objective　To select a suitable protein chip for detection of serum protein fingerprint from two kinds of chips(CM10 and IMAC3-Cu). Methods　 Serum samples from 13 patients with chronic hepatitis B, 13 patients with liver cirrhosis and 13 normal control people were collected respectively, peptide spectra were determined by SELDI-TOF-MS measurement. Results　 In the range from 2000Da to 50000Da, the number of significant protein peaks captured in each experimental group has a significant difference between CM10 and IMAC3-Cu chips（P<0.05）.Conclusion　CM10 chips can detect more significant difference protein peaks compared with IMAC3-Cu chips and preferably apply to the research of the serum protein fingerprints.]]> 2018/12/12 16:20:04 XU Ming-hui, DENG Jing-huan, LI Shan, et al. 1001true ［Abstract］　Objective　 To investigate the effect of acute hypervolemic hemodilution (AHH) with Gelofusine and 6%HES130/0.4 on serum with electrolytes and plasma histamine of cancer patients.Methods　 Sixty cancer patients of elective surgery esophageal cancer or colon cancer were randomly divided into two groups with 30 in each group. Group G received infusion of succinylated gelatin 15ml/kg; Group H received infusion of 6% HES 130/0.4 15 ml /kg. After induced anesthesia for 10 minutes, AHH were started in tow groups, venous blood samples before AHH (T₀), 5min after beginnig AHH (T₁), 30 min after beginnig AHH (T₂), 30 min after finishing AHH (T₃), 1hour after finishing AHH (T₄), 3 hours after finishing AHH (T₅)were collected for determining Hct, serum electrolyte Na⁺, K⁺, Ca⁺, Cl^- and plasma histamine. Results　(1)In inter-group comparision on serum electrolyte: at T₀ to T₅ there were not significant differences between group G and H. In intra-group　comparision there was rise trend of Na⁺ and Cl^-.　In intra-group comparision on serum electrolyte, at T₀ and T₁ to　T₅ there were significantly differences compareed with that at T₀ in two groups(P<0.05), and K⁺　and Ca⁺ decrease significantly(P<0.05). (2)In inter-group comparision on histamine: at T₀ to T₅ there were not significant differences between group　G and H. In intra-group comparision on histamine: in group G and H, the histamine at T₁ to T₅ were significantly higher than that at T₀(P<0.05). Conclusion　 Succinylated gelatin and hydroxyethyl starch in the implementation of mild AHH, have a certain influence upon serum electrolyte, however, no clinical symptoms, plasma histamine can slightly increased, but still within the normal range, do not cause symptoms.So they are safe in application of AHH.]]> 2018/12/12 0:00:00 LI Wen-mei, ZHANG Shu, SHI Hai-yan 1000true ［Abstract］　Objective　 To observe the effect and feasibility of endovaseular stent-assisted angioplasty to treat brain artery stenosis. Methods　Endovaseular stent-assisted angioplasty was performed in 33 patients with brain artery stenosis，including 14 cases of extracranial ternal carotid artery stenosis，3 cases of intracranial internal carotid artery stenosis，3 cases of middle cerebral artery stenosis，13 cases of extracranial vertebral artery stenosis, 2 cases of intracranial vertebral artery stenosis and 2 cases of subclavian artery stenosis. Results　 Among 33 patients, 35 of 37 stents were implanted successfully (94.59%). The complications include angiospasm, radiography medicament allergy and hyperperfusion syndrome in 3 patiens.Conclusion　 Endovaseular stent-assisted angioplasty is a effective methed to treat intraeranial artery stenosis，however the long-term effect needs to evaluate by flow-up.]]> 2018/12/12 16:20:04 ZHONG Wei-zhang, CHEN Yuan, XIAO Ji-dong， et al. 999true ［Abstract］　Objective　To compare the capabilities of entropy versus bispectral index values reflecting the stimulation of injury.Methods　20 consenting ASA physical status I and II patients scheduled for elective surgery under general anesthesia were evaluated. Both the BIS sensor and entropy sensor electrode strips were simultaneously applied to the patient′s forehead. Anesthesia was induced with propofol 2mg/kg, fentanyl 4μg/kg and atracurium 0.6mg/kg. Sevoflurane 3% (initial inspired concentration) in combination with intermittent bolus doses of atracurium (0.1mg/kg) were administered for maintenance of anesthesia. At the time-point of baseline, loss of consciousness, before, immediately, and 1min to 5min after intubation, the values of RE, SE, BIS, HR, MAP and SpO₂ were recorded.Results　The BIS, RE, and SE values decreased progressively from preinduction (baseline) values of 97±1, 89±2 and 96±4 to 5 min after intubation values of 41±10, 40±10 and 41±9, respectively. Compared to the values of before intubation, RE, SE and BIS of immediately at intubation were significantly increased, especially the RE value (P<0.01). MAP at the time-point of 1min after intubation were significantly increased compared to the value before intubation(P<0.01). HR at the time-point of immediately and 1min after intubation were significantly increased compared to the value before intubation(P<0.01).Conclusion　Entropy and BIS indexes and homodynamic indictors (MAP and HR) can reflect promptly the stimulation of tracheal intubation, especially the RE value.]]> 2018/12/12 16:20:31 CHEN Jun-xing, WU Ya-wen, SU Zhi-yuan, et al. 998true ［Abstract］　Objective　To explore the association of the HLA-DQA1 alleles with the different chronic hepatitis B of the Zhuang nationality in Guangxi.Methods　 In thirty patients with mild 、moderate and severe chronic hepatitis B in Zhuang nationality in Guangxi the HLA-DQA1 alleles were identified by using the polymerase chain reaction-sequence specific primers(PCR-ssp) technique.Results　The alleles gene frequencies of HLA-DQA1^*0103 in the Zhuang patients with mild and moderate chronic hepatitis B was singnificantly higher than that in the patients with severe chronic hepatitis B;HLA-DQA1^*0301 in the patients with severe chronic hepatitis B was lower than that in the patients with mild and moderate′s chronic hepatitis B.Conclusion　So DQA1^*0301 may be protective alleles of severe chronic hepatitis B in Zhuang nationality.]]> 2018/12/12 16:20:31 CHEN Zhi-ping, WEI Kang-lai，LI Ruo-lin, et al. 997true ［Abstract］　Objective　To evaluate the effect of dendritic cells(DC) sensitized by Exosomes in the treatment of advanced colorectal cancer.Methods　One hundred and five patients with advanced colorectal cancer were randomly divided into combination treatment group (chemotherapy +DC sensitized by Exosomes) (n=65) and chemotherapy group(n=50) for observation of the T cell subsets in peripheral blood, response rate, one year survival rate, median survival time and toxicity.Results　 CD3⁺、CD4⁺ and CD8⁺ in combination treatment group after treatment were improved（P<0.05）, which was also superior to chemotherapy group（P<0.05）. Response rates were 56.9% in combination treatment group and 38.0% in chemotherapy group（P<0.05）. One year survival rate and median survival time were 66.7% and 15.6 months, respectively, in patients allocated to combination treatment group compared with 52.1% and 12.3 months, respectively, in patients allocated to chemotherapy group（P<0.05 for both comparisons）. There was no significant difference in toxicity between both groups（P>0.05）. Conclusion　Dendritic cells sensitized by Exosomes combined chemotherapy is well effective and tolerated in the treatment of advanced colorectal cancer, improve immunity function and prolong survival period.]]> 2018/12/12 0:00:00 HUANG Shi-feng, LIU Bin, DAI Sheng-ming, et al. 996true ［Abstract］　Objective　To investigate the characteristics of wavefront aberration in different myopes.Methods　One hundred and five myopic out-patients (male 54, female 51) between March and July 2008 in Guangxi visual science and optometry center, aged from sixteen to thirty six, and refractive power ranged from -0.50 to -10.50 diopters(spherical equivalent) were enrolled retrospectively. And the myopes were divided into low, moderate and high groups. WaveScan^® system(Visx corp. made in USA) was used to capture each myopic wavefront aberration images. Root mean square wavefront error was used as a parameter of myopic optical quality. One-way ANOVA and LSD were made to compare to wavefront aberration differences among the three groups by SPSS 13.0 version software.Results　LSD analysis showed that there were significant differences among the three groups in total aberrations (F=203.044, P<0.01), and the higher of myopia, the larger of total aberrations. But total higher-order aberration, trefoil, coma and spherical aberration didn′t show significant difference among the three groups(P>0.05). And these four indices demonstrated a descending trend orderly in each group.Conclusion　 Myopic total aberration would be increased with the growth of myopia, but higher-order aberrations just shew individual differences among the myopes in different levels.]]> 2018/12/12 0:00:00 HUANG Jian-zhong, ZHAO Wu-xiao, LIU Wei-min, et al. 995true ［Abstract］　Objective　To study the stability of compound pueraria lobata capsules.Methods　The compound pueraria lobata capsules was stored in room temperature.At the time of 0, 1, 2, 3, 6 and 12 month, the appearance and properties of compound pueraria lobata capsules were observed, the panax quinquefolium L was identified by TCL identification method，and the content of puerarin was determined by high performance liquid chromatography.Results　The results of appearance, properties, identification, determination of three groups of preparation were qualified.Conclusion　According to the standard, the stability of compound pueraria lobata capsules is qualified.]]> 2018/12/12 16:20:31 WEI Hua-ling, YANG Jia-zhen，PAN Hong-ping. 994true ［Abstract］　Objective　To study the technique and clinical effects of ultrasound　capsulotomy instrument in senile cataract surgery.Methods　 Ninety patients of senile cataract surgery underwent continuous circular capsulorhexis with hand-made methods (43 cases) and ultrasound　capsulotomy instrument (47 cases), and the surgery process was compared.Results　Compared with hand-made group, the time of capsulorhexis in the group of continuous circular capsulorhexis with ultrasound　capsulotomy instrument was shorter(P<0.01) in senile cataract surgery.Conclusion　The capsulorhexis with ultrasound　capsulotomy instrument is relatively easily, quickly and safe controlled in senile cataract surgery.]]> 2018/12/13 9:29:22 ZENG Si-ming, LIN Yu, LI Li, et al. 993true ［Abstract］　Objective　To study the sexual difference and intervention methods of psychological problems existed in middle school students.Methods　The students who were from different grade of two middle schools were randomly divided into two groups, interventional group and control group. The sexual difference of psychological problems between male and female students were investigated and practiced different intervention on different problems, and then all the students were tested by the symptom self-evaluation form (SCL-90) before and after intervention.Results　After intervention, the discovery rate of psychological problems in interventional group and control group was 9.6% (22/230) and 16.1% (39/242) respectively, which had statistical significance(P<0.05). Before intervention, the discovery rate of depression, anxiety and phobia among female students was significantly higher than that of male students (P<0.05), on the other hand the discovery rate of hostility and paranoia among male students was significant higher than that of female students (P<0.05). Except depression, the discovery rate of all the symptom mentioned above decreased after intervention and there was no statistical significance of the difference between male and female students.Conclusion　 In order to improve the psychological health conditioni in middle school students, it is necessary for us to pay attention to the sexual difference of psychological problems and practise early comprehensive intervention.]]> 2018/12/13 9:29:22 HE Hai-ying, WANG Ling, WANG Mei, et al. 992true ［Abstract］　Objective　To find a ideal cover material for repairing of deep-burned functional part and scar deformity.Methods　 The scar or wound of the deep-burned functional part were excised, and then the lesion were covered with acellular allograft derma plus autogenous split-thickness skin graft.Results　twenty-five cases were included in this study. The survival rate of the composite skin grafts was 100%. All the composite skin grafts were smooth and soft, had good elasticity, and the joint functions were good.Conclusion　 Acellular allograft derma with autogenous split-thickness skin graft is an ideal cover material in wound repair of functional part.]]> 2018/12/13 9:29:22 QIN Qiu-hai, HUANG Yun-yan, BIAN Jian-min, et al. 991true ［Abstract］　Objective　 To report the clinical effect of replantation by anastomosing only the branches of the terminal digital arterial arch.Methods　Twenty-four fingertips amputated in the zone distal to the middle nail and without anastomosable vein were replanted. By anastomosed only the branches of the terminal digital arterial arch, and without adopted postoperative bloodletting.Results　 Twenty-two replanted fingertips survived, the survival rate was 91.6%.During the follow-up 6 months to 24 months, the 22 replanted fingertips survived with satisfactory shape and function， the sensation of discrimination between two points was 4～7 mm. Conclusion　The curative effect of replantation of the amputated fingertips distal to middle nail with anastomosing only the branches of the terminal digital arterial arch was satisfactory.]]> 2018/12/13 0:00:00 LIAO Jian-wen, ZHANG Zhen-wei, CHEN Ze-hua, et al. 990true ［Abstract］　Objective　To explore the effect of the extracting solution from mutouhui on the microvessel-density and VEGF/bFGF expression in the U14 replanted tumor tissues of the mice that had cervical carcinoma.Methods　The SPF female mice were divided into high dose/ middle dose/ light dose of the extracting solution group, cisplatin group and model group randomly.Then the effect of the extracting solution from mutouhui on the microvessel-density and VEGF/bFGF expression in the U14 replanted tumor tissues of the mice that had cervical carcinoma were observed.Results　The microvessel-density in high dose/ middle dose of extracting solution group and cisplatin group was lower than that in model group; VEGF expression in high dose/ middle dose of extracting solution group and cisplatin group was lower than that in model group; bFGF expression in high dose/ middle dose of extracting solution group and cisplatin group was lower than that in model group. Conclusion　The extracting solution from mutouhui is against the formation of the microvessel-density in U14 transplanted tumor, and it also can reduce the VEGF/bFGF expression, all these show that extracting solution from mutouhui has the effect of anti-tumor.]]> 2018/12/13 9:29:50 JIANG Qiu-yan, FANG Gang, SU Li-ming, et al. 989true ［Abstract］　Objective　To enhance the knowledge of pulmonary nocardiosis which is an infrequent infectious disease and usually misdiagnosed during clinical practice.Methods　The clinical data of 4 patients with pulmonary nocardiosis were analyzed and related literatures were reviewed. Results　Two of 4 patients with pulmonary nocardiosis were suffering from autoimmune diseases and were receiving corticosteroid therapy. All of the patients had productive cough and fever, of them 3 patients had afternoon or night fever. The radiographic picture included multilobar consolidation (4/4), pleural effusion (4/4) and multiple cavitary masses (3/4). The patients were good responsive to trimethoprim-sulfamethoxazole or combination of the third generation cephalosporins. Conclusion　The clinical data of pulmonary nocardiosis are non-specific. Multiple cavitary masses are the predominant radiological patterns. Cotrimoxazole is the most commonly used empirical treatment.]]> 2018/12/13 9:29:50 QIN Zhi-qiang, LV Jing, LONG Sheng-ze 988true ［Abstract］　Objective　To analyze the oral dental behaviors of kids of 0～3 years′ old in Nanning Guangxi, on the basis of the results of the parents questionaire on life-styles and eating habits of kids.Methods　Two hundred and fifty-six kids of 0～3 years old were randomly chosen from those who came to the Preventive Health Branch in our hospital.Their parents were investigated with questionnaire. The resulting data were processed by the statistic software SPSS11.0.Results　The results showed there were no significant difference between Han and minority nationalities in kids′ dental behaviors including kids′ feeding methods, eating habits, life-style, oral health knowledge and preventive solutions of parents(P>0.05).Conclusion　These kids of 0～3 years′ old in Nanning Guangxi have built up decent life-style. After systematic and intentional oral prevention, it is possible to achieve better results.]]> 2018/12/13 9:29:50 SHI Chun-mei, ZHOU Yan, LI Yan, et al. 987true ［Abstract］　Objective　To detect surface receptors mRNA expression of integrins in pulmonary thromboembolism, to analyze the significance of its differential expression.Methods　Twenty patients with PTE were enrolled in our study and the other twenty patients without PTE were allocated as controls.Total RNA was abstracted from isolated mononuclear cells in peripheral blood samples of twenty patients and controls.Significant differential mRNA expression of integrins receptors were analyzed by means of mRNA expression microarray.Results　Fourteen pieces of mRNA fragments associated with leucocyte were screened and part of the genes were up-regulated in PTE group compared with control group (P<0.05).The up-regulated mRNA fragments associated with leucocyte were mainly groups β1 and β2.Eleven pieces of mRNA fragments associated with platelet were screened and 60% of the genes were up-regulated in PTE group compared with control group (P<0.05).The up-regulated mRNA fragments associated with platelet were mainly groups β1 and β3.Eleven pieces of other mRNA fragments were screened and part of the genes were up-regulated or down-regulated, of which ligands were mainly fibronectin.Conclusion　A great part of integrins associated with leucocyte and platelet were up-regulated in PTE, and the integrins whose main ligand being fibronectin were also significantly different between PTE group and control group.Inflammation and platelet aggregation which was induced by them may be one important pathological mechanism of PTE.]]> 2018/10/22 8:14:38 XIE Yuan, GONG Zhu, WANG Le-min 986true ［Abstract］　Objective　To observe the effect of hypoxia and high temperature on the microcirculation in the state of myocardial ischemia in rabbits.Methods　Twenty rabbits were randomly divided into the myocardial ischemia groups and the hypoxia and high temperature of myocardial ischemia groups, after myocardial ischemia, the hypoxia and high temperature of myocardial ischemia group were put into a hypoxia and high temperature state (42±0.5)℃, and inbreathed a mixture gas made of 8.5% oxygen and 91.5% nitrogen), after 30 min, the rabbits were taken out of, and the changes of microcirculation of mesentery were observed. The rabbits of myocardial ischemia groups were taken out of room temperature (25℃ with 30 min), and compared with the hypoxia and high temperature of myocardial ischemia group.Results　In the myocardial ischemia group the microcirculation of mesentery in rabbits did not change significantly （P>0.05）.But in the hypoxia and high temperature of myocardial ischemia group mesenteric microvessel expanded（P<0.05） and the speed of blood stream slowed down （P<0.01）.Conclusion　After myocardial ischemia, hypoxia and high temperature can obviously change the microcirculation of mesentery in rabbits.]]> 2018/10/22 8:14:38 ZHAO Shan-min, HE Xian-jiao, HUANG Li-juan, et al. 985true ［Abstract］　Objective　To explore the personality characteristics of high school students of different nationalities who live in same region.Methods　One hundred and seventy-two high school students were administered EPQ.Results　It showed that P scores of EPQ in male students were higher than in female students. But N scores in male students were lower than in female students significantly. Compared with EPQ norms of China, P scores and L scores in male and female students were lower. N scores and E scores in female students were higher. There was no difference in each score of EPQ between different nationality students.Conclusion　The high school students of different nationality who live in same region have similar personality characteristics.]]> 2018/10/22 8:14:38 LU Xue-ping 984true ［Abstract］　Objective　To assess the value of nuchalskinfold thickness (NT) by ultrasonic B in screening Down syndrome at early and middle stage pregnancy.Methods　Nuchalskinfold thickness (NT) of 1500 pregnant women (12～27weeks) were detected by ultrasonic B, and results were compared with chromosomal karyotypes analysis and follow-up visit of each pregnant woman.Results　In 1 500 cases incidence of Down syndrome was 3.3‰, and 62 cases were high-risk for NT by ultrasonic B and including 4 Down syndrome cases;in low-risk for NT cases, 1 Down syndrome case were found. The NT sensitivity to Down syndrome was 80%, specificity was 96.07%, accuracy was 96.12% and the positive predictive rate was 6.45%.Conclusion　Detection of NT by ultrasonic B may play an important role in screening Down syndrome at early and middle stage pregnancy.]]> 2018/10/22 8:14:38 MO Wei-ying, WAN Li-kai, CHEN Yan-hong 983true ［Abstract］　Objective　To study the influential factors of the β₂-MG、NAG and RBP evaluating the nephrotoxicity caused by lead.Methods　In the study, the methods of the correlation analysis and group comparison were adopted.Results　The data of the β₂-MG, NAG and RBP are related to the age and receptive-lead standing (r: 0.221、0.230、0.433，0.359、0.419、0.365).And the data of β₂-MG and RBP related to the PbB (r: 0.419、0.354).Moreover, the relations among the β₂-MG、NAG and RBP were existing.Conclusion　When the β₂-MG, NAG and RBP are used to evaluate the nephrotoxicity caused by lead, the indexes are influenced by the age, receptive-lead standing and PbB.]]> 2018/10/22 8:14:38 SU Su-hua, JIANG Dong-fang, NON Kang, et al. 982true ［Abstract］　Objective　To survey the physical and mental health of communications system division-level leading cadres in Guangxi, propose a solution to improve the health status of cadres.Methods　Using self-compiled questionnaire with SCL-90 and other commonly used psychometric instruments to check the 227-job level leading cadres.Results　Leading cadres′ physical and mental health is alarming: “sub-healthy” is widespread;SCL-90 measurements showed that mental factor scores were significantly higher than the national norm for adults;the physical health status, social support and personality characteristics of leading cadres are closely related to their mental health.Conclusion　Strengthen the health education of cadres;vigorously promote national fitness campaign, to build a platform for mental health maintenance for leading cadres by taking various measures and through muti-scetoral cooperation.]]> 2018/10/22 8:14:38 JIANG Min, LU Yun, CHENG Zhong-hui, et al. 981true ［Abstract］　Objective　To investigate the variation of myopic wavefront aberrations after conventional LASIK. Methods　Thirty five myopic out-patients (male 18, female 17) underwent conventional LASIK between March and July 2008 in Guangxi vision science and optometry center, aged from sixteen to thirty six, and refractive power ranged from -0.50 to -10.50 diopters(spherical equivalent) were enrolled retrospectively. WaveScan^ system(Visx corp. made in USA) was used to capture each myopic wavefront aberration images at pre- and post- 1 day, 1 week, 1 month, 3 month and 6 month after conventional LASIK, respectively. Root mean square of wavefront error was used as a parameter of myopic optical quality in this study. One-way ANOVA and Game-Howell and LSD were analyzed to compare to each type of wavefront aberrations among the six time points by SPSS 13.0 version software.Results　Total aberration:(1)Significant differences were showed between pre- and post-LASIK(all P=0.000);(2)but there were no differences among the different time points after LASIK(P>0.05). Coma:(1)There were no differences neither between pre- and post-LASIK at day one, nor among the five time points after LASIK except at day one;(2)but there were statistic differences either compared pre-LASIK or compared post- 1 day with other time points after LASIK(P<0.05). Spherical aberration:(1)There were no differences when compared pre-LASIK with post- 1 day, 1 week or six month after LASIK. And there were no differences among the different time points after LASIK except post- 1 day(P>0.05);(2)There were significant differences when compared pre-LASIK with post- 1 month or 3 month, and compared post- 1 day with any other time points, too(P<0.05). No statistic differences were showed both total high order aberrations and trefoil aberration among the six different time points(P>0.05).Conclusion　Myopic total aberration maitained relatively stable, but spheric aberration and coma varied at early period after conventional LASIK.]]> 2018/10/22 8:14:38 ZHAO Wu-xiao, HUANG Jian-zhong, LIU Wei-min, et al. 980true ［Abstract］　Objective　To compare the efficacy of non-steroidal drugs (NSAIDs), hyaluronic acid (HA), bone marrow mesenchymal stem cells (BMSCs) for knee osteoarthritis of various periods.Methods　Sixty patients with knee osteoarthritis confirmed by arthroscopic operation were randomly divided into 3 groups : (1)NSAIDs group was treated with NSAIDs for 2～4 weeks after operation;(2)HA group was treated by injection of HA 1～2 courses after operation;(3)BMSCs group was treated by intra-articalar replantation of BMSCs obtained by means of isolation and culture of iliac crest bone marrow. The knee joint function were evaluated by WOMAC index scores, 3 months, 1 year, 3 years after surgery.Pairwise comparison between the three groups were performed.Results　3 months follow-up showed the treatment results of three groups was not statistically difference; 1 year follow-up showed in comparison of HA group and the BMSCs group with the NSAIDs the treatment results were statistically difference; 3-year follow-up showed three groups were statistically difference between the treatment effect.Conclusion　The early efficacy of three kinds of methods for osteoarthritis are good, but the long-term effects of BMSCs group is superior to the other 2 methods.]]> 2018/10/22 8:14:38 XU You-gao, HU Jv-zheng, WANG Yong, et al. 979true ［Abstract］　Objective　To summarize the perioperative complication and its treatment of off-pump coronary artery bypass grafting (OPCABG). Methods　 From June 2001 to May 2009, among 121 patients with OPCABG., 63 (A group) patients were treated by the first use of grafts with sequential proximal-type methods of coronary artery bypass graft(CABG);Another 58 (B group) patients were treated by the first coronary artery target vessel with a remote client approach.Results　In two groups the perioperative complication was found in 49 patients. The complication rate in A group was lower than that in B group.Of 121 patients, 118 patients were cured and 3 died. The cause of death was perioperative myocardial infarction in 1 case; Acute renal failure in 1 case; Sternum-mediastinal severe infection in 1 case.Conclusion　In OPCABG, the occurrence of postoperative arrhythmia is still inevitable. Strengthening the perioperative management can reduce the complication.]]> 2018/10/22 8:14:38 WEN Zhao-ke, LIN Hui, LI Xiang-wei, et al. 978true ［Abstract］　Objective　To investigate the differnces of two types of corneal topography system′s outcome in myopes.Methods　Forty-five myoipc out-patients (male 25, female 20) of Guangxi vision science and optometry center in Feb. 6, 2009, aged from nineteen to thirty-nine, and refractive power ranged from -0.50 to -9.00 diopters(spherical equivalent) were enrolled in this study. Orbscan-Ⅱz (Bause-Lomb corp. made in USA) and Humphrey ALTAS (Zeiss corp. made in Germany) corneal topography system were used to capture each myopic corneal images, respectively. And anterior corneal surface indexes such as Sim K′s， Axis， Max.， Min. values were obtained in this study. Paired t-test were used to compare the differences of two corneal topography systems by SPSS 13.0 version software. Results　Outcomes demonstrated that Sim K′s， corneal astigmatism axis， Max.， and Min. values were all different in this study. Paired t-test showed that P values were 0.037、 0.004、 0.072、 0.000, respectively. Max.， Min. values and corneal astigmatism axis in Humphrey ALTAS were greater than those in Orbscan-Ⅱz corneal topography system. But Sim K′s values in Humphrey ALTAS were less than that in Orbscan-Ⅱz corneal topography system. Conclusion　Fine agreements were found in Sim K′s， Axis， Max， and Min values between Orbscan-Ⅱz and Humphrey ALTAS corneal topography system.]]> 2018/10/22 8:14:38 LI Xiao-bo, LIU Wei-min 977true ［Abstract］　Objective　To study the effect of trans-intra-iliac arterial prostatic arterial chemo- embolization in the treatment of advanced prostate cancer. Methods　The therapy was performed in 22 patients with advanced prostate cancer for 25 times. Five cases were in stage C1; 7 in stage C2; 8 in stage D1; 2 in stage D2. The drug dose was CBP 400 mg, MMC 20 mg, epirubicin 50 mg, VCR 1.0 mg and the embolic agent was gelfoam. Results　After one week of the therapy, the symptoms of all the patients were relieved and their prostates got softened and regressed. After 2～3weeks of the therapy, the regression rate was 20%～40%. The neuro-complications occurred in 3 cases. And 8 cases were treated with urethroelectrotomy after embolization. The follow up time was 5～36 months. Results showed their urinary fllow rate was improved and residual urine volume diminished or eliminated. Conclusion　Transcathter bilateral intra-iliac superselective chemo-embolization has a positive effect on advanced prostate cancer. It has provided a new method for the comprehensive treatment of advanced prostate cancer, especially for those which belong to androgenic-hormone dependent type, or those which are non-sensetive to the endocrine treatment.]]> 2018/10/22 8:14:38 HUANG Heng-hai, XUE Yuan-ling, HUANG Zhi-hong, et al. 976true ［Abstract］　Objective　To find the model that care the emotions of the patient with logaphasia.Methods　Sixty-two patients with logaphasia were randomly prospectively divided into two groups and treated with multipath nervous stimulating therapy and basic therapy.Results　Multipath nervous stimulating therapy can improve the emotions of the patient with logaphasia.Conclusion　The patients with logaphasia are very painful in the mind and be neglected seriously, multipath nervous stimulating therapy help them improve the emotions and make them feel happy.]]> 2018/10/22 8:14:38 KUANG JIn-bo, SU Xiou-qing, HUANG Run-jian, et al. 975true ［Abstract］　Objective　To prospectively probe into the clinical effect of long-term anti-tuberculosis drugs in the treatment of long-term mycobacterium tuberculosis-carrying silicosis patients complicated with tuberculosis. Methods　Twenty-four cases of long-standing mycobacterium tuberculosis silicosis complicated with tuberculosis were randomly divided into two groups： observation group and control group. The former included 11 cases with taking anti-tuberculosis drugs in a long term; the latter included 13 cases with clinical follow up but without taking anti-tuberculosis drugs in a long term. Results　Survival time with mycobacterium tuberculosis： 5～18 living years in the observation group, average 12.72 years, 3～6 years in the control group, average 3.85 years, the comparison between the two groups had a very significant difference (P<0.01). Infectious case of tuberculosis： 6 cases in the observation group, 52 cases in the control group, the comparison between the two groups had a very significant difference (P <0.01); Survival life time： the observation group was 63～82 years old， average 73.19 years old， the control group was 59～74 years old, average 63.92 years old, the comparison between the two groups had a significant difference (P<0.05). Conclusion　Long term taking the anti-tuberculosis medicines is one of the effective ways in the treatment of long-term mycobacterium tuberculosis-carrying silicosis patients complicated with pulmonary tuberculosis, It may lengthen the patient′s survival time with mycobacterium tuberculosis and survival life effectively, and reduce tuberculosis infectious case to occur, It should be used as one of the effective prevention and control measures to long-standing mycobacterium tuberculosis silicosis complicated with tuberculosis.]]> 2018/10/22 8:15:02 HUANG Gui-ping, QIU Li 974true ［Abstract］　Objective　To investigate the etiology, prognosis, risks factors of death among acute renal failure (ARF) patients，and provide a theory basis for the prevention and treatment of ARF.Methods　ARF patients admitted in First Affiliated Hospital，Guangxi Medical University from January 1st 2002 to December 31st 2006 were analyzed retrospectively, according to the etiology, clinical features, prognosis.Results　There were 193 038 admissions during the study period and 611 met the diagnostic criteria of ARF.The overall incidence of ARF was 0.32%. The ratio of male：female of ARF was 1.87∶1.00 with median age 40 years o1d. Among 611 patients, 118(19.31%) were pre-renal ARF, the first cause of pre-renal ARF was low cardiac output(35 cases， 29.66%）.Three hundred and seventy-two(60.88%) patients were renal parenchymal ARF, the main cause of parenchymal ARF was tubulointerstitial injures(236 cases); of them 95 cases were caused by drugs. One hundred and twenty-one (19.80%) patients were post-renal ARF, the main cause of post-renal ARF was urinary stone disease(84/121). Two hundred and one(32.90%) patients were acute-on-chronic renal failure(A/C). The mortality was 14.89% in patient with ARF.The results of logistic regression analysis suggested the oliguria, MODS and HA-ARF were the risk factors of death among ARF patients.Conclusion　Low cardiac output, drugs, urinary stone disease were the main cause of pre-renal ARF, parenchymal ARF, post-renal ARF respectively.A/C was an common part of ARF. The mortality of ARF patients with oliguria, MODS and HA-ARF was higher.]]> 2018/10/22 8:15:02 HUANG Li, XU Jin, LIAO Yun-hua 973true ［Abstract］　Objective　To explore the relationship of sports fatigue of athlete and the change of picture of the tongue.Methods　The picture of the tongue and the self-sensation fatigue of 125 special athletes after different exercise intensity were observed, The table of self-sensation was finished by athlete and the table of picture of the tongue was finished by doctor of traditional Chinese medicine.Results　In 125 athletes, 96 athletes had self-sensation fatigue(76.8%) after winter exercise, 76 athletes had thin yellow lingual fur picture of the tongue（60.8%）, 31 athletes had thin white lingual fur picture of the tongue（24.8%）, 12 athletes had yellow thick fur picture of the tongue（9.6%）. 68 athletes had light red tongue（54.4%）, 18 athletes had red picture of the tongue（14.4%）, 11 athletes had crack picture of the tongue（8.8%）, 9 athletes had indentation picture of the tongue（7.2%）, 6 athletes had magnum picture of the tongue（4.8%）, 3 athletes had lean yellow picture of the tongue（2.4%）. Conclusion　The picture of the tongue will change regulary after exercise fatigue which also can be felt by athletes, which can be used to evaluate the sports fatigue.]]> 2018/10/22 8:15:02 DU Jian-xiong, YANG Xiao-ying, LIU Yan, et al. 972true ［Abstract］　Objective　To discuss ideal prosthetic method of front teeth in change direction.Methods　Fifty-two front teeth being trusion or protrusion were restored with everStick fiber posts and full baked porcelain crown, and followed up 1 to 2 years.Results　All crows were sealed and have good retentive power, without crown or root fracture.Conclusion　The restoration of front teeth can be improved by aesthetics and function of everStick fiber posts and all-ceramic crown.]]> 2018/10/22 8:15:02 PENG li-hui, ZHONG Ai-xi, LIN Guang-xue, et al. 971true ［Abstract］　Objective　To investigate the relationship between the expression of the vascular endothelial cell growth factor(VEGF) and KDR and microvessel density(MVD) in hemangioma.Methods　Immunohistochemical streptavidin/peroxidase(SP)stain was applied to examine the expression of KDR、VEGF and CD34.The amount of microvessel was counted by the expression CD34 in endothelial cell.Results　In 35 cases，positive rate for KDR was 73.4%，for VEGF was 66.5%.In the proliferating phase，positive rate for KDR was 89.3%, for VEGF was 92.1%.However，the positive rate for KDR, in the involuting phase, was 42.5%, and for VEGF was 31.4%. There was a very significantly difference between two phases(P<0.01). In the proliferating phase，MVD was (75.7±13.71). In the involuting phase, MVD was (28.9±8.5)(P<0.01).The correlation between the KDR and VEGF was positive(P<0.01). There was a positive correlation between the KDR and MVD(P<0.01).Conclusion　There might be a special interaction between the KDR and VEGF in endothelial cell of the hemangioma in proliferating phase，which could result in the angiogenesis.]]> 2018/10/22 8:15:02 LIANG Jie, HUANG Qi-jia 970true ［Abstract］　Objective　To compare the antiemetic efficacy of ondansetron, ondansetron combined omeprazole, ondansetron combined mild moxibustion, three kinds of programs to prevent chemotherapy induced gastrointestinal reactions. Methods　Ninety patients treated with cis-platinum-based chemotherapy were randomly divided into three groups of 30 cases, respectively. These programs were used to observe the effect of prevention of chemotherapy indaced digestive tract reaction.Results　For vomiting, three programs′efficiency were similar, respectively, 80%, 87%, 90%;For the nausea, three programs were effective, respectively, 63%, 80%, 90%, the difference was significant (P<0.05). For the loss of appetite, the effective rate of three programs was 17%, 53%, 80%, there are significant differences (P<0.005). Conclusion　Ondansetron alone can not completely prevent chemotherapy-induced gastrointestinal reaction, The efficacy was somewhat increased imder the condition of ondasetron combined with omerazole. Ondansetron combined with mild moxibustion is superior to ondansetron and ondansetron combined omeprazole, in particular for preventing the nausea and loss of appetite after chemotherapy.]]> 2018/10/22 8:15:02 HUANG Qiao-tong 969true ［Abstract］　Objective　To use ROC curve to choose the cut-off of PP value, CP value and VP value, and investigate the value of them in the diagnosis of Meniere′s disease（MD）. Methods　The MR hydrography images of 22 MD patients and 20 normal volunteers were analyzed retrospectly, the minimum distance between the posterior semicircular canal and the posterior petrous surface (PP value), between the common crus and the posterior petrous surface (CP value), and between the vestibule and the posterior petrous surface (VP value) were measured in axial plane, and ROC curve was established by them.Results　The area under ROC curve of PP value, CP value and VP value reached 0.814, 0.837 and 0.796, and the best diagnostic cut-off was 2.035 mm, 1.995 mm and 4.730 mm respectively, the sensitivity of them was 72.7%, 70.5% and 65.9%respectively, and specificity was 85.0%, 92.5% and 90.0%.Conclusion　PP value, CP value and VP value have medium value in diagnosis of MD, useing ROC curve can choose the best cut-off of them.]]> 2018/10/22 8:15:02 HUANG Yao-qu, ZHAO Xiao-mei, QIN Hong-wei, et al. 968true ［Abstract］　Objective　To observe retrospectively the expression and significance of nuclear BAG-1 in early breast cancer(EBC) and its relationship with clinical pathological features and ER, PR, HER2.Methods　A total of 76 patients with EBC were followed up in this study. The protein expressions of nuclear BAG-1, ER, PR and HER2 in these patients were detected by immunohistochemical two-step method and statistically analyszed.Results　The positive rate of protein expression of nuclear BAG-1 was 14.47%. The protein expression of nuclear BAG-1 was not related to age, menopausal status, histology and HER2(P>0.05), but positively related to PR and ER(P<0.05). Nuclear BAG-1、ER、HER2 were correlated with prognosis in univariate analysis with COX proportional hazard model. In multivariate analysis, only nuclear BAG-1 was correlated with prognosis.Conclusion　The results of this small pilot study suggest that the protein expression of nuclear BAG-1 in EBC was correlated with ER and PR, that might be a novel prognostic biomarker.]]> 2018/11/7 8:22:37 QIN Yu-zhou, OU Hai-ling, LIU Jian-lun 967true ［Abstract］　Objective　To investigate the clinical features of secretary otitis media(SOM) in a comparative way with that of SOM group and health adult control group, and analyze the correlation between hearing loss of SOM and age, duration.Methods　The 0.125～8 KHz air conduction(AC) audiometry and 0.25～4 KHz bone conduction(BC) audiometry were administered to 37 adult patients(47 ears)with SOM group and 25 control group (50 ears).Then their clinical data, especially that of pure tone audiometry, were analyzed in a comparative way between them. And observed the correlation of the auditory threshold with the age and the duration of patients.Results　There were 78.72% (37/47ears) conductive hearing loss, and 21.28% (10/47ears) mixed hearing loss was found in patients. AC threshold in both frequency ranges of SOM group were significant higher than that of control group (P<0.01). BC threshold of SOM group were significant higher than that of control group (P<0.01). Pure-tone average thresholds (PTA) and bone average thresholds and air-bone gap(ABG) of SOM group were significant higher than that of control group (P<0.01). It demonstrates a strong correlation between PTA, bone average thresholds, ABG and the age in SOM group (P<0.01).There was positive correlation between PTA and the duration in SOM group (P<0.05). There were not correlation between ABG and the duration in SOM group (P>0.05).Conclusion　The results suggest that SOM in adults may not only affect middle ear mechanics, but also impair inner ear. Age and duration are important influencial factors to auditory of SOM.]]> 2018/11/7 8:22:37 LU Qiu-tian, ZHU Qiu-mei, LIANG Jian-ping, et al. 966true ［Abstract］　Objective　To investigate the changes of heart rate variability (HRV) in hypertensive patient complicated with type2 diabetes mellitus (EH + T2DM) after accepting treatment of conventional drug and exercise prescription.Methods　One of exercise prescription was 15 minutes movement daily; the other exercise prescription was 30 minutes once every two days.Exercise target heart rate approximately equal to ｛（60%～70%）×［（220～170）-actual age）］｝ times/min. The 30 patients with abnormal HRV were divided into three groups，all were accepted conventional drug treatment. In addition, Group A accepted exercise prescription 1, Group B accepted exercise prescription 2, and group C did not accept the exercise prescription.Results　All abnormal HRV in these patients were all improved.Compared the results before treatment with those after treatment, the changes in HRV in group A and B were significant（P<0.05）, while that in the group C were not significant（P>0.05）. The changes in HRV between group A and B were no significant（P>0.05）.Conclusion　Conventional medical treatment can not obviously improve abnormal HRV in (EH + T2DM) patients; Moreover, the improvement will be more obvious while conventional drug therapy combined with exercise prescription were applied.]]> 2018/11/7 8:22:37 MO Yi, LIU Yu-tian 965true ［Abstract］　Objective　To explore the impact of medication self-management module on treatment compliance, social function and quality of life of patients with schizophrenia. Methods　Sixty-six inpatients with schizophrenia in non-acute stage were randomly divided into training group(n=33) and control group(n=33).Both groups received the anti-psychotics therapy. Medication self-management module was only given to the training group for 8 weeks. All patients were followed up for 6 months and were evaluated with self-made drug treatment compliance rating scale, social disability screening schedule(SDSS), generic quality of life inventory-74 (GQOLI-74). Results　Treatment compliance, the total scores of SDSS and the total scores of GQOLI-74 of training group were significantly higher than those of control group after 8 weeks and 6 months(P<0.01, P<0.05). Treatment compliance, the total scores of SDSS and the total scores of GQOLI-74 of training group after 8 weeks and 6 months were significantly higher than those of pretraining(P<0.05). Treatment compliance of both groups on 6 months follow up were significantly lower than that of 8 weeks (P<0.01).Correlation analysis confirmed negative relation between the total scores of GQOLI-74 and the total scores of SDSS, treatment compliance, and confirmed positive relation between the total scores of SDSS and treatment compliance on 8 weeks and 6 months（P<0.01).Conclusion　Medication self-management module could significantly improve treatment compliance, social function and quality of life of patients with schizophrenia.]]> 2018/11/7 8:22:37 ZHANG Ling, XIE Yan, HE Fen-lan, et al. 964true ［Abstract］　Objective　To investigate electrocardiogram changes of the patients with metastases of lung cancer by radiatherapy.Methods　From January 1999 to December 2007, 48 patients of multiple pulmonary metastases in our hospital were treated by whole-lung radiation therapy. The clinical data of these patients were retrospectictively analyzed.Results　The abnormal ECG rate was 31.3% (15/48), in which sinus tachycardia (>100 beats/min) accounted for 12.5% (6/48), sinus bradycardia (<60 beats/min) accounted for 8.3% (4/48), ST segment changes (ST segment depression more than 0.05mv,) accounted for 8.3%(4/ 48), complete right bundle branch block accounted for 2.1% (1/48). Abnormal ECG were appeared respectively in 2 cases at the time of the 3rd weeks, 5 cases at the 4th weeks in the treatment, 8 cases at the end of the treatment, All of the ECG resumed normal by follow-up for 3 months after the treatment.Conclusion　Multiple pulmonary metastasis can be treated by whole lung radiation therapy available to good effect. Radiation of large area with less radioactive exposure dose can cause rapid cardiac injury, the injury is mild and transient.]]> 2018/11/7 8:22:37 ZHENG Yue-hai，RAO Jian, FANG Hong, et al. 963true ［Abstract］　Objective　To investigate the variation of Q values after conventional LASIK.Methods　Twenty-five myopic conventional LASIK candidates (male 13, female 12) between March and July 2008 in Guangxi vision science and optometry center, aged from nineteen to thirty six, and refractive power ranged from -0.50 to -10.50 diopters(spherical equivalent) were enrolled retrospectively. Orbscan-Ⅱz corneal topography (Bause-Lomb corp. made in USA) was used to capture each myopic corneal images at pre- and post-1 week and 1 month after conventional LASIK, respectively. And Q values were harvested at each time point in this study. One-way ANOVA and Games-Howell were used to compare Q values among the three time points by SPSS 13.0 version software.Results　There was no significant differences in Q values between post-1 week and 1 month after LASIK(P>0.05), but significant differences showed when Q values in pre-LASIK compared with post-1 week and 1 month after LASIK, respectively(P<0.01).Conclusion　LASIK could drastically change myopic anterior corneal surface by improving Q values.]]> 2018/11/7 8:22:37 YANG Jia, WANG Ying, LIU Wei-min 962true ［Abstract］　Objective　To study the effects of mangiferin on cell apoptosis and intracellular Ca²⁺ （IECa²⁺）concentration in nasopharyngeal carcinoma CNE3 cells in vitro.Methods　After CNE3 cells were treated with different concentrations of mangiferin for 24,48 and 72 hours, the apoptosis ratio and changes of IECa²⁺ were assessed by flow cytometry.Results　(1)After 24,48 and 72 hours of mangiferin administration, the different concentrations of mangiferin can induced the CNE3 cells apoptosis and the effect were dependent on time and concentration; (2)After CNE3 cells were treated with mangiferin for 24,48 and 72 hours, IECa²⁺ were significantly increased, and the effect were dependent on time and concentration.Conclusion　Mangiferin can markedly induce apoptosis in CNE3 cell, and the increase of IECa²⁺ may play an important role to the induce apoptosis of mangiferin.]]> 2018/9/10 8:34:49 LIU Xiao-chun,LAN Jiao, NONG Chao-zan, et al. 961true ［Abstract］　Objective　To analyze the clinical failure of nickel-titanium instruments and its causes.Methods　Two hundred and seventy-five Hero642 nickel-titanium rotary instruments failed during the clinical root canal instrumentations from May 2007 to April 2010 were collected.The analysis was carried out regarding the relation between the broken files and the distribution in the teeth and canal, and the relation between these files′ size and taper.Results　The majority of the clinical fracture of nickel-titanium instruments was the 0.02 taper and frequently occurred in molar and the midpoint of the curvature.Conclusion　The fracture of nickel-titanium instruments is closely related to the anatomy of the root cana1 and the usage of instrument and so on.]]> 2018/9/10 8:34:49 LI Yan, YANG Jian-shao, LI Shi-jian 960true ［Abstract］　Objective　To study the association between metabolic enzymes polymorphisms of cytochrome P4501A1 and glutathione S-transferase M1, T1 genotypes and childhood acute lymphocytic leukemia. Methods　Using case-control methodology, 90 healthy controls and 89 patients with childhood ALL were studied. The frequencies of the genotypes were detected by PCR-RFLP techniques. Results　Homozygous mutation(C) of CYP1A1 gene (MspⅠ polymorphisms) between patients and healthy controls were statistically significant (P<0.05), the risk of ALL in the individuals with homozygous mutation was higher than that in individuals with heterozygote(B) and wild type(A) (OR=1.997, 95%CI: 1.024～3.896). The null genotype of GSTM1 in childhood ALL group was significantly different from that in the controls (P<0.05, OR=2.709, 95%CI: 1.427～5.146). The null genotype of GSTT1 in childhood ALL group was not significantly different from that in the controls (P>0.05). In the patients with homozygous mutation of CYP1A1 gene combined with the null genotype of GSTM1、 GSTT1, the risk of childhood ALL was increased (OR=2.235, 95%CI: 1.111～4.497). Conclusion　These results indicated that homozygous mutation of CYPlAl gene and the null genotype of GSTM1 alone might be associated with the susceptibility of childhood ALL. The null genotype of GSTT1 alone might not be associated with the susceptibility of childhood ALL. Homozygous mutation of CYP1A1 gene combined with the null genotype of GSTM1、 GSTT1 might serve as a risk factor in the etiology of childhood ALL.]]> 2018/9/10 8:34:49 JIANG Lie-jun,QIN Gui-fang 959true ［Abstract］　Objective　To explore factors which influence survival time in hemodialysis patients suffering from diabetic nephropathy.Methods　Death cases over the period from 1999 to 2008 in Guangxi Hemopurification Centre were retrospectively analyzed, which were divided into group A (<3 months ), B (between 3 months and 3 years ) and C (>3 years) respectively according to the treatment duration.Results　Hydropneumonia and uremia symptoms normally were dialysis indicators among a total of 73 death patients. Cardiovascular disease and cerebrovascular disease turned out to be primary cause of death. Parameters including serum albumin, hemoglobin, parathyroid hormone and creatinine clearance showed significant difference in 3 groups （P<0.05）.Conclusion　Advanced age, low serum albumin, anemia are factors affecting survival time in hemodialysis patients with diabetic nephropathy. Effective pre-dialysis supervision and amelioration of nutritional condition and anemia will benefit the elongation of life span and improvement of quality of life.]]> 2018/9/10 8:34:49 ZENG Chun, ZENG Xi, ZHANG Wen-xin, et al. 958true ［Abstract］　Objective　To evaluate the value of fast-track surgery in radical resection for coloretal cancer.Methods　One hundred and thirty-six patients with colorectal cancer were involved in the non-randomized, prospective controlled study,and were divided into three groups,group 1 containing 50 consecutive patients underwent fast-track colorectal surgery,group 2 ( 21 patients) laparoscopic surgery,and group 3 (65 patients) conventional care. Then their postoperative rehabilitation were observed and compared.Results　Median hospital stay of three groups were 7.0 d,8.5 d, 12.5 d respectively(P<0.05). There was no significant difference in perioperative complications amog three groups(10.0%,14. 3%, 15.2%, P>0.05).Conclusion　Fast-track surgery is able to accelerate the rehabilitation of the patients with radical resection for coloretal cancer,compared to laparoscopic surgery and conventional care.]]> 2018/9/10 8:34:49 YU Jun-ying, FENG Ze-rong, HUANG Shun-rong, et al. 957true ［Abstract］　Objective　To study the effectiveness, safety and tolerance of testosterone undecanoate (TU) in improving quality of sex life of the male continuous hemodialysis patients.Methods　Seventy male continuous hemodialysis patients whose serum total testosterone(T) level was lower than 260ng/dl were randomized into two groups. The treatment group was treated with routine hemodialysis and TU (80 mg/morning and 40 mg/night, after two weeks it was changed to 40 mg/morning and 40 mg/night) and the control group with routine hemodialysis alone. The study was continued for six months. Serum sex hormone of the patients was examined and the international index of erectile function(IIEF) was assessed.Results　The T of the treatment group(273.72±86.52)ng/dl was significantly higher than those of the control group(212.36±69.10)ng/dl( P<0.05), and the IIEF scores was also significantly higher than those of the control group. FSH、LH、E2、PRL、P level were no significant differences between both groups. There were no significant differences in adverse effects between both groups.Conclusion　TU can improve the quality of sex life of the male continuous hemodialysis patients. There is a satisfactory tolerances and few adverse reactions.]]> 2018/9/10 8:34:49 PAN Song-qiu,LI Qi,WU Shu-yue,et al. 956true ［Abstract］　Objective　To evaluate the therapeutic effect and adverse effects of radio frequency ablation(RFA) and transcatheter arterial chemoembolization (TACE) for the treament of live cancer. Methods　In this study,30 patients underwent RFA and 35 patients underwent TACE. After treatment, the main symptoms, AFP level and live function were observed. Meanwhile, ultrasonography and CT scanning were performed. Results　RFA: The complete ablation(CA) rate was 86.96%(20/23) in tumors less than 5 cm in diameter, and 20.59%(7/34) when tumors were larger than 5 cm, the partially or uncompletely ablation in tumors larger than 10 cm, signs of toxicity included pain during operation, nausea fever after operation, transaminase elevation and so on. TACE: lipiodol deposition <50% in part cases; signs of toxicity included fever， myelosuppression,gastrointestinal tract reaction. Conclusion　Treating liver cancer by RFA and TACE is effective and safe, but great care should be taken.]]> 2018/9/10 8:34:49 HU Zheng-cao,PENG Yu-huan,LI Yong-jie,et al. 955true ［Abstract］　Objective　To explore the relationship between the hepatitis B virus and DNA damage, and explore DNA damage situation around the different mode of infection of HBV-infected patients.Methods　Single-cell gel electrophoresis (SCGE) technique was adopted in measuring DNA damage in peripheral blood lymphocytes of controls, different models of chronic HBV-infected patients and the comet analysis software was used for analyzing the results. The degree of DNA damage was judged by the comet tail length (tail extent), the percentage of the comet tail DNA (Tail% DNA), the comet tail of inertia (tail inertia), olive tail moment.Results　There were significantly differences between infection of HBV and DNA damage(P<0.05) ,and also significantly differences between the different mode of infection of HBV-infected patients(P<0.05) after the SCGE .There were a few comet tail images in lymphocytes of xiaosanyang group and obviously comet tail images in lymphocytes of dasanyang group . The tail extent , tail %DNA in lymphocytes of chronic hepatitis B patients were aggravated significantly than in lymphocytes of controls (P<0.05),and there were significantly differences between the tail extent , tail %DNA, tail inertia of the different mode of infection in HBV-infected patients（P<0.05）.Conclusion　Hepatitis B virus is likely to be the main cause of resulting DNA damage in peripheral blood lymphocytes. And different mode of infection of HBV-infected patients have the different damage situation.]]> 2018/9/10 8:35:16 HUANG Jin-mei,ZENG Xiao-yun,QIU Xiao-qiang 954true ［Abstract］　Objective　To observe the clinical curative effect of the “shujing guasha” therapy in prevention and treatment of recurrent respiratory tract infections (RRTI) in children. Methods　The clinical trials were designed by multicentered,double blind, randomized and parallel controlled method. One hundred children with RRTI were divided into two groups at random: the “shujing guasha” therapy group (50 children) and the control group (50 children). The treatment group was treated by “shujing guasha” treatment, the control group was treated by other “guasha” therapy. The treatment course was one month. The follow-up time was 6 months. The clinical curative effect of two groups were observed. Results　The total effective rate of the treatment and the control group were 86.0% and 36.0% respectively, the difference was significant (P=0.000). the “shujing guasha” therapy can significantly reduce the number of upper respiratory tract infection and shorten the course, the difference was significant (P=0.000); and can obviously improve the children′s immune index and general situation, the difference was significant (P=0.000). Conclusion　The “shujing guasha” therapy has good efficacy in prevention and treatment of RRTI children.]]> 2018/9/10 8:35:16 PANG Jun,LIU Zhen-wei,TANG Hong-liang,et al. 953true ［Abstract］　Objective　To investigate the different efficacy in the treatment of ametropic amblyopia by conventional therapy and perceptual learning.Methods　Clinical cases control study. From June 2006 to August 2009, 172 cases of amblyopes, aged from 4 to 7 years old, underwent one year′s treatment in Vision Science and Optometry Center of Guangxi, were recruited in our study. And they were divided into two groups according to the treatment they received. The efficacy of 179 eyes of 92 amblyopes in conventional therapy group and 156 eyes of 80 amblyopes in perceptual learning group were analyzed by Chi-square test with SPSS13.0 software.Results　No significant differences was showed in efficacy between the two groups in the treatment of mild amblyopes(χ²=0.007, P>0.05); but the efficacy of perceptual learning is better than conventional therapy in the treatment of moderate amblyopes(χ²=8.529, P<0.05) and severe amblyopes (χ²=6.430, P<0.05).Conclusion　Perceptual learning is better than conventional therapy in the treatment of amblyopia, reflecting a character of individual treatment for amblyopia.]]> 2018/9/10 8:35:16 LI Zhi-chao, LIU Hong-ting, ZHAO Wu-xiao,et al. 952true ［Abstract］　Objective　To investigate the treatment points of patients with Angle Class Ⅱ division 1 malocclusion treated with Tip-Edge plus technique. Methods　Eighteen patients with angle Class Ⅱ division 1 malocclusion with maxillary protrusion (12 girls and 6 boys, mean age 15.8 years) were selected. All the patients were extracted four first or second premolars and treated with Tip-Edge plus technique. Cephalometric analysis was performed to evaluate the changes before and after treatment. Results　After treatment, all the patients achieved the goal. ANS angle was reduced and U1-NA was decreased significantly (P<0.01). Conclusion　With Tip-Edge plus technique, the patients with Class Ⅱdivision 1 malocclusion with maxillary protrusion can be treated effectively.]]> 2018/9/10 8:35:16 CHEN Shi-wen，ZHOU Yan，FANG Zhi-xin,et al. 951true ［Abstract］　Objective　To summarize and analyze the prevention and treatment of the complication of video-assisted thoracoscopic cardial surgery for 154 patients.Methods　Under peripheral extracorporeal circulation (ECC), the video-assisted thoracoscopic cardial surgery were completed in 154 patients, Among them repair of atrial septal defects were performed in 64 patients, repair of ventricular septal defect in 77 patients, and mitral valve replacement in 13 patients.Results　Repair time of atrial septal defects and ventricular septal defect ranged from 28 minutes to 132 minutes; while the time of mitral valve replacement ranged form 96 minutes to 157 minutes. The operation time ranged form 2.6 hours to 5.6 hours, with a average of 3.6 hours. After operation, 20 patients had complication (12.98%), incision were enlarged during operation in 6 patients (3.90%). Among them, transverse breastbones break occurred in 3 patients; right ventricle rupture during operation in one patient; twice hemostasis after operation in 4 patients (2.60%); liver ruptured in one patient; compartment syndrome of the right lower leg in 2 patients; lung infection in 2 patients; residual shunt of ventricle in 2 patients; operating incision delayed healing in 2 patients. 1 patient died in the whole group (0.69%).Conclusion　Simple cardial operation can be performed under video-thoracoscope. It has advantages of minimum invasion, and good cosmetic effect. It is a feasible method, but it needs plenty of practicing operations with endoscope, otherwise, unskillful operation will increase the occurrence of complication.]]> 2018/9/10 8:35:16 DENG Hai-qing, DU Zheng-long, LING Yi 950true ［Abstract］　In the past eight years of the implementation of nine projects(AIDS) of Municipal Health Bureau in Yizhou Guangxi,four mechanisms including organizational security, system security, human security, and funding security were established. And three measures including grasping training,enhancing skill levels of anti-aids and giving wide publicity, and creating an atmosphere of anti-aids and establishing HIV screening laboratories were made. Four mechanisms and three measures are briefly reviewed. The approach, experience of project implementation and the results achieved provide a useful reference for the current and future work of anti-aids.]]> 2018/9/10 8:35:16 LU Kang-zhi, XIONG Cui-huan, LIAO Dong-ming,et al. 949true ［Abstract］　Objective　To investigate the status of the co-infection of hepatitis B virus (HBV) and hepatitis C virus (HCV) among AIDS in Guangxi Guizhong areas.Methods　From January 2007 to December 2009, the clinical data of 2342 patients with diagnosed AIDS were retrospectively analyzed.Results　There were 16.8% HIV/HBV co-infection, 22.5% HIV/HCV co-infection and 2.5% HIV/HBV/HCV co-infection in all three viruses. In HIV/HCV co-infection patients, 74.7% due to injection drug and 8.0% due to sexual transmission, 15.6% HIV/HBV co-infected by drug injection and 17.2% due to sexual transmission. In HIV/HBV/HCV co-infection patients, 7.8% due to injection drug and 1.0% due to sexual transmission.Conclusion　There was no significant difference between injection drug and sexual transmission in HIV/HBV co-infection. There were significant difference between injection drug and sexual transmission in HIV/HCV co-infection and HIV/HBV/HCV co-infection, we must take measures to control its spread.]]> 2018/9/17 8:12:27 MENG Zhi-hao, WEI Yi，YANG Ri-yao，et al. 948true ［Abstract］　Objective　To evaluate the clinical value of urine liquid based cytology and NMP22 in the diagnosis of bladder cancer.Methods　Urine samples from 208 patients suspected of suffering from bladder caner were detected by liquid based cytology, urine from 42 patients suspected of suffering from bladder caner were detected by NMP22 test, both of results were compared to subsequent histopathology to analyze the sensitivity,specificity,negative predictive value, and positive predictive value of diagnosis for bladder cancer.Results　The sensitivity of liquid based cytology and NMP22 was 71.6%,80% respectively; the specificity was 99.3%,72.7% respectively; the positive predictive value was 97.7%,72.3% respectively;the negative predictive value was 89.5%,80% respectively.Conclusion　Liquid based cytology was a non-traumatic way of diagnosis, the specificity and the positive predictive value are very high. NMP22 test can increase the sensitivity of liquid based cytology.]]> 2018/9/17 8:12:27 YE Jian-bo，ZHU You-kai，ZHOU Xiang-zhen, et al. 947true ［Abstract］　Objective　To search an appropriate method of remedying the patients with Class Ⅲ malocclusion and the best correction time.Methods　One hundred 3～6 years old patients with Class Ⅲ malocclusion were randomly divided into 2 groups, One was treated by reformed move appliance, another was treated by tradition move appliance. After correction the changes of soft and hard tissues were evaluated and analyzed.Results　There were more significant efficiency in the clinical correction and measurement of the changes in the position of mandibular anterior teeth, the concave profile,and soft tissues improving in the group treated by reformed move appliance than those in the group treated by tradition move appliance.Conclusion　The reformed move appliance has both the mechanical and myofunctional effects and befits to patients with Class Ⅲ malocclusion who have scattered space in mandibular anterior teeth and can effectively improve the concave profile.]]> 2018/9/17 8:12:27 CHEN Yu-mei, WANG Fu-jing, XIONG Chun-lan 946true ［Abstract］　Objective　To investigate the changes of tattoos after being tattooed and treated with Nd:YAG laser and to provide some experimental grounds for electing the optimal treating time after being tattooed and laser treating interval.Methods　Forty white mice of Kun Ming kind were tattooed by an amateur artist with black pigment.Some mice were killed at the time to investigate the tattoo particles′ changes under light microscope. After 3 months, Nd:YAG 1064nm laser was used to remove tattoo pigment. Some mice were killed to investigate the tattoo particles′ changes under light microscope at definite time.Results　After being tattooed, the tattoo particles were scattered in superficial and mid dermis with smudged and vagueness border on the first day. In a week, tattoo particles with smooth border located in mid-dermis and surrounded hair follicles. In three months, most of the tattoo particles were located in subcutaneous tissue in clump. In six months, the tattoo particles were located in subcutaneous tissue in a tighten band shape. On the first day after being treated with Nd:YAG laser, the tattoo particles were located in subcutaneous tissue and crushed into consistent sized particles with vague borders. In a week, the borders of tattoo particles turned smooth. In three months, most of tattoo particles disappeared and located in subcutaneous tissue in a band shape with smoother borders.Conclusion　Nd:YAG laser can be used to remove tattoo particles effectively. The optimum treating time was six months after tattooed. The optimum treating interval time was three months.]]> 2018/9/17 8:12:27 XIE Zhi, CHENG Bo, ZHANG Ning 945true ［Abstract］　Objective　To study the risk factors of premature thelarche of girls.Methods　Questionnaire about risk factors of premature thelarche was designed and used.Totally 187 cases of premature thelarche in the People′s Hospital of Guangxi Zhuang Autonomous Region from February 2004 and August 2009 were analyzed by the method of 1∶1 paired control study. According to the single factor analysis, the significant factors were analyzed with the multi-factors conditional Logistic regression analysis.Results　In the single factor analysis, 7 of 16 indexes were significant; while in the multi-factors stepwise regression analysis, 5 of 16 indexes were significant.Conclusion　It is shown that the concentration of estrogen, the concentration of follicle stimulating hormone, the first menarche of mother,the breast of tanner,the first growth of breast may be the risk factors of premature thelarche of girls.]]> 2018/9/17 8:12:27 LIANG Zhen-hua，DAI Yi,LU Mei-hua 944true ［Abstract］　Objective　To detect the mRNA expression of the CTA (NY-ESO-1) in retinoblastoma (RB) tissues and investigate the possibility of applying this antigen as the target antigen for RB specific immunotherapy.Methods　With reverse-transcription polymerase chain reaction (RT-PCR), the mRNA expression of NY-ESO-1 in 15 cases of RB tissues, 12 cases of nontumorous retina tissues and 22 cases of normal ocular region tissues were detected. The results of expression and related clinical parameters were analyzed with statistic analysis software.Results　Among 15 cases of RB tissues, NY-ESO-1 mRNA was detected in 40%（6/15）, NY-ESO-1 can′t be detected in the 12 cases of nontumorous retina tissues and 22 cases of normal ocular region tissues.Conclusion　With expressive frequency in RB, no relationship was found between the expression of NY-ESO-1 genes and related clinical parameters(P>0.05). NY-ESO-1 might be potential target antigen for specific immunotherapy of RB.]]> 2018/9/17 8:13:20 LIN Quan，LIANG Hao，YAN Yu qing，et al. 943true ［Abstract］　Objective　To investigate the NF-кB expression on adrenocortical carcinoma and its clinical significance.Methods　NF-кB expression in clinical tissues(15 case of adrenocortical carcinoma, 30 case of adrenocortical adenoma, 11 case of adrenal hyperplasia and 7 case of normal adrenal cortex) were detected by immunohistochemistry.Results　The expression of NF-кB in adrenocortical carcinoma tissue was positive in 9 of 15 cases, including strong positive expression in 2 cases, positive expression in 4 cases，weak expression in 3 cases，negative expression in 6 cases;The expression of NF-кB in adrenocortical adenoma tissue was positive in 8 of 30 cases, including strong positive expression in 0 case, positive expression in 2 cases, weak expression in 6 cases, negative expression in 22 cases; in adrenal hyperplasia tissue, there was weak positive expression in 2 cases, the remaining were all negative expression; there was no positive expression in normal adrenal cortex tissue. The positive expression in adrenocortical carcinoma was higher than that in other adrenocortical tissue, the expression had significantly statistical difference between adrenocortical carcinoma and adrenocortical adenoma.Conclusion　NF-кB is significant in diagnosing adrenocortical carcinoma.]]> 2018/9/17 8:13:20 JIANG Li,LUO Zuo-jie,KUANG Xiao-cong,et al. 942true ［Abstract］　Objective　To evaluate the value of the diameter of fetal umbilical artery and umbilical vein in the prenatal diagnosis of thalassemia.Methods　One hundred and twenty fetus of thalassemia verified by molecular biologic technique were detected by two-dimensional echocardiography and Doppler ultrasonography；106 normal fetus were used as control group. The diameters of fetal umbilical artery and umbilical vein were detected by ultrasound, the diameter ratio(umbilical artery / umbilical vein) was measured. Results　The diameters of fetal umbilical artery and umbilical vein of thalassemia group were obviously larger than those of control group， there was statistical significance (P<0.05). The diameter ratio of thalassemia group was bviously lower than that of control group， there was statistical significance (P<0.05). Conclusion　As a non-invasive technique, ultrasound detection of the diameter of fetal umbilical artery and umbilical vein has a certain value in the prenatal diagnosis of thalassemia.]]> 2018/9/17 8:13:20 WAN Li-kai，CHEN Yan-hong，XU Chun-mei，et al. 941true ［Abstract］　Objective　To investigate the accuracy of Wavescan wavefront aberrometer in measuring the lower order aberrations.Methods　Ninety-one myopia patients were selected randomly from the LASIK patients in our hospital from April to October,2008. The patients were divided into two groups according to the age: refractive status were measured by wavefront aberrometer and cycloplegic retinoscopy refraction in group A, while the wavefront aberrometer and noncycloplegic retinoscopy refraction were employed in group B, the sphere, cylinder and cylindrical axis were analyzed.Results　The sphere, cylinder and cylindrical axis measured by the aberrometer and the cycloplegic pupil had significant difference (P<0.05) in group A. The sphere, cylinder and cylindrical axis measured by the aberrometer and the noncycloplegic pupil also have significant difference (P<0.05) in group B. However, the refractive error measured by noncycloplegic retinoscopy refraction was more closed to the sphero-cylinder result of the wavefront analyzer than those measured by cycloplegic retinoscopy optometry. The consistence of low-order aberration between the wavefront analyzer and non-cycloplegic retinoscopy optometry was better than those between the wavefront analyzer and cycloplegic retinoscopy optometry. The pupil diameter of the right eye was larger than the left eye′s in the male; the similar result were observed in the female.Conclusion　The difference of the refractive value between the wavescan abrrometer and objective retinoscopy refraction was observed, which was likely attributed to the accommodation or the different precision of the optometry method, that means we need to take compounding factors such as accommodation, pupil diameter and compliance of patients into account when the refractive error be measured by wavefront analyzer.]]> 2018/9/17 8:13:20 WANG Ying, LIU Wei-min, XIAO Xin, et al. 940true ［Abstract］　Objective　To evaluate the general effects of appropriate health technique (AHT) of TCM in the process of application in community health service (CHS). Methods　Based on the questionnaire designed by ourselves, we train those who were supposed to be investigated. Afterward, 441 of those who were ever treated by appropriate health technique of TCM to community health service took a real time investigation in light of the guidance of staff. The results were then used to make a general evaluation of appropriate health technique in terms of application achievements, effective rate, hygienic economics and patients satisfaction. Results　The total degree of satisfaction of patients who were treated by appropriate health technique of TCM to community health service was 86.15%. The satisfaction of different appropriate health techniques was around 80%～95.8%. Patients′ education situation and the mode of payment had significant effect on the degree of the satisfaction （P<0.01）. Conclusion　The application of appropriate health technique of TCM in community health service got a fine effect,a low payment and a nice judgment.]]> 2018/9/17 8:13:20 LIANG Jian，DENG Xin，YANG Rui-chun,et al. 939true ［Abstract］　Objective　To explore the short-term efficacy of intramedullary controlled dynamic nailing in the treatment of midshaft femoral，tibial and humeral fractures.Methods　The clinical records of patients with midshaft fractures treated with intramedullary controlled dynamic nailing from August 2008 to August 2009 were retrospectively analyzed. During operation, closed reduction was applied under C arm X ray machine. Finite open reduction and internal fixation was used in patients who failed in closed reduction. Autologous bone grafts or combined bone grafts was used in grave comminuted fracture.Results　All the patients were followed up from 3 months to 14 months, with an average of 8.5 months.Of 19 cases,18 cases were cured, the cure rate was 94.7%. the mean healing time was (16.5±4.2) weeks. Knee stiffness occurred in one patient, no breakage and exiting of nails and locked nails occurred.Conclusion　It can get satisfactory short-term efficacy and high cure rate in treatment of midshaft femoral ，tibial and humeral fractures by intramedullary controlled dynamic nailing.It is one of the good techniques in treating midshaft fractures.]]> 2018/9/17 8:13:20 LI Xin-xu,LUO Xiao-dong,WANG Zhi-zhong,et al. 938true ［Abstract］　Objective　To investgate the clinical value of fluorescence in situ hybridization (FISH) in number abnomality of fetus chromosomes for rapid prenatal diagnosis.Methods　The interphase nuclei of amniotic fluid from 123 pregnant women according with the indication of prenatal diagnosis were detected by chromose - specific DNA probes of 13, 18, 21, X and Y chromosomes, the routine analysis of amniotic cells karyotypes was also performed at the same time.Results　In the 123 samples, results of chromosomes 13, 18, 21, X and Y were concordant with diagnostic outcome of conventional chromosome karyotype analysis. One hundred and twenty detections showed normal chromosome. In 3 abnormal chromosome samples, trisome 21 were detected in 2 samples and trisome 18 was detected in 1 sample.Conclusion　FISH technique exhibited the characters of rapidness, simplicity, high specificity and sensitivity, so it is a reliable and a rapid technique for prenatal diagnosis.]]> 2018/9/28 10:50:37 MO Yao-xi, HUANG Li, HE Bing, et al. 937true ［Abstract］　Objective　To study the lipid metabolism of polycystic ovary syndrome (PCOS) patients with the different obesity standards, and to provide the basis for prevention and treatment of cardiovascular disease in these women. Methods　With body mass index (BMI) ≥24 kg/m² for the obesity standards, PCOS patients were divided into obese A group and non-obese A group; With waist-hip ratio (WHR) ≥ 0.8 for the obesity standards, PCOS patients were divided into obese B group and non-obese B group; With BMI ≥ 24 kg/m² and WHR ≥ 0.8 for the obesity standards, PCOS patients were divided into obese C groups and non-obese C group. The levels of triglycerides(TG）, total cholesterol(TC）, high density lipoprotein cholesterol(HDL-C）, low density lipoprotein cholesterol(LDL-C）, apolipoprotein(a)-1(ApoA1）, apolipoprotein(b)(ApoB） and lipoprotein a(Lp-a）were measured in these patients. Results　（1）The TG level in obese A group was higher than that in non- obese A group (P<0.05), the TC and LDL-C level in obese B group was higher than that in non-obese B group (P<0.05); The TG level in obese C group was higher than that in non- obese C group (P<0.05); （2）Multivariate analysis showed that WHR and BMI were associated with HDL-C（P<0.05）. Conclusion　In PCOS patients abnormal lipid metabolism and obesity are closely linked. Obese PCOS patients should be morniterd and treated to prevent and reduce the occurrence and development of cardiovascular disease.]]> 2018/9/28 10:50:37 HUANG Ka-li,JIANG Feng-yan,LI Hong-fang 936true ［Abstract］　Objective　To study the safety and feasibility of laparoscopic surgery and Parks surgery modified for low rectal cancer.Methods　The patients′ clinical data of laparoscopic surgery and Parks surgery modified for low rectal cancer was analysed retrospectively in our hospital between May 2007 and February 2010.Results　Sixteen cases were selected in this study and all surgery was performed successfully. The average operative time was（161.2±55.4）min, the mean volume of bleeding was（146.7±94.5）ml, postoperative time of bowel gas passage was（3.2±0.8）d, the volume of abdominal drainage was（190.2±67.3）ml. One cases experienced anastomotic leakage and got recovery by conservative treatment. No other complications were experienced, such as ureteral injury, dysuresia, fecal incontinence, and so on. The time of following up ranged from one to thirty-one months and the median number was fourteen. Two cases were lost during following up. Seven cases suffered increased frequency of stool and all these patients got normal stool within one year. One case had to accept second surgery for recurrence. It was found by endoscopic examination at postoperative ninth month. No recurrence and matestasis were found in other cases.Conclusion　This short-term study reveals that it is safe and efficient to perform laparoscopic operating and Parks surgery modified for low rectal carcinoma.]]> 2018/9/28 10:50:37 WU Dong-bo, HUANG Shun-rong, WU Hong-gen, et al. 935true ［Abstract］　Objective　To investigate the relationship between the blood stasis syndrome and parts of the risk factors of lumbar intervertebral disk protrusion (LIDP)and transforming growth factor β1 (TGF-β1)-509C/T site gene polymorphisms.Methods　A total of 120 Han ethnic patients from Guangxi Zhuang Autonomous Region were selected according to standard of LIDP, 60 with blood stasis syndrome and the other 60 without, then, their single nucleotide polymorphism (SNP) of TGF-β1-509C/T and lumbar burden intensity were tested, and their relationship with the blood stasis syndrome of LIDP were analyzed.Results　It was showed by Logistic Regression Forward (LR) analysis that risk of syndrome blood stasis of LIDP in patients with severe lumbar burden was 3.977 times （95%CI:1.012～3.801）of that in patients with mild and middle lumbar burden (P<0.05), and the risk further increased 5.365 times（95%CI:1.086～ 2.703） after it′s accumulating with TGF-β1-509C/T site gene polymorphisms in Han ethinic group in Guangxi(P<0.05).Conclusion　Severe lumbar burden may be one of the risk factors of blood stasis syndrome of LIDP in Han ethnic population from Guangxi. Patients with TGF-β1 gene-509 adiponectin CT genotype and combined severe lumbar burden are susceptible to blood stasis syndrome of LIDP.]]> 2018/9/28 10:50:37 XU Jian-wen, ZHONG Yuan-ming, YANG Guang, et al. 934true ［Abstract］　Objective　To observe the efficacy of different surgical treatments for neovascular glaucoma (NVG). Methods　Forty-four cases (44 eyes) with neovascular glaucoma were randomly divided into two groups. In the observation group, first semiconductor laser was used for the closure of neovascularization of the iris surface and anterior chamber angle, on the following day a combined trabeculectomy was performed. In the control group, transscleral cyclophotocoagulation was performed. In two groups postoperative visual acuity, intraocular pressure(IOP), iris neovascularization, and complications were recorded. One year after operation the clinical effects of the two groups were compared. Results　One year after operation IOP both groups in were decreased compared with the preoperative IOP; IOP in the observation group was significantly lower than that in the control group, the difference was statistically significant (P<0.05). Complications in the observation group was lower than that in control group. Conclusion　Semiconductor laser iris photocoagulation combined with compound trabeculectomy and transscleral cyclophotocoagulation may be used as the effective methods for the treatment of NVG, and based on the different conditions of the NVG patients, the different surgical treatments may be used.]]> 2018/9/28 10:50:38 ZHONG Shan,LI Li,LAI Xiao-ling,et al. 933true ［Abstract］　Objective　To summarize the characteristics of symptoms and the experience of treatment of tetramine poisoning.Methods　The clinic datas of 8 cases of acute tetramine poisoning were analyzed.Results　All of 8 cases were cured successfully, and secondary- poisonning was found in one of them.Conclusion　The incubation period of tetramine poisoning by mouth is short, and the main clinical manifestations include convulsion and systemic hyperspasmia. The key points of sucessful cure include thorough gastric lavage in the earlier period, prevention and treatment of brain edema and control of the epilepsy seizure. The treatment of phenobarbital should be used earlier, and the decrease of drug dose should be performed slowly, and the better curative effect should be obtained with the combination of hemoperfusion and comprehansive therapy.]]> 2018/9/28 10:50:38 QIN Wei-ping,GE Xian-min, LIU Xiao- min 932true ［Abstract］　Objective　To observe the effects of eletro-acupuncture stimulation of Zusanli and Neiguan acupoints on neurological motor function and cerebral histopathology of rats with cerebral infraction,thereby to intensively investigate the mechanism of electro-acupuncture on cerebral infarction rats.Methods　Sixty SD healthy adult male rats were divided randomly into three groups: sham operation group, acupuncture group and non-acupuncture group. Each group was randomly divided into 3 subgroups (1-day subgroup, 7-day subgroup and 14-day subgroup). Model of cerebral infarction on rats of acupuncture group and non-acupuncture group was made by the thread embolism method.The rats of acupuncture group were given electro-acupuncture treatment 20 minutes each day on the acupoints of Zusanli (ST36) and Neiguan (PC6), while the rats of rest group were just fixed. The neurological function and balance test were examined at 1^st day, 7^st day and 14^st day on all rats and detected the changes of the histopathology of injury area by hematoxylin-eosin staining.Results　The data showed no obvious difference among 3 groups after 1d (P>0.05);Comparing with non-acupuncture group, both the scores of neurological function and balance test of rats in acupuncture group were lower (P<0.05) at 7^st day and 14^st day; Results from light microscopy observation showed that the damage neurons of penumbrazone in group the acupuncture were lighter than non-acupuncture group1 at day after acupuncture and there was significantly better than non-acupuncture group at 7^st day and 14^st day.Conclusion　It was suggested that eletro-acupuncture stimulation of Zusanli and Neiguan acupoints could improve the neurological deficits and cerebral histopathology after cerebral infarction.]]> 2018/9/28 10:50:38 ZHOU Yuan-cheng, WU Xin-gui, XIAO Yi-cai, et al. 931true ［Abstract］　Objective　To study the effects of hypertonic saline/colloid on fluid balance and clinical outcome during perioperative period.Methods　forty-four patients with gastrointestinal carcinoma undergoing radical surgery were studied. The pairing patients were assigned to receive either hypertonic saline/colloid (the experimental group, n=22) or conventional solution(the control group, n=22)during the early postoperative period. Fluid infusion volumes, urine outputs, as well as total plasma protein, total albumin were compared between the two groups.Results　Urine outputs during the first postoperative 24 hours in experimental group were significantly more than that in control group, but urine outputs in the third postoperative 24 hours in experimental group were significantly less than that in control group（P<0.05）. Fluid infusion volumes during the operation, the first postoperative 24 hours, the second postoperative 24 hours and second postoperative 24 hours in experimental group were no significantly different from that in control group（P>0.05）. Urine outputs during the intrapostoperative period and the third postoperative 24 hours were no significantly different from that in control group（P>0.05）. Total protein and total albumin on the preoperative day and the first postoperative day in experimental group were no significantly different from that in control group（P>0.05）.Conclusion　Hypertonic saline/colloid has an intense diuretic effect, which could accelerate appearance of the top of urine outputs more early after the surgery, as well as reduce the effusion of third space after radical surgery for gastrointestinal carcinoma.]]> 2018/9/28 10:51:43 MAI Wei, MAN Xiao-bo,HUANG Shun-rong,et al. 930true ［Abstract］　Objective　To investigate the effect of intracoronary transplantation of autologous bone marrow mesenchymal stem cell on left ventricular function and the vascular endothelial growth factor (VEGF) in patients with acute myocardial infarction.Methods　After successful percutaneous coronary intervention (PCI) for acute myocardial infarction, 25 patients were randomly assigned to either a control group (n=15) that received optimum postinfarction medical treatment, or a bone-marrow-cell group (n=10) that received optimum medical treatment and intracoronary transfer of autologous bone-marrow cells 14 days after PCI. Data about left ventricular ejection fraction (LVEF) were obtained by echocardiogram before and at 6 months′ follow-up. The serum levels of VEGF were measured by ELISA at 3-day, 7-day, 14-day, 28-day, 56-day time-points after transplatation, respectively.Results　There was no significant difference between the two groups in LVEF before intracoronary transplantation of autologous bone marrow stem cell (P>0.05). After 6 months, LVEF in treated patients was improved significantly (P<0.05) compared with the control, though there was no significant difference in the rate of re-infarction, re-myocardial perfusion and death. The serum levels of VEGF in patients accepted transplation were significantly higher than the levels in control patients (P<0.05).Conclusion　After intracoronary transplantation of autologous bone marrow stem cell in patients with acute myocardial infarction, the left ventricular function could be improved and the heart failure be decreased by the way to increase serum level of VEGF.]]> 2018/9/28 10:51:43 LI Chang-jiang, YIN Zuo-min, WANG Yan, et al. 929true ［Abstract］　Objective　To observe the clinical efficacy of colon therapy machine in the treatment of bezoar ileus. Methods　Three hundreds and five cases according to diagnostic criteria of bezoar ileus were randomly divided into conservative treatment group (75 cases), surgical treatment group (75 cases) and colon therapy mechine group (155 cases). Three groups received fasting, water deprivation, continuous gastrointestinal decompression, correcting water, electrolyte disorders and acid-base balance and other symptomatic treatment. On a basis of above treatment， the surgical treatment group was treated by surgical therapy, the colon therapy machine group was treated by colon therapy machine. Three groups were treated for 6 days （a course of treatment）. After one course of treatment their therapeutic effect were observed. Results　The cure rate of the conservative treatment group was 53.33%, that of the surgical treatment group was 100.00%, that of the colon therapy mechine group was 96.77%. The cure rates in the surgical treatment group, and the colon therapy group were higher than that in the conservative treatment group (P<0.05). Between the colon therapy mechine group and surgical treatment group, there was no significant difference in cure rate (P>0.05). The incidence of complications in the conservative treatment group was 3.23%, that in the surgical treatment group was 29.33%, that in the colon therapy mechine group was 6.45%. The incidences of complications in the surgical treatment group were higher than those in the colon therapy mechine group， and the conservative treatment group (P<0.05)； Between the colon therapy mechine group and the conservative treatment group, There was no significant difference in the incidence of complications (P>0.05). Conclusion　The treatment efficacy of the colon therapy machine and surgical treatment for bezoar ileus is close, but the colon therapy mechine has lower side effects and shorter hospitalization time.]]> 2018/9/28 10:51:43 HUANG Pei-ning 928true ［Abstract］　Objective　To explore the relationship between psychological problems in vocational high school students and parental rearing behaviors.Methods　It was a cross-sectional study.By means of cluster sampling,336 students aged from 16 to18 years old selected from a vocational high school in Beihai were investigated with Symptom Check List-90 (SCL-90),the Egna Minnen Barndoms Uppfostran ( EMBU) scale and the general formation questionnair.Results　The prevalence of psychological problems was 12.4 %（38/306）.Correlation analysis showed that the harsh total score and factor-scores of SCL-90 had positive correlation with parental punishment,excessive interference, overprotection, rejection and preference. The total score and factor-scores of SCL-90 was negatively related to parental emotional concern.Conclusion　Psychological problems in vocational high school students are closely related to parental rearing behaviors,and incorrect parental rearing behavior is one of the risk factors of psychological problems in the vocational high school students.]]> 2018/9/28 10:51:43 JIA Xiao-fei, WANG Ling 927true ［Abstract］　Objective　To study the clinical significance of changes of the level of T lymphocyte subsets and NK cells in peripherial blood of early metaphase patients with NSCLC.Methods　Flow cytometry was employed to detect T lymphocyte subsets and NK cells in peripherial blood from 40 patients with NSCLC(Ⅱ～Ⅲ_b ) (A group) and 15 patients with benign tumor of lung (B group) needed surgery with 40 healthy adults as the controls(C group).The blood specimens were taken on the postoperative 10th days and T lymphocyte subsets and NK cells were defected in A group and B group.Results　Compared with the B group and the C group, the Indicators were significantly higher (P<0.05) in the A group except CD8⁺. The level of CD8⁺ cells were significantly higher (P<0.05) in the group of thoracic exploration than the group of complete resection and partial resection on the postoperative 10th days. The significant differences between the group of complete resection and partial resection did not be found(P>0.05).Conclusion　Monitoring T lymphocyte subsets and NK cells in peripheral blood can be used to detect the patients immunologic function of patients with NSCLC. Especially The CD4⁺/CD8⁺ ratio can reflect the pathogenetic condition of patients with NSCLC. Complete resection and partial resection can improve the immunologic function of patients with NSCLC.]]> 2018/9/28 10:51:43 WANG Huan, LIN Hui, LAN Jiao，et al. 926true ［Abstract］　Objective　To study the change of blood pressure (BP)in patients with obstructive sleep apnea-hypopnea syndrome(OSAHS)，and explore the relationship between OSAHS and BP.Methods　Two hundred and six sleep snoring patients (168 OSAHS and 38 simple snoring),and 30 healthy persons were enrolled. All subjects were tested polysomnography(PSG) and BP.BP was recorded and compared among the simple snoring and OSAHS and healthy persons，and the correlations were analyzed between hypopnea index(HI),apnea index(AI),apnea/hyponea index(AHI), minimal oxygen saturation (SaO₂) during sleep, average SaO₂ during sleep and total sleep time of SaO₂<90%(T90) and the morning diastolic blood pressure (DPB).Results　The blood pressure before sleep and that in the morning between control and simple snoring groups showed no significant difference. The morning DBP was significantly higher than that before sleep in OSAHS group,but systolic blood pressure(SBP) showed no significant difference. The morning DBP was positively correlated with HI,AI,AHI, average SaO₂ during sleep and T90,but negatively with minimal SaO₂ during sleep.Conclusion　OSAHS is an independent risk factor for hypertension.Turbulence of sleep-disordered breathing may be the major pathogeny of hypertension in OSAHS patients.]]> 2018/10/8 8:27:44 WANG Wu,GUO Yong-zhong,LIU Jian-hong,et al. 925true ［Abstract］　Objective　To analyze the relationship between the clinical manifestations and the urine arsenic value in 452 cases of subacute arsenic intoxication. Methods　The clinical manifestations, the diagnosis and the laboratory check result in 452 cases of subacute arsenic intoxication led by the tap wate of arsenic pollution were analyzed. Results　The arsenic content of tap water was 3.19 mg/L and 3.26 mg/L, exceeded 318 times and 325 times of the allowed figures. The urine arsenic value initially examined in 452 cases were 0.24～11.2 mg/L (1.41±1.52 mg/L). In the 452 patients, 389, 249 and 359 cases presented the symptoms of digesting system, the cardiovascular system and nervous system respectively, and 237, 98, 76, 91, 140 and 106 cases presented anomalies of cardiac muscle enzyme, the liver function, the kidney function, the routine blood test, the routine urine test, and electrocardiogram respectively. The numbers of patients diagnosed as the light, moderate,and severe poisoning were 370, 74 and 8 cases respectively. In these patients, the urine arsenic content was positively correlated with the injured organ number and the diagnosis gradation (r=0.552, r=0.678, P<0.01, respectively). Conclusion　On the basis of analysed results, the urine arsenic content is a important indicator for diagnosis of the subacute arsenic intoxication and grades.]]> 2018/10/8 8:27:44 NONG Kang, GE Xian-min, SU Su-hua, et al. 924true ［Abstract］　Objective　To study the synthesis of the N-alcohol erucamide and its activity of anti-tumor in vitro.Methods　(1)Through the micro-organic synthesis technology, erucic acid and methanol were coupled with ethanolamine as raw matierals, sodium as catalyst to synthesize the N-alcohol erucamide, isolation and purification achieved by silical gel column chromatography, and Its structure was confirmed by infrared spectra and nuclear magnetic resonance spectrum; (2)BEL-7404 was cultured in media added to the N-ethanol erucamide.The cells′s growth was observed in inverted microscope. The effect of inhibiting cancer cells was detected with MTT.Results　The N-alcohol erucamide was synthesized successfully；Its strong inhibiting effect was recorded beyond 50.0 μg/ml.The inhibiting rates were all beyond 40%, the inhibitory concentration 50% (IC50) was 101.77588 μg/ml.Conclusion　Through the microscale organic synthesis, N-alcohol erucamide can be synthesized quickly and conveniently. N-alcohol erucamide has obvious inhibiting effcet on BEL-7404 cells in vitro.]]> 2018/10/8 8:27:44 GAO Ming-xing, QIN Qing, MO De-huan,et al. 923true ［Abstract］　Objective　To find out the clinical characteristics of young hypertension patients in towns, and to provide the evidence for the measures to control hypertension in young people in towns in ordar to achieve the standard.Methods　The cardiovascular risk factor of 306 young hypertension patients who received physical examination in our hospital from October, 2007 to May, 2008, were evaluated and their results were compared with those of the 300 elderly hypertension patients who were greacter than or equal to 60 years old at the corresponding period and the clinical characteristics of young hypertension patients were analyzes in order to intervene it aiming at its characteristics.Results　Compared with the aged, the young were higher in average diastole pressure, hypertensive family history,over -weight, smoking, excessive drinking, impaired fasting glucose, hypercholesterolemia,high triglyceride and hyperuricemia.The relevance rate of hypertension in young people in towns was 11.7 percent, the awareness rate of hypertension was 11.44 percent, treatment rates was 7.19 percent, control rate was 3.92 percent, which were lower than those of the aged. Conclusion　Young hypertension patients should be educated in a healthy way of life in order to find and prevent and treat the disease earlier, and increase the awareness rate of hypertension, treatment rate and the control rate of blood pressure.]]> 2018/10/8 8:27:44 XIE Yan-ping, SHEN An-huang, WANG Shao-ping, et al. 922true ［Abstract］　Objective　To investigate the effect of edaravone on plamas levels of C3,IL-6 and IL-8 in patients undergoing on-pump beating heart surgery. Methods　Sixty patients with rheumatic heart disease undergoing mitral valve replacement were divided into two groups: edaravone group (group E, n=30): patients received edaravone injection 0.5 mg/kg in circuit priming fluids; Control group(group C, n=20): patients received equal volume of normal saline. C3, IL-6 and IL-8 were respectively measured at six different time intervals (preoperation, 30 minutes after CPB, termination of CPB, postoperation, 24 hours and 72 hours after postoperation). Results　(1)The levels of plasma C3 in both groups were declined from the beginning of CPB, reached trough at postoperation, elevated from the time of 24 hours after postoperation, restored to normal at the time of 72 hours after postoperation, there is no statistical difference between groups (P>0.05). (2)The levels of plasma cytokines in both groups were elevated from the beginning of CPB, reached peak at postoperation, declined from the time of 24 hours after postoperation, restored to normal at the time of 72 hours after postoperation, the levels of edaravone group were lower than those of control group(P<0.01). Conclusion　Edaravone can decrease the plasma levels of inflammatory cytokines such as IL-6 and IL-8, alleviate the postoperative systemic inflammatory response in cardiac surgery.]]> 2018/10/8 8:28:25 LIU Jun,XIAN Lei,HE Wei,et al. 921true ［Abstract］　Objective　To use the new microwave thawing devices for thawing frozen red blood cells, test quality control indicators, and evaluate the new microwave thawing devices and the effects of red blood cell preparation.Methods　The new microwave thawing devices were used for thawing the red blood cells preserved in glycerol -80 ℃, the glycerol was removed with different concentrations of washing liquid, the red blood cell recovery, free hemoglobin, residual glycerol content, in vitro hemolysis test and the sterility test were used as for the quality inspection control project.Results　The new microwave thawing devices can quickly thaw the frozen red blood cells, the quality indicators of thawed red blood cells were in accord with “whole blood and component quality requirements” (GB18469-2001), the recovery rate of red blood cells was markedly improved and the preparation time was shortened.Conclusion　The new microwave thawing devices can quickly thaw frozen red blood cells, the quality was in accord with standard. It provide new ways and means for the long-term storage of blood,guarantee of the blood supply,blood transfusion in patients with rare blood groups and blood transfusion itself, especially in first aid of the field, natural disasters and other special conditions it can play a role, it is worth popularizing.]]> 2018/10/8 8:28:25 ZHANG San-ming,ZHOU Hong-yu,TU Hong-gang,et al. 920true ［Abstract］　Objective　To study the optic disc parameters of normal people in Han nationality in Guangxi. Methods　One hundred and twenty blood-unrelated nomoral volunteers living in Lonlin region of Guangxi were involved in the study and divided into 4 groups accoding to their ages. Heidelberg Retina Tomograph(HRT) was used to obtain the optic disc parameters. Results　The parameters of ONH in normal eyes are as follows: DA（2.2165±0.4266）mm², CA（0.4816±0.2979）mm², RA（1.7311±0.3217）mm², C/DAR（0.2074±0.1092）, R/DAR（0.7923±0.1091）, CV（0.1076）mm³, RV（0.5196±0.1519）mm³, MCD（0.2055±0.0798）mm, MxCD（0.6039±0.1949）mm, HVC（0.4513±0.1639）mm, CSM（-0.2155±0.0617）mm, mRNFLT（0.3059±0.0845）mm, RNFLA（1.5785±0.3830）mm². The parameter of mRNFLT and RNFLA were significant difference between the group of more than 50 years and other groups of less than 49 years in Han nationality. There was not significant diference between right and left eyes and between different genders in all parameters. The binocular difference value of C/DAR was 0.048～0.112 in Han nationality. The parameters of RV and HVC were unaffected by disc area. Conclusion　HRT can be used to measure optic disc parameters of normal person in Zhuang nationality. There were not differences in between diferent genders and two eyes. RV and HVC appears to be a good parameter for evaluating.]]> 2018/10/8 8:28:25 LI li,YANG Ke, BAI Hai-qin 919true ［Abstract］　Objective　To evaluate the clinical mechanism of the bidimensional（2D） self-ligating lingual brackets technique by comparing the data of the pretreatment and posttreatment cephalometric films and provide the supports to the clinical generalization. Methods　Twelve adults between 21 to 49 years old, 4 with Angle classⅠlight crowding, 5 with Angle class Ⅱ¹ deep over-bite and over-jet, and 3 with Angle class Ⅲ anterior cross bite were accepted and treated with the bidimensional self-ligating lingual brackets technique. 8 were treated with nonextraction and 4 with extraction. The thermodynamic NiTi round wire, steel round wires or Australian wire but not set-up and complicated indirect brackets bongding technique were used. The intermaxillary elastics and power chains were used to adjust the occlusions, close the extraction space and do some simple torque controlling. Seven cephalometric parameters which represented the sagittal and vertical changes of jaw bones and teeth were used to evaluate the changes of jaw bones, teeth and profiles of patients treated by the bidimensional self-ligating lingual brackets technique. Results　The changes of cephalometric parameters except U1 to NA in Angle class Ⅰpatietns were not significantly different(P﹥0.05); U1 to L1 angle changed from 109°to 126° in cases of Angle class Ⅱ (P<0.05); The changes of U1 to NA angle and U1 to L1 angle were significantly different in cases of Angle class Ⅲ (P<0.05). All cases achieved the objective of invisible, and at the same time, gained a satisfactory effect. The treatment time were 11～19 months. Conclusion　The changes of protrusion of anterior tooth were significant in Angle class Ⅱ and Ⅲ cases except Angle class Ⅰ when use the bidimensional self-ligating lingual brackets technique. It has a favourable generalizing perspective in the correction of light skeletal malocclusion of adults, some nonextraction cases in which teeth need not to be moved much, and some extraction cases.]]> 2018/10/8 8:28:25 ZHOU Yan, FANG Zhi-xin,CHENG Shi-wen，et al. 918true ［Abstract］　Objective　To explore the effect of lateral position ventilation in infant with extracorporeal circulation hypoxia.Methods　Lateral position ventilation was used in 18 infant patients with extracorporeal circulation hypoxia. Blood gas analysis was conducted before ventilation and 1 hour after lateral position ventilation and 1 hour after supine ventilation was resumed to observe the effect of different body position for ventilation on respiratory and circulatory parameters of patients.Results　After 1 hour of lateral ventilation, their PO₂ and PaO₂/FiO₂ was higher than those before ventilation（P<0.01）,there was no significant differences in respiratory and circulatory parameters between the two positions（P>0.05）;One hour after resumed supine ventilation, the PO₂ and PaO₂/FiO₂ were became lower but still higher than those before ventilation（P<0.01）. Conclusion　Lateral ventilation is obvious effective for infant with extracorporeal circulation hypoxia.]]> 2018/10/8 8:28:25 WU Yan, WEI Jing, NA Wen-yan,et al. 917true ［Abstract］　Objective　To construct RNAi recombinant adenovirus expressive vectors specific to GSK-3β and explore if it can inhibit the GSK-3β expression of human umbilical vein endothelial cells induced by high free fatty acid.Methods　Homologous recombination and cloning techniques were used to construct RNAi recombinant adenovirus expressive vectors specific to GSK-3β. Then, the adenovirus plasmids was transfected into HEK293A cells to produce adenovirus and amplify the adenovirus stock. Plaque forming assay was used to titer the adenovirus stock. The GSK-3βexpression were detected by Western blot.Results　The RNAi adenovirus vectors specific to GSK-3β were successfully produced with high titer. The expression of GSK-3β protein could be down-regulated efficiently by the RNAi adenovirus in HUVEC both in the condition of normol and high free fatty acid(P<0.05).Conclusion　RNAi adenovirus is an important tool that inhibit the expression of GSK-3β efficiently. It may partly protect HUVEC from impairment which induced by FFAs.]]> 2018/10/8 8:28:53 CHEN Yu-fang, CHEN Gang, LIN Li-xiang 916true ［Abstract］　Objective　To study safety, effect and scope of the ketamine and magnesium(KMS) intravenous combined anesthesia in clinic.Methods　（1）On the basis of the animals and clinical preliminary study, the best compatibility proportion of three different concentrations of KMS(KMS composite liquid Ⅰ, Ⅱ, Ⅲ) were identified. Intravenous drip of diazepam 0.1～0.2 mg·kg^-1 was first used for anesthesia induction, subsequently intravenous drip of KMS composite liquidⅠ was performed.According to anesthesia need anectine 100 mg was used for intubation. A dose of KMS composite liquid Ⅱ was first used, then KMS composite liquid Ⅲ was used and maintained until the end of anesthesia. During anesthesia KMS composite fluid infusion rate was changed on the basis of the changes of patient heart rate, blood pressure,tears and other. （2）Comparison of four different KMS anesthesia was performed.Results　（1）All cases of this study had rapid induction and stable anesthesia. the SBP, MAP and HR did not have significant difference at each time point(P>0.05). The follow-up in this group during the first 24～78 postoperative hours showed the satisfactory effects,such as improved pain relief, no consciousness and smooth awaking.（2） The clinical comparative study of four different KMS anesthesia method showed that: ①There was no significant difference between magnesium-fentanyl and procaine-fentanyl in SBP, DBP, HR, BG, ACTH, Cortsol, HGH during anesthesia(P>0.05). ②A comparative study between KMS intravenous anesthesia and fentanyl-enflurane inhalation-intravenous anesthesia showed that SBP,DBP and HR had no significant difference between two groups at each time point(P>0.05).③A comparative study between KMS intravenous anesthesia and fentanyl-enflurane anesthesia in the older patients showed that MAP,SV,SBP,HR and other indexes before and during endotracheal intubation in FE group significantly decreased than before anesthesia（P<0.05）,and these indexes were lower than KMS group at the corresponding time（P<0.5）.After anesthesia, the RPP of corresponding period in KMS group was lower than PE group（P<0.01）.④In the open heart surgery with beating heart, HR, SBP and DBP in KMS group were lower than those in the FE group. Intraoperative dosage of sodium nitroprusside in KMS group was less than that in FE group（P<0.01）;Postoperative dosage of dopamine in KMS group was less than that in FE group（P<0.05）. During the first 7 postoperative days, FE group appeared complications such as mental neurological symptoms, eye symptoms, severe arrhythmias, etc, but KMS group did not.Conclusion　KMS intravenous anesthesia method has lots of advantages, such as safe, rapid onset of anesthesia, less complications, exact curative of effect, stable anesthesia，stable circulation，smooth awaking，rare nausea, low prices, etc. This method can be widely used for variety of anesthesia cases and the age of surgical anesthesia.]]> 2018/10/8 8:28:53 LI Dan-ya, LIN Cheng-xin, ZHANG Bing-dong, et al. 915true ［Abstract］　Objective　To study the role of high molecular weight keratin (34βE12),CK19 and TPO protein in the differentiated diagnosis between thyroid papillary carcinoma and benign thyroid diseases.Methods　The expression of 34βE12, CK19 and TPO protein in 93 cases of papillary thyroid carcinoma and 102 cases of benign thyroid diseases(51 cases of benign thyroid papillary hyperplasia and 51 cases of thyroid follicular adenomas) as well as 50 cases of suspicious thyroid papillary carcinoma in histopathology were detected by inmmunohistochemistrical EnVision method.Results　Of the 93 cases of thyroid papillary carcinoma, the positive rates of 34βE12, CK19 and TPO were 90.32%, 100% and 8.6%, respectively;while all of the 51 cases of thyroid benign papillary hyperplasia were negative to 34βE12 and positive to TPO, 17.6% of them were positive to CK19;of the 51 cases of thyroid follicular adenomas, the positive rates of 34βE12, CK19 and TPO were 1.96%、33.33%、88.24%；respectively;and of the 50 cases of suspicious thyroid papillary carcinoma in histopathology, the positive rates of 34βE12, CK19 and TPO were 12%、56%、98%,respectively. The assessed values of 34βE12, CK19 and TPO in the diagnosis of thyroid papillary carcinoma were below: the sensitivity were 90.32%, 100% and 91.4%，the specifity were 99.09%, 74.51% and 94.12%，the accuracy were 94.87%, 86.67% and 87.69%，respectively. Parallel test showed that when combined with 34βE12 and TPO, the sensitivity was improved to 99.4%, and the specifity was 93.6%.Conclusion　34βE12, CK19 and TPO proteins are useful markers in the differentiated diagnosis between thyroid papillary carcinoma and benign thyroid diseases.]]> 2018/10/8 8:28:53 ZHOU Xiang-zhen, MO Xiang-lan, HUANG Zhen-lu,et al. 914true ［Abstract］　Objective　To discuss the advantages of consolidated department of internal and surgical neurology in a stroke unit and investigate the short-term curative effects of organized stroke treatment model of hemorrhagic stroke.Methods　The departments of internal and surgical neurology were integrated into a one-level clinical administrative unit—Department of Neurology and Neurosurgery so as to establish a perfect and multi-subjects network of organized stroke treatment model of hemorrhagic stroke. Eighty-one patients who matched with the standard were randomized into two groups: organized stroke care group (Group A) and control group (Group B). Both groups were compared and analyzed in terms of the degree of nerval disfunction, the ability of daily life and activities, the degree of unconsciousness, the complication and the short-term clinical effects, and the consequent economic and social benefits were also analyzed.Results　The evaluations of nerval disfunction, the ability of daily life and activities, the degree of unconsciousness and the short-term clinical effects of group A were apparently better than those of group B (P<0.01), and the complications of group A less than those of group B (P<0.01). And also, each increase of a function unit in group A cost less than that of group B.Conclusion　Integrating the departments of internal and surgery neurology into a one-level clinical administrative unit has its advantages, and the organized stroke treatment model is effective in treating hemorrhagic stroke.]]> 2018/10/8 8:28:53 LI Ci-fa,WEN De-shu, ZHOU Tie-jun 913true ［Abstract］　Objective　To investigate the mechanism of Chinese medicine Buqi Jiedu Decoction in the treatment of childbearing aged women with pre-pregnant active TORCH infection.Methods　Thirty-nine childbearing aged women with pre-pregnancy TORCH-IgM-positive infection were selected as observed group other 20 TORCH-IgM-negative healthy women as control group, using ELISA to detect the level of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2) in serum of TORCH-IgM-positive patients before and after treatment and healthy women in the control group.Results　Before treatment,there was statistical significance in the differences of the level of TNF-α and IL-2 between TORCH patients and the control group（P<0.05）,the level of TNF-α in TORCH patients was higher than that in the control group, while the level of IL-2 in TORCH patients was lower than that in the control group. There was no statistical significance in the differences of the level of TNF-α and IL-2 between TORCH patients with clinical cure and the control groupe(P> 0.05).Conclusion　Buqi Jiedu Decoction can exert the role of anti-TORCH infection by enhancing immune function,it can make the level of TNF-α and IL-2 returned to normal levels in order to repair the body′s immune function.This may be the mechanism of Buqi Jiedu Decoction in active TORCH infection, it also provide a theoretical basis of Chinese medicine in clinical development for the treatment of TORCH infections.]]> 2018/10/8 8:28:53 OU Qi-wen,WANG Qiong,MO Ke-liang,et al. 912true ［Abstract］　Objective　To study the role of psychological care and support in treatment of oral lesion in HIV patients.Methods　One hundred and thirty HIV patients with oral lesion were randomly divided into experimental group and control group. Each group consisted of 65 patients. Antifungal treatment and some comprehensive treatments were introduced into each group,while a full-time psychological care and support was provided for each patient by professional staff in experimental group.Results　Treatment outcome was more effective in experimental group than control group.Conclusion　Patients compliance can be improved and a short treatment course can be attained with psychological care and support in treatment of oral lesion in HIV patients.]]> 2018/10/8 8:28:53 DENG Fu-qiu,HE Yan-ying,CHEN Yan-ping 911true ［Abstract］　Objective　To develop a productive,inexpensive and stable method for the isolation and culture of rat hepatic stellate cells (HSCs) as a cell model for the studies of hepatic fibrosis.Methods　Rat HSCs were isolated by liver perfusion with pronase and collagenase followed by density gradient centrifugation with Nycodenz. Results　The harvest rate of hepatic stellate cells was （3.2±0.67）×10⁷ cells per liver and viability rate was more than 90%.Conclusion　This method is an ideal option for the isolation and culture of rat HSCs.]]> 2018/8/27 8:16:11 ZHANG Guo, HUANG Jun-juan,ZHANG Fa-can, et al. 910true ［Abstract］　Objective　To study the early diagnosis value of urinary NHE3 in acute renal injury (AKI).Methods　One hundred critically ill patients hospitalized in intensive care unit were enrolled in the study. Thirty health people were included as the control group. Urinary and blood samples were collected daily.Serum creatinine was detected by enzymic method, urinary NHE3 was detected by ELISA. AKI was defined as the absolute increment over ≥26.4 μmol/L or 50% increased of serum creatinine from the baseline.Results　Twenty-eight of the 100 patients developed AKI. The baseline levels of serum creatinine and urinary NHE3 were similar in three groups. Urinary NHE3, Scr, GFR were no changed in non-AKI group. Urinary NHE3 in AKI group was significantly higher than baseline (P<0.05) 24 h before AKI diagnosed. According to the sensitivity and speciticity of NHE3,ROC curves were drawn：ROC analysis confirmed diagnostic accuracy for NHE3 in AKI, area covered under the AUC curve was 0.758(95%CI: 0.635～0.804, P<0.05). With the cut-of value of NHE3 increased≥50%, the diagnostic sensitivity and specificity in AKI were 67.9% and 88.9%, the positive predictive value and negative predictive value were 70.4% and 87.7% respectively.Conclusion　Urinary NHE3 can diagnose AKI earlier than Scr in ICU patients, which may be a good biomarker for early diagnosis of AKI.]]> 2018/8/27 8:16:11 ZHANG Wen-xin, GONG Zhi-feng, PENG Xiao-mei, et al. 909true ［Abstract］　Objective　To investigate the clinical application value of amniocentesis（AC） and fetal blood sampling（FBS）guided by color Doppler ultrasound in prenatal diagnosis.Methods　A total of 1 008 pregnant women with prenatal diagnosis indications were divided into two groups according to the pregnant weeks:AC group including 943 cases of pregnancy between 17 and 21 weeks, FBS group including 65 cases of pregnancy between 22 and 36 weeks. The amniotic fluid and fetal blood samples were adopted by color Doppler ultrasound-guided AC or FBS for genetic analysis and karyotyping.Results　In AC and FBS groups,the successful rate of first puncture was 100% and 61.5%, respectively. In AC group,the contamination of the maternal blood was 0.1%, 36 cases of abnormal chromosome and 16 cases of thalassemia major were found.Conclusion　Both AC and FBS guided by color Doppler ultrasound are safe and reliable, and they have a high successful rate. They are significant in screening for prenatal diagnosis.]]> 2018/8/27 8:16:11 XU Chun-mei, WAN Li-kai, YANG Jin-lan, et al. 908true ［Abstract］　Objective　To compare the effects and safety of ligasure vessel sealing system and bipolar electric coagulation in laparoscopic hysterectomy. Methods　The clinical data of 80 patients with myoma of uterus who had put laparoscopic hysterectomy were retrospectively analyzed. In 40 patients received laparoscopic hysterectomy, ligasure vessel sealing system was applied to occlude the ovarian and uterine vessels, in another 40 patients used bipolar electric coagulation. The operation time,intra-operative blood loss, recovery condition after the operation and postoperative early complications were compared.Results　Both ligasure and bipolar electric coagulation could effectively coagulated the vessels smaller than 3mm, their operation time and intra-operative blood loss were similar. But there were significant differences between two methods in the occlusion effects on ovarian and uterine vessels whose diameters were 3～5 mm.Both of them recovery condition after the operation and postoperative early complications were similar.Conclusion　Ligasure and bipolar electric coagulation could effectively coagulate the vessels whose diameters are<3 mm.Ligasure can occlude the large vessel (3～5 mm) more safely and effectively than bipolar electric coagulation. Ligasure can reduce the intra-operative blood loss and shorten the operation time.]]> 2018/8/27 8:16:11 YANG Liu, CHEN Chang-Yi 907true ［Abstract］　Objective　To explore the mechanism and effect on treatment of nonunion of limbs fracture after internal fixation with extracorporeal shock wave. Methods　Twenty-one cases of 20 patients were treated with surgical operation.Among them,6 cases were treated with intramedullary nailing; 8 cases were treated with plate; 4 cases were treated with memory alloy embracing fixator; 3 cases were treated with external fixator. There were 2 cases of ulnar fracture,4 cases of humeral fracture, 6 cases of femoral fracture and 9 cases of tibial fracture.All cases were treated 2～3 times with extracorporeal shock wave.Results　All cases were followed up.Sixteen cases were cured with extracorporeal shock wave. In 5 cases who had not bone growth after 3 months,2 cases were cured with extracorporeal shock wave again.18 case of all patients were cured with extracorporeal shock wave.Cure rates of them were 85.7%.There were 2 cases of skin injury.There were not damage of nerve and blood vessel.Conclusion　The technique of extracorporeal shock wave has advantages of minimally invasive,less complications,low expenses,no hospitalization and true effects.It could promot the bone healing. We could acquire satisfied curative effects in treatment of nonunion of limbs fracture after internal fixation with extracorporeal shock wave.]]> 2018/8/27 8:16:11 LI Hong-yu,LIANG Bin,LI Li-chun,et al. 906true ［Abstract］　Objective　To research the value of fragmented QRS complex in the diagnosis of acute myocardial infarction(AMI).Methods　Three hundred and eighty patients who were diagnosed as coronary heart disease through coronary angiography were analyzed retrospectively. Specially, the incidence rate, sensitivity, specificity, positive predictive value and negative predictive value in AMI between fQRS and Q-wave were compared.Results　In diagnosis of AMI, sensitivity of fQRS was slightly lower than Q-wave′s. However, specificity and positive predictive value of fQRS were higher than Q-wave′s.Conclusion　With other evidences for AMI diagnosis, fQRS also could be considered as a new indicator. Furthermore, it may be a signal of non-transmural myocardial infarction.]]> 2018/8/27 8:16:11 LIANG Fang, ZHANG Wei, GU Ping, et al. 905true ［Abstract］　Objective　To investigate a method for cut needle biopsy for parapharyngeal infratemporal area neoplasms.Methods　Cut needle biopsy was performed on 4 cases of parapharyngeal infratemporal area neoplasms,with automatic biopsy gun.Results　All reached the right positions,all had the right qualitative diagnosis,and no complications were produced.Conclusion　Cut needle biopsy of parapharyngeal infratemporal area neoplasms has a high success rate and diagnostic accuracy rate with little complication.It is worth being clinically applied.]]> 2018/8/27 8:16:11 LI Min,SI Yong-fen,QIN Yang-da,et al. 904true ［Abstract］　Objective　To investigate the therapeutic effect of perceptual learning in pre-school children with ametropia.Methods　A randomised, self-controlled experiment. Fifty-three pre-school children with ametropia (male 28 cases, female 25 cases) aged (5.4±0.9) years old, were enrolled in visual science and optometry center between January 2009 and March 2010. And original best corrected visual acuity(BCVA) ranged from 0.3 to 0.1 in LogMAR chart. All subjects were corrected spectacles and underwent perceptual learning for no less than 5 months. All of them were followed in each month and BCVA were recorded in LogMAR visual acuity. BCVA were compared before and after perceptual learning by variance analysis (SPSS 13.0 version).Results　(1)BCVA of the 53 pre-school children with ametropia before and after perceptual learning were (0.163±0.068)LogMAR,(0.079±0.066)LogMAR,(0.060±0.063)LogMAR,(0.042±0.055)LogMAR,(0.037±0.056) LogMAR, (0.028±0.047)LogMAR, respectively. (2)Statistical outcomes showed that BCVA in each month after perceptual learning were improved significantly compared with BCVA before perceptual learning (F=164.655,P<0.01 ). (3)LSD showed that BCVA in 1-, 2-, and 3-month were different from each other(P<0.01), but no significant difference between 3- and 4-month after perceptual learning(P=0.167). However, significant difference was showed between 4- and 5-month after perceptual learning(P<0.05).Conclusion　Perceptual learning could improve BCVA of pre-school children with ametropia in a short term.]]> 2018/8/27 8:16:11 ZHAO Wu-xiao, LIU Wei-min, XIAO Xin, et al. 903true ［Abstract］　Objective　To study the porcine beating heart model for heart preservation, review the meaning and value.Methods　Twelve Bama pigs weighted 25～30 kg were chosen,6 pigs were only used for sampling blood, hearts of another 6 pigs were used for donor heart models.Pigs underwent chest incision under anesthesia and general heparinication(4 mg/kg).Then 500 ml blood were removed from the superior vena cava. Blocked and cut off the brachiocephalic anteries,inserted perfusion tube and cut off the superior vena cava and right superior pulmonary,then vein blood cardioplegic solution infusion was performed for cardiac arrest. Cut off the beginning part of the descending aorta and the two branches, the left ventricular drainage tube was insorted into removed heart via the pulmonary vein and opening of mitral valve. A device with continuous perfusion with oxygenated warm perfusion of blood was used for maintaining empty heart jump 8 h.Results　Six hearts were well preserved for 8 hours. In addition to 1 case of atrial conduction block (2∶1) in a brief period, the others didn't be found arrhythmia. Hematocrit from the perfusion 1 hour (T₁) to 5 hours (T₅) increased gradually, gradually decreased from T₇ to T₈; free calcium and potassium ions are gradually increasing trend; pH and BE have gradually decreased. Save beating heart perfusion pressure from T₁ to T₄ decreased perfusion pressure, then went in a relatively stable value. Coronary venous oxygen tension was (56.62 ± 6.93)mmHg. Heart surface had been found without drying or edema.Conclusion　“Bama miniature pig” is ideal for cardiovascular disease research laboratory animal, in vitro porcine model of heart beating donor heart preservation for transplantation research has more important meaning and value.]]> 2018/8/27 8:24:30 LIN Hui, MO An-sheng, WEN Zhao-ke, et al. 902true ［Abstract］　Objective　To investigate the optimal insulin regimens in rabbit diabetes mellitus model according to changes of the nephridial tissue,expression of the inflammatory factors and the components of extracellular matrix(ECM) in kidney.Methods　Fourty male rabbits were randomly divided into N group; D group (diabetes mellitus model group); A group received multiple injection of insulin; B group pre-mixed 50R; C group pre-mixed 30R. The animals of treated groups with insulin therapy were sacrificed 30 days later. The animals of other groups were sacrificed at the same time. The insulin dose and period at the targeted blood glucose level among 3 insulin groups was recorded. The blood glucose levels, HbAlc, daytime mean blood glucose(MBG),blood glucose mean of standard deviation(MOS), each rabbit blood glucose mean of definite difference(MODD),NW,TNF-α,CIV,LN,PCⅢ were observed and the expression of NF-κB in the nephridial tissue analysed.Results　The period was longest and the amount of insulin was the largest in C group at the targeted blood glucose level(P<0.01). The NF-κB gradation value of D group and A group were significantly lower, and TNF-α,CIV,LN,PCⅢ of D group and A group were markedly higher than those of C and N group(P<0.05). MOS and MODD were significantly higher in A group than those in C and N groups (P<0.001); HbAlc was positively correlated with MBG(P<0.001). However, it was non-related with MOS and MODD. The blood glucose were negatively related to the NF-κB gradation value;were positively related to TNF-α(P<0.02) in D group. The severity damage on kidney and expression of the inflammatory factors in modle group and every treated group were D>A> B> C.Conclusion　Pre-mixed 30R may be the optimal insulin treatment protocols.Insulin therapy by multiple injection and reaching the target blood glucose quickly should not be used as a primary regimen.]]> 2018/8/27 8:24:30 LI Ni, ZHONG Mei, CHEN Hui, et al. 901true ［Abstract］　Objective　To explore the pharmacological effects of epimedium pubescen flavonoid(EPF) on the bone mineral density(BMD), the biochemical markers of bone metabolism in the passive smoking rats, and the correlations between BMD and the biochemical markers. Methods　Sixty Sprague-Dawley(SD) rats(aged 2-month-old) were randomly divided into six groups(n=10), including sham control group(group A), model group(group B), control group(group C), treated 1 group(group D), treated 2 group(group E) and treated 3 group(group F). There were five male rats and five female rats in each group, and the male and female rats had been fed in different cage. All the rats were given the same feedstuff. Meanwhile, group C were given calcium 75 mg·kg^-1·d^-1 combined with WitD₃ 21 IU·kg^-1·d^-1 by gastrogavage for 4 months; group D EPF 75 mg·kg^-1·d^-1; group E EPF 150 mg·kg^-1·d^-1; group F EPF 300 mg·kg^-1·d^-1. Except the sham control group(group A), the other groups had been exposed daily to passive cigarette smoking for four months basing on the adytum fuming method. The experimental rats′ urine in 24 hours had been collected through the metabolize cage means and the vein blood had been collected through to cut off the rats′ trail at the end of the eighth weeks and the fourth months. These had been measured i. e. (1)The serum Ca, serum P, serum ALP, urine Ca, urine P, and urine Hop at the end of the eighth week and the fourth month; (2)The Serum BGP, Serum TRACP, Urine Dpd/Cr; (3) the BMD of the femur and lumbar vertebra body. The correlations had been analyzed between the BMD and the biochemical markers of the bone formation and resorption. Results　(1)The BMD of the femur and lumbar vertebra body, the serum BGP(ng/ml), the serum ALP at the end of the eighth weeks and at the end of the fourth months had increased markedly in group A,group C, group D, group E and group F compared with group B, and the difference between group A, group C, group D, group E, group F and group B had statistical significance (P<0.05). There had dose-dependently increase in group D, group E and group F, and the difference among them had no statistical significance (P>0.05). The difference had no statistical significance (P>0.05) among the other groups. The serum TRACP(U/L) and urine Dpd/Cr(nmol/mmol) had decreased markedly in group A, group C, group D, group E and group F compared with group B, and the difference between group A, group C, group D, group E, group F and group B had statistical significance (P<0.05). There had dose-dependently decreased in group D, group E and group F, and the difference among them had no statistical significance (P>0.05). The difference had no statistical significance (P>0.05) among the other groups; (2) The urine Ca and urine Hop at the end of the fourth months and at the end of the eighth weeks, the serum TRACP and urine Dpd/Cr had decreased markedly in group A,group C, group D, group E and group F compared with group B, and the difference between group A, group C, group D, group E, group F and group B had statistical significance (P<0.05). There had dose-dependently decreased in group D, group E and group F, and the difference among them had no statistical significance(P>0.05). The difference had no statistical significance (P>0.05) among the other groups. The serum ALP had increased markedly in group A, group C, group D, group E and group F compared with group B, and the difference between group A, group C, group D, group E, group F and group B had statistical significance (P<0.05) at the end of the eighth weeks and at the end of the fourth months. There had dose-dependently decreased in group D, group E and group F, and the difference among them had no statistical significance (P>0.05). The difference had no statistical significance (P>0.05) among the other groups. The difference of the other biochemical indices had no statistical significance (P>0.05) among the other groups. A positive correlation was found between the BMD of the femur and lumbar vertebra body and serum BGP, serum ALP (r=0.784, 0.816, P<0.05； r=0.743, 0.807, P<0.05). There were also a negative correlation between the BMD of the femur and lumbar vertebra body and serum TRACP, Urine Dpd/Cr, urine Ca, urine Hop(r=-0.617, -0.608, -0.587, -0.611, P<0.05； r=-0.614, -0.621, -0.583, -0.617, P<0.05). Conclusion　The EPF could prevent and treat effectively the decreasing of BMD and the worsening of biochemical markers of bone metabolism resulted from passive smoking in the experimental rats. There are significant correlations between the BMD and the biochemical markers of the bone formation and resorption.]]> 2018/8/27 8:24:30 LEI Guang-hua,LIU Wen-he,GAO Shu-guang 900true ［Abstract］　Objective　To investigate the correlation of carotid arteriostenosis and atheromatous plaques with cerebral infarction.Methods　Carotid artery was assessed using Doppler ultrasongraphy in 32 patients with cerebral infarction and in 32 patients without cerebral infarction, and the characteristics of carotid artery were compared between patients with or without cerebral infarction.Results　Of 32 patients with cerebral infarction, sixty-seven atheromatous plaques were detected in 30 patients(93.75%), and the carotid arteriostenosis was found in 23 patients（71.88%）.The mild, moderate, severe carotid arteriostenosis and occlusion were found in 3 patients（9.38%）,12 patients（37.50%） and 4 patients（12.50%）, respectively. Of 32 patients without cerebral infarction, twenty-two atheromatous plaques were detected in 12 patients(37.50%).The mild carotid arteriostenosis was found in 2 patients(6.25%), a significant difference (P<0.01) in carotid arteriostenosis and atheromatous plaques between two groups.Conclusion　Carotid arteriostenosis was participated in cerebral infarction. Doppler ultrasongraphy shows a relationship between atheromatous plaques and cerebral infarction. Using Doppler ultrasongraphy can show lesions of carotid arteriostenosis which is very important in preventing cerebral infarction.]]> 2018/8/27 8:24:30 ZHONG Wei-zhang, WANG Xiao-yan, LI Lv -li,et al. 899true ［Abstract］　Objective　To investigate the relationship between the stage of root development and the root resorption during orthodontic treatment.Methods　One hundred and twelve patients (32adult, 80 adolescents) treated by straight wire appliances were selected.The adolescents were subdivided into the developing group and the developed group according to the stage of root development at the beginning of the treatment.The stage of root development and the level of root resorption of 448 premolars were detected in the panoramic radiographs before and after the orthodontic treatment.The data were analysed by SPSS11.0 software package.Results　The roots of most of the teeth in developing group continued to develop during orthodontic treatment and the shape of root was normal with no resorption after treatment.Root resorption occured in developed group and adult group after orthodontic treatment with different proportion.The score of root resorption among three groups differed significantly (P<0.01), whereas the difference of root resorption between the developed group and adult group after orthodontic treatment was not found (P>0.05) and most of the root resorption were slight.Conclusion　The stage of root development at the beginning of treatment have significant influence on the root resorption during orthodontic treatment.Not only the adult but also the adolescent suffer the same susceptibility to root resorption in the orthodontic treatment once the root was developed,but the level of root resorption is slight.]]> 2018/8/27 8:24:30 FANG Zhi-xin,ZHOU Yan,CHEN Shi-wen, et al. 898true ［Abstract］　Objective　To study the cognitive function evaluation in children with learning difficulties through Das-Naglieri cognitive assessment system (DN：CAS), Raven′s Progressive Matrices (Combined Raven Test, CRT) and the Tower of Hanoi. Methods　The cognitive function of 342 children (first, third and fifth grade primary school students who were 104, 92, 146 respectively) were evaluated by DN：CAS, CRT and the Tower of Hanoi and were measured at Maths and Chinese with unified classified standardized test. The data of children including gender, age, school, grade, parent occupation, and parental education were collected. 10% of the poorest achievement in Maths, in Chinese, in both Maths and Chinese were named the students with Maths learning difficulties(MLD), Chinese learning difficulties(CLD), double learning difficulties(DLD) respectively. Results of the three kinds of cognitive functioning measurements and the relevant data as the impact factors of children′s Maths and Chinese test were analyzed by univariate, multivariate logistic regression, the goodness of fit and likelihood ratio respectively. Results　（1）Univariate analysis results of the Tower of Hanoi were not associated with learning difficulties in all types of students, and DN:CAS and CRT results were associated with learning difficulties in all types of students. （2）Multiple logistic regression analysis showed DN:CAS and CRT results were associated with learning difficulties in all types of students. （3）Goodness of fit analysis showed the prediction accuracy of DN:CAS and CRT results on MLD, CLD and DLD were 45.2%, 55.2%, 60.0% and 32.2%, 34.5%, 46.7% respectively. （4） Likelihood ratio analysis showed positive likelihood ratio (LR+) of DN：CAS and CRT on MLD, CLD and DLD were 0.81, 1.23, 1.5 and 0.48, 0.53, 0.88 respectively, and negative likelihood ratio(LR-) were 141.9, 70.42, 141.86 and 54.56, 39, 99 respectively. Conclusion　DN：CAS is more precise than CRT on the primary school students′ cognitive function evaluation.]]> 2018/8/27 8:24:30 QIN Ling, DENG Ci-ping,WU Xin,et al. 897true ［Abstract］　Objective　To explore the efficacy and safety of the specific sublingual immunotherapy of dermatophagoides farinae drops in children sensitized to many kinds anaphylactogen with stable asthma and allergic rhinitis.Methods　291 cases of children sensitized to dermatophagoides farinae in four to thirteen years old with asthma in remission stage and allergic rhinitis were assigned into four groups according to the results of skin prick test: only sensitized to mites(eighty cases), sensitized to mites and cockroach(seventy-one cases), sensitized to mites and pollen(seventy-four cases), and sensitized to mites and dog wool(sixty-six cases). Skin prick test with ten common anaphylactogen including mites were taken in children and positive reaction to anaphylactogen was confirmed. Lung functions in all children were in predicted value scope. “Dermatophagoides farinae drops（DFD）” provided by Wolwo Biotech Co, Ltd. in Zhejiang province.be used for sublingual immunotherapy. Clinic symptom score before and after treatment and side reaction were recorded.Results　The clinical effective rate of desensitization with DFD after one year the sublingual immunotherapy in children with asthma and allergic rhinitis was 62.5%. Among them, group sensitized to mites was 53.8%, group sensitized to mites and cockroach were 66.2%, group sensitized to mites and pollen were 70.3%, group sensitized to mites and dog wool were 60.6%. There was no statistically significant between two groups on clinic effective rate of immunotherapy. The incidences of side reaction related to immunotherapy including skin rash and nasopharynx itches and asthma attack was 23.67%, while no anaphylactic shock happened. Conclusion　Sublingual immunotherapy method with dermatophagoides farinae drops was obvious clinically safe and effective for children with asthma and rhinitis, not only for those only sensitive to dermatophagoides farinae, also dermatophagoides farinae accompany with other anaphylactogen.]]> 2018/8/27 8:24:30 XIE Qing-ling, ZHEN Hong, HU Qiong-yan, et al. 896true ［Abstract］　Objective　To analyse the long-term effect of interleukin 2(IL-2) combined with radiochemotherapy for treating advanced nasopharyngeal carcinoma. Methods　Ninety patients with advanced nasopharyngeal carcinoma(NPC) were divided into 3 groups randomly. A group was treated by combination of local IL-2 injection with radiochemotherapy, B group was treated by combination of IL-2 systemic administration with radiochemotherapy, C group was treated by radiochemotherapy. The five-year survival rate and ten-year survival rate were observed. Results　Five-year survival rate in B group (70%) were superior to those in C group (36.7%) (P<0.05) and five-year survival rate in A group (63.3%) were superior to those in C group (36.7%) (P<0.05); the ten-year survival rates in B group （50%） were superior to C group （23.3%） (P<0.05), but there was no difference of ten-year survival rates between A group and B group (P>0.05). Conclusion　Combination of Interleukin 2 with radiochemotherapy can improve the survival rate of advanced NPC patient. Systemic administration of IL-2 has better effect than local administration of IL-2 for treating advanced NPC.]]> 2018/8/27 8:24:30 ZHANG Zheng, QIN Ying,WENG Jing-jin, et al. 895true ［Abstract］　Objective　To observe and collect the information of determination of bladder cancer stem cell marker CD44 and CXCR4.Methods　Cell line is bladder cancer 5637 cell line. The different environments (niche) include cell culture system, the model of human bladder cancer xenograft, the metastatic lymph node model of bladder cancer. CD44 and CXCR4 were detected by immunohistochemical staining and flow cytometry respectively.Results　In the bladder cancer 5637 cell line in vitro, CD44 was strong positive, and CXCR4 was low positive. In the model of xenograft, CD44 and CXCR4 were positive. Compared with the bladder cancer 5637 cell line in vitro, the positive expression of CD44 was no change and the positive expression of CXCR4 was higher (P<0.01). In the metastatic lymph node model, CD44 and CXCR4 were positive.Conclusion　The expression of bladder cancer stem cell markers are regulated by the different environments. Bladder cancer stem cells exist in the 5673 cell line.]]> 2018/8/31 16:02:30 CHENG Ji-wen, MENG Qing-gui, TANG Yong, et al. 894true ［Abstract］　Objective　To observe the effect of rhinallergosis oral liquid on marrow IL-5 in rat model of allergic rhinitis,in order to discuss the mechanism of that remedy.Methods　Forty rats were divided randomly into rhinallergosis oral liquid experiment group,chlorpheniramine control group, saline control group and normal control group;the next day after the model was fulfilled,rhinallergosis oral liquid, chlorpheniramine and saline were separately lavaged to the stomach of rats in former 3 groups for 7 days;then rats were sacrificed, the skeleton marrow of them was got,then centrifugation and smear were performed,and the change of marrow IL-5 was observed by the method of ELISA.Results　Compared with chlorpheniramine control group and saline control group,the marrow IL-5 of rhinallergosis oral liquid experiment group were better(P<0.01),but there were no statistical significant difference compared with normal control group(P>0.05).Conclusion　Rhinallergosis oral liquid can decrease the marrow IL-5,then can control AR,this could be one of the mechanism of the therapy of rhinallergosis oral liquid in the treatment of AR.]]> 2018/8/31 16:02:30 FENG Wei-yun，WU Yan-jia，WEI Chang-li，et al. 893true ［Abstract］　Objective　To evaluate the effect of different anaesthetics on lung injury in rabbits subjected to pneumoperitoneum.Methods　Fifty rabbits were randomly assigned to following groups according to different anaeathetics.Group(P1) and group (P2) received propofol,group (Pen) received pentobarbital sodium, group (M+F ) received midazolam and fentanyl,group (K) received ketamine. Group (P1) was subjected to a sham pneumoperitoneum and the other rabbits were subjected to 3 hours pneumoperitoneum. After 20 min of desufflation subsequent to cessation of pneumoperitoneum, blood, lung, and bronchoalveolar lavage fluid (BALF) samples were obtained，white blood cell(WBC) count in BALF were determined.The myleopemxidase (MPO) activity and wet to dry weight ratio(W/D)were also measured.Results　The mortality rate of group (Pen) was the highest in all groups(P<0.01). Lung permeability index(LPI), WBC count in BALF and the lung tissue MPO activity had no difference in either two groups(P>0.05) except group (P1)(P<0.01).Conclusion　It seemed that pentobarbital sodium as an anaesthetics by intravenous injection is not suitable for rabbit compared with the other three anaeathetics,but there′s no difference in the effect on lung injury among the four groups.]]> 2018/8/31 16:02:30 BAO Yan-li,HUANG Zhong-hua, NING Jia-juan,et al. 892true ［Abstract］　Objective　To explore a quick and effective method for the establishment of rat model of Parkinson disease.Methods　A total of 92 Wistar rats were randomly divided into experimental group(n=80) and control group(n=12)，6-OHDA was injected into rats′ dextral midbrain above the ventral tegmental area(VTA) by stereotaetie technology and apomorphine(APO) was given by intraperitoneal injection on 1，2，3 and 4 weeks after 6-OHDA injection.The rat which showed consistently left whirling and whose numbers of rotation were more than 210 r/30 min induced by APO was judged as PD model.The contral group was injected with saline instead of 6-OHDA.The morphological changes and the number of tyrosine hydroxylase(TH) positive neurons were detected by immunohistoehemistry.Results　(1)One rat in the control group and 2 rats in the model group died respectively after the operation.Behavioral test showed that there were 36 from 78 rats regarded as successful PD models whose numbers of rotation were more than 210 r/30 minutes after operation and the rotation was stable,the successful ratio was 46.2%.(2)TH positive neurons of injury VTA in model group were less than those in the opposite side and in control group obviously(P<0.01).Conclusion　The one-spot injection method for PD is more convenient and practical with lower mortality of animal and higher successful rate of model establishment，the cost is more low.The stable PD rat models can soon be established.]]> 2018/8/31 16:02:30 PAN Xiao-rong，HU Yu-ying，FENG Dao-rong，et al. 891true ［Abstract］　Objective　To investigate the diagnostic information of umbilical cord blood available and increase ultrasonic test facility. Methods　The direction of blood flow may be known according to color Doppler. The fetal umbilical cord, fetal position, placenta position and umbilicus artery afflux were observed in order to get the informations of umbilical cord courser and judge the winding directions, and twining angle. Results　In 214 fetuses, one circle of the nuchal umbilical cord were found in 189 fetuses, two circles in 15 fetuses and three circles in one fetus. There were two different types according to the winding directions: type from left to right (n=102)a nd type from right to left (n=103), and four different types according to the twining angle: type“C” (n=76), type“O” (n=39), type“α” (n=90), and type“L”(n=9). Conclusion　The investigation indicates that the winding directions of nuchal umbilical cord may be judged correctly according to the cord blood flow vector. The typing according to the twining angle can reflect the twining degree of nuchal umbilical cord directly and finely. So, more diagnostic informations will be supplied for clinic.]]> 2018/8/31 16:02:30 CHEN Yan-hong,WAN Li-kai, XU Chun-mei, et al. 890true ［Abstract］　Objective　To investigate the impact of early hemodialysis on cardio-cerebral vascular events,the quality of life in the patients with diabetic nephropathy.Methods　Sixty-eight patients with diabetic nephropathy treated in the center of blood purification of Guangxi in the period of March 2005 to March 2009, with edema, blood pressure and blood glucose under good control, creatinine clearance rate (Ccr ) with a range of 15ml/min to 30ml/min), were randomly divided into 2 groups, thirty-three patients of the observation group were treated immediately by hemodialysis for 4.5 hours each time, three times a week, maintained for 2 years; thirty-five patients of the control group treated with conservative treatment begin to enter hemodialysis until the Ccr decline to below 15ml/min.Hemodialysis were performed for 4.5 hours each time, three times a week, maintained for 2 years. The cardiovascular and cerebrovascular events of two groups were observed from beginning of entering group to the end of 2 years of hemodialysis, included the occurrence and development of heart failure, angina, myocardial infarction, sudden cardiac death, malignant arrhythmia, cerebral vascular disease and hypertension.Results　The cardio-cerebral vascular events and the occurrence of hypertension in the observation group were significantly lower than those in the control group (P<0.01).There were a significant difference in the incidence of more than two kinds of cardio-cerebral vascular events and accumulated more than 4 times in the same patient of the two groups(P<0.05);Heart failure is the major cardiovascular events, followed by ischemic heart disease; There was no significant difference in mortality in the two groups (P>0.05);There were a significant difference in renal function and serum albumin (ALB) in the two groups before and after treatment (P<0.01), but blood glucose had no significant difference (P>0.05). While in the control group, the ALB at the beginning of entering into hemodialysis was significantly lower than both of at the time that patients entaned into the group and 2 years after hemodialysis (P<0.01); There were no significant difference in renal function, ALB and blood glucose in the two groups at the end of the study (P>0.05).Conclusion　For the diabetic nephropathy patients with edema complication, as soon as Ccr ≤30 ml/min, they should be intervened in advance by the maintenance of hemodialysis which can effectively reduce the incidence of cardio-cerebral vascular events and improve quality of life of diabetic nephropathy patients; Although early hemodialysis could not delay the progress of renal damage, but it can significantly improve the situation of hypoproteinemia.]]> 2018/8/31 16:02:30 BI Min, TANG Sheng,ZENG Chun,et al. 889true ［Abstract］　Objective　To analyze the correlations of refractive power and wavefront aberrations in anisomyopes by WaveScan WaveFront^TM System.Methods　Sixty anisomyopic patients(male 22, female 38), aged 8 to 40 years old(mean 21.2±7.4), in vision science and optometry center of Guangxi from August 2009 to February 2010, were enrolled in this study and wavefront aberrations in each eyes were captured for analyzation. And more myopic eyes with spherical equivalent (-4.81±1.79) DS, less myopic eyes with spherical equivalent (-2.44±1.78) DS. The outcomes included high order aberrations, coma, trefoil and spherical aberration. Pearson correlations between spherical equivalent and high order aberrations, coma, trefoil and spherical aberration were analyzed by SPSS13.0, respectively.Results　High order aberrations in more myopic eyes and less myopic eyes were (0.37±0.15)μm and (0.37±0.14)μm, respectively. Coma were (0.21±0.14)μm and (0.19±0.11)μm, respectively. Trefoil were (0.16±0.10)μm and (0.17±0.12)μm, respectively. Spherical aberration were (0.11±0.14)μm and (0.12±0.17)μm, respectively. Pearson correlations showed that there were low correlations between spherical equivalent and high order aberrations in more myopic eyes(r=-0.275, P<0.033), but there were no correlation among other indexes(P>0.05).Conclusion　Low correlations existed between spherical equivalent and high order aberraions in more myopic eyes of anisomyopia.]]> 2018/8/31 16:02:30 HUANG Jian-zhong, LIU Wei-min, Zhao Wu-xiao, et al. 888true ［Abstract］　Objective　To detect the mRNA expression of the cancer testis antigens (NY-SAR-35) in retinoblastoma (RB) and investigate the possibility of applying these antigens as the target antigens for RB specific immunotherapy. Methods　With reverse-transcription polymerase chain reaction (RT-PCR), the mRNA expression of NY-SAR-35 in 15 cases of RB tissues, 12 cases of nontumorous′ retina tissues and 22 cases of other ocular region normal tissues(12 cases of blepharo-cutis, 5 cases of musculi oculi and 5 cases of conjunctiva) was detected. Samples selected randomly from PCR positive products of each CT gene were directly sequenced. The results of expression and related clinical parameters(sex, age, patho-typing, tumorous size, clinical stage) were analyzed with statistic analysis software. Results　Among 15 RB tissues, NY-SAR-35 mRNA was detected in 60%（95%CI： 35.2%～84.8%）, and NY-SAR-35 mRNA can not be detected in both nontumorous′ region and other ocular region normal tissues. Conclusion　NY-SAR-35 is expressed specifically in RB. So NY-SAR-35 may become special target antigen of immunity treatment. This result is worth further study.]]> 2018/7/16 16:35:42 KONG Min,LIN Quan,LIANG Hao,et al. 887true ［Abstract］　Objective　To investigate the curative effects of antithyroid drug (ATD) on the patients with primary hyperthyroidism (PHT) of different imaging. Methods　Sixty-two patients with PHT of different imaging ［homogeneous echo (n=31) and inhomogeneous echo (n=31) ］ were randomly chosen and were treated with ATD. The volume of thyroid gland (V) was examined by using B-ultrasound and systolic and diastolic blood flow velocity (Vmax, Vmin) and resistance index (RI) were examined by using CDFI in these two groups. Serum levels of TT₃, TT₄, FT₃, FT₄, TSH, TRH, TRAb, TGAb and TMAb were also assayed before and after treatment. Results　There was no significant difference in V, Vmax, Vmin, RI, or FT₃, FT₄, TSH, TRAb before treatment（P>0.05）, but significant difference after treatment in the curative effects between the two groups (P<0.05）. The curative effects in homogeneous echo group was better than that in inhomogeneous echo group (P<0.05）. Conclusion　ATD treatment has good curative effects on the patients with PHT of homogeneous echo,but bad curative effects on those of inhomogeneous echo. It is very important in treatment.]]> 2018/7/16 16:35:42 XU Chun-mei, ZHU Shang-yong, LIU Hong-yan,et al. 886true ［Abstract］　Objective　To explore the mode of ventilation and the feasibility of it about using oxygen therapy devices made by oneself, in the case of no ventilator in large hyperbaric oxygen chamber, by comparing the treatment effectiveness of lung infection with severe brain injury with tracheotomy between hyperbaric oxygen and normal oxygen pressure. Methods　Through stratified by age, gender, GCS scores, 60 patients of lung infection with severe brain injury with tracheotomy were randomly divided into experimental group(n=30) and control group(n=30). Both groups were given reduced the intracranial hypotension, protected the brain, surgery, sensitive antibiotics, symptomatic and support therapy, oxygen therapy. In control group, under normal oxygen pressure, the patients received oxygen therapy by tracheal cannula. In experimental group, the patients received hyperbaric oxygen therapy by oxygen devices made by oneself. The body temperature, cough and expectoration, breath sounds, WBC were compared between the two groups.Results　There were statistical difference (P<0.01) in the body temperature, cough and expectoration, breath sounds, the total number of WBC, the time about normal category of WBC between experimental and control group. Conclusion　Oxygen therapy devices made by oneself can successfully complete treatment on lung infection with severe brain injury with tracheotomy by hyperbaric oxygen, in the case of no ventilator in large hyperbaric oxygen chamber. They met the mode of ventilation about positive expiratory end pressure, and have a higher value.]]> 2018/7/16 16:35:42 XU Li-min，KONG Lei，XIAO Quan，et al. 885true ［Abstract］　Objective　To observe the therapeutic efficacy of adefovir dipivoxil combined with lamivudine in the treatment of patients with chronic hepatitis B resistant to lamivudine. Methods　Eighty patients with chronic hepatitis B resistant to lamivudine (YMDD mutantion) were divided into two groups according to their own willings. Group A（control group, n=38） and B(observation group, n=42). Patients in group A were given adefovir dipivoxil 10 mg po per day for 132 weeks after a 12 weeks combined therapy of adefovir dipivoxil 10 mg each day and lamivudine 100 mg each day. Patients in group B were subjected to combined treatment of adefovir dipivoxil 10 mg per day with lamivudine 100 mg per day for totally 144 weeks. Changes in serum HBV-DNA, HBV-M, liver functions, renal functions and blood routine test were dynamically monitored every 12 weeks. Results　At the end of the 144 weeks of treatment, the rates of negative sero conversion for HBV-DNA were 95.2% in group B and 78.9% in group A respectively. The rate of sera to turn negative for HBV-DNA in group B was significantly higher than that of patients in group A(P<0.05). The negative conversion rates of HBeAg showed no difference between two groups. The rate of liver function normalization as well as other organ functions under testing in group B was showed significantly higher than that in group A. Conclusion　Adefovir dipivoxil combined with lamivudine in the treatment of the patients with chronic hepatitis B resistant to lamivudine has better therapeutic effects.]]> 2018/7/16 16:35:42 JIN Hong,JIN Shui-zhong,XU Hong,et al. 884true ［Abstract］　Objective　To evaluate the clinical outcome of coralline hydroxyapatite combined with autogeneous bone taken from implantation bed in dental implant. Methods　32 patients(37 implants) were divided into two groups. Group A: Coralline hydroxyapatite was the only graft in the bone defect area in 27 implants (24 patients), covered with resorbable collagen membrane or titanium membrane; Group B: FRIOSR Trephine was used for the preparation of autogenenous bone pieces. Coralline hydroxyapatite and autogenous bone particles were mixed and placed in the bone defect area in 10 implants (8 patients), covered with barrier membrane. Ridge dimensions around the implants were clinically or radiographically measured in stageⅠor Ⅱoperation. Results　New bone formation was observed at stage Ⅱ operation performed at 4～6 months after stage Ⅰ in both groups. Most of the patients showed satisfactory bone regeneration effects. The satisfactory rate of bone regeneration effects in group A and group B was 85.19% and 100% separately. Conclusion　Using bone graft harvested from implantation bed could achieve an effective clinical outcome. It is minimally invasive operation and deserves popularization.]]> 2018/7/16 16:35:42 SHEN Ning,TANG Zeng-bin,LI Jun,et al. 883true ［Abstract］　Objective　To investigate the association between human leukocyte antigen DRB (HLA-DRB) gene polymorphisms and asthma in children. Methods　Eighty-four unrelated asthmatic individuals and one hundred and sixty-eight healthy controls without asthma and atopy were involved in the study. All asthmatics had their serum total IgE levels measured with UniCAP Pharmacia system, and skin-prick test with ten kinds of inhalant allergens were taken among the asthmatics. HLA oligonucleotide array was used to twenty-one gene frequencies of HLA-DR. Results　The frequencies of HLA-DRB1^*070X allele and HLA-DRB1^*11XX allele among the asthmatics were 2.98% and 13.69%, significantly higher than those in healthy controls (0.3% and 5.95%, χ²=6.915, P<0.05 and χ²=9.478, P<0.01)）, Odds ratios (Ors) were 10.57（95%CI: 1.215～91.986） and 2.79（95%CI: 1.429～5.449） respectively. HLA-DRB3(52)^*010Xallele were significantly decreased in asthmatics compared to healthy controls (13.99%, χ²=5.854, P<0.05), Ors were 0.429（95% CI:0.214～0.862）. Conclusion　HLA-DRB1^*070X allele and HLA-DRB1^*11XX allele were implicated in susceptibility to asthma, HLA-DRB3(52)^*010X allele might confer protection against asthma.]]> 2018/7/16 16:35:42 XIE Qing-ling,ZHEN Hong,QIN Lin 882true ［Abstract］　Objective　To construct a eukaryotic expression vector with human CYB5R2 gene, which is high frequency methylation in nasopharyngeal carcinoma(NPC). Methods　According to the sequence of the multiple clone sites of the vector pCMV-Tag3A and the sequence of CYB5R2 gene, a pair of primers were designed. RT-PCR amplification of CYB5R2 gene CDS from human testis tissue cDNA was achieved. Then, the fragment was cloned into T-A coloning vector. The recombined vector confirmed by PCR, sequencing and double digested by Sal I/BamH I to obtain the gene cDNA fragment, and subcloned into the multiple sites of pCMV-Tag3A vector. The recombined vector confirmed by PCR, sequencing and double digested by Sal I/BamH I and BamH I/EcoR V. The recombinant plasmid was transfected into HONE1 cells by FuGENE HD Transfection Reagent. The result and expression were examined using fluorescent microscopy and RT-PCR. Results　PCR, sequencing and restriction enzyme digestion analysis indicated that the eukaryotic vector pCMV-Tag3-CYB5R2-CDS was constructed successfully. The expression of vector was confirmed by fluorescent microscopy and RT-PCR. Conclusion　The eukaryotic expression vector pCMV-Tag3A with CDS of gene CYB5R2 is successfully constructed, and with efficient expression in HONE1 cells, which serve a good basis for futher study on the relationship of methylation CYB5R2 gene with NPC.]]> 2018/7/16 16:36:19 YU Na-na， DU Chun-ping，XIAO Xue, et al. 881true ［Abstract］　Objective　To study the basic situation of the acute poison in Guangxi. Methods　By the epidemiological study, 6 011 cases of all kinds of acute poison were collected from 63 hospitals during 2005～2009 in Guangxi. Results　The analysis on characteristics of the poisoning cases displayed the women and men accounted for 53. 11% and 46. 89% cases respectively. The age range of the cases(accounted for 68. 84%) were between 20 and 60 years, and they (accounted for 61. 02%) lived in rural areas. The analysis on the characteristics of poisoning process displayed the top three reasons which were accidental, suicidal, and mistakenly predatory accounted for 39. 42%, 31. 41%, 21. 57% respectively. The cases who were caused by poison entered the body through the mouth, breath, and skin contact accounted for 70. 89%, 20. 70%, 10. 48% respectively. And they were sporadic cases primarily accounted for 89. 32%. The analysis of the environment and time displayed the 79. 53% of poison occurred in home. The number of cases in 2009 was (54. 00%) higher than that in 2005. And the average of increasing numberes in every year during 2006～2009 year was 13. 50%. Conclusion　In addition to mostly analytical results being similar to that reported at home and abroad, the Guangxi′s poisoning cause and sites have outstanding characteristics, and the gradually increasing of poisoning cases in every year is obvious. For this reason, the question of the acute poisoning in Guangxi will be further studied.]]> 2018/7/16 16:36:19 GE Xian-min,JIANG Dong-fang,LIU Qing-hua 880true ［Abstract］　Objective　To study the effects of low-dose hydrocortisone on inflammatory response in the patients with septic shock and to evaluate the curative effect of low-dose hydrocortisone. Methods　Eighty patients with septic shock were divided randomly into the treatment group and the control group. Both groups were treated by normal medicine and the treatment group by additional injection of low-dose hydrocortisone. MAP, HR, cardiac index, PaO₂/FiO₂ were measured and recorded before (baseline) and at 0, 4, 12, 24, 48 h and 72 h after treatment. APACHEⅡ score, the levels of BLA and PCT were recorded before (baseline) and at 24 h, 48 h and 72 d after treatment. Incidence of 28 d mortality between the treatment group and the control group were compared. Results　The indexes of two groups were similar before treatment (P>0. 05). APACHEⅡ score, BLA and PCT were significantly decreased compared with the baseline values before the treatment (P<0. 05), the levels of APACHEⅡ score , PCT in treatment group were lower than those in control group at according time(P<0. 05), while there were no significant difference in 28 d mortality between the treatment group and the control group(P>0. 05). There were no association between cortisol levels and the 28 d mortality rate(P>0. 05). Conclusion　Low-dose hydrocortisone can inhibit inflammatory response in patients with septic shock, and provide a chance treating primary diseases in patients with septic shock.]]> 2018/7/16 16:36:19 LIN Yong-jun, XIONG Bin,LU Li-wen, et al. 879true ［Abstract］　Objective　To investigate the psychological and psychical factors and the social supports in the older patients with chronic obstructive pulmonary disease (COPD). Methods　Questionnaires were performed in 264 patients with COPD and 142 healthy volunteers using Symptom Checklist 90 (SCL-90) and Social Support Rating Scale (SSRS). Results　The data of somatization, obsessive-compulsiveness, anxiety, phobic anxiety and paranoid ideation, psychotism and the mean of positive factors of SCL-90 of the COPD group were significantly higher than those of the control group (P<0. 05 or P<0. 01). The SSRS results of subjective and objective supports and total score of social supports of the COPD group were much lower than those of the control group (P<0. 05 or P<0. 01). Conclusion　Our results suggest that the disordered mental conditions and lower social support in patients with COPD need psychosocial interventions in addition to medical care.]]> 2018/7/16 16:36:19 LIAO Wei-ping, ZHU Li-yang 878true ［Abstract］　Objective　To observe the effect of tamsulosin in the adjuvant therapy after the extracorporeal shock wave lithotripsy (ESWL) in the patients with middle and lower ureteral calculi. Methods　From December 2009 to July 2010, after ESWL, 510 patients with middle-lower ureteral calculi were divided into two groups: the experimental group and the control group. The experiment was conducted by double-blind method. And the experiment group was added tamsulosin based on the control group. The observation lasted for 20 days. Results　The stone-free rate of the experimental group was 84. 5%(191/226), that of the control group was 62. 3%(177/284). The stone-free rate of the experimental group was significantly higher than that of the control group（P<0. 01）. Conclusion　Compared with the control group, the stone-free rate of the tamsulosin group significantly increased, the re-broken incidence and the renal colic incidence after broken stone significantly reduced, with fewer side effects, and mild symptoms.]]> 2018/7/16 16:36:19 PANG Ye-xiao, LIAO Kang 877true ［Abstract］　Objective　To analyse the causes of middle ear barotrauma and find preventions by observing 300 patients who were first treated by HBO. Methods　Tympanic membrane were checked after 1^st, 2^nd and 3^rd HBO treatment; we also asked the patients if blocked ear, swollen ear and earache were occurred in the process of pressurizing and decompression. Results　Thirty-one patients (approximately 10. 3%) suffered from middle ear barotrauma. The causes included non-standard pressure regulated action, absolute increase in eustachian tube ventilation resistance, difference of the inherent tension of the eustachian tube structures. Middle ear barotrauma always occurs in pressurizing step, mostly in the first day. Patients whose educational degree are not so high and is absent-minded will be suffered. Conclusion　Safety education, demonstration teaching of regulating action and acceptance shall be carefully done before the patients being conveyed into oxygen tank; the patients also strictly act as doctor′s instructions, then the possibilities of middle ear baratrauma and damage of pressure on the patients can be prevented.]]> 2018/7/16 16:36:19 KONG Lei, XU Li-min, PAN Xiao-rong, et al. 876true ［Abstract］　Objective　To investigate the relevant factors of intraductal thrombosis after deep venous catheterization. Methods　Nineteen seriously ill patients with normal coagulation function in EICU of the people′s Hospital of Guangxi Zhuang Autonomous Region were chosen. CRP、D-Dimer, PLt, PT, APTT were test before Deep venipuncture and after 48 hours. The deep venous catheters were pulled out and the intraductal thromboses were tested by pathological examination after 48 hours of deep venous catheterization. All the test results and the relevant factors of intraductal thrombosis after deep venous catheters were inserted were statistically analyszed. Results　Nineteen cases intraductal thrombosis were found after deep venous catheters were inserted. In the test result of CRP, D-Dimer, PLT, PT, there were statistically significant difference before and after deep venous venipuncture (P< 0. 05). Conclusion　The test of CRP, D-Dimer, PLt, PT can be used for monitoring the occurrence of intraductal thrombosis in deep venipuncture.]]> 2018/7/16 16:36:19 DENG Da-zhi 875true ［Abstract］　Objective　To assess the predictive value of abnormal pregnancy outcome with higher, lower and mean uterine artery Doppler resistance indices and pulsatility indices by receiver operating characteristic curves (ROC). Methods　A cohort study included 80 patients of hypertensive disorder complicating pregnancies, all the patients were followed up to postnatal, and drawed ROC for the adverse pregnancy outcome of small-for-gestational-age (SGA), newborns premature birth, cesarean section with the gold standard by uterine artery blood flow parameters. The parameters of the test included the lower, mean, higher UtA-RI and lower, mean, higher UtA-PI, then calculated the area under ROC curve (AUC) and compared them. Results　The lower, mean, higher UtA-RI and lower, mean, higher UtA-PI could predict adverse outcome of pregnancy, such as premature birth, SGA (P<0. 05). There were no statistically significant differences in the receiver operating characteristic curves （P>0. 05）. The areas under the curve (AUC) for ROC curves in the prediction of cesarean section by the lower, mean and higher uterine artery Doppler PI and RI were shown no statistically significant differences（P>0. 05）. Conclusion　As well as uterine artery Doppler pulsatility indices, Performance of UtA-RI in predicting abnormal pregnancy outcome of premature birth and SGA was no different for the lower, higher or mean RI of the two UtAs in the patients of late hypertensive disorder complicating pregnancies.]]> 2018/7/16 16:36:19 PAN Hua，CHENG Gui-rong, WU Xi-jin，et al. 874true ［Abstract］　Objective　To explore the neuroprotective effect of hyperbaric oxygen（HBO） combined with anti-parkinson granule on Parkinson′s disease (PD) rats.Methods　PD rat model was established by 6-OHDA.Model rats were randomly divided into 2 groups: model group（A） and treatment group(hyperbaric oxygen combined with anti-parkinson granule)（B）. The changes of the activities of SOD and GSH-Px， the content of MDA were determined by using immunohistochemistry and the changes of expression of TH、GFAP、Bcl-2 and Bax protein were determined by using immunohistochemistry in PD rats.Results　 The changes of ethology appeared obviously.The activities of SOD and GSH-Px significantly increased ，and the content of MDA decreased in HBO group compared to model group (P<0.05).The activities of TH， Bcl-2 significantly increased ，and the expression of GFAP、Bax protein content decreased in treatment groups compared with the model group (P<0.01 or P<0.05).Conclusion　Hyperbaric oxygen combined with anti-parkinson granule can restrain the degree of oxidative stress, elevate its antioxidant abilities and significantly restrain apoptosis in substantia nigra. It is an effective neuroprotective and thempeutic measure for PD model rats.]]> 2018/7/16 16:37:43 PAN Xiao-rong，HU Yu-ying，FAN Qiu-ping，et al. 873true ［Abstract］　Objective　To explore the effects of endothelin in different period of diabetic nephropathy (DN) through investigation of the changes of endothelin.Methods　One hundred and thirty-six patients with type 2 diabetes mellitus (T2DM) were divided into 4 groups. 40 patients without microalbuminuria period in DN0 group, 36 patients with microalbuminuria period in DN1 group, 30 patients over proteinuria period in DN2 group, 30 patients impaired renal function period in DN3 group according to level of microalbuminuria and renal function, and compared with 30 patients pre-diabetes mellitus ( impaired glucose regulation,IGR)(IGR group) and 30 normal people (NGT group),and body mass index(BMI), fasting plasma glucose( FPG), fasting serum insulin( FINS), glycosylated hemoglobin(HbAlc), urine album in excretion ratio( UAER ), urine endothelin-1(UET-1), serum endothelin-1 (SET-1), creatinine clearance rate(Ccr),serum lipid levels and hemorheology were detected in the observed objects.Results　Compared with normal people,SET-1/UET-1,UAER, serum lipid levels,hemorheology were statistically significant (P<0.01 or P<0.05). SET-1 /UET-1 and hemorheology increased with development of DN in different period of DN and pre-diabtes mellitus.UEAR, SET-1,UET-1 were respectively significantly correlated with FPG, HbAlc, serum lipid levels and hemorheology.Conclusion　Endothelial dysfunction appears in each period of DN. Its severity was positively correlated with the SET-1/UET-1. SET-1/UET-1 can be used for predicting the degree of endothelial dysfunction and the progress of diabetic nephropathy in clinical diagnosis.]]> 2018/7/16 16:37:43 HUANG Yong-chan, LV Yi-pei, YANG Pi-jian,et al. 872true ［Abstract］　Objective　To investigate the feasibility and safety in patients of right ventricular outflow septal pacing (RVS)with active fixation lead in the right ventricular outflow septum compared with traditional right ventricular apex (RVA) pacing.Methods　Forty patients received fully automatic dual chamber pacing (DDD) pacing were randomly divided into two groups， RVS pacing group and RVA pacing group. The pacing parameters in each group were analyzed during the operation and 1 month after the operation. X-ray exposure time in right ventricle electrode implantation and complications were compared between two groups.Results　No significant difference was found in the instant and following-up pacing parameters at 1 month after the operation between two groups(P>0.05). The X-ray exposure time in right ventricle electrode implantation was （315±69）s in RVS pacing group and (213±43)s in RVA pacing group and no significant difference was found between two groups(P>0.05). No complication was found either during the operation or during the following-up.Conclusion　RVS pacing is as safe and efficient as RVA pacing.]]> 2018/7/16 16:37:43 HU Chang-xing, XU Guang-ma, LIU ling, et al. 871true ［Abstract］　Objective　To study the relationship between oxidative stress and mechanism of post-stroke depression in patients with a new single cerebral lesion.Methods　(1)The patients were divided into two groups, post-stroke depression(PSD) and non-PSD group. The level of plsama malonidialdehyde(MDA), the activity of superoxide dismutase(SOD), ascorbic acid(P-VitC), lipid peroxide(P-LPO), plasma nitric oxide(P-NO) were measured.(2)Based on Hamilton Depression Rating Scale, the patients were divided into these groups, mild depression, moderate depression, severe depression group and MDA, SOD level were compared among 3 groups.Results　(1)Compared with non-PSD group, the SOD, P-LPO, P-NO level were higher in PSD group, but the MDA P-VitC level was lower.(2)Compared with mild depression group, and moderate group depression group, the SOD level was higher (P<0.05) and MDA level was lower(P<0.05) in severe depression group.Conclusion　(1)Oxidative stress may be an important mechanism in post-stroke depression.(2)Severe depression group may have stronger oxidative stress.]]> 2018/7/16 16:37:43 ZHANG De-min, WANG Rong-gui, ZHOU Li-yuan, et al. 870true ［Abstract］　Objective　To explore the application method of calibration target-type interlvcking nail sighting device in long bone fracture fixation.Methods　 Adjustable calibration target-type interlocking nail sighting device and non-adjustable calibration target-typt interlocking nail sighting device were used in A group and B group respectively.Results　Aiming deviation in A group can be corrected by sighting device, and the aiming deviation in B group can′t be corrected by sighting device,and switched to manual correction.Conclusion　Adjustable calibration target-type interlocking nail sighting device has a good adaptability.]]> 2018/7/16 16:37:43 YANG Yi-jing, HONG Ding-gang, WANG Xiao-zhu,et al. 869true ［Abstract］　Objective　To investigate the mastering condition of cardiopulmonary resuscitation(CPR) knowledge among new students in a transport school in Guangxi.Methods　A self-designed questionnaire was used for survey of new students in a transport school in Guangxi, and the survey results were analyzed.Results　The results showed that only 22.3% of new students had awareness of CPR, and the ones who can do the correct implementation of CPR accounted for 0.4%, but 90% of students were willing to participate in CPR training.Conclusion　The knowledge level of CPR in vocational students is not high. These students can be used as the preferred object of CPR training.]]> 2018/7/16 16:37:43 QIN Ying 868true ［Abstract］　Objective　To investigate the pathogenesis, clinical manifestations, diagnosis and treatment methods of hemorrhagic cerebral infarction, to improve awareness of the disease.Methods　A retrospective analysis of clinical data in 16 cases of hemorrhagic cerebral infarction was performed. These patients had a clear history of hypertension, and their MRI examination within 4h before the onset of thrombolytic therapy revealed signs of cerebral infarction. CT showed signs of intracranial hemorrhage in 15 cases 3 days after thromblysis and in 1 case 6 days after thromblysis. Three of them had hematoma greater than 30 ml.Results　No deaths was found in 16 cases. Three cases undergoing minimally invasive hematoma aspiration survived in good condition 30 days after surgery. GCS score showed 9 to 11 scores in 11 cases, 12 to 15 scores in 5 cases.Conclusion　Hemorrhagic cerebral infarction mainly occurred in 3 days after cerebral infarction. Too high or too low blood pressure, bleeding tendency, improper application of thrombolytic agents are the risk factors. Defining disease process and timely imaging diagnosis are premise to reduce mortality. Monitoring coagulation during thrombolytic therapy, positire hemostasis after bleeding, using drugs of reducing intracranial pressure and necessary surgery is the key to improving the efficacy.]]> 2018/7/16 16:37:43 SUN Chao-yan，YANG Chun-hua 867true ［Abstract］　Objective　To study the clinical effect of glutamine on endotoxemia in patients with severe chronic hepatitis B.Methods　Sixty patients with severe chronic hepatitis B were randomly divided into control group(n=30) and treatment group(n=30). The patients were given regular treatment, including hepatic protectants and anti-virology therapy in control group. The patients were given extra glutamine 10 g Tid in addition to regular treatment in treatment group. The whole treatment duration lasted two weeks. Endotoxin (ET), liver function and the effective rate were compared between the two groups.Results　The effective rate of the treatment group was 83.3% and that of the control group was 50.0%, which were significant difference (P<0.01). The total tests of liver function, serum endotoxin level showed positive changes in both of two groups (P<0.05 or P<0.01).Conclusion　Adding glutamine to regular treatment is an effective adjuvant therapy for patients with severe chronic hepatitis B.]]> 2018/7/31 15:05:07 GAO Meng, YANG Jian-rong, SONG Huai-yu, et al. 866true ［Abstract］　Objective　To investigate the tuberculosis (TB) knowledge among the floating population in Guangzhou, in order to provide basis for health education of tuberculosis knowledge among floating population.Methods　A self-administrative questionnaire survey was conducted among the floating population diagnosed with TB in first visit in Guangzhou Chest Hospital from Oct. 1st to Dec. 30th, 2010.Results　Overall awareness rates on TB relevant knowledge among surveyed people were 59.75%. The majority of them knew the infectivity, transmission routes and curability of TB, while less of them knew the main symptoms and free policies on TB. The scores of TB relevant knowledge were lower in age group ≥40（Z=-2.016， P=0.044） and in group with low educational levels（Z=-2.604，P=0.009）. Logistic regression analysis identified that the age factor had significant influence on scores of knowledge（OR=0.306，P=0.027）.Conclusion　There was little knowledge about TB in Haizhu District, Guangzhou, and it is important to provide health education widely and effectively.]]> 2018/7/31 15:05:07 ZHANG Ke-xun, LI Pei-zhu, JIANG Kun-hong, et al. 865true ［Abstract］　Objective　To compare the effect of 8 to 13 years old amblyopia with the rule astigmatism and against-the-rule astigmatism in the perceptual learning.Methods　One hundred thirty-eight (274 eyes) 8 to 13 years old patients with rule astigmatism′s amblyopia and against-the-rule astigmatism′s amblyopia received perceptual learning.After 2 years perceptual learning,the effects between the rule astigmatism′s amblyopia and against-the-rule astigmatism′s amblyopia were analyzed. Results　In the 8 to 13 years old astigmatism′s amblyopia,the number of the serious amblyopia in patients with the rule astigmatism′s amblyapia was higher than that in patients with against-the-rule astigmatism′s amblyopia.The total effect in patients with against-the-rule astigmatism′s amblyopia(62.7%) was higher than that in patients with the rule astigmatism′s amblyopia(35.4%).Conclusion　Patients with against-the-rule astigmatism′s amblyopia has a higher total effect than patients with the rule astigmatism′s amblyopia in the perceptual learning.]]> 2018/7/31 15:05:07 KONG Min,LIN Quan,XIAO Xin,et al. 864true ［Abstract］　Objective　To establish the standard experimental protocol for the genetic testing of spinocerebellar ataxia(SCA) in order to avoid any mistake. Methods　Taking spinocerebellar ataxia 3(SCA3) as examlpe the genetypes of the patients were tested with PCR, sequencing, and the collection of the samples, the interpretation of the results and the protection of proprietary genetic information etc. were described in detail.Results　The abnormal amplifications of CAG repeats were present in SCA3 among 21 patients with SCA3 from 6 families.Conclusion　The standardization of experimental protocol of genetic testing can not only serve as basis of diagnose in some diseases, only when proprietary gene data of the subject is kept with strict secrecy,but estimate the dangerous degree of suffering from disease, and has important and positive significance for early precaution of the disease.]]> 2018/7/31 15:05:07 DING Ye, TAN Jian-qiang, HUA Rong, et al. 863true ［Abstract］　Objective　To evaluate the effects of 4-acetoxy-2-benzoxazolone（AcO-BOA）on proliferation of rat hepatic stellate cells (HSC-T6) in vitro. Methods　Rat HSC-T6 was incubated with different concentrations of AcO-BOA (0.008 mg/ml，0.04 mg/ml，0.2 mg/ml，1.0 mg/ml，5.0 mg/ml respectively) or without AcO-BOA for 32 hours, respectively. The proliferation rates of the HSC-T6 was determined by MTT assay. The morphological changes of the HSC-T6 were observed with inverted fluorenscence microscopy.Results　MTT assay indicated that the proliferation rates of HSC-T6 induced with different concentrations of AcO-BOA were significantly reduced compared with the control group incubated in vitro for 32 h ( all P<0.05), except the group whose concentration was 0.008 mg/ml (P>0.05). AcO-BOA significantly inhibited the proliferation of HSC-T6 in a dose-dependent manner. After acridine orange staining, inverted fluorenscence microscopy showed the morphological changes of HSC-T6, including depletion in numbers, cells shrinkage, karyorrhexis and karyopycnosis, and the changes were more significant as the concentration increased.Conclusion　The inhibitory effect on cell proliferation of AcO-BOA really exist.]]> 2018/7/31 15:05:07 FAN Hui, QI Ping, LIU Lin, et al. 862true ［Abstract］　Objective　To explore influence of duodenoscopic minimally invasive treatment on cardiorespiratory function of patients with pancreatobiliary diseases.Methods　In 45 patients with pancreatobiliary diseases undergoing duodenoscopic minimally invasive treatment,the changes of preoperative,intraoperative and post-operative systolic blood pressure(SBP), diastolic blood pressure (DBP), heart rate(HR),saturation of blood oxygen（SpO₂), were analyzed in order to evaluate the influence of duodenoscopic minimally invasive treatment on cardiorespiratory function of patients with pancreatobiliary diseases.Results　In 45 patients, 40 patients (88.89%) were successful operated in the first duodenoscopic treatment, 5 patients (11.11%) were successful operated in the second duodenoscopic treatment. Intraoperative HR was higher than preoperative and post-operative HR（P<0.05）, there are no significant difference between preoperative HR and post-operative HR（P>0.05）. Preoperative, intraoperative, post-operative SBP, DBP, SpO₂ did not have significant difference（P>0.05）. Conclusion　Duodenoscopic minimally invasive treatment is safe and effective without significant influence on cardiorespiratory function, and should become the first treatment method for patients with pancreatobiliary diseases.]]> 2018/7/31 15:05:07 WEI Liang-hong, CHEN Feng-kun,CHEN Hai-dong,et al. 861true ［Abstract］　Objective　To observe the regulation effects of Wenyangyishentang on lymphocyte subtypes.Methods　One hundred and two patients with kidney yang deficiency were radomly divided into two groups. Patients in two goups were treated with compound vitamin B tablets. The patients in observation group were treated with Wenyangyishentang on the basis of above treatment. The treatment course was two weeks,and the changes of lymphocyte subtypes were observed after treatment. Results　The increase of CD₄⁺ lymphocyte in observaion group was higher than that in the control group（P<0.05）. Conclusion　Wenyangyishentang can increase the CD₄⁺ lymphocyte which may be the point of regulating immune of enrich kidney.]]> 2018/7/31 15:05:07 KUANG Jin-bo,DENG Yu,SUN Jun,et al. 860true ［Abstract］　Objective　To quickly construct a directional cDNA library from human gastric carcinoma tissues.Methods　The total RNA was separated from mixed human gastric carcinoma tissues, then the first strand cDNA was synthesized with oligo(dT)primer containing Sfi I-digested sites before the double-strand cDNA was amplified through LD-PCR (long-distance PCR) by SMART technology. The double-strand cDNA was digested by Sfi I(IA ＆IB)restriction enzyme before cDNA size fractionation, the double-strand cDNA fractionated was ligated into λTripIEx2 vector and packaged in vitro.Results　The unamplified human gastric carcinoma tissues cDNA library consisted of 2.73×10⁶ independent clones with recombinant clones was 94%. The average size of the recombinants insert 1.2 kb.Conclusion　The quality of the constructed human gastric carcinoma tissues cDNA library is excellent and helpful to screen human gastric carcinoma specific antigen.]]> 2018/8/1 8:23:53 WU Kun，XIE Yu-bo, WANG Lian-zhen, et al. 859true ［Abstract］　Objective　To observe effect of atorvastatin on nuclear factor κB(NF-κB) activity in peripheral blood mononuclear cell,as well as serum inflammation factors including high sensitivity C reactive protein (hs-CRP)，tumor necrosis factor-a (TNF-a)，interleukin-6(IL-6), interleukin-1β(IL-1β) and insulin resistance in type 2 diabetes mellitus elderly patients with early nephropathy.Methods　There were 66 type 2 diabetics with early nephropathy in this study. Diabetics randomly divided into two group: type 2 diabetic routine treatment group (control group,n=31) and type 2 diabetic routine treatment combined with atorvastatin (treatment group,n=35). Level of NF-κB, inflammation factors and HOMA-IR were compared between pre-treatment and post-treatment in two groups.Results　After twelve weeks of treatment, level of NF-κB, inflammatory factors and HOMA-IR were significantly decreased in treatment group(P<0.05). Those were not decreased in control group (P>0.05).Conclusion　Atorvastatin might benefit patients with diabetes mellitus by improving inflammatory state,as well as insulin resistance.]]> 2018/8/1 8:23:53 LI Jian-ying，WANG Zhi-ying，CHEN Jian，et al. 858true ［Abstract］　Objective　To explore the level and significance of serum retinol binding protein 4 (RBP4) in the pregnant women with gestational diabetes mellitus.Methods　The levels of serum RBP4 in pregnant women with GDM (50 cases) and in normal pregnant women (50 cases) were tested individually by ELISA. Fasting plasma glucose （FPG）,glycosylated hemoglobin (HbA1c), fasting insulines (FINS), homeostasis modal assessment insulin resistance (HOMA-IR) and homeostasis modal assessment for beta cell function index (HOMA-β) were also tested.Results　(1)The level of serum RBP4 in pregnant women with GDM (18.48±4.60)ng/ml was significantly higher than that in normal women (13.26±2.35)ng/ml (P<0.01).(2)The levels of FPG, HbA1c and FINS in pregnant women with GDM［(5.40±0.57)mmol/L;(5.68±0.58)%;(9.32±1.30）mmol/L］ were significantly higher than that in normal women［(4.99±0.27)mmol/L;(4.75±0.51)%；(8.24±0.77)mmol/L］(P<0.01). (3)The HOMA-IR of pregnant women with GDM (2.24±0.43) was significantly higher than that in normal women (1.82±0.24)(P<0.01). There was not significant difference between the two groups of pregnant women on HOMA-β［women with GDM: (107.29±35.54) VS normal women: (112.02±19.35) (P>0.05)］. (4)The results of Pearson correlation analysis showed that the level of serum RBP4 in two groups of pregnant women were positively correlative with the level of HbA1c and FINS significantly (r=0.400,0.266， P<0.05), while were not obviously correlative with the level of HOMA-IR and the HOMA-β in advanced stage of pregnancy (r=0.072, 0.029, P>0.05).Conclusion　The level of serum RBP4 in pregnant women with GDM obviously increase. And the level of serum RBP4 may play a role in pregnant women with GDM,but there were no obvious correlation between the RBP4 and IR in these women;the abnormality of pancreatic islet beta cell function was not revealed yet in the GDM pregnant women.]]> 2018/8/1 8:23:53 REN Li-rong, YU Yan, HUANG Xiao-hong, et al. 857true ［Abstract］　Objective　To explore the expression of zinc-fingers and homeoboxes 2(ZHX2) in hepatocellular carcinoma(HCC) and their relationship with metastasis.Methods　RT-PCR was used for detecting the ZHX2 mRNA of 35 HCC tumor tissues and adjacent-tumor liver tissues and 8 cases of normal liver tissues.The results were analyzed by combination with the clinical and pathological data of the patients.Results　The positive rate of ZHX2 mRNA in tumor tissues was lower than those in adjacent-tumor liver tissues and normal liver tissues(P<0.05).The positive rate of ZHX2 mRNA in cases with preoperative metastsis was lower than that in cases of without metastsis(P<0.05).The expression of ZHX2 mRNA was correlated with the serum level of AFP(P<0.05),it was not correlated with age,sex,serum of HBsAg, tumor size,differentiation grade，liver cirrhosis.Conclusion　Metastasis of HCC and high expression of AFP were related with low expression of ZHX2.]]> 2018/8/1 8:23:53 CHEN Wei-lu, LV Zi-li 856true ［Abstract］　Objective　To investigate the effect of ulinastatin on the expression of intercellular adhesion molecule-1(ICAM-1) in canine myocardium during the CPB, evaluate whether ulinastatin could influence the expression of ICAM-1 and its protective effect on myocardium.Methods　Twenty dogs were randomly divided into two groups: ulinastatin group and control group.In ulinastatin group (n=10) dogs received ulinastatin 20 000 unit·kg^-1, half was added into the CPB priming fluid, and another half was given by intravenous injection before CPB running. In control group (n=10) dogs received CPB conventionally, the same volume of saline instead of ulinastatin. Right atrial appendage of the dog was obtained at 10 minutes before CPB running (point A) and at 60 minutes after CPB running (point B). The expression of ICAM-1 in cardiac myocytes was measured. And the establishment and running time of CPB,arotic cross -clamp time, aortic perfusion time and heart re-beat were recorded.Results　The expression of ICAM-1 in myocardial tissue after CPB in both groups increased and it was higher in control group than that in ulinastatin group (P<0.05).Conclusion　Myocardial ischemical reperfusion injury can cause the up-regulation of expression of ICAM-1 in myocardium, ulinastatin can restrain the expression of the ICAM-1,thus protecting the myocardial tissue by inhibiting the myocardial inflammation.]]> 2018/8/1 8:23:53 YANG Shi-xiong,LIU Qing-yuan, ZENG Jian-ye, et al. 855true ［Abstract］　Objective　To evaluate the therapeutic effects of auto-dermal transplantation,mesh plug filling and Bassinis for hernia repair of adult inguinal hernia.Methods　A prospective randomized clinical trial was carried out from January 2007 to January 2010，comparing the clinical effects among these three groups.Results　In auto-dermal transplantation group operation time，bed time，hospital stay were shorter and relapse rate was lower.Conclusion　Auto-dermal transplantation is a simple and effective hernia repair method for adult inguinal hernia,and reduces the cost of hospitalization obviously.]]> 2018/8/1 8:23:53 LIAO Wen-lun, QIU Fu-kui, ZHU Li-juan 854true ［Abstract］　Objective　To establish the normal reference values of serum folic acid and vitamin B₁₂ among the male Han people of the Fangchenggang area of Guangxi.Methods　Roche Cobas E601 electrochemical immunoassay was used to measure the serum folate and vitamin B₁₂ levels among the 2 021 male Han people aged 20～69.Results　Normal reference values of serum folic acid among the male Han people of the Fangchenggang area of Guangxi were: 8.56(4.97～14.71)ng/ml in 20～29 years, 9.25(5.24～15.42)ng/ml in 30～39 years, 9.66(5.35～16.38)ng/ml in 40～49 years, 9.31(5.49～16.60)ng/ml in 50～59 years, 9.44(4.58～14.86)ng/ml in 60～69 years. Normal reference values of vitamin B₁₂ among the male Han people of the Fangchenggang area of Guangxi were: 639.80(295.68～1 196.75)pg/ml in 20～29 years, 665.10(325.70～1 201.00)pg/ml in 30～39 years, 690.70(250.55～1 214.50)pg/ml in 40～49 years, 710.30(331.34～1 343.70)pg/ml in 50～59 years, 699.85(180.42～1 446.88)pg/ml in 60～69 years.Conclusion　It is suggested that every region should establish respective normal reference values of healthy person, especially the region which have different diet and lifestyle habits.]]> 2018/8/1 8:23:53 DENG Yan, QIN Xue, HUANG Shan, et al. 853true ［Abstract］　Objective　To explore the relationship between several common haplogroups and sporadic Parkinson′s disease(PD )in Chinese.Methods　Extraction of total genomic DNA from venous blood cells of 79 PD patients and 63 healthy controls was performed by hydroxybenzene-chloroform method respectively, the 4 single nucleotide polymorphisms (SNP) which is related to the several common haplogroups were genotyped by PCR-RFLP method.Results　2 individuals of haplogroup A, 4 individuals of haplogroup C, 5 individuals of haplogroup D, 2 individuals of haplogroup G were found in the PD patients,and 3 individuals of haplogroup A, 9 individuals of haplogroup C, 8 individuals of haplogroup D,only 1 individuals of haplogroup G were found in the healthy controls. So individuals classified as haplogroup G have the highest relative risk of PD,whereas individuals classified as haplogroup C have the lowest relative risk of PD, but there was no significant difference（P>0.05）. Conclusion　There was no advantage haplogroup among the fours in our study, while individuals classified as haplogroup C have the lowest relative risk of PD, additional large sample stratified studies of sex and age will be need to confirm the protective effect of the haplogroup.]]> 2018/8/1 8:23:53 GUO Cai-hua, YUAN Zhi-gang, LUO Shu-guang, et al. 852true ［Abstract］　Objective　To establish a method for isolation and culture of bone marrow mesenchymal stem cells (MSCs) from Bama minipigs in vitro and to identify characteristic of the cells.Methods　MSCs were isolated and cultured from the bone marrow of Bama minipigs by gradient centrifugation followed by adherent method.The morphology of MSCs was observed under phase contrast microscope.The expression of CD71 and CD34 of the third generation cells were analyzed by flow cytometry. Results　After 6 hours primary culture，MSCs adhered to plastic surface of the culture dish. About 7～9 days later, the cells proliferated in colonies.Primary MSCs reached 95% of confluence between 14 and 21 days approximately.The positive rates of CD71 and CD34 in MSCs at third generation were 95%, 0%, respectively. Conclusion　MSCs can be successfully isolated and cultivated by gradient centrifugation followed by adherent method.And higher purity MSCs can be picked up after culture expansion at passage 3.]]> 2018/8/1 9:43:06 LIN Ying-zhong, WANG Hong, LIU Fei, et al. 851true ［Abstract］　Objective　To utilize lateral fluid percussion traumatic brain injury combined bone fracture model to study the effect of the calcitonin gene-related peptide(CGRP) on the healing of fracture.Methods　Sprague-Dawley rats were randomly divided into three experimental groups: controls (A group,n=10), fracture only (B group, n=48),traumatic brain injury combined fracture (C group,n=48), the combined Sprague-Dawley rat model of traumatic brain injury and tibia fracture was established by fluid lateral percussion and opened fracture respectively. The CGRP value at 3^rd hour,1^st,3^rd,5^th,7^th,14^th day in the plasma of Sprague-Dawley rat after injury at the same time were continuously measured by radioimmune assay. The cell of callus was measured by immunohistochemistry. Bone fracture sample were analyzed by X-ray to study the healing of bone fracture,the size of callus and to meassure the diameter of the callus and biomechanical testing at the 21^st,28^th day.Results　After injury in all the groups,the concentrations of CGRP were increased,the peak was on the 7^rd day in C group,but on the 5^th day in B group,then its level in all groups decreased; The levels of serum CGRP of the C group was much higher than that of the B group, the difference was significant(P<0.05) and its last time was longer in C group; at 21 and 28 days after fracture,X-ray photography suggested that the size of callus in C group was significantly larger and denser than those in the B group.In addition the diameter and the stiffness of callus in C group were greater than those in B group, the difference were significant (P<0.05). CGRP was strongly expressed in osteoblasts and chonderocytes and fibroblast in group C.Conclusion　The time of fracture healing of rats with brain injury is shorter than that in rats with the bone fracture only, it could supposed that CGRP in serum could promote the healing of fracture]]> 2018/8/1 9:43:06 LI Bing, LUO Tong-qing, HU Zhao-hui 850true ［Abstract］　Objective　To observe acute toxicity of the water extract from old leaves of Guangxi kuding tea in mice.Methods　The water extract from old leaves of Guangxi kuding tea was administered orally to mice with the greatest concentration and the largest volume. The administration was given once daily for seven consecutive days and the toxicity in mice was recorded.Results　All experimented mice were survival and healthy. No significant change in body weight of mice and their toxicity were observed. The calculated maximum tolerant dose was 87.2 g/kg in mice, equivalent to that of 174.4 times in adults.Conclusion　The water extract from old leaves of Guangxi kuding tea has lower toxicity.]]> 2018/8/1 9:43:06 FU Cui-li, MENG Da-ping, RONG Yan-ping, et al. 849true ［Abstract］　Objective　To evaluate the CT diagnostic findings of inflammatory solitary pulmonary nodule to improve diagnostic ability.Methods　The CT imaging findings of 16 patients with inflammatory solitary pulmonary nodules confirmed by histology and clinical history were analyzed.Sixteen patients underwent high-resolution plain scan and dynamic contrast-enhanced CT.Results　The diameter of the largest nodule was 2.9 cm,the diameter of smallest nodule was 1 cm.The nodules were round and oval. Six nodules had well-defined margin and 10 nodules coarse margin.Four nodules had small lobular appearance.No nodule had satellite lesion and vascular convergence and calcification. The time density curve (TDC) of 15 nodules increased rapidly to the peak height and then declined quickly,the TDC of 1 nodule increased moderately and maintained relatively constant CT values.Conclusion　The combination of the morphological and functional imaging diagnosis can increase the rate of correct diagnosis of inflammatory solitary pulmonary nodules.]]> 2018/8/1 9:43:06 WEN Xue-xing, TENG Yin-ru, ZHAO Hua-lin,et al. 848true ［Abstract］　Objective　To investigate the expression of tumor suppressor gene p16 in verrucous gastritis and gastric cancer and the clinical significance. Methods　 Immunohistochemistry was used for testing the P16 protein of p16 gene in 56 cases of gastric cancer, and their adjacent tissues, 60 cases of verrucous gastritis and 60 cases of chronic superficial gastritis. Results　The degree of expression of P16 protein by order was gastric cancer→their adjacent tissues→verrucous gastritis→chronic superficial gastritis. Expression of P16 protein was lowest in gastric cancer (P<0.05); Expression of P16 protein between adjacent tissues and verrucous gastritis showed no significant difference (P>0.05), and expression of P16 protein in verrucous gastritis was greatly lower than that in chronic superficial gastritis (P<0.01).Conclusion　Tumor suppressor gene p16 may involve the process of development from verrucous gastritis to gastric cancer.]]> 2018/8/1 9:43:06 XIAO Chen, SU Dong-xing, PANG Zhi-gang, et al. 847true ［Abstract］　Objective　To observe the efficacy of treatment of intractable sudden deafness by injecting dexamethasone to drum chamber via eustachian tube.Methods　Dexamethasone 5 mg was injected to drum chamber via drum chamber under video laryngoscope in 36 cases(36 ears) for whom routine therapy is ineffective. The pure tone hearing thresholds of injected ears were tested at 1 days before and at 3 days after treatment respectively. The limiting mean of pure tone audiometry(PTA) in impaired fraquency was compared，decreasing more than 15 dB HL was defined as effective.Results　20 cases were effective, 16 casese were ineffective in 36 cases. The effective rate was 55.56%. PTA was (71.63±10.60)dB HL before treatment，PTA was (43.07±12.33)dB HL after treatment. There was a significant decrease(P<0.05).Conclusion　Injecting dexamethasone to drum chamber via drum chamber is a effective secondly therapeutic mean for sudden deafness patients for whom routine therapy is ineffective，also can be used for treating sudden deafness patients with contraindication to hormone therapy.]]> 2018/8/1 9:43:06 CHEN Shuang, HUANG Yue, YI Jing-cheng,et al. 846true ［Abstract］　Objective　To investigate the alteration of gene expression profiles between nasopharyngeal carcinoma (NPC) and non-neoplasm tissues then identified significant differentially expressed genes from NPC as novel markers.Methods　In our study, cDNA microarrays, which contain 14 112 points of full length human genes, in 20 NPC patients and in 15 nasopharyngeal chronic phlogistic tissues were used for revealing some new differentially expressed genes and molecular markers. Further, real time PCR was used to validate results of 2 groups in additional 50 poorly differentiated NPC tissues.Results　Nine significant differential expression genes（q<0.01）, with 2-fold or more alteration expression, were found differential expression in more than 17 (85%) specimens. Of them, 8 genes were over-expression (TAOK3，SLC16A2，PRB4，AMY2B，B3GALT4，MSMB，RPS27，CR2) were and under-expression (MXLIP) and 1 gene was up-expression. For further research, CR2 and MXLIP expression were detected by real time PCR in additional 50 NPC specimens. Compared with 3 nasopharyngeal chronic phlogistic tissues, CR2 was lower expression in 41 (82%) specimens and MXLIP was over expression in 42 (84%) specimens. This result was consistent with our microarray data.Conclusion　These two differentially expressed genes could be used as novel markers for poor differentiation NPC.]]> 2018/8/17 8:09:35 JIANG Zhen-ling, CHEN Jia-xin, LIN Hui, et al. 845true ［Abstract］　Objective　To observe the effect of cardiac rehabilitation exercise training on prognosis of coronary heart disease.Methods　One hundred and two inpatients in accord with diagnostic criteria for coronary heart disease developed by WHO in 1979 excluding patients with class Ⅲ or more, and patients with joint or lower extremity disease, successively by odd and even numbers on admission were assigned to rehabilitation group and control group, 51 patients in each group, blood pressure, lipids,electrocardiogram, echocardiography, carotid ultrasound, ECG treadmill stress test and 6-minute walking test and other tests were perforned in two groups. After evaluation of cardiac function, rehabilitation group received supervised rehabilitation exercise training in accorelance with individualized rehabilitation exercise training prescription, control group did not receive movement guidance. All items were reviewed after 24 weeks.Results　During the observation of the two groups no one quit because of an accident. After exercise traning,angina pectoris significartly reduced in patients with angina pectoris in rehabilitation group, with 32 patients without angina (P< 0.01). ECG were improved, with patients with ST-T to normal (P< 0.01). Blood pressure,ventricular septal,left ventricular posterior wall, TC,LDL,carotid intima-media thickness and plaque improved (P< 0.01), 6 min walking distance and metabolic equivalent increased (P< 0.01).Except LDL,there were statistical difference in TC,EF,6 min walking distance and carotid plaque between two groups.Conclusion　A guided and individualized rehabilitation exercise training is helpful in improving heart function, the prognosis of coronary heart disease and exercise tolerance for patients with coronary heart disease.]]> 2018/8/17 8:09:35 PANG Ming, SHI Wen,GUANG Hao-zhen, et al. 844true ［Abstract］　Objective　To evaluate the expression and significance of EMMPRIN in tissues of non-small cell lung cancer (NSCLC).Methods　Reverse transcription polymerase chain reaction (RT-PCR) was used for measuring the expression level of EMMPRIN in tissues from 80 patients with NSCIC and in tumor adjacent tissues from 40 patients with NSCLC.Then the sequence of PCR products of EMMPRIN gene was analysed.Results　The positive expression rate of EMMPRIN in tissues of non-small cell lung cancer was significantly higher than that in tumor adjacent tissues (P<0.05). EMMPRIN expression was positively correlated with lymph node metastasis (P<0.05).Conclusion　EMMPRIN may take part in the progress of NSCLC, and may be used as good indicator for judging metastasis, clinical stage, and prognosis of lung cancer.]]> 2018/8/17 8:09:35 DAI Lei，XIAN Lei，CHEN Ming-wu 843true ［Abstract］　Objective　To evaluate the safety and effectiveness of semiconductor laser peripheral iridoplasty for treatment of primary angle-closure glaucoma complicated with acute attack. Methods　Twenty-eight 28 eyes of 25 cases with primary angle-closure glaucoma complicated with acute attack diagnosed in our hospital were treated by semiconductor laser peripheral iridoplasty. Intraocular pressure （IOP） at 15 minutes, 30 minutes,60 minutes,90 minutes and 24 hours after laser were detected.Results　The mean preoperative IOP of 28 eyes was (58.4±1.9)mmHg, at 15 minutes after laser the mean IOP was (49.3±2.1)mmHg, with decrease of 15.54%; at 30 minutes after laser the mean IOP was (39.3±3.3)mmHg, with decrease of 32.76%, at 60 minutes after laser the mean IOP was (29.4±3.3)mmHg, with decrease of 49.57%, at 90 minutes after laser the mean IOP was 23.03 mmHg, with decrease of 60.61%. There were significant statistical difference between IOP before laser and IOP at every points after treatment （P<0.05）. At 24 hours after laser, IOP of 20 eyes were ≤21mmHg, and IOP of 8 eyes≥21mmHg, with significant decrease compared with that before laser. There was no case whose postoperative IOP was higher than that before laser. There were no serious complications in all cases.Conclusion　Semiconductor laser peripheral iridoplasty for the treatment of primary angle-closure glaucoma complicated with acute attack is a safe and effective method.]]> 2018/8/17 8:09:35 ZHONG Shan, LI Li 842true ［Abstract］　Objective　To explore the application value of transvaginal hydrolaparoscopy combined with uterine manipulator under ultrasound in infertility. Methods　Sixty-eight patients with infertility which were suitable for uterine manipulator combined with transvaginal hydrolaparoscopy were examined under ultrasound， and the pelvic cavity situation was observed. Results　The puncture needling instrument was seen in pelvic cavity in 68 patients in first time of puncturing. In 66 patients laparoscope was put in uterus rectum lacuna along the puncture path successfully. The puncture success ratio was 97.05% (66/88). Under ultrasound, in 26 patients the pelvic adhesion in liquid pool was shown, accounting for 33.23% (26/68). Also,under laparoscopy, pelvic adhesion was shown in 31 patients, accounting for 45.58% (31/68). Compared these two examinations, coincidence rate was 83.87% (26/31). Twenty-two patients had to be changed to standard laparoscopy for severe pelvic adhesion or fimbria tube uterine atresia, accounting for 32.4%(22/68). There was no complication, such as rectum damage, bleeding after operation in puncturing place and pelvic cavity infection. Conclusion　The success ratio of puncturing through vagina under ultrasound is high. It can avoid accidental pelvic organs injuries. The result of pelvic adhesion shown by ultrasound resembles that of laparoscopy. It is helpful for diagnosis and treatment of infertility.]]> 2018/8/17 8:09:35 WU Xi-jin, HUANG Yu-kui,GAN Bai-liu,et al. 841true ［Abstract］　Objective　To explore the reference values and influential factors of brachial-ankle pulse wave velocity（baPWV）for She population of Fujian province.Methods　In the year 2009, 5 523 participants of She nationality living in Ningde city of Fujian province were multistage-stratified randomly selected, and a total of 907 identified healthy subjects were ultimately recruited in this study. Subjects were all underwent physical measurements, blood biochemical analysis and baPWV measurement.Results　For healthy population, the mean value of baPWV was higher for male (P=0.022). Multiple regression analysis revealed that both age and systolic blood pressure were positively associated with baPWV for male and female. In addition, we also found diastolic blood pressure was slightly and positively associated with baPWV, but heart rate was inversely related to baPWV in females. The 95% of upper limits of baPWV for subjects aged 20～29 yr, 30～39 yr, 40～49 yr, 50～70 yr were 1466/1087 cm/s, 1 602/1 221 cm/s, 1 606/1 255 cm/s, 1 964/1 738 cm/s, respectively (males/females).Conclusion　Age and systolic blood pressure were the mainly influential factors for baPWV in She nationality of Fujian province，the age- and sex-stratified reference values of baPWV are very useful for screening.]]> 2018/8/17 8:10:44 LAI Xiao-lan, CHEN Gang, ZHU Shao-heng, et al. 840true ［Abstract］　Objective　To investigate the effect of application of the ProSeal laryngeal mask during general anesthesia laparoscopic surgery.Methods　Eighty ASAⅠorⅡpatients undergoing elective surgery were randomly divided into two groups group received tracheal intubation. After induction of general anesthesia, laryngeal mask group received ProSeal laryngeal mask (Ⅰgroup),and tracheal intubation group received tracheal intubation (Ⅱgroup). Before induction (T₀, baseline), immediately before intubation (mask) (T₁), at 1 min after intubation (T₂), at 5 min after intubation (T₃), at 5 min after pneumoperitoneum (T₄), at 10 min after pneumoperitoneum (T₅), at 1 min after pneumoperitoneum release (T₆), after extubation (mask) (T₇) blood pressure, heart rate; related respiratory parameters: Inhaled and exhaled tidal volume (Vti and Vte), inhalation and exhalation of minute ventilation (MVi and MVe), peak airway pressure (Ppeak) and plateau pressure (Pplat), lung compliance (CL) and airway resistance (Raw)were recorded;The side effects were observed. Results　The difference in T₀ MAP and HR between the two groups was not significant (P>0.05); Compared with I group, HR was faster and MBP was ligher in Ⅱ group after anesthesia (P<0.05)； compared with P_ETCO₂ before pneumoperitoneum, P_ETCO₂ after pneumoperitoneum incrensed significantly. There was significant difference in post operative throat discomfort and sore between two groups(P<0.05). Conclusion　The ProSeal laryngeal mask is safe and effective for laparoscopic cholecystectomy.]]> 2018/8/17 8:10:44 MENG Shu-qin，WANG Xiao-nan，DING Ling-ling，et al. 839true ［Abstract］　Objective　To explore the myopic diopter dynamics of the myopic amblyopia during the treatment of perceptual learning.Methods　Refractive diopter of the 54 children with myopic amblyopia (98 eyes) were prospectively collected，which was selected from the amblyopia clinic between 2006 and 2009, and the myopic diopter dynamics before and after perceptual learning were analyzed according to the degree of amblyopia, myopia and the curative effect.Results　(1) The spherical and spherical equivalent (SE) of children with myopia amblyopia after visual perception treatment was higher than that before treatment (P<0.01); the annual average change of spherical and SE were (0.52±0.79)D/year and (0.54± 0.81)D/year; the annual average growth of SE less than 1.00 D accounted for 76.53%, in contrast, average annual growth more than 1.00 D accounted for 23.47%. (2) The dynamic changes of spherical or SE of light, moderate and severe amblyopia have the significant difference (P<0.01), but the dynamic changes of cylinder was no significant difference between them. (3)The dynamic changes of spherical, cylinder and SE between the mild, moderate and severe myopia amblyopes was no significant difference (P>0.05). (4) the spherical and SE showed decreased trend from ineffective to cure, however, the dynamic changes of refractive diopter between different curative effects of amblyopia showed no significant difference (P>0.05).Conclusion　The myopia diopter of the myopic amblyopia increased after the visual perception treatment, which may be contributed to the growth of spherical diopter, refractive change of severe amblyopia is higher than those of mild or moderate degree of amblyopia, the myopic degree and efficacy have no effect on myopization.]]> 2018/8/17 8:10:44 XIAO Xin, LIU Wei-min, WANG Ying, et al. 838true ［Abstract］　Objective　To investigate the effect of glucocorticoid(GC) therapy on the sera levels of interleukin-6(IL-6) and B cell activating factor (BAFF) and the clinical efficacy in new onset patients with myasthenia gravis(MG).Methods　The sera levels of IL-6 and BAFF were measured in 26 patients with MG before and after-treatment and in 30 normal controls (NC) with ELISA. The effects of GC on patients with MG were assessed by XU Xian-hao score system.Results　The levels of IL-6 and BAFF of patients with MG were significantly higher than those of NC group（P<0.05）. The sera levels of IL-6 and BAFF were dramatically decreased after 2 months of treatment with GC. There were significant positive correlation between variation of IL-6 concentrations and the relative scores of MG before and after-treatment. Conclusion　The level of IL-6 were significantly positively correlated with the severity of MG, GC can effectively regulate the function of cellular immunity by inhibiting the production of IL-6 and BAFF.]]> 2018/8/17 8:10:44 WANG Min-li, LI Xiao-feng,ZHANG Li-xiang,et al. 837true ［Abstract］　Objective　To analyze the needs of breast reconstructions after mastectomy in patients with breast cancer.Methods　The data of five hunderd patients with breast cancer were collected and the investigation was done about needs of breast reconstructions after mastectomy in patients with breast cancer. Different ages, education,clinical pathematology （sizes, lymph nodes,status of hormone receptor）were compared with their needs for reconstruction.Results　More patients were satisfactied with breast prosthesis.There was a higher proportion in the needs of breast reconstructions in the patients under 45 ages.In addition, pathematology and complications influenced the needs of breast reconstructions.Conclusion　It is necessary to institute suitable remedial methods for patients after mastectomy according to different needs.]]> 2018/8/17 8:10:44 LI Qing-guo, XU Gang, WANG Jin-xing,et al. 836true ［Abstract］　Objective　To investigate and develop a Bama minipig model of acute myocardial infarction (AMI) by coronary occlusion with balloon inflations. Methods　After anesthesia, eight minipigs underwent coronary occlusion with balloon inflations which was positioned in the left anterior descending (LAD) coronary artery. The artery was blocked for 30 seconds for 3～4 times with an interval of 5～10 minutes every time, as a way of ischemic preconditioning. The balloon was inflated at 3～5 atm to occlude the LAD for 40 minutes. Results　Six of eight minipigs were survival after setting up the AMI model. The success rate was 75%. The coefficient of variation of myocardial infarction square in the model was 11.7%（33.2±3.9）. The dynamic ECG changes showed ST segment elevation after model setup. There was significant increasing in CK, CK-MB, cTnI, Myo and LDH after occlusion than before. There were larger in left ventricular end diastolic diameter (LVEDD), left ventricular end diastolic volume (LVEDV) and left ventricular ejection fraction (LVEF) was less after establishing AMI model than before. In some of minipigs, there was aneurysm after procedure. TTC staining and HE staining confirmed AMI in all minipigs. Conclusion　Bama minipig model of AMI can be established successfully by coronary occlusion with balloon angioplasty. This model can keep the coronary artery open, and make stem cell implantation become possible in the further study.]]> 2018/8/17 8:12:31 WANG Hong, LIN Yin-zhong, WU Xu-bin, et al. 835true ［Abstract］　Objective　To study the relationship of the serum adiponectin(APN) and C-reactive protein(CRP) with macroangiopathy in type 2 diabetic patients. Methods　Sixty-three type 2 diabetes patients were divided into two groups depending on the carotid intima-media thickness (CIMT) value (CIMT≥1 mm as group A, CIMT<1 mm as group B).The normal controls was enrolled in this study as control group (group C). The height, weight, blood glucose, blood fat, fasting insulin, serum APN, CRP and CIMT in these patients were determined. The body mass index (BMI) and insulin resistance index(IRI) in these patients were calculated, then above indexes were analyzed statisticaly. Results　The serum APN of group A and B were lower than that group C(P<0.01) and the serum APN of group A was lower than that of group B(P<0.01). The serum CRP of group A and B were higher than that of group C (P<0.01), and the serum CRP of Group A were higher than that of group B (P<0.05). CIMT was negatively correlated with APN and HDL， while positively correlated with HOMA-IR, CRP. FBG, HDL, CRP and APN were entered in the regression equation. Conclusion　The reducing of the level of serum adiponectin, the elevating of CRP are major risk factors of carotid artery intimal thickening in type 2 diabetes patients and involved in occurrence and development of macroangiopathy in type 2 diabetes patients.]]> 2018/8/17 8:12:31 SU Ke, LONG Yan, XUN Jing-qiong, et al. 834true ［Abstract］　Objective　To detect the change of T-bet expression and CD19/CD23 on peripheral blood in patients with allergic rhinitis (AR) after specific immunotherapy (SIT).Methods　Blood samples were respectively taken from 15 healthy individuals and 60 AR patients who had been accepted SIT in vivo for at least one year. The peripheral blood monouclear cells (PBMC) from two groups was subjected to analysis of T-bet mRNA expression by semiquaitative reverse transcriptase-polymerase chain reaction (RT-PCR).The level of CD19/CD23 was detected by flow cytometry (FCM) before and after SIT.Results　The ratio of T-bet to β-actin in 24 AR patients before and after SIT respectively was (0.44±0.14) and (0.49±0.09), between which the difference was statistically significant (t=2.324,P<0.05).In control group, the ratio was (0.59±0.09), which was higher than that in the two above AR group (P<0.01). CD23 (%) in control group and AR group was (5.99±2.44) and (13.53±5.41) (P<0.05). Between 42 AR patients without asthma and other 18 AR patients with asthmatic symptoms, the level of T-bet and CD23 was (0.49±0.12) and (0.33±0.09), (12.32±5.71) and (16.34±3.32), and t value correspondingly and respectively was 5.411 and 3.407, as well as P value correspondingly and respectively was 0.000 and 0.001. T-bet had a negative relation with CD23 (r=-0.260, P=0.045). It indicated significant reduction of CD23 after SIT (t=2.334, P<0.05). T-bet mRNA expression was significantly up-regulated after SIT (χ²=-2.324, P<0.05).Conclusion　The download-regulated level of CD23 and up-regulated T-bet expression in peripheral blood of AR patients can be observed after SIT. It develops a new view on SIT of AR that Th1 predominance at the level of transcription factor corrects Th1/Th2 imbalance in this way of mediation of up-regulation of Th1-associated transcription factor.]]> 2018/8/17 8:12:31 QU Shen-hong, LI Tian-ying, OU Zhi-ying, et al. 833true ［Abstract］　Objective　To investigate the relationship between serum levels of UA and the activity of disease in multiple sclerosis(MS) patients.Methods　Fifty-eight MS patients were divided into two groups: intrathecal methylprednisolone group and methylprednisolone impact group.The serum levels of UA in relapse was compared with those in remission after intrathecal injection of methylprednisolone in MS patients，and with those in non- inflammatory neurological diseases(control group). The degree of neurologic impairment were compared before and after methylprednisolone treatment. Results　The serum levels of UA in replase of MS were significantly lower than those in remission and control group. The clinical symptom and the neurologic impairment got better with ascending of the serum levels of UA in MS patients.Conclusion　Low serum levels of UA are closely related to the attack of MS. The serum levels of UA are helpful in judging the activity of MS and the theraputic effect of methylprednisolone treatment. Rasing the level of the serum uric acid may be an anticipant therapy.]]> 2018/8/17 8:12:31 DONG Yan-ling, LI lü-li, LI Yao-xuan,et al. 832true ［Abstract］　Objective　To explore the myopic diopter dynamic change of children with myopic amblyopia before and after the treatment of traditional comprehensive therapy.Methods　Refractive diopter of the 45 children (82 eyes) with myopic amblyopia were prospectivly collected，which was selected from the amblyopia clinic of Guangxi visual science and optometry center between 2007 and 2009, and the myopic diopter dynamics before and after traditional comprehensive therapy were analyzed according to the degree of amblyopia, myopia and the curative effect.Results　(1)The spherical and spherical equivalent (SE) of children with myopic amblyopia after traditional comprehensive treatment was higher than that before treatment (P<0.05); the annual average change of spherical and SE were (0.49±1.09)D/year and (0.54±1.09)D/year; the annual average growth of SE less than 1.00D accounted for 91.5%. (2)The dynamic changes of spherical or SE of light between moderate and severe amblyopia had the significant difference (P<0.05). (3)The dynamic changes of spherical, cylinder and SE between the mild, moderate and severe myopic amblyopia was no significant difference (P>0.05). (4)The dynamic changes of refractive diopter between different curative effects of amblyopia showed no significant difference (P>0.05).Conclusion　The traditional comprehensive therapy was less effect on the myopia diopter dynamics of the myopic amblyopia, refractive change of severe amblyopia is lower than those of mild or moderate degree of amblyopia.]]> 2018/8/17 8:12:31 WANG Ying, XIAO Xin, LIN Quan, et al. 831true ［Abstract］　Objective　To investigate the effect of sputum suction and related complications of lung inflation and clapping back in infant patients undergoing mechanical ventilation after open heart surgery.Methods　Forty mechanical ventilation infant patients after the intracardiac operations were selected. They must be meet the conditions: the mechanical ventilation time ≥72 h and age ≤3 y.And then they were randomly divided into group A and B.Group A received the lung inflation and clapping back for sputum suction. Group B received sputum suction normally. The state of the oxygen saturation, heart rate, and blood pressure, and the occurring rate of the ventilator associated pneumonia（VAP）in two groups were observed to study how to cure the patients.Results　There is no statistical significant differences in the diastolic pressure during the sputum suction between the two groups. Compared with Group B,in Group A, sputum suction was more thoroughgoing ;the occurring rate of the VAP was obviously lower. Compared with Group A, in Group B, inerease of heart rate, rising of systolic pressure and oxygen saturation drop were more commom. Conclusion　When give the mechanical ventilation infant patients sputum suction normally, it will bring on some disadvantage influences, such as tachycardia, rising of systolic pressure, no thoroughgoing sputum suction, rising of occurring rate of the VAP. So, For the mechanical ventilation infant patients, the best cure is to use the lung inflation and clapping back normally for sputum suction.]]> 2018/8/17 8:12:31 HE Jing, YANG Hong-ye, WEI Jing, et al. 830true ［Abstract］　Objective　To investigate the correlation between single nucleotide polymorphisms in the 3′ untranslated regions of HLA-G gene and asthma occurrence of children.Methods　Totally 84 children cases with asthma and 116 controls in Guangxi region were included into the study. The HLA-G gene single nucleotide poly-morphism (SNP) genotyping, rs1704 and rs1063320, was performed by using MassARRAY system (Sequenom) by means of matrix assisted laser desorption ionisation-time of flight mass spectrometry method (MALDI-TOF). And multiple regression analysis was performed to explore the asthma correlation.Results　There was significant difference in the distribution of the genotypes of rs1063320 between cases and controls (P<0.05). The individuals with rs1704/rs1063320 14-bp deletion/G haplotype had decreased risk of asthma (odds ratio ［OR］=0.344, 95% confidence interval ［95%CI］=0.149～0.799,P<0.05), and the association with asthma of rs1704/rs1063320 14-bp deletion/G combined HLA-DRB1^*15XX haplotype was more apparent with OR value of 0.198， 95%CI of 0.084～0.471(P<0.001), and with lower serum value of IgE in asthma cases (P<0.05).Conclusion　HLA-G 3′ UTR SNPs has correlations with etiology of asthma. Having the haplotype of rs1704/rs1063320 14-bp deletion/G are protective from children asthma.]]> 2018/6/28 8:50:28 ZHEN Hong, XIE Qing-ling, TANG Xiao-yan, et al. 829true ［Abstract］　Objective　To investigate the relationship between obese and lumbar intervertebral disc degeneration. Methods　A total of 150 persons were selected according to the same standard, 60 of them were lumbar intervertebral disc protrusion (LIDP) patients, others were with low back pain and/or legs pain but without lumbar disc protrusion, another 30 persons were in good health. Lumbar intervertebral disc degeneration (IDD) of them were tested by MRI, and the relationship between IDD and body mass index were analyzed.Results　It was showed that there was statistical difference in IDD between significant obese persons, overweight persons and persons in normal weight (P<0.05).Conclusion　There is an association between body index and lumbar intervertebral disc degeneration, and obese may be one of the risk factors of lumbar intervertebral disc degeneration.]]> 2018/6/28 8:50:28 XU Jian-wen, ZHONG Yuan-ming,YIN Li-jun,et al. 828true ［Abstract］　Objective　To observe the effect of silver needle acupuncture in the treatment of athletes with chronic soft tissue pain of low back,buttocks and lower limb.Methods　Thirty athletes patients with chronic soft tissue pain of low back,buttocks and lower limb were measured by temperature recorder of infrared thermography and visual analog scale(VAS) on the pretreatment,on the moment of treatment and on the 60 days after treatment.Results　The changes of VAS index were significantly improved on the 60 days after treatment (P<0.01) and the volume of temperature in patient s′tissue were increased significantly on the moment and on the 60 days after treatment(P<0.01).Conclusion　Silver needle acupuncture treatment has a reliable long term curative effect on athletes patients with chronic soft tissue lesion of low back,buttocks and lower limb.]]> 2018/6/28 8:50:28 JIANG Chong-bo,HOU Jing-shan,MA Xiu-jia, et al. 827true ［Abstract］　Objective　To investigate the effects of extraction orthodontic treatment on the upper airway dimensions in adolescent patients with classⅠmalocclusions.Methods　Nineteen adolescent patients with classⅠcrowding or bimaxillary protrusion malocclusions who were orthodontically treated by straight wire appliance and with 4 premolar extraction were studied.The period of the correction was 22 to 27 months. Cephalometric analysis of each patient were performed before and after treatment on the sagittal upper airway dimensions.The measurements were tested by statistical analysis.Results　After extraction treatment, the chang of most measurements of upper airway showed no significant difference (P>0.05)except the measurements of SPP-SPPW (P<0.01)in the patients of classⅠmalocclusions after the extraction treatment.Conclusion　The effects of extraction orthodontic treatment on the upper airway dimensions in adolescent patients with classⅠmalocclusions was limited.]]> 2018/6/28 8:50:28 FANG Zhi-xin, ZHOU Yan, CHEN Shi-wen, et al. 826true ［Abstract］　Objective　To observe the safety and efficacy of all total enflurane inhaled anesthesia in pediatric patients.Methods　Ninety pediatric patients operated under general anesthesia were randomly divided into three groups, age from 1 to 7 yr, 30 patients in each group. Group Ⅰ was induced and maintained by ketamine, midazolam and atracurium. Group Ⅱ was induced and maintained by ketamine, propofol atracurium, while the group Ⅲ was induced with enflurane and succinylcholine, then maintained with enflurane. The change of MAP, HR, SpO₂, P_ETCO₂ was observed at the time before anesthesia(T₀), 1 min after endotracheal intubation(T₁), beginning of operation(T₂), 30 min after operation(T₃), end of the operation(T₄) and 10 min after endotracheal extubation(T₅). The artery blood gas values at 1 min after endotracheal intubation, at the end of operation and 10 min after endotracheal extubation were checked. The waking time from end of operation, the time of endotracheal extubation and the incidence of nausea, vomiting and crying were recorded.Results　HR and MAP at T₁, T₂ were higher than those of other time points in each group and between each group(P<0.05),except the comparison between T₁ and T₂). The value of SpO₂ was no significant difference (P>0.05). P_ETCO₂ of group Ⅲ at T₄ was higher than other points in this group and other groups (P<0.05). The value of pH and PaO₂ are no significant difference at difference time point in every group and the same point between each group(P>0.05). The value of PaCO₂ at T₄ was higher greatly than that of at T₁ and T₅ in group Ⅲ and higher significantly than that of other groups at T₁ and T₅(P<0.05). The time of group Ⅲ from end of operation to endotracheal extubation and waking was significant shorter than that of other two groups(P<0.05). The time of group Ⅱ from end of operation to endotracheal extubation and waking time was significantly shorter than that of group Ⅰ(P<0.05). The anesthesia expenses in group Ⅲ was lower than that of the other groups, while incidence of crying was significant higher than other groups(P<0.05). The anesthesia expenses and the incidence of nausea, vomiting and crying was no significant difference between group Ⅰ and group Ⅱ（P>0.05）.Conclusion　A total enflurane inhaled anesthesia in pediatric patients is safety and reliable in non-cardiovascular, non-neurosurgery and non-liver operations. It has the advantages of rapid recovery, early extubation, less complications and lower expenses.]]> 2018/6/28 8:50:28 ZENG Jin, PENG Jun-hua, WANG Xue-fei, et al. 825true ［Abstract］　Objective　To evaluate the block effect of brachial plexus nerve block located by different transverse process on sensory and motor nerve branches.Methods　Two hundred adult patients,ASA Ⅰ～Ⅱ,received a different brachial plexus block by C₅,C₆, C₇ processus transverses approach according to different operative site.Sensory block grading and motor nerve branches block grading were evaluated, then complications were recorded.Results　Axillary nerve and C₄ nerve received a excellent block effect by C₅ processus transverses approach than C₆, C₇（P<0.05）; ulnar nerve block effect were significantly improved by C₇ processus transverses approach than C₆（P<0.05）.Conclusion　Brachial plexus block by different transverse process approach has different block effect on sensory and motor nerve branches.]]> 2018/6/28 8:50:28 ZHU Wei-lin,HUANG Zhong-hua,LI Feng,et al. 824true ［Abstract］　Objective　To assess the clinical efficacy of a heavy silicon oil as temporary internal tamponade in the treatment of retinal detachment without holes.Methods　Vitrectomy combined with intravitreal heavy silicone oil tamponade (Oxane HD)was performed in 7 eyes of 7 patients with diagnosed retinal detachment without holes.Results　The recurrence of retinal detachment was not be found in all 7 cases followed up for 89～248 days. Two cases showed improvement in visual acuity, 2 cases remained visual acuity and 3 cases showed visual acuity decreased after the surgery. After surgery intraocular pressure was abnormal in 5 cases, included 2 case lower than 10 mmHg and 3 cases higher than 21 mmHg. Tamponade was found in the anterior chamber in 2 case. No complicated cataract, silicon oil emulsification or excessive postoperative inflammation in anterior chamber were found in the follow-up period.Conclusion　Treating the retinal detachment without holes with vitrectomy combined with intravitreal heavy silicone oil tamponade could secure a satisfactory rate of retinal reattachment, whereas the incidence of complications is low and the comfortability of patients is improved.]]> 2018/6/28 8:50:28 LAN Qian-qian, LI Min,ZHAO Xin,et al. 823true ［Abstract］　Objective　To investigate the temperature of irrigating fluid on life signs of elderly patients undergoing the minimally invasive percutaneous nephrolithotomy lithotomy (mPCN).Methods　Ninety elderly patients undergoing mPCN were randomly divided into three groups by different temperature of fluid, each group 30 cases.The temperature of irrigating fluid group A were (20～24)℃, group B (28～30)℃, group C (36～37)℃. Body temperature (T), mean arterial pressure (MAP), heart rate (HR) and the incidence of shivering were observed when using different temperature fluid on patients.Results　In group C,T, MAP and HR showed no significance between before and after mPCN (P>0.05); the incidence of shivering in group C was lower than in group A or group B (P<0.01).Conclusion　Using irrigating fluid ranging from 36 to 37 degree ℃ in mPCN can reduce the changes of body temperature, blood pressure, heart rate and the incidence of shivering.]]> 2018/6/28 8:50:28 GU Chao-qiong,YANG Hong-yan,LI Xiao-yan,et al. 822true ［Abstract］　Objective　To investigate the expressions of aquaporin-9(AQP9) and its role in cultured rat astrocytes after exposure to injury.Methods　Rat cerebral cortical astrocytes from 2 days newborn Wistar rats were undergone the pure culture. The cells were divided into control group and injury group randomly, in which group was examined at interval times of 1, 3, 5 and 7 d, respectively, and its model of injury was established by scratching the cultured astrocytes. The reactivity and rules of AQP9 mRNA expression of astrocyte to injury were studied by morphological changes, immunocytochemistry, in situ hybridization and imaging analysis. Results　After scratching, the astrocytes showed topical features of reactive gliosis, with the hypertrophic cell body, thickened and lengthened processes, and enhanced glial fibrillary acidic protein(GFAP) staining was markedly increased. In situ hybridization analysis confirmed that the expression of AQP9 mRNA was remarkably increased. These changes occurred 1 d after scratching and reached the peak 5 to 7 d after injuring. These changes in the injury group were much more than those in the control group（P<0.05）.Conclusion　A model of reactive astrogliosis was successfully established in vitro which showed an active reaction to injury，and induced expression of AQP9 mRNA increased. The results suggest that AQP9 participated in the brain edema of injury formation.]]> 2018/6/28 8:51:28 LI Yan-hua, LI Lü-li, LUO Yong-jian, et al. 821true ［Abstract］　Objective　To observe the effect of excision of pterygium combined with autologous transplantation of limbal stem cells of different parts in treating primary pterygium.Methods　Fifty-one patients (62 eyes) were randomly divided into A and B groups. Thirty-one eyes (group A) were treated by excision of pterygium combined with autologous transplantation of superior limbal stem cells and use of mitomycin C. Thirty-one eyes (group B) were treated by excision of pterygium combined with autologous transplantation of inferior limbal stem cells and use of mitomycin C. Duration of uncomfortable symptom, healing time of corneal epithelia, and recurrence of pterygium were observed.Results　There were not statistically difference in the rate of pterygium recurrence between two groups (P>0.05). The difference of duration of uncomfortable symptom was statistically significance (P<0.05). There were not statistically difference in healing time of corneal epithelia between two groups (P>0.05).Conclusion　The autologous transplantations of superior or inferior limbal stem cells have the same effect in treating primary pterygium. The duration of uncomfortable symptom in the patients were treated by the transplantation of inferior limbal stem cells is shorter.]]> 2018/6/28 8:51:28 WU Yu,LI Min, CHEN Qi 820true ［Abstract］　Objective　To investigate the clinical efficacies of percutaneous vertebroplasty (PVP) in patients with osteoporotic vertebral compression fractures. Methods　One hundred and twenty eldely patients （145 vertebrae） of osteoporotic vertebral compression fractures of spine were treated with percutaneous vertebroplasty, relief of postoperative pain was observed. The amount of pain reduction was followed and quantified using the visual analog scale(VAS) before surgery, 3 d, 1 month, 3 months after operations.Results　All patients were followed up for 3 months, pain was completely relieved in 100 cases, partial relieved in 15 cases, unrelieved in 5 cases. Effective rate was 95.83%. The preoperative visual analogue scale was statistically significant when compared to postoperative time（P<0.05）, but no significant difference was shown bewteen postoperative time（P>0.05）. Conclusion　PVP is a safe and effective treatment in patients with osteoporotic vertebral compression fractures, which is effective in pain relief.]]> 2018/6/28 8:51:28 GAN Feng-ping,TAN Hai-tao,JIANG Jian-zhong, et al. 819true ［Abstract］　Objective　To explore the method and effect of neostigmine acupoints injection in adhesive intestinal obstruction.Methods　Ninety cases of adhesive intestinal obstruction were randomly divided into simple treatment group (n=30), surgery treatment group (n=30) and neostigmine acupoints injection treatment group (n=30). Simple treatment group received non-operative western medical treatment, including abrosia, anti-infection, fluid infusion, continuous gastrointestinal decompression, etc. Surgery treatment group recieved surgery after hospital admission. The third group received neostigmine acupoints injection besides ordinary treatment. Results　In neostigmine acupoints injection treatment group, 15 cases were cured with 80% effective rate and 16.7% relapse rate; in simple treatment group, 12 cases were cured with 80% effective rate and 33.3% relapse rate; in surgery treatment group, 19 cases were cured with 93.3% effective rate and 30% relapse rate. Compared with simple treatment group and surgery treatment group, average anus exhaust time and peristalsis recovery time of neostigmine acupoints injection group were shorter (P<0.01). Compared with simple treatment group, defecation time of neostigmine acupoints injection group was obviously shorter (P<0.01). Conclusion　Neostigmine acupoints injection reduces course of treatment. Many patients don′t have to undergo operation again, which saves a lot of treatment cost. This is an ideal method to treat adhesive intestinal obstruction, deserving to be applied to clinical treatment.]]> 2018/6/28 8:51:28 LAO Jing-mao 818true ［Abstract］　Objective　To investigate the feelings and the function of mastication of patients using the ERA attachment dentures with questionnaire.Methods　In selected 60 Kennendy type I defected patients, totally 35 ERA attachment dentures and 33 clasp removable partial dentures were made. Three months after treatment, personal feelings like fitness, the satisfaction and alteration of the function of mastication were investigated with questionnaire.Results　The ERA attachment dentures were compared with the clasp removable partial denture in terms of total satisfaction, cosmetic result, comfort degree, mastication, pronunciation, retention, and the food. The former was superior to the latter，there was significant difference in the above indexes between two kinds of denturts(P<0.05).Conclusion　The ERA attachment denture not only can satisfy the patients in the cosmetic result and the function，but also be superior to the clasp removable partial denture.]]> 2018/6/28 8:51:28 LIU Guang-xue,PAN Xiao-bo,PENG Li-hui,et al. 817true ［Abstract］　Objective　To study Giemsa′s dyeing form of human pneumocystis jirovecii(PJ).Methods　Giemsa staining method was used for smearing and dyeing of sputum and BALF PJ.The PJ forms were observed under microscope with oil immersion lens.Results　PJ in sputum showed about the size of 1.0～7.5 μm. Its cysts were round or oval.The cyst wall was light blue.Intracystic bodies were purple or purplish blue.Trophozoite cytoplasm was light blue.Its cytoplasm was purple or purplish blue.The cysts can be single,several,scattered, arranged in groups,or clusters.In the cysts arranged in groups there were different development stages of trophozoites.The difference of bug body size was bigger.Bug body form was diversiform.Typical mature cysts were very rare.Immature cysts and trophozoites were in the majority.Conclusion　Giemsa′s dyeing form of hunan PJ have more types than animal PJ.Often in immature cysts and trophozoites are in the majority in the film expression.]]> 2018/7/2 15:40:24 CHEN Jing-Jie,LI Yong,HE Han,et al. 816true ［Abstract］　Objective　To investigate the level of VEGF and nm23 expression in esophageal cancer and normal esophageal mucosa in Guangxi, and detect their relationships with clinical predictive indicators.Methods　The levels of VEGF and nm23 expression in 80 cases of esophageal cancer and 10 cases of normal esophageal mucosa were detected by SP immunohistochemical method.Results　VEGF positive expression rate in esophageal cancer was significantly higher than that of normal esophageal mucosa (P<0.05). VEGF positive expression rate had no significant correlation with the patient′s age, sex,and histological type(P>0.05),but was correlation with degree of the differentiation,tumors invade depth, clinical stage,and lymph node metastasis (P<0.05). nm23 positive expression in esophageal cancer significantly lower than that of normal esophageal mucosa (P <0.05). nm23 positive expression had no significant correlation with the patient′s age, sex,histological type, degree of the differentiation,and tumors invade depth (P>0.05),but it was correlation with lymph node metastasis and clinical stage (P<0.05).VEGF had negative correalation with nm23 (r=0.-0.520,P<0.05).Conclusion　The over-expression of VEGF and The lower-expression of nm23 were significantly correlated with the carcinogenesis of esophageal cancer in Guangxi.And they had negative affection with each other.]]> 2018/7/2 15:40:24 SU Nai-wei,FENG Xu,QIN Jia-Jin 815true ［Abstract］　Objective　To evaluate the long-time outcomes of gluteal muscle contracture with the procedure of traditional muscle and fascia release combined with rising the gluteus maximus attachments.Methods　One hundred and sixty patients were divided into two groups,the one treated with the traditional surgical procedure,the others received the procedure of traditional muscle and fascia release combined with rising the gluteus maximus attachments.At the latest follow-up,the improved abduction contracture of the hip were compared in two groups ;further more one outcome scoring was introduced to evaluate the long-time outcomes.Results　At the latest follow-up, the group underwent the traditional muscle and fascia release combined with rising the gluteus maximus attachments procedure had more improved abduction contracture and higher outcome scoring.Conclusion　Traditional muscle and fascia release combined with rising the gluteus maximus attachments procedure have a more satisfacted clinical outcome compared with traditional surgical procedure.]]> 2018/7/2 15:40:24 WANG Xian,QIU De-zan, YIN Dong, et al. 814true ［Abstract］　Objective　To evaluate the influence of individualized exercises on the abnormal heart rate variability (HRV) in type-2 diabetes patients.Methods　24-hour dynamic electrocardiogram (DEG) was used for detecting HRV of type-2 diabetes patients before and after the patients taking individualized exercises prescription.Results　There were 16 patients were included in this group and completed the 12 weeks individualized exercises prescription（aerobic exercise）.After the therapy from 8 to 12 weeks，the number of patients whose HRV were improved incresed than that before treatment and DCG analysis showed HF value incresed after treament.There was a significant statistical difference（P<0.05）.Conclusion　This study indicates that individualized exercises could improve cardiovascu1ar autonomic neuropathy in type-2 diabete patients.]]> 2018/7/2 15:40:24 MO YI，LIU Yu-tian，CHEN Qing-ping，et al. 813true ［Abstract］　Objective　To explore the effect of clinical application of TZ-FK660 gynecological comprehensive instrument on postpartum lactation,uterine involution,postpartun urinary retention.Methods　1 200 hospitalized parturients who had delivery in our hospital from June 2010 to June 2011 were randomly assigned to observation group(600 parturients) and control group (600 parturients).The observation group were treated by TZ-FK660 gynecological comprehensive instrument,and the control group by traditional method.Therapeutic effect between two groups were compared.Results　After treatment,the observation group was superior to the control group in promoting milk secretion,relieving breast pain,reducing postpartum urinary retention.The differences had statistical significance（P<0.05）.Conclusion　TZ-FK660 gynecological comprehensive instrument has obvious role in promoting postpartum lactation,reducing postpartum hemorrhage,promoting uterine involution,treating postpartum urinary retention.It is worthy of clinical application.]]> 2018/7/2 15:40:24 WANG Guo-fen,BAO Chun-jiu,LI Chun-hua,et al. 812true ［Abstract］　Objective　To investigate the relationship between Thr394Thr and Gly482Ser of the PGC-1α gene and type 2 diabetes in a family-based association study in the southern Chinese population.Methods　Three hundred and fifty patients and their first-degree relatives were recruited in our study. Their blood specimens were collected to extract the genomic DNA. The genotypes of the polymorphisms Thr394Thr（G/A）and Gly482Ser（G/A）of the PGC-1α gene were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)and direct sequencing. The results were analyzed by transmission disequilibrium test (TDT) and haplotype relative risk (HRR).Results　HRR revealed that the 482A allele was more often transmitted to patients (χ²=7.2170，P=0.0072，HRR=1.4496). TDT analyses revealed that 394Thr（A）-482Ser（A）haplotype was significantly more transmitted from heterozygous parents to patients than expected (χ²=33.160，P=0.002). There was a linkage disequilibrium between the haplotype and type 2 diabetes（χ²=4.6841，P=0.0292）.Conclusion　The co-variation of 394A-482A may increase the risk of diabetes in the Chinese population.]]> 2018/4/27 8:24:40 HUANG Zhong, ZHONG Mei,HUANG Qun, et al. 811true ［Abstract］　Objective　To explore the clinical significance of Clara cell secretory protein(CCSP)in children with community acquired pneumonia(CAP). Methods　Thirty-four children with CAP and twenty-two healthy controls were enrolled.Venous blood samples and sputum samples were collected in CAP children,and blood samples alone were obtained in control subjects.After 10～14 days of treatment,blood samples were obtained in children with CAP without clinical symptoms and signs. The levels of CCSP were measured in sera and sputum by enzyme-linked immunosorhent assay（ELISA）.Results　Children with CAP,compared with controls,had significantly lower concentration of CCSP in sera(P<0.01).After recovery from pneumonia,the levels of CCSP in children with CAP increased to normal levels(P>0.05); In both severe CAP group and mild CAP group,the levels of sera CCSP in CAP children were significantly lower than those in control group,especially those in mild CAP group, but there were no difference between two CAP groups(P>0.05); In severe CAP group,the levels of CCSP in sputum were significantly lower than those in mild CAP group(P<0.01); In mild CAP group,there were significant positive correlation of CCSP levels between sera and sputum (P<0.01).Unfortunately,in severe CAP group,there were no correlation of CCSP concentration between sera and sputum(P>0.05).Conclusion　CCSP may play a protective role in the pathogenesis and development of CAP.And the CCSP levels may preferably evaluate the state of children with CAP.]]> 2018/4/27 8:24:40 WEN Zhi-hong,HU Qiong-yan,ZHOU Wei-ya,et al. 810true ［Abstract］　Objective　To explore the modulation of endocannabinoid system on visceral sensation in rat.Methods　Twenty-four rats were divided into 4 groups:control, acute restraint stress with vehicle, acute restraint stress with agonist,acute restraint stress with antagonist. The acute restraint stress model of rat were established, vehicle,1319ACEA and SR141716A were administrated by intraperitoneal injection, then colorectal distension(CRD) were conducted and abdominal electromyogram in response to CRD was recorded. The expression of nNOS in ileocecum and colon mucosa was assessed by western blotting.Results　The frequence of electrical activity of abdominal extemal oblique muscle of rat model of acute restraint stress with administration with SR141716A intraperitoneally significantly higher than those of other 3 groups in CRD of 60mmHg and 80 mmHg ［（158.0±3.20）,（119.57±13.58）,（131.77±20.61）,（102.86±34.96）；（219.01±12.97）,（178.83±22.16）,（181.95±32.72）,（153.77±38.10）,P<0.05］；The expression of nNOS in mucosa of ileocecum and proximal and distal colon in rat model of acute restraint stress was significantly lower than those in control ［（0.58±0.0） vs （70.71±0.08）,（0.54±0.10） vs （0.68±0.08）,（0.59±0.08） vs （0.72±0.09）, P<0.01］.Conclusion　The endocannabinoid system may modulate visceral hypersensitivity by CB1 receptor. The down regulation of activity of nNOS was associated with visceral hypersensitivity.]]> 2018/4/27 8:24:40 LIANG Lie-xin, ZUO Guo-wen, ZHANG Guo, et al. 809true ［Abstract］　Objective　To explore the effect of continuous vacuum sealing drainage (VSD) technique in the prevenction of wound infection in acute soft-tissue trauma.Methods　Eighty cases of acute traumatic wounds caused by various reasons were divided into two groups at random. Of them 40 cases were treated by covering wonds with continuous VSD after general wound debridement (treatment group,groupA) and the other 40 cases were treated by regular debridement of the wound followed by covering biological dressing A (control group,group B).The specimen was taken form injured area for bacterial culture on preoperative and 7th postoperative day; the wound swelling degree were observed within 7 days of trauma.According to the condition of wounds patients in tow groups were choosed different surgical methods for wound repair 7days later.Results　In group A the positive bacterial cultures was found in 6 cases,the wound infection rate was 15.0%,which was significantly lower than the group B (22 cases,55%)，(χ²=14.066，P=0.000).Wound swelling degree of group A tend to be slighter than that of group B(u=-2.035， P=0.042). The average time of wound healing in group A was significantly shorter than that in group B(t=-3.466,P=0.001).Conclusion　Using continuous VSD technique in acute soft tissue trauma can reduce the wound infection rate, and facilitate wound healing.]]> 2018/4/27 8:24:40 MAO Qing-long, SHOU Jia-bao,TANG Zhe-ming,et al. 808true ［Abstract］　Objective　To evaluate the expression of lymphatic vessels of in primary foci of nasopharyngeal carcinoma(NPC) after intratumoral injection of recombinant human p53 adenovirus (rAd-p53).Methods　Sixty-three patients with advanced nasopharyngeal carcinoma were randomly divided into 2 groups. Treatment group (n=32) received rAd-p53 intratumoral injection,concurrent radiotherapy and chemotherapy. The control group (n=31) received concurrent radiotherapy and chemotherapy. Clinical data were collected, and before and after intratumoral injection of rAd-p53 nasopharyngeal tumor biopsy specimens were collected. Two-step immunohistochemical detection was used to test D2-40 expression in primary foci of NPC.Results　After treatment,D2-40 expression was significantly higher than that before treatment in two groups(P<0.05 or P<0.01). The difference of D2-40 expression between two groups was not statistically significant (P>0.05).Conclusion　rAd-p53 may inhibite lymph node metastasis of nasopharyngeal carcinoma by some mechanism.]]> 2018/4/27 8:24:40 JIANG He, LAN Gui-ping,WENG Jing-jin,et al. 807true ［Abstract］　Objective　To investigate the effect of two ventilation strategies on lung compliance and blood IL-6,IL-8 in elder patients during abdominal surgery.Methods　Forty ASAⅠ～Ⅱ elder patients,aged >60 years, scheduled for selective abdominal operation under general anesthesia were divided into two groups randomly: group A(VT 7ml/kg PBW) PEEP was 5 cmH₂O； group B(VT 12 ml/kg PBW).Pressure controlled ventilation was performed after tracheal intubation, P_ETCO₂ was maintained at normal range through respiratory rate adjusted（RR）.HR,MAP,SpO₂,PET CO₂,CL were monitored continuously and recorded at 5 min(T0),30 min(T1),1 h(T2),3 h(T3) after tracheal intubation；the serum levels of the interleukins IL-6 and IL-8 were monitored at 5min and 3 h after ventilation strategies.Results　There were no significant differences in HR,MAP and P_ETCO₂ between the two groups（P>0.05）; and CL in group B was lower than that in the group A(P<0.01). Levels of the blood interleukins IL-6 and IL-8 in group A were decreased compared with the B group at 3 h after ventilation strategies (P=0.000, P=0.002).Conclusion　For elder patients during abdominal surgery, two mechanical ventilotion strategies can incr-ease the levels of blood IL-6 and IL-8 and decrease CL,but these change are less in mechanical ventilation with low tidal volume than that in conventional ventilation with high tidal volume.]]> 2018/4/27 8:24:40 HUANG Zhong-hua,ZHU Wei-lin,QIN Dan-dan 806true ［Abstract］　Objective　To evaluate the efficacy of saccharomyces boulardii (S. boulardii) in treatment of children with acute diarrhea.Methods　One hundred hospitalized children with acute diarrhea aged from 2 months to 4 years in our hospital from January 2011 to December 2011 were studied. They were randomly divided into treatment group (n=50) and control group (n=50).Control group was treated with Medilac-Vita, and treatment group was treated with Saccharomyces boulardii powder combined with compound lysine hydrochloride and zinc gluconate Granules.Results　The total effective rate, cure rate and symptom improvement time in experimental group were significantly better than those in control group (P<0.05).Conclusion　On base of basic treatment, Saccharomyces boulardii powder combined with compound lysine hydrochloride and zinc gluconate granules in treatment of children with acute diarrhea can significantly enhance clinical effect and reduce the recovery time, so it deserved application and promotion in clinical work.]]> 2018/4/27 8:24:40 LI Xing-hong, PANG Xiao-jun, LIU Ying-mei, et al. 805true ［Abstract］　Objective　To explore the role of IL-8 and VEGF in the pathogenesis and development of nasopharyngeal non-keratinizing carcinoma(NPC). Methods　Expression of IL-8 and VEGF was examined by S-P immunohistochemistry in 80 NPC archival paraffin-embedded tissue specimens and 15 controlled chronic nasopharyngitis mucosa. The relationship between clinicopathological features and expression of IL-8 and VEGF were also analyzed.Results　Expression rates of IL-8 and VEGF in NPC cases and were 67.5%（54/80） and 65%（52/80）, respectively, versus 20%（3/15） for both IL-8 and VEGF in chronic nasopharyngitis. The expression rates of the IL-8 and VEGF in NPC were significantly higher than that in chronic nasopharyngitis (P <0.05). There was positive correlation between the expression of IL-8 and VEGF（r=0.328, P <0.05）. No correlation was found between the expression of IL-8 and VEGF with patients′ clinicopathological features as to gender, age, lymph node metastasis, histotype and TNM stages.Conclusion　IL-8 and VEGF are over-expressed in NPC, and maybe cooperate as angiogenesis factors in the pathogenesis and development of NPC.]]> 2018/5/14 8:34:41 LI Hua, WEI Min-yi, LIANG Xiu-jiu, et al. 804true ［Abstract］　Objective　To investigate the concentrations and clinical significance of Toll-like receptor 2 and 4 in pleural effusions with various etiologies. Methods　Pleural effusion samples were obtained from 81 patients who presented to the respiratory institute (32 with tuberculous pleural effusion，28 with malignant pleural effusion and 21 with bacterial pleural effusion). The pleural effusion and serum levels of TLR-2 and TLR-4 were determined by sandwich enzyme- linked immunosorbent assays (ELISA).Results　（1）The concentrations of TLR-2 in tuberculous pleural effusion (22.19±2.12 )ng/L was higher than that in malignant group (14.50 ± 1.93)ng/L, in bacterial group (13.99±2.36)ng/L （P<0.05）. But there were no statistical significance between the malignant group and bacterial group （P>0.05）.（2）In serum，The concentrations of TLR-4 in bacterial group (5.34 ± 2.13)ng/L was higher than that in malignant group(0.10±0.06)ng/L, in tuberculous group (1.54 ± 1.09)ng/L （P<0.05）.（3）Between pleural effusion and serum， there were statistical significance in the three groups (P<0.05).（4）The concentrations of TLR-2 and TLR-4 in tuberculous pleural effusion were positively correlated (P<0.05).Conclusion　Pleural TLR-2 may be helpful for the differential diagnosis of tuberculous pleural effusion and no- tuberculous pleural effusion. TLR-2 and TLR-4 maybe play an important role in the pathogenesis of tuberculous pleurisy.]]> 2018/5/14 8:34:42 WU Yan-bin, HUANG Lu-ying, FENG Ting-mei, et al. 803true ［Abstract］　Objective　To investigate the expression of total IgE in nasal polyps in patieats of chronic rhinosinusities with nasal polyg(CRSWNP) and its relationship with hypersensitivity reaction type I mediated by IgE.Methods　The expression of total IgE protein in nasal polyps tissue in 40 cases of CRSWNP(20 cases with and 20 cases without allergic rhinitis) nasal polyps tissue(the CRSWNP group) and 20 middle turbinate tissue samples(the control group) were examined by immunohistochemistry.All cases were scored with Lund-Mackay CT system in CRSWNP group and analyzed statistically.Results　The positive stainning cells of total IgE protein in nasal polyps tissue in CRSWNP group was (14.65±3.34), which was significant higher than that in control group （3.40±1.27）(P<0.01),the expression of total IgE in nasal polyps with and without allergic rhinitis in CRSWNP was 14.69±2.67 and 14.58±4.31 respectively,no significant statistical difference in the expression of total IgE was found between them.There was a positive correlation between the expression of total IgE and Lund-Mackay CT system scores （12.35±4.26） in CRSWNP group (r=0.464，P<0.05).Conclusion　The overexpression of total IgE palys a role in CRSWNP,that may be different with hypersensitivity reaction type I mediated by IgE.]]> 2018/5/14 8:34:42 HE Ning, LIANG Jian-ping,CHEN Jun,et al. 802true ［Abstract］　Objective　To study the most effective treatment for children and adolescents depression.Methods　The open mode of randomized controlled study (RCT) was conducted. The children and adolescents (n=122) at the age of 12～18 years who were complied with depression diagnosis standard were divided into the 3 groups: cognitive behavioral therapy(CBT) + Fluoxetine group (n=42); CBT group (n=43) and Fluoxetine group (n=37). Before treatment and after treatment，the Global Assessment Function (GAF), the Global Assessment of Relational Functioning (GARF), Clinical Global Impression (CGI-S)，and before treatment, 2nd w, 4th w, 6th w, 8th w and 12th w after the beginning of treatment, the Hamilton depression scale (HRSD), Depressed mood thermometer (T-S) and Irritable mood thermometer (T-I) were conducted to evaluate the treatment effect.Results　HRSD, T-I and T-S scores of 3 groups were reducing gradually with the time of treatment were found, and before and after treatment were significantly different （P<0.01）, but within the three groups were not different. Before and after treatment CGI, GARF, GAF scores of the 3 groups were significantly different （P<0.01）. Before and after the treatment, CGI, GARF, GAF scores of the CBT group were significantly different with the other group （P<0.01）.Conclusion　The international general CBT treatment guidelines can be used for children and adolescents depression. The CBT, CBT + Fluoxetine and Fluoxetine alone could be an effective treatment for children and adolescents depression. CBT seemed to be most effective therapy for children and adolescents depression.]]> 2018/5/14 8:34:42 WU Xin, HUANG Ling, SHI Jie, et al. 801true ［Abstract］　Objective　To investigate the risk factor of malignant solitary pulmonary nodule(SPN).Methods　A monovariate analysis was used for exploring the correlation between clinical characteristics and pathological characteristics in 120 patients with SPN and then a multivariate analysis was performed in the factors with significant difference.Results　Sixty-four patients postoperatively were proved to be malignant SPN.There was significant correlation between four factors(namely smoking,nodule size,age,image characteristics) and pathological characteristics in monovariate analysis.However only two factors(age and image characteristics)were left in multivariate analysis.Conclusion　Smoking,nodule size,age and image characteristics,especially age and image characteristics,are the risk factors of malignant SPN. With a overall analysis for these factors,doctors can improve diagnosis accuracy of SPN.]]> 2018/5/14 8:34:42 YANG Shi-xiong, WANG Bin-qiang,ZENG Jian-ye,et al. 800true ［Abstract］　Objective　To observe the prevalence of hypertension and associated risk factors in the Fishing populations of Tieshangang of Baihei City.Methods　Residents over 20 years of age were surveyed by a stratified randomized cluster sampling.Investigation of the prevalence of hypertension and risk factors were performed.Results　The prevalence of hypertension in the fishing populations was 14.3%. The rate of men and women were 16.0% and 12.4%(P<0.01). The single factor analysis showed that age, sex, marriage, cultural degree, household history, overweight, and so on were related with the prevalence of hypertension (P<0.05). The logistic regression analysis showed that age, smoking, family history of hypertension, physical activity, culture degree was significantly related with the fishing population prevalence of hypertension. In which age, smoking, family history of hypertension, lower physical activity were risk factors. And high cultural degree, low probability of suffering from hypertension had protective effect.Conclusion　Tieshangang district prevalence rate of hypertension was belong to the lower level, It is necessary to improve the hypertension knowledge publicity in the coastal areas, and prevent hypertension from the diet and lifestyle and so on.]]> 2018/5/14 8:34:42 LI Ren-fu, SU Fu-wo, XIAO Xin, et al. 799true ［Abstract］　Objective　To evaluate the curative effect of wufangsan plaster therapy combined with herbal oral administration,TCM hot medicated compress of the back and functional exercise in the treatment of thoracolumbar fracture without neurologic deficit.Methods　Two hundred and twenty-five patients with thoracolumbar fracture without neurologic deficit treated in our hospital from September 2008 to August 2010 were divided into three groups according to different treatments, including plaster therapy group(47 cases), TCM(Traditional Chinese Medicine) hot medicated compress group (43 cases), and combined therapy group (135 cases),then the curative effectiveness was compared by the same evaluation criteria.Results　About improvement of the back pain, there was significant difference between plaster therapy group and combined therapy group on 3rd and 7th day after treatment（P<0.05 or P<0.01）,but there was no significant difference on 10th day after treatment（P>0.05）; and there was significant difference between TCM Hot medicated compress group and combined therapy group at every time points(P<0.05 or P<0.01)but no significant difference between plaster therapy group and TCM hot medicated compress group（P>0.05）. The clinical effect was obvious difference between combined therapy group and plaster therapy group or TCM hot medicated compress group(P<0.05 or P<0.01). Some patients in 3 groups suffered from skin allergies but there was no significant difference between combined modality therapy group and plaster therapy group or TCM hot medicated compress group（P>0.05）.Conclusion　As for thoracolumbar fracture without neurologic, the curative effect of traditional Chinese combined therapy is better than that of plaster therapy or TCM hot medicated compress therapy, but some patients may suffer from skin allergies.]]> 2018/5/14 8:34:42 XU Jian-wen, YIN Li-jun, ZHONG Yuan-ming, et al. 798true ［Abstract］　Objective　To explore the main MRI signs of knee joint and the clinical stages of juvenile idiopathic arthritis (JIA) in children.Methods　Twenty-four children who had been clinically diagnosed as JIA and with the symptom of knee pain and swelling were selected to observe their MRI signs of knee joint and the corresponding clinical stages.Results　With MRI enhanced scan, there were 17 cases showed synovial thickening of suprapatellar bursa and a significantly arc strengthend in 24 children; 11 cases showed the pannus formation in the 17 children (11/17) of them, 6 cases who were in the acute or subacute period of activity showed small nodular strengthend (6/17), 3 cases in the active or delayed period (3/17) showed small nodule-like strengthened focus and the cord-like non-strengthened shadow, 2 cases who were in the delayed or stable period (2/17) only showed the cord-like non-strengthened shadow. All of 24 children showed suprapatellar bursa effusion. The synovial thickness of the thickest layer of suprapatellar bursa and the suprapatellar bursa effusion could reflect activity of JIA, and there was correlation between the two indexes and clinical stages.Conclusion　There was certain characteristic of MRI signs of the knee joint in JIA children, which could reflect the different clinical periods of JIA disease.]]> 2018/5/14 8:34:42 WANG Yi, LI Rui 797true ［Abstract］　Objective　To explore the clinical characteristics, diagnosis and treatment methods of small bowel stromal tumors.Methods　Twenty-six patients with small bowel stromal tumors was collected from 2004 to 2010,and the clinicopathologic data and survival data was reviewed retrospectively.Results　Of 26 patients with small bowel stromal tumors, there were 15 males and 11 females, the median age was 51 years old. The most common clinical manifestation was finding of tumor in abdomen,abdominal pain and melena.Tumor originated from duodenum, jejunum, ileum were 3, 18 and 5 patients, respectively.Two patients got infiltration of adjacent organs, while 4 patients had distant metastasis. The positive rates of immunohistochemical staining of CD117,CD34, Desmin, Actin, S-100, NSE were 96.2%, 76.9%, 3.8%, 23.1%, 0.0%, 26.9%, respectively. Twenty-two patients received radical resection and were followed-up, ended up with 10 deaths, and the mean survival time was 50 months. There were no significant differences in immunohistochemical staining, clinicopathologic factors and prognosis between small bowel and gastric stromal tumors (P>0.05).Conclusion　Small bowel stromal tumors have similar clinical characteristics and prognosis to gastric stromal tumors. It is important to choose rational auxiliary examinations preoperatively. Radical resection may be the mayor treatment, and long-time following-up should be proposed after operation.]]> 2018/5/14 8:34:42 HE Chun-gang,HUANG Qin-yuan,WU Hong-gen,et al. 796true ［Abstract］　Objective　To investigate the effect of color Doppler flow imaging in monitoring the occurrence of intraductal thrombosis after deep venous catheterization in severe patients.Methods　Seven seriously ill patients with normal coagulantion function in EICU of the people′s hospital of Guangxi Zhuang Autonomous Region were chosen. CRP,D-D,PLT,PT,APTT,FIB and TT of patients were tested before deep venous puncture. 48 hours later,The aforementioned items were tested again and the deep venous catheters were monitored by colour Doppler flow imaging. Successionally the deep venous catheters were pulled out and the intraductal thrombosis were tested by pathological examination. All the test results and the relevant factors of intraductal thrombosis after deep venous catheter was inserted were statistically analysed.Results　The intraductal thrombosis in 7 patients were verified through pathological examination after deep venous catheter were inserted. The statistical difference of the test result of CRP,D-D,PLT,PT,APTT,FIB,TT were not significant before and after deep venous venipuncture (P> 0.05).yet,the intraductal thrombosis in 6 patients could be monitored by color Doppler flow imaging.Conclusion　It is a good means to use Doppler flow imaging to monitor the occurrence of intraductal thrombosis in deep venous catheterization.]]> 2018/5/14 8:34:42 DENG Da-zhi,HUANG Xiang-hong 795true ［Abstract］　Objective　To compare the degree of oral mucosal lesions of mouse tongue epithelial carcinoma model induced by four-nitroquinoline l-oxide (4-NQO) in drinking water in different dosing schedules.Methods　Seventy balb/c mice were randomly assigned into two groups: the experimental group (n=60) and the control group (n=10). The experimental groups were given 200 mg/L 4-NQO in drinking water for 8, 14, 20 weeks (n=20), respectively, and then given ordinary water to 45 weeks. The control group was given ordinary tap water. All the mice were killed in 45 weeks, then their tongues and submandibular lymph nodes were removed for histologic study.Results　With the time prolonged, the changes including leukoplakia, ulcer appearances, papillary hyperplasia were gradually occurred on the mucosa of the experimental groups. Their corresponding histopthological results ranged from hyperplasia, mild dysplasia(MiDP), moderate dysplasia(MoDP), severe dysplasia (SDP), In situ carcinoma(ISC) to invasive carcinoma(IC) along with the increasing administration time. The tongue of mice which were given 4-NQO for 8 weeks showed hyperplasia (70.6%), MiDP/MoDP and SDP (29.4%); The tongue of mice which were given 4-NQO for 14 weeks showed MiDP/MoDP and SDP (88.2%), ISC(11.8%); The tongue of mice which were given 4-NQO for 20 weeks showed IC(100% )and 100% submandibular lymph nodes metastasis were found. Compared with before administration, the body weight of mice of 14, 20-week groupss significantly reduced (P<0.05), while there were no significantly changes of the body weight of mice of 8 weeks and control groups (P> 0.05).Conclusion　4-NQO could be given in drinking water to induce tongue epithelial carcinoma model at the same dose in different dosing schedules. With the increasing administration time, the higher rate of tongue precancerous lesions and the tongue epithelial carcinoma occured, but the body weight of mice significantly reduce.]]> 2018/5/14 8:35:38 LI Ming-wu, LI Jing, YU Da-hai,et al. 794true ［Abstract］　Objective　To investigate the effeet of statin pretreatment on the incidence of plaque rupture and unstable angina pectoris （UAP）detected by intravascular ultrasound(IVUS) in patients with coronary heart disease（CHD）.Methods　A retrospective case control study was conducted. Prevalence of abnormal blood fat content, statin treatment rate, and attainment rate of low density lipoprotein-cholesterol(LDL-C) lowered to expected level in 62 patients with CHD were analyzed. Relationship between statin therapy and level of LDL-C were analyzed by multivariate general linear factorial analysis. The incidence of unstable angina pectoris (UAP) was compared in patients with CHD receiving statin therapy in all groups. IVUS interrogation was performed before percutaneous coronary intervention.Results　Prevalence of abnormal LDL-C was 96.4%（27/28）in CHD patients of non-statin therapy group, and 46.4%（13/28）of patients with CHD should receive statin therapy but did not. Attainment rate of lowering of LDL-C to expected level in statin therapy group was 26.5%（9/34）.LDL-C level (mmol/L) was significantly lower in statin therapy group than in non-statin therapy group（2.457±0.802 vs 3.218±1.130, Z=-9.760,P=0.001）,and incidence of UAP was significantly lower in statin therapy group than in non-statin therapy group（32.3% vs 71.4%, χ²=34.491,P=0.001）. There was no difference between patients with and without attaining the expected low value of LDL-C in statin therapy group（33.3% vs 32%, χ²=0.002,P=0.968）. Plaque ruptures were detected in 30 patients(30/62). Patients with statin pretreatment(n=34) had a lower incidence of plaque rupture than those without(38.2% vs 60.7%, χ²=7.3, P=0.007). Positive remodeling was also lower in patients with stain pretreatment than those without（29.4% vs 46.4%, χ²=3.06, P=0.04）.Conclusion　Incidence of UAP was significantly decrease after statin treatment in patients with CHD. It should be considered as the key treatment in CHD. Statin pretreatment before onset of UAP is associated with a lower plaque rupture, suggesting that the prevention of plaque rupture may be a crucial mechanism underlying clinical benefits associated with statins.]]> 2018/5/14 8:35:38 WANG Hong, LIN Ying-zhong, LIU Ling, et al. 793true ［Abstract］　Objective　To evaluate the influence of functional exercise of the lumbodorsal muscles on the vertebral body height of primary thoracolumbar vertebral osteoporotic fracture.Methods　Ninety patients with primary thoracolumbar vertebral osteoporotic fracture were treated with functional exercise of lumbodorsal muscles and with the chinese and western medicine as the adjunct treatment, aiming at observing the changes of vertebral body height.Results　There was no adverse events to be found in the course of treatment. After 1 month, the average vertebral height restoration of the anterior edge, middle and posterior edge were（2.92± 2.01）mm,（2.36±1.89）mm and（1.09±1.03）mm.When comparing pre-treatment with after treatment, the anterior edge, middle edge and posterior edge vertebral height restoration were different(P<0.05).After 3 months, there were not evident of the loss of vertebral body height.Conclusion　Functional exercise of the lumbodorsal muscles for primary thoracolumbar vertebral osteoporotic fracture have obvious short-term efficiency, but long-term effect needs further follow-up observation.]]> 2018/5/14 8:35:38 LI Yu-chun, XU Jian-wen, HUANG Yong-guang, et al. 792true ［Abstract］　Objective　To study the diagnosis and treatment of pulmonary aspergillosis.Methods　The case histories of 53 patients with pulmonary aspergillosis(24 with aspergillus ball, 21with chronic and necrotizing pulmonary aspergillosis, 8 with aspergillus ball complicated with chronic and necrotizing pulmonary aspergillosis), pathologically confirmed from January, 2008 to December, 2010 after pneumectomy were retrospectively analyszed.Results　There were 39 cases with underlying disease, of which the most common was tuberculosis and the second was bronchiectasis, lacking specificity of clinical manifestations. Imaging examination showed the diseased region mainly located in the upper part of lung, a total of 37 cases; The main features lesions were pulmonary lobe and segment′s infiltration with cavity, a total of 43 cases; air crescent, 7 cases; cavernous nodule with internal attachment or irregular lump opacities, 27 cases. All patients were pathologically diagnosed after pneumectomy, without antifungal treatment, 5 cases with recurrence of them were those who suffered chronic and necrotizing pulmonary aspergillosis and multiple pulmonary lobes lesion of double lungs.Conclusion　Lacking specificity of clinical manifestations, it is easy to be misdiagnosed tuberculosis for aspergillus ball and chronic and necrotizing pulmonary aspergillosis. Air crescent shows its significance on predictive diagnosis, and making a definite diagnosis requires pathological foundation. The first choice of aspergillus ball can come to surgical treatment, while chronic and necrotizing pulmonary aspergillosis can turn to medication or surgical treatment. With complete lesion resection and without underlying disease, those who have immunological function repression can not need the antifungal treatment.]]> 2018/5/14 8:35:38 LI Wu-ming,WEI Ming,MENG Zhi-Hao,et al. 791true ［Abstract］　Objective　To study the characteristics of poisons resulting in acute poisoning in Guangxi.Methods　The study was performed by using the four-grade classificatin of poisons and the distribution of poisoning cases.Results　The poisons resulting in poisoning of 6 005 cases were 532 kinds. In these poisons, there were 17 kinds resulting in poisoning of 62.38% cases,and 515 kinds resulting in poisoning of 37.62% cases. With the classification, the poisons discovered in survey were approximately classified,and the the main characteristics of substances were reported.Conclusion　There were more kinds of poisons in guangxi, and more concentrative distribution of poisoning cases. The influencing factors and changing law in the distribution of poisons are made further investigation.]]> 2018/5/14 8:35:38 HU De-hong, JIANG Dong-fang,LIU Qing-hua 790true ［Abstract］　Objective　To investigate the feasibility of pig model of liver transplantation with the hepatic artery reconstruction by the donor′s aorta.Methods　Ten miniature pigs were chosen, 5 pigs as donor, the other 5 pigs as recipient. The part of the aorta with the artery system of the donor was kept when retrieval of donor liver and the artery anastomosis by the donor′s aorta to the recipient aorta was performed when liver transplantation.Results　The liver transplantation of the all 5 cases was successful. The normal yellow bile was seen before the anastomosis of bile duct and 5 cases pigs were alive 24 hours after the operation.Conclusion　The feasibility of pig model of liver transplantation with the artery anastomosis by the donor′s aorta to the recipient aorta was confirmed and the study is a good base for deeper study of the research of liver transplantation with the artery anastomosis by the donor′s aorta to the recipient aorta.]]> 2018/5/14 8:35:38 LIU Tian-qi,PAN Jin-fei,HUANG Yu,et al. 789true ［Abstract］　Objective　To observe the effect of Astaxanthin capsula in the treatment of knee osteoarthritis.Methods　Sixty patients were randomly divided into treatment group and control group,treatment group received Astaxanthin capsula (1 capsules,2 times daily),the control group received Finbid (400 mg,2 times daily ),for 60 days.Results　The total effective rate of treatment group and control group were 66.67% and 83.33%. Compared with the control group, the difference was statistically significant (P>0.05). The pain relief time was longer and the maintenance time of pain relief was shorter after treatment (all P<0.01).After treatment, the knee function in two groups were improved (all P<0.01); the treatment group was superior to the control group in improvement of knee function (P<0.05).Conclusion　Astaxanthin capsula in the treatment of knee osteoarthritis shows better efficacy.]]> 2018/5/21 9:01:32 PEI Ling-peng,BAI Yan,ZHENG Ling-ling, et al. 788true ［Abstract］　Objective　To analyze the status and variation trend of the sexual character development and the age of spermatorrhea in rural youngsters of Guangxi.Methods　The survey was carried out in rural youngsters of Guangxi, aged from 12 to 16 years. The first spermatorrhea time were asked. Testiular volume, penile length and diameter were measured. Pubic hair were observed and divided into stages. Adam′s apple were examined.Results　The average age of first spermatorrhea is （14.52±0.08） years old, and the secondary sexual character development data in different age from 12 to 16 years old were collected, which including testiular volume, penile length and diameter, adam′s apple development,the obtioned data was similar to the domestic related reports.Conclusion　The overage age of the first spermatorrhea in rural youngsters of Guangxi was similar to that of national average age.Their age of starting puberty was similar to other reports.]]> 2018/5/21 9:01:32 TAN Da-xian, MO Yi, XIE Dan-ni, et al. 787true ［Abstract］　Objective　To investigate the effect of three ventilation strategies on lung compliance and oxygenation in elder patients during abdominal surgery. Methods　Sixty ASA Ⅰ～Ⅱ elder patients,Aged 60～81 years, scheduled for selective abdominal operation under general anesthesia were randomly divided into three groups: group LV( VT 7 ml/kg PBW)； group LV+PEEP( VT 7 ml/kg PBW, PEEP 5 cmH₂O)； group C (VT 12 ml/kg PBW).Pressure controlled ventilation was performed after tracheal intubation, P_ETCO₂ was maintained at normal range through respiratory rate adjusted（RR）.HR，MAP，SpO₂， P_ETCO₂，Ppeak,Pplat, CL were monitored continuously and recorded at 5 min(T0),30 min(T1),1 h(T2),3 h(T3) after tracheal intubation；Arterial blood gas analysis were performed at same point. Results　Sixty patients were analyzed. There were no significant differences in HR, MAP. P_ETCO₂ and operation methods among the three groups（P>0.05）. Mechanical ventilation with low tidal volumes and low tidal volumes plus PEEP led to lower airway pressures (Pplat and Ppeak, respectively) compared with the C group(P=0.001,P=0.012;P=0.006,P=0.011), and improved CL（P=0.004;P=0.001） and PaO₂（P=0.001; P=0.001） in compared with the C group; but CL and PaO₂ were decreasing during mechanical ventilation with low tidal volumes (P=0.003;P=0.002) and low tidal volumes plus PEEP(P=0.001;P=0.002).Conclusion　Mechanical ventilation with low tidal volumes improves CL and PaO₂ in elder patients during abdominal surgery, but not to prevent fully CL and PaO₂ from decreasing due to mechanical ventilation,and not to improve these with low tidal volumes adding to low PEEP.]]> 2018/5/21 9:01:32 ZHU Wei-lin, HUANG Zhong-hua,ZHANG Xue-gang, et al. 786true ［Abstract］　Objective　To investigate the impact of surgical method on judging the operative safety and the impact of patient selection on the effect of surgery in patients with primary hepatocellular carcinoma associaled with portal hypertension and hypersplenism.Methods　Clinical data of 60 patients with primary hepatocellular carcinoma associated with portal hypertension and hypersplenism treated in our hospital from May 2005 to Novenber 2011 were retrospectively analyzed, including 30 patients treated by the esophagus transection portal- azygos disconnection combined with liver and spleen resection, 30 patients treated by simple liver resection. Between two groups the liver function, the degree of cirrhosis, preoperative and postoperative changes in blood and liver function, postoperative complications, and other indicators were compared, for determining the safety of surgery and the impact of patient selection on surgical effect.Results　Sixty patients were followed up for 5 months to 5 years,with the average of (12.3 ± 2)months, In the treatment group 20 cases were cured, total effective rate was 96.7%, recurrence rate was 16.7%;in control group were 15 cases, total effective rate was 80.0%, the recurrence rate was 30.0%(P <0.05),in control group, there was no significant difference in preoperative and postoperative platelet and WBC; in treatment group, platelets and WBC after treatment was significantly higher than that before treatment. 5-year disease-free survival after the treatment in the treatment group and control groups were 10 cases (33.3%) and 5 casese (16.7%) respatively, the difference was significant (P <0.05).Conclusion　For patients with primary hepatocellular carcinoma associaled with portal hypertension and hypersplenism, concomitant esophageal transection patal- azygos disconnection combined with resection of the liver and spleen is feasible.]]> 2018/5/21 9:01:32 LAO Jing-mao 785true ［Abstract］　Objective　To discuss the treatment regimens of AIDS complicated by penicilliosis marneffei, improve its diagnosis and treatment level, and reduce mortality.Methods　Sixty-two patients with AIDS complicated by penicilliosis marneffei were divided into 2 groups (10 weeks group and 12 weeks group,with 31 patients in each group）.Two groups were given itraconazole,amphotericin B and anti-HIV treatment.The course of treatment in 10 weeks group was 10 weeks, that in 12 weeks group was 12 weeks.The relapse rate and mortality were compared between two groups.Results　There were no statistically difference in the relapse rate and mortality between the 10 weeks group and 12 weeks groups (P>0.05).Conclusion　The therapentic effect of two groups of AIDS compliated by penicilliosis marneffei are similar.]]> 2018/5/21 9:01:32 OU Ru-zhi, LU Xiang-chan, HUANG Shao-biao,et al. 784true ［Abstract］　Objective　To observe the effects of clinical applications of combined spinal- epidural analgesia with ropivacaine and fentanyl in labor analgesia.Methods　Forty pregnant women as antalgic group which received combined spinal- epidural analgesia with ropivacaine and fentanyl in labor analgesia, compared with 40 homogeneous pregnant women as control group. The labor time,labor pain,flooding postpartum bleeding, rate of cesarean section and rate of neonatal asphyxia were compared between two groops.Results　The pain score, labor time, rate of cesarean section, flooding, and rate of neonatal asphyxia of antalgic group was significant lower than that of control group(P<0.01).Conclusion　The combined spinal- epidural analgesia with ropivacaine and fentanyl is an effective method for labor analgesia, the method has the advantages of better antalgic effect, shorter labor duration, lower rate of cesarean section, less pestpartun bleeding,flooding, and lower rate of neonatal asphyxia etc.It is worthy of clinical application.]]> 2018/5/21 9:01:32 ZHOU Yong-er, LI Gen-xiang, PAN Zi-er, et al. 783true ［Abstract］　Objective　To investigate the association between SHBG-SNP Asp327Asn and recurrence of hepatocellular carcinoma (HCC) after radical resection.Methods　Materials of clinical pathology, blood DNA and prognosis survival of 131 HCC patients were collected. Univariate analysis and multivariate Cox regression analysis were used to detect the factors of recurrence of primary HCC after radical resection. The recurrence-free survival curves of genotypes of Asp327Asn were described by Kaplan-Meier analysis and the survival times were compared by Log-Rank test.Results　The overall survival rate and cumulative recurrence rate for 1, 2 years were 87%, 76% and 17%, 19% respectively. Both univariate analysis and multivariate analysis showed only number of nodules but not the polymorphism of Asp327Asn was related to the recurrence of HCC after radical resection. Survival analysis showed that, among HCC patients with a single nodule, although the recurrence-free survival time of Asp/Asn + Asn/Asn and Asp/Asp genotypes had no statistical difference (P=0.21), the later part of the survival curves separated obviously.Conclusion　Polymorphism of SHBG-SNP Asp327Asn may be not associated with recurrence of HCC after radical resection. However, polymorphism of the locus may have a certain influence on the long-term prognosis to those with a good liver basic condition.]]> 2018/5/28 8:15:14 LIU Shun, ZENG Xiao-yu, BAI Hua, et al. 782true ［Abstract］　Objective　To observe the changes of levels of IL-23 in serum and tongue tissues and the intervention effect of modified huopuxialing decoction in order to study the effect and significance of IL-23 in the pathogenesis of damp-heat syndrome. Methods　First,96 rats were randomly divided into normal group, damp-heat model group and medicine interference group.The damp-heat syndrome rat model was established by method of combining multi-factor. Medicine interference group was intervened by the modified huopuxialing decoction from the 25th day of modeling. Dosage was divided into two gavage and taken continuously for 7 days. At 4 h, 24 h, 48 h, 72 h after intervention, rats were sacrificed and sampled. The level of IL-23 in rat serum was detected by ELISA. The expression of IL-23 in rat tongue tissues was detected by SP-immunohistochemistry. Results　The levels of expression of IL-23 in serum and tongue tissues of model group were significantly higher than those in the normal group in each time point（P<0.01）. The levels of expression of IL-23 in model group were significantly highter than those in medicine interferention group in each time point（P<0.01）. At 4h, the levels of IL-23 in serum were the highest in model group, and decreased gradually with time point and the levels of IL-23 at 72 h and 48 h were significant differences in comparison with the levels of IL-23 at 4 h（P<0.05）,between levels of IL-23 at 72 h and that at 24 h there was significant differences（P<0.05）. In IL-23 expression levels in tongue tissues, there were statistically significant difference between these time points（P<0.01）. In mild, moderate and severe rat model with syndrome of damp-heat, the levels of expression of IL-23 in serum and tongue tissues showed a gradual increasing trend with the clinical symptoms. There were significant difference between these groups (P<0.01) and the content of IL-23 in serum and tongue tissues were positively correlated to the severity of syndrome of damp-heat significantly（P<0.01）. Conclusion　IL-23 maybe participated in the pathogenesis of syndrome of damp-heat and was significantly positively correlated to the severity of syndrome of damp-heat. Modified huopuxialing decoction can decrease the expression of IL-23 in rats with damp-heat syndrome, inhibit the inflammatory response and reduce the severity of the disease.]]> 2018/5/28 8:15:14 LI Shu-guang,LU Jun-ying,QUE Tie-sheng,et al. 781true ［Abstract］　Objective　To study the effects of low-dose hydrocortisone on hemodynamics and the concentration of BLA(blood lactic acid) in the patients with septic shock.Methods　Eighty patients with septic shock were divided randomly into the treatment group and the control group. Both groups were treated by normal medicine and the treatment group by low-dose hydrocortisone additional injection. HR,MAP,CI and BLA respectively before (baseline) and at 4,12,24,48 h and 7 d after the treatment were monitored. APACHEⅡ score were recorded before (baseline)and at 24, 48 h and 7 d after the treatment. Mortality rate was compared on 28th day.Results　The indexes of two groups were similar before treatment (P>0.05). In the treatment group,compared with control group, HR、MAP and CI at every time point were significantly improvement; compared with the baseline values before the treatment (P<0.05);the concentration of BLA at 4～12 h after the treatment significant decreased (P<0.05), and compared with the control group, the concentration of BLA at 4 h,12 h,24 h,7 d were decreased(P<0.05); the application time of catecholamine infusions in treatment group was less than that of control group.The 28d mortality rate in the treatment group was significantly lower than that in the control group(P<0.05). Conclusion　Low-dose hydrocortisone can improve the tissue perfusion and systemic oxygen metabolism in patients with septic shock,which can raise successful rescue rate.]]> 2018/5/28 8:15:14 LIU Feng-lin, XIONG Bin, LIN Yong-jun, et al. 780true ［Abstract］　Objective　To explore the function and the possible mechanism of silicone agents for hyperplastic scar, through silicone gel spray treating on a rabbit ear′s hyperplastic scar model.Methods　Eight New Zealand white rabbits were randomly divided into untreated control group and silicone gel spray group. Hyperplastic scar model was established on the ventral side of rabbit ears by operation. The administration of drugs was given from the 28th day after the operation. Animals were scarified and scar tissues were detached on the 56th day post operation, after 28 days of treatment interval. Detection of the scar tissue hyperplasia index (HI) and the numerical density of fibroblasts on area (NA) were performed under H&E staining; tissue collagen fiber area density (AA) was analyzed under VG staining. Immunohistochemistry was applied to exam expression of Smad3 in the tissues.Results　Compared with control group, the silicone gel spray group had scars with smaller fibroblasts; more regulatory and sparse collagen fibers; dramatically lower HI, NA and AA. In addition, expression of Smad3 level in the silicone gel group was significantly lower than control group by immunohistochemical staining.Conclusion　Silicone gel spray can lower the expression of Smad3, restrain fiber cell proliferation and collagen formation, and therefore inhibit the growth of scar in rabbit ear′s scar model.]]> 2018/5/28 8:15:14 CHEN Ling, NONG Xiao-lin, CHEN Xiang, et al. 779true ［Abstract］　Objective　To detect the expression of cancer testis antigen (CTA) MAGE-A3 protein in human nasopharyngeal cancer(NPC) patients as well as rhinitis patients and explore its feasibility in developing special vaccine, immunodiagnosis, and immunotherapy of NPC.Methods　Immunohistochemistry method was used to detect the expression of MAGE-A3 antigen in tissues of 51 NPC patients as well as 20 rhinitis patients.The results of expression and clinical parameters were analyzed with SPSS software.Results　The Expression of MAGE-A3 was observed in 33 out of 51 NPC patients and 2 out of 20 rhinitis patients; the differences was statically significant(P<0.01).The expression of MAGE-A3 in NPC was not correlated with gender, age, lymph node metastasis status, or TNM stages (P>0.05).Conclusion　MAGE-A3 antigen is expressed in NPC at a high frequency,which suggests that MAGE-A3 may be used as target antigen for immunodiagnosis and immunotherapy of NPC.]]> 2018/5/28 8:15:14 ZHOU An-xiu,LIAO Hong,LI Xiao-lin,et al. 778true ［Abstract］　Objective　To evaluate the achievement in the first stage of “Sight First-China Action” in Guangxi by investigating the training of oculists and comparing the ophthalmological condition of county hospitals before and after training.Methods　The research object were oculists of 54 cunty hospitals from 1998 to 2004. The trainee′s ophthalmological knowledge and clinical skills before and after training were compared. Furthermore, Ophthalmological condition in 54 county hospitals were investigated by questionnaire.Results　Two hundread and fifteen oculists had been trained in the project.The theoretical level of them had been improved obviously and much better than before（P<0.01）.All of the trainee could master the techniques of ECCE and IOL implantation.The number of oculists and hospitals with independent opthalmological department had increased;moreover,the program enabled a large increase in the number of patients,cataract operation and equipment.The rate of prevention blindness increased from 81.95% to 86.98%.The rate of prevention disability also increased from 63.09% to 76.79%.Conclusion　The training of oculists was fruitful.The medical quantity and equipment had been improved greatly,it also made local government realized the importance of prevention blindness.Taking everything into consideration,the project was a great success.]]> 2018/5/28 8:15:14 LI Li,YAO Gang,LIU Lu-hong,et al. 777true ［Abstract］　Objective　To study the rule of magnetic resonance imaging (MRI) in the diagnosisi of residual- relapse lymph node metastasis in patients with nasopharyngeal carcinoma after chemotherapy and/or radiation therapy.Methods　Forty-two patients proved residual or relapse and fourteen patients proved reactive by pre-operation histopathology were studied retrospectively. All patients had had their neck scanned by MRI with plain and contrast enhanced sequences.Results　Central necrosis and extracapsular spread and fusion were easier to be seen in residual- relapse lymph node in post-therapy nasopharyngeal carcinoma after therapy. the distribution was as follows: 2（4.6%） in level Ⅰ, 4（9.5%） in level Ⅱa,29 (69%) in level Ⅱb, 13 (30.9%) in level Ⅲ, 10 (23.8%) in level Ⅳ, 11 (26.2%) in level Ⅴ, 1（2.3%） in level Ⅵ, 1（2.3%） in level Ⅶ, 1（2.3%） in retropharynx, 1（2.3%） in Parotid.In these patients, a total of 178 postive node, including 108（60.7%） extracapsular spread nodes, were detected. The proportion of nodal extracapsular invasion was higher when the axial diameter increased (P=0.000).Conclusion　The level Ⅱb and Ⅲ were the most frequently involved regions. The proportion of extracapsular invasion of residual or relapse lymph node was positively correlated with axial diameter of lymph nodes. MRI is an important method in diagnosis of in residual- relapse lymph node in post-therapy nasopharyngeal carcinoma after therapy.]]> 2018/5/28 8:15:14 HE Cheng-cheng，SI Yong-feng，WEI You-yong, et al. 776true ［Abstract］　Objective　To explore the treatment method and effect of osteoporotic hip fracture in the elder.Methods　One hundred patients over 70 years old(age ranging from 70～96) with osteoporotic hip fracture were treated by surgery （92 patients） or no-operation 8 patients. There were 39 males in the series. Their mean age was 74.4 years. And there were 61 female, their mean age was 71.3 years. Out of 29 patients with femoral intertrochanteric fracture, 20 patients were treated by DHS internal fixation. Eight patients were treated by non-operative methods. One patient by replacement of artificial femoral head. Among 71 patients with femoral neck fracture, 33 patients were treated by replacement of artificial femoral head, and 38 patients by total hip arthroplasty. All patients were treated with antiosteoporotic drugs.Results　All patients were followed up for an average 14.2 months (ranged from 6 months to 28 months). After treatment, femoral head ischemia and necrosis were found in 4 patients. The internal fixation loosening or slipping in 5 patients. The break of internal fixation in 2 patients. Pain caused by artificial femoral head sinking in 5 patients. Eighty-four patients achieved satisfactory results.Conclusion　The treatment of osteoporotic hip fracture in the elderly can achieve satisfactory results if the comprehensive therapies are given on the basis of surgery.]]> 2018/5/28 8:15:14 CHENG Peng, LIU Wen-he, YAN Lin-lin,et al. 775true ［Abstract］　Objective　To investigate the expression of P-selectin and E-selectin in acute necrotizing pancreatitis (ANP) associated with liver injury in rats,and evaluate the effect of low molecular weight heparin(LMWH).Methods　Ninety-six Sprague-Dawley rats were randomly and equally divided into control group(group C,n=32),ANP group(group A,n=32) and LMWH intervention group(group G,n=32).In group A,the rats was induced retrograde injection of 1.5% sodium deoxycholate into the biliary-pancreatic duct to induce ANP(1 ml/kg).In group G,after modeling,subcutaneous low molecular weight heparin calcium (10 U/100 g).In group C,the abdomen was closed only after turning the pancreas.The rats were killed at 6 hours,12 hours,24 hours and 48 hours.The level of serum amylase was detected.The level of serum TNF-α and IL-6 was determined by ELISA.The pathologic changes of pancreas and liver were observed and graded under the microscope.The expression of P-selectin mRNA and E-selectin mRNA in liver were evaluated by RT-PCR.Results　The comparison of each observation index of the three groups and different time points reached statistical significance(P<0.01）,At each time point,the pathologic scores of the pancreas and liver,the level of serum-amylase and TNF-α、IL-6 in serum,the expression of P-selectin mRNA and E-selectin mRNA in liver of group A were significantly higher than group C (P<0.05),while the pathologic scores of the pancreas and liver,the level of serum-amylase and TNF-α、IL-6 in serum,the expression of P-selectin mRNA and E-selectin mRNA in group G were significantly lower than group A (P<0.05). Conclusion　The expression of P-selectin and E-selectin may concerned with the level of liver injury in acute necrotizing pancreatitis.LMWH may alleviate liver injury by its inhibition of the expression of P-selectin and E-selectin in acute necrotizing pancreatitis.]]> 2018/5/28 8:15:14 XU Zhi-yi,TANG Guo-du,ZHANG Yu-hong, et al. 774true ［Abstract］　Objective　To summarize and analyze the operation skills of transvaginal operation for tubal pregnancy.Methods　The surgical treatment conditions of 52 cases of tubal pregnancy undergoing transvaginal operation were retrospectively analyzed.The operation time, intraoperative blood loss, postoperative body temperature, white blood cellcount( WBC ),postoperative anal exhaust time, postoperative hospital stay, hospital costs, and so on were observed.Results　Fifty-two patients were operated successfully. The operative time was (44±13.4) min,intraopcroctive blood loss (34 ± 6.6)ml, anal exhaust time (14.2±3.4)h, maximum temperature (37.3 ± 0.3)℃, postoperative blood was (9.5±1.5)×10⁹/L, length of stay (4.5 ± 1.3)d, hospitalization costs (3502.8±356.8)yuan (RMB). The average time of serum HCG dropping to normal was (10.6±2.4)days, At 1 month after operation tubal patency rate was 81.8%(9/11).Conclusion　Under the premise of obtained therapentic effect transvaginal operation for tubal pregnancy has aolvantages of minimally invasion, quicker recovery, lower cost and so on.]]> 2018/5/28 8:15:14 HUANG Er-hong,SU Li-ming 773true ［Abstract］　Objective　To observe the changes of arrhythmia in elderly patients with cardiovascular disease in perioperative period of cataract surgery with dynamic electrocardiogram (DCG), providing the basis for the development of interventions.Methods　Sixty-six patients(72 eyes) with cardiovascular disease aged over 60 years undergoing cataract surgery were observed with DCG in the perioperative period.The DCG data at 2 h before operation,during operation,and at 2 h after operation were comparatively analyzed using statistical methods.Results　In the perioperative period, the incidence of arrhythmias was 87.9%, atrial arrhythmia 86.2%, ventricular arrhythmia followed by 72.4%.Short-term atrial tachycardia and transient ventricular tachycardia were 24.1% and 3.4% respectively.The incidence of the atrial arrhythmia was higher and the incidence of ventricular arrhythmia was lower at 2 h before operation compared with those during operation and at 2 h after operation (all P=0.000) In addition, the oculocardiac reflex occurred in 23 patients (34.8%) during surgery. The off-remnant rate was 86.1 % and The off-blindness rate 93.1% when patients were discharged from hospital. There were not serious systemic complications in surgery.Conclusion　The incidence of arrhythmia in elderly patients with cardiovascular disease in perioperative period of cataract surgery is higher.Strengthening the comprehensive analysis and evaluation before operation, taking measures to reduce the occurrence of arrhythmia in view of the change characteristics and minimizing surgery risk,which can improve the prognosis of cataract surgery in elderly patients with cardiovascular disease.]]> 2018/5/28 8:15:14 LIANG Jian-yi,SUN Zhong-bo, ZENG Si-ming,et al. 772true ［Abstract］　Objective　To investigate the effect of siRNA-mediated gene silencing on the expression of Rb gene protein in MGC803 cells.Methods　The siRNA expression plasmids against E2F-1 were transfected into MGC803 cells in vitro. After 48 hours, E2F-1 mRNA expression levels were detected by semi-quantitative RT-PCR and western blot analysis.The levels of total Rb protein and Rb phosphorylation protein expression were also analyzed by western blot. And the expression of the dephosphorylation of Rb could be calculated from these results above.Results　Expression of plasmid DNA was stable after transfection into MGC803 cells and the mRNA level of E2F-1 in MGC803 cells was downregulated significantly comparing with normal group and negative control group. Western-blot analysis has shown that the levels of the expression of E2F-1 protein was downregulated in MGC803 cells after interfered with siRNA (83.2%, 84.6%, P<0.05) while the expression of Rb dephosphorylation protein was upregulated (61.22%, 66.60%, P<0.05).Conclusion　The mRNA and protein level of E2F-1 can be downregulated by using RNA interference in MGC803 cells, and therefore siRNA-mediated E2F-1 upregulate the activity of Rb phosphorylation protein. There might be some antagonistic effects between E2F-1 gene and Rb gene. So E2F-1 might be used in clinical gene therapy for gastric carcinoma.]]> 2018/5/28 8:17:41 KONG Fan-biao, WANG Xiao-tong, WEI Wei-yuan, et al. 771true ［Abstract］　Objective　To develop a new real-time assay(R/P assay) for detection of Mycobacterium tuberculosis complex and evaluate the application value of R/P assay in detection of Mycobacterium tuberculosis complex (MTBC) in clinical specimens.Methods　The primers and probe were designed and constituted standard sample. The R/P assay was applied to 54 specimens of the clinical tuberculosis cases and the results were compared with those of culture and real-time PCR.Results　The positive rate of R/P assay,Real time PCR,culture by the different clinical specimens were 96.3%，83.3% and 55.6% respectively.Conclusion　The R/P assay is a rapid and specific method for direct detection of MTBC, which also can distinguish viable and nonviable MTBC, thus may guide patient therapy, and public health decisions.]]> 2018/5/28 8:17:41 JIANG Li-juan, WU Wen-juan, WU Hai, et al. 770true ［Abstract］　Objective　To explore the possibility of Guangxi brown spotted duck as hepatitis B virus infection animal model.Methods　One-day old Guangxi brown spotted ducks were infected with duck hepatitis B virus (DHBV), and DHBV strong positive ducks were detected by the real-time fluorescence quantitative PCR (FQ-PCR) at 13 day after the infection of DHBV.Results　One hundred and forty-eight serum samples in ducks were tested, and 136 samples (91.9%) were positive for DHBV.Conclusion　The Guangxi brown spotted duck may be used as hepatitis B virus infection animal model. FQ-PCR method for detection of DHBV is sensitive, repeatable and practicable.]]> 2018/5/28 8:17:41 CHEN Zhao-ni, HUANG Ren-bin, LUO Xiu, et al. 769true ［Abstract］　Objective　To study the effect of basic fibroblast growth factor (bFGF) on oxidative stress of myocardium in mice of coxsackievirus B₃ (CVB₃) induced viral myocarditis(VMC).Methods　Male Balb/c mice were inoculated intraperitoneally with CVB₃ to reconstruct an animal model of myocarditis. Seventy-five male Balb/c mice were randomly divided into five groups (n=15): normal control group, VMC group, bFGF low-dose, middle-dose, high-dose intervention groups. bFGF was administrated by intramuscular injection (im). The mice were inoculated intraperitoneally with CVB₃ while im bFGF (the dosage of bFGF in three intervention groups were 1 000.0,2 000.0,4 000.0 IU·kg^-1, respectively) each day one time for six times. All mice were sacrificed at the seventh day after inoculated intraperitoneally with CVB₃. The pathological changes of myocardial tissues were observed with light microscope. The contents of superoxide anion (O₂^.-), hydrogen peroxide (H₂O₂), hydroxyl radical (·OH) and malondialdehyde (MDA) were determined by spectrophotometry in myocardial tissues. The contents of metallothionein (MT) and cardiac troponin Ⅰ (cTnⅠ) were determined by enzyme-linked immunosorbent assay method in myocardial tissues and serum respectively. The expressions of myocardial MT-Ⅰ mRNA and MT-Ⅱ mRNA were detected by reverse transcription-real time fluorescent quantitative polymerase chain reaction method in myocardial tissues.Results　The contents of O₂^.-, H₂O₂, ·OH, MDA and pathological scores in myocardial tissues, the content of cTnⅠ in serum in VMC group were significantly higher than those in normal control group (P<0.01), the expressive levels of MT-Ⅰ mRNA, MT-Ⅱ mRNA and content of MT in myocardial tissues in VMC group were significantly lower than those in normal control group (P<0.01). The contents of O₂^.-, H₂O₂,·OH, MDA and pathological scores in myocardial tissues, the content of cTnⅠ in serum in bFGF three intervention groups were significantly lower than those in VMC group (P<0.01), the expressive levels of MT-Ⅰ mRNA, MT-Ⅱ mRNA and content of MT in myocardial tissues in bFGF three intervention groups were significantly higher than those in VMC group (P <0.01), the changes were obviously dose-dependent with bFGF in bFGF three intervention groups.Conclusion　bFGF inhibited the oxidative stress in myocardium induced by CVB₃, and thereby reduced injury of myocardium induced by CVB₃, this was related to bFGF antagonized the inhibitory effect of CVB₃ on the synthesis of MT in myocardium, thereby enhanced capabilities of scavenging of O₂^.-, H₂O₂,·OH in myocardium.]]> 2018/5/28 8:17:41 PAN Guang-ting, YANG Guan-ming 768true ［Abstract］　Objective　To establish the necessity and effectiveness of popularizing the norms on the diagnosis and treatment urinary calculi in the basic hospitals of Guangxi Zhuang Autonomous Region, and make the guideline of the diagnosis and treatment of urinary calculi which is suitable for the basic hospitals of Guangxi.Methods　Mashan was selected area as a pilot project, and the level of the diagnosis and treatment to urinary tract calculi in medical staffs was evaluated before performing the effective training. The differences between pre- and post- training were compared.Results　The investigation before training showed that the medical staffs of Mashan had poor knowledge of diagnosis and treatment to urinary tract calculi, especially who were in town and village hospitals. The awareness rate were 44.8% before training and 82.3% after training (χ²=170.03, P<0.01); The average score increased from (44.27±17.21)min before training to (80.07±5.77) after training (t=-19.52, P<0.01)).It showed favourable effects.Conclusion　The medical staffs in basic hospitals of Guangxi region lack knowledge of diagnosis and treatment to urinary tract calculi. The significant effects were evident through effective training, which was necessary for the medical staffs and was worth widely using in basic hospital of Guangxi.]]> 2018/5/28 8:17:41 LIU Gang, WANG Jian, WANG Xiao-ping, et al. 767true ［Abstract］　Objective　To study the anti-tumor effects of sodium citrate combined with arsenic trioxide on human gastric cancer cell line SGC-7901 in vitro and to explore its possible mechanism.Methods　The passaged and cultured gastric cancer cell line SGC-7901 in vitro were treated with sodium citrate ( final concentration was 5mmol/L，CT5) and(or)As₂O₃ (final concentration were 5 μmol/L，AS5).Cell proliferation was observed by fluorescent inversive microscopy; Cell apoptosis was explored by DAPI fluorescent stain, then the cell apoptosis rate and phases of cell cycle were determined by flow cytometry. The expression of apoptosis-related genes of bcl-2 was observed by RT-PCR.Results　Sodium citrate combined with As₂O₃ had a stronger inhibition effect on human gastric cancer cell line SGC-7901 than sodium citrate or As₂O₃(P<0.05). Compared with blank group,the cell cycle distribution was altered and the ration of G₀/G₁ phase cell decreased and G₂/M phase increased (P<0.05). After intervened by sodium citrate combined with As₂O₃,the expression of bcl-2 decreased（P<0.05）.Conclusion　Sodium citrate combined with As₂O₃ has the synergistic effects on gastric cancer cell line SGC-7901,and the mechanisms may be related to cell cycle blocking and apoptosis-inducing effects through regulating the expression of the related genes.]]> 2018/6/4 8:29:26 ZHENG Bu-ping, ZHANG Xiao-dong, LU Yun-fei 766true ［Abstract］　Objective　To investigate the related factor of ischemie renal disease in middle and elderly population, to provide the possibility for early diagnosis and detection.Methods　The research object included 3 000 employees and retirees (aged from 40～85 years) from the Physical Examination Center of our hospital from Jan to Dec in 2007.Their related data were colleted by using questionnaire and surveying medical examination information.(1)Investigated the awareness rate of the diseases, and analyzed incidence, constituent ratio on causation of disease, clinical characteristics and current therapy among them.(2)Analyzed the predicted-independent risk factors in the progression of ischemic nephropathy.Results　(1)The awareness rate of ischemie renal disease was low: patient 3.29%，crowd 0.70%.(2)With the development of age, prevalence rate (5.07%) increased obviously, and the differences in prevalence rate between male and female were not statistically significant.(3)Clinical characteristics:hypertension（83.56%）, renal hypofunction （63.16%）; main complication: heart disease（63.81%）,cerebrovascular disease（27.63%）, carotid atherosclerosis（55.92%）,diabetes（29.60%）,lipid dysbolism（53.29%）.Conclusion　The awareness rate of ischemie renal disease is low; with the development of age, prevalence rate increased obviously; the independent risk factors include: smoking, advanced age, severity of coronary artery disease, systolic hypertension, cerebrovascular disease, carotid atherosclerosis, diabetes, hyper-low density lipoprotein cholesterolemia.]]> 2018/6/4 8:29:26 WU Qiu-xia,TANG Sheng,ZENG Chun,et al. 765true ［Abstract］　Objective　To investigate the change of the hyoid bone position before and after extraction orthodontic treatment in adolescent patients with classⅠmalocclusions.Methods　Nineteen adolescent patients with classⅠcrowding or bimaxillary protrusion malocclusions who received orthodontic treatment by straight wire appliance and with 4 first premolar extraction were collected.The period of the correction was 22～27 months. Cephalometric analysis of each patient were performed beforre and after trearment on the vertical and horizontal position of hyoid bone.The measurements were tested by statistical analysis.Results　After extraction treatment, all the measwement of the horizontal and vertical position of hyoid bone were larger,and the change of most measurements of the horizontal and vertical position of hyoid bone showed significant difference (P<0.05).Conclusion　After two years of extraction orthodontic treatment, the hyoid bone position became more posterior and lower in adolescent patients with class Ⅰmalocclusion.]]> 2018/6/4 8:29:26 FANG Zhi-xin, ZHOU Yan, ZHOU Jing, et al. 764true ［Abstract］　Objective　To observe the effects of large does of pravastatin on the charnge of C reactive protein (CRP),Matrix metalloproteinase-9(MMP-9) in acute myocardial infarction patients.Methods　72 patients with acute myocardial infaction were divided into two groups: the group A were administered orally pravastatin 40 mg/d and the group B were administered orally pravastatin 20 mg/d. The serum levels of CRP, MMP-9 were measured at the end of the treatment.Results　The two groups serum levels of CRP、MMP-9 were significantly decreased at the end of the 3th day(P<0.05),and the group A was superior to group B.Conclusion　The inflammatory reaction could be inhibited in acute myocardial infarction patients treated with pravastatin.]]> 2018/6/4 8:29:26 CHEN Ying, DUAN Xiao-yan, ZHANG Shu-feng, et al. 763true ［Abstract］　Objective　To investigate the reasonable and feasible approach, in surgical treatment of unilateral thyroid nodules in primary hospital without conditions of pathological diagnosis.Methods　Two hundred and six patients with unilateral thyroid nodules were divided into the study group （n=104）and the control group （n=102）.The study group was treated by ipsilateral thyroid gland lobe plus unilateral isthmus resection for thyroid nodules, the control group by ipsilateral partial thyroid resection, subtotal removal, simple excavation for thyroid nodules.Results　The postoperative pathological diagnosis showed papillary thyroid carcinoma in 12 patients and no secondary surgical treatment were performed in the study group，pathological diagnosis showed papillary thyroid carcinoma in 10 patients, and then underwent secondary surgery in the control group. There was a statistical signiticant difference between groups (P<0.01).In study group brief recurrent laryngeal nerve paralysis was found in 1 patient, in the control group permanent recurrent laryngeal nerve paralysis was found in 2 patients. No postoperative respiratory difficulties and choking, laryngeal nerve damage, hypothyroidism, hypoparathyroidism, etc. were found in two groups.There was no statistical signiticant difference in post-operative complications between the two groups (P> 0.05).In study group 12 patients with papillary thyroid carcinoma were followed up, the patient survived.Conclusion　Ipsilateral thyroid glandhbe plus isthmus resection is a good and feasible surgical method for unilateral thyroid nodules in primary hospital without condition of pathological diagnosis.]]> 2018/6/4 8:29:26 FAN Xian-wei, HAN Xiang, WENG Jing,et al. 762true ［Abstract］　Objective　To investigate the mortality rate and the causes of death of in-patients with acquired immune deficiency syndrome (AIDS).Methods　A retrospective study was done on the clinical data of AIDS patients who died at the Fourth People′s Hospital of Nanning in 2010.Results　There were 137 AIDS in-patients died in 2010 in the hospital and the mortality rate was 10.56%, included 105 men and 32 female, the ratio of male to female was 3.28∶1. The median length of hospital stay was (12.82±16.21)days. 72.72% patients were with CD4⁺ T cell count <100/μl. The time from confirmation of HIV antibody positive to death in the patients accepted highly active anti-retroviral therapy(HAART) was significantly longer than that in the patients unaccepted HAART(Z=-5.042,P=0.000). The most common diseases caused death in these patients were pulmonary infection, infection of central nervous system and penicilliosis.Conclusion　The mortality rate of the AIDS patients in advanced stage was high. Varieties of the opportunistic infections were also the main causes of the death in AIDS patients. And HAART may prolong the survival time of AIDS patients.]]> 2018/6/4 8:29:26 LU Xiang-chan, HUANG Ai-chun, OU Ru-zhi, et al. 761true ［Abstract］　Objective　By modern medical experimental methods, at the level of biochemistry and molecular biology,to explore the mechanism of dahuanglingxian capsule in prevention and treatment of gallstones.Methods　Eighty health New Zealand White rabbits were selected and randomly divided into 4 groups: model groups, dahuanglingxian group, xiaoyanlidan group and normal control group 4 groups, with 20 rabbits in each group. For the model group, dahuanglingxian group and xiaoyanlidan group, they were made into the model of chronic liver injury. The dahuanglingxian group received dahuanglingxian capsules by gavage which were made into suspension, and the xiaoyanlidan group received xiaoyanlidan tablets by gavage which were made into suspension, while the control group were given saline water intragastrically. After 6 weeks observation, the samples were collected and the conditions of gallbladder stones were observed and compared. The related indexes in serum of rabbit were detected; the total RNA were detected in rabbit liver tissue, the gene expression relative amount were detected.Results　The dahuanglingxian group was better than the xiaoyanlidan group in decrease of cholesterol conent and increase of bile acid content.CYP7A1 and acbc11 gene expression in liver tissue in the model group were down-regulated significantly and were 54% and 49.9% of the normal control group′s; in dahuanglingxian group and xiaoyanlidan group CYP7A1mRNA and acbc11mRNA amount in rabbit liver tissue were lower than normal group,but were higher than model group,having a clear ascendant trend.At the same time, There were significant differences in therapeutic effect between the two groups.Effect of dahuanglingxian groups was better than that of xiaoyanlidan group(P<0.05),particularly in improving the expression of acbc11 gene.Conclusion　Dahuanglingxian capsules can repair damaged liver cells,stabilize the gene expression of CYP7A1 in liver cells and bile salt export pmps acbc11,improve the metabolism of cholesterol and bile acid in the liver, effectively protect the liver function, and play a role in the reduction and prevention of gallstone formation.]]> 2018/6/4 8:30:03 TANG Qian-li,HUANG Wei,HUANG Ming-wei,et al. 760true ［Abstract］　Objective　To investigate the correlation between the type of vessels and quantitative analysis vessels index (VI) detected by 3D-CPA and microvessel density (MVD) by pathological specimens after operation in ovarian tumour.Methods　The vessels index(VI) and the type of vessels were detected by 3D-CPA in 116 patients who had ovarian tumour. After operation the pathological specimens were examined by immunohistochemical staining to detect MVD by applicating single anti-CD34 clone antibodies.The relevant analysis were made to definite the correlation between VI and pathological MVD.Results　The VI and MVD of Ⅰ～Ⅳ type vessels increased gradually.The VI and MVD of Ⅲ and Ⅳ type vessels were significantly higher than those of Ⅰ～Ⅱ type vessels,and the VI and MVD in malignant tumour groups were significantly highter thanthose in benign tumour groups（P<0.05）.There was positive correlation between VI and MVD （r=0.83，P<0.01）.When the VI≥0.04 /cm³ served as diagnostic criteria of ovarian malignant tumour,sensitivity and specificity were 90.3%,82.2% respectively.Conclusion　The vessels index(VI) and the type of vessels detected by 3D-CPA were closely correlated with MVD. 3D-CPA can well reflect generating conditions of the ovarian tumour vessels.It was an effective way to identify benign or malignant of ovarian tumour.]]> 2018/6/4 8:30:03 ZHENG Hong-yu,SHEN Gui-xin,KANG Li-ke,et al. 759true ［Abstract］　Objective　To study the related factors influencing the degree of acute poisoning in region of Guangxi.Methods　The study was made by consulting medical records and using classification analysis.Results　In 6 011 cases,the numbers of light, medium, heavy and death were 2 577,2 038,1 396 and 157 respectively (in order 42.87%,33.90%,23.22%,2.61%).The analysis of all kinds of influencing factors and of the case distribution were made.In the region of a disease, the rural cases (61.84%) were the most,and the severe poisoning and mortality rates (25.88%, 3.38%) the highest.In the form of a disease,the sporadic cases (89.33%) were the most,and the severe poisoning and the death accounting for the number of total constitution respectively 96.85%, 95.54%.In the age group,the cases (68.84%)of the group in 20 to 60 years old were the most,and the severe poisoning and the death rates respectively 71.78%、72.61%.In the poisoning causes,and the case number (91.89%) of the accidental, suicide and mistake-eating was the most,and the severe poisoning and the death rates respectively 93.76%,92.99%.In the poison category,the number of cases (61.23%) of pesticides and chemical class was the most, and the numbers（60.24%,73.25%）of the severe poisoning and the death the most.Conclusion　The rural, sporadic, 20 to 60 years of age, accidental, suicide, mistake-eating, pesticide and chemical kind in the factors influencing the numbers of a disease and the poisoning degree were the most prominent.There should become the focus of prevention and control of acute poisoning in the region of Guangxi.]]> 2018/6/4 8:30:03 ZHANG Zhen-ming,JIANG Dong-fang,LIU Qing-hua 758true ［Abstract］　Objective　To evaluate the application of wide field inversion lens system for treatment of macular hole retinal detachment.Methods　Wide field inversion lens system with cryoretinopexy under microscope were applied in 28 cases (28 eyes) of macular hole retinal detachment. The rate of retinal reattachment, visual acuity and complication were analyzed.Results　The follow-up time were 6～24 months. The visual acuity was improved in 26 eyes and not changed in 2 eyes. The rate of success was 92.9%.Conclusion　Wide field inversion lens system can increase the rate of macular hole retinal reattachment.]]> 2018/6/4 8:30:03 LI Min, YANG Ke, ZHAO Xin, et al. 757true ［Abstract］　Objective　To explore the correlation between the serum levels of testosterone (T), estradio (E₂),noradrenaline (NE) and the heart function in aged male patients with chronic heart failure(CHF).Methods　The serum levels of T, E₂ and NE levels were determined by ELISA in 100 aged male patients with CHF and 100 ages matched healthy individuals.Results　（1）Serum T in CHF group was significantly lower than that in the control group; The T level in CHF patients with Ⅱ, Ⅲ, Ⅳ level of cardiac function was step down(P<0.01),NE level with severity heart failure was gradually increasing;(2) The serum E₂ level was no significantly correlated with cardiac function (P>0.05).Conclusion　The determination of serum T,NE level can be used for heart function evaluation of older male patients with heart failure.]]> 2018/6/4 8:30:03 LI Guo-feng,SUN Li-wei,Li Xiao-bing,et al. 756true ［Abstract］　Objective　To detect the special AT-rich sequence binding protein 1(SATB1) expression in oral benign and malignant tumors and analyze its relationship with pathological classifications of oral squamous cell carcinoma.Methods　The SATB1 expression was detected by immunohistochemical staining(SABC) in 36 cases of oral squamous cell carcinoma, 14 cases of adenoid cystic carcinoma, 4 cases of pleomorphic adenoma, 4 cases of leukoplakia and 2 cases of normal oral mucosa.Results　The expression of SATB1 was mainly in cytoplasm of 14 cases of squamous cell carcinoma(38.9%), and in nucleus of 14 cases of adenoid cystic carcinoma(100.0%). Weak positive expression in pleomorphic adenoma and leukoplakia were found in 1 case respectively. There was no obvious expression in normal oral mucosa. The positive rate of SATB1 in class Ⅱ～Ⅲ oral squamous cell carcinoma was obviously higher than that in class Ⅰ(P<0.01).Conclusion　The expression of SATB1 is mainly in cytoplasm of squamous cell carcinoma, and in nucleus of adenoid cystic carcinoma. SATB1 is correlated with pathological classifications of oral squamous cell carcinoma.]]> 2018/6/22 16:33:56 LUO Ting-ting, QING Hai-yun, WU Jia-xiao, et al. 755true ［Abstract］　Objective　To observe the efficacy of psychological support for the patients before the extraction of the mandibular mesial-orientation impacted wisdom teeth.Methods　One hundred patients with mandibular mesial-orientation impacted wisdom teeth were randomly divided into two groups. In the experimental group, 50 patients accepted psychological support before the extraction of the mandibular impacted wisdom teeth. And in the control group, 50 patients received the operation directly. The heart rate,blood pressure before and after the extraction, and cooperation degree of every patient were recorded.Results　There was a statistically significant difference in the change of heart rate,and blood pressure before and after the extraction,and the cooperation degree of the patients between two groups (P<0.01).Conclusion　It is necessary to provide psychological support for the patients before the extraction of the mandibular mesial-orientation impacted wisdom teeth.]]> 2018/6/22 16:33:56 GUO Hong-jian,LIANG Fei-zhao,LI Gang,et al. 754true ［Abstract］　Objective　To assess the advantages of the transcallosal transpellucidal interforniceal approach with terminal-ostomy in removal of tumors in the third ventricle and pineal region,and describe the microanatomy applied in this approach.Methods　The clinical data of 29 patients who underwent microsurgical removal of neoplasms in third ventricle and pineal region with transcallosal transpellucidal interforniceal approach and terminal-ostomy, between June 2008 and June 2011 were retrospectively analyzed.Results　Of 29 patients, total removal of tumors was achieved in 13 patients, subtotal removal in 7 patients; partial removal in 9 patients respectively. After removal of tumors, terminal-ostomy were performed in all 29 patients.Conclusion　Transcallosal transpellucidal inter-forniceal approach is commonly described as the preferred route for neoplasms involving the third ventricle and pineal region. The major advantages afforded by the transcallosal transpellucidal interforniceal approach include minimally disturbing the intracranial anatomy,the directly working corridor and morely removal of neoplasms. When the tumor cannot be entirely removed with this approach and germinoma verified by pathology result,the terminal-ostomy for a length between 5 and 7 mm, is executed to resolve hydrocephalus.]]> 2018/6/22 16:33:56 YE Jin,ZHONG Shu,XU Ke-bei, et al. 753true ［Abstract］　Objective　To evaluate the efficacy of the procedure for prolapse and hemorrhoids (PPH) in the treatment of rectocele.Methods　Sixty patients with rectocele were divided randomly into two groups. Thirty patients in the treatment group were treated by PPH and thirty patients in the control group by Sehapayah method.Results　The cure rate was 70.0% in PPH group and,that was 43.3% in Sehapayah group.There was significant difference between the two groups(P<0.05).The incidence of difficult emptying and perineal expanding sense in the treatment group were lower than those in the control group (P<0.05 or <0.01).Conclusion　Compared with conventional Sehapayah method for rectocele,PPH is a simple,safe method with fewer complications and shorter healing time in the treatment of rectocele.]]> 2018/6/22 16:33:56 LIANG Chong,LAO Jing-mao,HE Wen-hai 752true ［Abstract］　Objective　To explore the eficacy, technique and complication of endovascular embolization in intracranial aneruysms with Matrix coils.Methods　Eighty aneruysms of 72 patients with intracranial aneruysms had been treated by Matix electrolytic coils embolization.Results　Tight embolization was found in 47(58.8%) aneurysms, residual neck in 24(30.0%), and tumor-like residues in 9(11.2%). Surgery-related complications occurred in 7 patients (9.7%), 2 patients died. Sixty-four patients following up for 6 to 48 months had no rebleeding or new neruological dedficit. Thirty-eight patients with 42 aneurysms received angiography from 6 to 24 months after treatment. Results showed stabilization in 26 aneruysms(61.9%), needed for further embolization in 5(11.9%), re-growth in 11(26.2%), recurrence and needed for re-treated in 2(4.8%) tumor-like residues.Conclusion　Matrix electrolytic coils embolization is safe, reliable, effective means of the treatment of intracranial aneruysms, and regular follow-up antiographic evaluation of embolization effects is necessary.]]> 2018/4/8 15:34:47 XIAO Quan, PANG Gang, LIU Ruo-ping, et al. 751true ［Abstract］　Objective　To study the protective effect of Ruanjian hugan tablet(RJHGT) on acute hepatic injury induced by carbon tetrachloride(CCl₄) in mice and its mechanism.Methods　CCl₄ was given by intraperitoneal injection to establish acute hepatic injury model. Thereafter, blood was detected the activities of ALT, AST, AKP and T-AOC in serum; Liver homogenate was measured the content of MDA, GSH and the activities of SOD, GSH-PX.Results　RJHGT can lessened the degree of hepatic injury. Compared with the model group, the activities of AST, ALT,AKP and the content of MDA in the treatment groups were decreased; while the ability of T-AOC, the content of GSH and the activity of SOD and GSH-PX were increased(P<0.0l or P<0.05).Conclusion　RJHGT has protective effect on acute liver injury induced chemically. The mechanism may be related to inhibiting the effect on lipid peroxidation.]]> 2018/4/8 15:34:47 MO Feng-zhen, KONG Xiao-long,QIN Zhen-ming, et al. 750true ［Abstract］　Objective　To establish radioresistant cell subline from a human nasopharyngeal carcinoma cell line CNE-3 and investigate the mechanism of radioresistance.Methods　Radioresistant cell subline were established from a human nasopharyngeal carcinoma cell line CNE-3 by repeating X-ray irradiation as radiotherapy.The radiosensitivity of this subline,together with its parent CNE-3 cell line,were measured.Results　This subline,named CNE-3R could be subcultured steadily three months,and kept SF₂>0.5.Conclusion　Radioresistant cell subline from a human nasopharyngeal carcinoma cell line successfully was established by using the method of research,which providing the tools for the study of the mechanism of radioresistance.]]> 2018/4/8 15:34:47 PENG Lu-xing, CHEN Jia-xin, CHENG Jin-jian, et al. 749true ［Abstract］　Objective　To compare p53 expression in oral squamous cell carcinoma of human and mouse.Methods　Immunohistochemical method was used to detect the expression of p53 gene in 8 cases of normal oral mucosa, 8 cases of oral dysplasia and 8 cases of oral squamous cell carcinoma (OSCC) in human and mouse, respectively. Results　In human normal oral mucosa, dysplasia, and OSCC, the positive p53 protein expression rates were 0.0%, 37.5 %, 62.5 %, respectively; while in mouse were 0.0%, 50.0%, 75.0%, respectively.Conclusion　There is the similar expression of p53 protein between human and mouse in same pathological stages. The development of the oral squamous cell carcinoma of the human and mouse may be similar to each other.]]> 2018/4/8 15:34:47 BI Si-chang,YU Da-hai,LI Ming-wu,et al. 748true ［Abstract］　Objective　To evaluate the curative effect of perceptual learning based on internet for juvenile anisometropic amblyopia,analyze the change of visual acuity and provide the reference for quickly and effective treatment of amblyopia children.Methods　Two hundred and sixteen juvenile patients (216 eyes) with anisometropic amblyopia were chosen and treated with the perceptual learning based on internet and the traditional combined training.The specific training programs were designed by doctor,which according to the vision initial state,degree of severity in low vision and advancement during training process.The perceptual learning group received the visual trainings,such as improving visual noise,contour integration and position noise; The traditional combined training group received red lights flashing and fine visual training.The total therapeutic effect of two groups were compared.Results　The total therapeutic effect of perceptual learning group was better than that of traditional combined training group（P=0.000）,the total therapeutic effect of patients with different level anisometropic in perceptual learning group was also better than that in traditional combined training group,there were significant differences (all P<0.01).The visual acuity of perceptual learning group was improved largely than traditional training group. The time when 50% patients have arrived curative plateau were different, perceptual learning group was 6m, however traditional group at the last follow-up.The cases in perceptual learning group needed 13.75 training hours to improve the visual acuity of one row sight, in which the shortest (10.78 hours) was the midrange amblyopia,then the severe amblyopia(12.00 hours) and the slight amblyopia (26.5 hours) in turn.Conclusion　The perceptual learning based on internet can provide intensive,active,feedback and individual visual stimulus,effective for juvenile aged 9～13 anisometropic amblyopia who out of vision sensitive developmental period,and shorten the treatment cycle of vision dysfunction.The new treatment can achieve the best treatment effectiveness in a short time and provide a new feasible way for amblyopia clinical therapy.]]> 2018/4/8 15:34:47 LIN Quan, LIU Wei-Min, XIAO Xin,et al. 747true ［Abstract］　Objective　To compare the efficacy between the modified anterolateral approach and the traditional lateral approach in total hip arthroplasty to provide reference for clinical selection of surgical approaches.Methods　From August 2006 to May 2011,161 hips of 152 patients received total hip arthroplasty were selected and randomly divided into two groups,including 79 hips of 73 patients treated by the modified anterolateral approach and other 82 hips of 79 patients by the traditional lateral approach.The follow-up time lasted from 15 months to 5 years,with an average of 4.3 years.The patient′s age, sex, diagnosis, height, weight, body mass index, incision length, blood loss, operative time, postoperative drainage, the time off bed for walking, hospital stay, Harris score before and after surgery, postoperative complications etc.were recorded, and analysised by SPSS 16.0 between the two groups.The numeration data were tested with chi square test.The measurement data are tested with test.Results　In the modified anterolateral approach group, in the operative time, the incision length, blood loss, postoperative drainage, length of stay, the time off bed for walking were significantly lower than those in the traditional incision group（P<0.05）.There were no significant difference postoperative Harris hip score,fracture & deep vein thrombosis, prosthetic loosening and other complications（P>0.05）.Conclusion　The modified anterolateral approach for the total hip arthroplasty reduced intraoperative and postoperative blood loss, shorterned operative time and hospital stay, lessened the risk of postoperative complications early recovered joint functional exercise. As well as it reduced the risk of surgical implant dislocation due to the improper location and the patients′ financial burden. So in the case of the same indications, prior consideration should be given to the choice of anterolateral approach.]]> 2018/4/8 15:34:47 CAI Min, LI Hong-yu, YIN Dong, et al. 746true ［Abstract］　Objective　To explore the change of the serum thyroid hormones level in the patients with acute myocardial infarction before and after treatment.Methods　In 82 patients with acute myocardial infarction,the serum thyroid hormones level were determined by ACCESS chemiluminescence apparatus before and after the treatment,and the results were compared and analyzed.Results　In the patients with the acute myocardial infarction,T₃,FT₃,TSH level was low before the treatment and significantly increased after the treatment,there was a remarkable difference before and after the treatment（all P<0.01）.Conclusion　The serum thyroid hormones level is relative to the severity of the disease,and the determination of serum thyroid hormones level has certain clinic value for the evaluation of treatment and prognosis of AMI.]]> 2018/4/8 15:34:47 MO Si-jian,LI Yan-mei,LIANG Tai-ying,et al. 745true ［Abstract］　Objective　To explore a set of scientific methods of perioperative antibacterial administration which are suitable for our hospital.Methods　Selecting the operation of mammary gland,thyroid gland and hernia as the research objects,publicizing the Guiding principles for clinical application of antibacterial to the surgeons and patients and intervening in antibacterials perioperative application,then the conditions of antibacterials application before,during and after intervention were compared.Results　The kinds of antibacterials after operation were more rational than before. The time of administration were shortened. The antibacterials costs of pure discectomy patients were lower than before, while the ratio of postoperative infection did not raise.Conclusion　Administrative intervention is the most effective intervention.]]> 2018/4/8 15:34:47 XIE Ling,CHEN Ying,ZHANG San-ping,et al. 744true ［Abstract］　Objective　To investigate the expression of thymidylate synthase (TS), glutathione S-transferase enzyme π(GST-π) and topoisomerase Ⅱ (Topo Ⅱ) protein before and after the recombinant human p53 adenovirus (rAd-p53) intratumoral injection and its significance.Methods　Eighty patients with advanced nasopharyngeal carcinoma were randomly divided into 3 groups：the gene therapy group (30 cases): rAd-p53 intratumoral injection and concurrent chemo-radiotherapy, the conventional chemoradiotherapy group (30 cases) and the radiotherapy group (20 cases). Clinical data and the nasopharyngeal tumor tissues which were intratumoral injection with rAd-p53 were collected. The two-step immunohistochemical was done to detect the TS, GST- π and TopoⅡ protein expression in the primary tumor tissue of nasopharyngeal carcinoma.Results　The expression TS, GST-π and Topo Ⅱ in conventional chemoradiotherapy group was significantly higher than that before treatment (P values were 0.01,0.01,0.00). In gene treatment group, the expression TS, GST-π and Topo Ⅱdid not change significantly (P>0.05).There were no significant difference in the expression of TS, GST-π and Topo Ⅱbefore and after radiotherapy alone(P>0.05). Among the three groups, the recurrence rate and metastasis rate were not significantly different (P = 0.95). The recurrence rate in GST-π negative patients was significantly lower than that in positive patients (8.93% vs. 54.17%, P = 0.00).Conclusion　GST-π protein expression is positively correlated with recurrence and metastasis in patients with advanced nasopharyngeal carcinoma after treatment.The rAd-p53 intratumoral injection can reverse chemo-resistance of nasopharyngeal carcinoma by inhibition of GST-π, Topo Ⅱ protein, which might be its mechanism of NPC treatment.]]> 2018/4/8 15:34:47 ZHANG Zheng, WENG Jing-jin, WEI Hai-ming, et al. 743true ［Abstract］　Objective　To explore the clinical significance of detection of heart rate variability(HRV) and gastric emptying in nasopharyngeal carcinoma(NPC) patients after radiotherapy.Methods　24-hour holter recordings were detected for analysis of time domain and frequency domain index of HRV and the evaluation of the gastric emptying was performed using barium stick in 30 NPC patients in before-radiotherapy,mid-radiotherapy and after-radiotherapy.Results　There were all results of HRV in 30 NPC cases after radiotherapy as followed:(1)There was statistical significance in the time domain index(SDNN,rMSSD,pNN50) comparison of three stages radiotherapy（P<0.01）.Compared with the before-radiotherapy and mid-radiotherapy,SDNN was lower in after-radiotherapy (P<0.01 or P<0.05); rMSSD and pNN50 in after-radiotherapy was lower than in before-radiotherapy （P<0.01）;(2)There was statistical significance in the frequency domain index (LF,HF,TP) comparison of three stages radiotherapy（P<0.05）.Compared with the before-radiotherapy and mid-radiotherapy,LF was lower in after-radiotherapy (P<0.05); HF and TP in after-radiotherapy was lower than in before-radiotherapy （P<0.05）.(3)There was statistical significance in 5-hour gastric emptying rate comparison of three stages radiotherapy（P<0.05）;5-hour gastric emptying rate in after-radiotherapy was lower than in before-radiotherapy （P<0.01）.Conclusion　HRV of NPC patients has become significantly decreasing with increasing of radiation doses.It indicates that the activity of vagus nerves is inhibited after radiotherapy in NPC patients,and has effect on sympathetic-vagal balance and autonomic nerves regulation.This leads to the decreasing of the gastric emptying.]]> 2018/4/11 8:05:21 CAI Lian-ying, ZHANG Qin, YANG Su-ping, et al. 742true ［Abstract］　Objective　To develop an antibody gene chip to detect specific protein expression profile of nasopharyngeal carcinoma(NPC) tissues.Methods　The specific antibody library containing anti-NPC antibodies were selected by using phage anti- body library. The V-D-J fragments as mark DNA molecules were used for preparation of antibody gene chip. After a number of different antibodies of specific antibody library binding the NPC tissues, the mark DNA molecules from the antibodies were amplified by PCR and the results of hybridization between the antibody gene chip and mark DNA molecules revealed protein expression profile of NPC tissues. The expression of key protein in situ of NP cells could be revealed by immunohisto- chemistry.Results　The concentration degree of combinative antibodies could be up to 10⁶～10⁸. The mark DNA molecule titer could reach about 10^-6～10^-8；Gene chip detected 10～14 positive antibodies in 20 protein expression profile. The positions of binding antibodies in cells were showed by immunohistochemistry.Conclusion　The antibody gene chip could detect protein expression profile in biopsies of NPC and could be widely used in basic research and clinical diagnosis and treatment process.]]> 2018/4/11 8:05:21 ZHU You-kai,SI Yong-feng,XIAO Rui-ping,et al. 741true ［Abstract］　Objective　To investigate the effect of closed lateral ventricle and lunbar cistern alternant circulation drainage combined with cerebrospinal fluid replacement for severe cerebroventricular haemorrhage (CVH).Methods　One hundred and forty-five patients with severe CVH were randomly divided into two groups:treatment group (n=71, treated by closed lateral ventricle and lunbar cistern alternant circulation drainage combined with cerebrospinal fluid replacement),control group (n=74, treated by traditional lateral ventricle drainage combined with lumbar centesis).The observation indexes included National Institutes of Health Stroke Scale(NIHSS), activities of daily living (ADL), Glasgow Coma Scale(GCS),Subarachnoid blood clearance time,days in hospital, mortality rate and complication.Results　After the treatment, NIHSS、ADL and GCS in the treatment group were （7.09±5.53）,（85.82±15.53）,（13.18±1.17）,and those in the control group were （15.91±7.26）,（53.86±13.39）,（10.05±1.46） respectively.There were significant differences in them between both groups(P<0.01). In the treatment group, incidence of cerebral vasospasm and hydrocephalus was 36.6%(26/71),and 11 cases(15.5%) died，which was lower than that of the control group ［59.46%(44/74) and 24 cases (32.4%)］. There were also significant differences in them between two group(P<0.05). The subarachnoid blood clearance time (5±1.9)d and days in hospital （23±4.7）d in treatment group were lower than those in the control group［(14±2.5 d),( 35±5.8 d)］.There were significant differences in them between the groups （P<0.05）.Conclusion　Closed lateral ventricle and lunbar cistern alternant circulation drainage combined with cerebrospinal fluid replacement for severe CVH can distinctly reduce mortality and disability rate，complication and days in hospital，and improve patients′ living ability.]]> 2018/4/11 8:05:21 LUO Ri-xiang,LI Ci-fa,WEN De-shu,et al. 740true ［Abstract］　Objective　To investigate the changes of ciprofloxacin concentration in bone and muscle tissues of the extremity of the rabbit after tourniquet inflation.Methods　The blood circulation of the extremity of the rabbit was blocked by tourniquet inflation at different times, separately 30 minutes, 60 minutes, 90 minutes or 120 minutes after administration of ciprofloxacin. Then the ciprofloxacin concentration in bone and muscle tissues was measured separately 30 minutes, 60 minutes, or 90 minutes after tourniquet inflation.Results　The changes of the concentration of ciprofloxacin were significantly different between the experimental group and the control group (P<0.05). In the experimental groups, those changes were significantly different when the timing of tourniquet inflation was different (P<0.05). However, the differences were not significant in the situation of the same timing but different duration (P>0.05).Conclusion　Blocking the blood circulation of the extremity of the rabbit with tourniquet can significantly influence the distribution of ciprofloxacin in bone and muscle tissues. However, the influence is not significant once the blood circulation has been blocked. The timing of blocking the blood circulation is the main factor that influences the distribution of ciprofloxacin in bone and muscle tissues.]]> 2018/4/11 8:05:21 SUN Ke, YE Dong-mei,LIANG Bin, et al. 739true ［Abstract］　Objective　To explore the recycle way and clinic application way of ACCESS chemiluminescence apparatus′ remainder reagent.Methods　ACCESS chemiluminescence apparatus′ reagent boxes were collected and saved. The recycle reagent was reunited to a box after collecting some amount. The reagent bar code was deciphered and the recycle reagent was read into the apparatus to use. The recycle reagent was used to test the serum and compared with the new reagent. Results　Within the period of validity,the test result used recycle reagent of same batch code was not remarkable different compared to the test result used new reagent（P>0.05）,but the test result used recycle reagent of different batch codes was remarkable different compared to the test result used new reagent （P<0.01). Conclusion　ACCESS chemiluminescence apparatus′ remainder reagent must be stored and recycled correctly. Within the period of validity,the test result used recycle reagent of same batch code is not different compared to the test result used new reagent,and the recycle reagent of same batch codes can be used to test clinic samples.]]> 2018/4/11 8:05:21 LI Yan-mei,MO Si-jian,LIANG Tai-ying,et al. 738true ［Abstract］　Objective　To investigate the intracranial pressure dynamic changes of patients with hydrocephalus, revealing the physiological characteristics of CSF circulation dynamics change of hydrocephalus, to provide accurate and simple indicator for shunt selection.Methods　With a non-invasive intracranial pressure monitor, intracranial pressure change was continuously monitored in patients with communicating hydrocephalus, drawing a pressure/time curve. In control group, preoperation lumbar cyst pressue were used as base line of intracranial pressure, and in experimental group, critical pressure point of the plateau of the pressure/time curve were used as base line. Selecting of the appropriate pressure of shunt system was based on the base line. The Salmon hydrocephalus shunt evaluation standard was used for assessment of patients′ prognosis of two groups.Results　There were no statistically difference in therapeutic effect at each time point between two groups, but there was statistically significant difference in improvement rate at six month between two groups (P<0.05).Conclusion　The critical pressure point of the plateau of the pressure/time curve can more accurately determine the ideal intracranial pressure value and have significant and wide application prospect in the guidance of the clinical treatment of hydrocephalus.]]> 2018/4/11 8:05:21 ZHONG Shu, LIANG You-ming, XIAO Quan, et al. 737true ［Abstract］　Objective　To evaluate the protective effect of taurine on renal injury induced by extracorporeal shock wave lithotripsy(ESWL).Methods　Sixty patients with kidney stones were divided into the taurine group(n=30) and the control group (n=30) at random. The two groups were respectively given 6g taurine and 6g placebo each day from the day before the ESWL to 6th day after the ESWL. The urinary samples were taken on the preoperative day and 1th,3th,7th day after the ESWL for determination of albumin (ALB),β₂-microglobulin(β₂-MG), endothelin (ET-1) and malondialdehyde (MDA).Results　There were no significant difference between the two groups of urinary ALB, β₂-MG, ET-1, MDA before the ESWL（P>0.05）. In the control group,the indexs were significantly elevated on the 1th,3th day after the ESWL compared with pre-treatment and the taurine group（P <0.001）.Conclusion　Taurine is effective in the protection of kidney against injury induced by ESWL.The mechanism may be related with its antioxidation capability and inhibition of ET-1 generation in kidney.]]> 2018/4/11 8:05:21 LAN Zhi-xiang, LIU Gang,FU Qiang,et al. 736true ［Abstract］　Objective　To compare the effect of two different conservative treatments to intervene primary osteoporotic thoracolumbar vertebral fractures,in order to search for the best scheme of non-surgical treatments of primary osteoporotic thoracolumbar vertebral fractures that combined Chinese medicine with Western medicine.Methods　Ninety cases of primary osteoporotic thoracolumbar fractures were divided into two groups,one was 30 cases for observation group and another was 60 cases for control group. Two different kinds of non-surgical treatments were developed by selecting appropriate treatments from both traditional Chinese medicine such as Jiawei Dajiang Zhuyu Prescription,Guben Shugan Zhuanggu Prescription,external medicine(Ironing), and western medicines including caltrate-D tablets,alfacalcidol soft capsules,Salcatonin Injection. Then the two different kinds of non-surgical treatments were applied to the two groups,and the efficacy,the quality of life and cost-effectiveness of the two groups were evaluated.Results　There was no significant difference for the clinical effect between the observation group and control group（P>0.05）; the total score for quality of life indicators was of no significant difference（P>0.05）,after treatment the effective scores of mental health and vitality in the two groups were higher than that before treatment（P<0.01）and the effective scores of mental health and vitality in the observation group was higher than that in the control group（P<0.01).The cost-effectiveness was better than that in control group（P<0.01).Conclusion　For primary osteoporotic thoracolumbar vertebral fractures patients,good curative effect can be gained when they are treated with integrative medical therapy of non-surgical at hospital combined with rehabilitation at home and when treated with full hospitalization. However,the former may bring the patients a better feeling,more stable emotion,more vigor,and a happier life.and the advantage of the former outweigh the latter in terms of health economics and should be recommended for a wider use.]]> 2018/4/11 8:05:21 XU Jian-wen,ZHONG Yuan-ming,ZHANG Jia-li,et al. 735true ［Abstract］　Objective　To analyze the correlation of neck circumference(NC)and obstructive sleep apnea hypopnea syndrome(OSAHS), to research the value of NC in diagnosis of OSAHS and find the best diagnosis score.Methods　From June 2009 to June 2012, a set of adult patients with snoring, arousal induced by breathing domination, or patients with hypertension and diabetes mellitus who intend to know the sleep status, were selected from the Sleep Disordered Breathing Center of the People′s Hospital of Guangxi Zhuang Autonomous Region. The age range of the sample was more than 18 years. The polysomnography(PSG) were undertaken in all patients. Before the PSG the height, weight, neck circumference, waist, hip circumference and so on were measured. The relationship of NC and OSAHS-AHI was analyzed, the ROC curve was used to research the value of NC in diagnosis of OSAHS, from which choosing NC score that had the best sensitivity and specificity.Results　Two thousand, one hundred and sixty-seven subjects meeting the research requirements had the complete research date. The mean age of them was (45.0±14.34)years, the age range was from 18 to 89 years, the mean NC was (38.17±3.567)cm. The female NC was significantly lower than the male ［（39.13±3.029）cm vs (34.07±2.683)cm, P=0.000］. Mild, moderate, and severe OSAHS patients′ NC were different ［（37.86±3.474)cm vs (38.24±2.935)cm vs (39.87±3.043)cm, P=0.000］. AHI and NC had correlation (r=0.109,P=0.000).PSG-AHI was used as The Gold Standard for making the ROC curve, when AHI≥15 was used as The Gold Standard the acreage under the ROC curve was maximum. When male NC ≥39.25 cm, sensitivity was 50.0%,specificity was 52.5%;female NC ≥34.75 cm, sensitivity was 75.5%,specificity was 58.6%.Conclusion　AHI and NC has correlation, to a certain extent, NC can be used to diagnose OSAHS, when male NC ≥39.25 cm,sensitivity was 50.0%,specificity was 52.5%;female NC ≥34.75 cm, sensitivity was 75.5%,specificity was 58.6%,it can to predict moderate or severe OSAHS.]]> 2018/4/11 8:05:21 SONG Wei,JIANG Li-jun,LIU Jian-hong,et al. 734true ［Abstract］　Objective　To investigate the clinical value of prostate specific antigen (PSA) in early screening prostate cancer.Methods　PSA were monitored in normal control group (30 patients),chronic prostatitis Ⅲ group (30 patients),benign prostatic hyperplasia group (30 patients) before prostate massage,within 1 h after prostate massage, 24 h after prostate massage.Results　PSA of normal control group，chronic prostatitis Ⅲ group,benign prostatic hyperplasia group was compared by self control test method.There were no significant difference between before and within 1 h after prostate massage(P>0.05);There were significant difference (P<0.01) among three time points(before prostate massage,within 1 h after prostate massage and 24 h after prostate massage).Conclusion　Within 1 h after prostate massage, PSA was not influenced, PSA monitoring might be of important clinical value for screening early prostate cancer to outpatients.]]> 2018/4/27 8:23:45 BI Ge-wen,ZHOU Yuan-ming,QING Zhi-biao,et al. 733true ［Abstract］　Objective　To study on the clinical meaning of mitral valve replacement(MVR) in beating heart through parasternal minimal invasive incision by partly cardiopulmonary bypass.Methods　From October 2007 to March 2012，80 patients with mitral valve replacement were divided into study group (A group, n=40) and control group (B group, n=40). A group was by parasternal minimal invasive incision(6～8 cm) and cardiopulmonary bypass establishment through right femoral artery and vein cannulation;B group was by right lateral chest routhly incision(12～18 cm) and cardiopulmonary bypass through aortic, superior vena cava and inferior vena cava cannulation. Both group underwent MVR in beating-heart.Results　Incision length: A group was （6.7±0.61）cm, B group was （16.51±1.52）cm(P<0.05); Operation time:A group was （74±12.00）min,B group was （71±11.00）min(P>0.05);Bypass time:A goup was （58±13.30）min, B group was （56±11.60）min (P>0.05); Bleeding:A group was （183±56）ml,B group was （196±43）ml(P>0.05);ICU time:A group was （36±8.5）h,B group was （37±7.8）h(P>0.05); Postoperative hospital-stay time: A group was （12±2.3）d, B group was （13±2.9）d(P>0.05).There were no incision infection,gas embolism, neurological complication.And there wre no vessel damage about aortic, femoral artery and vein cannulation.Conclusion　Mitral valve replacement(NVR) in beating heart through parasternal minimal invasive incision by partly cardiopulmonary bypass can reduce incision length, minimize the surgical injury,relieve pain. It can bring fast recovery and attractive looks. And it provide a minimally-invasive technique and can be popularized easily.]]> 2018/4/27 8:23:45 LIN Hui, WEN Zhao-ke, LI Xiang-wei, et al. 732true ［Abstract］　Objective　To analyze the death situation of pregnant and parturient women with HIV-infection in Guangxi, in order to offer evidence to reduce the mortality rate of such people.Methods　The data of maternal death was collected and analyzed by looking up maternal deaths report, attachments of maternal deaths, summary of maternal death records, report of HIV-infected pregnant women, follow-up cards, test results from laboratory and other ways.Results　Between 2007 to 2011,the maternal mortality rate in pregnant and parturient women with HIV-infection was 483.09/100 000,which was 22.96 times as that of the maternal mortality rate in Guangxi in the same period. The mainly causes of death in pregnant and parturient women with HIV infection was opportunistic infection, most of which were pulmonary infection. The social factors of death in pregnant and parturient women with HIV-infection were low cultural level, poverty and low health care respectively.Conclusion　Strengthening HIV screening to married women, putting pregnant women with HIV-infection into high risk pregnancy management scope and fully fulfilling《National implementation programme for preventing mother-to-child transmission》 will be effective ways to reduce the maternal mortality rate of pregnant and parturient women with HIV-infection.]]> 2018/4/27 8:23:45 MO Yun,HUANG Yue-hua,LI Ying 731true ［Abstract］　Objective　To discuss the effect of different surgical methods and time on the clinical efficacy of hypertensive intracerebral hemorrhage.Methods　Retrospective analysis of the clinical data of 278 cases of hypertensive intracerebaral hemorrhage were performed; hematoma puncture aspiration, minimally-invasive craniotomy or craniectomy & decompressive surgery were respectively chosen according to the patient′s state of consciousness, papillary changes, bleeding time, the site of bleeding and hematoma volume. The treatment of different surgery approach and its indication was discussed, and the effect given by the time of surgery was observed.Results　Eighty-one cases were treated with puncture aspiration, of them 58 cases had a good outcome in ADL, 21 cases were poor and 2 cases were dead(2.5%). One hundred and thirty-four case chose minimally-invasive craniotomy, of them 79 good in ADL, 42 cases poor and 13 were dead(9.7%). Sixty-three cases were treated with craniectomy & decompressive surgery，of them 23 good, 28 poor and 12 dead(19.0%). The 134 cases with minimally-invasive craniotomy were further observed，51 of them accepted surgery in less than 6 hours after haemorrhage, of them 37 had good outcome after 6 months of the surgery, 11 poor, 3 dead. In addition, 37 cases accepted the same surgery after 6 hours but less than 24 hours, of them 19 good, 14 poor and 4 dead. Besides, in the rest 46 cases who accepted surgery after 24 hours, of them 23 good, 17 poor, 6 were dead.Conclusion　Choosing optimal surgical approach based on the evaluation of the intracerebral hemorrhage with multiple data and giving surgery in time can improve the effect and reduce the mortality.]]> 2018/4/27 8:23:45 XIAO Quan, LIANG You-ming, CHEN Hai-jun, et al. 730true ［Abstract］　Objective　To study the correlation among prehepatic fat thickness and visceral fat thickness by ultrasonography and mearurements about obesity in the young and middle-aged female.Methods　Detected subcostal prehepatic fat thickness and visceral fat thickness of 1 centimetre above the umbilicus by ultrasound in 330 females aged from 18～47 years, and mearued the heigh,weigth,the circle of waist, the circle of hip et al, and the results was compared.Results　There is a positive correlation between the prehepatic fat thickness and visceral fat thickness, and also in weigth, BMI, the circle of waist, the circle of hip et al.Conclusion　Prehepatic fat thickness and visceral fat thickness has a good correlation by ultrasonic tested, and it also has a good correlation with the mearurements with obesity in the female aged 18～47 years old. It is a more simple and practical method for measttrement of visceral fat.]]> 2018/4/27 8:23:45 WAN Li-kai, HE Bing, Tan Wei-hong,et al. 729true ［Abstract］　Objective　To explore the role of blood conservation of mild hypothermia and low flow cardiopulmonary bypass(CPB) in beating heart surgery under direct vision.Methods　Thirty patients who were eligible for beating heart mitral valve replacement surgery, according to different CPB temperature and flow, were randomly divided into two groups: experimental group: mild hypothermia (nasal temperature 30～32 ℃), reducing the flow rate 2.0～2.2 L/(min·m²); control group:the traditional beating CPB(nasal temperature 33～35 ℃),flow rate 2.6～3.2 L/（min·m²）. Jugular venous blood was taken to check the plasma free hemoglobin content before CPB and at 20, 40, 60 min after CPB.Results　At 20 min, 40 min, 60 min after CPB, the plasma free hemoglobin values(mg/L) in the experimental group was (243.73±104.52), (342.33±115.78), (386.13±122.79) while (513.60±213.47), (607.53±291.93), (742.80±282.74) in control group. The plasma free hemoglobin values between two groups at each time point were compared, and the difference was statistically significant.Conclusion　Mild hypothermia and low flow CPB in the beating heart surgery under direct vision has the role of blood conservation.]]> 2018/4/27 8:23:45 HUANG Ai-lan, LIN Hui, WEN Hong, et al. 728true ［Abstract］　Objective　To study the value of magnetic resonance imaging (MRI) and color Doppler ultrasonography ( CDU) in the diagnosis of occult residual or relapse cervical lymph node metastasis after radiotherapy and/or chemotherapy for nasopharyngeal carcinoma.Methods　Fifty-six patients (60 sides) were studied double-blindedly by MRI,CDU and physical palpation prior to cervical lymphadenctomy, and the results were contrasted with histopathologic findings.Results　According to histologic examination of the dissected specimens, in 43 of 60 sides there were occult residual or relapse cervical lymph node metastasis, 30 of 43 sides with occult residual or relapse cervical lymph node metastasis were diagnosed by manual palpation (sensitivity 69.77 %; specificity 64.70 %; accuracy 68.33% ). However, residual or relapse cervical lymph node metastasis in 40 sides were revealed by MRI(sensitivity 93.02%; specificity 88.89%; accuracy 91.66%) and MRI discovered 76.9%(10/13) of occult residual or relapse cervical lymph node metastasis which were not found in physical palpation. Residual or relapse cervical lymph node metastasis were diagnosed accurately in 39 sides by CDU ( sensitivity 92.85%; specificity 76.47%; accuracy 86.67%), and 69.23%(9/13) of the occult residual or relapse cervical lymph node metastasis which were not found in physical palpation were found by CDU.Conclusion　MRI,CDU examinations are superior to physical palpation in ascertaining occult residual or relapse cervical lymph node metastasis after radiotherapy and/or chemotherapy for nasopharyngeal carcinoma.]]> 2018/4/27 8:23:45 HE Cheng-cheng,SI Yong-feng,MA Long-bai,et al. 727true ［Abstract］　Objective　To explore clinical value of laparoscopy combined with choledochoscopy in preservation of cholecyst andremoval of calculus.Methods　Sixty patients with gallstone were divided into observation group and the control group，the observation group(n=30) received laparoscopy combined with choledochoscopy for preservation of cholecyst and removal of calculus,and the control group(n=30) received traditional laparoscoic cystectomy.The intraoperative bleeding volume, operative time, postoperative time, duration of hospitalization and complications were observed.Results　All patients were successfully completed surgery. The intraoperative bleeding volume, operative time, postoperative time, duration of hospitalization in the observation group were less than those in the control group,the difference between the groups was significant(all P<0.01), No complications was found in the observation group; In the control group，biliary fistuta was found in 2 cases(6.67%), there was no postoperative pulmonary infection and incision infection was found.Conclusion　The laparoscopy combined with choledochoscopy in preservation of cholecyst and removal of calculus is a safe and effective surgery.]]> 2018/4/27 8:23:45 LI Yong-cheng, PAN Jia-zhong, HUANG Gui, et al. 726true ［Abstract］　Objective　To compare the changes of pulmonary function indicators in the patients before and at one week after simultaneous massive whole lung lavage, to observed the impact of whole lung lavage on pulmonary function in pneumoconiosis patients in short time.Methods　The self-controled methord were used in 50 patiens who received massive whole lung lavage and were divided into normal group(n=27) and abnormal group(n=23) accoding to pulmonary function criteria to do the comparison of preoperative and postoperative indicators of one week respectively.Results　VC, TLC, FVC at one week after lavage in normal group of pulmonary function were reduced compared to those before lavage（P<0.05）；There was no statistically significant difference in other indicators before and after lavage(P> 0.05);There was no statistically significant difference before and after lavage in the abmormal group of pulmonary function（P>0.05）；The mean value of reduced indicators after the lavage were in the normal range.Conclusion　Although pulmonary function indictors were reduced after lavage but the mean value was in the normal range.There′re no adverse effects of whole lung lavage on pulmonary function in the patients with pneumoconiosis.]]> 2018/1/29 9:17:01 GUO Xian-yong,WANG Yue-gang,LI Guo-feng,et al. 725true ［Abstract］　Objective　To study the therapeutic effectiveness and associated complications of allogeneic peripheral blood stem cells transplantation(Allo-PBSCT)in treatment of acute monocytic leukemia（M₅）.Methods　Sixteen patients with acute monocytic leukemia received allo-PBSCT,11 patients received PBSCT from human leucocyte antigen（HLA）-matched sibling donors and 5 from HLA-matched unrelated donors. Pre-Processing regimen: 9 patients were subjected to modified BU/CY2 regimen which was myeloablative， another 7 patients were subjected to FBC regimen which was non-myeloablative. The graft-versus-host disease(GVHD) was prevented by cyclosporin A（CsA），short-term methotrexate（MTX）in 11 patients who received PBSCT from HLA-matched sibling donors, and another 5 patients additionally received antithymocyte globulin(ATG) and mycophenolate mofetil(MMF).A median of 6.58×10⁸/kg nucleated cells and 4.46×10⁶/kg CD34⁺ cells were transfused.Results　Except for 1 patient died early after transplantation because of hepatic veno-occlusie disease(HVOD).Other 15 patients achieved engraftment confirmed by blood type, chromosome test and DNA polymorphism，with median times of neutrophil >0.5×10⁹/L and platelet >20×10⁹/L being 13（9～17）d and 16（8～26）d respectively.Six patients developed acute GVHD(4 developed gradeⅠand 2 developed gradeⅡ).Seven patients experienced local chronic GVHD.Ten patients were found to have disease-free survival(DFS), median of 45 （3～78）months.Conclusion　Allo-PBSCT was an effective therapy for acute monocytic leukemia，it was safe and feasible，and long term survival was hoped for patients of this type.]]> 2018/1/29 9:17:02 LI Yu-miao，MAO Ping，WANG Shun-qing, et al. 724true ［Abstract］　Objective　To observe the differences of computed tomography angiography(CTA) and digital subtraction cerebral angiography(DSA) in the evaluation of cerebrovascular situation of the patients with transient ischemic attack(TIA).Methods　Seventy-four patients with TIA were examined by CTA and DSA, then the differences of results were observed.Results　In the internal carotid artery system TIA, there was no statistically significant difference in the sensitivity and specificity between CTA and DSA(P>0.05); And in the vertebral- basilar artery system TIA, the sensitivity and specificity of DSA was higher than that of CTA,the difference was statistically significant(P<0.05); But on the aspect of the degree of vascular stenosis,DSA was superior to CTA, the difference was statistically significant(P<0.05). Compared with DSA,the consuming time and incidence of complications were lower in CTA(P<0.01 and P<0.05,respectively), and the inspecting cost was lower obviously.Conclusion　CTA is a screening method for assessment of cerebral vascular status in the internal carotid artery system TIA, but the DSA is still the best choice in assessment of cerebral vascular the status in the vertebral-basilar artery system TIA.]]> 2018/1/29 9:17:02 ZHONG Liang,ZHONG You-an,CHEN Hong-mei,et al. 723true ［Abstract］　Objective　To investigate the prevalence rate of cholelithiasis and its relationship with gender and age in the elderly of health examination population in Nanning.Methods　The prevalence rate of cholelithiasis of 2 257 cases in 2011，ranging from 60 to 93 years old，were investigated. According to the age, the cases were divided to 3 groups: group A：the age from 60 to 69 years，group B：the age from 70 to 79 years，group C：the age ≥80 years，The data was analysed by SPSS17.0.Results　The total prevalence rate of cholelithiasis was 12.5%, and all patients suffered from gallbladder stones except 2 cases with bile duct stone. In both of the total and each group, female prevalence rate of cholelithiasis was significantly higher than male′s(P<0.01).Female prevalence rate of cholelithiasis was increased with age(P<0.05),and the 80～93 age group was the highest. Male prevalence rate of cholelithiasis was not related with age(P>0.05).Conclusion　Most of the cholelithiasis in the elderly of health examination population in Nanning are gallbladder stones, and the proportion of bile duct stones is very low. Female prevalence rate of cholelithiasis was significantly higher than male′s. Female prevalence rate of cholelithiasis was increased with age.]]> 2018/1/29 9:17:02 LI Kun, HUANG Ding-gui, LIU Tian-qi 722true ［Abstract］　Objective　To observe the efficacy of eye point massage in Chinese drug iontherapy for children amblyopia.Methods　One hundred and twenty amblyopia patients(224 eyes) were randomly divided into control group with 60 patients(110 eyes) and observation group with 60 patients(114 eyes).Both of two groups were got their eyeglasses by mydriatic refractometry after 1% atropine, and treated with occlusion and penalization. At the same time,control group were iontophoresed Chinese drugs each time 15 min, observation group were massaged the eye point each massage time 10 min after iontophoresing Chinese drugs, observing for 2 courses and 3 months made up of a course. The eyesight, onset time and duration were compared between the two groups.Results　The effective rate for observation group was 95.6%,the control group 91.8%,showing that was no significant difference (P>0.05) between the two groups.But the onset time and duration of observation group were better than that of control group.Conclusion　Eye point massage has similar action with Chinese drug inotherapy on the basis of comprehensive conventional therapy in the treatment of children amblyopia，and the combination of the two therapies has better effect，indicating eye point massage can speed up recovery of visual function of kids with amblyopia.]]> 2018/1/29 9:17:02 WEI Li-jiao, HE Bi-hua,XIE Xiang-yong 721true ［Abstract］　Objective　To discuss the clinical value of early tracheotomy combined with hyperventilation in severe traumatic brain injury(sTBI).Methods　Eighty patients with sTBI(GCS 3～8) were randomly divided into the observation group and the control group. Within 24 h after admission the observation group′s patients received percutaneous tracheostomy,and in a short time maintained the light to moderate hyperventilation with ventilator.Monitoring the end-tidal carbon dioxide partial pressure(PetCO₂),the PetCO₂ was maintained at 27～32 mmHg,each time for 30 min,4 times a day,for 7 days.Continuous moderate oxygen supply via face mask was used in control group′s patients after admission until respiratory failure comes out,then the percutaneous tracheostomy and mechanical ventilation(not hyperventilation was performed). All of the 80 patients received the treatment of preventing infections, reducing the intracranial pressure, cerebral protection, nutritional supporting and other symptomatic and supportive therapy. Within 7 days the changes of intracranial pressure(ICP) in the patients were continuously observed and 6 months after injury, the efficacy was evaluated according to GOS valuation method including 5 grades: good, moderate disability, severe disability, vegetative state, and death.Results　ICP decreased more rapidly in the observation group than the control group, and the difference was statistically significant(P<0.01). After 6 months there were significant differences between the two groups in good, moderate disability, severe disability, vegetative state(P<0.05),without statistically significant difference in mortality rate(P>0.05).Conclusion　The early tracheotomy combined with mild to moderate hyperventilation may be beneficial to decreasing ICP, and improving the survival quality for patients within 6 months after sTBI.]]> 2018/1/29 9:17:02 HUANG Peng, HUANG Zhai, QIN Wen-bo,et al. 720true ［Abstract］　Objective　To investigate the feasibility of immediate implant fellowing extraction of anterior tooth with using membrane guided bone regeneration technique.Methods　Thirty-two anterior teeth were pulled out from 25 patients in the surgery, and the immediate implants bone defect area was filled with artificial bone material. And then the barrier membrane was used to close it. After 4 to 6 months crown restoration were performed.The effects were evaluated through clinical observation and radiological examination.Results　Among 32 implants there was only one tooth failed due to infection, the other function was well after implant surgery. After the crown restoration it was carried one year without any loose.Conclusion　By the membrane guided bone regeneration technique the immediate implant of anterior teeth can be conducted which resolves the bone defect of lip side after the anterior teeth is pulled out. The result of immediately implant placement can reduce the treatment period so as to achieve satisfying effect.]]> 2018/1/29 9:17:02 TANG Zeng-bin,WEI Hui-ping,SHEN Ning 719true ［Abstract］　Objective　To explore the early effect of super early enteral nutrition support in patients with severe craniocerebral injury.Methods　According to the hospitalized single or double number,60 patients were divided into treatment group and control group, 30 patients in each group. the control group with was treated conventional therapy. The treatment group received supwer early enteral nutrition support, the efficacy of blood biochemical indicators, complications and prognosis were observed and compared.Results　At third week after treatment,the dicreased values in body weight(BW), triceps skinfold(TSF), arm muscle circumference(AMC), NB(g/d) in the treatment group were less than those control group(P<0.05). The levels of albumin, hemoglobin serum protein and the GOS scores in the treatment group were higher than those in the control group(P<0.05), the incidence in pulmonary infection, abdominal distension, diarrhea, gastrointestinal bleeding and the mortality rates in the treatment group were lower than those in the control group(P<0.01 or P<0.05).Conclusion　For patients with severe craniocerebral injury, super early enteral nutrition support can promote recovery, improve nitrogen balance and the nutritional status of the patients, help neural function defect rehabilitation and synthesis of skeletal muscle, improve immunity, thereby reducing the pulmonary infection and other complications, reducing the mortality rate.]]> 2018/1/29 9:17:02 YAO Zhen-ying 718true ［Abstract］　Objective　To evaluate the effects of simvastatin on cell proliferation and immune escape of human non-small cell lung cancer(NSCLC) as well as the underlying mechanisms.Methods　Cell proliferation was determined using a modified MTT assay after the treatment with different concentrations of simvastatin, or RhoA siRNA in A549 cells. The gene expression of anti-immune escape factor, major histocompatibility complex class Ⅰ(MHCⅠ, also known as HLA-A) and peptide transporter protein(TAP1) were detected by real-time RT-PCR. The change of RhoA activity was measured by a microplate reader in A549 cells treated with simvastatin, or RhoA siRNA. SiRNA transfection was done using lipofectamine 2000.Results　Simvastatin(10～30 μmol/L) significantly reduced the proliferation, enhanced gene expression of HLA-A and TAP1 and inhibited RhoA activity of A549 cells in concentration-dependent manner(P<0.05 or P<0.01). Moreover, inhibition of RhoA activity with 0.1 μmol/L RhoA siRNA also resulted in a substantial inhibition of proliferation and increased gene expression of HLA-A and TAP1 of 549 cells(P<0.01). However, combination treatment of RhoA siRNA and simvastatin exerted no synergistic effects in 549 cells compared with the specific siRNA treatment alone(P>0.05).Conclusion　Simvastatin can suppress A549 cells proliferation and immune escape primarily through inhibition of RhoA activity and thereby increasing the gene expression of HLA-A and TAP1.]]> 2018/2/5 8:39:47 LIU Bing，YANG Jie 717true ［Abstract］　Objective　To explore the value of the combined detection of Toll-like receptor-2(TLR2) and interferon-γ(IFN-γ) in pleural effusion in the diagnosis of tuberculous pleural effusion.Methods　The concentrations of soluble TLR2 and IFN-γ in pleural effusion and serum were determined by enzyme linked immunosorbent assay(ELISA) in patients with tuberculous, malignant and bacterial pleural effusion.Results　（1）The concentrations of sTLR2［22.19±2.12）ng/L］ and IFN-γ［(2 287.20±408.34)ng/L］ in pleural effusions were significantly higher than those in serum of the same patients. The levels of sTLR2 and IFN-γ in tuberculous pleural effusion was significantly higher than those in malignant and in bacterial pleural effusion.（2）The ROC analysis for TLR2 and IFN-γ were done. The levels of TLR2 generated an area under the receive operating characteristic(ROC) curve of 0.696［95% confidence interval (CI):0.501～0.849］, at a sensitivity of 66.67%, a specificity of 80%. As well as IFN-γ generated an area under the ROC curve of 0.945(95%CI:0.875～1.014), at a sensitivity of 87.5%, a specificity of 97.9 %.Conclusion　The combined decection of TLR2 and IFN-γ in pleural effusion may be helpful in the differential diagnosis of tuberculous pleural effusion and the other pleural effusion, which provides an important information for clinical diagnosis and treatment.]]> 2018/2/5 8:39:47 HUANG Lu-ying, WU Yan-bin, FENG Ting-mei, et al. 716true ［Abstract］　Objective　To explore the effects of the change of Glucagon-like peptide-1 level on glucagon and early phase insulin secretion in the patients with the newly diagnosed type 2 diabetes.Methods　Patients with the newly diagnosed type 2 diabetes(T2DM group) and healthy volunteers(control group) were used as the research object, and the standardized bread meal test was used to observe effects of the dynamic changes of GLP-1 level at 0 min, 30 min and 120 min on plasma glucose, glucagon and insulin secretion.Results　The levels of GLP-1 at each time point in newly diagnosed T2DM group were significantly reduced compared with those of the control group respectively(P<0.05); The levels of insulin at 30 min and 120 min were both significantly reduced compared with those of the control group respectively (P<0.05),but the difference had no statistical significance at 0 min between two groups(P>0.05);The levels of glucagon at each time point in newly diagnosed T2DM group were significantly higher than that of the control group(P<0.05). The first-phase insulin secretion index of newly diagnosed T2DM group was significantly reduced compared with those of the control group(P<0.05).Conclusion　The levels of GLP-1 at each time point in newly diagnosed patients with type 2 diabetes mellitus were significantly reduced;GLP-1 dificiency might be the important factors of lack of islet beta cell secretion and insrease of glucagon secretion.]]> 2018/2/5 8:39:47 WU Mei-fen, WU Ge, Cai Xiao-ling,et al. 715true ［Abstract］　Objective　To investigate the effect of metformin on proliferation, cell cycle distribution and apoptosis of human endometrial carcinoma cell lines in vitro.Methods　The effects of metformin on proliferation, cell cycle distribution and apoptosis of Ishikawa cell lines were measured by MTT assay and fluorescence-activated cell sorting technique.Results　Metformin potently inhibited growth in a dose-dependent manner in Ishikawa cell lines in a dose- and time- dependent fashion. Flow cytometric analysis showed that metformin increased the number of cells in G₀/G₁ and reduced the percentage of cells in S phase.Conclusion　Metformin is a potent inhibitor of cell proliferation in endometrial cancer cell lines.]]> 2018/2/5 8:39:47 SONG Hong-lin, LIANG Shao-feng 714true ［Abstract］　Objective　To investigate the effect of GnRH antagonist in IVF-ET on IL-1β in follicular fluid and the relationship between GnRH antagonist and IVF-ET outcome in polycystic ovarian syndrome(PCOS) patients.Methods　Seventy-six cases of PCOS patients undergoing IVF-ET between August 2011 and September 2012 were divided randomly into two groups: Group A(study group): 28 patients with PCOS were subjected to a protocol of gonadotropin-releasing hormone antagonist. Group B(control group): 48 patients with PCOS were subjected to a long protocol of gonadotropin-releasing hormone agonist. The level of IL-β of the two groups and the relationships between GnRH antagonist and IVF-ET outcome were analyzed.Results　The two groups were similar with respect to IL-1β in follicular fluid(P>0.05); the two groups were similar with respect to endometrium thickness, the number of oocyte, the mature rate of the oocytes, fertilization rates, cleavage rates, the rate of the high-quality embryos, implantation rate, pregnancy rate(P>0.05). The doses of gonadotropin and the days of administration in Group A was less than those in Group B(P<0.01). The levels of serum E2 on day of HCG administration, and the cycle cancellation rate in Group A was lower than that in Group B(P<0.05 or 0.01). The difference of the incidence rate of OHSS was not significant(P>0.05) between the two groups.Conclusion　It is demonstrated that GnRH antagonist in PCOS patients undergoing IVF-ET did not alter levels of IL-1β in follicular fluid, the development of follicle and the oocyte competence. These results demonstrated the high-performance and safety of this drug on controlled ovarian stimulation protocol.]]> 2018/2/5 8:39:47 HUANG Ying, QIU Ying, TAN Xiu-qun 713true ［Abstract］　Objective　To analyze the clinical characteristics, treatment and prognosis of human immunodeficiency virus antibody positive versus negative patients with cryptococcal meningitis.Methods　The clinical data of 64 patients with cryptococcal meningitis who were treated in our hospital from June 2006 to June 2012 was collected. These patients were divided into 2 groups according to positive and negative for comparing and analyzing their characteristics.Results　Both groups had the signs of fever, headache and emesis, while companying meningeal irritation and increased intracranial pressure. Compared with HIV negative group, in HIV positive group, onset was faster, fever was more obvious, the time from onset to confirmation was shorter, and with more merging opportunistic infections. In both groups,cerebrospinal fluid examinations showed cell population and protein content increased, glucose and chloride content decreased. Cerebrospinal fluid cell population of HIV positive group was less than that of HIV negative group. In both groups head imagings showed meningeal change and hydrocephalus, while damaging brain parenchyma was found more in HIV positive group. Both groups were treated by single amphotericin B or combination of amphotericin B and other antibiotic therapy. The mortality was higher in patients who was treated without antibiotic therapy and whose intracranial pressure was higher. There were 17 patients died in HIV positive group, 2 patients died in HIV negative group. There was no statisically significant difference in mortality between two groups（P>0.05）.Conclusion　The clinical characteristics and laboratory test results of both groups are similar. Amphotericin B combined with other antibiotic therapy has a better therapeutic effect, early diagnosis and actively controlling intracranial pressure contribute to improving prognosis.]]> 2018/2/5 8:39:47 LI Wu-ming,HU Shui-xiu,LI Hong-ru 712true ［Abstract］　Objective　To investigate the changes of electrocardiographic P wave in patients with paroxysmal atrial fibrillation.Methods　Two hundred patients were divided into 2 groups:paroxysmal atrial fibrillation group(n=100) as group PAF: patients with paroxysmal atrial fibrillation; control group (n=100) as group no-PAF: patients with no atrial fibrillation. P wave duration, P terminal force in lead V1 (Ptfv1), left atrial diameter(LAD), left ventricular diameter(LVD) and left ventricular ejection fraction(LVEF) were detected by electrocardiography and echocardiography.Results　P wave duration was significantly longer in group PAF ［(120±10)ms, P<0.01］ than in group no-PAF (99±11)ms. Ptfv1 was greater in group PAF (0.051±0.023)mm·s than in group no-PAF ［(0.026±0.010）mm·s, P<0.01］. There were no diffenrences in the size of left atrium and ventricular between two groups（P>0.05）, the LVEF was also in the same level between two groups（P>0.05）.Conclusion　Paroxysmal atrial fibrillation may induce the increased P wave duration and increase Ptfv1, which is independent of the size of left atrium.]]> 2018/2/5 8:39:47 WEI Kai-fu, QIN Shao-ming, LIN Ying-zhong 711true ［Abstract］　Objective　To study the role of dietary intervention in the treatment of chronic pharyngitis.Methods　One hundred patients with typical chronic pharyngitis were randomly divided into intervention group and control group(50 patients for each group).The patients in control group received regular drug treatment. Besides regular drug treatment, the patients in intervention group received nutritional regulation, health education, and so on. The results of the two groups were compared.Results　In the intervention group, 41 patients were cured, marked effect was found in 5 patients, effectiveness in 4 patients; In control group, 38 patients were cured, marked effect was found in 6 patients, effectiveness in 6 patients. There was no statistically significant difference between the two groups(P>0.05). One year follow-up showed, recurrence was found in 15 patients in the intervention group, and that in 40 patients in the control group. The recurrence rate was less in intervention group than that in the control group, the difference had statistically significant between the two groups(P<0.05).Conclusion　Dietary intervention can make patients mastering right dietary methods and changing a bad dietary habits. It can effectively decrease the recurrence rate of chronic pharyngitis.]]> 2018/2/5 8:39:47 MENG Hui-ju, LIANG Yu, QIN Yan-yi, et al. 710true ［Abstract］　Objective　To study the effects of YLS polysaccharides on intracellular calcium concentration in AD cell model.Methods　PC12 cells were treated with Aβ_25-35 to establish a cell model of Alzheimer’s disease, After pretreated with Huperzine and YLSP, the concentration of intracellular calcium were examined by FCM.Results　The concentration of intracellular calcium in the model group were increased significantly compared with the control group(P<0.01), in the Huperzine and YLSP groups, concentration of intracellular calcium were observed obviously lower(P<0.01) than the model group, and the high-dose YLSP group was lower than Huperzine group(P<0.01).Conclusion　YLSP protect PC12 cells from calcium overload damage induced by Aβ_25-35.]]> 2018/2/5 8:39:26 CHEN Xiao-yu, HUANG Ren-bin 709true ［Abstract］　Objective　To explore the characteristics of Cushing′s syndrome in clinical manifestations and endocrinological inspection and imaging examination,and evaluate the efficiencies of various assays in diagnosis and differential diagnosis.Methods　The medical records of a total of 42 patients with Cushing′s syndrome were retrospectively reviewed.Results　Cushing′s disease was the most common cause of Cushing′s syndrome. About 64.3% patients had typical Cushing symptoms. The prevalence of glucose intolerance and hypertension were 61.9% and 61.9%， respectively. The sensitivity for diagnosis from high to low was lack of cortisol suppression after 1 mg overnight dexamethasone test，low-dose dexamethasone inhibitory test,increased 24 h urinary free cortisol，loss of the normal diurnal rhythm of the plasma cortisol，raised morning plasma cortiso1.In the differential diagnosis,93.3% patients with adrenal adenoma can not be suppressed by high dose dexamethasone suppression test,but 71.4% patients with Cushing′s disease can be suppressed.In the imaging examination, CT of the adrenal glands can detect 100% adrenal neoplasm.Conclusion　Cushing′s disease and adrenal adenoma are the most common causes in Cushing′s syndrome.We can concern about the tendentious clinical manifestations of Cushing′s syndrome. A combination of ACTH， 8 mg dexamethasone suppression test as well as the image assay is necessary for the differential diagnosis for Cushing′s syndrome.]]> 2018/2/5 8:39:26 ZHONG Mei,YAN Xiao-dong,LU Wen-sheng,et al. 708true ［Abstract］　Objective　To understand the patient satisfaction about the tuberculosis designated hospital control mode, in order to provide the reference for improving medical services in the future.Methods　According to the unified survey plan which was drafted by the global fund tuberculosis project department, twenty patients who were registered before August 1, 2011 were selected, and the questionnaire survey was carried out in the tuberculosis project site Lingui county.Results　35% of the patients got family medicine supervision during the process of TB treatment ；more than 90% of the patient said “satisfaction” and “general” to the evaluations about the environment of outpatient, the service attitude of health care providers, the process of seeing a doctor, the interpretations of TB knowledge and so on; 100% of the patient said “trust” to the evaluation about the technology of the doctor ；50% of the patient said “yes” to the question “whether or not affected your medication adherence after what the doctor told you?”; 95% of the patient said “yes” to the question “whether or not afford all the cost of the treatment you got ?”;95% of the patient said “yes” to the question “ whether or not willing to recommend your relatives or friends to the designated hospital when they were suspected tuberculosis?”.Conclusion　The implementation of the tuberculosis designated hospital control mode was good on the whole, most of patients were satisfied on the medical process. But it is necessary to explore practical medication management mode, in order to make the patients to regularly taking medicine by DOT strategy.]]> 2018/2/5 8:39:26 WU Teng-yan, LIU Fei-ying, WEI Suo-su, et al. 707true ［Abstract］　Objective　To detect changes of T lymphocyte subsets (CD3⁺CD4⁺,CD3⁺CD8⁺,CD4/CD8) and regulatory T cells(Treg,CD4⁺CD25^+highCD127^low) in adult patients with steroid-sensitive nephrotic syndrome(SSNS),and investigate dynamic change trend and clinical significance in different stages of primary nephrotic syndrome (PNS).Methods　Thirty-five SSNS patients enrolled in SSNS group were inpatients in our department first treated with prednisone (1 mg·kg^-1·d^-1) for eight weeks, according to clinical remission of PNS. Thirty normal persons were served as healthy control group. Regular laboratory examinations were detected before treatment (W0 group), 4-week treatment(W4 group ) and 8-week treatment (W8 group), such as proteinuria, blood albumin（Alb）, serum creatinine(Scr), cholesterol(Cho) and triglycerides(TG). T lymphocyte subsets and Treg were detected by flow cytometry.Results　（1）Compared with W0 group, proteinuria, Cho, TG and Scr decreased significantly(P<0.05),while Alb increased significantly(P<0.05) in W4 group and W8 group. Alb in W8 group was higher than that of W4 group(P<0.05).There were no significant differences in above other indexes between W4 group and W8 group (P>0.05).（2）Compared with control group,CD4% and CD4/CD8 ratio increased, while Treg and Treg/CD4 ratio decreased slightly in SSNS group before treatment, but there were no significant differences(P>0.05). Compared with control group, CD4% and the ratio of CD4/CD8 increased(P<0.05), while the ratio of Treg/CD4 decreased significantly (P<0.05) in W4 group. After 8-week treatment, there were no significant differences of CD4%, CD8%, CD4/CD8 ratio, Treg, and Treg/CD4 ratio(P>0.05), compared with control group.（3）There were dynamic changes during the treatment in SSNS group. Compared with W4 group, CD4% and CD4/CD8 ratio were lower（P<0.05）, while CD8%,Treg and Treg/CD4 ratio were higher（P<0.05）in W8 group. But there were no significant differences between W8 group and control group.Conclusion　There are changes of T cell populations, T lymphocyte subsets and Treg/CD4 ratio imbalance in PNS adult patients. The disorder immune state even sustained after 4-week treatment of glucocorticoid. It ameliorated after 8-week treatment of glucocorticoid with complete remission. Regulatory T lymphocytes may play an important role in the remission of patients with SSNS.]]> 2018/2/5 8:39:26 PENG Xiao-mei,YE Kun, XIONG Li-jia, et al. 706true ［Abstract］　Objective　To test the level of serum insulin (INS), C-peptide (C-P), glucagon (GLC) and somatostatin (SS) in the type 2 diabetes mellitus(T2DM) patients and the control group in order to find out the possible mechanisms of pancreas islet function impairment in T2DM patients.Methods　Forty T2DM patients were used as T2DM group and 19 healthy subjects as control group. All subjects fasted for 10 hours, and received 75 g oral glucose tolerance test(OGTT) at 07:00 hrs. Then the level of PG, INS, C-P, GLC and SS were tested at baseline, 30 mins and 120 mins after oral glucose. The level of PG, INS, C-P, GLC and SS were compared between 2 groups statistically.Results　（1）Compared with the control group, the level of INS and C-P decreased significantly in T2DM group(P<0.05).（2）The level of GLC at different time point was higher in the T2DM group than that in the control group(P<0.01).（3）The SS level at 30 and 120 mins after glucose intake in the T2DM groups were significantly higher than that at baseline compared with the control group, the SS level was lower at 30 mins(P<0.05) and higher at 120 mins(P<0.01) in T2DM group.Conclusion　Compared with healthy people the level of the INS and C-P decreased and the level of GLC increased in the T2DM patients. The secretion of the SS in T2DM patients is disorderly.]]> 2018/2/5 8:39:26 XU Lin, XIAO Zheng-hua, CHEN Ding-yu 705true ［Abstract］　Objective　To compare the diagnostic accuracy of pleural SLPI and ADA for tuberculous pleural effusion.Methods　Pleural effusion samples were obtained from 103 patients who presented to the department of respiratory diseases(45 in tuberculous pleural effusion group，31 in malignant pleural effusion group，16 in bacterial pleural effusion group and 11 in transudative pleural effusion group). The levels of SLPI in pleural effusion and serum were determined by sandwich enzyme- linked immunosorbent assays(ELISA). ADA activity was determined using the colorimetric method kit.Results　(1)The concentrations of SLPI in tuberculous pleural effusion(193 790±15 476)pg/ml was higher than that in malignant group(121 700±13 101)pg/ml, in bacterial group(92 885±26 962)pg/ml and in transudative group(109 360±21 619)pg/ml(P<0.05). But there were no statistical significance among the malignant group. bacterial group and transudative group(P>0.05). The concentration of pleural ADA in tuberculous pleural effusion group and bacterial group was higher than that in malignant group and transudative group(P<0.05).(2)The cut-off value of pleural SLPI for the diagnosis of tuberculous PE was 236 071 pg/ml with the corresponding sensitivity and specificity 43.2% and 91.4%，respectively. The cut-off value of pleural ADA for the diagnosis of tuberculous PE was 29.5 U/L with the corresponding sensitivity and specificity 75.0% and 64.2%，respectively. The combinations of SLPI and ADA increased specificity to 95%. The combinations of SLPI or ADA increased sensitivity to 89%.Conclusion　Pleural SLPI and ADA may be helpful for the differential diagnosis of tuberculous pleural effusion and the other pleural effusion. The combinations of SLPI or/and ADA further increased diagnostic performance.]]> 2018/2/5 8:39:26 WU Yan-bin，LIANG Xiang-dong，WU Cong，et al. 704true ［Abstract］　Objective　To explore more the clinical curative effect of domperidone combined with touch in the treatment of premature infant with feeding intolerance.Methods　Sixty premature infants with feeding intolerance born in 28 to 37 gestational weeks were randomly divided into the treatment group(32 cases) and the control group(28 cases). Two groups were given conventional treatment and domperidone on the basis of above treatment,the treatment group received touch.The effect of the two groups after treatment were observed.Results　The total effective rate(90.6%) of the treatment group was better than that(67.9%) of control group(P<0.05), After treatment, the treatment group was superior to the control group in relief of symptoms and weight recovery(P<0.01).Conclusion　Domperidone combined with touch intervention in the treatment of premature infant with feeding intolerance can improve the curative effect,and the survival success rate of premature infants.]]> 2018/2/5 8:39:26 YE Li-hua, TANG Hai-yan, PANG Xiao-jun,et al. 703true ［Abstract］　Objective　To explore the clinical curative effect of wound care membranes in the treatment of ulcerative bedsore.Methods　One hundred and twenty-six patients with ulcerative bedsore treated in orthopaedics department in our hospital from February 2007 to June 2012 were randomly divided into two groups, the research group(68 cases) was treated by wound care membrane and the control group(58 cases) were treated by moist exposed burn ointment. The clinical curative effect, wound healing time, length of stay in hospital and the charge were compared between the two groups.Results　The wound healing time, the length of stay of the research group are significantly shorter than those of the control group（P<0.01）; hospital cost of the research group was lower than that of the control group（P<0.01）; clinical curative effect of the research group was obviously higher than that of the control group（P<0.05）.Conclusion　Treatment of ulcerative bedsore by wound care membrane can accelerate wound healing of bedsore, significantly shorten the period of treatment and improve the cure rate, and the method is simple, which is worth clinical promotion.]]> 2018/2/5 8:39:26 LAI Ming-chun, ZHANG Qin-cai, WU Yun-qi 702true ［Abstract］　Objective　To investigate the composition of renal complex calculi in Nanning area in order to provide a basis for its treatment and prevention.Methods　Infrared spectrum analysis of the stones from 98 patients with renal complex calculi in our hospital was performed and the related clinical data about the patients were collected.Results　Most(93, 94.9%) of the renal complex calculi in the 98 patients were mixed calculi or infection stones, including calcium oxalate monohydrate(2,2.0%), calcium oxalate monohydrate plus carbonate apatite(20,20.4%), calcium oxalate monohydrate and dehydrate plus carbonate apatite(16,16.3%),calcium oxalate monohydrate plus anhydrous uric acid(12,12.2%), calcium oxalate monohydrate plus carbonate apatite plus anhydrous uric acid(13, 13.3%),calcium calculi plus infection stones(17, 17.3%), infection stones(13, 13.3%).Conclusion　Most of the large renal complex calculi are mixed calculi oxalate or infection stones, and carbonate apatite, uric acid and magnesium ammonium phosphate are their compositions. during its growth, many facts are involved. It should be necessary to perform an active preventive treatment aimed at different pathological courses.]]> 2018/2/5 8:39:26 HUANG Heng-qian, FU Jie-xin, XIE Guang-yu, et al. 701true ［Abstract］　Objective　To explore the mechanism of dental and craniofacial changes in Class Ⅱ Jaw′s malocclusion being treated nonextractedly in straight wire arch technique with multiloop edgewise arch wire and microimplant anchorage.Methods　Experimental group included 13 cases being treated nonextractedly in straight wire arch technique,using multi-loop edgewise arch wire and microimplant anchorage. Control group included 15 cases being treated extractedly in straight wire arch technique, using elastic pull of Class Ⅱ.All Cases were skeletal facial type of Class Ⅱ and mandibular average angle,light or middle dentition crowding,15 to 32 years old(average 23.6 years old). Cepholometric values before and after treatment in two groups had been compared statisticlly in SPSS17.0.Results　Angles SNA,ANB were smaller and SNB was bigger in experimental group（P<0.01）, distances U1-NA、L1-NB were shorter and angles U1-L1,FMA were bigger in control group after treatment（P<0.05）.Conclusion　Mechanism for treatment effect in Class Ⅱ Jaw′s malocclusion being treated nonextractedly in straight wire arch technique,using multi-loop edgewise arch wire and microimplant anchorage were as follow:（1）Jaw’discrepancy in sagittal plane had been improved because point A and upper anterior dentition was moved posteriorly and adequate space could be obtained.（2）Controlling the mandibular plane angle benefited mandibular rotation in counter clockwise and point B being moved forward.]]> 2018/2/23 15:16:37 ZHOU Yan，HUANG Min-fang, FANG Zhi-xin,et al. 700true ［Abstract］　Objective　To study the correlations between the retinal binding protein to creatinine ratio in random urine, morning voiding urine and 24 h urine and the 24 h protein.Methods　The random urine,morning voiding urine and 24 h urine of patients were collected in the same day, and retinal binding protein, microalbumin, and creatinine were detected respectively.The results of random urine and morning voiding urine should be expressed in ratios with creatinine.Results　There were good correlations of 24 h urinary retinal binding protein and 24 h urinary protein. The correlation coefficient was 0.955(P=0.000). The correlation coefficients of retinal binding protein in random urine and morning voiding urine with 24 h retinal binding protein were 0.663,0.707(P=0.000),and with 24 h urinary protein were 0.673,0.738(P=0.000). The correlation coefficients of retinal binding protein/creatinine in random urine and morning voiding urine with 24 h urine protein were 0.792,0.880(P=0.000).Conclusion　It is suggested that there is a significant correlation between the ratio of retinal binding protein/creatinine in random urine or morning voiding urine and 24 hour urinary quantitative protein,and both of the sample can be obtained easily.]]> 2018/2/23 15:16:37 YUAN Yu-lin, QIN Gui-fang, DU Wu-jie, et al. 699true ［Abstract］　Objective　To explore the perspective and practicability of the biomechanical and clinical research of lateral lumbar interbody fusion with single cage for the 4th and 5th lumbar spine.Methods　（1）The principle of units classification was used to analyze the three-dimensional finife element of lumbar spine established by a health adult man’s CT.（2）Samples of L4,5 exercise fragment of a fresh calf were taken, randomly divided into two groups and simulated two kinds of surgical methods. In the first group, right vertebra lamina was windowed and placed a cage by the lateral side of the lumbar spine. In the second group, semi-lamina of vertebra and upper and lower small joints were resected for decompression and slantly placed one cage by the proterior side and the relative displacement of the related points was measured. When the samples were exerted working load,data was recorded and statistical analysis was performed.（3）Thrity patients were collected to receive L4,5 lateral lumbar interbody fusion with cage and the operative efficacy was evaluted with JOA evaluation standard.Results　In three imensional finite element model, in the stiffness of buckling, extension, right side-bend, rotation, the lateral approach was superior to the posterior. There was no statistically significant difference in the regulation of longitudinal compress and left side-bend between two groups. In the lumbar vertebra model of the calf,the result was also that the lateral approach was superior to the posterior approach in the exercise of buckling, extension, right or left side-bend and rotation, there was dramatically statistical significance. In results of clinical research, the effective rate of operation was 100%, improvement rate 90%,none of them had a serious complication.Conclusion　From the perspective of biomechanical and clinical research the effect of implanting lumbar interbody fusion cage from lateral approach is better than from posterior. Surgery is safe and effective, which is worthy of being popularized.]]> 2018/2/23 15:16:37 YAN Dong-xue, HUANG Yong-ji, LUO Jun, et al. 698true ［Abstract］　Objective　To explore the relationship of the expression of DNA-PKcs and ERCC1 in primary epithelial ovarian cancer with the prognosis ovarian cancer.Methods　Expressions of DNA-PKcs and ERCC1 were detected in 99 samples of epithelial ovarian cancer tissues, 16 samples of normal ovary tissues by immunohistochemical SP method.Results　The positive rates of DNA-Pkcs and ERCC1 expression in epithelial ovarian cancer patients were 60.61%(60/99) and 53.54%(53/99) respectively, the positive rates of DNA-PKcs and ERCC1 in epithelial ovarian cancer tissues were apparently highter than normal ovary tissues(P<0.01); The expressions between DNA-PKcs and ERCC1 showed positive correlation(r=0.408, P=0.000); Single factor Kaplan-Meier analysis showed that the expressions of DNA-PKcs and ERCC1, the co-expression of DNA-Pkcs and ERCC1, the FIGO stage, lymph node metastasis, the half-life of serum CA125 and the diameter of residual tumor were correlated with the average survival time in ovarian cancer patients(P<0.05). The expression of ERCC1, FIGO stage, lymph node metastasis and the diameter of residual tumor were related to the independent risk factors of ovarian cancer prognosis revealed by Cox multiple factors survival analysis.Conclusion　The positive expression rates of DNA-Pkcs and ERCC1 in epithelial ovarian cancer patients were significantly higher than that in normal ovarian tissues; The combined detection of DNA-PKcs and ERCC1 in epithelial ovarian cancer patients is maybe a new target for early diagnosis and prediction of outcome for this disease.]]> 2018/2/23 15:16:37 YU Yan-li, XIONG Yan-jie, TIAN Zhi-hua, et al. 697true ［Abstract］　Objective　To investigate the sleep snoring children in Nanning and identify the causal factors.Methods　During 2007～2010 year a population-based cross-sectional study of 6 999 children, aged from 3 to 14 years was performed and analysis of 2 292 questionnaires for snoring children was performed.Results　The prevalence of sleep snoring in boy children was higher than that of girl. Snoring children with tonsillar hypertrophy (recurrent tonsillitis) was accounted for 31.02%, nasal disease 9.82%, allergic rhinitis 16.19%, repeat respiratory infection 29.89%, bronchial asthma 7.98% and craniofacial malformations (malocclusion) 3.80%. There was family history in 26.79% of children with sleep snoring. Family of 47.77% of the children with sleep snoring had a history of smoking in their father or mother. Children with sleep snoring who like the plush toys were 32.02%, children who enjoy the sleep pillow 23.56%. There were only 0.19% parents who think that sleep snoring in children may be a kind of disease.Conclusion　We concluded that sleep snoring is children common disease. It is probably useful to improve the recognition of sleep-related breathing disorder in children early based on paying attention to sleep snoring children and family genetic and environmental factors.]]> 2018/2/23 15:16:37 TAN Ying, XIE Qing-ling, TANG Xiao-yan, et al. 696true ［Abstract］　Objective　To study the effect of continuous vacuum sealing drainage (VSD) technique in the prevention of inflammatory reaction in acute soft-tissue trauma.Methods　Forty cases of acute traumatic wounds caused by various reasons were randomly divided into two groups.Twenty cases were treated by covering wounds with continuous VSD after general wound debridement (treatment group, group A) and the other 20 cases were treated by regular debridement of the wound followed by covering biological dressing A(control group, group B).The blood serum was taken by phlebotomizing to detect the content of TNF-α,IL-6 and IL-8 on preoperative and postoperative day 1,3,5.The swelling degree of postoperative wound was observed. According to the condition of wounds patients in tow groups were chosen different surgical methods for wound repair 7 days later.Results　There was no significant difference about the content of TNF-α,IL-6 and IL-8 between the two group on preoperative day（P>0.05）.On postoperative day 1,3,5, the content of TNF-α,IL-6, and IL-8 in group A was significantly lower than that in group B（P<0.05）.The average time of wound healing in group A was significantly shorter than that in group B （P<0.05）.Conclusion　Using continuous VSD technique in acute soft tissue trauma can reduce the inflammation and facilitate wound healing.]]> 2018/2/23 15:16:37 MAO Qing-long, CHEN Bin, SHOU Jia-bao, et al. 695true ［Abstract］　Objective　To discuss the clinical improvement of three-level electroencephalography monitor and microsurgery for intractable epilepsy.Methods　Thirty intractable epilepsy patients were comprehensively evaluated by combining electroencephalography(EEG)/videoelectroencephalography(VEEG) with imaging finding of computer tomography(CT)/magnetic resonance imaging(MRI)/emission computed tomography(ECT), then, the epileptic focus were located, finally, epileptic discharge area were checked by electrocorticalgram(ECoG)/depth electroencephalography(DEEG) and conformed ECoG and DEEG during wake up test. Microsurgical resection were performed for isolated epileptic focus, corpus callosotomy were performed for multifocal or bilateral independent epileptic foci, multiple subpial transectio(MST) were performed for epileptic foci included cortical functional areas.Results　Postoperative Engel grade:Ⅰ 19 cases, Ⅱ 10 cases, Ⅲ 1 case, Ⅳ none. There is on permanent nerve function loss reduction in those cases.Conclusion　Combining VEEG/MRI/ECT and ECoG(or DEEG), the epileptic focus of intractable epilepsy were accurately located, and satisfactory post-operation outcomes were achieved.]]> 2018/2/23 15:16:37 LAN Sheng-yong, XIAO Qian, XU Peng，et al. 694true ［Abstract］　Objective　To investigate the effect of using indocyanine green (ICG) angiography in anterior communicating aneurysm clipping.Methods　Surgical experience of 19 patients with anterior communicating aneurysms was analyzed retrospectively. All patients underwent ICG angiography to evaluate the aneurysm clipping and the parent arteries. Post-operative 3D-CTA was performed to conform the complete aneurysm obliteration.Results　During the microsurgical procedure, aneurysm neck remnant was found in 1 patient and perforating artery clipping in 1 patient. The ICG angiography performed after clip replacement showed that the aneurysm was completely obliterated and the parent artery and perforating artery were patent. The intraoperative angiography findings completely corresponded to the postoperative 3D-CTA.Conclusion　The high spatial and temporal resolution of the images, the simplicity and repeatability of the ICG angiography could be used to judge whether the aneurysm is completely obliterated, the parent and distal artery is stenosis, perforating arteries occlusion，and can improve the safety of the operation.]]> 2018/2/23 15:16:37 WU Song, XIAO Shao-wen, LU Gui-hua, et al. 693true ［Abstract］　Objective　To investigate the expression of SYK in NK/T cell lymphomas and its significance.Methods　The expression of SYK in NK/T cell lymphoma was examined by immunohistochemisty assay to evaluate the relation between it`s expression and clinicopathological characters such as age, sex, stage and proliferation index.Results　The percentage of NK/T-cell lymphoma with high expression of SYK was 27.27%(15/55).That with low expression of SYK was 72.73%(40/55).The expression of SYK was associated with tumour stage and cell proliferation index. NK/T lymphoma with high SYK expression had higher stage and proliferation index.Conclusion　SYK overexpression is associated with tumour stage and cell proliferation in NK/T cell lymphomas, and is a potential diagnostic and prognostic factor.]]> 2018/2/23 15:17:06 ZHANG Jie, XIAO Sheng-jun, SONG Tie-shan 692true ［Abstract］　Objective　To observe the expression and significance of Caspase-9 and Caspase-3 in CA1 area of hippocampus of vascular dementia rats.Methods　One hundred mice were randomly divided into sham operation group and vascular dementia model group. The groups were divided randomly into 1, 2, 4, 8 and 12 w groups (n=10). Vascular dementia rat models were established by blocking four vessels. Learning and memory abilities were detected by Morris water maze. Apoptotic cells were detected by TUNEL.The expression of Caspase-9 and Caspase-3 were detected by immunohistochemistry.Results　(1)Compared to sham operation group, apoptotic cells were increased in CA1 aren of hippocampus,there were statistical significance at every time point of the model group(P<0.05).(2)Compared to sham operation group,the positive expression of Caspase-9 and Caspase-3 rose at 1 w, peaked at 2 w,at 4 w began to decline, to 12 w was higher still in vascular dementia model group, there were statistical significance(P<0.05).Conclusion　Caspase-9 and Caspase-3 participated to regulate apoptosis together in hippocampal CA1 area of vascular dementia rats.]]> 2018/2/23 15:17:06 YUAN Min, LIU Bin, ZHENG Xiu- Qi 691true ［Abstract］　Objective　To analyze the effectiveness of two methods for fecal DNA extraction and detection.Methods　In 108 patients who were investigated，fecal samples were collected. The fecal DNA was extracted by QIAamp DNA Stool Mini Kit and phenol/chloroform method.The DNA quality extracted by the two methods was compared with each other.Results　The concentrations of DNA extracted by phenol/chloroform method and kit were(232.51±41.24)ng/μl and (67.35±13.17)ng/μl, respectively (P<0.01). The fecal DNA extracted by kit outweighed than phenol/ chloroform method when compared on the indexes of purity(OD260/280)，clarity of DNA solution and electrophoresis results（all P<0.01）. There were 91.67%（99/108）samples of fecal DNA extracted by kit can be amplified by PCR, while only 5%（1/20）samples of fecal DNA extracted by phenol/chloroform method can be amplified（P<0.01）. There was not enough concentration of DNA got from another 8.33%（9/108）samples of stool at the first time extracted by kit, but can achieve the requirements by improving the method at last.Conclusion　The fecal DNA may be got higher quality extracted by kit when compared with phenol/chloroform method, and can achieve the requirements of bisulfite modification and PCR.Improving the phenol/chloroform method may also get high qualitative DNA.]]> 2018/2/23 15:17:06 HE Chun-gang, Huang Qin-yuan,Chen Li-sheng, et al. 690true ［Abstract］　Objective　To investigate antibiotic resistance of methicillin-resistant Staphylococcus aureus（MRSA）from children with MRSA infection and its clinical characteristics,in order to provide information for clinic.Methods　Total of children with MRSA infection were investigated by us in the Hospital Affiliated of Guilin Medical College from March 2009 to October 2012. The strains of MRSA were identified by cefoxitin slip test, the antimicrobial sensitivity tests of MRSA to 20 kinds of drugs were tested by VITEK32 automatic microbial monitor GPS.Results　There were totally 289 strains of Staphylococcus aureus isolated from various clinical specimens obtained from 289 children, among which there were 51 strains of MRSA with the detection rate of 17.65%; The most common locus where MRSA was positive were liquor puris and secretion. The most common age with MRSA infection was 0～3 year old, which accounted 78.43%.The resistance rate to Penicillin and Oxacillin were 100%, resistance rate to Cefazolin,Cefuroxime, Cefotaxime,Ceftizoxime were higher, Clindamycin was about 53%; The sensitive rate to Sulfamethoxazole/Trimethoprim, Norfloxacin and Rifampicin were higher. No strain was found to be resistant to Vancomycin, Fusidic acid, Teicoplanin, Levofloxacin, Minocycline, Furadantin and Dalfopristin.Conclusion　The proportion of separation of samples of infants were comparatively large. The sensitive rate to Vancomycin, Fusidic acid, Teicoplanin, Dalfopristin, Sulfamethoxazole/Trimethoprim, Furadantin,Rifampicin were high in our studying,they were may be suitable for MRSA induced the infection in children in the north areas of Guilin.]]> 2018/2/23 15:17:06 WANG Qun, OU Wei-lin, GAO Yan,et al. 689true ［Abstract］　Objective　To study the value of Epstein-Barr virus DNA load detection in the outpatient with nasopharyngeal carcinoma(NPC).Methods　Sixty-one outpatients with suspected NPC were detected respectively EBV-DNA load, EBV VCA-IgA and EA-IgA, and compared with histopathological diagnosis.Results　Those cases were detected 47 cases of nasopharyngeal carcinoma through histopathology. The sensitivities of EBV-DNA load, VCA-IgA and EA-IgA were 76.6%（36/47）,55.3%（26/47）, and 45.8%（22/48） respectively; the specificities were 64.3%（9/14）,71.4%（10/14）,and 92.3%（12/13） respectively; the accuracies were 73.8%［（36+9）/61］,59.0%［（26+10）/61］, and 55.7%［（22+12）/61］respectively. Compared with histopathology, the positive rate of the three detection methods except EBV-DNA load showed no significant difference（P>0.05）, the other were significant difference（P<0.01）; The methods of EBV-DNA load, VCA-IgA and EA-IgA were performed between NPC and control groups, and among NPC clinical stages, except that the detection of EBV-DNA load was significant difference（P<0.01）, the other were no significant difference （P>0.05）.Conclusion　In three kinds of nasopharyngeal cancer screening technology, EBV-DNA load was better than EBV VCA-IgA and EA-IgA.]]> 2018/2/23 15:17:06 LI You-qiong, YANG Wen-hui, HUANG Hui-ping, et al. 688true ［Abstract］　Objective　To investigate indications and efficacy of the microsurgical resection for cerebral arteriovenous malformation(AVM) in the subthalamic section area.Methods　A retrospective analysis of the clinical data of the four patients with AVM in the subthalamic section area treated by microsurgical resection was performed. All cases had preoperative intracranial hemorrhage, Spetzler-Martin grade Ⅲ. All cases underwent preoperative cerebral angiography and 2 cases received partial embolization.Results　There was no operative deaths, neurological deficit had no aggravated. Following cerebral angiography vascular showed malformation mass completely disappeared.Conclusion　Microsurgical resection for appropriate cases with the subthalamic section area AVM can achieved better clinical outcome.]]> 2018/2/23 15:17:06 ZHONG Shu, ZHANG Hong-qi,LIANG You-ming, et al. 687true ［Abstract］　Objective　To investigate the influence of early intensive antihypertensive therapy combined with sub-hypothermia on recurrent hemorrhage and prognosis of the hypertension intracerebral hemorrhage(HICH) patients after minimally invasive surgery.Methods　One hundred and sixty acute HICH patients with blood pressure >180/105 mmHg, the course of <24 h, and executed minimally invasive surgery were chosen; then the patients were divided into early intensive antihypertensive therapy combined with sub-hypothermia group(group A, 80 cases) and standard guideline antihypertensive therapy group(group B,80 cases) randomly,and the situation in two groups was observed of recurrent hemorrhage and daily living ability after two months.Results　Recurrent hemorrhage was found 2 cases in group A, 10 cases in group B.There was statistical difference between the two groups on recurrent hemorrhage and daily living ability after two months (P<0.05).Conclusion　Early intensive antihypertensive therapy combined with sub-hypothermia can reduce the incidence of recurrent hemorrhage and improve the prognosis effectively, it is worthy of extending.]]> 2018/2/23 15:17:06 ZHONG You-an,XIAO Kai-min,ZHONG Liang,et al. 686true ［Abstract］　Objective　To monitor the epidemic subtypes and evolution of HIV-1 in cerebrospinal fluid fluid and plasma of infected chinese patients in Henan and Yunnan Povinces by analyzing env gene.Methods　The C2-V5 regions of the env gene was amplified by nested PCR following RNA reverse transcription extracted from both cerebrospinal fluid (CSF) and blood samples of HIV-1 positive patients. The amplicons were then sequenced，and the genetic subtype was identified by comparative analysis. The phylogenetic tree was constructed.Results　Sequence analysis revealed that the sequences from Yunan Province clustered closely with subtypes CRF07-BC, CRF08-BC, and C, and Henan with subtypes B and B’. Some CNS clonal sequences from Patient 1 of Henan Province fell on different branches and clustered closely with the sequences of Patient 5; The sequences from the CSF and blood in patients 12 and 127 from Yunnan Province were all monophyletic and fell on different branches, but in the other three patients sequences from the CSF and blood were intermingled with each other.The levels of diversity revealed significant difference between the CSF and plasma samples from Henan patients（P<0.05）.Conclusion　HIV-1 gene C subtype prevailed in Yunnan Province, while B and B’ subtypes prevailed in Henan Province. The sequences from blood showed a significantly higher divergence from those from the CSF.]]> 2018/3/12 9:42:55 LI Li，ZHAO Huan-ying, WU Yan-ning，et al. 685true ［Abstract］　Objective　To investigate the locating embedding methods of the miniature chicken embryo craniofacial tissue.Methods　On the base of routine paraffin section techniques, the specimen that was cleared and infiltrated was put into the embedding box, stuffing with paraffin. And the miniature chicken embryo craniofacial tissue was hold up with the microscopic tweezers, put it into L-shaped embedding mold rapidly according to the needed direction. Then the tweezers were removed when the paraffin starts to condense, and the embedding mold was filled with paraffin when it mainly coagulates until it completely solidified, then the paraffin block was removed.Results　This method made the embedding direction of the miniature chicken embryo craniofacial tissue turning out good, as well as good locating and displaying of each craniofacial prominence, and the specimen was more symmetrical and transparent.Conclusion　Embedded by this method, the miniature chicken embryo tissue craniofacial part has a good positioning. After the tissue sections are staining, its morphology can be clearly observed. The miniature chicken embryo craniofacial part can be used as an animal model to study its morphogenesis, which lays the foundation for the histological techniques.]]> 2018/3/12 9:42:55 WANG Shu-ting, CAO Yang, SUN Jin-hu, et al. 684true ［Abstract］　Objective　To Explore the endothelial function in obstructive sleep apnea hypopnea syndrome (OSAHS) patients with coronary atherosclerotic artery disease (CHD).Methods　One hundred and eighty-four subjects were recruited in our hospital from July 2009 to February 2013, recorded the gender, age, height, weight, etc,and all subjects attended sleep-laboratory polysomnography and coronary angiography.Fasting venous blood before coronary angiography was collected,and the level of plasma thrombomodulin(TM) was detected by the enzyme-linked immunosorbent assay(ELISA).Results　According to the results of polysomnography and coronary angiography, 184 patients were divided into control group, CHD group, OSAHS group, and OSAHS-CHD group. There were no significant difference among the four groups in age, sex, and body mass index (BMI). In plasma TM level, the control group were lower than CHD group,OSAHS group,and OSAHS-CHD group (2.91±1.81 VS 20.45±35.57,2.91±1.81 VS 21.93±44.32,2.91±1.81 VS 42.13±59.82,P all <0.05); OSAHS-CHD group was significantly higher than the control group, CHD group, OSAHS group(42.13±59.82 VS 2.91±1.81, 42.13±59.82 VS 20.45±35.57,42.13±59.82 VS 21.93±44.32，P<0.05), but no significant difference between CHD group and OSAHS group （20.45±35.57 VS 21.93±44.32，P>0.05）.Conclusion　Patients with OSAHS and CHD have a serious vascular endothelial function disorder, OSAHS is one of the risk factors for coronary heart disease.]]> 2018/3/12 9:42:55 YUAN Jun, JIANG Li-jun,LIN Ying-zhong,et al. 683true ［Abstract］　Objective　To study the change of levels of sICAM-1 and its relation with metabolic indicators, sex hormones in patients with PCOS, in order to explore the role of sICAM-1 levels in PCOS.Methods　Thirty-one patients with PCOS were selected randomly. Body mass index, waist-to-hip ratio, serum of sICAM- 1, sex hormones, lipids, fasting glucose, fasting insulin were detected before and after Diane-35 treatment of three cycles.Results　（1）Serum sICAM-1 was significantly decreased after treatment（P<0.01）.（2）FSH,LH,T, hair score were significantly decreased after treatment,while HDL,apoA,apoA1/B,PRL,Body mass index were significantly increased.（3） Levels of sICAM-1 in women with PCOS were correlated positively with BMI,TG,LDL,apoB, but correlated negatively with HDL.Conclusion　Serum sICAM-1is closely related with PCOS. Diane-35 could significantly improve the clinical symptoms of PCOS patients, and can reduce serum sICAM-1 levels.]]> 2018/3/12 9:42:55 XIAO Li, JIANG Feng-yan, QIN Yu-qin, et al. 682true ［Abstract］　Objective　To explore the relationship between glomerular filtration rate (GFR) and severity of coronary lesions in elderly patients.Methods　Two hundred and forty elderly patients were selected as research subjects, and according to the results of coronary angiography the coronary lesions integral and coronary stenosis integral were calculated. The modified abbreviated MDRD equation suitable for our country was used to estimate GFR (eGFR).According to eGFR of the study subjects were divided into normal renal function group, mild renal insufficiency group and moderate to severe renal insufficiency groups.The relationship between eGFR and coronary lesions integral or coronary stenosis integral were analyzed.Results　The age in mild renal insufficiency group and moderate to severe renal insufficiency group were higher than that in normal renal function group(P<0.05), and showed that with the growth of the age, the glomerular filtration rate declined.The proportion of males in moderate to severe renal insufficiency groups was higher than that in normal renal function group(P=0.002).But there was no significant difference between the normal renal function group and mild renal insufficiency group or between mild renal insufficiency group and moderate to severe renal insufficiency groups（all P>0.05）.The coronary lesions integral and coronary stenosis integral in the moderate to severe renal insufficiency group were greater than that in the normal renal function group（P<0.05）.eGFR was negative correlated with coronary lesions integral(r=-0.149,P=0.021) or coronary stenosis integral (r=-0.166,P=0.01).Conclusion　In elderly patients, there is negative correlation between eGFR and severity of coronary lesions.]]> 2018/3/12 9:42:55 HUANG Qian-ping,WU Yin-xiong,DENG Jin-long,et al. 681true ［Abstract］　Objective　To detect the expression of TGF-β1 in oral squamous cell carcinoma(OSCC) of human and mouse so as to investigate their role in the development of oral carcinoma.Methods　Immunohistochemical method was used to detect and analyse the expression of TGF-β1 gene in 12 cases of normal oral mucosa, 12 cases of oral dysplasia and 12 cases of oral squamous cell carcinoma in human and mouse, respectively.Results　In the group of normal oral mucosa of human and mouse, the positive TGF-β1 protein expression rates were 33.3%, 41.7 %, respectively; and in the group of oral dysplasia, the rates were 50.0%, 58.3 %, respectively; while in the group of OSCC, the rates were all 75.0%.Conclusion　As the degree of malignancy increased, the expression of TGF-β1 protein of human and mouse increased gradually and there was the similar expression of position and intensity. This suggests that TGF-β1 plays an essential role in tumor progression and the development of OSCC of the human and mouse may be similar to each other.]]> 2018/3/12 9:42:55 WEI Bao-sheng, YU Da-hai，LI Ming-wu,et al. 680true ［Abstract］　Objective　To observe the effect of ligustrazine on osteocyte apoptosis rate in rabbits,and to explore its protective effect.Methods　Forty-eight New Zealand white rabbits were chosen and randomly divided into four groups. The experimental group was administered the strong distilled spirits containing 50%(v/v) of alcohol at a dosage of 8 ml/（kg·d）. The experiment groups were divided into Saline control group, and high-,middle- and low-dosage ligustrazine ［8,4 and 2 mg/（kg·d） respectively］ groups. Moreover 12 New Zealand white rabbits as a normal control group received no treatment. The animals in five groups were sacrificed in batches 1, 2, 3 and 6 months after the procedures. The changes of histology in the Hepar and femoral head were observed. The osteocytes apoptosis was measured by means of TUNEL.Results　At 3, 6 months, compared with the control group the number of osteocyte apoptosis in the high-,middle-dosage ligustrazine group gradually reduced. The apoptosis rate of osteocyte differed from those in other groups(P<0.05). At six months,the number of osteocyte apoptosis in high-, middle-dosage ligustrazine groups were close to the number of osteocyte apoptosis in the control group(P>0.05).Conclusion　A certain range of dosage and course of ligustrazine has a protective effect on the apoptosis of osteocytes.]]> 2018/3/12 9:42:55 CAI Min,LI Hong-yu,LAI Wen-bin,et al. 679true ［Abstract］　Objective　To find,on the basis of laboratory research,a safe vertical movement and fixation method of micro-injection pump in order to meet the clinical needs and avoid the effect of temporary infusion rate change during movement on the patient’s vital signs.Methods　Sixty cardiac surgery patients who continued postoperative infusion of sodium nitroprusside by micro-injection pump were sent back to the ICU in the department of Cardiothoracic Surgery were observed,micro-injection pump was moved two times,each time of （15 ± 5）cm,until to achieve the height of （30 ± 5）cm at the bedside.The continuous monitoring of the heart rate and mean arterial blood pressures before movement and within 10 min after movement were recorded and compared.Results　The patient’s mean arterial blood pressure fluctuated in the range of 82.5 ～ 84mmHg before and after two movements.The differences were less than 1.5mmHg.The average heart rate fluctuated between 90.5 ～ 92.5 beats/min; the difference was 2 times/min,the fluctuation range was very small.No significant effects on the patient’s arterial pressure and heart rate were found.Conclusion　The safe staging vertical movement height of micro-injection pump with minimal temporary infusion rate change is≤（15 ± 5）cm.The purpose of the movements is to achieve a height of clinical requirement in fixed pump and did not cause the change of patient’s vital signs.This is a secure methods of moving and fixing micro-injection pump.]]> 2018/3/12 9:42:55 LUO Qiao-duan，MO Zhi-jiang,YAO Jin-zhen,et al. 678true ［Abstract］　Objective　To evaluate the value of multi-slice helical CT multiplaner reconstruction in the diagnosis of communicative tumors invading anterior skull base.Methods　The clinical data and CT image data of 21 cases of communicative tumors invading anterior skull base were respectively analyzed. All patients underwent multi-slice helical CT plain and enhancing scan, then all patients were made multi-plane reorganization(MPR).Results　On the basis of axial images of 64-slice CT combined with MPR for diagnosis of communicative tumors invading anterior skull base,there were 16 cases of cranio-nose neoplasms,4 cases of cranio-nose-orbital neoplasms and 1 case cranio-nose-orbital-oral neoplasms.Conclusion　The 64-slice CT have isotropic advantage. It has an importance value in clear positioning and quality of communicative tumors invading anterior skull base and provides a good anatomical structure and contributes to selecting the best path for clinical operation.]]> 2018/3/12 9:42:55 LIU Guang-jun，QIU Wei-jia，ZHOU Zhi-peng，et al. 677true ［Abstract］　Objective　To investigate the impacts of using different methods of ephedrine hydrochloride nasal drops prior to HBO therapy on patients’ tolerance of HBO therapy.Methods　Three hurdred patients firstly treated by HBO were selected and randomly divided into two groups, i.e. observed group and control group, 150 patients for each. In the 1st, 2nd and 3rd day, ephedrine hydrochloride nasal drops were put before they were sent into large medical hyperbaric oxygen tank. Patients in observed group laid in horizontal position with head leaning back and turning to 45°, and those in control group were in horizontal position with head leaning back only. three drops of 1% of ephedrine hydrochloride nasal drops were respectively put into the nose and then their responses were checked for comparison.Results　It was found that rate of swollen ear and earache, cases of HBO intolerance in observed group was obviously less than that in control group（93.3% VS 86.0%）, there was statistically significant between two groups(P<0.05).Conclusion　Ephedrine hydrochloride nasal drops put in horizontal position with head leaning back and turning to 45° before HBO is superior to that in the same positioncan with head leaning back only in dicrease and prevention of adverse reaction of ear. It is found that the patients’ tolerance to HBO obviously enhances.]]> 2018/3/12 9:42:55 KONG Lei, CHEN Shi-qiang, XU Li-min,et al. 676true ［Abstract］　Objective　To investigate the feasibility of applying storage autologous transfusion in liver cancer selective surgery.Methods　Thirty-four patients with liver cancer undergoing selective surgery adopted autologous blood storage and preparation, the changes of Hb and BP before and after blood collection were analyzed.Results　The maximum blood collection volume was 1200ml with the average of 726ml, Hb differences before and after blood collection were significant（P<0.05） and but were still within normal range. The BP differences before and after blood collection were not significant（P>0.05）, and surgical treatment could be selected according to anticipation without perioperative hepatic encephalopathy.Conclusion　Storage autologous blood transfusion is simple, safe, affordable, and worth to promote and use among patients undergoing liver cancer surgery.]]> 2018/3/12 9:42:55 CHEN Shao-long, ZHOU Shi-nan, HE Zhuang 675true ［Abstract］　Objective　To construct eukaryotic expression vector pcDNA₃-HBsAg-ROP₂ by amplifying HBsAg by PCR.Methods　According to the specific sequence of HBsAg and restriction sites of pcDNA₃-p30-ROP₂, the primers were designed and synthesized. HBsAg was amplified by PCR, then purified HBsAg gene was inserted into vector instead of p30 by enzyme digestion and ligation, and the construct was identified by enzyme digestion, PCR and sequencing.Results　0.7 kb of HBsAg was aquired, and the pcDNA₃-HBsAg-ROP₂ eukaryotic expression recombinant plasmid was constructed.The results of PCR and digestion showed that HBsAg and ROP₂ in the recombinant plasmid contained the complete genes sequences.Conclusion　The recombinant plasmid pcDNA₃-HBsAg-ROP₂ was successfully constructed.]]> 2018/3/12 9:43:40 WEI Qing-kuan, XIAO Ting，LI Jin,et al. 674true ［Abstract］　Objective　To study affection of nimotop on the Na⁺-K⁺-ATPase of neurons in ischemic penumbra in rats with acute cerebral ischemia-reperfusion injury.Methods　Four hundred and fifty male Wistar rats were randomly divided into three groups (control group, nimotop group and sham operation group); the 3 groups were further divided into 6 h, 24 h, 48 h, 72 h and 7 d group according to the time of reperfusion. The rats model of ischemia reperfusion (2 h) were induced by thread approach. Only the nimotop group received medical interventions. The rats were executed at reperfusion 6 h, 24 h, 48 h, 72 h, 7 d to observe the neurological symptoms grade, the activities of Na⁺-K⁺-ATPase, the changes of brain water content and infarction size.Results　The comparison of each observation index of the three groups and different time points reached statistical significance (P<0.01). At 24 h,48 h and 72 h point, the activities of Na⁺-K⁺-ATPase and the brain water content in nimotop group were significant difference than in control group (P<0.05).while, at 24 h,48 h,72 h and 7 d, the infarction size and neurological symptoms grade in nimotop group were significantly lower than control group(P<0.05). The activities of Na⁺-K⁺-ATPase were down-regulated in control group, beginning at 6 h, bottoming at 48 h, up at 72 h and steadying at 7 d.Conclusion　Nimotop have protective effect on neurons in ischemic penumbra in rats with acute cerebral ischemia-reperfusion injury, the mechanisms is related not only to the inhibition of calcium overload, but also to improving energy metabolism.]]> 2018/3/12 9:43:40 TANG Shi-ting, LI Lü-li, ZHANG Li-xiang, et al. 673true ［Abstract］　Objective　To investigate the clinical efficacy of the treatment of intraventricular hemorrhage by external ventricular drainage combined with the Ommaya reservoir implantation.Methods　Forty-one patients with intraventricular hemorrhage were treated by traditional ventricle external drainage in one side ventricle and external ventricular drainage combined with Ommaya reservoir implantation in other side ventricle. The Ommaya reservoir was implanted under the scalp by percutaneous puncture.Results　Accoring to GOS prognostic criteria, good recovery was found in 24 patients, moderate disability in 8 patients,severe disability in 4 patients，long-term vegatative status in 2 patients,3 patients were dead.No intracranial infection was found.Conclusion　The external ventricular drainage combined with Ommaya reservoir implantation can effectively reduce the incidences of intracranial infection and hydrocephalus, and improve the therapeutic effects of intraventricular hemorrhage.]]> 2018/3/12 9:43:40 HUANG Bin,YANG Lei,LIU Kai-sheng,et al. 672true ［Abstract］　Objective　To investigate the 90 days follow-up result of mRS after the treatment of Urinary kallidinogenase in the patients with progressive cerebral infarction in different imageology styles.Methods　Two hundred and seventy-seven patients with progressive cerebral infarction from March 2007 to June 2012 were divided into four subgroups according to different imageology styles:large infarct,middle infarct,small infarct and lacunar infarct.These patients were randomly divided into two groups:treatment group with urinary kallidinogenase(n=141) and control group(n=136).Two groups were administrated intravenous injection of Shuxuetong 6 ml in physiological saline 250 ml, Citicoline 0.5 g in physiological saline 250 ml and oral aspirin 0.1 g per day for 4 weeks.On the basis of above treatment,treatment group was administrated intravenous infusion of urinary kallidinogenase 0.15 PNAu in physiological saline 100 ml,control group was administrated physiological saline 100 ml per day for 7～14 days.The efficacy was evaluated by mRS(Modified Rankin Scale) at the end of treatment and 90 days after-discharge respectively.Results　The resuts showed that 90 days affter-discharge,the scores of mRS in two groups decreased, especially in large infarct,middle infarct and small infarct subgroups.The treatment group was superior to control group in decrease of the scores of mRs （P<0.05）.Conclusion　Urinary kallidinogenase is effective to promote long-term neurofunctional recovery and step down mutilation of progressive cerebral infarction.]]> 2018/3/12 9:43:40 WEN De-shu, MA Li-qin, WU Zhi-wu,et al. 671true ［Abstract］　Objective　To explore the effects of individual cardiac rehabilitation exercise on left ventricular hypertrophy of patients with hypertension in rural communities.Methods　Eleven hundred patients with hypertension and left ventricular hypertrophy were randomly assigned to exercise group(589 cases) and control group(511 cases). After removal of patients who were missed visit or did not complete exercise prescription the actual number of exercise group were 467 cases; the that of control group were 468 cases. The exercise group received the developed individual exercise prescription and strictly completed its whole process.The control group received general guidance on rehabilitation exercise. Before and after 6 months of exercise two group received echocardiographic examination, blood lipid, blood glucose and liver and kidney function tests, and their curative effects were analyzed.Results　In the exercise group,there were statistically significant difference in the indexes of left ventricular hypertrophy before and after exercise (P all <0.01).There were statistically significant in the indexes of left ventricular hypertrophy after exercise between two groups (P all <0.01).In exercise group there were statistically significant difference in blood glucose, blood lipid(P all <0.01),and was no statistically significant difference in liver and renal function before and after exercise(P>0.05).In control group,there were no statistically significant difference in bood glucose,blood fat, liver and kidney function before and after exercise(P>0.05).Conclusion　Personal cardiac rehabilitation exercise could fully reverse or mitigate LVH in the part of hypertensive patients, some patients can reduce or stop use of antihypertensive drugs.It has a function of adjusting blood fat and blood glucose with less adverse reaction.]]> 2018/3/12 9:43:40 ZHAO Ying-yi，JIANG xi，HE Weng-zhen，et al. 670true ［Abstract］　Objective　To understand the hypertension management effect and the differences between city and rural areas under the national basic public health services, and probe into the related countermeasures.Methods　Four hundred and 300 hypertensives respectively from Nanning qingxiu district (city group) and Hengxian (rural group) were chosen by simple random sampling, among those attending the basic public health services for at least 1 year. The differences treatment and control rate between city and rural were tested using χ² tests.Results　The total hypertension treatment rate was 68.86%, city patients’ treatment rate was 83.25%, much higher than 49.67% of rural patients (P<0.01). High blood pressure control rate was 56.29%, city patients’ was 70.25%, much higher than 37.67% of rural patients (P<0.01).Conclusion　The state’s basic public health services hypertension management can improve hypertension treatment rate and control rate dramaticly, but the effect in rural areas was worse than in city. Corresponding effective measures should be taken to improve the hypertension treatment and control rate in rural areas.]]> 2018/3/12 9:43:40 ZHOU Fang-hua, ZHOU Ji, SHI Jian, et al. 669true ［Abstract］　Objective　To investigate single nucleotide polymorhtism of methylenetetrahydrofolate(MTHFR) reductase gene and its association with the side effect of methotrexate in the treatment of rheumatoid arthritis(RA).Methods　The clinical and laboratory data of 183 patients with RA were evaluated before treatment and at 0,12,24,48 weeks after treatment.Efficacy and side effects of medication were also analyzed.Real-time fluorescent quantitative PCR was conducted to test gene mutations in RA patients and 100 healthy controls.Results　There was no significant difference in the frenquency of 6777CC,CT,TT between RA patients and the healthy controls（P>0.05）.There was no significant difference in the frenquency of 6777CC,CT,TT between the group in which MTX was effective and the other groups in which MTX was not effective（P>0.05）.There was significant difference in the frenquency of 6777CC,CT,TT between the group with MTX related adverse effects and group without MTX related adverse effects.The occurrence rate of side effects of MTX in the patients with 677TT was higher than those without mutation(CC)(P<0.05).Conclusion　There is no relationship between the pathogenesis of RA and the C677T mutation of MTHFR gene.MTHFR gene C677T mutation is probably one of genetic risk factors for the sidde effects of MTX,such as hepatic toxicity and adverse reactions of the blood system.]]> 2018/3/12 9:43:40 SHI Yu-hong,ZHOU Run-hua,XU Jia,et al. 668true ［Abstract］　Objective　To investigate the effect of Cdx2 gene silencing of recombinant lentivirus particles (Cdx2-RNAi-LV ) on expression of Survivin and C-myc in human gastric cancer SGC-7901/DDP resistant cells transplanted subcutaneously in nude mice.Methods　The subcutaneous transplantated tumor models in nude mice were established with cell line SGC-7901/DDP of human gastric cancer. The 36 of model mice were randomly divided into 3 groups(n=12): PBS group, LV-RNAi group, Cdx2-RNAi-LV group, each group of tumors were respectively injected with phosphate buffer,the lentivirus particles (0.5×10⁸ TU/ml), containing Cdx2 gene recombinant lentiviral particles (0.5×10⁸ TU/ml), the corresponding volume of drugs which were all equivalent 100 μl, once every three days for six times.And all mice were intraperitoneally injected with the drug cisplatin （25 mg/kg）,once every two days for ten times.After 20 days, all mice were sacrificed and subcutaneous tumors were taken out. Survivin and C-myc mRNA and protein were detected by semi-quantitative reverse transcription polymerase chain reaction （RT-PCR）and Western blot.Results　Among groups of Cdx2-RNAi-LV,LV-RNAi and PBS,the mRNA and protein of Survivin and C-myc were all expressed in cells, but the corresponding expression levels of mRNA and protein in each group cells were not all the same. Compared with LV-RNAi group and PBS group, Survivin and C-myc mRNA and protein were downregulated in Cdx2-RNAi-LV group(P<0.05). However,no significant difference was found between LV-RNAi group and PBS group(P>0.05).Conclusion　The Cdx2 Gene Silencing of recombinant lentivirus particles intratumorally injected could significantly downregulate the expression of Survivin and C-myc in gastric carcinoma transplanted tumor carried by nude mice, and the down-regulation of Survivin and C-myc expression perhaps is one of the Cdx2 gene silencing reversal of tumor resistance mechanisms.]]> 2018/3/21 8:06:38 WEI Wei-yuan,KONG Fan-biao,WANG Xiao-tong,et al. 667true ［Abstract］　Objective　To analyze the reasons and value of long-term survival of the patients after heart transplantation.Methods　From Oct 2004 to Dec 2012, 34 patients( 29 males, 5 females, age 12～56 years) underwent bicaval orthotopic cardiac transplantation in Guangxi Zhuang Autonomous Region People’s Hospital and 181 Hospital of P.L.A. These patients included 30 cases of dilated cardiomyopathy, 2 hypertrophic cardiomypathy and 2 coronary artery disease. Cardiac functions were grade Ⅲ～Ⅳ.The analysed factors included individual factors, operative procedures,perioperative period status,immunosuppressive therapy and detection,and life quality.The follow-up period were 1～99 months.Results　Early death were 3 cases and 2 patients died of pulmonary hypertension crisis-right ventricular function failure.All 31 discharged patients survived, their cardiac functions restored to grade Ⅰ～Ⅱ, the follow-up period of 13 cases were over 3 years, follow-up period of 1 case was over 8 years(this patient had given birth to a child). No abnormal reaction was observed by immunosuppressive concentration detection and B-ultrasonography examination. No stenosis of coronary artey was observed by 64-slice spiral CT.Conclusion　The main factors long-term survival include HLA typing sera, ischemia reperfusion injury,and fair use of immunosuppressive medicine. Preventing immunologic rejection and infection are important measures to prevent heart transplant angiopathy. Noninvasive examinations such as 64-slice spiral CT could become common monitoring mearures.]]> 2018/3/21 8:06:38 LIN Hui, PAN Yu-chen, WEN Zhao-ke,et al. 666true ［Abstract］　Objective　To validate the portable recording device（Apnealink） for use as a screening tool for obstructive sleep apnea-hypopnea syndrome in clinical practice in the south of china.Methods　We compared the AHI obtained from the Apnealink device during a study in the subjects’ homes or in the laboratory to that obtained during attended sleep-laboratory polysomnography to assess the sensitivity，specificity，false positive rate，false negative rate and kappa value of the device in consecutive subjects. The portable sleep monitor study was was performed in the next day of the sleep-laboratory polysomnography study. Using receiver operating characteristic (ROC) curve,we obtained the best cut-off point of Apnealink.Results　When obstructive sleep apnea-hypopnea syndrome was diagnosed on a cut-off of an AHI value of 5 events per hour, the Apnealink device had a sensitivity of 87.9%, a specificity of 95.0%,a false negative rate of 12.1%,a false positive rate of 5.0% and a kappa value of 0.805（P=0.000）. Using ROC curve,we obtained a best cut-off point of Apnealink of AHI value of 5 events per hour(sensitivity 87.9%,specificity 95%).The area under the curve (AUC) of ROC curve was 97.7%(95% confidence interval 0.946 to 1.007,P=0.000).Conclusion　This study demonstrates that the Apnealink device is highly sensitive and specific in calculating AHI,and is useful in the diagnosis of OSHAS. It is suggested to use a AHI value of 5 events per hour as a best cut-off point of Apnealink.]]> 2018/3/21 8:06:39 JIANG Li-jun, LIN Ying-zhong, LIU Jian-hong,et al. 665true ［Abstract］　Objective　To observe the long-term toxicity of Ruanjian Hugan Tablet(RJHGT) in SD rattus norregicus.Methods　One hundred and sixty rattus norregicus were randomly divided into four groups (40 in each group);low,moderate,high dosage groups(groupL,M,H) and control group(group C).Experimental groups were given RJHGT by garage,and group C was given distilled water instead. The experiment was carried out for 26 consective weeks.General conditions,body weight,food intake of SD rattus norregicus were observed.Hematology,biochemical parameters,coagulation function, organ coefficient,histopathology changes of SD rattus norregicus were detected in 13 and 26 weeks of the experiment,and in 4 weeks after drug withdrawal.Results　All the indexes mentioned above were normal in group L,M and H.Conclusion　The toxicity of RJHGP is low when it is used in its clinical range,and it is safe.]]> 2018/3/21 8:06:39 WANG Yong-ling, KONG Xiao-long, MO Feng-zhen,et al. 664true ［Abstract］　Objective　To investigate the association between Human Leukocyte antigen DR(HLA-DR) gene polymorphisms and asthma in children.Methods　Eighty-four unrelated asthmatic children and one hundred and sixty-eight healthy controls without asthma and atopy were involved in the study. All asthmatics had their serum total IgE levels measured with UniCAP Pharmacia system were taken among the asthmatic children. HLA oligonucleotide array was used to detect twenty-one gene frequencies of HLA-DR, and massARRAY system (Sequenom) by means of matrix assisted laser desorption ionisation-time of flight mass spectrometry method(MALDI-TOF) for HLA-G gene single nucleotide poly -morphism (SNP) genotyping.Results　(1) The frequencies of HLA-DRB1_*070X allele and HLA-DRB1_*11XX allele in the asthmatic childern were 2.98% and 13.69%, significantly higher than those in healthy controls (0.3% and 5.95%， χ²=6.915，P<0.05; χ²=9.478，P<0.01), Odds ratios (Ors) were 10.57（95%CI: 1.215～91.986） and 2.79（95%CI: 1.429～5.449） respectively. HLA-DRB3(52)_*010X allele were significantly decreased in asthmatics compared to healthy controls (13.99%, χ²=5.854，P<0.05)，Ors were 0.429（95%CI:0.214～0.869）.(2)The individuals with HLA-DRB1_*15XX-rs1704/rs 1063320 locus and 14-bp deletion/G haplotype was lower asthmatics risk than those without the same haplotype, and Ors was 0.198（95%CI:0.084～0.471), also the level of serum value of IgE in asthmatic children were significantly lower than those without the haplotype of rs1704/rs1063320 14-bp deletion/G(P < 0.05).Conclusion　HLA-DRB1_*070X allele and HLA-DRB1_*11XX allele were implicated in susceptibility to asthma, HLA-DRB3(52)_*010X allele might confer protection against asthma. And the haplotype of HLA-DRB1_*15XX- rs1704/rs1063320 14-bp deletion/G are protective from children asthma.]]> 2018/3/21 8:06:39 XIE Qing-ling, ZHEN Hong, QIN Lin, et al. 663true ［Abstract］　Objective　To investigate the clinical manifestations, clinical imaging features and related risk factors of 104 massive cerebral infarction patients, and to provide the basis for the prevention of large area cerebral infarction.Methods　By reviewing the records of 104 massive cerebral infarction patients who had been performed ECG, echocardiogram, computetized tomography, magnetic resonance imaging or angiography, transcranial Doppler, and Carotid color Doppler ultrasound evaluation,both the site of infarctions and the causes of vascular occlusion were investigated.Results　Among the 104 massive cerebral infarction patients, 69 patients(66.3%) had cardiogenic cerebral embolism, including 39 cases of coronary heart disease, 16 cases of old myocardial infarction, 7 cases of rheumatic heart disease, 3 cases of dilated cardiomyopathy, 2 cases of hyperthyroid heart disease, and 1 case of myocardial noncompaction. 63(60.6%) patients had atrial fibrillation,and 75 patients(72.1%) had hypertension.Conclusion　Both atrial fibrillation and cardiogenic cerebral infarction are the most important cause of massive cerebral infarction, and artery-artery embolization is its another important cause on the basis of the artery stenosis.]]> 2018/3/21 8:06:39 SONG Cheng-fu,Wang Jia-jun,FENG Jian-yu,et al. 662true ［Abstract］　Objective　To investigate the conditions of perioperative application of antibiotics in 3 kinds of clean incision operation and the effect of intervention measures in our hospital.Methods　The conditions of drug uses in 3 kinds of clean incision operation (thyroidectomy，mastectomy or hernia orrhaphy) were investigated and analyzed in our hospital in Oct.2009，Apr.2010，Oct.2010，Jun.2011.Results　After intervention measures, there was not unreasonable phenomenon in the drug selection，one dose, delivery opportunity，delivery road, intraoperative additions,and so on. There were remarkable improvements in the delivery time. However，there were no great improvements in medication of no indications.Conclusion　A series of intervention measures are effective in the application of antibiotics in our hospital，and they provide an effective and feasible model for promoting rational use of drugs.But it is far away from the demand of Ministry of Health.It is necessary to strengthen the management of antibiotics application.]]> 2018/3/21 8:06:39 LIAO Yi, CHEN Ying 661true ［Abstract］　Objective　To investigate the specific anti-leukemia cell effect of cytotoxic T lymphocytes(CTL) induced by acute myeloid leukemia-derived dendritic cells(AML-DC).Methods　Acute myeloid leukemic cells were induced to differentiate into AML-DC.T cells from umbilical cord blood were activated with the cytokine combined with AML-DC. T cells were identified by flow cytometer(FCM). The anti-tumor activity of T cells was assayed with LDH method.Results　Highly reactive anti-AML T cells were generated and expanded. The CD3⁺ T cells rate was (79.7±3.7)% in the group after stimulation by AML-DCs. These T cells caused specific lysis of (48.35±12.75)% target AML cells. And the difference was significant compared with T cells without stimulation of AML-DCs(P<0.05).Conclusion　Leukemic cells of patients with acute myeloid leukemia could be differentiated to DC-like cells possessing the ability of stimulating anti-leukemic immune response.]]> 2018/3/21 8:07:41 WANG Cai-xia, ZHANG Yu-ping, WANG Shun-qing,et al. 660true ［Abstract］　Objective　To explore the change of JNK (Thr¹⁸³/Tyr¹⁸⁵) activity in the peripheral blood mononuclear cells in the newly diagnosed type 2 diabetes patients.Methods　A total of 60 healthy volunteers with normal glucose tolerance (NGT group), 60 newly diagnosed type 2 diabetes patients (NT2DM group) were recruited in our study. JNK (Thr¹⁸³/Tyr¹⁸⁵) phosphorylation levels were delected in the peripheral blood mononuclear cells by western blot method.Results　JNK (Thr¹⁸³/Tyr¹⁸⁵) phosphorylation levels in NT2DM group were significantly higher than that in NGT group(P<0.01). The correlation analysis showed that JNK (Thr¹⁸³/Tyr¹⁸⁵) phosphorylation levels were positively associated with body mass index, fasting blood glucose and low-density cholesterol (P<0.05). Under the situation of excluding the related factors such age, gender and so on, JNK (Thr¹⁸³/Tyr¹⁸⁵) phosphorylation levels were still independently associated with fasting blood glucose (P<0.05).Conclusion　JNK pathway over-activation may increase the risk of diabetes.]]> 2018/3/21 8:07:41 LIU De-ying,LU Wen-sheng 659true ［Abstract］　Objective　To evaluate the artifact sizes of three magnetic resonance imaging (MRI) sequences caused by different kinds of metallic dental materials.Methods　Cubical samples of dental casting alloy (Ag-Pd alloy, Au-Pd alloy, gold alloy, Ni-Cr alloy, Co-Cr alloy, titanium alloy and pure titanium) were placed in a cylindrical plastic vessel filled with distilled water. Ceramic pieces were used as control. Images were performed by means of 1.5 T MRI apparatus with 3 different sequences commonly used in brain MRI sequences.MRI artifact area of different metal materials and different sequences was calculated and compared.Results　ANOVA of factorial design indicated significant differences among different materials (F=27.920, P<0.001) and 3 sequences (F=52.771, P<0.001). Compared to control material the precious alloys produced the least artifact while titanium alloy, pure titanium the middle level and Ni-Cr alloy, Co-Cr alloy the largest. Among the 3 imaging sequences, GRET2 produced the largest artifact.Conclusion　Selection of specific metal material and imaging sequence can minimize the metal artifacts in MRI.]]> 2018/3/21 8:07:41 HAO Nan， PAN Xiao-bo，HAN Wu,et al. 658true ［Abstract］　Objective　To study the effect of mobile c-arm image mosaic technigue in clinical application of minimally invasive orthopedic surgery.Methods　Forty-three patients with complex fractures of lower extremity were treated by minimally invasive surgery,During operation lower extremity was examined by c-arm machine and 2～4 images were collected.And by image mosaic technigue apanoramic image on internal fixation site after reduction of fracture was obtained and evaluated;After surgery DR/CR radiography examination were performed,and the related angles wer measured.Results　After operation 43 patients were followed up for 6～16 a months,with an average 8 months.At the end of operation and at three mouths after operation, the review of X-ray photographs showed the good aligment and contraposition of fractures were achieved and all fractures healing were good.No plate fracture and screws loose was found.The evaluation of the mosaic image showed: Of 43 patients,excellent effect was found in 27 patients,good in 15 patients,fair in one patients,the excellent and good rate was 97.67%. In 43 patients,all angles measurements were within normal range.In comparison of the complate mosaic image with DR and CR images there was no statistically significant difference(P>0.05).Conclusion　The mobile c-arm mosaic panoramic imaging technique can timely and effectively meet the needs of orthopedic doctor for the intraoperative panoramic image,on the parts of reconstruction of femoral and tibial fractures,avoide the incorrect judgment on the whole effect of fracture reduction,the inapporpriate internal fixation,and provides a powerful support and guarantee for further improving the quality of minimally invasive orthopaedics surgery.]]> 2018/3/21 8:07:41 XIE Jia-bing,DING Guo-zheng,XU Zhu-jun,et al. 657true ［Abstract］　Objective　To improve the healing process after treatment on cervical lesion.Methods　Three hundred patients with single cervicitis and normal cytological examination results were collected between May 2009 and May 2010. Another 200 patients were confirmed to be cervical intraepithelial neoplasia (CIN)Ⅰ～Ⅱ by cervical cytology, HPV-DNA- colposcopy- Cervical biopsy. They were randomized divided into experimental group(routine treatment+Chitosan cervical antimicrobial films treatment) and control group with routine treatment only. The amount and duration of vaginal bleeding, duration of fluids from vagina and duration of healing of the wound were observed and analyzed. The patients with single cervicitis were treated with laser and those with CINⅠ～Ⅱ were treated with loop electrosurgical excision procedure (LEEP).Results　Compared with control group,the vaginal bleeding obvious reduced(P<0.01). The durations of vaginal bleeding, fluids from virgin and healing of the wound were significantly shortened after laser in the experimental group. The was statistically difference (P<0.01). The virginal bleeding in the experimental group was significantly less than those of the control group after LEEP(P<0.01) The durations of vaginal bleeding, fluids from vagina and healing of the wound in the experimental group were significantly shorter than those in the control group after LEEP. The was statistically difference(P<0.01).Conclusion　Physiotherapy combined with Chitosan cervical antimicrobial films can significantly reduce virginal bleeding and shorten the vaginal bleeding, fluids from virgin and healing of the wound without complications, which relieves pains and improve life quality of the patients.]]> 2018/3/21 8:07:41 ZENG Ding-yuan,QIN Mei 656true ［Abstract］　Objective　To prove the possibility that ovariectomized(OVX) and subsequently olfactory bulbectomized(OBX) female rat pattern(OVX+OBX) first established by Stock et al in year 2000 is able to become a feasible animal model of agitated depression in menopausal women.Methods　Fifty Sprague-Dawley adult female rats were randomly divided into five groups with ten rats per group:model，model control，model treatment，sham and intact.OVX and OBX were orderly performed on the first three group rats.Combined administration of fluoxetine（10 mg/kg,i.p.） and estradiol （100 μg/kg, s.c.） was given to rats in model treatment group after fourteen days of OBX，while saline solution was administered to rats in model control group，and the above daily interventions were conducted for a period of twenty-one consecutive days; while the remaining group rats were not treated at all. Open field test，forced swimming test and Morris water maze were utilized to detect behavioral changes of rats in all groups.Results　Rats in model or model control group showed early-onset and severe irritation，agitation，active attacks and fights or biting with each other. Meanwhile， the crossing score, rearing score, immobility time and escape latency in the above two group rats were the highest or the longest ，while the time rat swimming in the quadrant originally put a platform and the number of rat swimming through a fixed position for putting the platform became the shortest or the smallest. All these changes had a statistically significant difference in comparison with those in sham or intact group rats（P<0.01）. However，changes of the above parameters in model treatment rats became just opposite to those in model or model control group rats with a statistically significant difference（P<0.01）, and were also near the levels of related parameters in sham group or intact group rats with no statistically significant differences（P>0.05）.Conclusion　Our study can fully replicates the results， including OVX+OBX female rat model designed by Stock et al, and the behavioral changes，surgical causes and antidepressant treatment efficacies showed from OVX+OBX rat model in this study, which are very similar to the correspondent results clinically found in an agitated depression of menopausal women, and also share relative surface validity, construct validity and predictive validity, thereby indicating that the female rat OVX+OBX operational pattern has a strong potential to become a feasible agitated depression animal model of menopausal female patients.]]> 2018/1/24 8:18:04 MO Fei-rong，GUO Ling 655true ［Abstract］　Objective　To compare the difference in aesthetic indicators of facial profile between the patients with skeletal Class Ⅱ and Ⅰ malocclusion before and after the extraction treatment.Methods　The samples consist of 53 patients(27 with skeletal Class Ⅱ division Ⅰ malocclusion,and 26 with skeletal Class Ⅰ bimaxillary protrusion). Every patient were corrected by straight wire appliance and each of them were extracted four premolars. Lateral cephalograms taken before and after the treatment were evaluated to determine the difference in aesthetic indicators of facial profile.Results　The patients with class Ⅱ malocclusion showed significant changes in the angle of inclination of upper lip（A′Ls-FH）, the angle of inclination of chin（Pg′B′-FH）, Z angle and the angle of protrusion of profile(G-Sn-Pg′)after the treatment(P<0.01). The patients with class Ⅰ showed significant changes in A′Ls-FH, Pg′B′-FH and Z angle after the treatment(P<0.01). There was significant difference before and after treatment in Z angle and G-Sn-Pg′ between the two groups. But there was no significant changes in NsPg′-FH either intra- group or inter- groups before and after treatment(P>0.05).Conclusion　The facial profile of patients with skeletal Class Ⅱ and Ⅰ malocclusion can be affected by the extraction treatment. The main difference in profile between the two groups occurred in the region of soft chin. NsPg′-FH is not a sensitive indicator to determine the profile changes for the patients with skeletal Class Ⅱ and Ⅰ malocclusion.]]> 2018/1/24 8:18:04 FANG Zhi-xin, ZHOU Yan, ZHOU Jing, et al. 654true ［Abstract］　Objective　To explore the diagnostic significance of the Heidelberg retina tomograph(HRT-Ⅱ) in primary chronic angle-closure glaucoma(CPACG).Methods　HRT-Ⅱ and automatic perimeter(Humphrey detection) were used in 40 cases(80 eyes) of CPACG for testing optic disk and visual field, and the correlation between the optic disk parameters and average defect of visual field were analyzed, the statistical analysis of HRT-Ⅱ detection sensitivity was performed.Results　Seventy-four eyes were abnormal by HRT-Ⅱ detection, and 72 eyes were abnormal by Humphrey detection, the coincidence rate between Humphrey detection and HRT-Ⅱ detection was 97.30%. In 8 eyes which were normal visual field in Humphrey detection, there were 2 eyes abnormal and 6 eyes were normal in HRT-Ⅱ detection. In 6 eyes which were normal in HRT-Ⅱ detection, there were 6 eyes were normal visual field in Hunphrey detection. The average visual field detects(MD) showed a statistically significant positive with rim area(RA), rim volume(RV), optic cup shape measurement(CSM) and a negative correlation with cup dish area ratio(CDAR).Conclusion　HRT-Ⅱ detection has a high sensitivity and a better correlation with Humphrey detection, and can be used for monitoring and early diagnosis of optic nerve damage in patients with CPACG.]]> 2018/1/24 8:18:04 ZHONG Shan, LI Li 653true ［Abstract］　Objective　To screen effective medicine with VEGFR2 inhibitory activity from 8 novel VEGFR2 targeted inhibitors and to determine their anti-tumor properties.Methods　Two cell lines(Tca8113 cells from tongue cancer and HUVEC) were cultured respectively in groups with 6 different concentrations of the eight inhibitors(marked No.1 to No.8). Positive control using different concentrations of 5-FU and blank control without medicine were set up simultaneously. Cell growth was measured by MTT assay. Immunocytochemistry was carried out to determined VEGFR2 levels in groups with different saturated inhibitors or in blank control.Results　Cell growths of both cell lines were significantly inhibited in positive control groups comparing to that in blank control(P<0.05);there was no significant difference in cell growth between VEGFR2 inhibitor groups and blank control in the same cell line(P>0.05). Immunohistochemistry showed that VEGFR2 was highly expressed on the cell membranes of Tca8113 cells and HUVEC; comparing to blank control，only No. 6 VEGFR2 targeted inhibitor decreased VEGFR2 expression on Tca8113 cells significantly(P=0.000), while all inhibitors showed no VEGFR2 inhibition on either cell line(P>0.05).Conclusion　These VEGFR2 targeted inhibitors have no proliferative inhibition on the cell lines of HUVEC and Tca8113. However, No.6 inhibitor decreases the VEGFR2 expression on Tca8113 cells significantly thus becoming a candidate for further study.]]> 2018/1/24 8:18:04 LU Shu-jian, YU Da-hai, YAN Hong，et al. 652true ［Abstract］　Objective　To investigate the distribution of RhD and ABO blood type and gene frequencies in Zhuang nationality in Nanning, Guangxi in order to provide the evidence for making reasonable blood management plan and prevention of ABO hemolytic disease of newborn.Methods　The ABO and RhD blood groups of 4 527 unrelated individuals were analyzed by randomized sampling.Results　Blood type distribution showed O>B>A>AB,the genetic frequency included p(A)=0.1312, q(B)=0.1761 and r(O)=0.6927.The distribution of ABO blood type and genetic frequency met Hardy-Weinberg. Nationality index was 0.7636.The rate of positive RhD was 99.8012%,the rate of negative Rh(D) was 0.1988% and the rate of d gene was 0.044587.Conclusion　Zhuang nationality in Nanning had low rate of negative Rh(D) and distribution of ABO was characteristic of minority in this area, i.e. rate of O gene was high and rate of A gene was low.]]> 2018/1/24 8:18:04 LI Hai-lan,JIAO Wei,MO Zhu-ning, et al. 651true ［Abstract］　Objective　To explore the processing measures for cerebrospinal fluid leakage during spinal surgery and their curative effects.Methods　From January 2008 to December 2012， 30 patients of cerebrospinal fluid leakage during spinal surgery in our department,were chosen and randomly divided into two groups.Of them,15 patients were treated by the modified EC glue combined with fascia lata sealing(group A), and other 15 patients by the modified silk suture direct suture, gelatin sponge, fascia sealing(group B). Fifteen patients without dwral injury in the same period during spinal surgery were chosen as control group. Among three groups the incidence of postoperative complications, postoperative drainage volume and time were compared.Results　The incidence of postoperative complications, postoperative drainage volume and time in group A were lower or less than those in group B(P<0.05).The difference were statistically significant between two groups.Conclusion　EC glue combined with fascia lata sealing has good curative effect,and is a effective measure for preventing cerebrospinal fluid leakage after spinal surgery operation.]]> 2018/1/24 8:18:04 WEI Min-ke,YIN Dong,LIANG Bin,et al. 650true ［Abstract］　Objective　To observe the clinical effect of frozen jiaweisanhuangtang in the prevention of acute radiation-induced neck skin damage in patients with nasopharyngeal carcinoma.Methods　Thirty-one patients with nasopharyngeal carcinoma undergoing radiotherapy were enrolled in the study, frozen jiaweisanhuangtang were applied to right-neck as treatment group, and left-neck were as a blank control group.Results　The time of occurrence of skin injury at right-neck was (15.26±6.15)days, and the time at left-neck was (11.14±5.28)days, the difference was statistically significant(P<0.05). The incidence of skin damage in treatment group and control group were 74.19%(23/31) and 87.10%(27/31) respectively, the difference was not statistically significant(P>0.05); thus difference of degree of skin damage between two groups was statistically significant(P<0.05). The incidence of moderate to severe pain in treatment group and control group were 35.48% and 70.97% respectively, the difference was statistically significant(P<0.05). Thirteen point zero four percent of patients in treatment group showed skin damage before total 20 Gy of irradiation, and 59.26%(16/27) of patients showed in the control group, the difference was statistically significant(P<0.05).Conclusion　The frozen jiaweisanhuangtang can effectively prevent the occurrence of acute radiation-induced neck skin damage, especially reduce the degree of skin damage, and it is worth further study.]]> 2018/1/24 8:18:04 ZHANG Zheng,WENG Jing-jin, YANG Jia-zhen, et al. 649true ［Abstract］　Objective　To investigate the epidemiological characteristics and prevalent trend of AIDS among pregnant women in Nanning City, and to provide scientific basis for preventing AIDS.Methods　The preventing Mother-to-Child Transmission(PMTCT) report forms and HIV infections information from 2009 to 2011 were collected and analyzed.Results　Three hundred and seventeen pregnant women in Nanning City were infected with HIV. From 2009 to 2011, the HIV infection rate were 0.100%, 0.096% and 0.112%, respectively, which had no significant association among the three groups(χ²=1.572，P=0.456).Most of the HIV infected pregnant women aged from 20～34,highly to be 86.1% of the cases.Their nationalities were mainly Han nationality（156 cases/49.2%） and Zhuang nationality（143 cases/45.1%）,with an occupation as unemployed（106 cases/33.4%） and farmers（112 cases/35.3%）,as well as a poor education background for only received junior middle school or even under education（239 cases/75.4%）.Two hundred and twenty-seven cases were infected by sex transmission, accounted for 71.6%. From 2009 to 2011, the sex transmission rate of HIV infections were 0.061%，0.071%，0.087%, respectively. There was no significant association of the three groups(χ²=4.927，P=0.085). During 2009 to 2011,the termination of pregnancy rate of HIV infected pregnant women were 4.49%， 8.08%， 17.83%, separately,which had significant association among the groups（χ²=10.819，P=0.004）. Two hundred and ninety cases were implemented PMTCT,the blocking rates was 91.48%,and subannual blocking rates were 93.26%,90.91%,90.70%, which had no significant association （χ²=0.504， P=0.777）.Conclusion　The prevalent trend of HIV infections among pregnant women in Nanning City from 2009 to 2011 showed a stable trend, and sex transmission was the primary transmission of AIDS. The intervention for PMTCT is effective and feasible.But the prevention and control of AIDS is still a severe challenge. It is necessary to adopt effective preventive measures to control the prevalence.]]> 2018/1/24 8:18:04 JIANG Wu, HE Lin, MAO Liang-qin,et al. 648true ［Abstract］　Objective　To explore the short time effect of external high-frequency thermotherapy on pulmonary function recovery in patients undergoing massive whole-lung lavage(MWLL).Methods　Forty-eight male coal workers′ pneumoconiosis patients receiving MWLL were randomly divided into treatment group and control group. The treatment group was treated by domestic HG-2000 high-frequency thermotherapy instrument(controlled temperature：39～43 ℃；therapeutic time:40 min/time;once every other day; for 3 times),and the control group did not receive thermotherapy. The pulmonary function changes between two groups were compared before and after whole-lung lavage about TLC, FVC, MVV, DLCO, FEV 1.0%.Results　The difference of pulmonary function changes between two groups before and after lavage were no significant(P>0.05).Conclusion　External high-frequency thermotherapy has no evident effect on the recovery of postoperative pulmonary function in patients undergoing whole-lung lavage, and its effect on the patients undergoing whole-lung lavage or pneumoconiosis patients remains to be observed.]]> 2018/1/24 8:18:04 GUO Xian-yong,WANG Yue-gang,LI Guo-feng,et al. 647true ［Abstract］　Objective　To investigate the practical family rehabilitation model suitable for rural children with cerebral palsy.Methods　Based on the theoretical framework of International Classification of Functioning, Disability and Health(ICF), parents were guided to conduct one-year family rehabilitation for 50 rural children with cerebral palsy at home through concentrated training for parents, guidance by professional and technical personnel entering their homes as well as telephone interview.Results　The sports ability self-care ability of daily living and social adaptability of the 50 children with cerebral palsy were improved with a total rehabilitation efficiency rate of 88%.Conclusion　The ICF-based family rehabilitation model for rural children with cerebral palsy allows parents to do their daily work with children rehabilitation training, parents′ compliance is high and the rehabilitation of children can get long-term adherence with good results, thus it is worthy of promotion.]]> 2018/1/24 8:18:04 ZHANG Ming-wu, HUANG Yan-zhi, MENG Ai-zhen,et al. 646true ［Abstract］　Objective　To study the correlation between prehepatic fat thickness and insulin resistance indexes in infertility patients with polycystic ovarian syndrome(PCOS), and to provide theoretic basis for the clinical diagnosis and treatment of infertility patients with PCOS.Methods　The prehepatic fat thickness were detected by ultrasonography, and the height, weight, waist circumstance, the circle of hip line in 91 infertility patients with PCOS were measured, and sex hormones, insulin and thyroid hormones were detected, while the blood glucose, lipids, liver and renal function indexes were detected also. The relation among prehepatic fat thickness and the above indexes were analyzed.Results　There was a positive correlation among the prehepatic fat thickness and weight, body mass index(BMI), waist circumference, hip circumference（P<0.05）, while no significant relation was found between prehepatic fat thickness and height（P>0.05）. There was a positive correlation between the prehepatic fat thickness and fasting insulin, serum sugar, uric acid, insulin resistance index（P<0.05）. There was a negative correlation among the prehepatic fat thickness and high-density lipoprotein（P<0.05）, while no significant relation was found among prehepatic fat thickness and sex hormones, thyroid hormones, total cholesterol, triglyceride, low density lipoprotein（P>0.05）.Conclusion　There is a certain correlation between prehepatic fat thickness and insulin resistance indexes in infertility patients with PCOS, a complete diagnostic evaluation should be conducted in such patients before clinical diagnosis and treatment so as to improve their pregnancy rate and pregnant outcomes.]]> 2018/1/24 8:18:34 WAN Li-kai, HE Bing, TAN Wei-hong, et al. 645true ［Abstract］　Objective　To compare the effect between penalization and occlusion in the perceptual learning for treatment of school-age children with anisometropic amblyopia.Methods　In the perceptual learning for treatment of school-age children with anisometropic amblyopia,85 eyes of 43 patients received the penalization(penalization group) and 70 eyes of 35 patients received the occlusion(occlusion group).After 1 year researching was analyzed.Results　The effects of the penalization group were better than the occlusion′s,the effects in both slight and moderate amblyopia of the penalization group also better than occlusion′s,their difference had the statistical significance(P<0.05),but in the severe amblyopia,the difference of the effect between two groups had no statistical significance(P>0.05).Conclusion　In the perceptual learning for treatment of school-age children with anisometropic amblyopia,using the penalization may have higher total effect than using the occlusion.]]> 2018/1/24 8:18:34 KONG Min,LIN Quan,ZHAO Wu-xiao,et al. 644true ［Abstract］　Objective　To compare the effect between Totally video-assisted thoracic surgical technique(VATS) and conventional thoracotomy(CT) on beating heart in repairing atrial septal defect(ASD).Methods　Thirty patients with atrial septal defect were divided into 2 groups averagely(VATS group and CT group).In the VATS group, the atrial septal defects were repaired with video-assisted thoracic surgical technique and in CT group with conventional thoracotomy. The bypass time, the operative time and the postoperative mechanical ventilation time, chest drainage volume, days of postoperative hospital stay and the effect of surgical procedures were compared beteen two groups.Results　Between two groups, there were no differences in the bypass time,the operative time,and the postoperative mechanical ventilation time,and the effect of surgical procedures(P>0.05). However, there were differences in chest drainage volume, days of postoperative hospital stay, postoperative incision pain(P<0.05).The VATS group had a significantly less chest drainage volume, significantly shorter days of postoperative hospital stay.Conclusion　VATS has the advantages of shorter chest incision length,shorter time of recovery,less incision pain and so on.The ASD repair under VATS on beating heart was feasible.]]> 2018/1/24 8:18:34 ZHONG Qi-qing,DU Zheng-long,LING Yi 643true ［Abstract］　Objective　To probe into specific cellular immunity to EBV-LMP in advanced nasopharyngeal carcinoma patients.Methods　Peripheral blood mononuclear cell drawn from nasopharyngeal carcinoma patients were cultured in vitro. EBV-LMP₂ was added to prepare sensitized lymphocytes cells, which was injected into patients. Change in specific cellular immunity level was observed.Results　With sensitized lymphocytes cell injected specific cellular immunity level increased in nasopharyngeal carcinoma patients.Conclusion　Specific cellular immunity cure can be effective adjuvant therapy to the nasopharyngeal carcinoma patients.]]> 2018/1/24 8:18:34 ZHOU Wei-ya, ZENG Yi, ZHOU Ling 642true ［Abstract］　Objective　To explore the advantages and disadvantages of self-ligating bracket appliance in the treatment of malocclusion patients.Methods　The treatment information of 10 malocclusion patients had been treated voluntarily with self-ligating bracket appliance in department of orthodontics,the People′s Hospital of Guangxi Zhuang Autonomous Region from 2010 to 2013 were collected and their treatment course,method and effect were analyzed and summarised.Ten cases included 4 extracted cases,4 unextracted cases,one treated by moving molar distally and one having periodontopathy.Results　The longest treatment course was 26 months,the shortest 9 months,average (17±6.04)months. Dentitions were level and orderly.Overjet and overbite were normal range.Occlusion were compact.Conclusion　Self-ligating bracket appliance has special structure and could reduce friction between arch and bracket,so reduce drawing force.Teeth could be leveled fast. and orderly course of dentition was short.Pain during treatment was light.So self-ligating appliance was effective.]]> 2018/1/24 8:18:34 LU Shi-ying,TAN Gui-ping,HUANG Min-fang 641true ［Abstract］　Objective　To explore the related factors of tooth loss and dentine restoration in patients with type 2 diabetes.Methods　One hundred and twenty -six individuals were divided into experimental group and control group according to whether with diabetes mellitus. The tooth loss and restoring between two groups wewe compared to find the relationship between tooth loss and related factors of periodontal disease in patients with type 2 diabetes.Results　Experimental group showed a significantly higher in the number of tooth loss, periodontal problems and severe defect of dentition(P<0.05). Logistic multiple regression analysis came to the result that patients with type 2 diabetes showed a significantly higher in the number of tooth loss and a significantly increase in number of tooth with periodontal problems, severe defect of dentition and lack of regular oral health examination(P all <0.05).Conclusion　These study results revealed that the tooth loss in patients with diabetes has a close correlation with the number of tooth with periodontal disease, severe defect of dentition , regular oral health examination.]]> 2018/1/24 8:18:34 ZHOU Yan,PENG Li-hui，LIU Hong-yan, et al. 640true ［Abstract］　Objective　To investigate the effects of low-molecular-weight heparin calcium（LMWH） in patients with primary severe nephritic syndrome(PSNS).Methods　Forty patients with PSNS were divided randomly into treated groups(n=21) which was treated with LMWH of 5 000 U·d^-1 injection under skin and control group(n=19) which was treated with Dipyridamole 50 mg three times every day for 4 weeks. Two groups were treated with the same base of steroid and Fenofibrate. Plasma FIB, 24 h urinary protein, Scr, ALB, TCHO, TG, PLT, PT and APTT were observed.Results　A significant decrease of plasma FIB, serum TCHO and TG was observed in the treated group compared with those in the control group after 4 weeks of treatment(P<0.05). A significant decrease of 24 h urinary protein and increase of serum ALB was observed in the treated group compared with those in the control group after 4 weeks of treatment(P<0.05). Only 2 of the 21 patients in treated group complained of ecchymosis under skin.Conclusion　The treatment with LMWH for PSNS can significantly decrease plasma FIB and protect against high thrombotic risk of PSNS, LMWH for PSNS can significantly decrease 24 h urinary protein, serum TCHO, TG and increase serum ALB, and it is helpful to relieve PSNS.]]> 2018/1/29 9:14:33 HUANG Wen-tan,GONG Zhi-feng,PENG Xiao-mei,et al. 639true ［Abstract］　Objective　To investigate the clinical features, diagnosis, treatment and prognosis of pulmonary cryptococcosis.Methods　The medical data of 23 cases of proven pulmonary cryptococcosis were studied retrospectively.Results　Twenty-three cases were anti-HIV negative, 21 cases(91.3%) were primary pulmonary cryptococcosis and 2 cases(8.7%) were secondary ones. Cough(13 cases, 56.5%) and chest pain(9 cases, 39.1%) were the most common symptoms. Subpleural nodule/mass(18 cases, 78.3%) in the lower lobes(12 cases, 52.2%) were common radiologic findings. The definite diagnostic techniques included surgical lung biopsy(12 cases, 52.2%) and CT guided percutaneous lung biopsy (10 cases, 43.5%). Ten patients received surgical resection lesions and 10 internal patients were treated with fluconazole and 18 cases were cured.Conclusion　Primary pulmonary cryptococcosis also occurs frequently. Radiologic findings can provide the clue for diagnosing pulmonary cryptococcosis. CT guided percutaneous lung biopsy is the most costless and efficient diagnostic technique. Most pulmonary cryptococcosis patients respond well to fluconazole.]]> 2018/1/29 9:14:33 CHEN Gui-rong, QIN Zhi-qiang, WEI Hai-ming, et al. 638true ［Abstract］　Objective　To discussed the feasibility，validity and effect of lumbar unilateral fixed intervertebral fusion via Mast Quadrant minimally invasive system.Methods　From May 2010 to May 2012,in our hospital,there were 22 patients undergoing surgery of lumbar unilateral fixed intervertebral fusion via Mast Quadrant minimally invasive system(the minimally invasive and unilateral fixed group),and 25 patients undergoing open surgery of lumbar bilateral fixed intervertebral fusion(the open and bilateral fixed group).Results　In these respects such as length of incision, time of operation, intraoperative blood loss,postoperative hospitalization duration and postoperative wound pain duration, the minimally invasive and unilateral fixed group was superior to the open and bilateral fixed group(all P<0.01). One year after operation,the Japanese orthopeadic association(JOA) scores was higher and visual analog scale(VAS) scores was lower in the minimally invasion and unilateral fixed group than the open and bilateral fixed group(all P<0.01).While in the height of intervertebral space and the intervertebral fusion rate there was no statistically significant difference between the two groups（P>0.05）.Conclusion　The surgery of lumbar unilateral fixed intervertebral fusion via Mast Quadrant minimally invasive system and the surgery of lumbar bilateral fixed intervertebral fusion have same clinical effects in recovery of intervertebral space height,increasing of intervertebral fusion rate and reconstruction of lumbar spinal stability.However, the minimally invasive and unilateral fixed intervertebral fusion has advantages of less operative wound, shorter operative and hospitalizing time, more rapid recovery and fewer complications.]]> 2018/1/29 9:14:33 LIANG Bin, LI Rong-zhu, WEI Jian-xun, et al. 637true ［Abstract］　Objective　To observe the heart rate variability(HRV) change in senile non-phacoemulsification, to explore the influence of cataract surgery on perioperative cardiac autonomic nervous system(ANS) function, and to provide a theoretical basis for evaluation of surgical risk.Methods　Using prospective method, 60 senile cataract patients(60 eyes) aged over 60 without cardiovascular disease histories were randomly selected; under local anaesthesia, all patients underwent non-phacoemulsification extracapsular cataract extraction plus intraocular lens implantation surgery. 24 h dynamic electrocardiogram(DCG) was recorded for measurement of HRV frequency domain indexes at 17 h one day before surgery and at 17 h the post-operative day. Observed the senile patients, and the frequency domain index(LF, HF and LF/HF) changes from 0 h to 5 h before surgery, during, and 12 h to 17 h after surgery were recorded. The detecting data and calculation material were statistically analyzed in each group, and the changes′ characteristics and reasons were compared.Results　In the perioperative period, all patients were found that frequency domain index during and after surgery were significantly lower than those before surgery(P<0.05 or P<0.01). The HRV frequency domain before surgery was significantly lower than that after surgery(P<0.05 or P<0.01). The results showed that under local anesthesia, HRV during and after surgery were significantly lower than that before surgery in patients who undergone non-phacoemulsification extracapsular cataract extraction plus intraocular lens implantation surgery, which declined more significantly during surgery.Conclusion　The stress simulations from local anesthesia and cataract surgery can evidently influence ANS function in patients with senile cataract, which prompt that both during and after surgery may be the periods easy to trigger cardiovascular events, and corresponding prevention measures should be taken to reduce the surgical risk.]]> 2018/1/29 9:14:33 SUN Zhong-bo，LIANG Jian-yi，ZENG Si-ming，et al. 636true ［Abstract］　Objective　To investigate the clinical value of Epstein-Barr virus (EBV) Rta/IgG in the diagnosis of nasopharyngeal carcinoma(NPC).Methods　Sera from 612 clinical samples were measured for the Rta-IgG by an indirect enzyme-linked immunosorbent assay. In the present study, we also evaluated the performance of the Rta-IgG ELISA kits.Results　The mean value of Rta-IgG concentration in 267 NPC patients was (77.64±3.205)U/ml with a sensitivity of 94.7%(214/226) and specificity of 86.3%(333/386) and accuracy of 89.4%(547/612). The mean value of Rta-IgG concentration in 255 healthy volunteers，20 hepatocellular carcinoma patients，30 hepatitis patients，20 patients with autoimmune disease and 20 patients with nasopharyngeal polyp were (8.31±0.745)U/ml, (7.14±1.111)U/ml, (7.40±0.581)U/ml, (13.97±6.943)U/ml and (12.58±6.197)U/ml, respectively. There were significant difference between NPC group and other groups.Conclusion　Our results suggest that the EBV Rta/IgG ELISA kit is suitable for the clinical diagnosis of NPC. It also provides a basis for further study on the relationship between the therapeutic effect and serum EBV Rta-IgG content in NPC patients.]]> 2018/1/29 9:14:33 QIN Gui-fang，LI You -qiong，LU Qiu-wei，et al. 635true ［Abstract］　Objective　To explore the therapeutic effect of combination use of oxidation-atomization inhalation and intravenous drop of ambroxol and their separate use in the treatment of children bronchial pneumonia.Methods　From September 2011 to March 2013 101 children with bronchial pneumonia, according to the admission time, were randomly divided into A, B, C groups.On the basis of comprehensive treatment, group A was treated with oxidation-atomization inhalation combined with intravenous drop of ambroxol, group B with oxidation-atomization inhalation of ambroxol, group C with intravenous drop of ambroxol. The comparison between 3 groups was performed of the total efficiency, the change of chest X-ray, days of hospitalization and symptom improvement time.Results　(1)There was no statistically difference in total effective rate between 3 groups(P>0.05);(2)There was no statistically difference in clinical indexes(symptom improvement,day of hospitalization) between 3 groups(P>0.05);(3)After 7 days of treatment there was no statistically difference in change of chest X-ray between 3 groups(P>0.05);(4)No statistical significance among the 3 groups in changes WBC and CRP(P>0.05). But the number of cases of hs-CRP returned to normal in A group were more than B or C group(P<0.05).Conclusion　The efficacy and the clinical index changes after treatment, WBC, chest X-ray and CRP of ambroxol oxidation-atomization inhalation combined with intravenous infusion of ambroxol in the treatment of children bronchial pneumonia is not superior to treatment with intravenous infusion or oxygen-driven inhalation of ambroxol, only there is advantage to restore hs-CRP, consider the combination of two routes of drug use should waste pharmaceutical sesource, increase the burden of patients, and waste of the nursing human resource.]]> 2018/1/29 9:14:33 DUAN Jie-hua, FAN Xiao-yang, QIN Rui, et al. 634true ［Abstract］　Objective　To investigate the effects of neonatal ICU noise on newborn hearing and intelligence.Methods　Fifty-five newborns in department of neonatology treated by the ventilator were randomly divided into observation group(n=28) and the control group(n=27).The observation group used earmuffs, while the control group did not use earmuffs. The brainstem auditory evoked potential, cranial utrasound, skull CT and regular medical examinations including mental development tests were carried out in the children of two groups after the treatment of ventilator.Results　The total hearing loss and the proportion of mild hearing loss was significantly lower in the observation group than the control group(P<0.05). There was no significant difference between the two groups in moderate and severe hearing loss(P>0.05). At 6 months after birth, the differences in total hearing loss, mild hearing loss moderate and severe hearing loss were not statistically significant between the two groups(P>0.05). The NBNA, DQ value of 6 and 12 months were higher in the observation group（P<0.05 or P<0.01） while the prognosis rate lower than the control group.Conclusion　Neonatal ICU noise can cause the mild hearing damage and have influence on intelligence in newborns, while wearing earmuffs can reduce the adverse impact on newborn hearing and intelligence.]]> 2018/1/29 9:14:33 LIAO Ji-wen, GU Yu-fang, RUAN Bang-wen,et al. 633true ［Abstract］　Objective　To investigate the relationship between inflammatory reaction and formation of carotid plaque in hypertension patients, and to find out optimum way to prevent and treat these diseases.Methods　Two hundred and seventy-six hospitalized hypertension patients with or without carotid plaque treated in our hospital from June 2009 to March 2013 were enrolled in this study, including 192 patients with carotid plaque and 84 patients without carotid plaque. Hypersensitive C-reactive protein(hs-CRP) in all patients were detected routinely. All patients were detected carotid plaque by Colour Doppler Ultrasound.and try to know about the relationship between hs-CRP and formation of carotid plaque in hypertension patients.Results　In hypertension patients, those with carotid plaque have higher hs-CRP than that in those without carotid plaque（P<0.01）.Conclusion　Inflammatory reaction has close relative with the formation of carotid plaque in hypertension patients.]]> 2018/1/29 9:14:33 PAN Qing-deng, LU Zhi-dong, HUANG Yong-kang, et al. 632true ［Abstract］　Objective　To explore the efficacy and safety of stent-assisted coiling embolization for the treatment of intracranial wide-necked aneurysms.Methods　Stent-assisted coiling technique was used for embolization treatment in 25 patients with intracranial wide-necked aneurysms.Results　The total occlusion was obtained in 19 cases and suntotal occlusion in 6 cases.There were no aneurysm rupture,parent artery spasm and thrombosis complications occurred during the operation.During clinical follow up,all patients′s GOS score were above 3 points and no death was found.DSA or CTA showed all patients recovered well with the patency of the parent arteries，no displacement and stenosis of stent were found,and no aneurysm recurred.Conclusion　For the treatment of intracranial wide-necked aneurysm，stent-assisted coiling embolization technique is a safe and effective therapy.]]> 2018/1/29 9:14:33 CHEN Yuan,XIAO Ji-dong,CHEN Zhi,et al. 631true ［Abstract］　Objective　To observe the effects of different doses of atorvastatin on phosphorylation of nuclear factor-kappa B(NF-κB) P65(ser536) in peripheral blood mononuclear cells, on the levels of serum inflammatory factors，including high sensitivity C reactive protein(hs-CRP),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)，interleukin-1β(IL-1β), and on urinary albumin excretion rate(UAER) in the elderly patients with early diabetic nephropathy.Methods　The patients(n=72) of type 2 diabetes with early nephropathy were randomly divided into two groups,The patients were given atorvastatin 10 mg/d in DN1 group and atorvastatin 20 mg/d in DN2 group for sixteen weeks. Levels of NF- κB, serum inflammation factors,UAER，Total cholesterol(TC),triglyceride(TG),low density lipoprotein(LDL-C) and hight density lipoprotein(HDL-C) were measured before treatment and after treatment for sixteen weeks.Results　After sixteen weeks of treatment, the levels of NF-kB, serum inflammation factors, TC and LDL were reduced(P<0.05 or P<0.01) in two group. DN2 group decreased more significantly. UAER were reduced(P<0.01)in DN2 group. There was no significant changes in DN1 group(P>0.05).Conclusion　Atorvastatin can reduce activity of NF-κB, levels of hs-CRP,TNF-α,IL-6,IL-1β,TC, and LDL in the olderly patients with type 2 diabetic pristine nephropathy. Atorvastatin may benefit patients with diabetes mellitus by ameliorating inflammatory stats, as well as reducing urinary albumin,in a dose-dependent manner.]]> 2017/12/25 16:02:34 LI Jian-ying, GUO Jin, TAN Wen-fang, et al. 630true ［Abstract］　Objective　To investigate the predictive value of ACS by brachial artery FMD and sCD40L.Methods　Thirty-nine healthy volunteers（control group）, 39 patients with stable angina pectoris(SA group) and 79 patients with acute coronary syndrome(ACS)，including 49 patients with unstable angina pectoris(UA group) and 30 patients with acute myocardial infarction(AMI group), were radomly selected. The patients with ACS were detected the brachial artery FMD by ultrasonic testing and the serum sCD40L by ELISA.Relation of brachial artery FMD and serum sCD40L of ACS patients were analyzed by using Pearson correlation analysis.Results　Compared with control group and SA goup, the brachial artery FMD decreased in UA group and AMI group （9.52±2.54）%(control group) vs （5.35±1.24）%(SA group) vs （3.32±1.41）% (UA group) vs （3.26±1.32）%(AMI group), （P<0.05）. Compared with control group and SA goup, the serum sCD40L increased in UA group and AMI group （1.21±0.31）ng/ml(control group) vs （4.12±0.98）ng/ml(SA group) vs （12.86±2.67）ng/ml (UA group) vs (13.95±2.83)ng/ml(AMI group), (P<0.05）. Pearson correlation analysis indicted that the brachial artery FMD was negatively associated with the level of serum sCD40L in ACS patients (r=-0.708，P=0.000).Conclusion　The downregulation of brachial artery FMD by ultrasound findings and upregulation of serum sCD40L have predictive value for the occurrence of ACS.]]> 2017/12/25 16:02:34 ZHAO Juan, ZHANG Xiao-yong, DENG Cai-mei, et al. 629true ［Abstract］　Objective　To evaluate the effects of two groups of cryoprotectants on cleavage stage embryos.Methods　A total of 102 discarded embryos on day 3 after fertilizaion from IVF/ICSI-ET cycles were randomly allocated to group of ethylene glycol+propanediol(EG+PROH) （52 embryos）and group of ethylene glycol + dimethyl sulphoxide(EG+DMSO)（50 embryos）. After warming, the survivals efficient of embryos between the two groups were compared.Results　After warming, the two groups had a similar survival rate, but the intact survival rate and blastomere survival rate of cleavage stage embryos in the EG+PROH group was significantly higher than those in the EG+DMSO group(P<0.05）.Conclusion　For the vitrification of cleavage stage embryos, using EG+PROH as a cryoprotectant can achieve good results.]]> 2017/12/25 16:02:34 WANG Shi-kai, HUANG Li, HE Bing, et al. 628true ［Abstract］　Objective　To investigate the value of the ribs-reserved intercostal space approach in the middle and lower thoracic vertebrae surgery.Methods　The operation via intercostal space without breaking the ribs were performed on 40 patients with the middle and lower thoracic vertebrae disease（group A）, and compared with the control group which contained 40 cases accepting the traditional lateral ribs-broken methods（group B）. The difference between the two groups were compared in the operative incision length, vertical diameter of the surgical field, thoracotomy time, thoracic operation time, time of closing thoracic incision, peri-operative bleeding volume, placement time of drainage tube, postoperative drainage, postoperative pain visual analogue scale(VAS), respiratory function before operation and at one week after operation as well as postoperative hospitalization duration.Results　The results of group A were superior to the group B in incision length, thoracotomy time, time of closing thoracic incision, blood loss, placement time of drainage tube, postoperative drainage and postoperative hospitalization duration(P<0.05); VAS score of one month after the operation of group A was better than that of group B, the difference was statistically significant(P<0.05). However, there was no significant difference of 3 months after operation(P>0.05); preoperative lung function scores between the two groups showed no difference(P>0.05 ), but the lung function at one week after operation of group A was better than that of group B(P<0.05); The outcomes between the two groups showed no difference in the fields of surgical field longitudinal diameter and operating time(P> 0.05).Conclusion　The application of the ribs- reserved intercostal space approach for the treatment of thoracic vertebrae disease has the advantage of being able to save time, exposing operative field fully, curbing the bleeding, making simple incision, causing less postoperative pain, getting faster recovery, improving the quality of life, and is worth to be generalized.]]> 2017/12/25 16:02:34 YAN Dong-xue, MA Guang-bin, HUANG Yong-ji, et al. 627true ［Abstract］　Objective　To investigate the 64-slice volume CT findings and differential diagnosis of the pulmonary atypical adenomatous hyperplasia(AAH).Methods　The data of 64-slice volume CT image of 12 cases of pathologically confirmed pulmonary AAH was made retrospectively. The CT data were compared with those of the 76 cases of localized ground-glass opacity(GGO) in terms of the lesion location,size,shape,edge signs,internal structure and relationship to adjacent structures.Results　Pure ground-glass density nodules were commonly seen in AAH group,pure ground-glass density and mixed-density nodules were seen in benign group,and mixed-density nodules account for the majority in malignant group.There were statistical differences between AAH group and benign or mglignant GGO group in the aspects of lobulation sign,spicular sign,pleural indentation sign and vascular convergence sign（P<0.05）. However,there showed no significant differences in the signs of vacuole sing and air branchogram between the AAH group and benign GGO group or malignant GGO group（P>0.05）.Conclusion　Pure ground-glass density nodules are the main constituent seen in AAH group.The nodules′ diameter were commonly less than 10mm. And no lobulation sign,spicular sign,pleural indentation sign or vascular convergence sign can be seen in the AAH group.It can do help in differential diagnosis analyzing the CT value of the solid component.However,only the histopathology result is the real and the last diagnosis.]]> 2017/12/25 16:02:34 SUN Zhen-chao, LI Jia-de 626true ［Abstract］　Objective　To explore the effects of bipolar electrocoagulation hemostasis versus suture hemostasis of surface of the wound in laparoscopic cystectomy for chocolate cyst of ovary on ovarian sexual hormones secretion.Methods　Eighty patients with unilateral ovarian cyst who underwent laparoscopic cystectomy were randomly divided into two groups：bipolar electrocoagulation group and suture group,with 40 cases in each group.Patients in bipolar electrocoagulation group received bipolar electrocoagulation for hemostasis，and patients in suture group received suture for hemostasis.The serum levels of follicle stimulating hormone(FSH), luteinizing hormone(LH), estradiol(E₂) were detected respectively before the operations and at 1st month,3rd month and 6th month after operation.Results　（1）In bipolar electrocoagulation group, compared with the levels before operation,the E₂ level had obviously reduced, FSH and LH levels had obviously risen at the 1st month after operation, the differences had statistically significance(P<0.05); the E₂ level had slightly risen, FSH and LH levels had slightly reduced at the 3rd month after operation, the differences had statistically significance(P>0.05); the E₂, FSH and LH level had returned to the levels before operations at the 6th month after operation（P>0.05）. (2)In suture group, compared with the levels before operation, E₂ level had reduced, FSH and LH levels had risen at the 1st month after operation, the differences had statistically significance(P<0.05); the E₂ level had slightly risen, FSH and LH levels had slightly reduced at the 3rd month after operation, the data had no statistically significance(P>0.05); the E₂, FSH and LH level had returned to the levels before operations at the 6th month after operation（P>0.05）; Compared with the suture group, the bipolar electrocoagulation group had more influence on ovarian sexual hormones secretion（P<0.05）.Conclusion　Both of the two hemostatic methods in laparoscopic cystectomy for ovarian endometriotic cyst have effects on sexual hormones secretion,especially electrocoagulation hemostasis has more.In most of patients the ovarian sexual hormones secretion can be recovered to normal level basically in 6 months after operation.]]> 2017/12/25 16:02:34 LIU Yuan-yuan, ZHAO Ren-feng 625true ［Abstract］　Objective　To explore the effect of application of delivery ball combined with touching during delivery among primipara.Methods　Four hundred primipara women who would deliver had head position without pregnancy complications, and had the uterus cervix at 3 cm wide were recruited in the observation. The women were allocated to the observation(n=200) or the control group(n=200) according to the sequence of admission to the hospital. The primiparas in the observation group received delivery ball combined with touching and those in the control group only received delivery ball. The labor time, the degree of pain, the mode of delivery, the amount of bleeding within 2 hours after delivery and neonatal apgar scores were observed in the two groups.Results　The time of the first stage and the total stage of labor, and the degree of pain in the observation group were statistically different in comparison to the control group(P<0.05). However, there was no statistical difference in delivery mode, the time of the second and the third stage of labor, the amount of bleeding within 2 hours after delivery and neonatal apgar scores between the two groups(P>0.05).Conclusion　Application of delivery ball combined with touching can relieve pain, promote the progress of labor, and shorten the time of stage of labor among primipara women, and it is worthy of clinical application.]]> 2017/12/25 16:02:34 DOU Zhi-ying, LIANG Yan-fang, HUANG Mei, et al. 624true ［Abstract］　Objective　To provide a reference for proper use of ephedrine by observing impacts of nasal drop of 1% ephedrine hydrochloride on blood pressure of patients treated with hyperbaric oxygen(HPO).Methods　Sixty HBO patients were randomly divided into two groups(30 cases for each), i.e. observed group and contrast group. Patients in observed group, before being sent into large medical hyperbaric oxygen tank, were put 1% ephedrine hydrochloride nasal drops three drops into bilateral nasal cavities while those in contrast group won′t. Their blood pressure were measured at the right upper limb in different period of time, i.e. before HBO, at 20 min of pressurizing, at 25 min and 50 min of oxygen inhalation under stable pressure, before finishing of decompressing, at 30 min after HBO. Their blood pressure change were observed and statistical analysis was carried out.Results　For systolic pressure and diastolic pressure, there was no significant difference between observed group and contrast group(P>0.05). The blood pressure of both groups increased first and then decreased during HBO, it had statistical significance comparing with prior treatment(P<0.05). But there was no interaction between groups and time-points, that showed, impacts on blood pressure treating with or without nasal drop were the same.Conclusion　For those patients who is in normal blood pressure,taking nasal drop of 1% ephedrine hydrochloride three drops into bilateral nasal cavities before HBO does not affect blood pressure.]]> 2017/12/25 16:02:34 KONG Lei, FU Gui-fen, XIE Jin-ping, et al. 623true ［Abstract］　Objective　To analyse and discuss the clinical curative effect of endovascular embolization of intracranial aneurysm for rupturing intracranial aneurysm in elderly patients(>70 years old).Methods　Forty-five patients(>70 years old) were selected, subarachnoid hemorrhage by CT examination and aneurysm by cerebral angiography.According to Hunt-Hess classification: there were 32 cases in Ⅱ～Ⅲ class, and 13 cases above Ⅳ class. Forty-eight aneurysms we found through digital subtraction angiography. According to the different structure and position of aneurysms, which were sent with or without coil and bollooon assisted for endovascular embolization. After operation, they were treated by “3H” and nimodipine for preventing cerebral vasospasm. At the same time, the basic diseases were treated.Results　Successful embolization was performed in 45 aneurysms, the embolization success rate was 91.67%. The scores of operation showed, 37 cases were excellent; 5 cases were mild disability; 1 case was disability and 2 cases were death.Conclusion　Endovascular embolization can effectively treat the rupturing intracranial aneurysms in patients older than 70 years, and prevent aneurysms ruptured bleeding again.]]> 2018/1/2 8:38:52 XIAO Quan，ZHANG Guo-zhi，CHEN Hai-jun，et al. 622true ［Abstract］　Objective　To understand the survival situation and its influencing factors in patients with AIDS, to explore the strategy prolonging the survival time of patients with AIDS.Methods　Clinical data of 305 of AIDS patients from January 2010 to December 2012 were retrospectively analyzed, and the COX proportional hazard model was used to analyze the risk factors affecting survival rate.Results　At the end point of the study,of 305 patients, 204 patients (66.9%) died, 101 patients (33.1%) survived.At 1～3 months the cumulative survival rates were 45%, 25%, 11% respectively. The median survival time was 27.4 d. Multivariate COX proportional hazard model analysis showed that the risk of death in patients were infected via sexual transmission and other ways was lower than that in patients were infected via intravenous route(HR=0.878, HR=0.373). The risk of death in patients with higher baseline of CD4⁺ T lymphocyte count was lower than that in patients with lower baseline of CD4⁺ T lymphocyte count(HR=0.157, HR=0.423). The patients received antiviral treatment at admission had lower risk of death(HR=0.378)；The patients with fever(HR=3.033), and associated encephalopathy(HR=1.678) at admission had higher risk of death.Conclusion　AIDS hospitalization survival time is relatively short, the route of infection, baseline of CD4⁺ T lymphocyte count, whether fever at admission, whether merger AIDS-associated encephalopathy, and whether use the antiviral treatment is the main factors influencing the survival time of hospitalization.]]> 2018/1/2 8:38:52 ZHANG Yi-ling, CAO Yong-hong, ZHANG Yong, et al. 621true ［Abstract］　Objective　To evaluate the application of sequential noninvasive following invasive mechanical ventilation in chronic obstructive pulmonary disease （COPD） patients with severe respiratory failure by investigating the appearance of respiratory pump failure improved window（RPFIW）.Methods　COPD patients with severe respiratory failure receiving invasive mechanical ventilation（MV）were involved in the study. When the RPFIW appeared by the antibiotic and comprehensive therapy，106 cases were randomized into invasive-noninvasive sequential mechanical ventilation group and control group. The early extubation was conducted and followed by noninvasive mechanical ventilation via facial mask immediately in the sequential mechanical ventilation group. Conventional invasive mechanical ventilation was used as the weaning technique in the control group. The incidence of ventilator-associated pneumonia (VAP)，incidence of reintubation and mortality，the duration of invasive mechanical ventilation and days in ICU were analyzed and compared.Results　There were 52 cases in the sequential mechanical ventilation group，54 cases in the control group. In the sequential mechanical ventilation group，the duration of invasive mechanical ventilation was（3.9±1.5）d，while the duration was（15.5±6.1）d in the control group （P<0.05）. The durations of ICU stay was（4.9±1.8）d in the sequential mechanical ventilation group， （20.3±5.5）d in the control group（P<0.05）. The incidence of VAP was 15.38%（8/52） in the sequential mechanical ventilation group，35.19%（19/54） in the control group respectively （P<0.05）. Hospital mortality was 11.54%（6/52） in the sequential mechanical ventilation group，and 27.78%（15/54） in the control group （P<0.05）. The incidence of reintubation was 19.23%（10/52） in the sequential mechanical ventilation group，16.67%（9/54） in the control group （P>0.05）.Conclusion　In those COPD patients requiring invasive mechanical ventilation with severe respiratory failure due to respiratory pump failure，sequential noninvasive following invasive mechanical ventilation at the appearance of respiratory pump failure improved window might significantly reduce duration of invasive mechanical ventilation，and days in ICU stay，and decrease the occurrence of VAP and hospital mortality.]]> 2018/1/2 8:38:52 LONG Sheng-ze, QIN Zhi-qiang, HUANG Guo-lan, et al. 620true ［Abstract］　Objective　To analyse the postoperation complications of minimally invasive transforaminal lumbar interbody fusion(MIS-TLIF) and traditional open posterior lumbar intervertebral fusion(OPEN-PLIF) retrospectively, and to investigate its characteristics and prevention.Methods　An analysis was performed on 265 patients who underwent MIS-TLIF(Group MIS-TLIF，125 cases) or PLIF(Group PLIF，140 cases). Postoperative outcomes and complications were recorded and statistically analyzed.Results　In the length of the incision, intraoperative blood loss, postoperative flow and postoperative hospital stay, etc, the MIS-TLIF group was superior to the OPEN-PLIF group(P<0.05). In group MIS-TLIF, postoperation complications included nerve root irritation in 1 cases, wound infection in 1 case, cerebrospinal fluid leakage in 1 case, urinary system diseases in 1 case, digestive system disease in 1 case, cardiopulmonary system diseases in 2 cases, Internal fixation complication in 1 case; In group PLIF，postoperation complications included nerve root irritation in 8 cases, wound infection in 2 cases, cerebrospinal fluid leakage in 3 cases, urinary system diseases in 2 cases, digestive system disease in 2 cases, hematological disease in 3 cases, cardiopulmonary system diseases in 2 cases, Internal fixation complication in 2 case. There was significant difference in total complication rate between 2 groups(P<0.05).Conclusion　In the treatment of lumbar degenerative disease, MIS-TLIF is safe and effective, and it has the advantages such as less damage, lower complication rates and faster recovery.]]> 2018/1/2 8:38:52 LI Hua, LI Rong-zhu,WEI Jian-xun, et al. 619true ［Abstract］　Objective　To study the significance of preoperative interventional treatment of primary retroperitoneal neoplasm in reducing bleeding, improving complete resection rate and reducing surgical-associated mortality. Methods　Clinic data were analyzed on surgical cases of primary retroperitoneal neoplasm retrospectively from October 2004 to December 2013. Forty-eight cases were selected in this study, including 12 preoperative interventional treatment cases(experimental group) and 36 non-preoperative interventional treatment cases(control group) respectively. The clinical indexes included volume of bleeding, completed resection rate and surgical-associated mortality rate. Results　In the experimental group, 10 cases were achieved completed tumor resection and 2 cases palliative tumor resection. There was no death in this group. The volume of bleeding was (1976.67±273.34 )ml. In the control group, 18 cases were achieved completed resection, 18 cases palliative tumor resection. One case was died of large bleeding in the operation, and 2 cases died of MODS postoperatively. The volume of bleeding was (2597.22±801.24) ml. In the experimental group, the volume of bleeding was less(P<0.05) and the complete resection rate was higher than that in the control group(P<0.05). There was no significant differences between two groups in surgical-associated mortality rate(P>0.05).Conclusion　This results indicate that preoperative interventional treatment can reduce operative bleeding and improve complete resection rate for primary retroperitoneal neoplasm. However, preoperative interventional treatment does not seem to affect the surgical-associated mortality rate in these study.]]> 2018/1/2 8:38:52 WU Dong-bo, FENG Ze-rong, LIU Yang-hua, et al. 618true ［Abstract］　Objective　To investigate the clinical characteristics laboratory examination, early diagnosis, the basis of classification and treatment of the patients with neurosyphilis.Methods　The data of 38 patients with neurosyphilis from September 2004 to January 2014 in Fourth People′s Hospital Affiliated to Jiangsu University, Zhenjiang First People′s Hospital and the Fourth People′s Affiliated Hospital of Jiangsu University were analyzed retrospectively.Results　In this group, there were 38 patients, male 28 cases, female 10 cases, age 1～75 years old, mean 51.1 years, including 10 cases of interstitial neurosyphilis(8 cases of meningovascular neurosyphilis , 1 cases of spinal meningovascular neurosyphilis, 1 patients with meningeal neurosyphilis), 22 cases of parenchyma type(14 cases of paralytic dementia, 8 cases of tabes dorsalis), 2 case of optic nerve injury, 3 cases of asymptomatic neurosyphilis, 1 cases of congenital syphilis; serum, CSF RPR and TPPA in all patients were positive. In part of patients cerebrospinal fluid examination showed elevated cerebrospinal fluid protein content, the increased white blood cell count(mainly monocytes). Except 3 cases giving up treatment, 35 cases recovered after treatment, the serum antibody titre and CSF antibody titers were reduced to varying degrees, CSF white blood cell count, protein decreased to normal.Conclusion　Neurosyphilis is preventable and curable, cerebrospinal fluid test for syphilis is an important basis for the diagnosis of neurosyphilis, but it is necessary to make a comprehensive analysis based on clinical manifestations, other examination in order to obtain the final diagnosis, and correct and timely treatment can control the disease.]]> 2018/1/2 8:38:52 XIA Hai-ping， REN Nai-yong, Qian Jin-jun，et al. 617true ［Abstract］　Objective　To explore the therapeutic effect of Shenque paste acupoint sticking in the adjuvant treatment of children bronchial pneumonia.Methods　From February 2012 to August 2013, 82 babies betwceen 1 month and 12 months who had bronchial pneumonia, were randomly divided into observation group and control group, both groups were treated with routine comprehensive treatment, on the basis of a comprehensive treatment the observation group received Shenque paste acupoint as adjuvant therapy, the control group received only conventional treatment.Results　(1)The total efficiency in the observation group was better than that in control group(P<0.05);(2)The clinical indexes of two groups(symptoms, signs disappeared time and length of stay) had no significant difference(P>0.05);(3) 7 d after treatment, chest radiograph recovery in the observation group was significantly better than the control group(P<0.05);(4)In two groups, there were no obvious adverse reactions after treatment there were no significant difference in WBC, GRAN#, CRP, HsCRP, HGB, MCV between two groups(P>0.05).Conclusion　Shenque paste acupoint sticking therapy is helpful to improve the curative effect of children bronchial pneumonia and promote chest radiograph recovery, is a method of adjuvant treatment for children bronchial pneumonia, and has the advantages of exact curative effect, simple operation lower price, non-toxic side effect and so on, is worth the clinical promotion.]]> 2018/1/2 8:38:52 DUAN Jie-hua, FAN Xiao-yang, ZHANG Jun-hua, et al. 616true ［Abstract］　Objective　To investigate the clinical efficacy of acupoint embedding in treating insomnia.Methods　Sixty insomnia patients were randomly divided into two groups: the treatment group(n=30) was given acupoint embedding,the control group(n=30) was given conventional alprazolam.Results　In the treatment group,18 cases were cured,9 cases improved and 3 cases were not cured,and in control group, 13 cases were cured,7 cases improved and 10 cases were not cured. there was a significant difference between the two groups(P<0.05).Conclusion　Acupoint acupoint embedding has a good therapeutic effect on insomnia with low recurrence rate, worthy to be popularized.]]> 2018/1/2 8:38:52 YE Cui-he, HE Qing-jun, HE Yi-hui, et al. 615true ［Abstract］　Objective　To investigate the expression of vasoactive intestinal peptide receptor(VIPR) mRNA in gastric adenocarcinoma and AGS cells, and understand the effect of VIPR on gastric adenocarcinoma and possible mechanism.Methods　Gastric tissues of 24 patients with gastric adenocarcinoma and 24 healthy controls were collected, and the expression of VIPR mRNA was determined by real time PCR. The VIPR mRNA expression was also detected in gastric adenocarcinoma cell line ACG by the same way.Results　The expression rate of VIPR1 mRNA was 45.00%(9/24)in gastric adenocarcinoma, which was significantly lower than controls 83.33%(20/24)(P<0.05), but that of VIPR2 mRNA didn′t changed significantly(50.00% vs 66.67% )(P>0.05). The levels of VIPR1 mRNA in gastric adenocarcinoma was lower than that in controls(P<0.05), while there was no different in the levels of VIPR2 mRNA(P>0.05). All of the AGS cells were positive both in VIPR1 and VIPR2 mRNA expression.Conclusion　The lower level of VIPR mRNA expression may be an integral part in the pathogenesis of gastric adenocarcinoma, and VIPR mRNA detection may be contribute to the diagnosis of gastric carcinoma.]]> 2018/1/2 8:39:31 CHEN Hui, LIAO Yan-yan, MO Lin-fang, et al. 614true ［Abstract］　Objective　To compare the clinical results between the mini-incision transforaminal lumbar interbody fusion(mini-incision TLIF) and conventional open approach in treatment of lumbar spondylolisthesis.Methods　A total of 100 patients with lumbar spondylolisthesis underwent surgical intervention were retrospectively analyzed,including 50 patients with mini-incision TLIF and 50 patients with conventional open opproach.Operation time, intra-operative bleeding, postoperative drainage volume, and hospitalization time were recorded. Pre- and postoperative back pain was assessed by visual analogue scale(VAS),and lumbar function was evaluated by Oswestry disability index(ODI),the clinical results were assessed by Macnab criterion,and radiologic parameters were compaerd.Results　The mean follow-up time was 18 months (ranged,12～24).The group of mini-incision TLIF was superior to the group of conventional open in intra-operative bleeding, operation time,postoperative drainage volume and hospital stay, postoperative VAS(P<0.01), the ODI in the patients with conventional open approach decreased from (85.9±3.9)% before operation to (19.2±4.9)% at the final postoperative follow-up.The ODI in the patients with mini-incision TLIF decreased from (84.4±5.3)% before operation to (7.7±1.3)% at the final postoperative follow-up.There was significant difference of the change in ODI between two groups(P<0.01).Conclusion　Compared with conventional approach, mini-incision TLIF meets the concept of minimally invasive surgery in treatment of lumbar spondylolisthesis, avoids wide stripping of the vertebral adjacent tissues,and has advantages of smaller incision,shorter hospitalization time,faster posteroperative recovery,and lower back pain and so no.It has defined clinical efficacy and simple operation,and is worth popularizing at the basic-level hospitals.]]> 2018/1/2 8:39:31 ZHAO Yong，QIU De-zan，HUANG Xiao-ying，et al. 613true ［Abstract］　Objective　To compare the effect, social function and safety of venlafaxine versus paroxetine in the treatment of general anxiety disorder.Methods　Sixty-eight patients with generalized anxiety disorder were randomly divided into two groups. The observation group was given venlafaxine, the control group was given paroxetine for 6 weeks. The Hamilton Anxiety Scale(HAMA),global assessment function(GAF) and side effects scale(TESS) were used to assess the therapeutic effects before treatment and at the end of the 1st,2nd,4th,6th week after treatment, respectively.Results　There was declined trend in HAMA′s total scores, mental anxiety scores and somatic anxiety scores in two groups. The MAMA′s total scores, mental anxiety scores at every time points after 1st week′s treatment and the HAMA′s somatic anxiety somatic anxiety scores at every time points after 2nd week′s treatment were significantly decreased in the observation group compared with those before treatment(P<0.05).The HAMA′s total scores, mental anxiety scores at every time points after 2nd week′s treatment, the HAMA′s somatic anxiety scores at every time points after 4th week′s treatment were significantly decreased in the control group compared with those before treatment(P<0.05).There was no statistically difference in the main effect of HAMA′s scores between two groups(P >0.05).The declined amplitude in HAMA scores in the observation group was significantly greater than that in the control group(P<0.05).There was an ascended trend in GAF′s scores in two groups. The GAF′s scores at every time points after 1st week′s treatment were significantly increased compared with those before treatment(P<0.05),but there were no statistically difference in the main effect of GAF′s scores and the ascended amplitude in GAF′s scores between two groups(P>0.05).There were no statistically difference in the rate of adverse reaction and the use of diazepams(P>0.05).Conclusion　Using venlafaxine and paroxetine for treating generalized anxiety disorder are effective and safe, but venlafaxine acts more rapidly and more improves the somatic symptoms compared with paroxetine.]]> 2018/1/2 8:39:31 ZENG Qiang,TAO Jian-qing,LIANG Jia,et al. 612true ［Abstract］　Objective　To observe the effect of Flipper mirror training accomodative facility on myopia developing in children.Methods　Sixty-two(117 eyes) myopia children aged 6 to 12 were divided into 3 groups according to the degree of myopia：Ⅰ group(<-3.00 D）；Ⅱ group（-3.00～-6.00 D）；Ⅲ group（>-6.00 D）. The changes in annual progression range of myopia after 6 months of Flipper mirror adjustment training were analyzed.Results　Compared with the original myopia，the progression range of myopia after Flipper mirror training significantly decreased(P<0.05)；there were statistically significant difference between different refractive power groups(P<0.05).Conclusion　Flipper mirror training accomodative facility can effectively delay myopic diopters developing in children，especially in more children with low to moderate myopia.]]> 2018/1/2 8:39:31 SONG Han，GAO Song，HAN Su-zhen，et al. 611true ［Abstract］　Objective　To explore the value transurethral catheterization of vas deferens and seminal veside in the diagnosis of seminal duct obstruction and the treatment of seminal duct diseases.Methods　A retrospective analysis was performed on the clinical data of 30 patients who received ureteroscopic retrograde cathe terication via ejaculatory duct and seminal veside and indwelling catheter for radiography,and treatment(including drug irrigation ect),in order to diagnose seminal duct obstruction and treat seminal duct diseases.Results　Angiography showed the seminal duct had obvious development in all patients. The patients had varying improvement in symptoms, Follow-up of 3 months showed 3 cases had recurrence.Conclusion　Transurethral catheterization of vas deferens and seminal veside in the diagnosis of seminal duct obstruction and treatment of seminal duct diseases has a good effect. Transureteroscopic catheterization of vas deferens and seminal veside has significant effect, fewer complications, and a high clinical value.]]> 2018/1/2 8:39:31 LIU Jun-ning, WANG Xiao-ping, ZHANG Wei-guo,et al. 610true ［Abstract］　Objective　To investigate the impact of blastocyst biopsy on the development potential and survival rate after vitrification of different quality blastocysts.Methods　The surplus embryos in IVF-ET cycles were allocated to good or poor quality blastocysts group by the embryo grade in day 5/6, and the same quality blastocysts were allocated to biopsy and control group. For the blastocysts from biopsy group trophectoderm removal was performed and then the survival blastocysts were vitrificated, but for control group vitrification was performed without biopsy. The survival rate after blastocyst biopsy and warming were compared between different groups.Results　For the poor quality blastocysts group the survival rate after blastocyst biopsy was lower than the good quality blastocysts group, but the difference was not significant(P=0.183), and the survival rate after warming was significantly lower than the good quality blastocysts group(P=0.001). But the survival rate after warming was similar between the biopsy group and control group（P=0.597，P=0.823）.Conclusion　Blastocyst biopsy did not impair the development potential and survival efficient after vitrification.]]> 2018/1/2 8:39:31 XUE Lin-tao, HUANG Li, HE Bing, et al. 609true ［Abstract］　Objective　To study whether there are differences in fetal pulmonary maturity and neonatal birth outcome between preterm infants in GDM pregnant women with normal blood glucose control under new diagnostic criteria and preterm infants in normal pregnant women.Methods　Ninety pregnant women with gestational age over 34 weeks, who had inevitable preterm labor or premature rupture of membranes and GDM corresponding to the new diagnostic criteria of GDM and had normal blood glucose control, were divided into 34 weeks group, 35 weeks group, 36 weeks group. Another 90 normal pregnant women as control group. The amniotic fluid was taken from viginal fornix or during membrane rupture on cesarean section for making foam test of amniotic fluid understanding of fetal pulmonary maturity. The comparison were performed of maternal age, mode of delivery, fetal pulmonary maturity, neonatal birth weight, neonatal asphyxia, neonatal pneumonia, neonatal hypoglycemia, NRDS index between two groups.Results　Between GDM group and control group there were no significant difference in the postpartum hemorrhage rate, cesarean section rate, fetal weight, placental weight, fetal pulmonary maturity and NRDS, neonatal asphyxia rate, neonatal hypoglycemia rate and neonatal infection rate.Conclusion　The fetal pulmonary maturity and neonatal birth outcome in preterm infants over 34 gestation weeks in GDM pregnant women with normal blood glucose control under new diagnostic criteria and those in preterm infants in normal pregnant women are same.]]> 2018/1/2 8:39:31 LONG Yu, TANG Hui, CHEN Yue, et al. 608true ［Abstract］　Objective　To research eye movement features and impact factors in visual search in patients with schizophrenia.Methods　One hundred and twenty schizophrenia patients were assigned to research group and 120 healthy volunteers to control group. Eye movement data were recorded by using Eyelink 2000 eye tracker when they were performing three tasks: searching for numbers, connecting the numbers by line and doing labyrinth games. Before the experiments, negative and negative symptoms were assessed using positive and negative syndrome scale(PANSS) in the research group.Results　In performing the three tasks, stimulating duration of searching for numbers, connecting the numbers by line and fixation time were significantly higher in the research group than in the control group (P<0.05 or <0.01), but the blink times in the task of doing labyrinth games was significantly lower in the research group than in the control group (P<0.05). Correlation analysis revealed that disturbance of attention factor of PANSS was positively related to the blink times in the task of doing labyrinth games, the response absence factor to times of saccade in the task of connecting the numbers by line, and the depressive factor to saccade amplitude when doing labyrinth games; paranoid factor was negatively correlated to the saccade amplitude when searching for numbers, recruitment factor to the saccade amplitude and stimulating duration and the total number of times of fixation in the task of connecting the numbers by line.Conclusion　Patients with schizophrenia showed anormaly eye movement features, thus revealing the possible damage to attention function, thinking flexibility, inference ability and problem-solving ability. These neurological damages may be associated with certain clinical symptoms, hint its relation to pathophysiological process of schizophrenia.]]> 2018/1/2 0:00:00 WANG Peng, CHENG zhe, HE Yi-qun. 607true ［Abstract］　Objective　To investigate the effects of lentivirus vector-Testis-specific Protein Y-encoded 1（TSPY1）on the proliferation ability of SMMC7721 and Huh7 hepatocellular carcinoma cells.Methods　The lentivirus vector was constructed and transfected into the SMMC7721 and Huh7 cells. The expression of TSPY1 mRNA and protein was detected by quantitative RT-PCR and western blot. Cell Counting Kit-8(CCK8) assay was performed to assess the transfected cells proliferation.Results　The expression of TSPY1 both in mRNA and protein levels were significantly increased in the SMMC7721 and Huh7 cells transfected with lentivirus encoding TSPY1 cDNA（P<0.01）. CCK8 assay indicated that TSPY1 potentiates cell proliferation both in SMMC7721 and Huh7 cells over-expressed TSPY1, comparing to the control cells.Conclusion　The transfection of lentiviralvector-mediated TSPY1 gene can obviously up-regulate the expression of TSPY1 mRNA and protein and promote the proliferation of SMMC7721 and Huh hepatocellular carcinoma cells.]]> 2018/1/2 9:44:23 ZHAI Li-min, MO Cui-ju, SUI Jing-zhe, et al. 606true ［Abstract］　Objective　To analyse the clinical pathologic factors predicting non-sentinel node(NSLN) involvement and develop a new model to quantify the risk of NSLN metastasis in sentinel lymph nodes(SLN)-positive breast cancer patients from Guangxi, in order to avoid unnecessary axillary lymph node disection(ALND) in low-risk patients with positive SLN.Methods　Eighty-three patients with positive sentinel lymph node biopsy(SLNB) followed by ALND were enrolled into our retrospective study.Univariate and multivariate analysis was used to identify variables predicting non-sentinel node involvement and a multivariable predictive model was developed. In order to assess the accuracy,new model was applied to the original series of 83 patients and the area under the receiver operating characteristic curve(AUC) was calculated.Distribution of continuous variables was analyzed using the Mann-Whitney U test, and the χ² test was used for categorical variables.Results　Size of the primary tumor, histological grade, lymphovascular invasion and size of SLN metastasis were revealed to be independent predictors of NSLN involvement in multivariate logistic regression and included in the final predictive model.For the original series of 83 patients, the AUC was 0.832(95%CI=0.744 to 0.919).Conclusion　In our study,new predictive tool was developed to assess the risk of additional axillary metastases after positive SLNB in breast cancer and represent considerable discrimination in our own population. but its accuracy needs to be assessed by prospective validation in both internal center and external centers.]]> 2018/1/2 9:44:23 LUO Ming，LI Fu，HE Liu-sheng，et al. 605true ［Abstract］　Objective　To design a adenovirus vector based system and specific siRNA for exploring the effects of overexpression and siRNA interference of the human hepatocellular carcinoma cell line HepG2 on the expression of 11β-hydroxysteroid dehydrogenase1.Methods　A adenovirus overexpression vector and specific siRNA targeting the 11β-HSD1 gene was designed and transfected into HepG2 cells. Expression of 11β-HSD1 mRNA and protein was analyzed by real time PCR and Western blotting.Results　A adenovirus transfection resulted in upgrade of 11β-HSD1 protein, and siRNA transfection led to inhibition of 11β-HSD1 mRNA and protein.Conclusion　Transfection of the hepatocellular carcinoma cell line HepG2 using a adenovirus vector and siRNA interference can significantly influence expression of 11β-HSD1 mRNA and protein.]]> 2018/1/2 9:44:23 SUI Jing-zhe, LI Guo-ping, LI Shan 604true ［Abstract］　Objective　To explore the curative effect of deciduous anterior teeth crossbite treated with fixed appliance.Methods　The curative effect of fixed appliance on deciduous anterior teeth crossbite in 15 children treated in orthodontics department of People′s Hospital of Guangxi Zhuang Autonomous Region from 2011 to 2014 were analyzed.Results　The longest curative course for crossbite to be quitted in 15 children was 2 months,the shortest was 3 weeks.Average total treatment course was (4±0.5)months. Deciduous anterior tooth overjet and overbite were normal after treatment.The curative effect of 13 children were stable following 2 years after treatment,and just 2 patients who had hereditary skeletal crossbite family history had a relapse.Conclusion　Treatment for deciduous anterior teeth crossbite with fixed appliance need little cooperation from the child,diminish child′s times of return visit,can quit crossbite smoothly,and was effective highly.]]> 2018/1/2 9:44:23 LU Shi-ying，TAN Gui-ping，HUANG Min-fang 603true ［Abstract］　Objective　To study the difference between the expression of lipopolysaccharide binding protein(LBP) in serum before and after mechanical ventilation, explore the specific marker in early diagnosis of ventilator-induced lung(VALI) injury and find out the target of clinical treatment.Methods　Twenty patients who received radical resection of lung cancer were selected. Every two lung cancer patients with the same surgical sites and methods were divided into the TLV group and the OLV group. Venous blood was collected at the moment before mechanical ventilation(T₀), at the end of operation(T₁),at 24 h after operation(T₂) and at 48 h after operation(T₃). After the blood was separated by centrifugation, the supernatant was collected and stored them at -80 ℃. Western blot method was used to measure the LBP expression levels.Results　The difference in the two groups was statistically significant at each time point(P<0.05), but the difference between the groups at each time point was not statistically significant(P>0.05), there was an interaction between the groups and each time points.The increased amplitude of LBP was different in the two groups,the OLV group had a bigger rise than the TLV group.Conclusion　Mechanical ventilation affects the LBP expression level, and the LBP indeed plays an important role in the VALI.]]> 2018/1/2 9:44:23 ZHANG Li-zhen，LI Yang，PENG Dan-hui，et al. 602true ［Abstract］　Objective　To explore the feasibility of low doses of gem energy spectrum CT colonography(CTC) in detecting polyps of the colon.Methods　A total of 50 patients with suspected colonic polyps underwent low-dose gem energy spectrum CTC and endoscopy. The colonic polyps were observed by endoscopy and gem energy spectrum CTC，with the endoscopic finding as gold standard. The coincidence was evaluated for colonic polyps by the gem energy spectrum CTC，and calculated CT dose index(CTDIw) in prone was recorded.Results　Thirty-eight patients were diagnosed to have colonic polyps by endoscopy and 12 were negative，the CTC negative diagnosis of colonic polyps in 16 cases and positive 34 cases. Total 79 polyps in 50 patients were found by CTC. For polyp size ≥0.5 cm and <0.5 cm, the compliance was 91.07%, and 50.00%，respectively.Conclusion　Reduction of the effective dose to 1.01 mGy significantly affects images quality on gemstone energy spectrum CTC，but the perception of ≥0.5 cm lesion not significantly impaired.]]> 2018/1/2 9:44:23 LI Jia-yan，HE Xin，HUANG Zeng-chao， et al. 601true ［Abstract］　Objective　To explore the situation of hospital medical personnel mastering the relevant knowledge about scientific and reasonable blood transfusion as well as saving blood transfusion within their respective jurisdictions.Methods　Some questionnaires designed by ourself were made for the 651 medical personnel in blood transfusion hospital for assessment before and after tutoring blood transfusion quality management and the qualification review, and did statistical analysis with the scores of the evaluation result and the accuracy of each answer.Results　Before counseling blood transfusion quality management and qualification review blood transfusion in level 2 and level 3 hospital nursing staff comprehensive score was （29.56±4.47） and （30.72±4.19） respectively; The doctors was （28.87±3.98） and （31.63±3.77） respectively; After counseling in level 2 and level 3 hospital, nursing staff comprehensive score was （39.19±1.20） and （38.40±1.54） respectively; Level 2 hospital′s nursing staff improved more than level 3 hospital′s. They got higher scores after counseling. There was statistically significant difference between the two(P all <0.05). In level 2 and level 3 hospital medical personnel′s comprehensive score was enhanced obviously, and it was （38.90±2.17） and （38.71±1.35） respectively. There was no statistically significant difference between the two groups(P>0.05). After review counseling training, in level 2 and level 3 hospital the situation of medical personnel mastering the knowledge of scientific and reasonable blood transfusion improved obviously. There was statistically significant difference between the two (P<0.05).Conclusion　In level 2 and level 3 hospital, after review counseling training, the situation of medical personnel mastering the knowledge of scientific and reasonable blood transfusion improves obviously, and different levels, nurse or doctor can master more blood transfusion knowledge. To carry out the quality management of blood transfusion and transfusion qualification review inspections can promote hospital strengthening the training of scientific and reasonable transfusion related knowledge, thereby promoting the hospital medical staff overall knowledge of reasonable blood transfusion.]]> 2018/1/2 9:44:23 CHEN Xing-zhi,YING Jian-min,WEI Hai-chun,et al. 600true ［Abstract］　Objective　To probe into the application effect of comprehensive rehabilitation training in the rehabilitation of preschool mentally retarded children.Methods　Thirty mentally retarded children were selected as the study objects to conduct comprehensive rehabilitation training in various fields such as language, cognition, fine motors, gross motors, and social adaptability, etc. for 6 months.Results　Developmental quotient of the 30 study objects was significantly improved after rehabilitation training compared with that before training (P<0.01).Conclusion　The comprehensive rehabilitation training mode in various fields is applied favorably in the rehabilitation of preschool mentally retarded children, which is worthy to be promoted.]]> 2018/1/2 9:44:23 ZHANG Ming-wu, MENG Jing-min, CHEN Guo-zhi, et al. 599true ［Abstract］　Objective　To understand the demographic data, HIV knowledge awareness and HIV, syphilis, HCV infection status for Vietnamese daughter-in-law in Ningming County, so as to provide the scientific basis for HIV prevention and control and behavior intervention for them.Methods　Using cluster sampling method to collect samples of Vietnamese daughter-in-law in 50 key villages/communities of Ningming county where Vietnamese daughter-in-law concentrated, face to face interview through questionnaire were conducted by trained staff, blood samples were collected to test HIV, syphilis and HCV. The survey data were inputed through Excel, analyzed by SPSS18.0.Results　Three hundred and sixty-three Vietnamese daughter-in-law were collected, their mean age was 18～65(38.97±9.02) years old, HIV knowledge awareness rate was 28.65%(104/363), The factors affecting HIV/AIDS knowledge awareness rate were the degree of culture and the way obtaining information of HIV/AIDS. HIV infection rate was 0.83%(3/363), the positive rate of syphilis was 1.93%(7/363), HCV infection rate was 0.Conclusion　The HIV/AIDS knowledge awareness of Vietnamese daughter-in-law is low. They are a notable importing HIV infection source group, and also the important group which needs education and surveillance for HIV/AIDS prevention and control.The HIV/AIDS education and publicity should be strengthened to people living in border rural areas, in the meanwhile, the premarital testing should be improved for those transnational marriage crowd.]]> 2018/1/2 9:44:23 XIANG Shao-mi,HUANG Hong-lang, NONG You-feng 598true ［Abstract］　Objective　To evaluate the application effect of gasless laparoscopic cholecystectomy using umbrella-like abdominal wall-lifting device in animal experiments.Methods　Twenty-seven pigs who received laparosopic cholecystectomy were randomly divided into two groups. One group was treated with gasless technique using umbrella-like abdominal wall-lifting device(umbrella group,n=15). Another group underwent pneumoperitoneum(gas group,n=12). The parameters were compared, including time of establishing operation space, time of gallbladder removal, bleeding volume and the unexpected occurrences during the operation between two groups.Results　All the surgeries were completed successfully without conversion to open procedure in two groups. The time of establishing operation space was no difference between two groups（P>0.05）. The greater omentum got stuck on the spokes during the process of opening the umbrella in two cases of umbrella group, there were no complications of massive hemorrhage and internal organ injury during the operations. There was no significant difference in unexpected occurrences between two groups(P=0.487).Conclusion　These exeperiment outcomes indicate that the umbrella-like abdominal wall-lifting device is feasible and safe in gasless laparoscopic cholecystectomy in animal experiments.]]> 2018/1/2 9:44:48 WU Dong-bo, SHAO Xin-hua, FENG Ze-rong, et al. 597true ［Abstract］　Objective　To observe the effects of right stellate ganglion block(SGB) on rat microcirculation during extracorporeal circulation.Methods　Twenty healthy SD rats were selected and randomly divided into two groups：control group（C group）and SGB group(RC group). Both C group and RC group were processed by extracorporeal circulation for 1 h.RC group received stellate ganglion block with bupivacaine before Undergoing cardiopulmonary bypass.The observation points included T0:before extracorporeal circulation；T1: after 30 min of extracorporeal circulation；T2: after 1 h of extracorporeal circulation.The observation included blood pressure, heart rate,blood gas parameters difference of oxygen pressure between artery and vein(Pa-vO₂) and blood lactate.Results　There were significant difference in lactate, Pa-vO₂ and blood pressure at different time between two groups(P<0.05). There was an interavtion effect in heart rate, lactate and Pa-vO₂ between treatment and time（P<0.05）. There were significant difference in the lactate and heart rate between two groups(P<0.05).Conclusion　Right stellate ganglion block can improve the tissue perfusion by ameliorating rat microcirculation during extracorporeal circulation.]]> 2018/1/2 9:44:48 HUANG Qian，ZHANG Bing-dong，WANG Ling 596true ［Abstract］　Objective　To investigate the adhesive effects of colorectal cancer cells SW480 by CLD1 gene transfection.Methods　Cell homotypic adhesive tests and cell-matrix adhesive tests were performed on SW480^CLD1(SW480 by CLD1 gene transfection),SW480^control(SW480 by pEGFP-C1 transfection) and SW480 cells.Results　The result of homotypic adhesive tests showed that the number of homotypic adhesive SW480^CLD1 cells were less than those of SW480^control（P=0.000）and SW480 cells（P=0.000）. No significant difference was detected between SW480^control and SW480 cells(P=0.376). The sequence of number of adhesive cell were SW480^CLD1control and SW480. The result of cell-matrix adhesive tests showed that the number of SW480^CLD1 adhesive cells were more than those of SW480^control(P=0.000) and SW480 cells(P=0.000), significant difference was detected. No significant difference was detected between SW480^control and SW480 cells(P=0.334). The sequence of number of adhesive cell were SW480^CLD1>SW480^control and SW480.Conclusion　The homotypic adhesion was decrease between SW480 cells by CLD1 gene transfection.The adhesion was increase between SW480 cells and collagen Ⅳmediated by CLD1 gene transfection.]]> 2018/1/2 9:44:48 CHEN Lin, WEI Ya-nan, LI Lin-mao,et al. 595true ［Abstract］　Objective　To observe toxic reactions in the experimental rabbits after skin touching the Zhuangyi Tonglu Medicinal Liquor.Methods　The experimental rabbits weighing (2.5±0.25)kg were used as testing animal，then randomly divided into groups and molded according to the half genders standard. Each experimental groups were smeared Zhuangyi Tonglu Medicinal Liquor onto the wound or the normal skin while the control group was smeared rice wine. Dermal reactions of different groups were observed.Results　The normal and wounded skin of rabbits in different groups showed no acute toxicity and long term toxicity, and Zhuangyi Tonglu Medicinal Liquor did not cause dermal irritation of rabbits. The experimental groups showed no significant difference compared with the control group(P>0.05).Conclusion　Treating arthralgia syndrome with Zhuangyi Tonglu Medicinal Liquor by the means of topical skin smearing has reasonable security.]]> 2018/1/2 9:44:48 ZENG Ping, LIANG Jing-chao, QIN Gang，et al. 594true ［Abstract］　Objective　To evaluate the clinical value and operative skills of transrectal ultrasound guided prostate biopsy by 6+X points method.Methods　Two hundred and ninety-two patients with histopathologic results underwent prostate biopsy guided by transrectal ultrasound and the postoperative complications were recorded.In 292 patients, prostate cancer were detected in 122 cases, benign prostate hyperplasia in 106 cases, chronic prostatitis in 28 cases, prostatic intraepithelial neoplasia in 18 cases, atypical hyperplasia in 15 cases,prostate tuberculosis in 2 cases, nonspecific granulomatous prostatitis in one case.Results　The detection rate of prostate cancer was 41.78%,the average puncture number was (6+1.73)times.After biopsy, the complications included:hematuria in 43 cases， bloody stool in 25 cases,fever in 20 cases，little hemorrhage in 20 cases, pain of back in 16 cases,urodynia in 14 cases,severe massive hemorrhage in one case.Conclusion　Transrectal ultrasound-guided prostate biopsy by 6+X points method has advantages of simple operation,less time consuming,less complications and high detection rate and has helpful for the early diagnosis and differential diagnosis of prostate cancer.]]> 2018/1/2 9:44:48 LIN Yun, WANG Xiao-yan, SHEN Gui-xin, et al. 593true ［Abstract］　Objective　To compare the difference among smart-care weaning, protocol- directed weaning and experience-based weaning in weaning patients with acute exacerbation of chronic obstructive pulmonary disease(AECOPD).Methods　Ninety-six patients with AECOPD receiving invasive mechanical ventilation,were selected and randomly divided into three groups.Results　Smart-care weaning method and protocol- directed weaning method were significantly shorter than experience-based weaning method in the duration of mechanical ventilation(MV)(P<0.01), the length of stay in the ICU（P<0.01）. And the incidence rate of ventilator associated pneumonia(VAP) were 18.8%, 25.0%, 46.9%,respectively,showing significant difference among three groups（P<0.05）. However, there was no significant difference in ICU mortality, extubation successful rate and rates of reintubation within 48 hours(all P>0.05).Conclusion　The smart-care weaning method and protocol- directed weaning method can shorten the duration of MV and the length of stay in the ICU, and reduce the incidence rate of VAP.]]> 2018/1/2 9:44:48 GUO Wei-yan，WANG Bao-hua，BAI Jing，et al. 592true ［Abstract］　Objective　To establish qualitative chemical fingerprint of Blumea riparia(BI.) DC. by high-performance liquid chromatography（HPLC）. Methods　Phenomenex® Synergi 4 u Polar-RP 80A（250 mm×4.6 mm，4 μm） column was used with 25 ℃ column temperature then gradient elution was performed by the mobile phase consisted of methanol-solution of acetic acid in water(pH 2.8) at 256 nm detecting wavelength with 1 ml/min flow rate. Determination of 12 batches of fingerprint chromatogram of Blumea riparia(BI.) DC. were performed, followed by using the computer-aided similarity evaluation system for similarity analysis of chromatograms.Results　Medicinal materials composed of 12 different batches were examined by specified method then we figured out its HPLC chemical fingerprint at 256 nm detection wavelength. Peaks in the chemical fingerprint of Blumea riparia(BI.) DC. were evaluatied and overall similarity index were analyzed and compared then 11 common peak were confirmed. This chemical fingerprint consist of 11 characteristic peaks including protocatechuic acid(the second peak) and chlorogenic acid peak(the fourth peak). According to similarity index able to reflect its medicinal properties, our 12 batches′s similarity index more than 0.99 so these 12 batches are high quality medicinal materials.Conclusion　The novel reliable quantitative chemical fingerprint analysis could be both foundation of quality assurance at industry level and be an important step for further application of Blumea riparia(BI.) DC.]]> 2018/1/2 0:00:00 LEELAKITTISIN Benjakarn, JIANG Wei-zhe, HUANG Xing-zhen 591true ［Abstract］　Objective　To explore the value of event related potential P300 in early diagnosis of vascular dementia.Methods　Inspection of P300 was performed between 50 cases of patients with vascular dementia(group of dementia), 49 arteriosclerosis patients without dementia(group of arteriosclerosis) and 48 healthy people(group of healthy people).Results　The P300 incubation period of the group of dementia was prolonged significantly and amplitude decreased compared with the group of arteriosclerosis and the group of healthy people. The difference was statistically significant between the compared groups(P<0. 01).Conclusion　Event related potential checking has higher application value in early diagnosis of vascular dementia.]]> 2018/1/2 9:44:48 HONG Yan, LIANG Zhi, FANG Fang, et al. 590true ［Abstract］　Objective　To explore the gender differences in risk factors of hypertension in town residents for making gender-differentiated strategies of hypertension prevention in general population.Methods　One thousand and fifty-seven adult residents were enrolled by two stage stratified cluster sampling, and the univariate and multivariate logistic regression were used sequentially to estimate the risk factors of hypertension. All analyses stratified by sex were performed.Results　Three hundred and forty-seven participants with hypertension were detected, 107 men and 240 women, the proportions of male and female were 30.84% and 69.16%, respectively. Multivariate logistic regression analysis showed that older age(OR=1.045, 95%CI:1.011～1.063), lack of physical activity(OR=4.596,95%CI:1.657～12.734), and smoking(OR=1.058,95%CI:1.073～1.524) were associated with hypertension of men. For women, older age(OR=1.074,95%CI:1.052～1.091), menopause(OR=2.022,95%CI:1.082～3.775), eating organ meats(OR=1.043,95%CI:1.009～1.054), BMI≥24 kg/m²(OR=2.204,95%CI:1.641～2.973), and diabetes(OR=2.122,95%CI:1.313～3.442) were associated with hypertension.Conclusion　Gender differences in risk factors of hypertension were significant in town population, and this suggests that gender-differentiated strategies are needed while preventing hypertension in general population.]]> 2018/1/2 9:44:48 HU Jin-lan, LI Xiao-fei, LONG Ying-quan, et al. 589true ［Abstract］　Objective　To investigate the diagnostic value of the measurement of CA125 in pleural effusion for tuberculous pleural effusion.Methods　Thirty confirmed tuberculous pleurisy patients who received the measurement CA125 of pleural effusion from January 2008 to January 2014 were pooled into observation group. The control group 30 patients were made up of patients with parapneumonic effusion or autoimmune disease patients with pleural effusion. Measurement of CA125 in all patients′ pleural effusion was performed by immunochemical fluorescent assay. Intergroup statistics analysis had been carried on.Results　The observation group showed CA125<68.6 U/ml in 3 patients，68.6 U/ml≤CA125≤600 U/ml in 17，>600 U/ml in 10. The control group showed CA125<68.6 U/ml in one patient，68.6 U/ml≤CA125≤600 U/ml in 28，>600 U/ml in 1. There was statistically significance between two groups（P<0.05）.Conclusion　Compared with parapneumonic effusion and autoimmune-associated pleural effusion, concentrations of CA125 in tuberculous pleural effusion have a tendency inclination of higher level. Furthermore, the measurement of CA125 in pleural effusion appears to be beneficial for diagnosing tuberculous pleural effusion in non-malignant pleural effusion patients.]]> 2018/1/2 9:44:48 LUO Ling，QIN Xue-jun，LU Ai-ling，et al. 588true ［Abstract］　Objective　To investigate the level of Th22 cells and IL-22 in peripheral blood of patients with hepatocellular carcinoma(HCC) and their correlation with tumor markers.Methods　Th22 cells in blood samples from 45 patients with HCC and 15 normal controls were detected by flow cytometry(FCM). IL-22 levels were detected by an enzyme-linked immunosorbent assay. Statistical analysis of the relevance of Th22 cells, IL-22 levels and tumor markers including AFP, CA125, CA193, CA199, CEA was performed.Results　The level of Th22 cells and IL-22 in peripheral blood of patients with primary hepatic carcinoma were significantly higher than that of normal controls(P<0.05), but no significant between male and female group. The percentage of Th22 cells in HCC patients had positive correlation with the level of IL-22. Th22 cells, IL-22 levels had no correlation with tumor markers including AFP, CA125, CA193, CA199, CEA(P>0.05).Conclusion　The levels of Th22 cells and IL-22 expression in peripheral blood of HCC patients are high but have no correlation with tumor markers and their clinical significance in HCC still need large clinical sample analysis.]]> 2018/1/8 8:50:20 MA Shi-jia, GUO Xiao-yun, TAO Lin, et al. 587true ［Abstract］　Objective　To investigate the expression of CC chemokine receptor 9(CCR9) on peripheral blood CD4⁺ T lymphocyte cells of non small cell lung cancer(NSCLC)， and the effect of CCR9 and its ligand(CCL25) on peripheral blood CD4⁺ T lymphocytes.Methods　The study was performed in 42 NSCLC patients and 30 healthy controls. Flow cytometry was employed to detect the T lymphocyte subsets and the expression of CCR9 on peripheral blood CD4⁺ T cells. Peripheral blood CD4⁺ T lymphocytes were isolated from all cases with magnetic-activated cell sorting method. The cell trans-membrane test with 24-transwell was used to detect the effect of CCR9/CCL25 on the lymphocyte migration.Results　Compared with control group, the T lymphocyte subsets in pre-operative patients were higher than that in post-operative patients(P<0.05). The frequency of CD4⁺CCR9⁺ T lymphocyte in pre-operative group was both significantly decreased compared with post-operative group(3.33±1.11 vs 6.57±1.92，P<0.05) and controls(3.33±1.11 vs 11.06±1.37，P<0.05). In the induce of CCL25, the chemotactic indexes(CI) of CD4⁺ T lymphocytes from NSCLC patients(3.14) was significantly lower than that from healthy persons(3.83). After anti-CCR9 mAb pretreatment, the lymphocytes CIs of NSCLC patients decreased significantly to 0.62 as compared with untreated controls.Conclusion　The expression of CCR9 is down-regulated on NSCLC peripheral blood CD4⁺ T lymphocyte cells. CCR9/CCL25 interactions mediate the migration of CD4⁺ T lymphocytes in bodies. The decreased expression of CCR9 in the CD4⁺ T lymphocytes of patients with NSCLC could effect the ability of lymphocytes migration, which might involve in the mechanisms of tumor escape immune surveillance. Operation could reverse the change of CCR9 expression on CD4⁺ T lymphocyte cells. CCR9 may be suggested to indicate immunologic reconstitution after therapy and operation.]]> 2018/1/8 8:50:20 ZHONG Yong-long, LIN Hui, LAN Jiao, et al. 586true ［Abstract］　Objective　To prepare of acetyl resveratrol and establish a high performance liquid chromatography(HPLC) method for its content determination.Methods　Resveratrol and acetic anhydride as raw materials,the reaction temperature was 120 ℃.The inertsil ODS-SP column(150 mm×4.5 mm,5 μm) was used and the mobile phase was CH₃CN-H₂O(45∶55,V/V),the flow rate was 1.0 ml/min.The detection wavelength was 300 nm.The column temperature was 25 ℃ and the injective volume was 20 μl.Results　The reaction get a high purity of acetyl resveratrol,the yield was 90%.A good linear relationship was observed in the concentration range of 20.0～400.0 μg/ml,with the correlation coefficient of 0.999 9.The average recovery rate was 99.82% and RSD was 0.89%(n=6).Conclusion　The synthetic method is proved to be simple and the determination method was accurate and sensitive with high precision,which can be applicable for the preparation of acetyl resveratrol and its content assay.]]> 2018/1/8 8:50:20 HUAGN Shao-de, WEI Gui-yun, LIANG Kai, et al. 585true ［Abstract］　Objective　To evaluate the outcome of the patients with skeletal class Ⅱ malocclusions treated by extraction or non-extraction through the occlusions assessing.Methods　The post-treatment dental casts of two groups of adolescence patients with skeletal class Ⅱ malocclusions treated by extraction or non-extraction respectively were collected. The PAR index were measured on dental casts quantitatively. The PAR score were analyzed to compare the dental-occlusion difference between the two groups.Results　After treatment, the weighted overall PAR scores was （0.62±0.74） in extraction group and （0.90±1.37） in non-extraction group and there was no significantly different between two groups(P>0.05).The other PAR scores were all low and showed no statistical differences between two groups(P>0.05).Conclusion　Evaluating from the dental-occlusion,both extraction and non-extraction treatment can achieve successful results.]]> 2018/1/8 8:50:20 CHEN Qiu-lan, FANG Zhi-xin, ZHOU Yan, et al. 584true ［Abstract］　Objective　To compare the clinical effects of double ENDOBUTTON CL plate versus clavicular hook-plate in the treatment of Tossy Ⅲ type dislocation of acromioclavicular articulation.Methods　Sixty-seven patients with Tossy Ⅲ type acromioclavieular dislocation were treated with open reduction and internal fixation. Out of the 67 patients, 32 patients were treated with clavicular hook, 35 patients with ENDOBUTTON CL system. The outcomes were graded into excellent, good, fair and poor according to the Constant CR criterion.Results　All 67 patients were followed up from 8 to 28 months［averaged （12.4±2.6）months］. All acromioclavicular joint dislocation was reduced. All patients recovered from abnormal function and no complication happened. According to the scales of Constant CR, the post-operation shoulder-joint function were excellent in 11 patients, good in 9 patients, fair in 7 patients and poor in 5 patients in the clavicular hook group. The rate of both excellent and good were 62.5%(20/32); the post-operation shoulder-joint function were excellent in 16 patients, good in 14 patients, fair in 4 patients and poor in 1 patient in the ENDOBUTTON CL System group. The rate of both excellent and good were 87.7%(30/35). The difference of the rate of both excellent and good had statistical significance between the two groups(χ²=4.7577，P<0.05).Conclusion　In the treatment of Tossy Ⅲ acromioclavicular dislocation, fixation of double ENDOBUTTON CL plate is better than hook plate fixation.]]> 2018/1/8 8:50:20 ZHU Jiang, LIU Wen-he, LI Yun-hua, et al. 583true ［Abstract］　Objective　To investigate the relationship between HPV infection and chronic urticaria(CU).Methods　HPV-IgG was detected by ELISA assay in 100 patients with CU and 100 healthy controls.Results　The positive rate of HPV-IgG was 28% in CU group and 12% in healthy control group, showing significant difference between two groups(χ²=8.000，P=0.005).Conclusion　There is a correlation between chronic urticaria and HPV infection, HPV infection may be one of the pathogenesis of CU.]]> 2018/1/8 8:50:20 HU Ji-rong, LI De-xian, LUO Fang-ting，et al. 582true ［Abstract］　Objective　To optimize the extraction process of love mouth gargle liquid, ensure the quality of preparation.Methods　Taking three factors(water volume, extracting time and extracting times) as investigation factors, with three indexes(berberine hydrochloride content, total polysaccharide content and extract yield), the optimum extraction process of the love mouth gargle liquid was examined using the comprehensive evaluation method.Results　The optimum extraction process for preparation included adding 6 times amount of water, extraction time of 30 minutes, 3 times of extraction.Conclusion　The optimum extraction process is feasible, good stability, can effectively guarantee the quality of preparation.]]> 2018/1/8 8:50:20 LI Jing, ZHENG Pei-qiu, MO Xiao-lin 581true ［Abstract］　Objective　To explore the effect of digital acupoint pressure therapy in the treatment of knee osteoarthritis(KOA).Methods　There were totally 46 knee joint with KOA in 30 patients, in which there were 16 patients with 32 KOA knees, and 14 patients with 14 KOA knees. All of the patients accepted digital acupoint pressure therapy. The knee joint effusion was measured with color Doppler ultrasonography before and 3 months after treatment, and the severity of KOA was assessed with KOA severity index(ISOA).Results　In the thirty patients with 46 KOA knees, after accepting the pointing treatment for 3 months, the ISOA score was obviously decreased compared to that before the treatment, the difference was statistically significant(t=15.652, P=0.000). Color Doppler ultrasonography showed that the diameter of effusion in knee joint cavity decreased significantly(t=3.446, P=0.001), however, the reduction of the number of effusion joint, compared to those before treatment, was not statistically significant(χ²=1.848, P=0.257).Conclusion　The digital acupoint pressure can effectively release or eliminate the clinical symptoms of KOA.]]> 2018/1/8 8:50:20 LV Ya-nan, LI Rong-zhu, WANG Xiao-yan, et al. 580true ［Abstract］　Objective　To observe the proliferation and apoptosis of the lipopolysaccharide (LPS)-induced HaCaT cells in the presence of the active leflunomide metabolite A771726.Methods　MTT was used to detect the level of cell proliferation; flow cytometry (FCM) was used to detect apoptosis of HaCaT cells; inverted phase contrast microscope (IPCM) was used to observe morphologic changes.Results　The A771726 significantly inhibited the proliferation of LPS-induced HaCaT cells, the inhibition effect was concentration-and-time dependent; the A771726 promoted cell apoptosis and the apoptosis rate increased with its concentration, the LPS group showed dense monolayer cells under an inverted microscope, as the concentration of the A771726 increased, the volume of the cells got smaller and intercellular space got larger, and the more vacuoles was observed in the cells.Conclusion　Leflunomide can inhibit the proliferation of the LPS induced HaCaT cells and expedite their apoptosis, which may be one of the mechanisms of leflunomide in the treatment of psoriasis.]]> 2018/1/8 8:50:20 CHENG Qiu-mei, PAN Yan-bin, TAN Mei-le,et al. 579true ［Abstract］　Objective　To compare serum protein pattern of patients with liver cancer from high-risk families for liver cancer with that of patients with liver cancer from no-risk familial pedigree for liver cancer and detect specific biomarkers in serum between two groups.Methods　SELDI-TOF-MS Protein Chip was used to detect serum protein patterns of 25 patients with liver cancer from high-risk familial pedigree for liver cancer and 25 patients with liver cancer from no-risk families for liver cancer. Diagnostic model was developed and validated by using BPS.Results　At the molecular weight range from 2 000 to 50 000，15 protein peaks were obviously different between two groups.Analyzed by BPS, the diagnostic model′s accuracy was 92.0%（46/50）,sensitivity was 96.0%(24/25) and specificity was 88.0%（22/25）.Conclusion　There are amount of the special serum differential proteins between two groups,SELDI-TOF-MS Protein Chip array technology has better clinical value in detecting the special serum protein biomarkers.]]> 2018/1/8 8:50:54 DENG Jing-huan, CHEN Zhi-ping, LI Shan, et al. 578true ［Abstract］　Objective　To compare the dose volume histogram(DVH) parameters and dynamic electrocardiogram(DCG) changes between intensity modulated radiotherapy(IMRT) and conventional radiotherapy(CRT) for the thoracic tumor.Methods　The patients with thoracic tumors were divided into IMRT group and CRT group,with 36 cases in every group, two groups were tested with 24-hour DCG before, during and after treatment to compare DVH parameters and DCG changed.Results　Heart V₃₀-V₅₀ in IMRT group were significantly lower than that in the CRT group, with statistical significance（P<0.01）, the abnormity incidence rates of DCG was statistically significant between IMRT group and CRT group(before, during and after treatment)(P<0.01), the abnormity incidence rates of DCG were lower in IMRT group than that of CRT group during and after treatment, with statistical significance（P<0.05）.Conclusion　Both treatments can cause DCG change, but at the same dose IMRT group has smaller heart irradiation volume and lighter change of DCG.]]> 2018/1/8 8:50:54 YANG Su-ping, QIN Xiao-bo, HAO Yan-rong, et al. 577true ［Abstract］　Objective　To assess the effect of non-processed sperm parameters and processed sperm parameters on pregnancy outcome of husband sperm of intrauterine insemination（IUI）.Methods　A retrospective study was performed on 530 IUI cycles in our center. The cycles were divided in to 3 groups according to the processed(or non-processed) progressive motile sperm count or sperm recovery rate. The clinical pregnancy rates among different groups were compared.Results　（1）According to the non-processed progressive motile sperm count, the cycles were divided into 3 groups:<20×10⁶/ml, >20～<100×10⁶/ml and ≥100×10⁶/ml, the clinical pregnancy rates were 10.08%, 12.79% and 12.50% respectively, there were no significant differences among 3 groups(P>0.05); （2）According to the processed progressive motile sperm count the cycles were divided into 3 groups:<10×10⁶/ml, >10～<20×10⁶/ml and ≥20×10⁶/ml, the clinical pregnancy rates were 8.42%, 10.59% and 12.57% respectively, there were no significant differences among 3 groups(P>0.05); （3）According to sperm recovery rate, the cycles were divided into 2 groups: <25% and ≥25%, the clinical pregnancy rates were 10.45% and 12.57% respectively, there were no significant differences between two groups(P>0.05).Conclusion　With the increase of non-processed or processed progressive motile sperm count and sperm recovery rates, the IUI pregnancy rates gradually increase, but there were no statistically difference.]]> 2018/1/8 8:50:54 WANG Shi-kai，HUANG Li，QIN Jie，et al. 576true ［Abstract］　Objective　To know the bacterial distribution on surgical incision and antimicrobial application in primary hospital and provide the basis for clinical antibacterial drug application.Methods　Bacterial culture was performed in 246 patients′ surgical incision treated in primary hospital by single blind, and recorded antimicrobial application in perioperative period,then compared and analyzed it with related regulations and reference from Chinese medical association and national hygiene department.Results　Seventy-nine strains of pathogenic bacteria had been detected in 246 patients′ surgical incision, including 53 strains of E.coli(67.09%), 24 strains of Staphylococcus(30.38%) and 2 strains of pseudomonas aeruginosa(2.53%).The 246 patients used 6 class，14 kinds of antibacterial drugs，417 times without considering the surgical incision category, the usage rate of antibacterial drug was 100%,second generation cephalosporin was the most frequently used(50.60%), there were problems of without indications drugs,most of the perioperative use of antimicrobial medicine, chaotic medicine choices, too long medication,inaccurate medication time, and improper carrier solvent existed in the perioperative period of most antimicrobial application.Conclusion　The main gram positive bacteria of surgical incision is epidermis staphylococcus in primary hospital;the antimicrobial application in perioperative period at present is still generally unreasonable, more effective intervention and supervision measures should be adopted,in order to further promote the reasonable antimicrobial application in clinic.]]> 2018/1/8 8:50:54 FAN Xian-wei，LIANG Ai-chan, LUO Hai-hui, et al. 575true ［Abstract］　Objective　To explore the histological changes in intrinsic muscle and the neuropathological changes in anastomosis during repairing high ulnar nerve injury(above the elbow) at the first stage using sensory nerve transferring by experiments conducted with animals.Methods　Six adult male macaques were included in the experiment and the upper limbs were studied, with the 12 limbs randomized into 3 groups(4 limbs in each group).Group A(experimental group): The ulnar nerve was cut at the upper segment of the upper limb, with end-to-end anastomosis followed. The superficial branch of radial nerve was cut at the distal end and the end-to-side anastomosis was performed by transferring the superficial branch of radial nerve to the wrist segment(fenestration was made in the outer membrane).Group B(control group): 3 cm of the ulnar nerve was cut at the upper segment of the upper limb and folding ligation was made from both ends.Treatment at the wrist segment was the same as in group A. Group C(Control group): Treatment at the unlar nerve of the upper was the same as in group A. But no transferring was made at the wrist segment. The atrophy of intrinsic muscle controlled by ulnar nerve was observed after surgery. The intrinsic muscle at the end-to-side anastomosis, neural stem distal to end-to-side anastomosis and hypothenar muscle were collected at 1M, 3M, 6M and 12M after surgery and the tissues collected were made into sections to observe the changes under microscope.Results　The intrinsic muscle of the macaques in group A restored autonomic activity 12 months after surgery and no obvious atrophy in intrinsic muscle of the operated side was observed. Atrophy in intrinsic muscle of the operated side was observed in group B and group C, but with more obvious atrophy observed in group C. Histological observation revealed that the number and density of nerve fibres increased as the time went on in group A. While in group B, the number and density of nerve fibres kept stable after increase to certain degree, but no necrosis was observed. In group C, the number of nerve fibres decreased significantly as time went on. The number of muscle fibres also decreased significantly and besides atrophy, hyalinization, interstitial hemorrhage and formation of granulation tissue were observed in most muscle fibres.Conclusion　Sensory nerve transferring is effective in preventing atrophy, degeneration and fibrosis of intrinsic muscle and provides favourite situation for the regeneration and growth of ulnar nerve after repairing for high injury.]]> 2018/1/8 8:50:54 LIANG Bin， LI Rong-zhu，WEI Shao-ren，et al. 574true ［Abstract］　Objective　To investigate the proportion and diagnostic techniques of pulmonary mixed ground-glass opacity(mGGO), and the relationship between the disease and computed tomography(CT) characteristics.Methods　Single mGGO were found in 22 consecutive patients which underwent multi-detector CT. Nineteen(86.4%) consecutive cases of mGGO nodules that were histologically confirmed were included in this prospective trial. All cases of pulmonary mGGO nodules were divided into two groups: malignant nodules group and benign nodules group. The clinical and radiological characteristics(including the lesions′ size, edge characteristics, internal characteristics, etc) of all patients were recorded. These parameters were statistically compared between the two groups.Results　There were 4 benign and 15 malignant mGGOs. No statistical differences were found between benign and malignant mGGOs in terms of demographic data, lesions′ size, internal characteristics and external manifestations. The frequency of lobulation(86.7%), well-defined interface（86.7%） and vascular convergence（80.0%）was significantly higher in malignant mGGOs than that in benign mGGOs. The percentage of GGO component ≤50% was higher in malignant mGGOs. The diagnostic of 11 cases were confirmed through percutaneous core needle lung biopsy under multi-CT fluoroscopic guidance.Conclusion　The CT characteristics of pulmonary mGGO nodules have certain significance in the differential diagnosis of lesions properties of pulmonary mGGO nodules. CT fluoroscopy-guided needle aspiration biopsy is a useful diagnostic technique for mGGO nodules.]]> 2018/1/8 8:50:54 JIANG Ling-yu, QIN Zhi-qiang, WANG Yi, et al. 573true ［Abstract］　Objective　To investigate whether or not the abnormal blood lipid and inflammatory reaction associated with carotid atherosclerotic plaque formation in patients with coronary heart disease.Methods　One hundred and thirty-two coronary heart disease patients with or without carotid atherosclerotic plaque hospitalized in our hospital from May 2009 to April 2013 were enrolled in this study, including 93 patients with carotid atherosclerotic plaque(observation group) and 39 patients without carotid atherosclerotic plaque(control group). Total cholesterol（TC）, low density lipoprotein cholesterol（LDL-C）, triglyceride（TG）, high density lipoprote cholesterol（HDL-C） and hypersensitive C-reactive protein(hs-CRP) in all patients were detected routinely.Results　The observation group had higher TC and higher LDL-C than the control group（P<0.05 and P<0.01, respectively）. However，TG, HDL-C and hs-CRP between two groups had not statistically significant difference（P all >0.05）.Conclusion　In patients with coronary heart disease, higher TC and higher LDL-C may be related with the formation of carotid plaque，while hs-CRP has not apparently related with the formation of carotid plaque.]]> 2018/1/8 8:50:54 LU Zhi-dong, PAN Qing-deng, HUANG Yong-kang 572true ［Abstract］　Objective　To investigate the protein expression of Breast cancer1(BRCA1) in nasopharyngeal carcinoma(NPC) and to analyze the relationship of BRCA1 expression with the prognosis.Methods　The expression of BRCA1 proteins in the tissue of nasopharyngeal carcinoma in 100 patients with NPC was detected by immunohistochemistry. The high BRCA1 expression group included patients whose biopsy specimens showed at least 50% immunopositive tumor cells; The low BRCA1 expression group included patients whose biopsy specimens showed less than 50% immunopositive tumor cells, including negative expression.The correlations between protein expression and clinical data were assessed using the chi-square test.Results　Thirteen patients failed to be followed-up. And in the rest 87 patients, the high BRCA1 expression group and low BRCA1 expression group included 37 patients(42.5%) and 50 patients(57.5%) respectively. No significant differences were found between the expression of BRCA1 proteins and TNM stage(P=0.383), tumor regression(P=0.434), or distant metastasis(P=0.055). The survival rate in the high BRCA1 expression group was higher than that in the low BRCA1 expression group, but there was no statistically significant differences between two groups(P>0.05).Conclusion　There was no significant correlation between the expression level of BRCA1 and TNM stage and tumor regression in nasopharyngeal carcinoma. However,the low expression of BRCA1 were more prone to metastasis tendency.]]> 2018/1/15 8:41:55 YANG Jiao, HAO Yan-rong, FENG Guo-sheng, et al. 571true ［Abstract］　Objective　To investigate the change trend of T lymphocyte subsets(CD3⁺CD4⁺,CD3⁺CD8⁺,CD4/CD8) and regulatory T cells(Treg, CD4⁺CD25^+highCD127^low) in adult patients with steroidresistant nephrotic syndrome(SRNS) and its clinical significance.Methods　Twentyfour SRNS patients enrolled in SRNS group were chosen from 65 inpatients with primary nephrotic syndrome(PNS) first treated with prednisone(1 mg·kg^-1·d^-1) for eight weeks, according to the state of clinical remission of PNS. Thirty normal persons were served as healthy control group. T lymphocyte subsets and Treg were detected by flow cytometry before treatment(W0 group), after 4week treatment(W4 group) and after 8week treatment(W8 group).Results　①Compared with W0 group, plasma albumin(Alb) increased significantly(P<005), while urinary protein, serum creatinine(Scr), blood urea nitrogen(BUN), cholesterol(Cho)and triglycerides(TG) had no significant differences in W8 group（P>005）.②Compared with control group, CD4% and CD4/CD8 ratio increased significantly, while Treg/CD4 ratio decreased significantly in W0 group(P<005).Compared with W0 group,CD4%,CD8%,CD4/CD8,Treg and Treg/CD4 had no significant differences in W8 group(P>005).Conclusion　There are changes of T cell populations, T lymphocyte subsets and Treg/CD4 ratio imbalance in SRNS adult patients. The clinical manifestation and the disorder immune state even sustained after 8week treatment of glucocorticoid.]]> 2018/1/15 8:41:55 YE Kun, WANG Haoyu，GONG ZhiFeng, et al. 570true ［Abstract］　Objective　To investigate of the establishment of mild hypothermic cardiopulmonary bypass(CPB) on beating heart for Guangxi bama miniature pig.Methods　Thirteen bama pigs weighted 32～40 kg were chosen,3 pigs were used for sampling blood,another 10 pigs were used for cardiopulmonary bypass.Pigs underwent chest incision under general anesthesia.The superior vena cava and the inferior vena cava were circumferentially mobilized and encircled with ties outside the pericadium to establish CPB,blocking superior and inferior vena cava cannulas without cross-clamping the aorta.The heart rate was maintained 50 to 80 bpm,the nasopharyngeal temperature was kept at 32～34 ℃,flow volumes were 80～100 ml·kg^-1·h^-1,mean arterial blood pressure were 60～80 mmHg,HCT were 20%～25% during CPB procedure.Results　One pig was died of hypoxia induced intubation to esophagus. One pig loss excessive blood when disassociated the inferior vena cave. One pig appeared transient ventricular fibrillation for 4s when CPB initiated.Esmolol was used when HR over 80bpm to induce adequate bradycardia effect.In two cases mild hemoglobinuria was experienced.Conclusion　Establishment of mild hypothermic cardiopulmonary bypass on beating heart for Guangxi bama miniature pig was a ideal model for cardiac surgery on beating heart under CPB.]]> 2018/1/15 8:41:55 HU Yan-yan,LIN Hui,HUANG Ai-lan,et al. 569true ［Abstract］　Objective　To investigate the relationship between tumor necrosis factor-α（TNF-α）,Interleukin-1β（IL-1β）,Osteoprotegerin（OPG）and type 2 diabetes mellitus（T2DM） with coronary artery disease(CAD).Methods　Thirty healthy individuals（control group）, and 30 T2DM patients with CAD(T2DM+CAD) group were selected. ELISA was used for determination of TNF-α，IL-1β and OPG，biochemistry method for blood glucose and glycosylated hemoglobin, and the data in all groups and the relationship between the factors was observed.Results　Fasting blood-glucose, glycosylated hemoglobin， OPG in T2DM group were obviously higher than those in control group（P<0.01）,and TNF-α,IL-1β in T2DM group were obviously higher than those in control group（P<0.05）.Spearman correlation analysis showed that the positive correlation existed between OPG and TNF-α,IL-1β(P<0.01）.Conclusion　The levels of blood glucose，glycosylated hemoglobin，TNF-α,IL-1β and OPG in T2MD group increase obviously. The level of OPG was related to the changes of TNF-α and IL-1β.]]> 2018/1/15 8:41:55 LU Wei-jun，CHEN Hui, LIU Ling，et al. 568true ［Abstract］　Objective　To analyze the clinical characteristics and antimicrobial susceptibility of pneumonia infected with Escherichia coli in infants for clinical pneumonia treatment.Methods　Retrospective analysis was performed for 177 cases of pneumonia infected with Escherichia coli in hospitalized infants with clinical manifestations, X-ray findings, bacterial drug resistance and treatment outcome.Results　The main symptoms of clinical manifestations in infants of pneumonia infected with Escherichia coli were cough, sputum, fever, and likely to prone to breathing difficulties. X-ray mainly showed patchy or interstitial exudation. The drug sensitivity test showed that Escherichia coli were sensitive to tienam, mei-ping, ceftazidime, amikacin，levofloxacin, piperacillin/tazobacta and cefoperazone/sulbactam. While they were resistant to most β-lactam antibiotics. There were 33 cases of pneumonia of Escherichia coli with producing extended-spectrum β-lactamase(ESBLs) bacteria with 18.6% positive rate. The negative conversion sputum culture of Escherichia coli in pneumonia infants was relatively slow. Treatment for pneumonia infected with Escherichia coli in infants was mainly empirical therapy combined with drug susceptibility test results according to sputum culture of Escherichia coli, while the prognosis of pneumonia infected with Escherichia coli was still good although prolonged course.Conclusion　Escherichia coli pneumonia in infants was a common pediatric diseases, which clinical diagnosis relatively easy. However, there be likely needed to pay attention to higher antimicrobial resistance to Escherichia coli.]]> 2018/1/15 8:41:55 XIE Qing-ling, TANG Xiao-yan, ZHEN Hong, et al. 567true ［Abstract］　Objective　To study the condition and influencing factors associated with diagnosis delay for patients with new smear-positive pulmonary tuberculosis, in order to provide a reference for tuberculosis control work in Guangxi.Methods　One huandred and eighty patients with new smear-positive pulmonary tuberculosis reported in 2011were selected from 4 units of different tuberculosis control mode, and the data of medical record information were collected for statistical analysis.Results　The diagnosis delayed rate was 44.4% in patients with new smear-positive pulmonary tuberculosis, the median time of diagnosis was 19 days. Logistic regression analysis showed that patients with the gender of men(OR=3.502, P=0.006) and came from the unit of CDC control mode(OR=2.797, P=0.032) had higher risk of diagnosis delay.Conclusion　It was useful to strengthen the tuberculosis health education，develop measures of benefit to the people，and gradually implement transformation of the tuberculosis control mode，for reducing the happening of diagnosis delay in Guangxi.]]> 2018/1/15 8:41:55 WU Teng-yan，LIU Fei-ying，HUANG Shu-hai，et al. 566true ［Abstract］　Objective　To investigate the status of dietary nutrients intake of the elderly patients with metabolic syndrome in Yinchuan city, and provide the advices for the prevention of metabolic syndrome.Methods　One hundred and twenty elderly patients with metabolic syndrome come from 4 communities in Yinchuan were investigated by general population sociology questionnaire and food frequency questionnaire.Results　Among these elderly patients with metabolic syndrome, the intake of oil and salt was excessive and the fresh fruits intake was not enough. In addition, carbohydrate energy was not enough and the fat energy was excessive.Conclusion　The dietary nutrients intake of the elderly patients with metabolic syndrome in Yinchuan was unreasonable. So their dietary pattern should be adjusted to reduce the intake of salt and fat and increase the intake of fruits and vegetables.]]> 2018/1/15 8:41:55 NING Yan-hua, ZHANG Lin, GU Bo-rong, et al. 565true ［Abstract］　Objective　To study the expression of lung resistance protein(LRP) in non-small cell Lung Cancer(NSCLC) and its correlation with Platium resistance and prognosis.Methods　The expression of LRP in 89 NSCLC patients was detected by the way of immunohistochemical method. The relationship between LRP and clinicopathological feature and the others(age, sex, smoking history, histological type, differentiation, distant metastasis, lymphatic metastasis and so on) were studied.Results　In the tissues of patients with NSCLC, the positive expressing rate of LRP was 77.5%, and was not related with age, sex, smoking history, histological type, differentiation, distant metastasis, lymphatic metastasis(P>0.05). In the platinum contained chemotherapy regimens, there were not statistical significant difference in RR, DCR, PFS, and OS between the LRP positive expression group and the LRP negative expression group(all P>0.05). The positive expression rate of LRP in female patients(95.5%) was higher than that in male patients(71.6%；P=0.043). The positive expression rate in the patient with distant metastasis(87.3%) was higher than that in the patients without distant metastasis（61.8%）(P=0.005).Conclusion　The positive expression of LRP is related to the diagnosis distant metastasis of NSCLC. The PFS, OS in LRP-positive patients are lower than those in LRP negative patients. These results suggest LRP may be an evaluating indicator of the prognosis of NSCLC.]]> 2018/1/15 8:42:20 LIU Qiong, HUANG Chang-jie, HUANG Wen-feng, et al. 564true ［Abstract］　Objective　To construct the eukaryotic expression vectors of 3′untranslated regions（3′UTR） of CCL11 gene and CCL26 gene.Methods　Target fragments in 3′UTR of CCL11 gene and CCL26 gene were amplified from genomic DNA by Polymerase chain reaction(PCR).The target fragments and pMIR REPORT vectors were double cut by restriction enzyme,and then the fragments were inserted into the pMIR REPORT plasmid to construct recombinant plasmid.The recombinant plasmid of pMIR REPORT-CCL11 3′UTR and pMIR REPORT- CCL26 3′UTR were transformed DH5α competent cells,respectively.Results　The 3′UTR sequence of CCL11 gene and CCL26 gene were successfully cloned into the pMIR REPORT vector,which verified by restrictive enzyme digestion and DNA sequencing.Conclusion　The recombinant plasmid of pMIR REPOR-CCL11 3′UTR and pMIR REPORT- CCL26 3′UTR were successfully constructed,which lay a foundation of the further study of the relation between CCL11 gene,CCL26 gene and microRNAs.]]> 2018/1/15 8:42:20 WEN Zhi-hong, DAI Yan, HE Shuang 563true ［Abstract］　Objective　To investigate the relationship between osteoprotegerin(OPG）and type 2 diabetes mellitus(T2DM) with or without coronary artery disease(CAD).Methods　The healthy individuals(control group), T2DM patients with or without CAD with 30 cases in each group were selected respectively.ELISA was used for determination of OPG, biochemistry method for fasting blood glucose(FPG) and glycosylated hemoglobin（HbA1c）, immunochemical chemiluminescence for fasting insulin(FINS), insulin sensitivity index(ISI) and insulin resistance index(HOMA-IR) were calculated，and the data in all groups were observed.Results　OPG, FPG,HbA1c and HOMA-IR in groups of T2DM with or without coronary artery disease were obviously higher than those in control group（P<0.01）,and ISI was lower than that in control group（P<0.01）.FINS in the group of T2DM with CAD was higher than that in control group（P<0.05）.HbA1c in the group of T2DM was higher than that in the group of T2DM with CAD(P<0.01).Conclusion　The level of serum OPG,blood glucose,insulin and insulin resistance index in groups of T2DM with or without CAD increase obviously. Development of CAD in T2DM patients relate to the increased level of OPG,blood glucose,insulin and insulin resistance. OPG as a risk predictor of cardiovascular disease may be related to the effects of insulin.]]> 2018/1/15 8:42:20 CHEN Hui, LIU Ling，LU Wei-jun，et al. 562true ［Abstract］　Objective　To detect the changes of the levels of porphromonas gingivalis(Pg) and clinical index and to investigate the relationship between the bacterial load and the periodontal status.Methods　Periodontitis patients with type 2 diabetes were randomly divided into experimental group and control group. Clinical index were examined and subgingival plaques were collected before and 6 months after basal periodontal treatment in experimental group; Clinical index were examined and subgingival plaques were collected before and 6 months without any treatment in control group.Results　Sulcus bleeding index(SBI) and attachment loss(AL) were significantly decreased in experimental group after 6 months（P<0.01）, but SBI and AL were no significantly differences before and 6 months in control group（P<0.05）. The numbers of Pg were no significantly differences before and 6 months in both two groups.Conclusion　There were good curative effect of basal periodontal treatment in improving clinical index, but it is difficult to remove periodontal pathogens thoroughly.]]> 2018/1/15 8:42:20 WEI Yi，ZHOU Yan, LIU Hong-yan, et al. 561true ［Abstract］　Objective　To discover the acquired risk factors for pulmonary embolism(PE) in Guangxi of China.Methods　Thirteen general hospitals in Guangxi were included. The risk factors for PE were chosen based on the PE guidelines of European Society of Cardiology and Chinese Medical Association. The patients with definite PE from 2009 to 2011 were recruited for the risk factors analysis, prospectively.Results　Twenty-one(10.4%) of 202 patients with PE suffered from venous thrombosis. The most common risk factors consisted of the advanced age(103 patients, 51.0%), prolonged immobilization(38 patients, 18.8%), recent surgery operation(27 patients, 13.4%), thrombocytosis(26 patients, 12.9%) and chronic obstructive pulmonary disease(22 patients, 10.9%). Except 41 patients(20.3%) with non-risk factor, one risk factor was found in 75 patients(37.1%) and two or more were in 86 patients(42.6%).Conclusion　The patients with advanced age, immobilization, recent operation, thrombocytosis or chronic obstructive pulmonary disease are more likely to develop PE in Guangxi. Proper prophylaxis should be emphasized for these population.]]> 2018/1/15 8:42:20 QIN Zhi-qiang, JIANG Lian-qiang, CHEN Gui-rong, et al. 560true ［Abstract］　Objective　To explore the application effects of endoscopic thyroid surgery.Methods　All the endoscopic thyroid surgeries carried out in Laibin People′s Hospital from June 2008 to December 2012 were retrospectively analyzed.Results　All endoscopic thyroid surgeries in 112 cases were successful, including thyroid tumor removal in 5 cases, partial thyroidectomy with unilateral lobe removal in 6 cases, partial thyroidectomy with bilateral lobes removal in 59 cases, and partial thyroidectomy with removal of unilateral lobe and isthmus of thyroid gland in 42 cases. The patients′ postoperative satisfaction was 100%.Conclusion　Comparing to traditional thyroid surgery, endoscopic thyroid surgery has advantages of mild pain, rapid recovery and satisfactory postoperative cosmetic results.]]> 2018/1/15 8:42:20 HUANG Zi-tuan, LIANG Yong-ming, LI Chang-ming, et al. 559true ［Abstract］　Objective　To explore the clinical effect of application of Milligan-Morgan hemorrhoidectomy combined with indometacin and furazolidone suppository in the treatment of mixed hemorrhoids at stage Ⅲ.Methods　From January 2012 to March 2013,60 patients with mixed hemarhoid at stage Ⅲ were randomly divided into two groups(30 cases in each group). The patients in the control group received routine operation therapy.On the basis of above therapy,the patients in the treatment group were given indometacin and furazolidone suppository,putting one suppository into the rectal cavity at the end of operation,and then one into anus when exchanging the dressing on the first postoperative day.The therapeutic effects were observed in two groups.Results　The therapeutic effets in the treatment group were superior to those in the control group（P<0.05） and the average hospitalization days in the treatment group were shorter than those in the control group（P<0.01）.The severity degrees of compications(pain, anal bulge sense, edema and hemorrhage) in the trentacant group were milder than those in the control group（P<0.05 or P<0.01）.Conclusion　The application of milligan Morgan hemorrhoidectomy combined with indometacin and furazolidone suppository in the treatment of mixed hemorrhoids at stage Ⅲ solves the problem of postoperative analgesia,long recovery time and more complications,and is worthy of clinical use.]]> 2018/1/15 8:42:20 ZHU Fu-qing 558true ［Abstract］　Objective　To evaluate the clinic application value of extracorporeal shock wave(ESW) combined with hyperbaric oxygen(HBO) in the treatment in bone ununion after internal fixation of limbs fracture.Methods　A retrospective analysis was performed of 87 bone ununion patients after internal fixation surgery of limbs fracture(gap less than 10 mm,age less than 60 years).Among them, 46 patients in the trial group(group A) were treated by ESW combined with HBO, 41 patients in the control group(group B) were treated by ESW only. At 1, 3, 6, 9, 12 months after the treatment the X-ray film were fallen and local inspection were performed to observe the treatment condition.Finally,the curative effect were analyed by statistical software.Results　The cure rate of group A was higher than group B, the difference was statistically significant(P<0.05), the fellow-up results at 3, 6 months showed the effective rate of group A was higher than group B, the difference was statistically significant(P<0.05).Conclusion　The ESW combined with HBO has the advantage of easy to operation,less trauma and it is effective for the bone ununion（gap less than 10 mm） patients. The cure rate and early effective rate of ESW combined with HBO is better than ESW only, so it is a good choice for bone ununion patients after internal fixation of limbs fracture.]]> 2017/12/18 8:27:35 LI Hong-yu, YU Shao-lin, LIU Bo-yu, et al. 557true ［Abstract］　Objective　To investigate the clinical outcomes of Quadrant system modified transforaminal lumbar interbody fusion(modified TLIF) for treatment of lumbar degenerative disease.Methods　Eigty-seven patients with lumbar degenerative disease were treated by modified TLIF assisted by Quadrant expandable tube channel minimally invasive system, via posterior multifidus muscle gap approach. Clinical outcomes were assessed by the VAS pain scores and JOA scores. Pre-operative and post-operative conditions and complicaions were monitored.Results　In all of 87 patients, the mean operation time was 189 min, the mean incision length was 4.1 cm, the mean operative blood loss was 260 ml and the post-operative drainage was 105 ml. Two patients complicated with dural tears were cured after treatment. All patients were followed-up for 13.5 months, VAS pain scores and JOA scores of one week post-operation and last visit were significantly lower than that of pre-operation(P<0.05).Conclusion　Applying Quadrant expandable tube channel modified TLIF for treatment of lumbar degenerative disease had the advantages of less damage, bleeding and time for recovery, with acknowledged short-term outcomes. It is a safe and effective minimally invasive operation method.]]> 2017/12/18 8:27:35 LAN Min-dong, WEI Jian-xun, LI Rong-zhu, et al. 556true ［Abstract］　Objective　To study the bacterial pathogen characteristics of children with community-acquired pneumonia(CAP) and antimicrobial susceptibility for clinical pneumonia treatment.Methods　The clinical features, bacterial pathogen characteristics and microbial sensitivity test of 956 cases of hospitalized children with CAP were systematically analyzed.Results　Gram-negative(G^-) bacteria was detected in sputum culture from 653 of 956 cases of children with CAP, accounting for 68.3%; Gram-positive(G⁺) cocci in 303 cases, accounting for 31.7%. Among them, E.coli was detected in 253 cases(26.5%), klebsiella pneumoniae in 166 cases(17.4%), haemophilus influenzae in 78 cases(8.2%), acinetobacter baumannii in 53 cases(5.5%), Pseudomonas aeruginosa in 41 cases(4.3%), Streptococcus pneumoniae in 47 cases(4.9%) and staphylococcus aureus in 41 cases(4.3%). Common original pathogen of children with CAP in the secondary hospital were haemophilus influenzae, moraxelle catarrhalis and Streptococcus pneumoniae. While those in the tertiary hospitals were E. coli, klebsiella pneumoniae, acinetobacter baumannii and coagulase negative staphylococcus aureus. Klebsiella pneumoniae were partly resistant to Tienam and Meiluopeinan, and acinetobacter baumannii were also resistant to Tienam. Streptococcus pneumoniae were generally resistant to penicillin, and sulfamethoxazole, clindamycin and erythromycin and azithromycin. Staphylococcus aureus was completely resistant to penicillin and also highly resistant to sulfamethoxazole, ampicillin, erythromycin, azithromycin and tetracycline.Conclusion　G^- bacteria in children with CAP were not uncommon, while there be likely needed to pay seriously attention to higher antimicrobial resistance to bacteria.]]> 2017/12/18 8:27:35 XIE Qing-ling, ZHEN Hong, TANG Xiao-yan, et al. 555true ［Abstract］　Objective　To study the effect of smoking on male sperm morphology,sperm DNA integrity rate ［DNA fragmentation index(DFI) was used for the evaluating］ and acrosome integrity rate.Methods　A retrospective analysis was performed on 1 500 male patients come to our center for treatment（1 300 smokers and 200 non-smokers）. According to the daily smoking quantity the patients were divided into A1 group(500 cases):>20/day, A2 group(600 cases):10～20/day, A3 group(200 cases):1～9/day； and group B(200 cases):0/day(as the control group).Results　The normal morphology rate of sperm decreased with the increase of daily smoking quantity, the pair comparison showed a significant differences between groups(P<0.05); the rate of sperm DNA integrity decreased with the rise of smoking(DFI will be increase), the pair comparison showed a significant differences between groups(P<0.05); the sperm acrosome integrity rate decreased with the increase of smoking quantity, the pair comparison showed a significant differences between groups(P<0.05).Conclusion　With the increase of daily smoking quantity the rate of normal sperm morphology, sperm DNA integrity rate and sperm acrosome integrity rate are reduced,and is not good for male reproductive health.]]> 2017/12/18 8:27:35 HE Yong-zhi, LI Da-wen, XIAO Xin, et al. 554true ［Abstract］　Objective　To investigate the status and the influential factors of child neglect among children aged 3～6 years in Nanning, and to provide evidence for the intervention strategy.Methods　Survey was performed in the 1 042 children′s parents, who were recruited from 10 kindergartens using stratified cluster sampling technology, by questionnaires.Results　The prevalence rate of children′s neglect was 6.3% and the children′s neglect score was （41.4±6.9） in urban children in Nanning. The prevalence rate and score of children′s neglect were varied with age(P<0.05), rather than gender(P>0.05). The main influencing factors of prevalence rate of children′s neglect were children′s age, mother′s education level, type of family, rank of child in family, with or without genetic disease and parental income reduced or not. The influencing factors of neglect score were mother′ s education, children′s age, with or without genetic disease, type of family, housing area and parental income reduced or not.Conclusion　Prevalence rate of child neglect is low, but the strength of child neglect is relative high among urban children in Nanning. Mother′ s education, children′s age, type of family, with or without genetic disease and parental income reduced or not are closely related to child neglect.]]> 2017/12/18 8:27:35 SU Fa-wo, GANG Yu, XIAO Xin, et al. 553true ［Abstract］　Objective　To investigate drug resistance and resistance phenotypes of Elizabethkingia meningoseptica（EME）.Methods　The drug resistance rates of 30 strains to 22 antibiotics were detected by MIC and disk dilution method. Phenotypes of extended spectrum β-lactamase（ESBLs）, Ampc and metallo β-lactamase（MBL） were detected by disk diffusion and modified three dimension test.Results　The drug resistance rates of 30 strains to β-lactam grop drugs were above 70%, all strains were more susceptible to trimethoprim-sulfa（SXT）, ciprofloxacin, levofloxacin, piperacillin/tazobactam, cefoperazone/sulbactam. The drug resistance rates to vancomycin and rifampi were 23.3% and 16.7% respectively. All the isolates were detected to have ESBLs but no Ampc, 18 strains（60.0%） had MBL.Conclusion　EME was multi-drug resistance, it can produce ESBLs and MBL.While ESBLs was most responsible for the resistance to β-lactam group drugs. EME was more resistance to imipenem and meropenem which were used to cure most of the gram negative bacilli, but more susceptible to vancomycin and rifampin which were used to cure most of the gram positive coccus. SXT, minocycline,β-lactamase inhibitor and quinolones drugs may be the best choice for its infection.]]> 2017/12/18 8:27:35 CHEN Xing-chun, NONG Sheng-zhou, ZHAO Li, et al. 552true ［Abstract］　Objective　To investigate the clinical efficacy of penxiao enema in the treatment of chronic pelvic inflammatory disease(CPID).Methods　One hundred and twenty patients with CPID of dam-heat stasis type were randomly divided into three groups, with 40 cases in each group. Ethanol extraction group was treated by retention enema of penxiao enema(ethanol extraction), water extraction group was treated by retention enema of penxiao enema(water extraction), kangfu anti-inflammatory suppositories group was treated by Kangfu anti-inflammatory suppositories via rectum. Three groups were treated for two courses, the difference in the therapeutic effects before and after among the three groups was compared.Results　The therapeutic effect of penxiao enema was better than that of Kangfu anti-inflammatory suppositories via rectum(P<0.01); ethanol extraction group was superior than the other two groups in therapeutic effects, and the former′s adverse reactions was less, and was well tolerated.Conclusion　The therapeutic effect of penxiao enema (ethanol extraction) in the treatment of patients with CPID of dam-heat stasis type was significant.]]> 2017/12/18 8:27:35 YI Lei, ZHANG Hai-lin, DENG Ping, et al. 551true ［Abstract］　Objective　To investigate the clinical value of selective single blastocyst transfer, and effect of blastocyst developmental potential of the inner cell mass(ICM) and trophectoderm(TE) by non-selective single blastocyst transfer study.Methods　Two hundreds and seventy-six cycles of non-selective single blastocyst transfer in the center were selected and the rate of pregnancy and the early abortion rate of high-quality single blastocyst were compared with non-quality single blastocyst，and the rate of pregnancy and the early abortion rate in different levels of ICM and TE were compared.Results　The clinical pregnancy rate of quality single blastocyst transfer was significantly higher than that of non-quality single blastocyst transfer(P<0.05); the clinical pregnancy rate of ICM for A was significantly higher than that of ICM for C(P<0.05); the clinical pregnancy rate of TE for A was significantly higher than that of the TE for B or C(P<0.05); the early abortion rate between the groups was not significant; Multiple pregnancy rate was 1.16%.Conclusion　Multiple birth rate of single blastocyst transfer is very low; Quality single blastocyst transfer can get a higher clinical pregnancy rate than non-quality single blastocyst transfer; CC can obtain a low pregnancy rate, so in no other case of embryos, CC blastocyst can be transferd after the fully informed consent.]]> 2017/12/18 8:27:35 HE Bing, CHENG Jun-ping, HUANG Li, et al. 550true ［Abstract］　Objective　To explore the impact of a training program for venous thromboembolism on the diagnosis and prognosis of pulmonary embolism(PE) in Guangxi.Methods　Thirteen hospitals in Guangxi were included and the groups consisting of doctors and nurses of respiratory department, radiologists and sonologists from the hospitals were trained intensively in a class in late 2008. The training program included venous thromboembolism epidemiology, clinical manifestation, diagnosis and management. After that, the trainers guided the trainees through internet, telephone or at present. The training effect were evaluated by comparison of PE cases, diagnostic techniques and hospital mortality between 2005～2007 and 2009～2011.Results　Comparing with 2005～2007, PE incidence in respiratory hospitalizations raised significantly from 2009 to 2011［1.03%(604/58 530) vs 0.71%(273/38 581), P<0.001］ and proportion of definite PE cases to all PE also increased significantly［73.01%(441/604) vs 64.84%(177/273), P=0.014］. CT pulmonary angiography was the key diagnostic technique in both two periods(95.69% among 2005～2007 and 92.95% among 2009～2011). The hospital mortality in 2005～2007 and 2009～2011 were 16.12%(44/273) and 8.61%(52/604), respectively(P=0.017).Conclusion　Training program of venous thromboembolism improves the level of diagnosis and prognosis of PE.]]> 2017/12/19 8:06:01 QIN Zhi-qiang, CHEN Gui-rong, CHEN Xian-yuan, et al. 549true ［Abstract］　Objective　To evaluate the change of the cant of the occlusal plane of the patients with classⅠbia-maxillary protrusion before and after orthodontic treatment.Methods　Nineteen patients with classⅠbia-maxillary protrusion were extracted by 4 first premolars and treated with straight wire appliance. Pre-treatment and post-treatment lateral cephalograms of the patients were obtained and evaluated to determine the difference in the cant of the occlusal plane.Results　The cant of anterior occlusal plane（AOP-FH）was (10.50±2.59)° before treatment,(13.18±4.21)° after treatment, and the difference was significant（P<0.05）. The change of the cant of posterior occlusal plane（POP-FH）showed no statistical difference(P>0.05).Conclusion　After treatment, the cant of posterior occlusal plane of the patients with classⅠbia-maxillary protrusion remain stable. But the cant of anterior occlusal plane become larger and this change have something to do with the lingual extrusion and relative elongation of upper teeth.]]> 2017/12/19 8:06:01 FANG Zhi-xin, ZHOU Yan, GUO Yong-ming, et al. 548true ［Abstract］　Objective　To explore the clinical value of ulrtasonic detection technology in infertile men with obstructive azoospermia.Methods　The reproduction organs of 129 infertile men with obstructive azoospermia were detected by ultrasonography, then the abnormal detection rate and its clinical significance were analyzed.Results　There were significant differences in the abnormal detection rate and absence rate among various organs of 258 organs in 129 cases（χ²=393.581，P=0.000）. The abnormal detection rate of the organs in the sequence of decending was ejaculatory duct(72.87%), epididymis(48.45%), seminal vesicle(46.90%), testis(27.52%)and vas deferens(8.91%), while the absence rate was seminal vesicle(24.42%), epididymis(12.02%),vas deferens(11.63%), testis(1.16%), ejaculatory duct(0.00%).Conclusion　Ulrtasonic detection technology can provide an objective basis for judging the obstructive site in infertile men with obstructive azoospermia, and the ultrasonic detection possesses important clinical value for treatment options in male infertility.]]> 2017/12/19 8:06:01 WAN Li-kai, LI Da-wen, WANG Xiao-ping, et al. 547true ［Abstract］　Objective　To evaluate the influence of intraperitoneal hyperthermic perfusion chemotherapy on immune indexes in peripheral blood in postoperative patients with colorectal cancer.Methods　From January 2010 to December 2013,50 patients with DukesB～C colorectal cancer were randomly divided into two groups:the hyperthermic perfusion chemotherapy group and the normal temperature perfusion chemotherapy group.After surgery hyperthermic perfusion chemotherapy group received intraperitoneal hyperthermic distilled water(43～45 ℃) perfusion containing fluorouracil(5-FU)0.5 g/L,mitomycinC(MMC) 8 mg/L oxaliplatin 25 mg/L in 4 000 ml distilled water for 1 hour; the normal temperature perfusion group received intraperitoneal normal temperature distilled water(25～30 ℃) perfusion containing above drugs in 4 000 ml distilled water for 1 hour.The same perfusions were performed at 1 and 2 months after operation in two groups. Before operation,during operation and at 1 and 2 months after operation IL-2 and TNF-α in peripheral blood were measured by ELISA in two groups.Results　Compared with normal temperature perfusion chemotherapy group,the levels of IL-2 and TNF-α in hyperthermic perfusion chemotherapy group were significantly increased,and the difference was statistically significant(P<0.05).Conclusion　Intraperitoneal hyperthermic perfusion chemotherapy may promote Thl-type immune drift, enhance the body′s anti-tumor immune function, and have a certain role in prevention and treatment of the peritoneal recurrence and metastases of colorectal cancer.]]> 2017/12/19 8:06:01 PANG Li-ming, LI De-gang, WU Wei 546true ［Abstract］　Objective　To investigate the relationship between antinuclear antibody(ANA) fluorescence model, specific antibodies spectrum, titer and autoimmune diseases（AID）.Methods　Four hundred and four patients with AID were investigated by using indirect immunofluorescence assay to detect ANA, at the same time, using Europe and Mongolia blotting to detect 15 specific antinuclear antibodies spectrum(ANAS), and a comparative analysis was performed of expression of ANA fluorescence model and the titer and ANA fluorescence model in different diseases.Results　In AID group the positive rate of ANA was 68.81%, titers in 1∶1 000 accounted for 75.90%. ANA fluorescence model mainly showed cytoplasmic type and nuclear homogeneous type, 130 cases and 108 cases respectively. In ANA positive patients, the titer of ANA was higher and fluorescent hybrid model was also increased.Conclusion　The detection rate of ANA was higher in patients with AID. In fluorescent ANA positive patients with high titer the fluorescence model mainly is hybrid model. The simultaneous detection of ANA and ANAS has important clinical significance for diagnosis of autoimmune diseases.]]> 2017/12/19 8:06:01 LI Yan-mei, MO Si-jian, LIANG Tai-ying, et al. 545true ［Abstract］　Objective　To compare the clinical efficacy of transforaminal lumbar interbody fusion via Quadrant minimally invasive system(mini-TLIF) versus traditional open transforaminal lumbar interbody fusion(open-TILF) in the lumbar spine spondylolisthesis.Methods　This was a retrospective review of collected data.Between January 2010 and January 2012，40 patients with low-grade spondylolisthesis were operated in our hospital,20 patients underwent open-TILF and 20 patients underwent mini-TLIF.The blood loss, length of cut,operating time,postoperative bedrid time in the two groups were recorded perioperatively.Pain and functional disability were measured using visual analogue scale(VAS) and oswestry disability index(ODI) before operation,at 2 weeks,2 months,1 year and 2 years after operation.The therapeutic effects were compared between two groups after operations.Results　In aspect of the blood loss,length of cut,postoperative bedrid time,the mini-TLIF group was better(P<0.01),while the open-TILF group was better in the operating time(P<0.05).Postoperative VAS and ODI scores in two groups were declining after operation,each point scores were statistically significant difference(P<0.01) compared with preoperative scores.The postoperative VAS and ODI scores in mini-TLIF group decreased more markedly than those in the open-TILF group,the difference was statistically significant(P<0.01).The scores of VAS and ODI of mini-TLIF group were less than those in open-TILF group at 2 weeks and 2 months after operation(P<0.01).Conclusion　The transforaminal lumbar interbody fusion operation by Quadrant system to treat the patients with lumbar spondylolisthesis is more simple and efficient than open TILF.It has advantages in the blood loss, length of cut, postoperative bedrid time and the short-term postoperative pain.Provided strictly master the operation indications,it is worth to be promoted.]]> 2017/12/19 8:06:01 GU Jin, LIANG Bin, LI Rong-zhu, et al. 544true ［Abstract］　Objective　To prepare diagnostic kit for HIV-Ⅰ/Ⅱ antibodies with mixed polypeptide antigen including HIV-Ⅰgp41, gp120, p24 and HIV-Ⅱ gp36.Methods　The appropriate concentration of coated antigen and HRP-Ag were selected by checkerboard titration, and the sensitivity and specificity of the kit were conducted.Results　The appropriate concentration of coated antigen was 5 μg/ml, and the appropriate concentration of HRP-Ag was 1∶160. After optimizing the detection system, the CUT-OFF value, sensitivity and specificity of the kit were 0.207, 97.2% and 98.6%, respectively.Conclusion　It is feasible that detect HIV infection via polypeptide antigen coated in polystyrene plate with high sensitivity and specificity.]]> 2017/12/25 16:01:51 WU Jun-rong, LI Shan, SHEN Wei-dong, et al. 543true ［Abstract］　Objective　To explore the relationship between serum Ghrelin level and insulin resistance in infertility patients with PCOS.Methods　Ninety-eight cases of PCOS （PCOS group）and seventy-two cases of non PCOS(non PCOS group) for first time visiting our hospital due to infertility from January 2010 to December 2012 were selected.The BMI, serum Ghrelin level, and the related anthropometric indexes were detected.Results　The serum Ghrelin levels in PCOS patients were lower than non PCOS patients［(442.56±221.71)pg/ml vs (697.41±157.84)pg/ml, P<0.05］. The HOMA-IR was higher in PCOS group compared to non PCOS group［（3.84±1.23） vs (2.14±0.77), P<0.05］. Pearson correlation analysis showed that serum Ghrelin level was negatively correlated with HOMA-IR in PCOS patients(r=-0.48, P<0.01).Conclusion　The serum Ghrelin level in infertility patients with PCOS is evidently lower than that in non PCOS infertility patients. Ghrelin level was negatively correlated with HOMA-IR in PCOS patients. Thus indicated that Ghrelin may promote the development of IR in infertility patients with PCOS.]]> 2017/12/25 16:01:51 HUANG Hong-yi, HE Bing, MO Yun, et al. 542true ［Abstract］　Objective　To assess interfractional and intrafractional errors and to estimate appropriate margins for planning target volume(PTV) by using daily cone-beam computed tomography(CBCT) guidance in nasopharyngeal carcinoma(NPC) treaded with intensity -modulated readiation therapy(IMRT).Methods　Daily pretreatment and posttreatment CBCT scans were acquired separately after initial patient setup and after the completion of each treatment fraction in 10 patients treated with IMRT. Online corrections were made before treatment if any translational setup error was found. Interfractional and intrafractional errors were recorded in the right-left(RL), superior-inferior(SI), and anterior-posterior(AP) directions.Results　For the translational shifts, the interfractional errors >2 mm occurred in 21.7% of measurements in the RL direction, 12.7% in the SI direction, and 34.1% in the AP direction, respectively. Online correction resulted in 100% of residual errors ≤2 mm in the RL and SI directions, and 95.5% of residual errors ≤2 mm in the AP direction. No residual errors >3 mm occurred in the three directions. For the rotational shifts, significant reduction was found in the magnitudes of residual errors compared with those of interfractional errors. A margin of 4.9 mm, 4.0 mm, and 6.3 mm was required in the RL, SI, and AP directions, respectively, when daily CBCT scans were not performed. With daily CBCT, the margins were reduced to 1.2 mm in all directions.Conclusion　Daily CBCT guidance is an effective modality to improve the accuracy of IMRT for NPC. The online correction could result in a 70%～81% reduction in margin size.]]> 2017/12/25 16:01:51 LU He-ming, FENG Guo-sheng, CHEN Jia-xin, et al. 541true ［Abstract］　Objective　To compare the histological diagnosis results of the cervical atypical squamous cells of undetermined significance(ASCUS) in liquid based cytology in Guangxi district, looking for a reasonable management for the ASCUS patients.Methods　The clinicopathological data of 237 cases of ASCUS with histological diagnosis were studied.Results　Clinically, the age of 237 patients were ranged from 19 to 67 years old, the median age was 41 years, 48 cases of them were postmenopausal women, 94%(223 cases) of the patients were asymptoms. Gynecological examination found that 46%(108 cases) of them had smooth cervix, 44%(105 cases) had cervical erosion, and 10%(23 cases) had cervical hypertrophy. The histological diagnosis showed that cervical chronic inflammation with squamous epithelial hyperplasia and cervical chronic inflammation with glandular epithelial metaplasia was found in 121cases(51.05%), cervical condyloma in 31 cases(13.08%), CINⅠ in 54 cases(22.78%), CINⅡ and Ⅲ in 28 cases(11.80%), and carcinoma in 3 cases(1.30%).Conclusion　Most of the ASCUS patients in Guangxi district are asymptoms. Colposcopy examination may be offered in ASCUS patients, and biopsy should be done at the same time when high-grade CIN or tumor are suspected, in order to find high-grade cervical precancerous lesions and cancer timely.]]> 2017/12/25 16:01:51 ZHOU Xiang-zhen, LU Jun-ying, SHEN Xiao-xiong, et al. 540true ［Abstract］　Objective　To analyze the occurring characteristic and preventive measures of nosocomial acquired pneumonia of acinetobacter baumanii(Ab) in neurology intensive care unit(NICU).Methods　The data of nosocomial infection for the medical records in NICU were analyzed by prospective monitoring studies.Results　Nosocomial acquired pneumonia of Ab occurred in 9.04 % patients admitted to the NICU. The most patients with nosocomial acquired pneumonia of Ab had chronic diseases and underwent many kinds of invading operation. The rate of nosocomial acquired pneumonia of Ab in stroke patients were higher. In the vitro drug susceptibility test，only the sensitive rates of carbapenems and cefoperazone sulbactam were higher, and Ab were multi-resistant to cephalosporins, aminoglycosides and fluoroquinolone antibiotics.Conclusion　The drug resistance of Ab is severe and multi-resistant to antibiotics, so it is necessary to obey aseptic operation rule strictly, use the antibiotics rationally and strengthen monitoring of drug resistance of Ab.]]> 2017/12/25 16:01:51 LI Yan-hua, LI Lv-li, FAN Bing-lin, et al. 539true ［Abstract］　Objective　To investigate the clinical effect of treating preschool-aged children with cerebral palsy by occupational therapy in groups.Methods　Twenty children with spastic diplegia were randomly divided into control group(n=10) and observation group(n=10). Occupational therapy of the control group was conducted in a one-to-one training way while the observation group was conducted in groups of 2 children once a day, each time lasting for 30 minutes, 5 days each week for 3 months.Results　After the therapy, the Fine Motor Function(FMF) and Activities of Daily Living(ADL) in both groups significantly improved(P<0.01) and there was no significant difference between the results of the control group and the observation group（P>0.05）.Conclusion　Both occupational therapy in the ways of groups and traditional one-to-one can substantially improve the FMF and ADL of children with cerebral palsy, and there is no significant difference in the treatment effect.]]> 2017/12/25 16:01:51 TAN Wei-wei, CHEN Guo-zhi, ZHANG Ming-wu, et al. 538true ［Abstract］　Objective　To study the classification criteria of biliary calculi with infection of biliary tract and choice of the operation mode and operation timing for the elderly patients.Methods　106 cases of elderly patients who were suffered from biliary calculi and biliary tract infections and treated in the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2010 to December 2012, were divided into 3 groups according to our prvious classification criteria:mild acute biliary tract infection in 71 cases(group A), moderately acute severe biliary tract infection in 24 cases(group B), acute severe biliary tract infection in 11 cases(group C). There were no statistical differences among the three groups in gender, age, types of stones and the complicated diseases. The operation mode was biliary drainage and biliary lithotomy. Biliary drainage included ENBD, PTCD and common bile duct T-tube. Biliary lithotomy included common bile duct lithotomy bay Laparoscopic or open operation and EST.Results　There were significant differences among the three groups in the length of ICU stay, postoperative hospitalization days and total hospitalization days(P<0.01),and in the residual stone rate(P<0.05). No dead cases were found in the three groups after the operation. The amount of intraoperative bleeding and postoperative complication rate were not significantly different among the three groups(P>0.05).Conclusion　Our classification criteria of biliary calculi in elderly patients with biliary tract infection are helpful for the choice of operation mode and operation timing for the patients and can improve the elderly patients′ clinical efficacy of biliary calculi with biliary tract infections.]]> 2017/12/4 8:38:51 SU Zhi, LIU Tian-qi 537true ［Abstract］　Objective　To explore the appropriate culture conditions of umbilical cord blood mesenchymal stem cells.Methods　The sterile cord blood from normal full-term newborns was randomly divided into three groups: the high sugar DMEM group, the low sugar DMEM group and the umbilical cord blood mesenchymal stem cells between groups. Extractions of umbilical cord blood mononuclear cells were inoculated in umbilical cord blood mesenchymal stem cells in the culture media by the density of 1×10⁵, 1×10⁴, and 1×10⁶ cells/ml respectively. The three kinds of media and different inoculation density culture adherent cell growth situations were observed and analysis of cell surface antigen was performed using flow cytometry technique.Results　Ectomesenchymal stem cells of sugar DMEM group did not grow on the sidewall, while the growth of the spindle formation fiber sample cells was found on the sidewall in low sugar DMEM group and umbilical cord blood mesenchymal stem cell(P<0.05). Of different inoculation densities, cells grew best at the density of 1×10⁵, followed by 1×10⁶. Cells failed to grow at the density of 1×10⁴.Conclusion　Umbilical cord blood mononuclear cells in T25 culture bottle grow best at the inoculation density of 1×10⁵/ml, which is the most suitable culture medium for umbilical cord blood mesenchymal stem cell.]]> 2017/12/4 8:38:51 XUE Xing-ying, KE Ru-xiang, QIN Jin-ding, et al. 536true ［Abstract］　Objective　To investigate the value of retraction phenomenon at three-dimensional ultrasonography(3D-US) in diagnosis and prognosis evaluation in breast lesions.Methods　165 patients with breast lesions who underwent 3D-US examination were included. The presence of the retraction phenomenon in the coronal plane was evaluated. Pathologic prognostic factors, including tumor size, histologic grade, lymph node status, estrogen receptor(ER), progesterone receptor(PR) status, human epidermal growth factor receptor 2(Cerb-B2) and Ki-67 expression were determined. The correlation of the retraction phenomenon and prognostic factors was analyzed.Results　The sensitivity and specificity of retraction phenomenon in the diagnosis of breast cancers were 67.7% and 93.1% respectively. The detection rates of the retraction phenomenon in the invasive cancer and the non-invasive cancer were 80.3% and 11.8%(P<0.001). The retraction phenomenon was correlated to the tumor size, histologic grade, estrogen and progesterone receptor status. Compared with the breast cancer without the retraction phenomenon, the tumor size with the retraction phenomenon was more likely to show a smaller diameter less than 2 cm(P<0.05), a lower histologic grade with grade Ⅱ(P<0.05), and a higher positive rate of ER and PR(P<0.05). The lymph node status and the expression of the CerB-2 and Ki-67 in the two groups had no significant difference(P>0.05).Conclusion　The retraction phenomenon of three-dimensional US may be useful in the diagnosis and prognosis evaluation in breast lesions.]]> 2017/12/4 8:38:51 ZHANG Bing, WANG Xiao-yan, NONG Mei-fen, et al. 535true ［Abstract］　Objective　To investigate the effect of fast track surgery(FTS) on the patients with mixed hemorrhoids in the perioperative period.Methods　78 patients with mixed hemorrhoids were randomly divided into two groups: the observation group(n=40) and the control group(n=38). The control group was treated with conventional methods, while the observation group was treated with fast track surgery in the perioperative period. Both groups were compared in terms of operation and postoperative rehabilitation.Results　Compared with the control group, the FTS group had longer operative time（t=2.189, P<0.05）. The FTS group was better than the control group in less blood loss（t=2.974, P<0.05）, fewer postoperative complications（P<0.05）. There were significant differences in wound healing time, duration of hospitalization, hospital costs（P<0.05） and clinical effect（χ²=2.596, P<0.05）, and better outcome was seen in the observation group.Conclusion　Fast track surgery in perioperative period of mixed heorrhoids can reduce the complications, being helpful for the patients postoperative recovery.]]> 2017/12/4 8:38:51 LIU Chi-wang 534true ［Abstract］　Objective　To investigate the influence of experimental low-gold Ag-Pd alloy and two other dental casting alloys on the color of metal-ceramic restoration.Methods　10 disc samples were casted by each metal. The samples were covered by 0.1 mm OP and 1.1 mm dentin porcelain. The color of each sample was tested and the differences of three dental casting alloys were compared.Results　There were no significant differences in L, C, H values of metal-ceramic restoration with the same thickness of OP and dentin porcelain by 3 different dental casting alloys.Conclusion　There are no significant differences in lightness, chroma and hue of metal-ceramic restorations by 3 different dental casting alloys; Pd-based dental casting alloy cannot significantly influence the color of metal-ceramic restoration.]]> 2017/12/4 8:38:51 TIAN Bao, PAN Xiao-bo, PENG Li-hui, et al. 533true ［Abstract］　Objective　To investigate the clinical application value of three-dimensionally(3D)- mapped puncture biopsy.Methods　A total of 104 patients were recruited as the study subjects and were randomly divided into two groups. MSCT 3D-positioning and 3D-mapped puncture device were used in the study group, while CT cross-sectional image positioning plus conventional puncture needle was used in the control group. The success rate at one time, repeatedly inserting needle rate, diagnostic accuracy, complication rate and puncture accuracy grading were compared between the two groups.Results　The success rate at one time was significantly higher, while the rate of repeatedly inserting needle and complication rate were significantly lower in the study group than those in the control group（P<0.05）. In addition, puncture accuracy grading in the study group was also increased significantly than that in the control group（P<0.01）. However, there was no significant difference in the diagnostic accuracy between the two groups（P>0.05）.Conclusion　Compared with the conventional puncture, 3D-mapped puncture has higher one-time success rate and puncture accuracy grading and lower complication rate.]]> 2017/12/4 8:38:51 ZHANG Kong-yuan 532true ［Abstract］　Objective　To study the prevalence and related risk factors of hypertension among rural population in Guangxi Hepu County and to provide a basis to carry out the hypertension and other chronic disease prevention and control work in rural areas.Methods　 A face-to-face questionnaire survey and physical examination were performed in all the residents aged over 20 years using multi-stage random cluster sampling method in 18 villages from 6 towns in Hepu County.Results　The hypertension prevalence rate of rural residents of Hepu county was 16.05%, of which 16.26% was men, and 15.83% was women(P>0.05). The prevalence rate of hypertension increased with aging(P<0.01). The prevalence rates of hypertension in residents of different villages and towns were different(P<0.01). Logistic regression analysis showed that the hypertension risk factors were age(OR=1.054,95%CI=1.049～1.059), occupation (OR=1.702～9.491, 95%CI= 1.280～14.928), overweight or obese(OR=3.230, 95%CI=2.308～4.521), family history of hypertension(OR=3.790,95%CI=2.554～5.623), meat diet (OR=2.646, 95%CI=1.762～3.974), smoking(OR=2.539, 95%CI= 2.183～2.952), drinking (OR=1.549, 95%CI=1.273～1.885). Senior high school education or above is a protective factor of hypertension(OR=0.669, 95%CI=0.486～0.921).Conclusion　Hypertension prevalence rate of rural residents in Hepu County is on a high level, whose risk factors are overweight and obesity, smoking, drinking alcohol, meat diet. Prevention of chronic diseases, health education and healthy lifestyles should be strengthened to reduce the incidence of hypertension.]]> 2017/12/4 8:38:51 HUANG Zhi-wu, CHEN Yi, XU Jia-zhi, et al. 531true ［Abstract］　Objective　To evaluate the efficacy of applying optical coherence tomography (OCT) in retinal detachment surgery.Methods　The surgery was performed by using scleral buckling±cerclage on 50 eyes of 50 patients with macular-off rhegmatogenous retinal detachment. The submacular fluid height and foveal retinal thickness were measured by OCT examination before surgery and at 1 week, 1, 3, 6 and 12 months after the surgery.Results　The average submacular fluid height decreased from （735.88±532.86）μm to （150.54±188.43）μm postoperatively(t=7.3231, P<0.05) with a follow-up ranged from 13 to 21(16.26±2.59)months. The averages of foveal retinal thickness were （132.12±56.89）μm and （109.18±40.93）μm respectively, before and 1 w after the operation(t=2.3142, P<0.05). Retinal reattachment was found in 47 cases(94.0%) 1 w after the surgery under ophthalmoscope examination and the submacular fluid was totally absorbed in 15 cases(30.0%). As a result, 33 cases(66.0%) got rid of blindness(the best-corrected visual acuity ≥0.05). OCT showed that no foveal fluid was found in 47 cases(94.0%) 12 months after the operation and 48 cases(96.0%) got rid of blindness.Conclusion　OCT can quantitatively measure the submacular fluid height of central macular, objectively evaluate the effects of retinal detachment surgery and provide the evidences for the postoperative treatment.]]> 2017/12/4 8:40:13 ZHAO Xin, LI Min, LV Ming-liang, et al. 530true ［Abstract］　Objective　To explore the effects of methylprednisolone on the levels of TNF-α and IL-17 in the central nervous system of mice with experimental autoimmune encephalomyelitis(EAE).Methods　30 female C57BL/6J mice were randomly divided into 3 groups: methylprednisolone-treated group, EAE group and normal group. The EAE model was induced by immunizing mice with myelin oligodendrocyte glycoprotein peptides(MOG35-55). The brain tissues were collected and their expressions of TNF-α and IL-17 were detected using Western Blot.Results　The neurological deficits of the methylprednisolone-treated mice were lighter than those of the EAE mice(P<0.05), while the methylprednisolone-treated mice displayed the lower expressions of TNF-α and IL-17 than the EAE mice did(P<0.05).Conclusion　Methylprednisolone can protect against EAE in mice by the inhibition of TNF-α and IL-17.]]> 2017/12/4 8:40:13 LI Xiao-feng, LI Lv-li, WEI Jun-jie, et al. 529true ［Abstract］　Objective　To explore the therapeutic effects of super-selective bilateral uterine arterial chemoembolization therapy and radiotherapy for advanced uterine cervical cancer.Methods　41 patients with advanced uterine cervical cancer received super-selective bilateral uterine arterial chemoembolization therapy. The effective rate, clinical beneficial rate and diameter changes of tumor before and after the treatment were observed.Results　The effective rate was 46.3% and the clinical beneficial rate was 85.4%. The tumor diameter after the treatment was smaller than that before the treatment.Conclusion　Super-selective bilateral uterine arterial chemoembolization therapy is an effective treatment for the advanced uterine cervical cancer with significant short-term effects.]]> 2017/12/4 8:40:13 YU Lei, CHEN Yan-hao, LIU Zhi-yong, et al. 528true ［Abstract］　Objective　To observe the relationship between fascia perforating vessels with muscle gap and neural interface in the anterior-lateral approach of the hip join.Methods　33 hips of dead bodies with formaldehyde solution embalmed, and without limb deformity and operation history were anatomised. The fascia vessel near point in the anterior superior iliac spine to the lateral trochanter at the most convex line was explored, and the relationships between the vessels with muscle gap and neural interface were recorded. The numbers of fascia perforating vessels and the position of the muscle gap in 87 hip operations were observed in our department from June 2011 to June 2013.Results　The fascia vascular had constant position in the muscle gap of the gluteus medius muscle and the tensor fascia was found in 30 cases(about 91%) of the specimens.The perforating vessels were found and they came from the deep surface of muscle celearance. The perforating vessels were found in 79 cases(90.8%) who were performed the operation and the peforating vessels came from the deep surface of muscle celearance. The shortest distance from the superior gluteal nerve entry point to fascia latal perforator was (5.62±1.18)cm. There were correlations between the safe range with femur length, the iliac crest and the rotor outside the convex distance(r=0.84,0.61).Conclusion　There is a constant relationship between the postion of the fascial lata perforator vessel with the muscle gap of the gluteus medius muscle and the tensor fascia. The fascial perforating vessels can provide accurate and safe positioning for the OCM approach of the hip join.]]> 2017/12/4 8:40:13 HUANG Yu, SUN Xiao-tong, YIN Dong, et al. 527true ［Abstract］　Objective　To investigate the postoperative effects on the micro-ecology of artificial vagina after smearing hormonal drugs on the patients undergoing the laparoscopic peritoneal vaginoplasty.Methods　80 patients with congenital absence of the vagina were selected and divided into the observation group and the control group. All patients underwent laparoscopic peritoneal vaginoplasty. The patients in the observation group were treated with estradiol benzoate based on the above treatment. The secretions on the artificial vaginal wall were extracted and the micro-ecology of vagina was detected when the filling yarn in the vagina was taken out on the first day, the first week, the second week, the third week and the three and six months(labeled T0～T5) after discharge. Other 40 healthy examined people were selected as the control group who were performed the same detection on the first examination day and at the end of the first week, the second week, the third week, the third month and the sixth month.Results　The artificial vagina pH in the observation group during the period of T0～T1 was close to that in the operation control group but higher than that in the control group(P<0.05). The artificial vagina pH in the observation group during the period of T2～T4 was significantly lower than that in the operation control group(P<0.05), but was significantly higher than that in the control group(P<0.05). The artificial vagina pH in the observation group during the period of T5 was close to that in the control group. The normal rate of micro-ecology of the artificial vagina in the observation group during the period of T0～T3 was close to that in the operation control group but significantly lower than that in the control group(P<0.05). The indexes above in the observation group during the period of T4～T5 were basically normal. The vaginal dominant bacteria in the control group during the period of T0～T3 and in the observation group during the period of T0～T1 were gram positive small coli. The vaginal dominant bacteria in the two groups during other time slots and in the control group were gram positive bacteria.Conclusion　Application of hormonal drugs can improve the micro-ecology of artificial vagina after the peritoneal vaginoplasty.]]> 2017/12/4 8:40:13 YU Hong-biao, QIN Cheng-lu, LUO Guang-nan, et al. 526true ［Abstract］　Objective　To study the relationship between the changes of persistent submacular fluid(SMF) and final vision outcomes in patients with macula-involving retinal detachment(RD) in different diopters after scleral buckling(SB) by using optical coherence tomography(OCT).Methods　50 eyes of 50 patients with macular-off RD were retrospectively analyzed, including 25 eyes in high myopia(HM) group and the other 25 eyes in emmetropes group. The best-corrected visual acuity(BCVA) measurement and OCT scan through the fovea were taken in all patients before and 1 week, 12 months after the surgery. Statistical analysis of the data was performed.Results　There was correlation between HM and persistent SMF after SB. The persistent SMF sustained longer in the HM group (5.96±2.25)months than did those in the emmetropes group (3.75±2.21)months (P<0.05). There was no significant difference in the final visual acuity between the two groups.Conclusion　HM seems to be related to persistent SMF after SB. HM may prolong the absorption time of SMF but not change the visual outcomes in the successful RD repair.]]> 2017/12/4 16:23:24 SHEN Chao-lan, LI Min, LV Ming-liang, et al. 525true ［Abstract］　Objective　To explore the curative effect of plasma exchange combined with CRRT and hemoperfusion in the rescue of acute toadstool poisoning.Methods　Based on the conventional and comprehensive treatment, 6 cases of acute toadstool poisoned patients were treated with plasma exchange, CRRT, and hemoperfusion.Results　Two cases died and 4 cases survived in total 6 cases.Conclusion　Plasma exchange plus CRRT and hemoperfusion is effective in rescuing acute toadstool poisoning.]]> 2017/12/4 16:23:24 XIE Ying, ZHANG Yong-feng, LIN Xin, et al. 524true ［Abstract］　Objective　To explore the short and medium term efficacy and safety of symptomatic intracranial arterial stenosis stenting.Methods　64 patients with symptomatic intracranial arterial stenosis were treated with medication(medication group, n=38) or intracranial arterial stenting(stent group, n=26). NIHSS(US National Stroke Scale) scores and ADL(activities of daily living) scores were compared between the two groups before and after the treatment. The rates of clinical events(including cerebral hemorrhage, cerebral infarction and death) were also assessed and compared between the two groups with a follow-up of three months after the treatment.Results　NIHSS scores and ADL scores were significantly higher in the stent group than those in the medication group(P<0.05). There is no significant difference in the rates of clinical events between the two groups(P>0.05).Conclusion　Symptomatic intracranial arterial stenosis stenting is more safe and effective than medication for the treatment of symptomatic intracranial arterys tenting.]]> 2017/12/4 16:23:24 CHEN Yuan, ZHONG Wei-zhang, LI Lv-li, et al. 523true ［Abstract］　Objective　To explore the value of small incision inguinal hernia mesh repair.Methods　One hundred and seventy-six cases who underwent inguinal herniorrhaphy from January 2012 to January 2014 were included in this prospective controlled study, with 58 cases in small incision inguinal hernia mesh repair(SIIHMR) group, 65 cases in mesh plug hernioplasty(MPH) group and 53 cases in total extraperitoneal（TEP） group. The effectivness, complications and cost of varied approachs of inguinal hernia repair were analyzed and compared among the three groups.Results　The incision length, recurrence,operating time, cost and recovery time were significantly different among the three groups（P<0.05）. There were a lower recurrence and an average length of (3.7±0.8)cm in the SIIHMR group. The longest incision and shortest operating time occurred in the MPH group, and the longest operating time and the highest cost. The shortest incision and the fastest recovery occurred in the TEP group. The complication rates were not significantly different among the three groups（P>0.05）.Conclusion　The three kinds of hernioplasty have their own characteristics, SIIHMR is suitable for the patients who have inguinal hernia with weak transverse fascia or high recurrence risk, especially elderly patients.]]> 2017/12/4 16:23:24 YU Jun-ying, HUANG Shun-rong, FENG Ze-rong, et al. 522true ［Abstract］　Objective　To investigate the efficacy and safety of half-dose reteplase as thrombolytics on more than 75-year-old patients with ST-segment elevation myocardial infarction(STEMI).Methods　65 patients more than 75-year-old with STEMI were randomly divided into the observation group(n=32) and the control group(n=33). Both groups were treated with the primary medication, and the observation group received additional half-dose reteplase. The coronary artery recanalization rate and the occurrence of major adverse cardiovascular events(MACE), bleeding complications and thrombocytopenia were compared between the two groups.Results　The coronary artery recanalization rate in the observation group was significantly higher than that in the control group(68.8% vs 15.2%,P<0.01). The death rate in the observation group was significantly lower than that in the control group(3.1% vs 24.2%,P<0.05). There were no significant differences in the incidences of heart failure, cardiogenic shock and ischemia between the two groups(P>0.05 ). The incidence of mild bleeding complication in the observation group was higher than that in the control group(28.1% vs 6.1%,P<0.05). There were no moderate to severe bleeding complications in the two groups.Conclusion　Half dose of reteplase as thrombolytics is effective and safe for more than 75-year-old patients with STEMI.]]> 2017/12/4 16:23:24 CHEN Heng, GAN Jian-ting, ZHANG Hua-guang, et al. 521true ［Abstract］　Objective　To investigate the correlations between the parameters of prostate volume and the urine dynamics test results in patients with benign prostatic hyperplasia(BPH) using transrectal ultrasound（TRUS）, and to evaluate the value of these parameters on the diagnosis of bladder outlet obstruction.Methods　The clinical data were retrospectively analyzed in 57 patients with BPH. The left/right diameter R1, the up/down diameter R2, the front/back diameter R3 and prostate transition zone volume(TZV) of the prostate were measured by rectum prostatic color ultrasound. The test of parallel urine flow dynamic was performed and the AG value was calculated. According to the values of AG, the patients were divided into group A(AG>40),group B(20≤AG≤40), and group C（AG<20）. Pearson correlation analysis and single factor variance analysis were used to study the correlations between the parameters of prostate volume and AG values.Results　The correlation coefficient between R2 and AG was 0.822, P<0.05; The correlation coefficient between TZV and AG was 0.831, P<0.05. The degree of bladder outlet obstruction was positively correlated with R2 and TZV, but had no correlation with R1 and R3; According to AG values, comparisons of F values among the three groups in R2 and TZV were 33.766 and 33.048 respectively, P<0.05. There were no significant differences in R1 and R3 among the three groups.Conclusion　There is a positive correlation between prostate up/down diameter R2, prostate transition zone volume TZV and bladder outlet obstruction degree, which can be used as diagnosis indicator for urinary tract obstruction caused by prostatic hyperplasia.]]> 2017/12/4 16:23:24 JIANG Yu-feng, SHI Yan-hua, LI Jing, et al. 520true ［Abstract］　Objective　To detect the changes of the carotid artery intima media thickness(IMT), renal function, cardiac structure and function in elderly patients with mild-to-moderate hypertension after antihypertensive treatment of amlodipine.Methods　Forty elderly patients with mild to moderate primary hypertension were selected, and their parameters of IMT, renal function, cardiac structure and function were recorded with the follow-up of six to twelve months after the treatment of amlodipine.Results　After treating with amlodipine for six months, the systolic blood pressure was not significantly decreased(P>0.05), while the diastolic blood pressure was significantly decreased(P<0.01); After one year′s treatment, the systolic blood pressure and diastolic blood pressure were both significanthy decreased(P<0.01). The 24 h ambulatory blood pressure significantly decreased after six months and one year′s treatment (P<0.01). After six months to one year′s treatment, IMT was significantly decreased(P<0.05); Urinary β₂ microglobulin(β₂M), albumin(ALB) and plasma creatinine were significantly decreased(P<0.01); The left ventricular end-diastolic dimension(LVEDd) and the E/A ratio were significantly increased, while the maximum diastolic mitral A-wave velocity measurement(PAV) was significantly decreased(P<0.01); The left ventricular mass index(LVMI), interventricular septum thickness(IVST), left ventricular posterior wall thickness(PWT), maximum diastolic mitral E-wave velocity measurement(PEV), and left ventricular ejection fraction(LVEF) were not significantly changed.Conclusion　Antihypertensive therapy of amlodipine improves carotid IMT, renal function and heart diastolic function.]]> 2017/12/6 8:06:37 WU Yin-xiong, WANG Xiao-yan, ZHANG Qin, et al. 519true ［Abstract］　Objective　To explore the relationship between HbA1c and the occurrence, development and the risk degree of acute coronary syndrome(ACS).Methods　526 patients with ACS were collected as ACS group and 82 healthy people were taken as the control group. HbA1c levels were compared in different types of ACS, severities of coronary artery stenosis and the risk levels to explore the correlation between HbA1c and the occurrence, development, and the risk degree of ACS.Results　HbA1c levels in both STEMI and NSTEMI patients were significantly greater in the ACS group than those in the control group（P<0.05）. HbA1c levels were gradually increased with both the constantly worsened conditions and the rising risk degrees of coronary heart diseases（P<0.05）.Conclusion　HbA1c levels show a trend of increasing with the aggravation of coronary artery diseases. Serum HbA1c level is closely connected with the risk degree of coronary heart diseases, therefore, is a factor of the aggravation of the risk degree of ACS.]]> 2017/12/6 8:06:37 ZHANG Jing-jing, XU Ge 518true ［Abstract］　Objective　To study the effect of low dose glutamin on liver function and endotoxemia in severe chronic hepatitis B patients.Methods　Forty patients with severe chronic hepatitis B were randomly divided into the control group and the experiment group. The control group received the conventional treatment and the experiment group received addiontional glutamine treatment（5 g tid）. The period of the treatment is two weeks. ALT, AST, TBIL, PT, A, CHE and endotoxin(ET) were detected before and after treatment in the two groups respectively.Results　The liver function and serum ET were both improved significantly after two weeks′ treatment, compared with those before treatment in the two groups(P<0.01). Both of the two parameters had no significant difference between the two groups after the treatment（P>0.05）.Conclusion　Adding low dose glutamine(5 g tid) to the conventional treatment can not ameliorate the endotoxemia and liver function in patients with severe chronic hepatitis B.]]> 2017/12/6 8:06:37 YANG Jian-rong, SONG Huai-yu, JIANG Chun-hua 517true ［Abstract］　Objective　To study the effectiveness of using Zhuangyi-Lianhuazhen-Beilangbaguan for treating allergic rhinitis(AR).Methods　Sixty patients with AR in our hospital were randomly divided into two groups(Lianhuazhen group and Fluticasone group). The Lianhuazhen group had a acupuncture using Lianhuazhen and Fluticasone group used Fluticasone nasal spray with a course of treatment of 10 days, and two courses in together. Another course of treatment was resumed after drug withdrawl for one week. The efficacy and S-IgE were compared between the two groups.Results　The efficacy of Lianhuazhen group was more notable than that of Fluticasone group. After the treatment, the expression levels of S-IgE in the two groups were significantly lower than those in the two groups before the treatment (P<0.05) and there were significant differences between the two groups(P<0.05); No adverse reaction was found in the patients of the two groups.Conclusion　The therapy of Zhuangyi-Lianhuazhen-Beilangbaguan is effective and the therapeutic mechanism of inhibiting S-IgE expression may be involved in.]]> 2017/12/6 8:06:37 YANG Pei-pei, FENG Wei-yun, HUANG Gui-hua, et al. 516true ［Abstract］　Objective　To investigate the relationship between ocular ischemic syndrome and carotid artery stenosis.Methods　The carotid atherosclerosis plaque and carotid artery stenosis(CAS) were evaluated using carotid artery color doppler flow imaging(CDFI) and computerized tomography angiography(CTA) in 140 patients with ocular ischemic syndrome(OIS group) and in 140 healthy people(control group). The risk factors of atherosclerosis were analyzed.Results　The number of carotid plaques and CAS in the OIS group was significantly higher than that in the control group(128 vs 40,P<0.05 and 77 vs 24,P<0.05).Conclusion　CDFI is an effective meansure for assessing vascular status.Appropriate intervention for risk factors of atherosclerosis may help in improving the prognosis.]]> 2017/12/6 8:06:37 WEI Chun-ying, ZHANG Yi, HAN Min, et al. 515true ［Abstract］　Objective　To investigate the clinical value of trauma predictor to aim at esophageal varices in alcoholic cirrhosis patients.Methods　One hundred and two alcoholic cirrhosis patients were collected in our hospital from June 2013 to January 2015. These patients were divided into several groups based on the degrees of esophageal varices and other 22 healthy people were chosen as the control group. The changes of four coagulation tests, D-dimer, serum cholinesterase(CHE), gamma glutamyl transpeptidase(γ-GGT), serum albumin(ALB) and the internal diameter of portal vein(PV) were compaired among the different groups.Results　AS the degrees of esophageal varices increased in alcoholic cirrhosis patients, the levels of D-dimer, γ-GGT, PT, TT, APTT and PV increased significantly, and the levels of FIB, CHE and ALB decreased significantly.Conclusion　Early detection of D-dimer has an important clinical value for predicting the degrees of esophageal varices in alcoholic cirrhosis patients.]]> 2017/12/6 8:06:37 HE Can-li, LI Jie-zhuang 514true ［Abstract］　Objective　To analyze the clinical efficacy and safety of linezolid in the treatment of severe sepsis in intensive care unit.Methods　Twenty-seven patients with severe sepsis were treated with linezolid 0.6 gram twice per day and the blood routine examination, liver and renal function were tested before and 72 h after the medication to analyze the efficiency and safety of the treatment.Results　The clinical efficacy rate was 70.37%, and there were no significant differences in the blood routine examination, liver and renal function before and after 72 hours′treatment of linezolid（P>0.05）.Conclusion　Linezolid has conspicuous effectiveness and safety in the treatment of severe sepsis within 72 h in intensive care unit.]]> 2017/12/6 8:06:37 XIANG Shu-lin, XIONG Bin 513true ［Abstract］　Objective　To study the correlation between semen quality, sperm DNA integrity rate（DNA fragmentation index, DFI ） and working hours in infertility male drivers.Methods　150 infertility male patients(including 110 occupational drivers, 40 patients who never drove), were divided into 3 groups according to the driving hours every day: group A with driving 1～5 hours every day(n=50); Group B with driving >5 hours every day(n=60); Group C without driving every day(n=40).The semen quality and sperm DNA integrity rate were analyzed in the patients.Results　(1)The quantity, vitality, concentration of sperms and PR in group C were higher than those in group A, B respectively［(3.10±1.32）ml vs (2.16±0.88)ml, (1.27±0.72)ml; (39.01±9.01)% vs (27.38±6.00)%, (21.34±7.95)%; (25.80±17.92)10⁶/ml vs (12.60±4.59)10⁶/ml, (7.66±2.92)10⁶/ml; (5.35±1.89)% vs (3.06±0.68)%, (2.10±0.82)%］, P=0.000. (2)The motility and normal morphology of the sperms and PR in group A were higher than those in group B［(2.16±0.88)ml vs (1.27±0.72)ml; (27.38±6.00)% vs (21.34±7.95)%; (3.06±0.68)% vs (2.10±0.82)%］, P=0.000. There was no significant difference in the sperm concentration between the two groups［（12.60±4.59）10⁶/ml vs (7.66±2.92)10⁶/ml］. (3)DFI(%) of group B was higher than that of group A and group C［(21.54±12.28)% vs (13.00±8.29)%, (10.15±6.75)%］, P=0.000; DFI(%) was not significantly different between group A and group C［(13.00±8.29)% vs (10.15±6.75)%］, P=0.172.Conclusion　Male drivers have poorer quality or quantity in sperm concentration, vitality, normal form and DNA integrity than the male controls who never drive and the longer time the male drivers drive, the poorer quality of the sperms they will have.]]> 2017/12/6 8:06:37 HE Yong-zhi, LI Da-wen, HE Bing, et al. 512true ［Abstract］　Objective　To evaluate velopharyngeal closure situation in children with postoperation of the cleft palate who speak Guangxi Cuengh language using nasopharyngeal fiberscope(NPF) and analyse the rate of velopharyngeal incompetence(RVPI), detect speech intelligibility(SI) and observe the situation of speech improvement after cleft palate surgery and to give target treatments for improving the level of cleft palate treatment.Methods　Twenty children with cleft palate(10 boys, 10 girls, 3～6 years) who speak Cuengh language were divided into two groups. They were examined using NPF respectively after 3 or 6 months′ operation. Velopharyngeal closure function was recorded respectively when pronounced a, e, i, o, u, w or not. SI was analyzed.Results　The children who pronounced the cuengh(a, e, i, o, u, w) after 6 months，training had significantly lower RVPI(a, e, i, o, u, w) than they did after 3 months, training(P<0.05). Compared with preoperative SI, the postoperative SI was significantly different(P<0.001). There was no obvious difference of SI between 3 and 6 months after the operation(P>0.05).Conclusion　With systemic speech trainings and effective human interventions, the RVPI and SI could be improved to some extents for children with cleft palate.]]> 2017/12/6 16:26:21 LI Bo-li, QIN Shi-yu, WANG Xiao-meng, et al. 511true ［Abstract］　Objective　To evaluate the effect of complex of casein phosphopeptide and amorphous calcium phosphate(trade name: GC Tooth Mousse) application in enamel remineralization in vitro.Methods　The in vitro premolars enamel blocks were made as specimens. After weighing, the specimens were soaked with 37.5% phosphoric acid for 2 minutes. At 5 min after the dry the specimens were weighed with a balance. Then after acid etching Tooth Mousse was used to coate the surface of acid etching tooth blocks for 30 min, immersed in distilled water for 1 hours, weighing at 5 min after the dry. According to different time the specimens were divided into the A～L of 12 groups. Statistical analysis was performed in the relation between the weight and the soaking time of each experimental group and the acid etching group.Results　The statistical analysis showed there was no statistical difference between the groups after soaking and the control group in average weight(P>0.05), and between the experimental groups there was no a statistically significant difference(P>0.05).Conclusion　The remineralization effect of Tooth Mousse is not straight up but a curve.]]> 2017/12/6 16:26:21 CHEN Yan, ZHOU Yan, LI Shi-Jian, et al. 510true ［Abstract］　Objective　To observe the homozygous deletions of RB1CC1 gene of disseminated cells in bone marrow of mouse oral carcinoma model in the severe abnormal hyperplasia stage.Methods　The cytokeratin positive(CK+) cells from 35 single nuclear cells smear of bone marrow of mouse oral carcinoma model in the severe abnormal hyperplasia stage were isolated by Laser Capture Microdissection(LCM). The DNA of a single cell was amplified by the technique of single-cell Whole Genome Amplification(WGA) and the homozygous deletions of RB1CC1 gene of the DTC were detected by Polymerase Chain Reaction(PCR). Four normal, 4 severe abnormal hyperplasia and 4 squamous cell carcinoma tongue were taken as control.Results　Thirty five CK+ cells from the 35 smear respectively were successfully isolated by LCM. Genomic DNA were successfully amplified by single-cell WGA in 3 out of 35 cells. Homozygous deletions of RB1CC1 gene were found in 2 of the 3 CK+ cells and 2 in 4 cases of squamous cell carcinoma tongue mucosa. However, the deletion was not found in the rest tissues.Conclusion　Homozygous deletion of RB1CC1 gene of the CK+ positive cells in the severe abnormal hyperplasia stage may be the disseminated tumor cells.]]> 2017/12/6 16:26:21 SHI Qiang, MU Yun, YU Da-hai, et al. 509true ［Abstract］　Objective　To observe and explore the characteristics of ultrasonography for transrectal color Doppler ultrasound in the diagnosis of hypertophy of the prostate disease and specific antigen(PSA) and image features in clinical stages of prostate cancer(PCa) systematically.Methods　Patients clinically suspected of PCa underwent prostate exam by transrectal color doppler ultrasound, then by transrectal ultrasound guided prostate biopsy by 6 points adding suspected nodes method, comparing with the PSA, pathologic results, PPBCs, Gleason score, and clinical stages.Results　In 413 patients confirmed by pathology(149 PCa, 171 BPH, 44 BPH with inflammation, 49 BPH with PIN and AAH), prostate disease had some characteristic ultrasound images. Clinical stages A,B,C or D(Whitmore-Jewett methods) of PCa had some different characteristics of ultrasound images. The results showed that PSA, PPBCs and Gleason grades were positively correlated with PCa stages respectively.Conclusion　Transrectal color Doppler ultrasound not only clearly reveals the internal structure of prostate,but also has the characteristic ultrasound imaging.Combination of image features with PSA is helpful for improving the diagnosis of prostate disease.]]> 2017/12/6 16:26:21 HE Yan, WANG Xiao-yan, LING Bing, et al. 508true ［Abstract］　Objective　To study the characteristics of cognitive function in primary school children with learning disorder to find out a solution.Methods　The comparison research method and the cognitive assessment system(DN: CAS) were used to test learning disorder children(n=32) and normal children(n=32).Results　There were significant differences in the scores of simultaneous processing and attention points between learning disorder children groups［（33.86±6.89）,（25.41±7.56）］ and normal children group［（40.55±5.25）,（34.05±8.97）］. The specific performances included the disorders of speech space relation, graphics memory, digital testing and acceptance notice. Multiple regression analysis showed that total scale score of learning disorder children′s academic performance had significant predict effect, explaining the amount of 27.8%.Conclusion　Learning disorder children have cognitive defects. Their simultaneous processing and attentions are significantly lower than normal children.]]> 2017/12/6 16:26:21 LI Xiao-long, WU Xin, CHEN Cui-hua, et al. 507true ［Abstract］　Objective　To evaluate the value of endoscopic ultrasonography(EUS) in the diagnosis and endoscopic therapy of colorectal cancer.Methods　A total of 23 cases were treated by different endoscopic theraputic techniques according to the results of EUS. The specimens were routinely diagnosed by pathology. The sensitivity, specificity, positive predictive value of EUS were evaluated according to pathological diagnosis as the gold standard.Results　The total accuracy of T- staging by EUS on colorectal cancer was 80.6%, among them, the accuracy of T1- staging by EUS on colorectal cancer was 100.0%, accuracy of T2- staging by EUS on colorectal cancer was 68.8%, and accuracy of T3- staging by EUS on colorectal cancer was 90.0%, respectively. The accuracy of N- staging by EUS on colorectal cancer was 77.4%. The sensitivity and specificity of N- staging by EUS on colorectal cancer were 58.3% and 89.5%, respectively. The positive predictive value, negative predictive value and Youden index of N- staging by EUS on colorectal cancer were 77.8%, 77.3%, and 0.478, respectively.Conclusion　These results suggest that EUS have higher accuracy judgment on staging of colorectal cancer T1, T3, and N-staging by EUS checks provide guidance for selection of the treatment of colorectal cancer.]]> 2017/12/6 16:26:21 YU Zhi-jin, PENG Xiao-feng, CHEN Hui-xin 506true ［Abstract］　Objective　To investigate the changes of plasma L-carnitine levels in the patients with multiple myeloma(MM) before and after the chemotherapy and explore their clinical significances.Methods　The levels of plasma L-carnitine of 10 healthy people and 36 MM patients(21 initially-diagnosed and 15 relapsed patients with MM) were tested. All patients were given chemotherapy with or without anthracyclines and the levels of plasma L-carnitine were tested by high performance liquid chromatography.Results　The levels of plasma L-carnitine of initially-diagnosed and relapse patients with MM were lower than those of healthy people, but the levels of relapsed MM were lower than those initially-diagnosed MM(P<0.05）; Those levels of plasma L-carnitine of MM one day and one week after chemotherapy were lower than those before chemotherapy. Chemotherapy with anthracyclines can reduce the levels of plasma L-carnitine of MM patients (P<0.05）.Conclusion　Chemotherapy could ruduce the levels of plasma L-carnitine of MM patients,indicating that it could be important to supplement L-carnitine during the chemotherapy.]]> 2017/12/6 16:26:21 XU Li, MA Na, LI Yong-gan, et al. 505true ［Abstract］　Objective　To study the changing trend of chronic disease and its related risk factors in Nanning community residents.Methods　The certain community residents in Nanning were investigated using the same questionnaire in 2007 and 2012, respectively. The survey items included smoking, drinking risk factors, and physical examination. The comparative analysis was done statistically.Results　In 2007, the prevalences of hypertension, hyperlipidemia, diabetes and stroke were 22.8%, 14.4%, 4.2% and 2.7% respectively; The current smoking rate, drinking rate over the past 12 months, central obesity rate, and obesity rate were 23.1%, 22.0%, 60.8% and 9.8% respectively. In 2012, the prevalence of hypertension, hyperlipidemia, diabetes and stroke were 23.2%, 19.4%, 8.3% and 2.8% respectively; The rate of current smoking, drinking rate over the past 12 months, central obesity rate, and obesity rate were 17.5%, 19.4%, 68.1% and 16.8% respectively. Compared with 2007, the hyperlipidemia and diabetes prevalence in 2012 were significantly elevated(χ²=4.536, 7.280, P=0.033, 0.007). Central obesity and obesity rates were on the rise significantly(χ²=5.655, 10.858, P=0.017, 0.001). Nevertheless, the current smoking rate was on the decline(χ²=4.779, P=0.029). Hypertension and stroke prevalence, drinking rate changed little(χ²=0.018, 0.017, 0.989, P=0.892, 0.898, 0.320).Conclusion　The prevalences of hyperlipidemia, diabetes, obesity, and central obesity are on the remarkable rise, while current smoking rate is on the decline a bit during the last 5 years in Nanning community residents. The proper diet and physical exercise such as “eat-move balanced” behavior intervention should be better to control the hyperlipidemia, diabetes, and obesity. More measures should be taken for tobacco and alcohol control.]]> 2017/12/6 16:26:21 ZHOU Fang-hua, ZHOU Ji, YE Lin, et al. 504true ［Abstract］　Objective　To explore the effect of methylprednisolone on the levels of cluster of differentiation (CD)80 and CD86 in the central nervous system of mice with experimental autoimmune encephalomyelitis(EAE).Methods　The EAE model was induced in mice by immunized with myelin oligodendrocyte glycoprotein peptides(MOG35-55). 30 female C57BL/6J mice were randomly divided into 3 groups: methylprednisolone-treated group, EAE group and normal group. The brain tissues were collected and their expressions of CD80 and CD86 were detected using western blot.Results　The neurological deficits of the methylprednisolone-treated mice were lighter than those of the EAE mice(P<0.05), while the methylprednisolone-treated mice displayed the lower expression of CD80 and the higher expression of CD86 than the EAE mice did(P<0.05).Conclusion　Methylprednisolone can protect against EAE in mice by the inhibition of CD80 and upregulation of CD86.]]> 2017/12/6 16:26:21 LI Lv-li, WEI Jun-jie, LI Xiao-feng, et al. 503true ［Abstract］　Objective　To analyze the relationship between gene interleukin(IL)-1β-511, interleukin gene(IL)-10-592 polymorphism and genetic susceptibility of pneumoconiosis in coal miners in Guangxi.Methods　A questionnaire surrvey of occupational history, history of pneumoconiosis etc, was conducted among 88 pneumoconiosis patients and 44 dust-exposed workers. The peripheral venous blood was collected in each studied object, and polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) technique was used to detect polymorphism of interleukin gene IL-1β-511 and IL-10-592.Results　Eighty-eight pneumoconiosis patients and 44 dust-esposed workers were the workers in a Bureau of Mines in Guangxi. Among 88 pneumoconiosis patients, there were 64 patients with silicosis who were exposed primarily to silica dust; 24 patients with coal workers′ pneumoconiosis who were exposed primarily to coal dust. Electrophoresis showed that wild-type gene of IL-1β-511 in silicosis group was 13 cases, 50 cases of mutant and wild type gene of IL-10-592 was 35 cases, 29 cases of mutant; wild-type gene of IL-1β-511 in Coal workers′ pneumoconiosis group was 4 cases, 20 cases of mutant and wild type gene of IL-10-592 was 15 cases, 9 cases of mutant; wild-type gene of IL-1β-511 in the control group was 17 cases, 27 cases of mutant; wild type gene and mutant of IL-10-592 both were 22 cases. In comparison of IL-1β-511, IL-10-592 allele frequency in Coal workers′ pneumoconiosis group and silicosis group comparison with that in the control group, the difference was not statistically significant(P>0.05).Conclusion　The interleukin gene IL-1β-511 and IL-10-592 gene polymorphism may be irrelevant to the incidence of Guangxi mine workers′ pneumoconiosis.]]> 2017/12/8 8:10:56 MAI Zhi-dan, LI Xiao-ping, LIANG Mei, et al. 502true ［Abstract］　Objective　To study safety and economic effectiveness of very early extubation in children with atrial septal defect(ASD) or ventricular septal defect(VSD) after open heart surgery.Methods　Fifty cases of children with ASD or VSD were randomly divided into two groups, very early extubation group(group Ⅰ, n=25) and traditional eary extubation group(group Ⅱ, n=25). The two groups were compared with data on ICU observing time, hospitalization costs, blood gas analysis after tracheal extubation and pulmonary complications.Results　Blood gas analysis at the 30th min after tracheal extubation in group Ⅰwas similar to that in group Ⅱ(P>0.05). There were no significant differences between the two groups on the data on the rate of eintubation, time of postoperative hospital stay, pulmonary complication(P>0.05). The length of ICU stay and hospitalization costs in group Ⅰ were significantly less than those in groupⅡ(P<0.05).Conclusion　Very early extubation in children with ASD or VSD after open heart surgery is safe and economical.]]> 2017/12/8 8:10:56 SHI Qi-fang, LIANG Da-sheng 501true ［Abstract］　Objective　To evaluate the distribution of vessels inside and adjacent to lesion region at three-dimensional color histogram can be used for the differentiation of benign and malignant breast lesions.Methods　One hundred sixty five patients with breast lesions were divided into malignant lesion group(n=93) and benign lesion group(n=72), according to pathological findings. All cases underwent three-dimensional ultrasound exam. Three-dimensional color histogram was used to assess the volume and breast lesion indexes inside and outside 3 mm region, which included vascularization index(VI), flow index(FI）and vascularization-flow index(VFI ). Vascularity indexes inside were expressed respectively as V-in, VI-in, FI-in and VFI-in, and those outside surrounding region of 3 mm were expressed respectively as V-out, VI-out, FI-out and VFI-out. All the results were compared between malignant lesion group and benign lesion group.Results　VI-in, VFI-in, VI-out, FI-out and VFI-out in malignant lesion group were significantly higher than those in benign lesion group(P<0.05). The indices of V-in, V-out and FI-in in the two groups had no significant differences(P>0.05). Based on the receiver operating characteristic(ROC) curve, VI-out was the best index for the diagnosis of breast lesions. VI-in, VFI-in and VFI-out were the second one, and FI-out was the last one.Conclusion　The vascularity index of 3 mm surrounding region has certain value in the diagnosis of breast lesions. The quantitative analysis of three-dimensional color histogram has a certain value in assessing blood flow inside and adjacent to breast lesions and improves the differential diagnosis of benign and malignant breast lesions.]]> 2017/12/8 8:10:56 ZHANG Bing, WANG Xiao-yan, NONG Mei-fen, et al. 500true ［Abstract］　Objective　To explore the clinical manifestations and pregnant outcomes of the patients with gestational diabetes mellitus(GDM) for proventing possible poor situation.Methods　The clinical data of pregnant women with GDM(GDM group,n=138) and normal pregnant women during pregnancy(control group,n=138) were collected from January 2013 to January 2014 in the peoples of Guangxi Zhuang Autonomous Region Hospital. The pregnant outcomes and perinatal situation in both groups were analyzed.Results　The amniotic fluid index, maternal weight during pregnancy and neonatal birth weight in GDM group were higher than those in the control group with statistic difference(P<0.05). The ratio of cesarean section, the abnormal amniotic fluid, the early dilivery ratio of GDM group were higher than those of the control group(P<0.05); The pregnancy complications in GDM group, such as gestational hypertension, neonatal complications and the incidence of postpartum hemorrhage, were much higher than those in the control group.Conclusion　GDM patients have significantly elevated indexes than those of normal pregnant women, so have different pregnant outcomes. Prevention states should be adopted to reduce the risk of GDM related complications.]]> 2017/12/8 8:10:56 ZHANG Chun 499true ［Abstract］　Objective　To explore the clinical value of combined application of micro-gel method and glycine/EDTA diffuse method in the diagnosis of hemolytic disease of the newborn(HDN) caused by maternal-fetal blood type incompatibility.Methods　Micro-gel method and glycine/EDTA diffuse method were jointly used to detect 355 blood samples from newborn with suspected HDN caused by maternal-fetal blood type incompatibility.Results　HDN caused by maternal-fetal blood type incompatibility was diagnosed in 277 cases, the positive rate was 78.03%, of which Rh-HDN was detected in 2 cases(0.72%), ABO-HDN in 275 cases(99.28%). In 275 cases of ABO-HDN, mother and baby were both blood type O/A accounted for 48.73%, O/B accounted for 51.27%, no B/AB and A/AB blood type. For these newborn with jaundice between 2 h≤n≤3 d,3 d<n ≤5 d,5 d<n ≤7 d,7 d<n≤13 d after birth. HDN positive detection rates were 86.10%, 73.02%, 31.58%, 14.29%, respectively.Conclusion　Joint application of micro-gel method and glycine / EDTA diffuse method in the detection of HDN, can accurately and timely provide the basis for clinical diagnosis and treatment.]]> 2017/12/8 8:10:56 LI Hai-lan, JIAO Wei, LI Ying-lan, et al. 498true ［Abstract］　Objective　To investigate the clinical effect and safety of low molecular weight heparin(LMWH) for unexplained early recurrent spontaneous abortion(RSA).Methods　One hundred patients with unexplained RSA were randomly divided into treatment group and control group, with 50 cases in each group. Both groups began to receive drugs treatment from early pregnancy, usually after pregrancy was diagnosed by the blood β-HCG or intrauterine pregnancy was dinenosed by ultrasound examination. The control group received the following therapy: progesterone 20 mg, intramuscular injection, once a day; chorionic gonadotropin 1 000 U, intramuscular injection, once a day until 12 weeks of pregnancy. The treatment group was given low molecular weight heparin 5 000 U pariumbilical subcutaneous injection once a day based on the above treatment.After treatment, the pregnancy outcomes, drug side effects and neonatal malformations were observed in the two groups.Results　The live births rate in the treatment group was 90%, that in the control group was 62%, the difference in the live births rate between the two groups was statistically significant(P<0.05); the incidence of adverse drug reactions in the control group was 74%, higher than that（24%） in the treatment group, the difference was statistically significant(P<0.05); there were no neonatal malformations in the two groups.Conclusion　LMWH can significantly improve the pregnancy outcome of patients with unexplained early recurrent spontaneous abortion and has no adverse effects on the newborns.]]> 2017/12/8 8:10:56 LIANG Qing-fen 497true ［Abstract］　Objective　To evaluate the diagnostic value of multiple slice spiral CT portal venography(CTPV) in the collateral circulation of liver cirrhosis with portal hypertension.Methods　Tri-phase enhanced CT scan was performed in 109 patients with portal hypertension dut to cirrhosis.The diagnosis was proved by clinical data,hepatic function findings and imaging signs.Three dimension imaging reconstruction of portal venous system and portal collateral circulation were obtained using posl-processing reconslruction technique.Results　CTPV images displayed the portal venous system and its collateral circulation stereoscopically. Of 109 patients, left gastric varices were seen in 67（61.5%）, lower esophageal varices in 87（80.0%）, short gastric or posterior gastric varices in 10 （9.2%）, paraesophageal varices in 21（19.3%）, gastro-renal or splenorenal shunts in 14（12.8%）, spongelike transformation of portal vein in 18（16.5%）, paraumbilical and abdominal wall varices in 15（13.8%）, paravertebral venous shunts in 6（5.5%）.Conclusion　Multiple slice spiral CT can precisely display the location, extent and course of the collateral circulation. It can provide reliable imaging evidence for clinical evalution before treatment.]]> 2017/12/8 8:10:56 LI Rui-xiong, JIANG Shi-wu, YANG Wei-zhen 496true ［Abstract］　Objective　To investigate the effects of cognitive dysfunction on the quality of life in patients with hepatocellular carcinoma and the corresponding nursing strategies.Methods　One hundred twenty seven patients with hepatocellular carcinoma were screened with the mini-mental state examination and SF-36 health survey questionnaire. The patients were divided into the observation group(hepatocellular carcinoma with cognitive dysfunction, n=36) and the control group(hepatocellular carcinoma without cognitive dysfunction, n=91), and the SF-36 scores were compared between the two groups.Results　There were no significant differences in gender, age, body mass index and other general conditions between the two groups(P>0.05). The physical function, role-physical, bodily pain, social functioning, mental health, role-emotional, vitality and general health were significantly lower in the observation group than those in the control group(P<0.05).Conclusion　The quality of life of the hepatocellular carcinoma patients with cognitive dysfunction is worse. The active nursing intervention should be conducted to reduce the cognitive dysfunction and improve the quality of life in those patients.]]> 2017/12/8 8:10:56 LV Jing, CHEN Si-xia, LI Jia-mei, et al. 495true ［Abstract］　Objective　To compare the effect and value between magnetic resonance sialography(MRS),CT siaglography and X-ray sialography in the diagnosis of Sjögren′s syndrome.Methods　Three hundreds and one suspected Sjögren′s syndrome,of them 65 cases took MRS,130 cases took CT sialography and 106 took X-ray sialography. And these results were compared.Results　These three methods′ sensitivity, specificity and Youden index were as following: MRS:95%, 80%, 0.75, CT sialography: 87.5%, 80%, 0.675, X-ray sialography: 72%, 90%, 0.62 respectively. The area under the ROC curve of MRS, CT sialography and X-ray sialography were 0.875,0.837,0.815 respectively.Conclusion　MRS′s accuracy in the diagnosis of Sjögren′s syndrome is higher than the conventional X-ray sialography, and it is noninvasive and has high clinical value.]]> 2017/12/12 9:29:09 WEI Jie-qin, ZHANG Jing, LU Li-jian, et al. 494true ［Abstract］　Objective　To analyse the cognitive assessment damage situation of children with Asperser′s syndrome(AS), our goal is to contribute to the diagnosis and treatment of AS.Methods　Nineteen children met the diagnostic criteria of AS were collected as AS group. And 19 healthy children were selected as normal control group. The Das-Naglieri cognitive assessment system(DN-CAS) was adopted to evaluate the two groups of children, the characteristics of cognitive function were statistically analyzed and compared between the two groups.Results　AS children′s scores in Sohn Grayson AS rating scale were at 119～191(including 12 mild cases, 4 moderate cases and 3 severe cases). AS children′s DN-CAS scores, planning ability, attention and simultaneous processing capacity were significantly lower than those of normal children(P<0.05).Conclusion　AS children′s planning ability, attention and simultaneous processing capacity were abnormal, and their general cognitive levels were lower than normal children.]]> 2017/12/12 9:29:09 CHEN Cui-hua, WU Xin, LIN Jing-wei, et al. 493true ［Abstract］　Objective　To explore the action mechanism of extracorporeal shock wave combined with Chinese herbal medicine(veronica peregina L) in the treatment on rabbit bone nonunion.Methods　Sixty-four standard experiment New Zealand white rabbits were chosen, the rabbit models of radial bone nonunion were made under aseptic conditions by osteotomy, then randomly divided into A, B, C, D group. Group A was treated by extracorporeal shock wave combined with Chinese herbal medicine(veronica peregina L); Group B was treated by bone Veronica peregrina L; Group C was treated by extracorporeal shock wave; Group D was control. The generation of bone callus of each group before and after the treatment were observe and compared, and statistical analysis was performed.Results　The generation of bone callus: the comparision among groups of the bone callus after the treatment at each time point the difference was statisticlly significant(P<0.01); In group A, the generation of bone callus after the treatment by extracorporeal shock wave was higher than group C at 2, 4, 6, 8 weeks, the difference was statistically significant（P<0.01）; Group A was higher than group D in the generation of bone callus, the difference was statistically significant（P<0.01）; The generation of bone callus at each time point between goup B and group D did not show significant difference（P>0.05）.Conclusion　Veronica peregrina L can accelerate fracture healing after bone nonunion treated by extracorporeal shock wave in the early time that provides a good method for the non-surgical treatment of bone nonunion.]]> 2017/12/12 9:29:09 LI Hong-yu, LAI Wen-bing, YU Shao-lin, et al. 492true ［Abstract］　Objective　To detect the change of MMP-3 in the prominent intervertebral disc tissue and blood in rats of intervertebral disc herniation to explore the feasibility of conservative treatment for intervertebral disc herniation with MMP-3.Methods　Forty SD rats(only 3 months of age) were randomly divided into normal control group, negative control group, experimental group 1 and experimental group 2, 10 in each group. The MMP-3 content in disc herniation organization and the blood were detected with enzyme-linked immunosorbent assay method after 45 days of experiment.Results　(1)The expression of MMP-3 was significantly higher in the experimental group 1 and group 2 compared with that of normal control group(P<0. 05); (2)The express of MMP-3 was significantly higher in group 1 compared with that of group 2(P<0. 05); (3)the concentration of MMP-3 between intervertebral disc tissue and blood in group 1 and group 2, had a linearly relating, and the correlation coefficients were 0.835, and 0.924(P<0. 05).Conclusion　The content of MMP-3 in the blood rises in the process of intervertebral disc herniation resorption, and content of MMP-3 in local intervertebral disc tissue is highly correlated with the level of that in the blood, idea of nonsurgical treatment by local injections with MMP-3 to cure intervertebral disc herniation may not come true in the clinical use.]]> 2017/12/12 9:29:09 QIN Jin-ding, JIANG Yao-chuan, XIAO Rong-chi 491true ［Abstract］　Objective　To explore the effect and action mechanism of extracorpoporeal shock wave combined with hyperbaric oxygen in the treatment of bone ununion.Methods　Forty-eight health standard experiment New Zealand white rabbits were chosen(months of age are consistent with weights), and divided into four groups by using the random number table after the models were made by osteotomy(12 in each group). The group A was extracorporeal shock wave combined with hyperbaric oxygen group; The group B was hyperbaric oxygen group; The group C was pure extracorporeal shock wave group; The group D was in control group, X-ray examination was made before treatment and at 2, 4, 8, 12 weeks after treatment; and part of the experimental animals were executed at each time point to get the live tissue for making general observation, observing the change of nonunion gap, detecting the calcium content, using the optical microscope to observe the osteoblast in bone callus, and statistical analysis of the data was made at last.Results　X-ray obseversion showed that there was a significant difference in the nonunion gap at 4, 8, 12 weeks between group A and group B, C or D（P<0.05）. Callus calcium contents at 2, 4, 8, 12 weeks in group A were higher than those in group C, the difference was statistically significant（P<0.05）.There was a significant difference in callus osteoblast count at 2, 4, 8 weeks between group A and group C(P<0.05).Conclusion　Extracorporeal shock wave combined with hyperbaric oxygen treatment for bone nonunion is better than pure extracorporeal shock wave therapy, simple hyperbaric therapy has no obvious help for the treatment of bone nonunion, hyperbaric oxygen can be used as a good synergy method in the extracorporeal shock wave treatment of bone nonunion.]]> 2017/12/12 9:29:09 YU Shao-lin, LI Hong-yu, LIU Bo-yu, et al. 490true ［Abstract］　Objective　To explore the conditions of syphilis infection among maternal women in Guangxi and provide scientific evidence for making policy to intervene in mother-to-child transmission of syphilis.Methods　Rapid plasma Reagin Test(RPR) and Toluidine Red unheated Serum Test (TRusrt) were used to screen syphilis infections in the prenatal maternal and hospital delivery women. Trepmen Pauidum Particle Agglutination(TPPA) was applied to confirm the results.Results　2 575 233 pregnant women were included in the study. The testing numbers were 798 305, 924 622 and 852 306 respectively from 2011 to 2013 in Guangxi. The detection rates had a rising trend year by year which were 96.12%, 99.55% and 99.84% from 2011 to 2013, the positive rates were 0.22%, 0.09% and 0.17% respectively from 2011 to 2013. The positive rate in 2013 was twice as many as that in 2012. The proportion of syphilis detection in pregnant women was 24.03% higher in 2013 than that in 2011. The proportion of intrapartum detection was decreased by 78.26% in 2013 compared with that in 2011. The positive rates of intrapartum detection were respectively 0.47%, 0.55% and 0.67% from 2011 to 2013.Conclusion　Maternal syphilis infection has increased year by year in Guangxi. It is necessary to strengthen standard treatment of the maternal syphilis infection and their birth-infant, and follow-up on a regular basis and scientific management to control the syphilis mother-to-child transmission.]]> 2017/12/12 9:29:09 HUANG Yue-hua,QIU Xiao-xia,LI Ying, et al. 489true ［Abstract］　Objective　To evaluate the efficacy and application principles of one-stage posterior intervertebral debridement, bone graft and internal fixation for the treatment of lumbosacral tuberculosis.Methods　A retrospective analysis was performed on the clinical data of 15 cases of lumbosacral tuberculosis who were treated by surgery and got complete follow-up data. The changes of Cobb angle, the conditions of neurological function recovery, and the time of postoperative bone fusion before and after surgery were observed.Results　Fifteen patients were followed up for 1～5 years, with an average of 3 years. All patients′ incisions were primary healing. The postoperative X-ray films showed good bone graft and internal fixator position, and grafts gained bone fusion during 3.5 to 7 months. No loosening and fracture of internal fixator was found. Cobb angle was averagely 14 degrees. No significant loss of corrective angle was found, spinal cord function recovered well. According to Frankel grade, in 4 patients with grade C before surgery, postoperative spinal cord function restored to grade D in one case, E grade in 3 cases, in 11 patients with grade D before surgery, postoperative spinal cord function retured to grade E. No recurrence of tuberculosis was found.Conclusion　One-stage posterior intervertebral debridement, bone graft and internal fixation for the treatment of lumbosacral tuberculosis has advantages of less damage, better bone graft stability, higher fusion rate, effective correction of kyphosis deformity, and better therapeutic results.]]> 2017/12/12 9:29:09 LIU Chang-sheng, JIA Shi-qing, NING Yun-qian, et al. 488true ［Abstract］　Objective　To apply PCR-DGGE technology analysing changes of oral microbial floras in children with hand foot and mouth disease(HFMD).Methods　According to the random number table method,3 children with HFMD were randomly chosen and their saliva samples were collected;at the same time, saliva samples were collected from one normal child,and from one child with herpetic ginggivostomatitis(HGS). Samples′ total DNA extraction of the microbial community were performed; taking it as template for amplification of 16 s rRNA V3 variable area, the structure of product by the DGGE fingerprint, and using UVIBAND/MAP software for comparing similarity index of the community fingerprint; the edge banding on swimming belt of DGGE fingerprint were excised from the gel for sequencing, gene sequence analysis was performed, then the sample advantage bacterium group was obtained.Results　The advantage bacterial colonies of HFMD group: Capnocytophaga, Selenomonas, Prevotella, TM7_genera_incertae_sedis, Streptococcus,Neisseria,Porphyromonas,Campylobacter,Campylobacter,Granulicatell-a, Leptotrichia, Veillonella, Bacteroides-like sp, Actinomyces, Fusobacterium. The advantage bacterial colonies of normal group: Veillonella, Capnocytophaga, Prevotella,Gemella,Porphyromonas,Ruminococcaceae,Lachnospiraceae, Selenomonas, Clostridium, Haemophilus. The advantage bacterial colonies of HGS group: Lactococcus,Prevotella.Conclusion　The flora structure of HFMD group was more complex, and there was obvious difference in the flora structure between HFMD group and HGS group.]]> 2017/12/12 9:30:01 SHI Chun-mei, ZHOU Yan, FANG Bang-qian, et al. 487true ［Abstract］　Objective　To analyze the characteristics of type-C behavior among non-small cell lung cancer(NSCLC) patient in Guangxi,and to provide scientific evidences for prevention and treatment of non-small cell lung cancer effectively.Methods　A type-C behavior was investigated with a questionnaire among 202 NSCLC in patients(NSCLC group) and 202 non-tumor in patients(control group) at the First Affiliated Hospital of Guangxi Medical University from September 2012 to March 2013.Results　The scores of anxiety,depression,anger,intrinsic anger,reason and control in the NSCLC group were higher than those in the control group;but the extrinsic anger,optimist mood and social support in the NSCLC group were lower than those in the control group,with significant difference between the two groups(P<0.01). For different sex, the scores of anxiety,depression,anger,intrinsic anger,reason and control in the NSCLC group were both higher than male or female norm,but the scores of optimist mood and social support in the NSCLC group were both lower than male or female norm(P<0.01).Conclusion　The type-C behavior in NSCLC patients is obvious in Guangxi,and it reveals that the type-C behavior is related to occurrence and development, and specific preventive measures should be taken in different populations.]]> 2017/12/12 9:30:01 PENG Guo-qing, XIAN Lei 486true ［Abstract］　Objective　To explore the objectivity and sensitivity of multifocal visual evoked potential(mfVEP) combined with optical coherence tomography(OCT) in the diagnosis of functional and structural change of glaucoma.Methods　Ninety glaucoma patients(experimental group) and 30 patients with normal eyesight(the control group) were taken Humphrey perimetry(HVF), mfVEP and OCT examination.According to the extent of glaucomatous visual field damage,all glaucoma patients were divided into 3 subgroups: mild damage group, moderate damage group, severe damage group,with 30 cases in each group. One eye with lighter damage was selected for evaluating the sensitivity and objectivity of mfVEP and OCT.Results　The average visual field defect(mean deviation, MD) was (-0.93±0.97)dB in the control group, (-3.16±1.76)dB in mild injury group, (-12.82±3.92)dB in moderate damage group, (-27.10±3.73)dB in severe damage group.The extent of damage in each subgroup of the experimental group was greater than that in the control group,showing a statistically significant difference; The average thickness of peripapillary retinal nerve fiber layer was (109.14±6.48)μm in the control group, (98.97±15.43)μm in the mild damage group, (72.63±11.21)μm in the moderate damage group, and (64.15±11.45)μm in the severe injury group, peripapillary RNFL thickness in each subgroup of experimental group was thinner than that in the control group,the difference was statistically significant, and RNFL thickness got more thinner with the increase of the extent of damage of glaucoma.The RMS of amplitude of mfVEP was (12.60±4.19)nV/deg² in the control group,(11.23±6.36)nV/deg² in the mild damage group, (10.38±4.95)nV/deg² in the moderate damage group, and (8.03±3.89)nV/deg² in the severe injury group, in all subgroups of experimental group,the difference between mild and moderate damage group and control group was not statistically significant, the difference between severe damage group and the control group was statistically significance.The average latent time of mfVEP was (104.37±24.95)ms in the control group, (102.09±32.89)ms in the mild injury group,(99.73±7.83)ms in the moderate damage group, and (96.71±5.86)ms in the severe damage group, between each subgroup of experimental group,and the control group the difference was not statistically significant.Conclusion　OCT can show the damage of the nerve fiber layer in patients with glaucoma objectively, mfVEP is more valuable for diagnosis of advanced glaucoma, and the RMS is more valuable than the average of latent time; mfVEP combined with OCT and HVF is more helpful in the diagnosis of glaucoma.]]> 2017/12/12 9:30:01 CHEN Li-fei, WEN Mei-dan, ZENG Si-ming 485true ［Abstract］　Objective　To investigate the local immune function and prognosis in patients with nasopharyngeal carcinoma(NPC) treated with p53 gene therapy.Methods　Sixty-three cases of advanced nasopharyngeal carcinoma were divided into p53 therapy group(n=32) and conventional therapy group(n=31). The p53 therapy group was treated by concurrent radiotherapy and chemotherapy plus nasopharynx intratumaral injection of rAd-p53;the conventional therapy group was treated by concurrent radiotherapy and chemotherapy. The expression of CD3 and CD20 were examined by immunochemistry and their relationship with prognosis was also analyzed.Results　The expression of CD3 was significantly increased after p53 gene treatment. There was no significant change in expression of CD20 after p53 gene treatment. Five-year disease-free survival rate in the p53 therapy group was higher than that in the conventional therapy group(P<0.05). Recurrence and metastasis rate was significantly different between p53 therapy group and the conventional therapy group(P<0.05).Conclusion　T cells are the most important proliferative cell of tumor infiltrating lymphocyte in NPC patients after p53 gene therapy. The local cellular immune status in NPC patients is improved after p53 gene therapy with decrease of tumor recurrence rate and metastasis rate. Gene therapy combined with radiotherapy and chemotherapy may help to improve the prognosis of patients with NPC.]]> 2017/12/12 9:30:01 QIN Yang-da, WENG Jing-jin, SI Yong-feng, et al. 484true ［Abstract］　Objective　To observe the clinical therapeutic effect of minimally invasive needle knife treatment of chronic knee periarthritis.Methods　By semi-randomized method,200 cases of chronic knee periarthritis were divided into observation group and control group, 100 cases in each group. The observation group received needle knife minimally invasive lysis treatment after nerve block, while the control group only received the treatment of nerve block. Before treatment and at 1 w, 2 w and 4 w after treatment resting pain VAS score(rest visual analogue scale,RVAS) and the passive activity VAS score(passive visual analogue scale,PVAS) were compared between the two groups;and the comprehensive curative effect was compared between the two groups.Results　After treatment, RVAS score and PVAS score in the two groups decreased, and the overall score of the observation group was lower than that of the control group, with significant difference(P<0.05).Compared with the control group, pain RVAS score and PVAS score at 2 w and 4 w in the observation group were significantly decreased after treatment(P<0.05). The therapeutic effect of observation group was better than that of the control group(P<0.01).Conclusion　The minimally invasive needle knife treatment has a significant efficacy in the treatment of chronic knee periarthritis,and its long-term efficacy is superior to single nerve block treatment.]]> 2017/12/12 9:30:01 LI Feng, LIANG Qi-rong, LI Fen, et al. 483true ［Abstract］　Objective　To observe the changes of right ventricular function in patients with coronary artery heart disease(CHD) before and after percutaneous coronary intervention(PCI).Methods　TA Sa, Tei index, TAPSE and RVFAC were evaluated in 77 patients with CHD before and after PCI. Additionally, TA Sa, Tei index, TAPSE and RVFAC were evaluated in 64 health volunteers.Results　Before intervention, TA Sa and TAPSE of patients with CHD were less than those of the health volunteers, Tei index was more than those of the health volunteers, and the differences were statistically significant(P<0.05). After intervention, TAPSE of patients with CHD was more than before,Tei index of patients with CHD was less than before, and the differences were statistically significant(P<0.05);TA Sa and TAPSE of patients with CHD were less than those of the health volunteers, Tei index was more than those of the health volunteers, and the differences were statistically significant(P<0.05).TA Sa had a close correlation with TAPSE(r=0.641, P<0.01),TA Sa and TAPSE had a close correlation with Tei index(r=-0.542, P<0.01;r=-0.570, P<0.01).Conclusion　TA Sa and TAPSE have a close correlation with Tei index, playing a significant role in estimating the short-term changes of right ventricle function before and after PCI in patients with CHD.]]> 2017/12/12 9:30:01 LIANG Mei-xin, LIN Jian-jun, LV Yong-cheng, et al. 482true ［Abstract］　Objective　To study the characteristics of the acute poisoning processes in Beihai city.Methods　By the epidemiological study, the 2 127 cases of all-kind-acute poisoning were surveyed from 10 hospitals of the city, county and township in Beihai city. The classification analysis was made by collecting the relevant data of poisoning processes of the cases.Results　In all the 2 127 cases, the numbers of poisoning in the poisoning sites of home, school, workplace, restaurants, entertainment and other places accounted for 86.63%,0.24%,5.37%,3.25%,1.46% and 3.06% of cases, through the poisoning ways of mouth, respiratory, skin for 68.47%,19.01%,12.52% of cases,and by the poisoning causes of accident, suicide, feeding, medicine, occupation, he-killed, and other for 36.05%, 25.65%, 15.20%, 7.06%, 2.45%, 0.24% and 13.35% of cases, respectively.In the results, the characteristics of poisoning processes of the home, mouth, accident and suicide were main. Further analyzing the causes and poisons of the characteristics, the numbers caused by the accident and pesticides in the home poisoning accounted for 33.33% and 29.18% of same cases, by the suicide and pesticides in the mouth poisoning for 36.70% and 31.99%, and by the chemistry and pesticides in the accident and suicide poisoning for 44.39% and 68.62% of same cases, respectively.Conclusion　The home, mouth, accident and suicide poisoning in Beihai city are main characteristics of the poisoning processes. The prevention and control countermeasures should be taken on the basis of these characteristics.]]> 2017/12/12 9:30:01 LIU Qing-hua 481true ［Abstract］　Objective　To construct the eukaryotic expression vector for the positive control plasmid of has-miR-335.Methods　One pair of complementary oligomeric chains of exact matching sequence for has-miR-335 seed region were synthesized，and then target fragments were obtained by annealing.The target fragments were inserted into pMIR-REPORT luciferase reporter vector.We cotransfected 293T/17 cell with the recombinant plasmid or pMIR-REPORT plasmid together with Pre-miR^TMmicroRNA335 Precursor by Lipofectamine 2000.The expression of has-miR-335 in eukaryotic cells was tested by Luciferase Assay.Results　The positive control recombinant plasmid of hsa-miR-335 was comfirmed by sequencing. The expression of has-miR-335 decreases after combined with it′s positive control in eukaryotic cells.Conclusion　The positive control plasmid for has-miR-335 can be used to confirm if the methods of experiments in RNA extraction,gene transfection and expression are reliable.The double luciferase reporter gene detection system of has-miR-335 is constructed successfully,which lays the foundation for testing and validation of has-miR-335 target genes.]]> 2017/12/18 8:27:00 WEN Zhi-hong, DAI Yan, HE Shuang 480true ［Abstract］　Objective　To study the safety and feasibility of Super Shear System used in laparoscopic right hemicolectomy.Methods　The clinical data of 36 patients undergoing laparoscopic right hemicolectomies using Super Shear System were analysed retrospectively between December 2010 and August 2014.Results　Thirty-six cases were selected in this study including 6 cases of cecum cancer，17 cases of ascending cancer and 13 cases of cancer of hepatic flexure of colon and all patients were performed successfully on surgery.The average operative time was (161.3±55.7)min. The mean volume of bleeding was (86.7±38.5)ml. Postoperative time of bowel gas passage was (3.2±0.8)d. The volume of abdominal drainage was (290.2±67.3)ml. No cmplications were experienced, such as ureteral injury, anastomotic leakage, and so on.The number of lymph node dissection was 5～21 and the mean was 12.6. The time of following up ranged from one month to forty-two months and the median value was twenty-three.seven cases were lost to follow-up.One case with stage Dukes C suffered anastomotic recurrence which was found by endoscopic examination and was confirmed by pathologic examination at postoperative ninth month, who refused any chemotherapy. Abdominal lumps were found in two cases with stage Dukes C by B-ultrasound and CT scan at postoperative sixteen month and twenty-three months, which were confirmed to be recurrent tumors. One case refused chemotherapy and another case received normal chemotherapy accordingly. Multiple metastasis in liver was found by B-ultrasound and CT scan in one case with stage Dukes C, who quited chemotherapy after two courses of treatments. Recurrence and metastasis were not found in other patients during following up.Conclusion　It is safe and feasible to perform laparoscopic right hemicolectomy using super shear system and super shear system is competent for laparoscopic right hemicolectomy.]]> 2017/12/18 8:27:00 FENG Ze-rong, LIANG Ping, WU Dong-bo, et al. 479true ［Abstract］　Objective　To apply systematic training of the perceptual learning based on internet for juvenile anisometropic amblyopia, understand and analyze the change in contrast sensitivity before and after the training.Methods　Two hundred and sixteen juvenile patients(216 eyes) with anisometropic amblyopia were chosen and trained with the perceptual learning system based on internet and the traditional combined training.The specific training programs were designed by doctor based on the patient initial vision, degree of severity in low vision and advancement during training process.The perceptual learning group received the visual trainings，such as improving visual noise，contour integration and position noise; the traditional combined training group received the visual trainings like red lights and fine vision training.Based on these two methods, the change in contrast sensitivity was analyzed before and after 3 months, 6 months, 9 months and 12 months′ training.Results　The CS Means of amblyopia from the perceptual learning group and the traditional combined training group decreased in each spatial frequency. The entire 3～18 c/d frequency difference had no statistical significance between two groups before treatment（P>0.05）. After the treatment, the entire CS（3～18 c/d） frequency difference in the perceptual learning group was higher than that in the traditional combined training group. The difference was statistically significant（P<0.05）. After the treatment, the entire 3～18 c/d frequency difference in the traditional combined training group was improved significantly(P<0.01).Conclusion　The perceptual learning based on internet can provide intensive，active，feedback and individual visual stimulus, thus being more effective than the traditional combined treatment for anisometropic amblyopia juvenile aged 9～13 who are out of vision sensitive developmental period. The new treatment can achieve better result in promoting the contrast sensitivity than the traditional combined treatment, thus providing a new feasible way for amblyopia clinical therapy.]]> 2017/12/18 8:27:00 LIN Quan, LIU Wei-Min, XIAO Xin, et al. 478true ［Abstract］　Objective　To detect the the level of IL-10 in bronchoalveolar lavage fluid and explore the diagnostic value of IL-10 in tuberculosis.Methods　The double-antibody sandwich ELISA methods were used to detect the expression of IL-10 in serum of 50 patients with pulmonary tuberculosis and 48 healthy people. The expression of IL-10 in bronchoalveolar lavage fluid of 50 patients with pulmonary tuberculosis was detected before anti- tuberculosis therapy and after 4 weeks′ therapy and compared their statistical differences.Results　The level of IL-10 in peripheral blood of patients with pulmonary tuberculosis was significantly higher than that of the healthy people. The level of IL-10 in both peripheral blood and in bronchoalveolar lavage fluid of patients with pulmonary tuberculosis decreased after anti-tuberculosis therapy than those before therapy.Conclusion　IL-10 plays an important role on immunosuppression in the the process of development of tuberculosis and it may assist in diagnosing tuberculosis in the clinical work.]]> 2017/12/18 8:27:00 WANG Juan, GE Hai-Bo, SUN Si-qing, et al. 477true ［Abstract］　Objective　To explore the correlation between main risk factors in second trimester pregnant women and preeclampsia(PE).Methods　Three hundred and twenty pregnant women were included in the study, and their general physical examination data were recorded,such as age,weight gain,mean arterial blood pressure etc.Automatic biochemistry analyzer was used to determinate the levels of serum total calcium(［TCa］_o) and ionized calcium(［Ca²⁺］_o). The suppression function of CD4⁺CD25⁺Tregs was detected by enzyme-linked immunosorbent assay.The pregnant women were followed up until delivery, and were divided into PE group and control group(pregnant women without PE). The correlation of these indexes and PE was observed.Results　(1)Follow-up outcomes: among 320 pregnant women,27 cases developed PE(PE group),the incidence was about 8.44%, including 21 cases of mild PE, 6 cases of severe; 293 cases had normal delivery(normal group).(2)Compared with the normal group, mean arterial blood pressure was higher in the PE group; serum ［TCa］_o, serum ［Ca²⁺］_o and Treg inhibition rate were lower(P<0.05).There were no significant differences in age, weight gain(P>0.05).In severe PE group serum ［Ca²⁺］_o was lower than that in mild PE group, the difference was statistically significant(P<0.05). (3)Correlation between levels of serum［TCa］_o,serum［Ca²⁺］_o and CD4⁺CD25⁺Treg inhibition rate: the Pearson coefficients of serum［TCa］_o,serum［Ca²⁺］_o and CD4⁺CD25⁺Treg inhibition rate were r₁=0.031,P=0.585;r₂=0.129,P=0.021, respectively, indicating that there was no correlation between TCa levels in serum and Treg inhibition rate but that there was a positive correlation between serum［Ca²⁺］_o and Treg inhibition rate.Conclusion　Insufficient serum［TCa］_o,serum［Ca²⁺］_o, deficient CD4⁺CD25⁺Treg inhibition function and increased mean arterial pressure are independent risk factors of PE in the second trimester pregnant women. There was a close positive correlation between serum ［Ca²⁺］_o and CD4⁺CD25⁺Treg inhibition rate.]]> 2017/12/18 8:27:00 MA Yin-yao, YAN Si-ping, TIAN Mao, et al. 476true ［Abstract］　Objective　To explore the diagnostic value of susceptibility weighted imaging(SWI) in patients with traumatic cerebral microbleeds.Methods　MRI data of 47 patients with traumatic cerebral microbleeds proved by clinical manifestation and operation were retrospectively analyzed. MRI data included T₂WI, T₁fluid attenuated inversion recovery(FLAIR), T₂FLAIR，DWI and SWI. The detection rates between SWI and conventional sequences were compared.Results　Forty-seven patients with traumatic cerebral microbleeds were showed punctate small patchy and linear low signal by SWI,and 14, 23, 12, 29 patients with traumatic cerebral microbleeds were showed by T₂WI，T₂FLAIR，T₁FLAIR，DWI respectively.Conclusion　SWI is sensitive to traumatic cerebral microbleeds and it has high value in early diagnosis of traumatic cerebral microbleeds.]]> 2017/12/18 8:27:00 HUANG Wen-liang, ZHOU Shan, LIU Xiu-min, et al. 475true ［Abstract］　Objective　To explore the application value of high-throughput sequencing technique(next generation sequencing, NGS) in prenatal diagnosis by using NGS to test fetal free DNA in maternal amniotic fluid.Methods　The amniotic fluid samples from 101 pregnant women were collected. Their gestational age was between 18 and 24 weeks and had high risks of Down syndrome and/or fetal abnormalities shown by color Doppler ultrasound. The amniotic fluid samples were drawn from the pregnant women and their amniotic fluid DNAs were extracted for preparing a sequencing library. By using Ion Proton, high-throughput sequencing procedure was carried out. The sequencing data were compared with the human reference gene-database and statistically analyzed. Simultaneously, the amniotic fluid was also collected for the chromosome karyotype analysis which compared with the NGS.Methods　By NGS, in 2 out of 101 cases showed chromosomal numerical abnormality, 37 cases of chromosome fragment deletion/duplication. By the standard of chromosomal karyotypic analysis, 2 cases showed chromosomal numerical abnormality, 2 cases showed chromosome 9 inversion, 2 cases showed chromosome polymorphism.Conclusion　The NGS tested for chromosomal numerical abnormality in the maternal amniotic fluid has the same sensitivity and specificity as the chromosome karyotype analysis and can detect micro-deletion/duplication. The combination of chromosome karyotype analysis and high throughput sequencing technology has good clinical application prospects in the detection of birth defects.]]> 2017/9/12 9:14:26 HE Bing, HUANG Li, ZHANG Peng, et al. 474true ［Abstract］　Objective　To evaluate the effect of hyperbaric oxygen on the coagulation function and blood cell changes in the treatment of rabbit renal injury induced by extracorporeal shock wave lithotripsy(ESWL).Methods　Twelve rabbits were randomly divided into the control group and the experimental group. The control group were given ESWL impact on the left idney for 1 500 times(capacitance 0.4 μF, voltage 18 kV) to cause kidney damage, but without hyperbaric oxygen treatment. The experimental group receival hyperbaric oxygen treatment 0.2 MPa(2.0 ATA) for continuous 5 days. The changes in blood white blood cell(WBC), red blood cell(RBC), platelet(PLT), prothrombin time(PT), and activated part clotting live enzyme time(APTT) were observed before the treatment and 1, 3, 7 days after the treatment respectively.Methods　There was no significant difference in RBC between the two groups after the hyperbaric oxygen treatment(HBOT)（P>0.05）. WBC, PT, APTT were significantly decreased in the experimental group at 1, 3 days after the HBOT compared with those in the control group, PLT were elevated in the experimental group（P<0.05）, and almost recovered back to their original levels 7 days after the HBOT(P>0.05).Conclusion　Hyperbaric oxygen reduces inflammation reaction, but does not induce or aggravate renal severe bleeding in the treatment of renal injury induced by ESWL. By increasing blood flow and oxygen supply to the important visceral organs.]]> 2017/9/12 9:14:26 LI Ji-qing, CHEN Fu-chang, LIU Gang, et al. 473true ［Abstract］　Objective　To observe the risk factors and prevention strategies for the treatment of early type 2 diabetic nephropathy.Methods　Seventy-three elderly patients with early type-2 diabetic nephropathy were taken as the research objects(DN group) and other matched 35 cases with diabetic but without nephropathy as the controls(DM group). The inflammation factors（hs-CRP, TNF-α, IL-6, IL-1β）, fasting blood-glucose（FBG）, 2-hour postprandial blood glucose（PBG）, glycosylated hemoglobin（HbA1c）, blood lipid levels（TC, TG, HDL, LDL）, Uric acid (UA), fasting insulin(FINS) and the insulin resistance index(HOMA-IR) were detected. Urinary albumin excretion rate(UAER) was tested in DN group. The data of height, weight and blood pressure were also collected.Methods　The levels of inflammation factors hs-CRP, TNF-α, IL-6, IL-1β and LDL, UA, HbA1c, FINS and HOMA-IR were significantly more elevated in DN group than those in DM group（P<0.05）. The levels of UAER were positively related to TNF-α, IL-6 and HOMA-IR（r=0.549, 0.584, 0.894, P<0.01）.Conclusion　UAER is associated with the levels of inflammatory and HOMA-IR in the elderly patients with early type-2 diabetic nephropathy.]]> 2017/9/12 9:14:26 LI Jian-ying 472true ［Abstract］　Objective　To evaluate the clinical efficacy of percutaneous vertebroplasty(PVP) for the patients with osteoporotie vertebral compression fractures(OVCF).Methods　From June 2013 to November 2014, 28 patients with OVCF were assigned to receive PVP. Of the 28 cases, there were 8 with thoracic vertebrae and 20 with lumbar vertebra. All the patients were followed up. The nerve functional restoration at the last follow-up was evaluated by ODI scores, and the height of vertebral body and the angle of Cobb were measured by accumulating the X-ray films of spine for measuring.Methods　28 patients were followed up for an average of 15.8 months(range from 12 to 23 months). VAS scores ascended from preoperative (8.7±3.5) to postoperative (1.9±2.2)(P<0.01). ODI scores ascended from preoperative (68.4±13.3)% to postoperative (10.1±6.6)%(P<0.01). The height of vertebral body ascended from preoperative (58.1±15.4)% to postoperative (62.2±8.6)%(P>0.05). Cobb of cervical spine ascended from preoperative (21.3±3.8) to postoperative (18.2±2.5)(P>0.05).Conclusion　PVP can significantly reduce the pain and improve the quality of life, showing the preferable clinical benefits, which appears to be a useful and effective manner for the treatment of OVCF.]]> 2017/9/12 9:14:26 GAN Xin-rong, RAO Min-jie, ZHANG Gong-heng, et al. 471true ［Abstract］　Objective　To explore the clinical value of single-port laparoscopic total hysterectomy(LESS-TH).Methods　One hundred and twenty cases with non-gynaecological malignant diseases were randomly divided into the experimental group(n=60) and the control group(n=60). The experimental group received single-port LESS-TH and the control group received traditional LESS-TH. The operation time, the bleeding volume, the hospitalization time, the postoperative morbidity, the postoperative anal exhaust time and the patients′ satisfaction were compared between the two groups.Methods　There were no significant differences in the time of surgery, intraoperative hemorrhage volume and the hospitalization time between the two groups(P>0.05). The postoperative pain score, the postoperative anal exhaust time and the patients′ satisfaction were significantly improved in the experimental group than those in the control group(P<0.05). The operation was successful in both of the two groups without postoperative complications, dead cases or failed cases.Conclusion　Laparoendoscopic single-port surgery is a safe and feasible operation mode for hysterectomy, with a reduction of postoperative pain, faster recovery, and a better cosmetic result.]]> 2017/9/12 9:14:26 HUANG Hui-yuan, ZHAO Ren-feng, LI Xue, et al. 470true ［Abstract］　Objective　To analyze the electrogastroenterogram data in the patients with acute appendicitis and to understand its gastrointestinal electric activity.Methods　Thirty-two cases with acute appendicitis were checked for eletrogastroenterogram and the average amplitude(P), average percentage frequency(f), gastric electric rhythm disorders(RD), reaction area(RA), percentage of normal slow wave(PSW) of the gastric electrointestinogram waveform were observed.Methods　In 1, 2, 3, 4-leads electrogastroenterogram,the average waveform amplitude(P) was in （183.44±100.98）～（212.44±109.9）, the average waveform frequency(f) in （3.33±0.64）～（3.47±0.74）, the gastric electric rhythm disorders percentage(RD) in （26.16±6.88）～（27.8±7.23）, the waveform reaction area(RA) in （67.84±35.55）～（73.00±40.51）;and the percentage of normal slow wave(PSW) in （50.84±8.59）～（52.86±7.76）; In 5, 6, 7, 8-leads electrogastroenterogram,P was in （127.06±73.97）～（135.00±77.74）, f in （11.18±3.07）～（12.35±5.12）, RD in （26.66±4.85）～（28.06±5.23）, RA in （46.16±27.21）～（50.16±29.75）, and PSW in （45.05±7.35）～（47.68±7.06）.Conclusion　The gastrointestinal electric rhythm in the patients with acute appendicitis is in a state of disorder.]]> 2017/9/12 9:14:26 ZHANG Liang-qing, FENG Shi-bin, GAO Hai-hong, et al. 469true ［Abstract］　Objective　To investigate the application value of dexmedetomidine(DEX) in acute coronary syndrome(ACS) and PCI operation.Methods　Ninety-nine patients with ACS and PCI operation in our hospital from September 2008 to June 2012 were randomly divided into DEX group（n=33）, propofol group （n=33） and control group（n=33）. The control group received the therapy of saline infusion and the propofol group received conventional anesthesia induction therapy of propofol(1～2 mg/kg), and the DEX group received anesthesia induction therapy of DEX(0.5 g/kg). The levels of plasma epinephrine(E) and norepinephrine(NE) in the three groups were detected by high-efficiency chromatographic analysis method, and the levels of plasma thromboxane B2(TXB2), and 6-ketone-alpha prostaglandin F1(6-Keto-PGF1α) were detected by radioimmunity method. Visual analogue scale(VAS) was used for pain evaluation. Heart rate(HR), mean arterial pressure(MAP), VAS and the levels of E, NE, TXB2, and 6-Keto-PGF1α in plasma in the three groups were statistically analyzed before and after the surgery.Methods　T₂, T₃ and T₄ HR in the DEX group after the DEX injection were significantly lower than those in the propofol group; T₂, T₃ and T₄ HR in the propofol group were significantly lower than those in the control group(P<0.05). T₂, T₃ and T₄ MAP in the DEX group were significantly lower than those in the propofol group; T₂, T₃ and T₄ MAP in the propofol group were significantly lower than those in the control group(P<0.05). The levels of plasma E, NE and TXB2 in the DEX group after DEX injection were significantly lower than those in the propofol group; The levels of plasma E, NE and TXB2 in the propofol group were significantly lower than those in the control group; The level of plasma 6-Keto-PGF1α of the DEX group was higher than that of the propofol group while its VAS scores were lower than those of the propofol group; The level of plasma 6-Keto-PGF1α of the propofol group was higher than that of the control group while its VAS scores were lower than those of the control group(P<0.05).Conclusion　Using DEX in anesthesia induction is beneficial to maintaining the dynamic balance of perioperative blood flow, inhibiting the body′s stress response to the perioperative blood and relieving the pain in patients with ACS during PCI.]]> 2017/9/12 9:14:26 LI Yu-zhong, DENG Yan-na, LONG Hong-jie 468true ［Abstract］　Objective　To elucidate the serum levels of complement C1q and their correlation with other laboratory parameters in HIV/AIDS patients prior- and post-treatment.Methods　A retrospective study was performed in which 96 HIV/AIDS patients were included from September 2011 to October 2014 in the Fourth People′s Hospital of Nanning in Guangxi Zhuang Autonomous Region and were treated with antiretroviral therapy. The serum complement C1q and other laboratory parameters in the patients were detected and analyzed before and 6 months after the treatment.Methods　The concentration of serum complement C1q was significantly reduced in HIV/AIDS patients after antiretroviral therapy(P<0.05). Among them, the serum C1q level was elevated in 33 cases after 6 months of the treatment comparing to 63 cases who showed serum C1q reduction(P<0.05). Blood CD3, CD4, CD8 counts, and CD4/CD8 ratio were elevated while the viral load testing results showed decrease after the treatment comparing with those before the treatment(P<0.01).Conclusion　Combination analysis of serum C1q level, T lymphocyte subsets, and viral load is a new and useful clinical laboratory diagnostic strategy and should be considered as a routine monitoring parameter set in HIV/AIDS treatment and prognosis.]]> 2017/9/12 9:14:26 LUO Xiao-lu, SU Guo-sheng, WEI Li-bin, et al. 467true ［Abstract］　Objective　To establish a hypertension rat model induced by chronic intermittent hypoxia, and to provide a simple and duplicate animal model for the study of the pathogenesis of hypertension in OSAHS.Methods　The intermittent hypoxia system was used to conduct the experiment, including the control subsystem, the gas supply subsystem and the exposed cabin. 24 male Sprague-Dawley rats, aged 8 to 10 weeks, and weighted from 200 to 250 g were randomly divided into two groups: the intermittent hypoxia intervention group and the control group. The intermittent hypoxia intervention group(n=12) was exposed to 6% to 8% oxygen for 40 seconds and then to 21 % oxygen for 80 seconds in the exposed cabin for 8 hours per day with many intermittent hypoxia cycles. The control group(n=12) was exposed to certain concentration oxygen chamber. The tail artery systolic pressure of the rats was taken at rest states for three consecutive times and the average was calculated.Results　The intermittent hypoxia intervention group had a significantly higher systolic blood pressure than the control group at the 35th day of the experiment［（136.21±32.46）mmHg vs (121.37±35.37）mmHg, P<0.05］.Conclusion　Precisely aerating the oxygen on and off in an certain range of concentration can effectively make a simulated intermittent hypoxia process, which is an ideal animal model for studying the mechanisms of intermittent hypoxia-induced hypertension.]]> 2017/9/13 9:54:48 JIANG Li-jun, LIU Jian-hong, SONG Wei, et al. 466true ［Abstract］　Objective　To investigate the value of contrast-enhanced ultrasound(CEUS) and contrast-enhanced CT(CECT) in the diagnosis of small liver cancer.Methods　One hundred and tweleve patients with 143 small solid liver lesions (≤2.0 cm) were examined with contrast-enhanced ultrasound and contrast-enhanced CT, and the diagnoses were confirmed by pathological examination. CEUS and CECT findings of 143 small solid liver lesions in the patients were retrospectively analyzed.Results　111 malignant lesions including 79 primary liver carcinoma, 8 intrahepatic cholangiocarcinoma and 24 metastatic liver carcinoma and 32 benign lesions were found in 112 patients with 143 lesions. Compared with the results of pathological diagnosis, the sensitivity, specificity and accuracy of CEUS and CECT were 88.3%, 84.4%, 87.3% and 86.5%, 87.5%, 86.7%, respectively, without significant differences between CEUS and CECT in the diagnosis （P>0.05）.Conclusion　CEUS and CECT have similar effects in the diagnosis of small liver carcinoma(≤2.0 cm). CEUS is simpler than CECT in the diagnosis of small liver carcinoma.]]> 2017/9/13 9:54:48 XU Chun-mei, LIU Tian-qi, MO Xiang-lan, et al. 465true ［Abstract］　Objective　To evaluate the clinical significance of urinary antigen detection for rapid diagnosis in patients with community-acquired pneumonia and to understand the practical value in basic level hospitals.Methods　One hundred and two adults with community-acquired pneumonia were enrolled in this study from March 2014 to April 2015 in three hospitals. Their serum and urine were collected and the pneumococcal antigens in the urine were detected using the Binax immmunochromatographic test(ICT). Bacteria were cultured from sputum.Results　Among 102 cases with community-acquired pneumonia, the urine ICT was positive in 15.69%(16/102). Sputum culture was positive in 2.99%(2/67). The total positive rate of pathogens was 19.61%（20/102）.Conclusion　Urinary antigen detection provides a credible rapid diagnostic test for pneumococcal pneumonia. It is suitable for applying in basic level hospitals.]]> 2017/9/13 9:54:48 AN Dong-shan, SUN Wei-hong, ZHU Long-you, et al. 464true ［Abstract］　Objective　To explore the changes of echocardiography on the patients with left breast cancer who received intensity modulated radiation therapy(IMRT).Methods　Eighteen patients with left breast cancer were performed by echocardiography before and one month after IMRT. The cardiac morphology, hemodynamic and cardiac function were compared before and after the treatment.Results　The detection value of cardiac ultrasound had no significant difference before and after IMRT(P>0.05); Mitral valve had no significant difference between systolic and diastolic peak velocity ratio(P>0.05); Cardiac function indexes of EF, FS and ESV showed significantly differences before and after the treatment(P<0.05).Conclusion　There are some changes in cardiac function indexes in the patients with left breast cancer after IMRT. Echocardiography is of great value to the evaluation of cardiac morphology, hemodynamics and cardiac in the patients with left breast cancer after IMRT.]]> 2017/9/13 9:54:48 WAN Li-kai, ZHANG Qin, ZHANG Ya-li, et al. 463true ［Abstract］　Objective　To investigate the frequencies of the mitochondrial tRNA Leu（UUR） mutations at position 3243A/G in newborns of diabetic pregnant women in Guangxi.Methods　The point mutations of tRNA Leu（UUR）3243A/G in 50 newborns of diabetic pregnant women and 50 newborns healthy controls were detected by DNA sequencing.Results　No one carrying the mitochondrial tRNA Leu（UUR）3243A/G mutation was found in the two groups.Conclusion　The mitochondrial tRNA Leu（UUR）3243A/G mutation may not yet be an important genetic predisposing factor of prenatal screening for gestational diabetes mellitus in Guangxi.]]> 2017/9/13 9:54:48 LI Ying, WANG Lin, QIN Ting, et al. 462true ［Abstract］　Objective　To evaluate the efficacy of potassium sodium hydrogen citrate on residual stone removal and prevention of stone recurrence after percutaneous nephrolithotomy(PCNL) for renal calculi.Methods　One hundred and fifty-four patients with residual stones or without residual stones after PCNL were enrolled in this study. Patients without residual stones(n=84) or with residual stones(n=70) were randomly divided into the treatment group and the control group respectively. The treatment group was given orally potassium sodium hydrogen citrate granules 10 g per day, and the control group was not treated with any medication. The stone recurrence was compared between the two groups after a follow-up of 12 months.Results　For patients without residual stones, the stone recurrence rate in the treatment group was significantly lower than that in the control group(8.33% vs 38.46%, P<0.05). For patients with residual stones group, the stone growth rate in the treatment group was also significantly lower than that in the control group(10.71% vs 59.37%, P<0.05).Conclusion　Potassium sodium hydrogen citrate therapy can not only reduce the stone recurrence, but also inhibit the residual stone growth after PCNL.]]> 2017/9/13 9:54:48 LAN Zhi-xiang, WANG Wei, CAI Bin, et al. 461true ［Abstract］　Objective　To study the relationship between cognitive function and plasma homocysteine(Hcy) level in vascular dementia(VaD) patients.Methods　Forty-six hospitalized VaD patients were collected as VaD group and other 50 patients with non-demented cerebral infarction were collected as cerebral infarction group from June 2013 to February 2015. 40 healthy elderly patients were taken as the control group. The severity of cognition impairment of VaD patients was classified by MMSE′s scores. The levels of plasma Hcy, folic acid and vitamin B₁₂ were detected in all these patients, and the relationships between these indicators and the severity of cognition impairment were studied.Results　The levels of plasma Hcy in VaD group and cerebral infarction group were higher than those in the control group(P<0.05). The levels of folic acid and vitamin B₁₂ in VaD group and cerebral infarction group were lower than those in the control group(P<0.05). The level of plasma Hcy in VaD group was higher than that in cerebral infarction group(P<0.05), while the levels of folic acid and vitamin B₁₂ in VaD group were lower than those in cerebral infarction group(P<0.05), the concentrations of plasma Hcy increased, folate and vitamin B₁₂ decreased significantly in VaD patients with the increasing severities of cognition impairment(P<0.05).Conclusion　The level of plasma Hcy is associated with the occurrence and development of VaD. Hyperhomocysteinemia may be a risk factor for VaD. The detection of Hcy level is beneficial to the prevention and cure of VaD.]]> 2017/9/13 9:54:48 LIU Shi-mei, WU Xiao-hui, CAI Si-min, et al. 460true ［Abstract］　Objective　To observe the therapeutic effects of two kinds of maintenance therapy on treating recurrent vulvovaginal candidiasis(RVVC) and to find a maintenance therapic method with shorter course of treatment and better curative effect.Methods　One hundred and twelve patients with RVVC were randomly divided into the observation group(n=56) and the control group(n=56). The observation group was simply given vitamin C tablets in vagina during maintenance therapy for three months; The control group was treated with oral fluconazol and vaginal suppository of clotrimazole for six months. The clinical efficacy, adverse reactions and recurrence rate were compared between the two groups.Results　The total effective rates were 96.4% and 98.2% in the observation group and the control group respectively(P>0.05); Vaginal pain occured in five patients in the observation group(8.9%), while more adverse reactions including nausea, vomiting and vaginal burning sensation occur in thirteen patients in the control group(23.2%), with a significant difference in adverse reaction rate between the two groups(P<0.05). The disease reoccurred in two cases(3.6%) in the observation group one year after the treatment, while three cases(5.4%) in the control group, without a significant difference between the two groups(P>0.05).Conclusion　External use of vitamin C tablets in vagina has some advantages of a shorter course, satisfactory curative effect and less drug adverse reactions for the treatment of RVVC.]]> 2017/9/13 9:54:48 LI Bao-zhen, FAN Xiao-ling, DENG Chun-rong, et al. 459true ［Abstract］　Objective　To observe the roles of glargine on colon cancer LoVo cells in vitro and to investigate the potential mechanisms.Methods　Colon cancer LoVo cells were treated with different concentrations of glargine and human insulin. Proliferation of the cells treated with glargine was assayed by CCK8. Apoptosis in the treated cells was measured by flow cytometry. The protein expression of ERK1/2 in treated cells was determined by Western blot.Results　Glargine(20,50,100,150,200 nM) dramatically increased the LoVo cells number.Compared with the control, both glargine(100,150,200 nM) and human insulin(100 nM) as the positive control induced significant cells proliferation(P<0.05). The rate of cell early apoptosis in the control was 13.26%, while the rate decreased to 11.52%, 9.11% and 6.08% after the cells treated with glargine at 100, 150 and 200 nM, respectively. After ERK1/2 inhibitor PD98059 was added into LoVo cells, proliferation was not induced by glargine and early apoptosis rate was not innduced. An increase in glargine(200 nM) stimulated p-ERK1/2 occurred in a time and dose dependent manner. However, the total protein levels of ERK1/2 remained unaffected throughout the course of these experiments.Conclusion　Glargine can induce the proliferation of colon cancer LoVo cells in vitro. ERK1/2 signal pathway activation may be the potential mechanism.]]> 2017/9/15 8:39:15 TENG Jia-an, QIN Jian 458true ［Abstract］　Objective　To study the therapeutic effects of HuzhangJiangzhi Granules on hyperlipidemia in diet-induced rats.Methods　The hyperlipidemia rat model was induced by administrating with high fat emulsion and different doses of HuzhangJiangzhi Granules were given orally to rats. The effects of the drug on the levels of lipids, AST, ALT, creatinine, urea nitrogen in serum, the levels of TC, TG in liver, the liver index and morphologic changes of liver were observed.Results　HuzhangJiangzhi Granules in different doses reduced significantly the levels of liver index, liver TC and TG, serum TC, LDL-C and HDL-C, serum AST and ALT（P<0.05 or P<0.01）, but had no effects on serum TG levels, serum creatinine and urea nitrogen. The results of liver histopathology indicated that HuzhangJiangzhi Granules reduced significantly liver steatosis.Conclusion　HuzhangJiangzhi Granules can regulate hyperlipidemia in rats.]]> 2017/9/15 8:39:15 GUO Mei-hong, HUANG Xiao-dan, FAN Ying, et al. 457true ［Abstract］　Objective　To evaluate the clinical results of the hip replacement for treating the unstable intertrochanteric fractures in the elderly patients.Methods　Thirty-eight elderly patients(aged 75 and above) with unstable intertrochanteric fractures were treated by the hip arthroplasty in our hospital from January 2004 to August 2009. All the patients were followed up to assess the effects.Results　All of the patients were followed up for (6.2±1.2)years, without dead cases during perioperation. The one-year mortality was 7.9% and the five-year mortality was 34.2%. The intraoperative blood loss was (444.7±304.3)ml in average and the mean time of postoperative activities was (4.7±3.4)d. Artificial joint dislocation happened in one case and prosthesis subsidence in one case when the patients were followed up for four years after the surgery. Acetabular erosion was found in one case over the five-years follow-up. The Harris scores were (77.8±7.5), (85.1±6.4) and (81.2±7.2) in average respectively after three months, one year and five years of the operation and the excellent and good rates were respectively 63.2%, 85.7% and 80.0%.Conclusion　The hip replacement for the unstable intertrochanteric fractures in the elderly patients can shorten the time in bed and reduce the complications. It may benefit the patients with earlier function recovery, and keep long-term of good curative effect.]]> 2017/9/15 8:39:15 HUANG Xiao, YIN Dong, MO Bing-feng, et al. 456true ［Abstract］　Objective　To investigate the changes in the size and shape of the levator hiatus in postpartum women with different delivery methods by transperineal three-dimensional ultrasound(3D).Methods　Sixty-five postpartum women after 42～45 days postpartum between December 2013 and December 2014 were included in this study, with 30 cases in the cesarean group and 35 cases in the normal delivery group. 25 cases of nulliparous women were included in the control group. The images of rest phase and Valsalva phase were collected. The changes in the size and shape of the levator hiatus of the two phases were observed, and the morphological parameters were measured.Results　In the rest phase and Valsalva phase, the transversal diameter, anteroposterior diameter and areas in both of the cesarean group and the normal delivery group were significantly greater than those in the control group. The thicknesses of the bilateral puborectalis of the above two groups were significantly smaller than those of the control group（P<0.05）. The transversal diameter, anteroposterior diameter and area of the normal delivery group were greater than those of the cesarean group, and the thickness of the bilateral puborectalis of the normal delivery group was significantly smaller than that of the cesarean group（P<0.05）.Conclusion　Levator hiatus becomes bigger and lavatory ani muscle becomes thinner in a postpartum woman than those in a nulliparous woman. Levator hiatus becomes bigger and lavatory ani muscle becomes thinner in a normal delivery woman than those in a cesarean woman. Transperineal 3D ultrasound is an effective imaging method for showing levator hiatus in postpartum women.]]> 2017/9/15 8:39:15 NONG Mei-fen, LING Bing, CHEN Hai-ning, et al. 455true ［Abstract］　Objective　To investigate the cervical ripening effect of double lumen Foley catheter in obstetrical delayed pregnancy.Methods　Two hundred pregnant women who had delayed pregnancy were randomly divided into the experimental group(n=100) and the control group(n=100). On the day of admission the control group was given the low concentration of oxytocin(2.5 U in 500 ml normal saline) intravenously for labor induction. On the next day after admission, the experimental group received routine disinfection for vulva, vagina and cervix,then the double lumen Foley catheter was inserted into the cervix. On the next day after catheterization, cervix was examined for cervical score, with cervical scores more than seven points or more being defined as effectiveness. For pregrant women with irregularuterine contraction, the low contraction of oxytocin was given(the dose was the same as that in the control group). The infusing speed was adjusted according to the condition of uterine contraction and the condition that timely artificial rupture was performed; Pregnant women who had cervix dilativn of 3 cm and regular contractions should be expected natural childbirth. Cervical score, the onset time of labor and total delivery labor time, neonatal scoring after birth, postpartum morbidity(reproductive tract infections and cervical laceration)were observed in the two groups, and the clinical effects after treatment were compared between the two groups.Results　The effective rate in the experimental group was significantly higher than that in the control group(P<0.05). The cervical score in the experimental group was significantly higher than that in the control group after catheterization for 12 hours(P<0.05). The cesarean section rate was significantly lower in the experimental group than that in the control group(14.00% vs 29.00%)(P<0.05). The onset time of labor and the total delivery labor time in the experimental group were significantly shorter than those in the control group(P<0.01). There were no significant differences in the incidence of postpartum(reproductive tract infections and cervical laceration), birth weight, neonatal scoring between the two groups(P>0.05).Conclusion　Application of double lumen Foley catheter for cervical ripening in delay pregnancy is safe and effective.]]> 2017/9/15 8:39:15 LIANG Qing-fen 454true ［Abstract］　Objective　To investigate the clinical effect of modified sacral injection of triple drugs in the treatment of senilelumbar disc herniation.Methods　Eighty patients with lower lumbarprotrusion of the discogenic pain were collected in the inpatients and outpatients in our hospital from November 2011 to June 2014 and divided into the treatment group(n=42) and the control group(n=42). The treatment group was given lidocaine 5 ml and dexamethasone 5 mg, vitamin B₁₂ 0.5 mg, 42～45 ℃ 20 ml of physiological saline for sacral canal injection, followed by sacral canal injection with lidocaine 5 ml, dexamethasone 5 mg, 42～45 ℃ 10 ml of physiological saline, 1 time every three days, three times as a period of treatment. The control group was given massage therapy, 1 time per day,10 times as a period of treatment.Results　There were significant differences in VAS scores between the two groups at different time points(F_time-point=9.76,P_time-point=0.00), and the lowest scores occurred on the tenth day after the treatment in both of the two groups. The VAS score of the treatment group was significantly lower than that of the control group(F_{between-group}=5.63, P_{between-group}=0.01). There were interactions between the different groups and the different time points(F_{group category×point}=5.44, P_{group category×point}=0.01). 26 cases were cured, 14 cases improved and 2 cases invalid in the treatment group, and 15 cases were cured, 20 cases improved and 7 cases invalid in the control group. The clinical curative effect of the treatment group is better than that of the control group(Z=-2.574, P=0.010).Conclusion　Sacral canal injection of triple-drug therapy for elderly patients with lumbar disc herniation is safe and effective. It is suitabale for outpatient treatment.]]> 2017/9/15 8:39:15 SONG Zhi-jing, WU Jian-min, LI Ning, et al. 453true ［Abstract］　Objective　To evaluate the clinical value of the ratio of the alpha fetoprotein(AFP) and standard remnant liver volume in the recurrence risk of primary liver cancer after resection.Methods　Sixty-eight patients′ clinical data were retrospectively analyzed, who were given the primary liver cancer resection in our hospital from May 2009 to September 2014, with a follow-up of 1 year. They were divided into two groups according to whether the disease reoccurred or not. The recurrence group contained 32 patients, and the non-recurrence group contained 36 patients. The mean AFP and standard postoperative residual liver volume were compared between the two groups to evaluate the clinical value of the ratio of the alpha fetoprotein(AFP) and standard remnant liver volume in the recurrence risk of primary liver cancer after the resection.Results　The postoperative AFP was (110.25±89.44)ng/ml and (70.71±54.32)ng/ml respectively in the recurrence group and the non-recurrence group; The standard remnant liver volume was (371.59±27.35)ml/m² and (462.56±61.96)ml/m² respectively in the recurrence group and the non-recurrence group. The ratio of AFP to standard remnant liver volume of the recurrence group was (0.29±0.22), which was significantly higher than that of the non-recurrence group(0.16±0.16)(P<0.01).Conclusion　There is certain clinical value of the ratio of AFP to standard remnant liver volume in the recurrence risk of primary liver cancer after resection. When it exceeds 0.2, the recurrence risk of primary carcinoma of the liver after resection will be higher in 1 year.]]> 2017/9/15 8:39:15 HU Hua-hua, XU Jie-hong, YI Hong, et al. 452true ［Abstract］　Objective　To evaluate the diagnostic value of multipie slice spiral CTA of hepatic vessels in the patients with liver cirrhosis.Methods　Tri-phase enhanced CT scans of whole hepatic region were performed respectively in 138 patients with liver cirrhosis(the liver cirrhosis group) and in 50 patients without liver cirrhosis(the control group). The images of hepatic arteries, splenic arteriea and its branches were post-processed with MIP, MPR and VR, and their images were compared between the two groups.Results　There were significant differences between the two groups in showing hepatic artery,portal vein and its branches(P<0.01). There were no statistical differences in showing portal vein and Child grade system(P>0.05). CTPV images displayed the portal venous system and its collateral circulation stereoscopically. The vascular assessments were performed in 27 patients with hypersplenism and in 23 patients with liver cancer using CTA before interventional therapy.Conclusion　The changes of hepatic arteries and splenic arteriea may indicate the severity of hepatic cirrhosis, and provide the reliable evidences for imaging diagnosis of the disease.]]> 2017/9/15 8:39:15 LI Rui-xiong, YANG Wei-zhen, JIANG Shi-wu 451true ［Abstract］　Objective　To explore the clinical effects of medical staff guided-home massage on the community children with anorexia of spleen-stomach qi deficiency type.Methods　Sixty cases were randomly divided into the massage group 1(n=30) and the massage group 2(n=30). Besides the behavioral intervention and dietary guidance, the children in the massage group 1 received acupoint selected massage by community medical staff; the children in the massage group 2 received massage by parents under the guidance of community medical staff. All patients were treated for 8 weeks. The clinical symptom scores and clinical efficiency were compared before and after the treatment in both of the two groups and their adverse reactions were recorded.Results　After the treatment, the clinical symptom scores of appetite, colour of face, mental weariness, halitosis, abdominal distension and stools in the two groups decreased greatly(P<0.05). In the massage group 1, 12 cases were cured, 8 markedly effective, 9 effective and 1 ineffective; in the massage group 2, 9 cases were cured, 6 markedly effective, 13 effective and 2 ineffective. There were no significant differences between the two groups(P>0.05).Conclusion　Massage is a safe, effective, convenient and simple treatment for the children with anorexia. The medical staff guided-home massage has the similar effect.]]> 2017/9/15 8:39:15 LUO Ting 450true ［Abstract］　Objective　To explore the relationship between the kinetic parameters and the developmental potential of embryos, and to provide the evidences for the selection of better developmental potential embryos.Methods　A retrospective analysis of 165 2PN embryos was performed in our center using time-lapse technology. The embryos were divided into different groups according to the criteria of the embryo scores and the kinetic parameters were compared among the groups.Results　（1）The embryos were divided into two groups according D3 embryo scores: grade 1～2 group(n=85) and grade 3～4 group(n=80). The development of the grade 3～4 group in the parameters of tPNa, tPNf and t2 was delayed than that of the grade 1～2 group, but without a significant difference between the two groups(P>0.05). The time of t3/t4 in the grade 3～4 group was less than that in the grade 1～2 group, but without a significant difference(P>0.05). （2）The embryos were divided into three groups according D5 embryo scores: high quality blastocysts group(n=20), frozen blastocysts group(n=17) and poor quality blastocysts group(n=23). The parameters of tPNa and t2 in the frozen blastocyst group were slightly delayed than those in the high quality blastocysts group(P>0.05). The time of tPNa and t2 was significantly delayed in the poor quality blastocysts group than that in the high quality blastocysts group(P<0.05). There were significant differences among the three groups in the parameters of tSC, tMf/p and tSB(P<0.05).Conclusion　The time parameters of t2, tSC, tMf/p and tSB may be related to the formation of the blastocyst, which can be used to predict the developmental potential of the embryos.]]> 2017/7/13 10:28:25 WANG Shi-kai, XUE Lin-tao, HE Bing, et al. 449true ［Abstract］　Objective　To summarize the thrombolytic therapy for the patients with acute ST-segment elevation myocardial infarction(STEMI) in a primary hospital.Methods　Ninety patients with STEMI in our hospital from November 2013 to November 2015 were randomly divided into two groups:group A(n=45) and group B(n=45). Group A was treated with reteplase and group B received urokinase. The clinical effect and the incidence of adverse reactions were observed and compared between the two groups.Results　Compared with group B, group A had a higher revascularization rate two hours(88.9% vs 71.1%, P<0.05), or six hours(66.7% vs 12.5%, P<0.05) after the thrombolytic therapy. Compared with group B, group A had a higher ejection fration, a lower BNP level and better cardiac function(P<0.05). There were no significant differences in the risk of bleeding and death between the two groups.Conclusion　Reteplase can recanalize the infarct-ralated arteries, reduce myocardial injury, improve the heart function and prognosis in the patients with early STEMI.]]> 2017/7/13 10:28:25 XIE Wen-tao, ZHANG Zhi-yang, YAN Wen-mei, et al. 448true ［Abstract］　Objective　To explore the effect of highly active antiretroviral therapy on blocking mother-to-infant transmission of HIV in women with different weeks of pregnancy(HAART).Methods　Sixty pregnant women infected with HIV were divided into the 14-week-pregnant group(n=30) and the 28-week-pregnant group(n=30). Both of the two groups received AZT+3TC+LPV/r from January 2012 to January 2014 to block mother-to-infant transmission of HIV. The changes of CD4⁺ T lymphocytes and virus load were tested before the treatment and one month before due dates. The growth and development of the babies at birth were observed. and their HIV antibodies were detected when they were 18 months old.Results　The CD4⁺ T lymphocytes were significantly higher and the virus load was lower in the 14-week-pregnant women than those in the 28-week-pregnant women before the treatment and one month before their due dates(P<0.05). There were no significant differences between the two groups in the conditions of the babies at birth including premature delivery, low birth weight, suffocating, and the positive rate of HIV antibody(P>0.05).Conclusion　A similar outcome is showed in the babies of 14-week-pregnant women and 28-week-pregnant women, however less replication of virus and lower virus load are present in the babies of 14-week-pregnant women.]]> 2017/7/13 10:28:25 LIU Dong-mei, QIN Yan, WEI Dan-yan, et al. 447true ［Abstract］　Objective　To study thyroid peroxidase antibody(TPOAb) in the patients with Hashimotos Thyroiditis hypothyroidism and high uptake of thyroidal technetium-99m pertechnetate(^99mTc).Methods　 The serum free T3, free T4, sTSH, TPOAb and thyroid uptake of ¹³¹I and ^99mTc were dectected in 60 patients with Hashimotos Thyroiditis hypothyroidism in whom 30 patients with high ^99mTc uptake were taken as the study group, and the other 30 patients with normal or low ^99mTc uptake as the control group.Results　The uptakes of TPOAb, sTSH and thyroidal ^99mTc were significantly higher in the study group than those in the control group（P<0.05）; There was a positive correlation between TPOAb and thyroidal ^99mTc uptake（r=0.381, P<0.05）.Conclusion　TPOAb is significantly higher in the patients with HT hypothyroidism and high thyroidal ^99mTc uptake. The more TPOAb, the higher thyroidal^99mTc uptake. These results indicate that TPOAb is one of the mechanism of high thyroidal ^99mTc uptake in the patients with HT hypothyroidism.]]> 2017/7/13 10:28:25 LIAO Ke-hua, LI Ni, LU Gui-nan, et al. 446true ［Abstract］　Objective　To explore the clinical effect of low dose artery chemoembolization on primary liver cancer.Methods　One hundred and fifty-nine patients with advanced primary liver cancer from December 2003 to August 2014 were randomly divided into two groups: group A(n=94) and group B(n=65). Group A was treated with low dose artery chemoembolization and group B was treated with high dose artery chemoembolization. The life quality, clinical response, tumor markers(AFP), tumor imaging(CT or MRI) and liver function changes were observed.Results　There was no significant difference in the relief rate of clinical symptoms between group A(76.5%) and group B(63.0%) after the treatment(P=0.065). The tumor shrinking rate was 25.5%(24/94) in group A and 29.2%(19/65) in group B six months after the treatment(χ²=0.266, P=0.605). The positive cases of preoperative AFP were 68 in group A and 46 in group B. The descent rate more than 50.0% was 55.8%(38/68) in group A and 52.2%(24/46) in group B one month after the treatment(χ²=0.152, P=0.695). The survival rate was 82.9%(78/94) in group A and 75.4%(49/65) in group B(χ²=1.378, P=0.240). The postoperative liver function of group A was significant better than that of group B 1, 3 and 5 days after the treatment.Conclusion　Low dose and high dose artery chemoembolizations are similarly effective in the treatment of primary liver cancer, but low dose artery chemoembolization has less adverse reactions on liver function.]]> 2017/7/13 10:28:25 JIN Xiao-jian, LU Bang-yu, CAI Xiao-yong, et al. 445true ［Abstract］　Objective　To explore the sedative effects and safety of different doses of fentanyl in combination with midazolam on the applicanion of fiberoptic bronchoscopy.Methods　Sixty patients undergoing fiberoptic bronchoscopy were randomly divided into two groups, with 30 cases in each group. On the basis of the local anesthesis of lidocaine was given,group A was given fentanyl 0.2 μg/kg combined with midazolam 0.05 mg/kg intravenously for sedation, and group B was given fentanyl 0.5 μg/kg combined with midazolam 0.1 mg/kg intravenously for sedation. The changes of heart rate(HR), diastolic blood pressure(SBP), systolic blood pressure(DBP), blood oxygen saturation(SpO₂) and recovery time and adverse reactions were compared between the two groups before the treatment and 1, 3, 5 and 10 minutes after the administration.Results　After the treatment at each time point, there were no significant differences in HR, SBP and DBP between the two groups(P>0.05). SpO₂ decreased in both of the two groups 3 min after the anesthesia, but it decreased more significantly in group B than in group A(P<0.05 ). The recovery time, incidence of adverse reactions and the awareness rate of anesthesia in group A were significantly lower than those in group B(P<0.05).Conclusion　Fentanyl 0.2 μg/kg in combination with midazolam 0.05 mg/kg has a good sedative effect and a little effect on oxygen saturation in the patients receiving inspection of fiber bronchoscope.]]> 2017/7/13 10:28:25 CHEN Run-sen 444true ［Abstract］　Objective　To explore the effect of mifepristone on the primiparas and neonates after the failure of emergency contraception.Methods　One hundred primiparas who took mifepristone for emergency contraception but failed were collected as the study group, and other 100 primiparas without the history of taking abortifacient pills as the control group. The inspections for fetal growth and fetal malformation were conducted by ultrasound at 13～15 weeks, 24 weeks of gestation and after delivery respectively, and the results were compared between the two groups.Results　The spontaneous miscarriage rate of the study group（13.0%） was significantly lower than that of the control group（1.0%）, P<0.01. There were no significant differences in fetal malformation and neonatal malformations between the study group and the control group(3.0% vs 2.0% and 3.0% vs 2.0%), P>0.05. The rate of neonatal asphyxia in the study group was significantly higher than that in the control group, P<0.05.Conclusion　The pregnant women taking mifepristone for emergency contraception but failing to abort the fetus have higher spontaneous abortion rate and neonatal asphyxia rate than the normal pregnant women.]]> 2017/7/13 10:28:25 LI Jing- ling, ZHAO Ren-feng 443true ［Abstract］　Objective　To investigate the guiding role on the assessment of asthmatic status and the guidance of treatment by measuring the fractional exhaled nitric oxide(FeNO) levels in children with asthma.Methods　A total of 87 asthmatic children aged 5～12 years from pediatric outpatient were studied, including 45 cases of mild asthmatic exacerbation group and 42 cases of moderate-severe exacerbation group. The FeNO levels of the children with asthma were measured by using SV-eNO-01 Nano Coulomb Nitric Oxide Detector and Sunvou-D100 Nano Coulomb expiration Analyzer respectively in acute asthmatic exacerbation and after the treatment for four weeks, and the questionnaire of asthma control test(ACT) was completed at the same time.Results　（1）The FeNO levels of asthmatic children (43.52±27.52)ppb were significantly higher before the treatment than those after the treatment (19.33±13.98)ppb and those of the control group (7.89±3.06)ppb (P<0.01). The levels of FeNO of asthmatic children after four weeks′ treatment were still significantly higher than those of the control group(P<0.01).（2）The levels of FeNO in the mild asthmatic exacerbation group (28.49±14.99)ppb were significantly higher than those in the control group before the treatment(P<0.01), and the levels of FeNO in the moderate-severe exacerbation group (59.97±29.37)ppb were significantly higher than those in the mild exacerbation group and the control group before the treatment(P<0.01).（3）There were no significant differences in FeNO levels between the mild asthmatic exacerbation group (13.77±7.19)ppb and the control group after the treatment(P>0.05), however, the FeNO levels in the moderate-severe exacerbation group (27.84±16.98)ppb were significantly lower than those before the treatment but significantly higher than those in the control group(P<0.01).(4)The variation of FeNO levels in the asthmatic children was positively correlated with ACT scores before(r=-0.706) and after the treatment(r=-0.566), P=0.000.Conclusion　FeNO levels are strongly related to the clinical features of childhood asthma. FeNO levels increase with the exacerbation of asthma and decrease after the standardized treatment.]]> 2017/7/13 10:28:25 XIE Qing-ling, ZHANG Di-wen, WANG Mei-chun, et al. 442true ［Abstract］　Objective　To investigate the outcomes of fiberbronchoscope treatment in patients with ventilator-associated pneumonia(VAP) after lung radical resection, and to provide the evidence for clinical treatment.Methods　Sixty patients with VAP in our hospital from December 2013 to December 2015 were collected and divided into the treatment group(n=30) and the control group(n=30) according to the different treating procedures. The treatment group received the fiberbronchoscope treatment and the control group received the routine endotracheal suction. pH, PaO₂, PaCO₂, SpO₂, the duration of mechanical ventilation, the time of chest X-ray improvement and the length of staying in the intensive care unit(ICU) were compared between the two groups before and after the treatment. SPSS19.0 software was used for statistical analysis.Results　There were no significant differences in pH, PaO₂, PaCO₂ and SpO₂ between the two groups before the treatment, however the results had a better improvement in the treatment group than those in the control group after the treatment(P<0.05). There were no significant differences in CPIS scores between the two groups, however the CPIS scores of the treatment group were significantly lower than those of the control group 1, 3 and 5 days after the treatment(P<0.05). The duration of mechanical ventilation, the time of chest X-ray improvement, and the length of staying in ICU were significantly shorter than those of the control group(P<0.05).Conclusion　Bronchoscopy helps to control the infections of ventilator associated pneumonia after the lung radical resection, improves the clinical symptoms, and promotes the rehabilitation of patients.]]> 2017/7/13 10:29:15 LIU Song-tao, SHEN Bin, LEI Tao, et al. 441true ［Abstract］　Objective　To explore the rehabilitative effect of operative timing on intertrochanteric fractures in elderly patients.Methods　Forty elderly patients with intertrochanteric fractures in our hospital were collected as the research subjects from January 2013 to May 2015. They were randomly divided into two groups in which the early surgery group(n=20) had an operation within one week and the later surgery group(n=20) had an operation more than one week. The outcomes including the incidence of post-operative complications, the quality of life, the hip joint function and the fatality rate were compared between the two groups after a follow-up of one year.Results　The incidence of post-operative complications in the early surgery group(15.0%) was significantly lower than that in the latter surgery group(45.0%)（P<0.05）. No significant differences were found between the two groups in ADL scores and hip joint function before the operation(P>0.05). ADL scores were （55.16±11.19） and （42.77±12.99）, and the hip joint function scores were （77.16±7.58） and （65.11±6.97) in the early surgery group and the later group 6 months after the surgery respectively, with a significant difference between the two groups(P<0.05). There were no significant differences in ADL scores and the hip joint function scores 12 months after the surgery(P>0.05). Three cases died in the the early surgery group(15%), and 4 cases died in the later surgery group(20%) one year after the operation(χ²=0.173, P>0.05).Conclusion　Early surgery is associated with the decreased post-operative complications, especially for preventing infectious complications and pressure ulcer in long-term bed-ridden patients, and therefore improves the quality of life and the hip joint function 6 months after the surgery. Population-based studies are needed to confirm the impact of timing of surgery on the fatality rate.]]> 2017/7/13 10:29:15 XU Lei, FANG Shi-yuan, SUN Jian-wan, et al. 440true ［Abstract］　Objective　To explore the efficacy and safety of the pentobarbital sodium in the miniature pig anesthetic model without endotracheal intubation.Methods　Twenty miniature pigs were randomly divided into group A(n=10) and group B(n=10). Group A was given 3% pentobarbital sodium injection, and group B was given 5% ketamine injection to perform anesthesia and the same operation without endotracheal intubation. The anesthetic effect, surgical effect, vital signs and adverse reactions were compared between the two groups.Results　The anesthetic and surgical effects were satisfying in both of the two groups. The induction time of group A was significantly longer than that of group B［(10.76±1.50) vs (8.73±1.15)］. There were no significant differences between the two groups in the weight, breathing rate, heart rate, anal temperature, pulse, oxygen saturation, and recovery time(P>0.05). No obvious adverse reactions were found in group A. There were some adverse reactions in group B during the anesthesia, including more secretion of saliva, higher muscle tension and chew phenomenon, and intraoperative vomiting in one case. No dead case was found in the two groups.Conclusion　Both pentobarbital sodium and ketamine can be used for anesthesia in the miniature pigs without endotracheal intubation. Pentobarbital sodium is more safe, economic and convenient for the anesthesia.]]> 2017/7/13 10:29:15 FAN Wen-ju, LI Pei-feng, LIU Gang, et al. 439true ［Abstract］　Objective　To construct the plasmid recombinant plasmid pMDl8-KIM-1 as a standard for detecting the mRNA expression of KIM-1 by real-time fluorescence quantitative PCR.Methods　A 131 bp DNA segment of KIM-1 gene cDNA was amplified by PCR, and purified KIM-1 PCR fragments from RT-PCR cloned into pMDl8-T vector, then transformed into bacterium DH5α. The recombinant plasmid DNA extracted from positive clones was identified by PCR amplification and sequence determination. Positive clones were purified and accurately quantified, and a 10-fold serial dilution of the recombinant plasmid DNAs was used as the calibrator.Results　The target segment was successfully recombined into pMDl8-T vector, and the length of the amplified target fragment of KIM-1 was 131 bp. The objective gene sequence was in accordance with that provided by the NCBI genbank.Conclusion　The recombined standard plasmid for KIM-1 gene real-time PCR analysis is constructed successfully.]]> 2017/7/13 10:29:15 CHEN Zhi-zhong, QING Ji-lin, YANG Wen-hui, et al. 438true ［Abstract］　Objective　To observe the enhancement patterns of benign and malignant thyroid hypoechoic nodules.Methods　The ultrasonic diagnosis of 111 thyroid hypoechoic nodules was retrospectively analyzed. The enhancement patterns were recorded and were compared with the pathological results.Results　The pathological results demonstrated that 68 nodules were malignant and 43 were benign. Most malignant nodules showed inhomogeneous enhancement in the ultrasonic results（55/68）, while most benign ones showed ring enhancement（28/43）.Conclusion　Contrast-enhanced ultrasound is helpful to differentially diagnose benign and malignant thyroid hypoechoic nodules.]]> 2017/7/13 10:29:15 CHEN Xue-xue, WANG Xiao-yan, LING Bing, et al. 437true ［Abstract］　Objective　To explore the influence of valve replacement in the same period of bipolar radiofrequency ablation with beating heart on posterior parallel circulation(PPC).Methods　Sixty patients with valve disorders complicated with chronic atrial fibrillation were collected continuously as the research subjects and were divided into group A and group B according to different operation methods. Group A(n=33) received valve replacement with beating heart plus bipolar radiofrequency ablation. Group B(n=27) received valve replacement with beating heart alone.The variables including the intvacardiac procedure time, cardio pulmonary by pass(CPB) time and posterior parallel circulation(PPC) time were compared between the two groups.Electrocardiogram and echocardiogram were performed to monitor the recovery of cardiac function after the surgery, and the changes of left ventricular ejection fraction(LVEF) and the success rate of bipolar radiofrequency ablation were compared between the two groups.Results　All the patients underwent the operation successfully without atrioventricular block and were discharged from the hospital in good condition. There was no significant difference in the intvacardiac procedure time between the two groups(P>0.05). The CPB time and PPC time of group A were significantly shorter than those of group B(P<0.05). Cardiac function was improved in both of the two groups after the operation. The rate of EF change of group A was significantly higher than that of group B at postoperative 6 months(P<0.05), however there was no significant difference in cardiac function between the two groups 6 months after the operation(P>0.05). The trend graph was characterized with a faster increase and a larger peak value in group A than those in group B. The success rate of bipolar radiofrequency ablation of group A was 88%（29/33）, and most of the patients(27/29) could keep sinus rhythm at postoperative 12 months. The success rate of bipolar radiofrequency ablation of group B was 11%（3/27).Conclusion　Valve replacement in the same period of bipolar radiofrequency ablation with beating heart is safe and effective in improvement of cardiac function with reduced CPB time and PPC time.]]> 2017/8/4 9:07:51 SHEN Bin, LI Xiang-wei, WEN Zhao-ke, et al. 436true ［Abstract］　Objective　To longitudinally study the effect of highly active anti-retroviral therapy(HAART) on the expression levele of antiviral protein MxA mRNA in peripheral blood mononuclear cells(PBMCs) of HIV mono-infected and HIV/HCV co-infected patients and to explore the relationship between the baseline of MxA mRNA level in HIV/HCV co-infected patients after HAART and the efficacy of subsequent anti-HCV with interferon.Methods　Forty-two cases of HIV mono-infected patients and 33 cases of HIV/HCV co-infected patients were recruited as the objects of this study and 22 healthy volunteers were enrolled in the control group. All patients were collected from the Eighth People′s Hospital of Guangzhou, whose CD4⁺ T<200 cells/mm³ before HAART. Before anti-HCV therapy, the levels of MxA mRNA expression in PBMCs of HIV/HCV co-infected patients at different time points of HAART(0 W, 4 W, 12 W, 24 W, 48 W, 72 W, 96 W) were observed in this study. The relationship between the therapeutic effect of subsequent anti-HCV and MxA mRNA was analyzed. Early viral response(EVR) and non early viral response(NEVR) were used to evaluate the effect of ant-HCV in this study.Results　The levels of MxA mRNA in the PBMCs of HIV mono-infected group were decreased 4 weeks after HAART(P<0.05), while there were no significant differences among the other time points of HAART in the same group and between the HIV mono-infected group and the control group(P>0.05). There were no significant differences in the HIV/HCV co-infected group in and between it and the control group among all time points(P>0.05). After the HAART of 96 weeks in anti-HCV therapy for HIV/HCV co-infected patients, the MxA mRNA levels before anti-HCV therapy were significantly higher in the EVR group than those in the NEVR group(P<0.05).Conclusion　Long-term HAART has no significant effect on the expression levels of MxA mRNA in PBMCs of HIV/HCV co-infected patients, however, the levels of MxA mRNA after HAART and before anti-HCV therapy can be one of the predictors of anti-HCV effect for HIV/HCV co-infected patients.]]> 2017/8/4 9:07:51 GONG Lan, PAN Neng-lang, CHEN Zhen-yu, et al. 435true ［Abstract］　Objective　To observe the correlation between plasma hsa-miR-30a and left ventricular hypertrophy（LVH） in hypertension patients and to study whether plasma hsa-miR-30a can be a diagnostic marker for patients with LVH or not.Methods　Seventy-three cases with LVH were collected from the hypertensive patients as LVH group and 77 cases without LVH as the control group. The mRNA expression of hsa-miR-30a in the derived plasma was analyzed by real-time PCR. Evaluation of the sensitivity and specificity of hsa-miR-30a on the diagnosis of LVH was performed by analyzing the ROC curve. The association between plasma hsa-miR-30a level and ventricular wall thickness was assessed by Pearson correlation analysis.Results　Compared with that in the control group, the level of hsa-miR-30a expression in LVH group was significantly higher［（5.16±0.31）（2^-Δct×10⁴）vs （14.28±0.92）（2^-Δct×10⁴）, P=0.000］. A receiver operating characteristic(ROC) curve showed that the areas under the curve(AUC) for the plasma level of hsa-miR-30a were 0.932. The optimal hsa-miR-30a cut-off value on receiver operating characteristic curve was 7.267（2^-Δct×10⁴）. The corresponding cut-off points with sensitivity and specificity were 94.5% and 89.6%, respectively. Pearson correlation analysis indicated that the level of hsa-miR-30a expression in plasma was positively associated with IVSd and LVPWd )(r=0.636 and 0.637, P=0.000 and 0.000, respectively).Conclusion　Plasma hsa-miR-30a is elevated in hypertension patients with LVH and positively associated with left ventricular wall thickness. Elevated plasma hsa-miR-30a level is significant for the diagnosis of LVH in hypertension patients.]]> 2017/8/4 9:07:51 PAN Wei, ZHAO Juan, YANG Ze-fu, et al. 434true ［Abstract］　Objective　To observe the efficacy and safety of autologous peripheral blood stem cell transplantation(APBSCT) in multiple myeloma(MM).Methods　Ten patients with MM received APBSCT. The peripheral stem cells were mobilized with cyclophosphamide(CTX) plus granulocyte colony stimulating factor(G-CSF) and were collected with CS-3000 Cell Separator and preserved in a refrigerator at -80 ℃. After the pretreatment of melphalan at 200 mg/m², the peripheral blood stem cells were defrosted and reinfused into the patients.Results　The median mononuclear cells and CD34⁺ cells were 4.54(2.31～9.80)×10⁸/kg and 4.17(2.03～7.76)×10⁶/kg, respectively. All patients were engrafted. The median time of neutrophil count>0.5×10⁹/L and platelet count>20×10⁹/L were 13.3(9～16)days and 15(11～19)days, respectively.(3)Eight patients had complete response(CR) and two patients had very good partial response(VGPR) three months after the transplantation. With a median follow-up of 54.6(3～111)months, eight patients survived and six patients were progression-free survival(PFS). Four patients relapsed. After active treatment, two patients were CR and two patients died of relapse. Infections were the major complications. There was not transplant-related mortality.Conclusion　APBSCT with cryopreservation peripheral stem cells at -80 ℃ is safe and effective for MM. The short-term effects are remarkable, but the disease needs a long-term maintenance treatment after the transplantation.]]> 2017/8/4 9:07:51 LAN Mei, LIN Jin-ying, TANG Yang-ming, et al. 433true ［Abstract］　Objective　To study the clinical effects and clinical values of pig tail catheter(disposable drainage catheter) with negative pressure drainage and traditional chest closed drainage in the treatment of pneumothorax.Methods　Seventy-eight patients with pneumothorax were divided into two groups by the random number table method.The treatment group(n=39) was treated by pig tail catheter with negative pressure drainage, and the control group(n=39) was treated by traditional chest closed drainage. The clinical effects and adverse reaction were compared between the two groups.Results　The treatment group had a better clinical effect, shorter operation time and shorter length of operative incision than the control group(P<0.05).Conclusion　Pig tail catheter with negative pressure drainage is safe and effective in the treatment of pneumothorax.]]> 2017/8/4 9:07:51 CHEN Ying, LIN Wan-li, GONG Lan-juan, et al. 432true ［Abstract］　Objective　To study the characteristics of hemoglobin electrophoresis in HbBart′s hydrops syndrome.Methods　HbBart′s hydrops syndrome was checked out in 39 patients by capillary electrophoresis, and their thalassemia genes were analyzed. The patients were divided into three groups according to different gestational weeks: 24～28 pregnant weeks group, 29～32 pregnant weeks group and 33～38 pregnant weeks group. The contects of HbBart′s and HbPortland were detected and compared among the three groups.Results　The values of HbBart′s and HbPortland were （78.2±2.5）%,（14.3±2.5）%,（79.9±4.8）% and （12.7±4.0）%,（84.0±2.1）%,（9.3±2.5）% in the 24～28 pregnant weeks group, the 29～32 pregnant weeks group, and the 33～38 pregnant weeks group, respectively. There were significant differences in the contects of HbBart′s and HbPortland among the three groups(P<0.05). All cases were homozygous Southeast Asian(SEA) type deletion except that one was Thailand combined SEA type deletion.Conclusion　HbBart′s and HbPortland are the main components of HbBart′s hydrops syndrome, and capillary electrophoresis is a diagnostic method for HbBart′s hydrops syndrome.]]> 2017/8/4 9:07:51 WEI Jin-hua, WAN Li-kai, LI You-qiong, et al. 431true ［Abstract］　Objective　To observe the effect of propofol combined with cis-atracurium on muscle relaxation in pediatric patients with fiberoptic bronchoscopy.Methods　The clinical data of 68 pediatric patients with fiberoptic bronchoscopy were collected from January 2013 to December 2014 and were retrospectively analyzed. They were divided into group A and group B according to the different anesthetic medications. Group A(n=36) was given propofol combined with cis-atracurium ammonium and group B(n=32) was given propofol combined with ketamine. The time of producing effectiveness, the block maintenance time, the duration of action and the recovery index were compared between the two groups.Results　The time of producing effectiveness was significantly less in group A than that in group B. The block maintenance time and the duration of action were significantly longer in group A than those in group B. The recovery indexes of muscle relaxation in group A were significantly elevated than those in group B(P<0.01).Conclusion　Propofol combined with cis-atracurium has better effect of muscle relaxation than propofol combined with ketamine in anesthesia for pediatric patients with fiberoptic bronchoscopy.]]> 2017/8/4 9:07:51 LIANG Shu-min, LIN Ying, TANG Mao 430true ［Abstract］　Objective　To investigate the effect of (-)-epigallocatechin-3-gallate(EGCG) on SCL-1 cell proliferation and the regulation mechanisms.Methods　SCL-1 cells were treated with 20 μm, 60 μm or 100 μm EGCG. The proliferation was detected by MTT when cells were treated with EGCG for 24 h. Western blot was used to detect the expressions of PCNA, PTEN and p-AKT.Results　In the EGCG-treated groups, the cell proliferation rates of SCL-1 cells were (84.25±3.3)%, (71.34±2.3)% and (60.33±4.5)%, when the cells were treated with EGCG at 20, 60 and 100 μm respectively. There were significant differences between the EGCG-treated groups and the control group［(100.01±2.7)%］(P<0.05). The expression of PCNA was significantly decreased when the cells were treated with EGCG, moreover, it significantly increased the expression of PTEN (P<0.05), and obviously decreased the p-AKT levels(P<0.05).Conclusion　EGCG inhibits the proliferation of SCL-1 cells through PTEN-PI3K-AKT signaling pathway.]]> 2017/8/4 9:07:51 LI Li-li, CHEN Xian-feng, HUANG Qi-tao, et al. 429true ［Abstract］　Objective　To investigate the effects of extracorporeal shock wave combined with veronica peregrina on BMP-2 and VEGF mRNA expressions in the callus of fracture nonunion in rabbits, and to explore its mechanism.Methods　The model of radius fracture nonunion was made in 120 New Zealand white rabbits, which were then randomly divided into four groups: group A in the treatment of extracorporeal shock wave combine with veronica peregrina, group B in the treatment of veronica peregrina, group C in the treatment of extracorporeal shock wave and group D as the model control. The tested animals were treated with extracorporeal shock wave, and the two ends of the nonunion frocture were shocked 800 times at 0.5 mj/mm² and a frequency of 70～80 times/minute. The samples were collected in each group at the end of 48 hours, 1, 2,4, 6, 12 weeks respectively after the treatment. Immunohisto chemistry stain was performed to examinate the expressions of BMP-2 and VEGF mRNA in the callus.Results　（1）There were no significant differences in the expressions of BMP-2 mRNA in callus at each time point among the four groups 48 hours after the treatment. At the end of 1, 2, 4, 6 and 12 weeks, group A and group C had significant difference compared with group D（P<0.05）. Group B had no significant difference compared with group D(P>0.05). Group A had significant difference compared with group B and C(P<0.05).（2）Group A,group B and group C had significant differences in the expression of VEGF mRNA in callus at each time point compared with group D（P<0.05）. Group A had significant difference compared with group B and group C（P<0.05）.Conclusion　The treatment of extracorporeal shock wave combined with veronica peregrina can upregulate both the expressions of BMP-2 and VEGF mRNA, stimulate osteogenesis and improve the healing of fracture nonunion.]]> 2017/8/8 16:30:41 ZHAO Zi-xing, LI Hong-yu, XI Li-cheng, et al. 428true ［Abstract］　Objective　To explore the effect of sodium valproate(VPA) on the expressions of glial fibril protein(GFAP) in hippocampus of rats with epiltic brain injury.Methods　Eighty adult male SD rats were randomly divided into group A, group B, group C and group D, with 20 cases in each group. No intervention was given to group A; group B was only treated with VPA gastric lavage; Epileptic rat′s models were established in both group C and group D, with the treament of valproate in group D and without any intervention in group C. The number of CA1 positive cells in CA2 and GFAP regions, the average cross-sectional area, the average gray value, the accumulated optical density, and the average optical density were compared among the four groups.Results　The numbers of GFAP positive cells in CA2 and CA were significantly higher in group C and group D than those in group A and group B, and the number of GFAP cells in group D was significantly lower than that in group C. The mean cross-sectional area of CA1 or CA2, was significantly higher in group C or group D than that in group A or group B, and the mean area of CA1 or CA2 in group C was higher than that in group B. The average gray values were significantly lower in group C and group D than group A and group B, and the one in group C was lower than that in group B.Conclusion　Valproate can significantly inhibit the expression of GFAP in rat model of epilepsy, and reduce the degree of brain damage.]]> 2017/8/8 16:30:41 LU Wang-ke, GAO Ran 427true ［Abstract］　Objective　To investigate the clinical effect of refractive correction alone on preschool childrens′ vision.Methods　One hundred and twelve ametropic children(224 eyes) aged from 2 to 7, received reasonable refractive correction and their clinical data were retrospectively analyzed. The changes of visual acuity in each case were recorded before and after the optical treatment at the end of three and six months, and statistical analysis was perfomed with SPSS13.0 soft ware.Results　There were significant differences in visual improvement among the three different age groups three months after the optical treatment(χ²=29.391, P=0.000). The 3 to 5 age group had the most remarkable improvement, followed by the 5 to 7 age group and younger than 3-year-old group. However, there were no significant differences in visual improvement among the three age groups six months after the optical treatment(χ²=8.210, P=0.084). When divided into different groups according to different refractive status, the children showed the best improvement of visual acuity six months after the optical treatment in the simple hyperopia or hyperopic astigmatism group, followed by the compound hyperopic astigmatism group, the mix astigmatism group, the simple myopic or myopic astigmatism group, and the compound myopic astigmatism group. There were significant differences in visual improvement among the groups(χ²=23.517, P=0.003).Conclusion　Refractive correction alone improves preschool childrens′ corrected visual acuity, especially for those with simple hyperopia or hyperopic astigmatism and for those aged from 3 to 5 years.Some ametropic children may achieve normal visual acuity after the optical treatment.]]> 2017/8/8 16:30:41 LAN Fang-fang, ZHAO Wu-xiao, GAN Lu 426true ［Abstract］　Objective　To study the clinical effect of the simple nasal balloon on otitis media with effusion(OME) in children.Methods　Forty children aged from 3 to 12 years with OME were divided into two groups. The control group(n=20, 38 ears)received traditional Valsava maneuvers and medication. The nasal balloon assistant treatment group(n=20, 36 ears) received insufflation of the eustachian tube by nasal balloon and medication. The tymanometry and curative efficacy were compared between the two groups 2～3 months after the treatment.Results　In the control group, 20 ears completely recovered, 10 ears improved, and 8 ears invalid, with a curative rate of 52.63% and an efficacious rate of 78.95%. In the nasal balloon assistant treatment group, 27 ears recovered completely, 5 ears improved, and 4 ears invalid, with a curative rate of 75.00% and an efficacious rate of 88.89%. There was a significant difference in the curative rate between the two groups(P<0.05).Conclusion　The simple nasal balloon assistant treatment is a safe and effective therapy for children with secretory otitis media.]]> 2017/8/8 16:30:41 JING Yan, PENG Lu, CHEN Rui-chun, et al. 425true ［Abstract］　Objective　To explore the risk factors of lung infection in patients with stroke by using Meta-analysis.Methods　The relevant papers were searched in PubMed, EBSCO, Cochrane, CNIK and Wanfang databases from January 2010 to August 2015. The papers were evaluated and the data were extracted. Stata software was used to analyze the data.Results　Eight papers with 606 cases and 3 104 controls were included. The Meta-analysis showed that elder age, diabetes mellitus, smoking, dysphagia, disturbance of consciousness were the independent risk factors of lung infection in patients with stroke. Gender had no significant association with lung infection in patients with stroke.Conclusion　Elder age, diabetes mellitus, smoking, dysphagia and disturbance of consciousness are the risk factors of lung infection in patients with stroke.]]> 2017/8/8 16:30:41 HU Rui-ting, OU Shi-ning, QIN Dong-hua, et al. 424true ［Abstract］　Objective　To establish the clinical nursing pathway(CNP) of whole lung lavage(WLL) in pneumoconiosis patients, and to evaluate the effect of the application.Methods　Fifty cases of WLL were divided into the experimental group and the control group according to the different hospitalization orders, with 25 cases in each group. The CNP nursing mode was implemented in the experimental group and the traditional nursing mode was implemented in the control group. The implementation effect of the two groups was compared between the two groups.Results　The average length of hospital stay and the average cost of hospitalization in the experimental group were significantly less than those in the control group. The degree of patients′ satisfaction of the experimental group was higher than that of the control group(P<0.01). The clinical symptoms scores after the lavage were significantly lower than those before the lavage in both of the two groups(P<0.05). Lung ventilation functions showed a tendency to improve 3 days after the lavage compared with those before the lavage in both of the two groups, but without significant differences(P>0.05). Mental health status of the patients who were treated with CNP were better than the patients who were not treated with CNP(P<0.05).Conclusion　Implementation of CNP can reduce the hospitalization costs, shorten the hospital stay and improve the satisfaction and mental health status, and improve the quality of nursing in the pneumoconiosis patients who accept WLL.]]> 2017/8/8 16:30:41 LI Dong-hong, LIU Jian, CHEN Zhi-jun, et al. 423true ［Abstract］　Objective　To investigate the expression patterns and the significances of distant metastasis and prognosis of MiR-3182 innasopharygeal carcinoma(NPC).Methods　The expressions of MiR-3182 in 89 cases with nasopharygeal carcinoma were detected by real time RT-PCR. The relation between the expression and distant metastasis of NPC and its prognosis were analyzed.Results　The expressions of MiR-3182 were found in all of the tissues of nasopharygeal carcinoma. The expression level of MiR-3182 in nasopharygeal carcinoma in the group with distant metastasis was significantly higher than that in the group without distant metastasis（P<0.01）. The expression of MiR-3182 in the group with DFS<3 years was significantly higher than that in the group with DFS≥3 years（P<0.01）.Conclusion　MiR-3182 may play a key role in distant metastasis of nasopharygeal carcinoma and is associated with poor prognosis.]]> 2017/8/10 9:01:20 ZHOU Min-yan, LAN Gui-ping, SI Yong-feng, et al. 422true ［Abstract］　Objective　To detect the expressions of 8-hydroxydeoxyguanine(8-OhdG) and phospho-epithelial growth factor receptor(p-EGFR) in tongue squamous cell carcinoma(TSCC) and their correlation.Methods　The expressions of 8-OHdG and p-EGFR in 30 specimens of tongue squamous cell carcinoma tissues and adjacent-carcinoma tissues were detected by immunohistochemistry. The correlation between 8-OHdG expression and p-EGFR expression was analyzed by Spearman rank correlation analysis.Results　Both 8-OHdG and p-EGFR were highly expressed in the TSCC tissues, and their positive rates were 80.0%, 56.7% respectively, whereas the low expressions which were shown with positive rate of 16.7%, 6.7% respectively, were observed in the adjacent-carcinoma tissues. The expressions of 8-OHdG and p-EGFR in carcinoma tissue were significantly higher than those in the adjacent-carcinoma tissue(P<0.05), and the statistically significant correlation was observed between 8-OHdG expression and p-EGFR expression(P<0.05).Conclusion　Reactive oxygen species express in oral cancer, which is positively correlated to p-EGFR.]]> 2017/8/10 9:01:20 QIN Xiang-ming, CHEN Lei, YU Da-hai, et al. 421true ［Abstract］　Objective　To study the expressions of miR-378 and FOXO1 in hepatocellular carcinoma(HCC) and the correlation of the expressions between miR-378 and FOXO1 genes.Methods　Matched normal and cancerous tissues from 8 patients with HCC were examined for miR-378 and FOXO1 expressions using real-time quantitative PCR. Independent-Samples T test was used to test the significance of the means of the expressions of miR-378 and FOXO1 between normal and cancerous tissues. The correlation of the expressions between miR-378 and FOXO1 in cancerous tissues was detected by bivariate correlation analysis.Results　The expressions of miR-378 and FOXO1 in HCC cancerous tissues were both significantly lower than those in adjacent tissues(P=0.0106 and P=0.000623). The expression of miR-378 was positively correlated with that of FOXO1(r=0.541, P=0.031) in cancerous tissues.Conclusion　The expressions of miR-378 and FOXO1 in HCC tissues are significantly lower than those of the adjacent tissues. The abnormal expressions of miR-378 and FOXO1 and their interaction network may play a vital role in HCC development.]]> 2017/8/10 9:01:20 QIN Bin, LIU Shun, QIU Xiao-qiang, et al. 420true ［Abstract］　Objective　To investigate the relationships between sperm morphology and sperm motility parameters.Methods　One hundred and forty-nine semen samples were tested. Sperm motility parameters were analyzed by computer-aided sperm analysis(CASA), and the sperm morphology was observed by Diff-Quik rapid staining. Sperm morphology and motility were classified based on the “WHO laboratory manual for the examination and processing of human semen”(Fifth Edition). Some of the samples were morphologically normal(normal group, n=75) and the others were morphologically abnormal(abnormal group, n=74).Results　VCL, VSL, VAP, ALH and BCF were significantly higher in the morphologically normal group than those in the abnormal group(P<0. 01), but there were no significant differences in LIN, WOB and STR between the two groups(P>0.05). There were significantly positive correlations between the morphologically normal sperm rates and VCL, VSL, VAP, ALH, BCF. The percentage of the sperms that only had the abnormal heads was positively correlated with VSL, VAP, VCL, LIN, STR, ALH and BCF. The percentage of multiple abnormalities sperms was negatively correlated with VSL, VAP, VCL, LIN, STR, ALH and BCF.Conclusion　There are correlations between sperm morphology and sperm motility parameters. Abnormal sperm morphology influences the movement velocity, the space displacement and the movement way of sperms. The sperms with only head deformity are not the decisive factors affecting the sperm motility rate and the degree of space displacement. Multiple abnormalities may be one of the main reasons affecting sperm motility.]]> 2017/8/10 9:01:20 MAO Xian-bao, XUE Lin-tao, HE Bing, et al. 419true ［Abstract］　Objective　To study the expression of transforming growth factor - beta 1(TGF-β1) and it clinical singnificances in female breast fibroadenoma.Methods　Twenty pairs of fresh tissue specimens including the tumor tissue and the matched normal tissue were collected from the female patients with breast fibroadenoma and were preserved in liquid nitrogen. The reverse transcription quantitative polymerase chain reaction(RT-qPCR) was used to detect the levels of TGF-β1 mRNA l in the samples. Student′s t-test was used to analyze the data.Results　The expression of TGF-β1 mRNA was significantly elevated in the breast fibroadenoma tissues than that in the normal tissues(P=0.004).Conclusion　 As an important factor for the development of stroma, TGF-β1 is involved in the formation process of fibroadenoma.]]> 2017/8/10 9:01:20 GUO Ju-jiang, LIAO Hong-ye, WENG Yi-yin, et al. 418true ［Abstract］　Objective　To study the value of dynamic contrast enhanced MRI（DCE-MRI） in the diagnosis of primary hepatocellular carcinoma.Methods　Thirty-nine patients with liver local masses examined by DCE-MRI and verified by pathological findings were divided into 2 groups. The primary hepatocellular carcinoma group(n=27) and the nontumorous nodule group(n=12). Tissue-4D software was used for the post-processing of the DCE-MRI images and the tissue volume perfusion parameters including transfer constant（Ktrans）, extravascular extracellular space volume（Ve） and rate constant（Kep） were calculated.Results　There were statistically significant differences in Ktrans and Ve between the two groups. The ROC curve had moderate diagnostic value. When the best diagnostic threshold of Ktrans for hepatocellular carcinoma was 0.298 min^-1, the sensibility was 0.926 and the specificity was 0.750. When the best diagnostic threshold of Ve for hepatocellular carcinoma was 0.237, the sensibility was 0.926 and the specificity was 0.500.Conclusion　DCE-MRI can quantificationally analyze microcirculation of the lesion. It provides an effective method for identifying benign and malignant nodules.]]> 2017/8/10 9:01:20 KONG Yuan, LI Wen-mei 417true ［Abstract］　Objective　To explore the clinical effects of Jieyoushen long-acting antibacterial material combined with compound radix arnebiae oil in the treatment of burn wound.Methods　One hundred and twenty-eight patients with burn wound were randomly divided into two groups. The control group(n=63) was treated with compound radix arnebiae oil, and the observation group(n=65) was treated with Jieyoushen long-acting antibacterial material combined with compound radix arnebiae oil. The time of wound healing, hospital stay, the pain of burn wound after treatment, the rate of concrescence, the ratio of germirates and the clinical effect were compared between the two groups.Results　For both of the superficial second degree burns and deep second degree burns, the time of wound healing and hospital stay in the observation group were signigicantly shorter than those in the control group(P<0.01). The pain of burn wound after treatment in the observation group was lighter than that in the control group(P<0.01). The positive ratio of germirates in the observation group was lower than that in the control group, while the rate of concrescence was higher in the observation group(P<0.05). The clinical effect in the observation group was better than that in the control group(P<0.05).Conclusion　Jieyoushen long-acting antibacterial material combined with compound radix arnebiae oil can shorten the time of wound healing and hospital stay, reduce wound infection, relieve pain in the treatment of burn wound.]]> 2017/8/10 9:01:20 QIN Qiu-hai, ZHU Shao-ban, CHEN Jie, et al. 416true ［Abstract］　Objective　To explore the effects of Shengjing capsules in combination with Levocarnitine on DNA fragmentation index(DFI) and acrosome intact rate of sperms and outcomes in vitro fertilization cycles.Methods　The DFI, acrosome intact rate and clinical outcomes of 322 infertility male patients were analysed retrospectively according to the different treatment protocals. Group A(n=96) was treated with Shengjing capsules, and group B(n=103) received Levocarnitine. Group C（n=123） was given Shengjing capsules combined with Levocarnitine. The three groups were performed in vitro fertilization(IVF) three months after the treatment.Results　(1)The sperm DNA integrity rate, sperm acrosome intact rate, fertilization rate, good-quality embryo rate and the clinical pregnancy rate in group C were significantly higher than those in group A and group B(P<0.05).（2)There were no significant differences in the cleavage rates among the three groups(P>0.05). (3)The sperm acrosome intact rate, fertilization rate, good-quality embryo rate, clinical pregnancy rate in group A were significantly higher than those in group B(P<0.05). There was no significant difference in sperm DNA integrity rate between group A and group B(P>0.05).（4）The clinical pregnancy rate was 51.2%, 45.8% and 39.8% in group C, group B and group A respectively(P>0.05).Conclusion　Sperm acrosome intact rate, good-quality embryo rate and clinical pregnancy rate in infertility male patients treated with Shengjing capsules were improved better than those with Levocarnitine; the sperm DNA integrity rate, acrosome intact rate, fertilization rate, good-quality embryo rate and clinical pregnancy rate were improved better in the patients treated with Shengjing capsules in combination with Levocarnitine than those with Shengjing capsules or Levocarnitine.]]> 2017/8/10 17:32:15 HE Yong-zhi, LI Da-wen, HE Bing, et al. 415true ［Abstract］　Objective　To observe the curative effect of hyperbaric oxygen combined with purslane speedwell herb on treating bone defects, and to investigate the mechanism of the treatment.Methods　80 New Zealand rabbits were selected to make a bone defect model and then they were randomly divided into four groups. Group A was treated by hyperbaric oxygen combined with purslane speedwell herb. Group B received the therapy of hyperbaric oxygen. Group C was treated with purslane speedwell herb. Group D was taken as the control. The rabbits were sacrificed evenly in each group at the end of the second, 4^th, 8^th and 12^th weeks respectively. The general observation, X-ray, the detection under light microscope and electron microscope were performed, and the results were compared between the two treatment methods.Results　X-ray showed that group A had the maximum amount of the formation of callus, followed by group B, C and group D. Under the electron microscope, osteoblast organelles in group A were more well-developed than those in group B, C and D, and the collagen fibers of group A arranged regularily and distributed evenly.Conclusion　Hyperbaric oxygen combined with purslane speedwell herb is more effective in the treatment of bone defects than hyperbaric oxygen alone or herbal treatment alone. It can significantly enhance the ability of proliferation and the differentiation of osteoblast, and accelerate the growth of callus obviously, which provides a new method for the clinical treatment of bone defects.]]> 2017/8/10 17:32:15 XI Li-cheng, LI Hong-yu, ZHAO Zi-xing, et al. 414true ［Abstract］　Objective　To investigate the effects of self-made CPAP device on arterial oxygen pressure and intrapulmonary during one-lung ventilation(OLV).Methods　40 patients with ASA grade ⅠorⅡand a schedule for pulmonary lobectomy surgery and one-lung ventilation were randomly divided into 2 groups:CPAP group(group A, n=20) and matched group(Group B, n=20). In group A, CPAP in 5 cmH₂O was administered to the non-ventilated lung by using the self-made device during OLV. Group B did not received any intervention during OLV. Arterial and mixed venous blood samples were taken for gas analysis before OLV(T₁) and at 30 min(T₂), 60 min(T₃) after OLV. Intrapulmonary shunt(Q_S/Q_T) was calculated.Results　There was no significant difference in PO₂ between the two groups at T₁(P>0.05). PaO₂ in group A was higher than that in group B at T₂ and T₃(P<0.01). However, there were no significant differences in Q_S/Q_T between the two groups at T₁, T₂ and T₃(P>0.05).Conclusion　Using the self-made device to add 5 cmH₂O CPAP can effectively improve arterial oxygenation during OLV, but does not affect the intrapulmonary shunt (Qs/Qt).]]> 2017/8/10 17:32:15 YU Mei-gang, HUANG Yu-ting, MO Yi-jie, et al. 413true ［Abstract］　Objective　To investigate the clinical effect of standard triple therapy combined with Xiaojingua powder in eradication of Helicobacter pylori(Hp).Methods　Seventy-five patients with chronic gastritis caused by HP in our hospital from July 2014 to July 2015 were divided into three groups according to the random number table method. Group A(n=25) was given the standard triple therapy; group B(n=25) and group C(n=25) were treated with oral Xiaojingua powder based on the treatment of western medicine. The eradication of Hp and compliance of the drug use were compared among the three groups two weeks after the treatment and thirty days after the discontinuation of medicine.Results　After the treatment, the eradication rates in both group B(88%) and group C(84%) were significantly higher than that in group A(72%)(P<0.01）. There was no significant difference in the eradication of Hp between group B and group C（P>0.05). The medication compliance rates were 92%, 88% and 84% in group A, group B and group C, without significant differences among the three groups(P>0.05).Conclusion　Xiaojingua powder in combination with the standard triple therapy is convenient in use and increases the eradication rate of Hp in patients with chronic gastritis.]]> 2017/8/10 17:32:15 GAN Hai-qiu, JIAN Zhuo-huan, TANG Hua-feng, et al. 412true ［Abstract］　Objective　To study the interventional effect of aspirin combined with clopidogrel on symptomatic mild carotid artery stenosis.Methods　One hundred and twenty-six patients with symptomatic carotid artery stenosis in our hospital from April 2013 to November 2014 were taken as the research subjects and randomly divided into the combination group(n=63) and the control group(n=63). All the patients were treated with oral clopidogrel, 75 mg/d. The combined group was treated with aspirin(100 mg/d) at the same time. The two groups were treated for 3 months. The curative effects and incidence of adverse reactions were compared between the two groups. The carotid intima media thickness(IMT) was compared before and after the treatment in both of the two groups.Results　28 cases were markedly effective, 24 cases effective and 11 cases ineffective in the combination group while, 18 cases were markedly effective, 23 cases effective and 22 cases ineffective in the control group. There was a significant difference in curative effect between the two groups(P<0.05); After the medication, the IMT values of both the two groups decreased significantly（P<0.05); There was no significant differenc in IMT between the combination group(0.75±0.12)mm and the control group（0.79±0.15）mm(P>0.05). There was no significant difference in the incidence of adverse reactions between the combination group(47.6%) and the control group(33.3%)（P>0.05）.Conclusion　Aspirin combined with clopidogrel is safe and effective in the treatment of symptomatic mild carotid artery stenosis.]]> 2017/8/10 17:32:15 GONG Yu, WEN Dan, SONG Zhi-jie 411true ［Abstract］　Objective　To summarize the indications and the complications of transabdominal chorionic villus sampling(TA-CVS) in the first trimester of pregnancy.Methods　60 women with single pregnancy came from the outpatient department of obstetrics in our hospital. Their average age was (29.27± 4.1)years, and their average gestational age were (12.61±0.86)weeks. The indications of the procedure included the suspicion of the fetal chromosomal disorder(20 cases), and coupling with the same type thalassemia carrier(42 cases). The body temperature, blood pressure, and pulse were measured before the TA-CVS. The samples of villi were sent to check the karyotypes, or detect the thalassemia genotypes.Results　The homozygote or double heterozygote of β-thalassemia were detected in 6 cases, severe α-thalassemia in 8 cases, and hemoglobin H disease in 6 cases. Autosomal trisomy was detected in 3 cases out of 7 fetus edema/cystic hydroma by B ultrasound. Miscarriage occurred in 1 case two weeks after doing the procedure(1.67%).Conclusion　Early diagnosis, timely intervention and low fetal-loss are the advantages of TA-CVS for invasive prenatal examination. The main indications of TA-CVS are the prenatal diagnosis of single genetic disease, and chromosomal disorders especially when the ultrasound findings show the suspicious disorders in the first trimester of pregnancy.]]> 2017/8/10 17:32:15 TIAN Mao, QIN Ting, ZHANG Ji-hong, et al. 410true ［Abstract］　Objective　To analyze the changes of carotid artery elasticity in the patients with obstructive sleep apnea hypopnea syndrome(OSAHS) after the treatment with ET Technique.Methods　Thirty patients with OSAHS were selected and given individualized treatment for three consecutive months. The intima-media thickness(IMT) was measured by high frequency linear ultrasound probe. An ultrasonic echo-tracking method was used to evaluate common carotid artery elastic modulus, including pressure-strain clastic modulus(Eρ), stiffness parameter(β), arterial compliance(AC), one point pulse wave velocity(PWVβ) and augment index(AI).Results　After the treatment, Eρ,βand PWVβ decreased and AC increased compared with those after the treatment（P<0.01）. AI decreased to some extent after the treatment, but no difference was found before and after the treatment（P>0.05）.Conclusion　ET can accurately detect the changes of carotid artery elasticity in the patients with obstructive sleep apnea hypopnea syndrome before and after the treatment. The changes of ET parameters can guide the clinical treatment of OSAHS and indicate atherosclerosis in an early stage.]]> 2017/8/30 17:15:12 HE Yan, WANG Xiao-yan, ZHANG Bing, et al. 409true ［Abstract］　Objective　To investigate the expression of cluster of differentiation-24(CD24) and its correlation with pathological types and clinical stages in cervical carcinoma.Methods　Forty patients with cervical cancer and 35 patient with cervical intraepithelial neoplasia(CIN) were collected as experimental group 1 and experimental group 2, respectively, other 20 females with normal cervical were considered as the control group. Streptavidin-peroxidase immunohistochemical technique was used to detect CD24.Results　CD24 protein was mainly located in cellular membrane and cytoplasm of malignant cells. Positive rate was 90%(36/40) in cervical cancer and 42.9%(15/35) in CIN. No positive expression of CD24 was found in the normal cervical tissues. There were significant differences among the three groups(P<0.05). The positive rate of CD24 protein was not correlated with the pathological types and clinical stages(P>0.05).Conclusion　The expression of CD24 is significantly higher in cervical carcinoma and CIN than that in the normal cervical tissues. The higher expression of CD24 is not associated with the histopathological types and the clinical stages.]]> 2017/8/30 17:15:12 WU Yu-ying, LU Yan-qiong, CHEN Chang-yi, et al. 408true ［Abstract］　Objective　To explore the efficacy of laparoscopic conservative surgery combined with medication of mifepristione in the treatment of unruptured tubal pregnancy.Methods　A total of 96 cases with tubal pregnancy in our hospital from April 2013 to March 2015, were randomly divided into two groups. The study group(n=48) was treated with modified laparoscopic conservative surgery combined with medication of mifepristone. The control group was treated with conservative laparoscopic surgery. The operative time, blood loss, hospital stay, blood HCG and the patency of oviduct were compared between the two groups.Results　(1)The operative time, hospitalization days and blood loss of the study group were significantly shorter or less than those of the control group(P<0.05). (2)The postoperative blood HCG drop rate and the negative conversion rate of blood HCG 2 weeks after the surgery were higher in the study group than those in the control group(P<0.05). (3)There were no significant difference in the postoperative tubal obstruction between the study group and the control group(P>0.05).Conclusion　Modified laparoscopic surgery combined with medication of mifepristone is effective in preventing the occurrence of persistent ectopic pregnancy, and retains the integrity and function of the ociduct to the greatest extent.]]> 2017/8/30 17:15:12 XUE Lian, XUE Su-qing, LI Yuan-hong 407true ［Abstract］　Objective　To explore the clinical therapeutic effect of electrically stimulating right median nerve on the patients with craniocerebral traumatic coma, and to provide a new therapeutic method and theoretical basis for the treatment of craniocerebral traumatic coma.Methods　Eighty-nine patients with craniocerebral traumatic coma in our hospital from November 2013 to November 2014 were randomly divided into the control group and the observation group. The control group(n=43) was given the conventional treatment methods including intracranial decompression, nerve nourishing, infection prevention, prevention and treatment of stress ulcer, maintain water and electrolyte balance symptomatic support and rehabilitation training. The observation group(n=46) was given the same conventional treatment method as the control group besides the use of electrically stimulating right median nerve simultaneously. The GCS score, quality of life, language function and movement function were compared between the two groups after a follow up of 3 months.Results　The quality of life, language function, movement function and Glasgow prognostic assessment improved significantly in the observation group than those in the control group after a follow up of 3 months（P<0.05）.Conclusion　Electrical stimulation of right median nerve can promote the nervous functional recovery, reduce the degree of disability and improve the quality of life for the patient with craniocerebral traumatic coma.]]> 2017/8/30 17:15:12 LIANG Wen-hai 406true ［Abstract］　Objective　To analyze the efficacy of percutaneous vertebroplasty(PVP) combined with Gushuning tablets in the treatment of thoracolumbar vertebral fracture and the influence on the incidence of secondary fracture after surgery.Methods　The clinical data of the patients with thoracolumbar vertebral fractures were collected in our hospital from January 2013 to August 2015. All patients in the study received PVP operation and then they were divided into two groups according to the different postoperative treatments, the medication group(n=48) was treated with Gushuning tablets and the non-medication group(n=47) was not treated with Gushuning tablets. The general conditions, the VAS score before and after the surgery, the recurrence rate of fracture and deformity were compared between the two groups.Results　VAS scores were decreased in both of the two groups after the treatment of PVP. There were no significant differences in VAS scores between the two groups before and after the operation(P>0.05). The postoperative recurrence rate of fracture and the rate of deformity in the medication group were significantly lower than those in the non-medication group(P<0.05).Conclusion　Gushuning tablets can reduce the postoperative recurrence rates of fracture and deformity for patients with thoracolumbar vertebral fractures after PVP operation.]]> 2017/8/30 17:15:12 CHEN Tao, LI Guan-bao, JIA Shi-qing, et al. 405true ［Abstract］　Objective　To evaluate the prevalence of anemia and the growing development of middle school students of Zhuang nationality in Longan, Guangxi, and to provide the evidence for anemia prevention and treatment in Longan county.Methods　Two thousand and fifty-one middle school students aged from 12 to 18 years were sampled. Their height, weight and hemoglobin levels were measured and analyzed.Results　The total prevalence rate of anemia was 14.48%. Boys′ prevalence rate of anemia was 9.21%, and 20.04% for girls. Microcytic hypochromic anemia was the main type of anemia. Girls were at higher risk for anemia than boys(OR=2.470, 95%CI: 1.904～3.204, P<0.001). The total prevalence rate of malnutrition was 27.74%. The students with anemia were at higher risk for malnutrition than those without anemia(33.33% vs 26.80%, OR=1.366, 95%CI: 1.050～1.778, P=0.02).Conclusion　Anemia is still a serious health problem among the middle students of Zhuang nationality in Longan county, which affects the growth and development of the students. Treatment for anemia should be strengthened among the adolescence with malnutrition.]]> 2017/8/30 17:15:12 LU Jing-huan, LU Feng-ji, CHEN Gui-rong 404true ［Abstract］　Objective　To observe the dynamic changes of IL-22 in the process of ventricular remodeling after acute myocardial infarction(AMI) and to explore the effect of IL-22 on ventricular remodeling.Methods　Sixty AMI patients diagnosed by coronary angiography were collected as the experimental group, and other 30 patients without coronary heart disease were taken as the control group. At the time points of 2 hours, 2 weeks and 4 weeks, the left ventricular function was detected with color doppler echocardiography and the serum concentration of IL-22 was determined with ELISA, and IL-22mRNA was determined with RT-PCR.Results　The left venticular function and left ventricular remodeling declined in AMI patients. The serum concentration and mRNA expression of IL-22 increased with time prolonging and were significantly higher in the experimental group than those in the control group at different time points.Conclusion　The dynamic changes of IL-22 level and mRNA expression exist in patients with AMI. IL-22 may play a role in ventricular remodeling after AMI.]]> 2017/9/1 11:34:47 SHI Lei, LIU Shi-huan, LIU Ling, et al. 403true ［Abstract］　Objective　To explore the potential target genes of miR - 1284 and its possible molecular mechanisms on gastric cancer development.Methods　The bioinformatics software was used to predict the target genes of miR-1284. The slow virus mediated miR-1284 transfection MGC-803 gastric cancer cells were taken as the miR-1284 group(LV-miR-1284). The empty carrier slow virus vector transfection cells were taken as the negative control group and the untransfected slow virus carrier cells as the blank control group. Fluorescence quantitative PCR was used to detect the expression of target genes EIF4A1and Western blot was used to detect EIF4A1 protein expression and its target genes were verified by dual luciferase EIF4A1.Results　Compared with those in the negative control group and the blank control group, the expressions of gene and protein of EIF4A1 were decreased in the miR-1284 group, Dual luciferase test demonstrated that miR-1284 could combine with target genes EIF4A1 3 ′UTR.Conclusion　miR-1284 plays a role in biological functions by combining with its target gene EIF4A1.]]> 2017/9/1 11:34:47 ZHAN Ze-xu, WEI Wei-yuan, CAO Wen-long, et al. 402true ［Abstract］　Objective　To investigate the value of compression and /or invasion signs in ultrasonic diagnosis of minimal benign and malignant hepatic tumors.Methods　178 hepatic lesions less than 2.0 cm in 164 cases proved by puncture or operation pathology were examed by the dimensional ultrasonography and color Doppler flow. The compression and /or invasion signs were regarded as a criterion of malignant hepatic tumors.Results　The detection rate of compression and /or invasion signs in the malignant hepatic minimal lesions(75.24%，79/105) was much higher than that in the benign hepatic minimal lesions（4.11%，3/73）(P<0.05). The sensitivity, specificity and accuracy of the ultrasound in the diagnosis of malignant hepatic minimal tumors were 75.24%, 95.89% and 83.71% respectively. The Kappa was 0.679, and the area of ROC was 0.846（95%CI=0.786～0.906）.Conclusion　The parameters of compression and /or invasion signs in ultrasonography can be used for the differential diagnosis of benign and malignant hepatic minimal lesions.]]> 2017/9/1 11:34:47 XU Chun-mei, LIU Tian-qi, CHEN Yan-hong, et al. 401true ［Abstract］　Objective　To investigate the etiologic characteristics of the adult patients with community-acquired pneumonia(CAP) in the urban and rural areas of Guangxi Zhuang Autonomous Region, and facilitate the development of normalized diagnosis and treatment of CAP.Methods　A prospective study was performed on 889 adult patients with CAP at 14 centers in Guangxi Zhuang Autonomous Region from January 2008 to December 2010. All the patients were divided into urban area group and rural area group according to the different geography distributions. The etiologic characteristics and the distribution of detected pathogens were compared between the two groups.Results　486 strains of pathogens were isolated from 422 patients with qualified serum samples and/or sputum cultures, including Mycoplasma pneumoniae(138/486,28.40%), Haemophilus infulenzae(88/486,18.11%), Streptococcus pneumoniae(77/486, 15.84%), Klebsiella pneumoniae(53/486,10.91%) and Chlamydia pneumoniae(43/486,8.94%). Mycoplasma pneumonia infection and Pseudomonas aeruginosa infection were higher in the population of the rural area group than those in the urban area group(41.12% vs 19.72% and 5.58% vs 1.73%), while the proportions of Streptococcus pneumoniae infection and legionella pneumophila infection were lower in the rural area group than those in the urban area group(8.1% vs 21.11% and 3.05% vs 7.96%). In the CAP patients, bacteria(excluding legionella pneumophila) were responsible for the infection in the urban group, and atypical pathogens were responsible for the infection in the rural group.Conclusion　Mycoplasma pneumoniae is the most common pathogen that leads to adult CAP in Guangxi Zhuang Autonomous Region. Haemophilus infulenzae and Streptococcus pneumoniae are still the most popular pathogenic bacteria for CAP as well. Atypical pathogens are the most common pathogens in the urban areas but bacteria in the rural areas.]]> 2017/9/1 11:34:47 JIANG Ling-yu, QIN Zhi-qiang, GAO Xing-hua, et al. 400true ［Abstract］　Objective　To systemically assess the recurrence rate and survival rate of complete mesocolic excision(CME) for colon cancer.Methods　The controlled studies which were related to the treatment of traditional surgery and CME for colon cancer and published in the last eight years were retrieved from the electronic databases(China National Knowledge Infrastructure, Wanfang Database, PubMed, Medline, Ovid, Embase, Cochrane ISI Web of Knowledge, Cochrane Database of Systematic Reviews). With strictly screening according to the standard, the quality of studies was evaluated. Selective trials′ data were analyzed by the Review Manager 5.1 software.Results　A total of five nonrandomized clinical trials were finally included, involving a total of 1 804 patients in the research(558 patients in CME group and 1 246 patients in control group). The 5-year postoperative recurrence rate of CME was 9.99% on average. Meta-analysis showed that the postoperative disease-free survival and overall survival in CME group were significantly higher than those in the control group（P<0.05）.Conclusion　CME is safe and effective in accordance with the principles of oncology therapy and is expected to become a new standardized operation technique for colon cancer.]]> 2017/9/1 11:34:47 WANG Long, LI Lei, MAI Wei, et al. 399true ［Abstract］　Objective　To compare the clinical application value between hydroxyl chromium bovine package film and polyester film in the treatment of congenital ventricular septal defect(VSD).Methods　A total of 50 cases with congenital heart disease were enrolled from April 2013 to April 2014 in our hospital and were divided into two groups: bovine package group(n=25) and polyester group(n=25). The bovine pericardium group received the repair surgery with glutaraldehyde-fixed bovine pericardium path. The polyester group received the repair surgery with terylene. All the patients were followed up for 1 to 3 years, the clinical results were analyzed and compared between the two groups.Results　After the treatment, life signs of the 50 patients were stable. All the symptoms including fever, cough and gastrointestinal symptoms were disappeared. The incidences of adverse reactions respectively were 12.0% and 40.0% in the bovine package group and in the polyester group, with a significant difference(P<0.05).Conclusion　The hydroxyl chromium bovine pericardium patch has a better clinical effect and less complications on repairing congenital ventricular septal defect than polyester film.]]> 2017/9/1 11:34:47 DENG Hai-qing, DU Zheng-long, LING Yi, et al. 398true ［Abstract］　Objective　To explore the feasibility of establishing an experimental rabbit model of mucociliary transport system of maxillary sinus.Methods　Twenty maxillary sinuses of 10 rabbits were included in this research. Local anaesthesia was performed. The size of bone window at the anterior wall of maxillary sinus was 1 cm×0.8 cm. Transport pathway of methylthioninium chloride was observed by trasnasal endonasal endoscope for 1.5 hour.Results　All experiments were smoothly finished under the local anaesthesia. The bone window size of 1 cm×0.8 cm could be satisfactory for all experimental manipulations. Methylthioninium chloride was transported from inferior wall to anterior-inferior edge of the maxillary natural ostium directly, and then transported out of maxillary sinus at that site.Conclusion　White rabbits can be established to be experimental model of the research related to maxillary mucociliary transport system.]]> 2017/9/1 11:34:47 XU Cheng-li, GONG Jian-gu, LIANG Jian-ping, et al. 397true ［Abstract］　Objective　To research the clinical efficacy of aspheric Toric intraocular lens（IOL） in the treatment of cataract patients with corneal astigmatism.Methods　One hundred and seventeen age-related cataract patients(158 eyes) with more than 1.0 diopter(D) of preexisting corneal astigmatism were divided into two groups. The Toric group（92 eyes in 69 cases） received the aspheric Toric IOL implantation after phacoemulsification, and the control group（66 eyes in 48 cases） received the aspheric IOL. The postoperative symptoms, uncorrected visual acuity（UCVA）, total astigmatism and objective visual quality indicators were examined.Results　With an average follow-up of 15.2 months, the total astigmatism in the Toric group（0.65±0.38）D was significantly lower than that in the control group three months after the operation （1.84±0.46）D, while the UCVA in Toric group（0.80±0.23） was higher than that in the control group（0.59±0.14）. The postoperative contrast sensitivity, MTF cut off value, visual acuity at 100% contrast, visual acuity at 20% contrast and the improvement of subjective visual quality in the Toric group were higher than those in the control group. The mean rotation of IOLs in the Toric group was （7.1±4.2）degrees.Conclusion　Aspheric Toric IOL can provide good subjective and objective visual quality for cataract patients with corneal astigmatism.]]> 2017/9/6 16:16:49 LI Li, LAN Qian-qian, WANG Lu 396true ［Abstract］　Objective　To explore the prenatal diagnosis of chromosome abnormality by detecting free DNA in maternal amniotic fluid by using massively parallel genomic sequencing technology.Methods　The amniotic fluid samples from 60 pregnant women were collected from December 2013 to May 2014 at the People′s Hospital of Guangxi Zhuang Autonomous Region. Those pregnant women had a gestational age of 18 to 24 weeks and high risks of Down syndrome and/or fetal abnormalities shown by color Doppler ultrasound. The amniotic fluid samples were drawn from the pregnant women and their amniotic fluid DNAs were extracted for preparing a sequencing library. By using Ion Proton, high-throughput sequencing procedure was carried out. The sequencing data were compared with the human reference gene-database and statistically analyzed. Simultaneously, the amniotic fluid cells were cultured for chromosomal karyotyping which compared with the massively parallel genomic sequencing technology.Results　By massively parallel genomic sequencing, in 3 out of 60 cases showed chromosome copy number abnormalities, 57 cases have no obvious abnormity. By the standard of chromosomal karyotypic analysis, there were 6 chromosomal abnormalities.Conclusion　Massively parallel genomic sequencing is highly consistent with karyotypic analysis in sensitivity and specificity for prenatal diagnosis of the fetus chromosome abnormalities by detecting amniotic fluid DNA in pregnant women and has great advantages of high accuracy, high throughput, high sensitivity, and low cost in clinical practice.]]> 2017/9/6 16:16:49 HUANG Li, HE Bing, WANG Shi-kai, et al. 395true ［Abstract］　Objective　To investigate the expression and changes of cytoglobin（Cygb） gene on hypoxic neurons in vitro.Methods　The primary cortical neurons from newborn rats were cultured in a serum-free culture system. The neurons were identified by polyclonal antibody against neuron specific enolase on the 6th day and were randomly divided into 4 groups for assessing cell viability by CCK8 and for observing the gene expression of Cygb by Real-time PCR at 0 h, 8 h, 16 h, 24 h after hypoxic injury was induced with the concentration of oxygen between 0.5% and 0.9%.Results　The purity of neurons was more than 99%; Expression of RNA Cygb was increased in a time-dependent manner, and there was significant difference in each group(P<0.05).Conclusion　The expression of Cygb in neurons increases rapidly after hypoxic injury indicating that Cygb may have an important function in the process of protecting hypoxic neuron injury.]]> 2017/9/6 16:16:49 YANG Han-hua, MA Hai-ran, XIAO Dan-ping, et al. 394true ［Abstract］　Objective　To evaluate the clinical value of detecting antisperm antibodies of seminal plasma and serum to distinguish obstructive azoospermia from idiopathic azoospermia.Methods　The clinical data of 204 cases with azoospermia and testicular appendage surgical exploration in our hospital from January 2010 to December 2013 were retrospectivly analyzed. According to the results of the surgical exploration,the patients were divided into group A(idiopathic azoospermia, n=106) and group B(obstructive azoospermia, n=98). The antisperm antibodies of seminal plasma and serum before the detection were compared between the two groups, and the clinical value of antisperm antibodies in the seminal plasma and serum was analyzed to differentiate the obstructive azoospermia from the idiopathic azoospermia.Results　There were 9.31% of the all 204 azoospermia patients with the antisperm antibodies in serum positive, and 6.86% in seminal plasma; these data were respecticaly 7.55% and 4.72% in group A, among which IgG-AsAb were 2.83% and 1.89%, IgM were 1.89% and 0.94, IgA were 2.83% and1.89%. and the data were respecticaly 11.22% and 9.18% in group B, among which IgG-AsAb were 6.12% and 4.08%, IgM were 3.06% and 2.04%, IgA were 2.04% and 3.06%. There were no significant differences in the counterpart indicators between the two groups(P>0.05).Conclusion　Antisperm antibody positively expresses in both the serum and seminal plasma of azoospermia patients. However the sensitivity is not high for the antisperm antibody in seminal plasma and serum to differentiate the obstructive azoospermia from the idiopathic azoospermia. The clinical types of azoospermia can not be differentiated by detecting the antisperm antibodies in seminal plasma or serum.]]> 2017/9/6 16:16:49 LIU Huang, ZHANG Zhi-xing, ZHENG Hou-bin, et al. 393true ［Abstract］　Objective　To observe the clinical efficacy of proprioceptive neuromuscular facilitation(PNF) combined with hyperbaric oxygenation in the treatment of spinal cord injury complicated with incomplete paraplegia.Methods　A total of 48 patients with spinal cord injury complicated with incomplete paraplegia were randomly divided into group A and group B, with 24 cases in each group. All the patients were treated with decompression of spinal cord and internal fixation of spine. All the patients started to receive rehabilitation care 1 to 2 weeks after the surgery. Group A used PNF combined with hyperbaric oxygenation therapy, while group B used routine exercise therapy and acupuncture. All the patients received rehabilitation care for 6 months. The sensation and motion before and after the treatment were evaluated according to the scaling criteria of Chinese Spinal Cord Injury Association, and the activities of daily living were evaluated according to Modified Barthel Index(MBI).Results　The sensation and motion scores and MBI indices were more significantly elevated in both of the two groups after the treatment than those before the treatment(P<0.05). The increasing extents of sensation and motion scores and MBI indices were significantly greater in group A after the treatment than those in group B(P<0.05).Conclusion　PNF combined with hyperbaric oxygenation in the treatment of spinal cord injury complicated by incomplete paraplegia is helpful for the recovery of spinal cord function, and significantly improves the motor ability and self-care ability.]]> 2017/9/6 16:16:49 LIANG Bin, CHU Ye, YIN Dong, et al. 392true ［Abstract］　Objective　To investigate the correlation between the different courses of senile insomnia and mild cognitive impairment（MCI）, and to provide new research ideas for the early prevention of Alzheimer′s disease(AD).Methods　Two hundred and forty senile patients with insomnia(≥60 years old) were treated in Guangxi national hospital from July 2013 to August 2014, and divided into three groups: acute, subacute, and chronic insomnia groups. Other 80 senile people without insomnia were taken as the control group. Pittsburg sleep quality index scale(PSQI), montreal cognitive assessment（MoCA）, and the quality of life questionnaire(SF-36) were evaluated.Results　(1)There were statistically significant differences in the scores of PSQI among the four groups(P<0.05). For the daytime function, group C>group B>group A, D; for the other indicators, group C>group B>group A>group D. (2)There were statistically significant differences in the scores of MoCA among the four groups(P<0.05). For the space, language and abstract function, group A, D>group B>group C; for the overall cognitive function, naming, attention, delayed recall and directional function, group D>group A>group B>group C. (3)The MCI prevalence rates in the four groups were 16.25%, 47.50%, 67.50% and 12.50% respectively. For the MCI prevalence rates, group C>group B>group A, D. (4)There were statistically significant differences in the scores of SF-36 among the four groups(P<0.05). For the body pain score, group C>group B>group A>group D; for the social function, group A, D>group B>group C; for the other indicators, group D>group A>group B>group C.Conclusion　There is a positive correlation between the length of the course of senile insomnia and the prevalence rate of MCI. Due to the short period of the disease, acute insomnia has little influence on prevalence rate of MCI and cognitive function.]]> 2017/9/6 16:16:49 ZHONG Liang, LIU Li, ZHONG You-an, et al. 391true ［Abstract］　Objective　To observe the changes and clinical value of tumor necrosis factor alpha（TNF-α） in the patients with obstructive sleep apnea hypopnea syndrome(OSAHS) complicated with hypertension(HT).Methods　A total of 157 OSAHS patients diagnosed between August 2013 and September 2015 were recruited, with 57 cases in OSAHS group and 100 cases in OSAHS+HT group, and 15 healthy people were taken as the control group. All the groups were monitored by polysomnography, and the apnea hypopnea index(AHI), the lowest blood oxygen saturation(LSaO₂) and mean blood oxygen saturation(MSaO₂) were oberved. The serum TNF-α in the patients was detected by double antibody sandwich enzyme linked immunosorbent assay(ELISA).Results　TNF-α levels in OSAHS group and OSAHS+HT group were significantly higher than those in the control group(P<0.05), and TNF-α levels in OSAHS+HT group were significantly higher than those in OSAHS group(P<0.05). Compared with those in the control group, MSaO₂ and LSaO₂ in OSAHS group and OSAHS+HT group were significantly decreased(P<0.05). The systolic blood pressure and diastolic blood pressure at the first admission to the clinic in OSAHS group and OSAHS+HT group were significantly higher than those in the control group(P<0.05), and the blood pressure in OSAHS+HT group was significantly higher than that in OSAHS group(P<0.05). The correlation analysis suggested that there were significantly positive correlations between AHI and TNF-α in both OSAHS group and OSAHS+HT group(P<0.05), and that there were significantly negative correlations between AHI, TNF-α and MSaO₂ and LSaO₂(P<0.05).Conclusion　The incidence of OSAHS and OSAHS complicated with hypertension may be associated with the increased levels of inflammatory factor TNF-α.]]> 2017/7/4 9:03:44 LIANG Da-hua, XIE Yu-ping, LIU Jian-hong, et al 390true ［Abstract］　Objective　To investigate the expressions and clinical significances of serum matrix metalloproteinase-9(MMP-9) and interleukin-35(IL-35) in patients with acute cerebral infarction.Methods　Forty-four patients with acute cerebral infarction(ACI) were collected as ACI group, and 40 healthy people were taken as the control group. The serum levels of MMP-9 and IL-35 were detected and compared between the two groups.Results　The serum level of IL-35 in ACI group was significantly lower than that in the control group. The serum level of MMP-9 in ACI group was significantly higher than that in the control group（all P<0.05）.Conclusion　Cerebral infarction is closely associated with inflammatory reaction. MMP-9 and IL-35 may play a critical role in the mechanism of ischemical inflammation injury of cerebral infarction.]]> 2017/7/4 9:03:44 LI Yan-hua, WEI Jun-jie, FAN Bing-lin, et al 389true ［Abstract］　Objective　To investigate the expression of CD71 in renal tissue of humans with IgA nephropathy and the relationship with clinical pathology, and to explore a possible biomarker for diagnosis and prognosis of IgA nephropathy.Methods　180 patients with IgA nephropathy by renal biopsy were collected as the research subjects(IgA nephropathy group), and other 18 patients with renal calculi or renal tuberculosis but without IgA nephropathy were taken as the control group. The expressions of CD71 detected by light-microscopy, immunohistochemistry, immunofluorescence and IgA nephropathy semi-quantitative score were compared between the two groups. The relationship of CD71 with both pathologic severity and clinical relevance was analyzed.Results　(1)The expression of CD71 in the IgA nephropathy group was significantly higher than that in the control group(P<0.05), and the expression intensity of CD71 was elevated with the aggravation of kidney pathological damage;(2)The expressions of CD71 were positively related to mesangial cell proliferation index(MsHI) and mesangial matrix increase index(MsMI)(P<0.05);(3)The expressions of CD71 were positively related to albuminoria, but negatively related to creatinine clearance rate(CCR)(P<0.05), and were not correlated with the levels of hematuria(P>0.05).Conclusion　The expressions of CD71 are different in different pathological renal tissues of IgA nephropathy, which are elevated with the increase of pathological damage, and are positively related to MsHI and MsMI. In addition, the expressions of CD71 are negatively related to CCR,and positively related to albuminuria,suggesting that CD71 is relevant to the decline in renal function and prognosis. The expression of CD71 may be one of the biological markers to judge the severity and prognosis of IgA nephropathy.]]> 2017/7/4 9:03:44 XIE Ying, YI Wei, ZHA Yan, et al 388true ［Abstract］　Objective　To investigate the value of nasopharyngeal biopsy under general anesthesia in the diagnosis of submucosal nasopharyngeal carcinoma.Methods　Eight outpatients who were suspected to have nasopharyngeal carcinoma and underwent nasopharyngeal biopsy under local anesthesia for three times but failed to be diagnosed as nasopharyngeal carcinoma were given nasopharyngeal biopsy with nasal endoscope in endotracheal intubation for general anestheaia.Results　Eight cases who recieved endoscopic nasopharyngeal biopsy under general anesthesia were diagnosed as nasopharyngeal carcinoma.Conclusion　Nasopharynx biopsy surgery under general anesthesia should be recommended for the patients who are suspected to have nasopharyngeal carcinoma but fail to be definitely diagnosed by nasopharyngeal biopsy under local anesthesia.]]> 2017/7/4 9:03:44 QIN Yang-da, WENG Jing-jin, WANG Yong-li, et al 387true ［Abstract］　Objective　To observe the clinical and pathological characteristics of percutaneous renal biopsy(PRB) in patients with acute renal failure(ARF).Methods　A total of 84 patients with ARF admitted to our hospital between January 2006 and January 2016 were selected as the study subjects, all of whom were diagnosed by PRB. The accuracy rates of pathology for the judgment of ARF were compared before and after PRB. The adjustment of the treatment plan after PRB and the cure rate were recorded, and the safety of implementing PRB was evaluated.Results　The causes of the disease of 60 cases among the study subjects were determined before PRB, accounting for 71.43%. Compared with the postoperative pathology, the diagnosis in 56 cases of 60 cases were correct and the accurate rate was 93.33%. Before PRB, there were 24 cases whose causes of the diseases were unknown. Among them, the causes of the diseases in 21 cases were determined by PRB, including 12 cases of renal tubular lesions, 7 cases of acute interstitial nephritis and 2 cases of crescent glomerulonephritis. After PRB, the causes of the diseases in 81 cases were known, accounting for 96.43%. Compared with those before PRB(71.43%), the diagnosis rate after PRB was significantly higher(χ²=19.461, P<0.01). After the pathological diagnosis by PRB, the treatment plans were unadjusted in 40 cases, but adjusted in 34 cases and were replaced by other treatments in 10 cases, including tumor induced ARF treated with anti-tumor intervention and proliferative sclerotic nephritis treated with long-term hemodialysis and vascular fistulaforming intervention. One month after the treatment, 49 cases were cured, accounting for 58.33%. After PRB, 2 cases had the complications of mild gross hematuria, and there were spontaneous remission without any intervention. One case had the complications of severe hematuria, and the hemoglobin decreased by 10 g/L. The incidence of complications was 3.57%.Conclusion　The accurate diagnosis of acute renal failure is the basic premise of clinical treatment. The implementation of percutaneous renal biopsy can significantly improve the diagnosis rate. Adjusting the treatment plan according to the results of pathological biopsy makes the clinical treatment beneficial and the safety of the implementation of the percutaneous renal biopsy is relatively higher.]]> 2017/7/4 9:03:44 LIU Qing-hua, XIE Shou-cheng, XUE Shao-qing 386true ［Abstract］　Objective　To explore the effects of different sutures on ovarian sexual hormones secretion in laparoscopic operation.Methods　Sixty patients with unilateral ovarian cyst who underwent laparoscopic cystectomy were randomly divided into two groups: hilum continuous U style suture group(group A) and cortex continuous suture group(group B), with 30 cases in each group. The patients in group A received hilum continuous U style suture for hemostasis, and the patients in group B received cortex continuous suture for hemostasis. The serum levels of follicle stimulating hormone(FSH), luteinizing hormone(LH), estradiol(E₂) were detected respectively before the operations and at the first and the third month after the operation.Results　In the two groups,compared with those before the operation, the level of E₂ reduced significantly, and the levels of FSH and LH increased the first month after the operation(P<0.05); the level of E₂ reduced slightly, the levels of FSH and LH increased slightly at the third month after the operation with no significant differences(P>0.05); group B had more influence on the ovarian sexual hormone secretion than group A（P<0.05）.Conclusion　Both of the two hemostatic methods have effects on sexual hormones secretion in laparoscopic cystectomy for ovarian endometriotic cyst. In most of the patients, the ovarian sexual hormone secretion can be recovered to the normal level basically within 3 months after the operation.]]> 2017/7/4 9:03:44 LIU Yuan-yuan 385true ［Abstract］　Objective　To examine the surface topography of different arch wire segments after slide by canine tooth brackets during Damon-Q self-locking braces using an atomic force microscope, and to explore the best combination of pairing self-ligating bracket and arch wire.Methods　Two malocclusion patients were recruited who had four first premolars extracted and were treated with a Damon-Q self-locking brace. The upper left canine teeth were retracted to contact the second premolar on segments of 0.018″×0.025″ stainless steel arch wires(thick wire group) and segments of 0.016″×0.022″ stainless steel arch wires(filament group) during the treatment. Three-dimensional structures of the four surfaces of arch wire segment that slid by the canine tooth bracket and posterior arch wire segment which was between the upper left bicuspid and first molar and had not been slid by any bracket were scanned using an atomic force microscope before and after canine teeth slid. The three-dimensional parameters were obtained from the topography data. Independent samples T test or Mann-Whitney U test was used for statistical analysis on SPSS13.0.Results　(1)Upper and lower surfaces of the arch wire that had contacted the slot walls of canine tooth bracket in the thick wire group became smoother. The internal narrow surface that was at the base of the slot became rougher. The external narrow surface that was not contacted the bracket slot was not significant. The lower surface of the arch wire that had contacted the slot walls of canine tooth bracket in the filament group became smoother. The internal narrow surface that was on the base of the slot became a little rougher, and the upper surfaces of the arch wire and external narrow surface that was not contacted the bracket slot was not significant. (2)The upper surface of the arch wire segments between the second bicuspid and first molar in the thick wire group became a little smoother and the external narrow surface of the arch wire segments in the filament group became a little rougher after the canine tooth slid.Conclusion　(1)Slide back and forth over the canine fangs, the internal narrow surface of thick and thin arch wire that is on the base of the slot becomes rougher while the structures on the other three sides are unchanged or slightly smooth. (2)From the view of structural changes on the surface of the wire, the segments of 0.018″×0.025″ or 0.016″×0.022″ stainless steel arch wires can be paired with Damon-Q self-locking braces before closing extraction space for similor effect.]]> 2017/7/4 9:03:44 FU Li-li, LU Shi-ying, HUANG Min-fang, et al 384true ［Abstract］　Objective　To investigate the blood lead levels and its influencing factors in 1 to 7-year-old children in Dongxing city.Methods　One to seven-year-old children were selected as the study subjects by using stratified cluster sampling method. The enrolled children received the treatment in Dongxing maternal and child health hospital from January 2013 to December 2015 and lived in Dongxing city for more than 1 year. The data of their personal circumstances, family environment and presence of clinical symptoms were collected by questionnaire survey, and the levels of blood lead in peripheral blood were detected.Results　In all the 1 015 children enrolled, the mean levels of blood lead were (81.16±19.47)μg/L, in whom the lead levels of 59 cases(5.81%) were more than 100 μg/L. The highest blood lead level was in the industrial zones, followed by those in the downtown and suburban areas. The lowest level is in the rural areas. Multiple regression analysis and stepwise regression analysis showed that TV factory, gas station, garage and new housing were the environmental risk factors of high blood lead.Conclusion　The blood lead level of the 1 to 7-year-old children living in Dongxing city is less than that of the national average, however, if their blood lead levels are on normal high level, the children will have more symptoms like those of lead poisoning. It is necessary to perform lead-eliminating treatment for the childred in early stage.]]> 2017/7/4 9:03:44 LI Ding-feng, ZHANG Hai-xia, HUANG Chao-gui, et al 383true ［Abstract］　Objective　To compare Ligasure techniques and conventional suture techniques in abdominal radical hysterectomy of cervical cancer.Methods　The clinical data of 193 patients with the stage Ⅰa2～Ⅱa of cervical cancer in the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2012 to October 2015 were retrospectively analyzed, among whom 98 cases who received the treatment of abdominal radical hysterectomy with Ligasure were selected as the observation group, and the other 95 cases who received the treatment of abdominal radical hysterectomy with the conventional suture were selected as the control group. The indexes of operative time, blood loss during the operation, hospital stay and the amount of lymphatic drainage were compared between the two groups.Results　The indexes of operative time, blood loss during operation, hospital stay and the amount of lymphatic drainage in the observation group were significantly lower than those in the control group(P<0.05).Conclusion　The Ligasure vessel sealing system can significantly reduce the time of operation, the length of hospital stay, the blood loss during operation and the amount of lymphatic drainage in the treatment of transabdominal radical hysterectomy. It has the advantages of safety, hemostatic reliability and residual free foreign substance in the body.]]> 2017/7/4 9:03:44 LIANG Lan, ZHAO Ren-feng, HUANG Wei-long 382true ［Abstract］　Objective　To detect the dental maxillofacial changes in female adults with class Ⅲ Jaw′s malocclusion being treated with fixed appliance combining microimplant anchorage.Methods　The female adult patients with class Ⅲ Jaw′s malocclusion were recruited. All cases were 18 to 39(24.1±5.98) years old. Their occlusional relationships of canine and first molar in two sides were full mesiocclusions,with an average angle ANB of -6.43°, and angle SN-MP of 33.47°. They were all treated with fixed appliance combining microimplant anchorage in mandibular buccal shelf area and the functional tongue muscle training was done by the patients themselves during the treatment. Lateral cepholometric values were compared by paired t-test in SPSS18.0 before and after the treatment.Results　The angles SNA, ANB, and H and Lip-Diff turned to be larger, while the angle SNB and U1-NA, the values APDI and LL-Sn⊥FH turned to be smaller（P<0.05） after the treatment.Conclusion　Counterclockwise rotation of the mandibular plane, vertical control of posterior teeth and distal large moving of lower dentition in female adult patients with class Ⅲ Jaw′s malocclusion can be obtained by the treatment with fixed appliance combining microimplant anchorage in mandibular buccal shelf area and functional tongue muscle training which diminish the Jaw′discrepancy in sagittal plane and benifit the control to the mandibular plane angle, and therefore improve esthetic profile, in the patients after the treatment.]]> 2017/7/4 9:03:44 ZHOU Yan, HUANG Min-fang, FANG Zhi-xin, et al 381true ［Abstract］　Objective　To investigate the multifunctional reservation neck dissection in the treatment of differentiated thyroid cancer(DTC).Methods　Sixty patients with DTC were randomly divided into the control group and the treatment group, with 30 cases in each group. The treatment group was treated with modified neck dissection. While the control group was treated with multifunctional reservation neck dissection. The surgical results and complications were compared between the two groups.Results　No operative deaths occurred, with a normal appearance in the neck and a good recovery in both of the two groups. The patients were followed up for 2 years but no recurrence and deaths were found. The incidence of complications in the treatment group was significantly lower than that of the control group(P<0.05).Conclusion　Multifunctional reservtion neck dissection is safe and effective in the treatment of differentiated thyroid cancer. It can effectively preserve the feeling of ear area, the neck and the supraclavicular region.]]> 2017/7/4 9:03:44 HUANG Lin, DENG Wei, LAO Jing-mao, et al 380true ［Abstract］　Objective　To observe the effect of sodium hyaluronate in combination with acupuncture and massage on treating osteoarthritis of knee joint.Methods　Two hundred and forty outpatients and inpatients were randomly divided into three groups, with 80 cases in each group. Group A was treated with ibuprofen sustained release capsules; Group B was injected sodium hyaluronate into knee joint cavity; Group C was injected with Sodium hyaluronate in combination with acupuncture and massage treatment. The patients, knee flexion and extension activity limp, propping up, instability, pain swelling, climbing stairs up and crouch were observed after a follow-up period of 6 months to 2 years.Results　According to Lyshlom knee functional scores, All of the group had a certain extent improvement in knee joints after treatment（P<0.05）. Group C improved better than Group A and group B（P<0.05）.Conclusion　Intra-articular injection of sodium hyaluronate in combination with acupuncture and massage is effective in the treatment of osteoarthritis. It can significantly reduce the local symptoms of knee joint, and increase joint activity and restore the knee joint function.]]> 2017/7/4 9:03:44 CHEN Yong, LU Hua-quan, DU YI-xia, et al 379true ［Abstract］　Objective　To explore the expression of EGR-1 in the prevention and treatment of liver fibrosis cholelithiasis in rabbit model by using Dahuanglingxian granule.Methods　The rabbit model of chronic liver fibrosis was established by intra-peritoneal injection of CCl₄^- olive oil, and given high-calorie diet and high-cholesterol diet; After the rabbit model was successfully established, the treatment group was treated with Dahuanglingxian granules 0.4 g/（kg·d）, and the other groups were on normal diet. The liver tissues of all the rabbits were tested by PCR and immunochemistry staining 12 weeks after the treatment.Results　（1）Immunochemistry staining: Compared with that in the model group, the positive expression of EGR-1 reduced obviously, and the less brown particles were observed in the cytoplasm and membrane of live cells in sinusoids and hepatic duct area in the treatment group, suggesting the positive effects of Dahuanglingxian granules on liver damage.（2）Results of RT-PCR: The mRNA levels of EGR-1 in the groups treated with Dahuanglingxian granules were decreased(P<0.05), indicating that this drug could effectively reduce the live damage by reducing the mRNA levels of EGR-1.Conclusion　Dahuanglingxian granules can effectively prevent the formation of cholelithiasis, reduce the mRNA and protein expression of EGR-1 in rabbit model of liver fibrosis cholelithiasis, and decrease the liver damage and improve the liver function.]]> 2017/7/10 17:13:59 WANG Bing, TANG Qian-li, WANG Yu, et al. 378true ［Abstract］　Objective　To investigate the corrlations between the expression of HER-2 and contrast-enhanced ultrasound(CEUS) and Acoustic radiation force impulse in breast cancers.Methods　A total of 108 patients with breast cancer were investigated. The HER-2 expression of the tumor was detected by immunohistochemistry after operation. The relationships between the expression of HER-2 and CEUS and ARFI were analyzed using enhancement features and time intensity curves(TIC) of CEUS, virtual tough imaging(VTI) and virtual tough quantification(VTQ) technology of ARFI, respectively.Results　(1)The differences of CEUS′s contrast media distribution, perforating branch blood flow, over range and perfusion defects between different levels of HER-2 expression were statistically significant（P<0.05）. The differences of TIC curve′s peak time(PT), rising slope(K) and area under curve(AUC) between different levels of HER-2 expression were statistically significant（P<0.05）.(2)The differences of VTQ performance between different levels of HER-2 expression were statistically significant(P<0.05).(3)The differences in comparision of VTI image characteristics, enhancement levels of contrast media and Peak intensity(PI) among different levels of HER-2 expression had no statistical significance（P>0.05）.Conclusion　CEUS and ARFI are related to the expression of HER-2 in breast cancer in some extent which is mainly shown in perfusion defects, perforating branch blood flow, peak time, rising slope and VTQ.]]> 2017/7/10 17:13:59 FANG Meng-Yuan, WANG Xiao-Yan, HE Yan, et al. 377true ［Abstract］　Objective　To study the risk of cardiovascular and cerebrovascular disease and intervention of atorvastatin in hypertension patients complicated with disorders of lipid metabolism.Methods　Fifty-four hypertension patients complicated with disorders of lipid metabolism in our hospital from December 2013 to December 2014 were collected as hypertension complicated with disorders of lipid metabolism group, other 54 patients with simple hypertension were taken as hypertension group. To study the intervention effect of atorvastatin, 54 hypertension patients complicated with disorders of lipid metabolism were randomly divided into the observation group and the control group, with 27 cases in each group. The observation group was treated with atorvastatin while the control group was treated with placebo for 8 weeks. The risk of cardiovascular and cerebrovascular disease was compared between the hypertension complicated with disorders of lipid metabolism group and the hypertension group. The systolic pressure, diastolic pressure, heart rate, total cholesterol(TC), triglyceride(TG), low density lipoprotein cholesterol(LDL-C), high density lipoprotein cholesterol(HDL-C), other blood lipid levels and adverse reactions were recorded before and after the intervention in the observation group and the control group.Results　The incidences of ventricular hypertrophy and coronary disease in the hypertension complicated with disorders of lipid metabolism group were significantly higher than those in the hypertension group(38.89% vs 20.37%, 33.33% vs 16.67%)(P<0.05). The systolic pressure and diastolic pressure in the observation group were significantly lower than those in the control group after the treatment(P<0.05). There was no significant difference in heart rate between the two groups after the treatment(P>0.05). TC, TG and LDL-C levels were significantly lower, and HDL-C level was significantly higher in the observation group than those in the control group after the treatment(P<0.05). There was no significant difference in adverse reaction rates between the two groups(P>0.05).Conclusion　Hypertension patients complicated with disorders of lipid metabolism have higher risk of cardiovascular and cerebrovascular disease. Atorvastatin can reduce the patients′ blood pressure effectively, regulate the blood lipid level with tolerable adverse effects in the treatment of hypertension complicated with disorders of lipid metabolism.]]> 2017/7/10 17:13:59 TAN Mei-hong, LIU Min-ling, ZHAO Ji-xuan 376true ［Abstract］　Objective　To investigate the correlations between visceral fat area and adipocytokines in prediabetic patients.Methods　Fifty-seven prediabetic patients were collected from outpatient visit and divided into 3 groups: the obesity group(n=14), the overweight group(n=22), and normal weight group(n=21). The levels of serum FINS, APN, TNF-α and IL-6 were detected. The homeostasis model assessment of insulin resistance(HOMA-IR) was used as a parameter to evaluate the degrees of insulin resistance.Total body fat percentage and visceral adipose tissue area（VATA） were assessed using dual emission X-ray absorptiometry.Results　(1)The levels of serum FINS, HOMA-IR and TNF-α were significantly higher in both the overweight group and the obesity group than in the normal weight group. The levels of APN were significantly lower in the overweight group and the obesity group than in the normal weight group. There were no significant differences in the levels of serum IL-6 among the three groups.(2)The total body fat percentage in the obesity group was significantly higher than that of the overweight group or the normal weight group. There were no differences in total body fat percentage between the overweight group and the normal weight group. Visceral adipose tissue area in the obesity group or the overweight group was significantly higher than that in the normal weight group.(3)APN was negatively correlated with FINS, WHR, HOMA-IR, VATA, total body fat percentage and TG, whereas TNF-α was positively correlated with FINS, WHR, HOMA-IR, VATA, TG and total body fat percentage.Conclusion　VATA is significantly increased in the overweight and obese prediabetic patients, which is relate to the decrease secretion of APN and the cause of disorder glucose metabolisms.]]> 2017/7/10 17:13:59 YU Jin-ming, HUANG Jian-hao, YU Yan-qing, et al. 375true ［Abstract］　Objective　To compare the differences of the upper airway sagittal diameters between the skeletal Class Ⅰ and Class Ⅱ sagittal facial type in male adults and to explore the relationship between the upper airway sagittal diameters and the skeletal sagittal facial type.Methods　Cephalograms of 60 male adults with Angle Class Ⅰ and Angle Class Ⅱ and average vertical facial type were collected and the patients were divided into two groups according to their different sagittal facial types, with 30 cases in each group. All the cephalograms were measured in upper airway sagittal diameters and the differences were compared between the two groups.Results　The sagittal diameters of palatopharyngeal, glossopharyngeal and laryngopharynx in Angle Class Ⅱ were narrower than Angle Class Ⅰ.Conclusion　There are some differences in upper airway sagittal diameters between the Angle Class Ⅰ and Angle Class Ⅱ, which means upper airway sagittal diameters may be associated with sagittal malocclusion.]]> 2017/7/10 17:13:59 TANG Qian, FANG Zhi-xin, ZHOU Yan, et al. 374true ［Abstract］　Objective　To observe the curative effect of desmopressin acetate combined with oxybutynin in the children with monosymptomatic nocturnal enuresis.Methods　One hundred and forty-eight children with monosymptomatic nocturnal enuresis were randomly divided into the observation group（n=76） and the control group(n=72). The two groups were given the basic treatment and oral conventional dose of desmopressin acetate, and the observation group added with Oxybutynin. The curative effect was observed 3 months after the treatment. The patients were followed up for 3 months to observe the recurrence after the drug withdrawal.Results　Fifty-two cases were markedly effective, 19 cases effective and 5 cases ineffective in the observation group while 30 cases were markedly effective, 26 cases effective and 16 cases ineffective in the control group. The curative effect of the observation group was significantly higher than that of the control group(93.4% vs 77.7%, P<0.01). After the drug withdrawal, the recurrence of the observation group was significantly lower than that of the control group(11.84% vs 25.00%, P<0.05).Conclusion　Desmopressin acetate combined with oxybutynin is more effective than desmopressin acetate in the treatment of children with monosymptomatic nocturnal enuresis.]]> 2017/7/10 17:13:59 ZHONG Qiong 373true ［Abstract］　Objective　To observe the clinical effect of immunoadsorption(IA) combined with immunosuppressive therapy on ANCA associated vasculitis.Methods　Four patients with ANCA-associated vasculitis were treated with HA280 immunoadsorben combined with hormones and cyclophosphamide. The changes of the levels of ANCA, BVAS, other biochemical indexes and clinical symtoms were compared before and after the treatment.Results　All of the patients′ clinical symptoms were improved after the treatment for 3 times, and ANCA turned negative in 1 case after the IA treatment, and ANCA turned negative in 2 cases 4 and 12 months after the IA treatment. ANCA maintained posotive in 1 case after a follow-up of 3 monthes. BVAs, hsCRP and ESR were markedly reduced afrer the IA treatment(P<0.05). Urinary protein and serum creatinine declined in 3 cases during the follow-up. Hypotension occured in 1 case, and transient platelets declined in 1 case. One patient died of aneurysm rupture bleeding after a follow-up of 3 months.Conclusion　DNA280 immunoadsorption combined with hormone and cyclophosphamide therapy can effectively reduce the level of ANCA, inhibit the activity of vasculitis, reduce proteinuria, and improve the renal function and clinical symptoms in the patients with ANCA associated vasculitis.]]> 2017/7/10 17:13:59 WANG Hao-yu, LIU Yuan-yuan, PENG Xiao-mei, et al. 372true ［Abstract］　Objective　To observe the clinical effect of Urinary Kallidinogenase in the treatment of large-scale acute cerebral infarction.Methods　Ninety-seven patients with large-scale acute cerebral infarction were randomly divided into two groups:the treatment group(n=50) and the control group(n=47). Both of the two groups were treated with a basic therapy, and the treatment group was administrated additional intravenous injection of Urinary Kallidinogenase 0.15 PNAU per day for 21 days. The clinical efficacy, the mortality rate, and the cerebral infarction area and NIHSS(the National Institutes of Health stroke scale, NIHSS) were compared between the two groups.Results　（1）In the treatment group,1 case missed, 3 cases died, and the mortality rate was 6.0%. In the control group, 10 cases died, and the mortality rate was 21.3%. There was a significant difference in the mortality rate between the two groups(P<0.05). （2）There was no significant difference in the change of the infarction area between two groups before the treatment(P>0.05). After the treatment, the infarction areas shrank in both of the two groups compared with those before the treatment, and these improved more obviously in the treatment group than those in the control group(P<0.05). （3）There were no significant differences in the scores of NIHSS between the groups before and 21 days after the treatment(P>0.05). One case occurred a sudden drop in blood pressure because of the combining use of ACEI class of antihypertensive drug, whose symptoms were improved by withdrawing the drug and symptomatic treatment. Except for this case, no obvious adverse reactions occurred in the treatment group.Conclusion　Urinary Kallidinogenase can effectively reduce the mortality rate and significantly decrease the cerebellar infarction volume in the treatment of large-scale acute cerebral infarction, but it can not obviously improve NIHSS within 21 days after the treatment. The long-term curative effect of Urinary Kallidinogenase remains to be studied.]]> 2017/7/10 17:13:59 MA Li-qin, WEN De-shu, XIE Yao-yi, et al. 371true ［Abstract］　Objective　To investigate the outcome of Helicobacter pylori(Hp) infection and the eradication of Hp after advanced gastric cancer surgery(Billroth Ⅱ).Methods　One hundred and three patients were performed radical distal gastrectomy whose Hp infections were pathologically conformed by endoscopic biopsy. ¹³C urea breath test(UBT) was used to diagnose Hp infection. Gastroscopy was used to observe anastomotic and residual stomach tissues. The existences of anastomotic inflammation, erosion and ulcer, chronic gastritis and other pathological changes including erosion and gastric intestinal metaplasia were observed. Sixty samples with Hp infection 4 weeks after the surgery were divided into the control group(n=30) and the experimental group （n=30）. Both of the two groups were given strict clinical observation and the experimental group received the additional medical eradication of Hp. Gastroscopy and CT were conducted every 6 months in all cases during a follow-up of 3 years. Metastasis and recurrence of tumors were also checked.Results　Of the 103 cases with radical distal gastrectomy, 76 cases suffered from Hp infection(73.3%) before the operation, 60 cases suffered from Hp infection(58.3%) four weeks after the operation, 43 cases suffered from Hp infection(41.7%) six months after the operation; 12 cases had anastomotic ulcer(11.6%) and 82 cases had anastomotic inflammation(80.0%) six months after the operation. The eradicate rate of Hp of the experimental group was significant higher than that of the control group(P<0.05); The residual gastritis of the experimental group was significantly lower than that of the control group(P<0.05), and anastomotic ulcers of the experimental group were lower than those of the control group, but without a significant difference(P>0.05). There was no significant difference in the cancer recurrence rate of residual stomach between the experimental group and the control group(P>0.05). There was no significant difference in the incidence rate of residual stomach cancer between Hp persistent positive and negative(P>0.05).Conclusion　Hp infection rate of residual stomach is decreased after advanced gastric cancer radical surgery. Anti-Hp therapy can improve the clinical manifestations in Hp positive patients after advanced gastric cancer radical surgery.]]> 2017/7/10 17:15:23 ZHONG Xiao-gang, WANG Xiao-tong, YIN Wu, et al. 370true ［Abstract］　Objective　To construct a 3-D finite element model（FEM） for lingual bracket system used in the retraction of six upper anterior teeth, and to lay the foundation for further biomechanical study of the retraction of upper anterior teeth in lingual orthodontic treatment.Methods　A 3-D FEM including the maxilla, alveolar bone, periodontal ligament, dentition(removal of the two first premolars), lingual brackets, arch wire with hooks(with different length) and micro-implants was constructed through a series of specialized software running based on a volunteer′s CT scan data, lingual brackets and other real items.Results　5 entire 3-D FEMs for lingual appliance with the hook lengths of 2 mm, 4 mm, 6 mm, 8 mm and 10 mm were constructed respectively, and each had 42 volume models.Conclusion　The originated 3-D FEMs for lingual appliance have higher geometrical similarity and resemblance of clinical situation, and can set the stage for further biomechanical research during the en-masse retraction of upper anterior teeth.]]> 2017/7/10 17:15:23 LIU Dai-bin, FANG Zhi-xin, ZHOU Yan, et al. 369true ［Abstract］　Objective　To compare the improving effects of vascular interventional therapy and simple medication in pressure-control level and cardiac function in the patients with atherosclerotic renal artery stenosis(ARAS).Methods　Eight-seven patients with ARAS in our hospital between January 2012 and July 2015 were selected as the study subjects. According to the principle of voluntrary, they were divided into the intervention group(n=45) and the simple medication group(n=42). SBP, DBP, Cr, cardiac function grading, LVEDD, LVEF, the curative rate of hypertension and the improvement rate of renal function were compared between the two groups before and after the treatment.Results　The differences in SBP, DBP, Cr, cardiac function grading, LVEDD and LVEF between the two group before the treatment were not significant(P>0.05). After the treatment, SBP and DBP in the two groups decreased and the differences before and after the treatment were significant(P<0.05). However, after the treatment, the differences between the two groups were not significant(P>0.05). Difference in Cr before and after the treatment was not significant(P>0.05). After the treatment, cardiac function grading and LVEDD in the intervention group were reduced to(2.2±0.4) and (52.5±4.6) mm, respectively which were significantly lower than those before the treatment and those in the simple medication group after the treatment; LVEF increased to (53.3±4.1)% which was higher than that before the treatment and that in the simple medication group after the treatment(P<0.05). The differences in the curative rate of hypertension and improvement rate of renal function between the two groups were not significant (P>0.05).Conclusion　Compared with simple medication, interventional therapy has no obvious advantages in the control of blood pressure and the improvement of renal function, however it can improve cardiac function better and reduce adverse cardiovascular and cerebrovascular events and prevent cardiovascular complications.]]> 2017/7/10 17:15:23 TANG Liang-yu, WANG Shu-yuan, TAN Shao-ling, et al. 368true ［Abstract］　Objective　To explore the correlation between pathological changes of pericancer tissues and posthepatectomy liver failure for hepatocellular carcinoma in the patients with Child-Pugh A.Methods　The data of 93 patients with Child-Pugh A who underwent liver resection for HCC in our hospital were retrospectively analyzed. The correlation between pathological changes of pericancer tissues and posthepatectomy liver failure and its correlation with serum liver biochemical indexes of preoperation and postoperation were evaluated.Results　There were significant differences in the pathological indicators and Knodell scores of pericancer tissues between 18 patients with liver failure and the other patients with no liver failure(P<0.001), and there were significant differences in the levels of preoperative serum prealbumin, γ-GT and ALP between the patients with liver failure and those with no liver failure(P=0.010, 0.000, 0.002). The Knodell scores of pericancer tissues and preoperative serum prealbumin, γ-GT and ALP were correlated(P=0.011, 0.001, 0.026) and they were also correlated with the serum γ-GT and ALP 7 days after the operation(P=0.004, 0.015).Conclusion　The degree of pathological damage in pericancer tissues,the high levels of preoperative serum γ-GT and ALP and the low level of prealbumin are closely correlated with the liver failure after hepatectomy.]]> 2017/7/10 17:15:23 YIN Wu, YANG Jian-rong, TANG Yun-tian, et al. 367true ［Abstract］　Objective　To explore the value of quantitative defection of cancer gene E6/E7 mRNA combined with thinprep cytology test(TCT) in the patients with cervical lesions.Methods　The data of 647 patients with cervical lesions in our hospital from May 2010 to June 2014 were retrospectively analyzed. Their cancer genes E6/E7 mRNA were quantitatively detected and these results combined with the results of TCT detection and the clinical pathological tests were compare between them.Results　ASCUS,LSIL, NSIL, and cervical cancer by quantitative detection of E6/E7 mRNA combined with TCT detection, went along with the severity levels of pathological classification from inflammation, CINⅠ,Ⅱ to CINⅢ, with significant differences among the groups(P<0.05).Conclusion　Quantitative detection of Oncogene E6/E7 mRNA combined with TCT may be used as for classifying cervical lesions of cervical cancer and early screening.]]> 2017/7/10 17:15:23 PAN Qi-wen, LI Jian-xiang, BAN Ting 366true ［Abstract］　Objective　To investigate the effects of acupuncture and exercise therapy on the patients with neurosurgical coma.Methods　 Seventy-four patients with neurosurgical coma in our hospital from March 2014 to August 2015 were randomly divided into the control group(n=37) and the observation group(n=37). Both of the two groups received the conventional therapy of Western medicine and nursing. The observation group received additional exercise plus acupuncture to awake from coma. The therapeutic effect and awaking rate were compared between the two groups.Results　The total efficiency of the observation group was significantly higher than that of the control group(83.78% vs 62.16%) 3 months after the treatment(P<0.05). The awaking rate of the observation group was significantly higher than that of the control group(78.38% vs 54.05%). The average awaking time of the observation group was shorter than that of the control group［(15.22±5.43) vs (28.92±6.68)d］ (P<0.05).Conclusion　Early acupuncture combined with physical therapy is effective to improve brain function and has better awaking action, in the patients with neurosurgical coma.]]> 2017/7/10 17:15:23 LU Jian-hua 365true ［Abstract］　Objective　To study the clinical effct of ritodrine hydrochloride combined with progestin capsule on the treatment of aura preterm birth.Methods　One hundred and eight patients in our hospital from Augest 2013 to February 2015 with aura preterm birth were colleted as the study subjects and were divided into the experimental group(n=54) and the control group(n=54). The control group was treated with ritodrine hydrochloride and the experimental group was given ritodrine hydrochloride combined with progesterone capsules. The spuc success rate, drug onset time,hospitalization time, adverse reactions, neonatal survival rate, birth weightand Apgar scores of the neonates where were compared between the two groups.Results　There was no statistically significant difference between the experimental group and control group in the spuc success rate(98.15% vs 94.44%). The hospital stay of the experimental group was significantly shorter than that of the control group(P<0.05), and the birth weight and the Apgar scores were better than those of the control group（P<0.05）. All the neonates survived in the two groups.Conclusion　Ritodrine hydrochoride combined with progestin is better than ritodrine in the treatment of aura preterm birth.]]> 2017/7/10 17:15:23 HUANG Dan-dan 364true ［Abstract］　Objective　To observe the clinical effect of Yinchenhao decoction with vitamin C on preventing neonatal hemolytic disease in pregnant women with a high titer of IgG antibody.Methods　One hundred and thirty-two pregnant women with blood type O and IgG antibody>1∶64 were randomly divided into the treatment group and the control group, with 66 cases in each group. The control group was given the traditional treatment of vitamin C. The treatment group received vitamin C combined with Yinchenhai decoction. The clinical effect was compared between the two groups.Results　The therapeutic effect of IgG antibody titer in the treatment group was significantly better than that in the control group(86.36% vs 58.09%), (P<0.05). The neonatal jaundice, total bilirubin content and the incidence of severe jaundice were significantly lower in the treatment group than those in the control group(3.70% vs 25.42%), (P<0.05).Conclusion　Yinchenhao decoction combined with vitamin C can reduce maternal IgG antibody titer and the incidence of the neonatal hemolytic disease and thus is safe and effective on preventing neonatal hemolytic disease in the pregnant women with a high titer of IgG antibody.]]> 2017/7/10 17:15:23 LIANG Jie 363true ［Abstract］　Objective　To investigate the prediction value of N-terminal pro-brain natriuretic protein(NT-proBNP) for the mortality of the inpatients with acute exacerbation of chronic obstructive pulmonary disease(AECOPD).Methods　The clinic data of 193 AECOPD patients whose values of NT-proBNP had been examined were collected. All the patients were divided into the survival group and the death group according to the survival status at the time of discharge from the hospital. A receiver-operator-curve(ROC) analysis was used to determine the level of NT-proBNP that discriminated survivors from non-survivors. Univariate analysis and multiple logistic regression analyses were used to identify the risk factors of in-hospital mortality.Results　During the patients′ hospitalization, 20 cases died and 173 cases survived. The death group had a higher level of NT-proBNP than the survival group ［(7 040±10 150）pg/ml vs （2 147±4 774）pg/ml, P<0.001］. The area under the ROC curve for the NT-proBNP prediction of death was 0.804(95%CI: 0.730～0.879). The value of NT-proBNP discriminating survivors from non-survivors was 1 249.5 pg/ml, with a sensitivity being 80.0% and a specitity being 71.1%. Univariate analysis showed that blood urea nitrogen(BUN )>7.14 mmol/L, blood cell （WBC）≥10×10⁹/L, NT-proBNP≥1 249.5 pg/ml, the propotion of neutrophils(N%)≥80%, body mass index(BMI) and the comorbidy of chronic heart failure were the risk factors of in-hospital mortality. Multiple logistic regression analysis showed that N% >80%(OR=21.47.95%CI=2.42～190.40), NT-proBNP>1 249.5 pg/ml(OR=7.68, 95%CI=1.72～34.38) and heart failure(OR=3.96, 95%CI=1.02～15.40) were the independent risk factors of in-hospital mortality of AECOPD, and that increasing BMI was a protective factor of in-hospital mortality of AECOPD.Conclusion　The elevated level of NT-proBNP is a strongly independent risk factor of mortality in the AECOPD inpatients.]]> 2017/9/26 8:05:56 HU Guo-ping, LI Yu-qun, WU Ze-long, et al. 362true ［Abstract］　Objective　To investigate the effects of Duhuojisheng decoction combined with extracorporeal shock wave on the expressions of Interleukin-1β(IL-1β), Superoxide Dismutase(SOD) and Matrix Metalloproteinase-3(MMP-3) in the treatment of knee osteoarthritis(KOA).Methods　300 KOA outpatients(300 knees) were collected as the study subjects from our hospital during September 2013 and March 2016. All the patients were randomly divided into three groups. The Traditional Chinese Medicine group(n=100) was treated with Duhuojisheng decoction. The extracorporeal shock wave group(n=100) was treated with extracorporeal shock wave, and the combined therapy group(n=100) was treated with Duhuojisheng decoction combined with extracorporeal shock wave. The expressions of IL-1β, SOD and MMP-3 in the joint fluid of the three groups were detected respectively at the time points of pretreatment and 2, 4, 6, 12, 24 weeks after the treatment.Results　(1)Compared with those within groups before treatment, there were significant differences in the expressions of IL-1β, SOD and MMP-3 2, 4, 6, 12 and 24 weeks after treatment(P<0.05), which showed that all groups can reduce the expressions of IL-1β and MMP-3 and increase the expression of SOD. (2)There were no significant differences in the expressions of IL-1β, SOD and MMP-3 at different time points between groups befor treatment(P>0.05). There were no significant differences in the levels of IL-1β, SOD and MMP-3 between the Traditional Chinese Medicine group and the extracorporeal shock wave group at the time points of 2, 4, 6, 12 and 24 weeks. There were significant differences between the combined therapy group and the Traditional Chinese Medicine group or the extracorporeal shock wave group in the levels of IL-1β, SOD and MMP-3 at the time points of 2, 4, 6, 12 and 24 weeks(P<0.05). These results showed that the Traditional Chinese Medicine group and the extracorporeal shock wave group had the same effects on down-regulation of the expression of IL-1β, MMP-3 and up-regulation of the expression of SOD, however the combined therapy group had better effects than the other two groups.Conclusion　Duhuojisheng decoction combined with extracorporeal shock wave can down-regulate the expressions of IL-1β and MMP-3, and up-regulate the expressions of SOD in the knee joint fluid for the patients with KOA.]]> 2017/9/26 8:05:56 JIN Xian-yue, LI Hong-yu, TIAN Wen, et al. 361true ［Abstract］　Objective　To investigate the effect of cardiac resynchronization therapy(CRT) on left ventricular remodeling in patients with chronic congestive heart failure(CHF).Methods　21 CHF patients who were in accordance with the guidelines for class Ⅰ indication were enrolled, of whom 13 cases suffered from dilated cardiomyopathy and 8 cases suffered from ischemic cardiomyopathy. Left ventricular ejection fraction(LVEF), left ventricular end diastolic diameter(LVDD), left ventricular end systolic diameter(LVDS), left ventricular end diastolic volume(LVEDV), left ventricular end systolic volume（LVESV） and left atrium diameter（LAD） were observed 6 and 12 months after the CRT.Results　Compared with those before the CRT, the LVEF was increased significantly and the LVDD, LVDS, LVEDV, LVESV and LAD were decreased significantly 6 and 12 months after the CRT（P<0.05）. There were no significant differences in all the above parameters between 6 months and 12 months after the CRT（P>0.05）.Conclusion　CRT can significantly reverse left venticular remodeling and increase LVEF in the patients with CHF 6 months after the operation.]]> 2017/9/26 8:05:56 LI Chi, HU Chang-xing, LI Qing-kuan, et al. 360true ［Abstract］　Objective　To investigate the characteristics of pathogens in patients with complicated intra-abdominal infections in Intensive Care Unit(ICU).Methods　The clinical data of 82 ICU patients with complicated intra-abdominal infections were retrospectively analyzed and the distribution of pathogenic bacteria was described.Results　(1) Gram-negative bacteria were found in 58 cases(52.7%), fungi in 32 cases(29.1%) and Gram-positive bacteria in 20 cases(18.2%).(2)The proportion of fungal infections was higher in the hospital-acquired group than that in the community-acquired group（P<0.05）.（3）Compared with the survival group, the death group had higher fungal infections and bactera mixed infections（P<0.05）, and more cases of initial inappropriate antibiotic therapy(P<0.05).Conclusion　The two most frequently isolated pathogens in the patients with complicated intra-abdominal infections are Gram-negative bacteria and fungi. Laparotomy is a risk factor for the incidence of complications such as fungal infections(mostly result from Candia albicans) and mixed infections, which affects the prognosis.]]> 2017/9/26 8:05:56 LIU Yuan-bo, ZHANG Hong-bin, ZENG Ming-hao, et al. 359true ［Abstract］　Objective　To explore the effect of TDP(Te-ding Dian-ci-bo Pu) plus low-molecular-weight heparins calcium on preventing phlebitis in the patients with malignant tumors after peripherally inserted central catheter(PICC).Methods　360 patients with malignant tumors who accepted PICC were randomly divided into the experience group(n=180) and the control group(n=180). The experience group was treated with subcutaneous injection of low-molecular-weight heparins calcium(4 100 IU daily for 7 days) plus TDP(24 hours after catheterization, the above catheter puncture site and the clavicle area were irradiated for 30 min, twice daily for 6 days). The control group was treated with low-molecular-weight heparins calcium. The incidence of phlebitis was compared between the two groups within 2 weeks.Results　The incidence of phlebitis in the experience group(7.78%) was significantly lower than that in the control group(20.56%, P=0. 001).Conclusion　TDP plus low-molecular-weight heparins calcium can reduce the incidence of phlebitis in the patients with malignant tumors after PICC.]]> 2017/9/26 8:05:56 LAN Hai-yan, FENG Guo-sheng, HAO Yan-rong, et al. 358true ［Abstract］　Objective　To investigate the relationship between female pelvic organ prolapse and stress urinary incontinence.Methods　 The eligible participants were randomly selected from the Maternal and Child Health care Hospitals in Liuzhou region by cluster sampling. They were surveyed by questionnaire and thereafter received gynecological examination(POP-Q). The relationship between pelvic organ prolapse and stress urinary incontinence was statistically analyzed.Results　The prevalence of stress urinary incontinence among the patients with pelvic organ prolapse was 51.3%. The prevalence of stress urinary incontinence was 22.1% in the patients with anterior vaginal wall prolapse, 18.5% in those with uterine prolapse and 18.2% in those with posterior vaginal wall prolapse. There were significant differences in the incidences of stress urinary incontinence among varying degrees of uterine prolapse（χ²=147.700, P<0.01）.With the increasing degrees of uterine prolapse, the incidences of stress urinary incontinence increased（χ²=114.222, P<0.01）. However, there were no significant differences in the incidences of stress urinary incontinence among varying degrees of posterior vaginal prolapse.Conclusion　Except for the posterior vaginal wall prolapse, the more serious conditions, the higher is the incidence of complicated stress urinary incontinence.]]> 2017/9/26 8:05:56 YING Ting, WANG Xin-dan, TAO Hua 357true ［Abstract］　Objective　To investigate the curative effect of repairing bile duct stricture using pedicled cholecyst graft.Methods　A total of 110 patients with cholangiocarcinoma were enrolled in this study. They were randomly divided into the observation group(n=55) and the control group(n=55). The control group was treated with cholangio lithotomy and bile Roux-en-Y anastomosis. The observation group received the same treatment as the control group plus the repair of bile duct stricture using pedicled cholecyst graft, T-tube insertion and bile intestinal drainage.Results　After a follow-up of one year, the recurrence rate of bile duct stricture in the observation group was significantly lower than that in the control group, and the recurrence rate of cholelithiasie in the observation group was lower than that in the control group. The incidence of complications(including upper gastrointestinal ulcer, reflux cholangitis and biliary tract infection) in the observation group was lower than that in the control group(P<0.05).Conclusion　Pedicled cholecyst graft is safe and effective for the patients with bile duct stenosis.]]> 2017/9/26 8:05:56 ZHENG Da-wu, LUO Yong-xiang, LAO Jing-mao, et al. 356true ［Abstract］　Objective　To investigate the protective effect of Sanguis Draconis flavones（SDF）on myocardial cells in rats with myocardial ischemia-reperfusion injury(MIRI).Methods　40 SD rats were randomly divided into five groups: control group, sham group, MIRI group, sham+SDF group and MIRI+SDF group, with 8 cases in each group. The MIRI model was established by ligating left anterior descending coronary artery for 30 minutes and reperfusing for 2 hours. The drugs were administered one week before operation. The levels of serum creatine kinase(CK), creatine kinase isoenzyme MB(CK-MB) and lactic dehydrogenase(LDH) were measured by colorimetric assay. The area of myocardial necrosis was assessed by triphenyltetrazolium chloride(TTC) staining, and the apoptosis rate of cardiomyocytes was detected by terminal-deoxynucleoitidyl transferase mediated nick end labeling(TUNEL).Results　Compared with those in the control group, sham group and the sham+SDF group, the levels of CK, LDH and CK-MB in the MIRI group and the MIRI+SDF group were significantly higher(P<0.05), while compared with those in the MIRI group, the levels of CK, LDH and CK-MB in the MIRI+SDF group were significantly lower(P<0.05). The area of myocardial infarction in the MIRI+SDF group was significantly smaller than that in the MIRI group(P<0.05), and the apoptotic rate of myocardial cells in the MIRI+SDF group was significantly smaller than that in the MIRI group(P<0.05).Conclusion　Sanguis Draconis flavones can effectively protect the myocardial cells of rats with myocardial ischemia-reperfusion injury.]]> 2017/9/26 8:05:56 LIANG Li-mei, FENG Xiang-ling, LIU Yan, et al. 355true ［Abstract］　Objective　To analyse the clinical long-term effect of femtosecond laser surgery on refractive error.Methods　200 eyes were included in the femtosecond laser group and the cornea microkeratome group respectively. The eyesight, refractive diopter, high order aberration and contrast sensitivity were compared between the two groups six months after surgery.Results　Six months after surgery, there was no significant difference in efficacy index between the cornea microkeratome group(after UCVA/before BCVA) （0.98±0.20） and the femtosecond laser group（1.02±0.22）（P>0.05）. There were no significant differences in equivalent sphere lens between the cornea microkeratome group（0.44±0.22）D, and the femtosecond laser group（0.14±0.22）D（P>0.05）. For the changes of aberration before and after surgery, total aberration was 0.50, spherical aberration was 0.56, coma was 0.16 and clover was 0.12 in the femtosecond laser group and in the cornea microkeratome group, and the corresponding data were 0.43, 0.48, 0.19, and 0.09, respectively, without significant differences between the two groups. There was no significant difference in contrast sensitiveness between the two groups.Conclusion　Both of the clinical long-term effects of femtosecond laser surgery and cornea microkeratome LASIK surgery are favorable on correcting refractire error.]]> 2017/8/29 17:08:47 CAI Jing- feng, RUI Yan- jun, MAO Kai- bo, et al. 354true ［Abstract］　Objective　To investigate the effect of modified Simiao powder assisted arthroscopic debridement on treating chronic gonarthromeningitis.Methods　101 patients with chronic knee osteoarthritis who were treated in our hospital from June 2014 to May 2016 were collected. They were divided into the observation group(n=51) and the control group(n=50) by drawing lots. The control group was treated with arthroscopic debridement. The observation group was treated with modified Simiao powder after arthroscopy debridement. The degree of pain, the scores of joint swelling before surgery and one month after surgery, and the knee function before surgery and three months after surgery were compared between the two groups. The adverse reactions during treatment were recorded and the clinical efficacy at postoperative three months were compared between the two groups.Results　Compared with that in the control group, the clinical curative effect in the observation group increased significantly(P<0.05); Compared with those in the control group, at postoperative one month, the VAS scores in the observation group reduced significantly, and the scores of joint swelling improved significantly(P<0.05); Compared with those in the control group at postoperative three months, the Lysholm scores in the observation group increased significantly(P<0.05). There was no adverse reaction in the control group. There were 7 patients with stomach discomfort which was improved after symptomatic treatment in the observation group during the treatment period. There were significant differences in the adverse drug reactions between the two groups.Conclusion　The modified Simiao powder assisted arthroscopic debridement can effectively reduce the postoperative pain, swelling and other symptoms, and promote the recovery of knee joint function, and improve the clinical curative effect in the treatment of chronic gonarthromeningitis.]]> 2017/8/29 17:08:47 HUANG Xiao-han, YANG Pei-pei 353true ［Abstract］　Objective　To compare the treatment effects on intracranial aneurysms between craniotomy and endovascular embolization.Methods　We retrospectively analyzed the data on consecutive cerebral aneurysm patients treated in our department between January 2013 and January 2016. 48 patients were enrolled in the microsurgical clipping group and 51 patients in the intravascular embolization group. The occurrences of brain infraction and rebleeding were analyzed. The glasgow outcome scale（GOS） was assessed 1 month after the treatment.Results　In the intravascular embolization group, complete occlusion was observed in 40 patients, subtotal occlusion in 6 patients, and incomplete embolizationin in 2 patients. The good outcome rate of the embolization group(GOS≥4 GOS)(96.07%) was higher than that of the microsurgical clipping group(P<0.05). The occurrences of infraction and rebleeding in the embolization group were lower than those in the microsurgical clipping group(P<0.05). There were no significant differenes in the recurrence rates between the two groups（P>0.05）.Conclusion　Endovascular embolization is a safe alternative treatment method for cerebral aneurysms.]]> 2017/8/29 17:08:47 ZHONG Shu, XIAO Quan, PANG Gang, et al. 352true ［Abstract］　Objective　To discuss the causes of the cross-matching incompatibility and the relevant managing methods.Methods　We retrospectively analyzed the data of 170 patients with cross-matching incompatibility who needed blood transfusion from the blood bank in our hospital from January 2014 to December 2016, and summarized the causes of cross-matching incompatibility before blood transfusion.Results　In the 170 patients with cross-matching incompatibility, there were 88 cases with irregular antibody(51.76%), 62 cases with auto-antibodies(36.47%), 14 cases with ABO subtype(8.24%), 4 cases with protein aggregation(2.35%) and 2 cases with drug reason(1.18%). In the cases with irregular antibodies, there were 32 cases with Rh system(including 3 cases with joint antibodies), 19 cases with MNSs system, 6 cases with Lewis system, 1 case with Kidd, 1 case with Diego system, 7 cases with multiple antibodies. 16 cases failed to detect the antibodies, and 6 cases with unidentified antibodies.Conclusion　The main cause of cross-matching incompatibility is the same irregular antibodies, followed by autoantibodies. Clinical blood transfusion schemes should be determined by the types and specific causes of the cross-matching incompatibility.]]> 2017/8/29 17:08:47 WEI Liang-ying, LI Hai-lan, LUO Rui-xian, et al. 351true ［Abstract］　Objective　To assess the variation of Th17 cells in peripheral blood, tumor and tumor-adjacent tissues in the patients with non-small-cell lung cancer(NSCLC) and to evaluate the correlation between their expressions and the clinicopathological features and to explore the probable mechanisms of its elevation.Methods　Thirty NSCLC patients and twenty healthy controls were studied. All the samples of peripheral blood and tumor and tumor-adjacent tissues from 30 NSCLC patients were obtained. The proportions of Th17 cells in the peripheral blood, tumor tissues and tumor-adjacent tissues were evaluated by flow cytometry. The serum concentrations of IL-6, IL-17, IL-23 and TGF-β1 were measured by enzyme-linked immunosorbent assay(ELISA). The mRNA expressions of RORc and IL-17 in the peripheral blood, tumor tissues and tumor-adjacent tissues were examined by real-time quantitative polymerase chain reaction(QPCR).Results　(1)The proportion of Th17 cells in the peripheral blood was significantly higher in the NSCLC patients (3.81±0.63)% than that in the healthy controls (0.73±0.22)%(P<0.001). The proportion of Th17 cells in the tumor tissues (5.07±0.28)% was significantly higher than that in their matched peripheral blood (3.75±0.70)% or tumor-adjacent tissues (3.00±0.25)%(P<0.001). (2)The levels of RORc mRNA （3.91±0.48） and IL-17 mRNA （68.91±6.31） in the peripheral blood were significantly higher in the NSCLC patients than those in the healthy controls(P<0.001). The levels of RORc mRNA （4.94±0.31） and IL-17 mRNA （101.61±9.42） in the tumor tissues were significantly higher than those in their matched peripheral blood ［(3.91±0.48),(68.34±6.04)］ or tumor-adjacent tissues［(2.35±0.21),(46.02±4.11)］(P< 0.001). (3)The proportion of Th17 cells and the levels of IL-17 in peripheral blood and in tumor-adjacent tissues in the NSCLC patients with stage Ⅲ or Ⅳ were significantly higher than those in the patients with stage Ⅰ or Ⅱ(P<0.05). （4）The serum levels of IL-6, IL-17, IL-23 and TGF-β1 in the NSCLC patients were significantly higher than those in the healthy controls(P< 0.05). The levels of IL-6, IL-17, IL-23 and TGF-β1 in the tumor tissues were significantly higher than those in their matched peripheral blood and tumor-adjacent tissues(P<0.001).（5）The number of Th17 cells was significantly correlated with the levels of IL-6, IL-23 and TGF-β1 in the peripheral blood and tumor tissues from the NSCLC patients(P<0.01).Conclusion　There are up-regulated expressions of Th17 in the peripheral blood, tumor and tumor-adjacent tissues from the NSCLC patients. With the progression of the disease, high invasion and migration into the circulation may exist. The elevated levels of IL-6, IL-23 and TGF-β may be related to the above effects.]]> 2017/8/29 17:08:47 WEI Yu-ping, DUAN Min-chao, HAN Wei, et al. 350true ［Abstract］　Objective　To investigate the clinical effect of aerosol inhalation of Tanreqing injection on the patients with mild bronchial asthma.Methods　60 patients with mild bronchial asthma admitted to our hospital from June 2015 to June 2016 were selected and were divided into three groups according to the different treatment regimens. The control group(n=30) received conventional expectant treatments of anti-infection, glucocorticoid, aminophylline and oxygen inhalation. The observation group(n=30) received the same treatment as the control group plus aerosol inhalation of Tanreqing injection. Both of the two groups were treated for 4 weeks and the levels of endothelin(ET) and nitric oxide(No), and the clinical efficacy were observed.Results　After the treatment, the conditions of the patients were improved in both of the two groups. 18 cases were makkedly effective, 10 cases effective and 2 cases ineffective in the control group, while 11 cases were markedly effective, 11 cases effective, and 8 cases ineffective in the control group，with a better clinical effect in the observation group(P<0.05). There were no significant differences in the levels of ET and NO between the two groups before the treatment. After the treatment, the levels of serum ET and NO decreased more significantly in the observation group than those in the control group. The disappearance time of cough, dyspnea and wheezing in the observation group was significantly shorter than that in the control group(P<0.05).Conclusion　Aerosol inhalation of Tanreqing injection can effectively improve the levels of serum ET and NO, relieve the clinical symptoms, and improve the therapeutic effect in the patients with mild bronchial asthma.]]> 2017/8/29 17:08:47 MENG Jian-feng 349true ［Abstract］　Objective　To observe helicobacter pylori(HP) infection in the patients with acute cerebral infarction, and to investigate its effects on glucose metabolism and blood lipid metabolism.Methods　The data of ninety-four cases in our hospital from April 2014 to November 2016 were retrospectively analyzed in whom 44 patients who suffered from acute cerebral infarction were taken as the observation group and 50 healthy examined people as the control group. The serum levels of fasting blood glucose(FBG) and plasma lipid including triglyceride(TG), total cholesterol(TC) and low density lipoprotein-cholesterol(LDL-C) were detected. At the same time, ¹⁴C-breath test was performed as well.Results　The infection rate of Hp in the observation group(70.45%) was significantly higher than that in control group（50.00%）(P<0.05). The serum levels of TG, TC and LDL-C in the Hp(+) cases were significantly higher than those in the Hp(-) cases in the observation group(P<0.05), but there was no significant difference in the level of FBG between the Hp(+) cases and the Hp(-) cases(P>0.05).Conclusion　The serum level of lipid increases with Hp infection, which may lead to acute cerebral infarction after HP infection.]]> 2017/8/29 17:08:47 PANG Jia-wu, LIN Chun-guang, TANG Wei-feng 348true ［Abstract］　Objective　To explore the expressions of TMPRSS4 mRNA in lung adenocarcinoma tissues and their correlations with lung adenocarcinoma.Methods　The expressions of TMPRSS4 mRNA in cancerous tissues of 41 patients with lung adenocarcinoma, and their matched adjacent tissues, and the expressions of lung tissues of 12 cases with benign pulmonary disease were detected using real-time fluorescent quantitative PCR.Results　The expressions of TMPRSS4 mRNA in lung adenocarcinoma tissues were higher than those in the matched adjacent tissues(P<0.01). The expressions of TMPRSS4 mRNA in the normal lung tissues were lower than those in the matched adjacent tissues（P<0.01）. There was significant correlation between the expression of TMPRSS4 mRNA and the lymph node metastasis, and TNM stage of lung adenocarcinoma(P<0.05). There was no significant correlation between the expression of TMPRSS4 mRNA and age, gender and the degree of pathological differentiation.Conclusion　TMPRSS4 expression in lung adenocarcinoma is closely related to the tumor occurrence and metastasis. Higher expression of TMPRSS4 mRNA indicates higher risk of tumor metastasis and the possibility of a worse prognosis in the patients with lung adenocarcinoma.]]> 2017/8/29 17:08:47 LI Shou-nan, WU Zheng-qiu, LU Qiang-chang, et al. 347true ［Abstract］　Objective　To observe the efficacy of ademetionine in treatment of hyperbilirubinemia in patients with posthepatitic cirrhosis.Methods　Sixty-two patients with posthepatitic cirrhosis in our hospital from May 2014 to November 2015 were randomly divided into the observation group(n=31) and the control group(n=31). The patients in the observation group received ademetionine 1.0 g+5%GS 250 ml,ivgtt,qd, and the patients in the control group were given Traditional Chinese Medicine Kuhuang injection 30 ml+5%GS 250 ml, ivgtt,qd. Both of the two groups received the conventional liver-protecting treatment. The clinical effects were observed 2 and 4 weeks after the treatment.Results　The levels of serum total bilirubin(TBIL),direct bilirubin(DBIL), alanine aminotransferase(ALT)and gamma-glutamyl transpeptidase(GGT) in the observation group were significantly lower than those in the control group(P<0.05) 2 weeks after the treatment, but there were no significant differences in the levels of alkaline phosphatase(ALP) and albumin(ALB) between the two groups(P>0.05). The observation group had lower levels of serum TBIL, DBIL, ALT, GGT and ALP(P<0.01), but higher ALB(P<0.05) than the control group four weeks after the treatment. The levels of TBIL, DBIL, GGT, and ALP decreased more greatly in the observation group than those in the control group(P<0.05).Conclusion　Ademetionine has the actions of eliminating jaundice and improving liver synthetic function and therefore is effective in the treatment of the patients with posthepaitic cirrhosis.]]> 2017/7/28 17:24:59 LIN Man, XIE Zhi-wei, SHI Hai-yan, et al. 346true ［Abstract］　Objective　To investigate the clinical application value of spiral CT three-dimensional reconstruction imaging in percutaneous nephrolithotomy.Methods　The data of 52 patients with renal stones(including bilateral stones in 6 cases) in our hospital From January 2015 to October 2016 were retrospectively analyzed. Spiral CT scanning and three-dimensional reconstruction imaging were performed before operation and percutaneous nephrolithotomy was performed under the guidance of ultrasound. The operation results were analyzed.Results　(1)52 cases(58 sides) were performed nephrolithotomy, among whom 46 cases(50 sides) were performed single channel operation, and 6 cases(8 sides) underwent dual channel operation. (2)45 cases(49 sides) received one-stage operation. 3 cases(5 sides) were performed second-stage operation. 4 cases(4 sides) were converted to open surgery. The time for operation was 15 min～6 h, with an average of (2.31±1.02)h. The stone clearance rate was 84.48%, and the average hospitalization stay was (12.83±3.24)days. (3)No complications such as bleeding and surrounding organs damage were found.Conclusion　The spiral CT three-dimensional reconstruction imaging has a high clinical application value in percutaneous nephrolithotomy.]]> 2017/7/28 17:24:59 HUANG Zi-Rong, WU Bing-Quan, LIU Zhao-hua, et al. 345true ［Abstract］　Objective　To investigate the correlation between vitamin D₃ and unexplained abortion immunology.Methods　36 patients with early unexplained missed abortion were selected as the observation group in our hospital from January 2015 to March 2016. Other 30 patients with normal maternal abortion were taken as the control group in our hospital during the same period of treatment as the observation group. The levels of serum vitamin D₃, IFN-γ and IL-4 were detected, and the correlation among them was analyzed.Results　The average level of vitamin D₃ in the observation group （12.91±2.61）nmol/L was significantly lower than that in the control group （20.03±3.82）nmol/L（P<0.05）. The level of IFN-γ was significantly higher while the level of IL-4 was significantly lower in the observation group than those in the control group（P<0.05）. Vitamin D₃ had a significant negative correlation with IFN-γ, and had a positive correlation with IL-4(P<0.05).Conclusion　The abnormal levels of vitamin D₃ may cause the abnormal levels of IFN-γ and IL-4, which results in the abnormal immune function and the occurrence of missed abortion.]]> 2017/7/28 17:24:59 SU Zhen-wen, JIANG Yan-hua, HUANG Hong-li 344true ［Abstract］　Objective　To assess the short-term outcomes of intensity-modulated radiation therapy(IMRT) combined with concurrent nedaplatin plus 5-flurouracil(NF regimen) or oxaliplatin plus 5-fluorouracil(OF regimen) in patients with local advanced nasopharyngeal carcinoma(NPC).Methods　40 patients with local advanced NPC were treated at the clinical cancer center of the People′s Hospital of Guangxi Zhuang Autonomous Region during February 2016 and May 2016, and their clinical data were retrospectively analyzed. Of the 40 patients, 20 cases(NF group) were treated with IMRT and concurrent NF chemotherapy, and the other 20 cases(OF group) were treated with IMRT and concurrent OF chemotherapy. The short-term outcomes and toxicities were compared between the two groups.Results　There was no significant difference in the clinical effect between the two groups 1 month after treatmeant(P>0.05). In the severe adverse reactions of grade 3, the NF group had a significantly higher rate of oral mucositis than the OF group(P<0.05), except for this, there were no significant differences in the incidences of other severe adverse reactions between the two groups, with all of the incidences being≤10%.Conclusion　The short-term clinical effect of IMRT plus concurrent NF regimen is equivalent to that of IMRT plus concurrent OF regimen. However, the latter regimen has less severe oral mucositis.]]> 2017/7/28 17:24:59 PANG Qiang, LU He-ming, CHEN Jia-xin, et al. 343true ［Abstract］　Objective　To investigate the clinical effect of Rouganyangxuerunjinlifafang on knee osteoarthritis.Methods　100 patients with knee osteoarthritis were selected from January 2015 to January 2017, and were randomly divided into the study group and the control group, with 50 cases in each group. The control group received conventional treatment of joint injection. The study group received the same treatment as the control group plus Rouganyangxuerunjinlifafang. The clinical effects, VAS and HSS scores were compared between the two groups.Results　The treatment efficiency of the study group(92%) was significantly higher than that of the control group(78%)(P<0.05). After treatment, VAS and HSS scores were improved in both of the two groups(P<0.05), but VAS score of the study group was significantly lower than that of the control group(P<0.05), and HSS score of the study group was significantly higher than that of the control group(P<0.05).Conclusion　Rouganyangxuerunjinlifafang has better clinical effect on knee osteoarthritis.]]> 2017/7/28 17:24:59 SUN Dong-ping 342true ［Abstract］　Objective　To investigate the value of high-frequency ultrasound in the diagnosis and treatment of the closed rupture of the long head of the biceps tendon(LHB).Methods　30 patients with clinically suspected LHB injury were examined by ultrasonography, and the ultrasonic results were compared with the results of intraoperative exploration, shoulder arthroscopy or MRI findings. After the operative treatment, high-frequency ultrasound examination was performed on the patients for three times.Results　The diagnostic sensitivity of ultrasound was 91.7%(22/24), the specificity was 83.3%(5/6), and the accuracy was 90%(27/30). During the wound healing process, the ultrasonic images changed with time.Conclusion　High-frequency ultrasound can accurately diagnose LHB fracture, which is helpful for the formulation of clinical treatment plan, and has important clinical value in the judgment of the wound healing effect.]]> 2017/7/28 17:24:59 YANG Liu, HUANG Yi-feng, OU Jing, et al. 341true ［Abstract］　Objective　To explore the effect of electronic module of nursing documents on improving nursing work efficiency and nursing service quality and nursing management. Methods　According to the requirements and the relevant content in “The Nursing Documents Writing Standards” issued by the National Health Planning Commission, combined with the condition of nursing staff, the nature of work and professional characteristics, the electronic module of nursing documents was designed and developed and the electronic module of nursing documents can be used in the whole hospital and the various specialized departments(wards). The electronic module of nursing documents was developed in January 2016 and was first tested in 3 departments, then popularized in all departments of the hospital in June 2016. The nursing work efficiency and nursing service quality were summarized and analyzed before and after the application of electronic module.Results　After the application of the electronic module, the work efficiency and quality of nursing documents writing in the nursing staff were improved, and the relevant people′s satisfaction to nursing work was higher than that before the application with a statistical significance(P<0.01).Conclusion　The contents and format of electronic module of nursing documents are complete, standard, scientific and normative. The electronic module of nursing documents has the advantages of convenient, quick and practical application, and can effectively improve the writing work efficiency and nursing service quality.]]> 2017/7/28 17:24:59 QIN Ai-fang, WU Jin-ju, QIN Zhao-ju, et al. 340true ［Abstract］　Objective　To explore the effect of Dahuanglingxian capsules on regulating the liver and kidney functions and the changes of their ultrastructural structures.Methods　80 New Zealand white rabbits were randomly divided into the normal group, the model group, the control group and the treatment group, with 20 cases in each group. The rabbits in the normal group were fed with conventional feed. The rabbits in the model and treatment groups were fed with calculi feed, and the rabbits in the control and the treatment groups were given intragastric administration with Dahuanglingxian capsules at the dose of 640 mg/kg/day for 8 weeks. The biochemical tests of venous blood, and the pathological examination of liver tissues by electron microscope were performed.Results　Compared with those in the normal group, the indexes of liver function in the model group were significantly increased, but compared with those in the model group, the indexes of liver function in the treatment group were significantly decreased(P<0.01). Liver pathological results showed that the structure of liver tissue was not changed obviously in the normal group. Liver fibrosis was obviously increased and narrow gap was formed. Part of the liver tissues presented lipoid degeneration or necrosis in the model group. Only slight liver fibrosis was found in the treatment group. The ultrastructural results of the liver tissues showed that liver cell nucleus were irregular and part of the nucleus presented pycnosis and apoptosis in the model group. Endoplasmic reticulum appeared in the forms of expansion and hyperplasia. Degranulation phenomenon of rough endoplasmic reticulum was found and mitochondria crests were fused with bubble edema. Fibroblasts presented spindle with lipid droplets and cytoplasm dissolved. In the treatment group nucleus were round, and the nuclear gaps were wider than those in the normal group. Lipid droplets were hardly found in the cytoplasm. Bubbles were still seen in the mitochondria and abundant organelles were observed in cytoplasm.Conclusion　Dahuanglingxian capsules can reduce liver fibrosis and liver function damage caused by cholelith disease, and repair the damaged liver cells and maintain the stable hepatocellular structures.]]> 2017/6/28 15:27:54 HU Si-qin, CHEN Lin, WANG Bing, et al 339true ［Abstract］　Objective　To explore the changes of central corneal thickness(CCT) and intraocular pressure(IOP) in the primary acute angle-closure glaucoma after combined trabeculectomy.Methods　48 patients who had been attacked by primary acute angle-closure glaucoma with only one eye attacked were collected as the research subjects.The attacked eyes were taken as the observation group, and the normal eyes as the control group. The observation group received a combined trabeculectomy. The changes of CCT and IOP were compared with the method of repetitive measure data analysis of variance between the two groups before the operation and 1 week, 4 weeks, 3 months and 6 months after the operation.Results　The CCT of the observation group was thicker than that of the control group before the operation and 1 week after the operation(P<0.05).There were no significant differences in the levels of CCT between the two groups 4 weeks after the operation or longer. The IOP of the observation group was higher than that of the control group before the operation and 1 and 4 weeks after the operation(P<0.05), however there were no significant differences in the levels of IOP between the two groups 3 and 6 months after the operation.Conclusion　The patients who have been attacked by the primary acute angle-closure glaucoma may recover to the normal levels of CCT and IOP after the compound trabeculectomy.]]> 2017/6/28 15:27:54 ZHONG Shan, LI Li 338true ［Abstract］　Objective　To explore the effect of postpartum pelvic floor myoelectricity stimulation on pelvic floor muscle strength and ovarian function in lactating women.Methods　Three hundred lactating women were collected in our hospital from January 2013 to December 2014 and were randomly divided into the observation group and the control group, with 150 cases in each group. The observation group was given postpartum pelvic floor myoelectricity stimulation treatment, and the control group was given Kegel exercise guidance. The pelvic floor muscle strength and the ovarian function were compared between the two groups before and after the treatment.Results　The improvement of the pelvic floor muscle strength in the observation group was significantly better than that in the control group（P<0.05）. The endometrial thickness was thicker, the level of FSH was lower and the level of E₂ was higher in the observation group after the treatment than those before the treatment（P<0.05）. The improvements of endometrial thickness, FSH and E₂ after the treatment in the observation group were superior obviously to the control group（P<0.05）. There were no significant differences in the changes of endometrial thickness, FSH and E₂ after the treatment compared with those before the treatment in the control group（P>0.05）.Conclusion　Postpartum pelvic floor muscle stimulation can significantly improve the strength of pelvic floor muscles and promote the recovery of ovarian function.]]> 2017/6/28 15:27:54 SU Xiao-rong 337true ［Abstract］　Objective　To investigate the clinical value of transrectal ultrasound in locating obstructive sites and making treatment choices for patients with ejaculatory duct obstruction(EDO) azoospermia.Methods　One hundred and twenty-nine patients with EDO azoospermia were performed high frequency and transrectal ultrasound by which the location of ejaculatory duct obstruction was determined and the treatment options were made for the clinicians.Results　Of the 94 patients with EDO azoospermia, 24 patients with lower EDO were treated with transurethral resection of ejaculatory duct by seminal vesicle according to the ultrasonic results. The living semens were found 3 months after the operation, 10 patients′ wives were pregnant and 8 babies were born. The other 70 patients with higher EDO received the recommended intracytoplasmatic sperm injection in oocytes after the live sperms were found by paracentesis of testis or epididymis among whom 44 patients′ wives were pregnant and 39 babies were born.Conclusion　Transrectal ultrasonography can accurately locate the obstructive sites of the ejaculatory ducts. and effectively improves the success rate of treatment by taking the different treatment options according to the different obstructive sites.]]> 2017/6/28 15:27:54 WAN Li-kai, LI Da-wen, WANG Xiao-ping, et al 336true ［Abstract］　Objective　To detect the expressions of Pdlim7 in different histological types of sarcoma and to explore their clinical significances.Methods　Immunohistochemical SP method was used to detect the expressions of Pdlim7 in 214 cases of different histological types of sarcoma and the expressions of Pdlim7 in various sarcomas.Results　There were significant differences in the Pdlim7 expressions among different histological types of sarcomas.Conclusion　The expressions of Pdlim7 are different in different histological types of sarcomas, which may has a certain value in clinical and pathological diagnosis of sarcomas.]]> 2017/6/28 15:27:54 JIANG Shi-jie, BIAN Li-hui, LAN Teng, et al 335true ［Abstract］　Objective　To investigate the nasal vestibule marsupialization and labiogingival groove nasal vestibular cyst resection in the treatment of nasal vestibular cyst.Methods　84 patients admitted to our hospital from February 2012 to January 2015 for the surgical treatment of nasal vestibular cyst were collected as the research subjects, the patients were divided into group A(n=51, treated with the nasal vestibule marsupialization) and group B(n=33, treated with the labiogingival groove of nasal vestibular cyst resection) according to the different operation modes. The operation time, intraoperative bleeding, postoperative pain, hospitalization time and infection, the postoperative facial swelling and the recurrence rate were compared between the two groups 6 months after the operation.Results　The operation time, the intraoperative blood loss, the pain scores 24 hours after the operation and the hospitalization time in group A were shorter or less than those in group B. The facial swelling and postoperative recurrence rates of group A were lower than those of group B.Conclusion　The nasal vestibule marsupialization can reduce more trauma, complications and recurrence rate, promote more postoperative healing than labiogingival groove of nasal vestibular cyst resection in the treatment of nasal vestibular cyst.]]> 2017/6/28 15:27:54 CHEN Yong-zhen, LIANG Tian-xi, XUE Bo-guang 334true ［Abstract］　Objective　To investigate the correlation between serum IL-22 and coronary artery calcification and the predictive value of IL-22 in vascular calcification.Methods　202 patients were divided into low calcification group(LCAC, total scores≤300, with non-coronary heart disease, n=146), and calcification group(HCAC, high total scores>300, with coronary heart disease, n=56). The levels of serum IL-22 were determined by ELISA.Results　The levels of IL-22 were (28.34±12.63)pg/ml in the LCAC group and (38.59±12.90)pg/ml in the HCAC group(t=-6.12, P<0.001). The statistic results showed that there was a significant correlation between the level of serum IL-22 and the coronary calcification scores(HCAC: r=0.42, P<0.01). The area under ROC curve of IL-22 was 0.871, and the optimal critical value was 27.50 pg/ml, with a sensitivity of 85% and a specificity of 71%.Conclusion　The level of serum IL-22 is significantly increased in the patients with severe coronary artery calcification, which may be used as a predictor of coronary artery calcification.]]> 2017/6/5 0:00:00 YANG Zi-cong, LIU Yu, GAN Jian-ting, et al 333true ［Abstract］　Objective　To examine the expression of matrix metalloporteinases-9(MMP-9) protein in pancreatic head cancer tissues and to evaluate the correlation of its clinical significance with pancreatic head cancer.Methods　From January 2000 to December 2007, the surgical resected cancer tissues and adjacent tissues of 38 patients with pancreatic head cancer were collected and their clinical data were analyzed. The expression of MMP-9 protein in all the tissues was detected by immunohistochemistry, and its relationship with clinicopathological features was analyzed. Chi-square test was performed for comparison of rates between the two groups. The overall survival analysis of 38 patients was performed after surgery.Results　The expression rate of MMP-9 protein was 71.1% in the pancreatic head cancer tissues（27/38), and no expression of MMP-9 was found in the adjacent normal pancreatic tissues. The expression of MMP-9 was significantly correlated with tumor size, TNM stages and distant metastasis(P<0.05). However, the expression of MMP-9 was not correlated with gender, age, differentiation and lymph node metastasis(P>0.05). Kaplan- Meier survival analysis showed that the survival time of the patients with pancreaic head cancer in whom MMP-9 was positively expressed was shorter than that of the patients in whom MMP-9 was negatively expressed（P<0.05). Cox regression showed that differentiation, TNM stages and MMP-9 overexpression were the independent risk factors that were related to the prognosis of pancreatic head cancer（P<0.05）.Conclusion　MMP-9 is highly expressed in pancreatic head cancer, and may play an important role in the pathogenesis and prognosis of pancreatic head cancer.]]> 2017/6/5 17:27:11 LEI Ting, JIA Fu-xin 332true ［Abstract］　Objective　To investigate the method and outcome of radiofrequency ablation(RFA) combined with percutaneous ethanol injection(PEI) for primary liver cancer（PLC） in special sits.Methods　The data of 49 tumors in 41 patients with primary liver cancer in special sits treated with RFA combined with PEI were retrospectively analyzed.Results　In the 49 tumors, 43 tumors were treated with percutaneous ultrasound-guided PEI+RFA, other 3 tumors were treated with laparoscopic ultrasound-guided PEI+RFA, and the rest 3 tumors were treated with laparotomy ultrasound-guided PEI+RFA. The outcomes of the treatments were evaluated by contrast-enhanced ultrasound or enhanced CT scanning one month after the ablation. The rate of the tumors ablated completely of percutaneous ultrasound-guided PEI+RFA was 93.02%(40/43). The rate of the other two methods was 100%(6/6). All the patients had no severe complications such as abdominal bleeding and adjacent organ injury, except some mild complications such as mild abdominal pain and fever. All the patients discharged from the hospital after the treatment of ablation.Conclusion　Ultrasound-guided PEI+RFA is an effective, safe and feasible treatment method for the patients with liver cancer in special sits and laparotomy and laparoscopy can improve its therapeutic effect.]]> 2017/6/5 17:27:11 YAO Si-yang, YANG Fu-mao, CHEN Yuan-yuan, et al 331true ［Abstract］　Objective　To investigate the effect of the quantitative exercise prescription(prescription 4.2) on type 2 diabetics.　Methods　The therapy group(n=16) received the grade 4 diabetes quantitative exercise prescription(prescription 4.2) for 43 min per day, 6 days per week, and the control group(n=24) maintained the original exercise habits. Both of the two groups got guidance from diabetes diet, and adjusted their medications according to the blood glucose levels. The levels of FBG, 2hPBG, HbA1c, TC, TG, HDL-C, LDL-C, SBP, DBP, BMI and drug application were observed before and 12 weeks after the exercise therapy.Results　(1)The levels of FBG, 2hPBG, HbA1c and TG were decreased markedly after the treatment than those before the treatment in the therapy group（P<0.05）.(2)There were significant differences in the levels of FBG before and after the treatment in the control group（P<0.05）.(3)There were significant differences in the levels of FBG, 2hPBG, HbA1c and TC between the therapy group and the control group 12 weeks after treatment（P<0.05）.(4)During the observation period, the medicine dosage adjustment in 68.75%（11/16） patients in the therapy group was -53.39%; and the medicine dosage adjustment in 50%（12/24） patients in the control group was +88.89%.Conclusion　(1)The grade 4 diabetes quantitative exercise prescription(prescription 4.2) can improve the levels of FBG, 2hPBG, HbA1c, TG and TC in the patients with type 2 diabetics.(2)The effect of exercise is equal to that of medications or even better than the medications on type 2 diabetics.]]> 2017/6/5 17:27:11 CAI Jin-wei, CHEN Qing-yun, LI Li, et al 330true ［Abstract］　Objective　To investigate the effect of adding arginine in the individual nutrition support on the patients with nasopharyngeal carcinoma.Methods　86 patients were randomly divided into two groups, with 43 cases in each group. The control group received individualized nutrition support and the test group was given arginine plus the same treatment as the control group. The nutritional indexes, adverse reactions, and the rates of chemoradiotherapy discontinuation and completion were compared between the two groups before and after the chemoradiotherapy.Results　After the treatment, there was no significant difference in the decline of serum prealbumin between the two groups, and the declines in hemoglobin, lymphocyte count and bodyweight were gentler, and the incidences of oral mucositis and chemoradiotherapy discontinuation were lower and the completion rate was elevated in the test group than those in the control group(P<0.05).Conclusion　Adding arginine can effectively improve the nutritional status individually and reduce the adverse reactions and chemoradiotherapy discontinuation rates for the patients with nasopharyngeal carcinoma.]]> 2017/6/5 0:00:00 PAN Yu, WU Lunqing, LIU Cuirong, et al 329true ［Abstract］　Objective　To study the influence of neoadjuvant chemotherapy(NACT) on the expression of CK5/6 and p63 in cervical squamous cell carcinoma.Methods　Three cycles Cisplminbased NACT were given to 48 patients with cervical cancer stage ⅠB-ⅡB and the patients received radical hysterectomy three weeks after the treatment. The clinical and histopathological response were observed, and the expressions of CK5/6 and p63 in the samples of cancer tissues were detected before and after NACT using immunohistochemical staining method.Results　After NACT, the total clinical response rate(PR) was 56%（27/48）, and the histopathological response rate was 50%（24/48）. The CK5/6 and p63 were positively expressed in a diffuse and strong manner in all of the 48 samples(100%) before NACT, and were diffusely expressed in all of the 46 samples(100%) with residual cancer after NACT（P>0.05）. The expression intensity in the patients after NACT was weaker than that before NACT.Conclusion　There is no significant influence of NACT on the expressions of CK5/6 and p63 in cervical squamous cell carcinoma. The biological markers of CK5/6 and p63 are useful on the diagnosis of cervical squamous cell carcinoma after accepting NACT.]]> 2017/6/5 17:27:11 JI Yu-Yu, LEI Jia, LI Cheng, et al 328true ［Abstract］　Objective　To evaluate the value of ring type cerumen hook on the removal of nasal foreign bodies in children.Methods　Seventy-two children with nasal foreign bodies during the period from September 2013 to June 2016 were removed with ring type cerumen hook. Their clinical data were retrospectively analyzed.Results　Foreign bodies were removed at one time with ring type cerumen hook under topical anesthesia or without anesthesia in 67 cases, and were removed at two times in 3 cases, under general anesthesia with other instrument in 2 cases. Postoperative nasal mucosal bleeding occurred in 3 cases whose bleeding was stopped by nasal mucosa contraction agent, not by nasal packing.Conclusion　The ring type cerumen hook is safe and efficient for the removal of nasal foreign bodies in children, especially for the foreign bodies that are smooth and round shape in the nasal meatus.]]> 2017/6/5 0:00:00 ZHANG Shao-jie, WANG Rong, LIANG Jian-ping, et al 327true ［Abstract］　Objective　To investigate tuberculosis(TB) and human immunodeficiency virus(HIV) coinfection in Guangxi and its influnenced factors.Methods　1 086 HIV/AIDS patients from a certain hospital for infectious diseases of Nanning, Guangxi were screened for TB/HIV coinfection. Logistic regression model was performed to analyze the factors that influenced TB and HIV coinfection.Results　48.53% of the 1 086 HIV/AIDS patients were coinfected with TB and HIV in which simple TB accounted for 46.49%, TB complicated with extrapulmonary TB accounted for 37.38%, and simple extrapulmonary TB accounted for 16.13%. Multivariate logistic regression analysis showed that there were four major risk factors of getting TB topped the list-male patients(OR=1.472, 95%CI=1.056～2.054), more than fifty years old(OR=1.477, 95%CI=1.111～1.964), lower CD4⁺ T lymphocytes cell count(OR=1.347, 95%CI=1.022～1.776) and higher platelet count(OR=2.079, 95%CI=1.360～3.179).Conclusion　The infection rate of TB is high in the HIV patients of Guangxi. Sex, age, CD4⁺ T lymphocytes cell count and platelet count are the risk factors for HIV/AIDS patients. Different preventing measures should be given to the patients according to their different clinical features.]]> 2017/6/9 8:58:44 MENG Si-run, WU Feng-yao, CHEN Xiao-yu, et al. 326true ［Abstract］　Objective　To evaluate the clinical value of fractional exhaled nitric oxide(FeNO) in wheezy infants and toddlers with asthma predictive index positive(API-positive) or asthma predictive index negative（API-negative） by continuously monitoring the concentrations of FeNO.Methods　A total of 105 wheezy infants and toddlers were enrolled as the experimental subjects and were divided into API-positive group and API-negative group.Twenty healthy children were collected as the control group. The levels of FeNO were determined using the off-line tidal breathing technique in the acute stage, the first month and the third month after the treatment and the levels of FeNO were compared among the three groups.Results　The FeNO levels of the API-positive group were significantly higher than those of the API-negative group and those of the control group in the acute stage or on the first month and the third month after the treatment(P<0.05). In the API-negative group, the FeNO levels of wheezy cases on the first month after the treatment were still significantly higher than those of the controls(P<0.05), but the FeNO levels decreased to the normal level on the third month. In the API-positive group and the API-negative group, the FeNO levels of wheezy cases were significantly lower than those of the controls both on the first month and on the third month after the treatment(P<0.05).Conclusion　Continuously monitoring concentration of FeNO may be used to reflect the airway inflammation in the infants and toddlers with recurrent wheeze and FeNO may be contributed to the guideline of anti-inflammation therapy.]]> 2017/6/9 8:58:44 WEN Zhi-hong, YANG Ying-jie, WANG Mei-chun, et al. 325true ［Abstract］　Objective　To investigate the effect of Simotang oral liquid on the recovery of gastrointestinal function after the operation of esophageal cancer.Methods　110 patients with esophageal cancer were randomly divided into the Simotang group and the saline group, with 55 cases in each group.The conventional nutritional support treatment was given to the two groups. Besides, the Simotong group received Simotang oral liquid via nasojejunal tube after the operation and the saline group were given matched saline(placebo) treatment. The recovery of gastrointestinal function was compared between the two groups.Results　The postoperative anal exhaust time and the bowel sound recovery time in the Simotang group were significantly shorter than those in the saline group(P<0.05), however there was no significant difference in the defecation time between the two groups(P>0.05). There were significant differences in the levels of plasma motilin(MTL) and gastrin(GAS) between the two groups(P<0.05). The concentrations of MTL and GAS increased over time after the operation, in both of the two groups, with significant differences among different time points(P<0.05), and grouping factors interacted with time factors(P<0.05).Conclusion　Simotang oral liquid can effectively promote recovery of the gastrointestinal function, and accelerate healing of the body in the patients with esophageal cancer after the operation.]]> 2017/6/9 8:58:44 WANG Yong-yong, YANG Nuo, DAI Lei, et al. 324true ［Abstract］　Objective　To investigate the clinical effect of anterior nterior approach(OCM) in total hip arthroplasty on the treatment of developmental dyspiasia of the hips.Methods　From June 2009 to June 2014y, 22 cases of developmental dyspiasia of the hip with osteoarthritis were treated with the total hip arthroplasty. Anterior nterior approach was performed on 22 cases. All the patients received unilateral total hip replacement, including 5 males and 17 females. The bleeding volume, operation time, postoperative drainage volume, the operation incision lengths and the amount of hemoglobin were recorded before and after the operation. The harris scores of preoperation, 6 months after the operation and the last time of hip joint function after the operation were recorded.Results　The average operation incision length was (7.5±1.0)cm. The bleeding volume was (260±92.8)ml. The operation time was (70.5±15.6)min. The postoperative drainage volume was (195±45)ml. The amount of hemoglobin before the operation was (121±16.8)g/L. The amount of hemoglobin after the operation was （95±12.6）g/L. The harris scores indicated that the hip joint functions were favorable.Conclusion　OCM approach has a fine early-stage effect on the treament of developmental dyspiasia of the hips.]]> 2017/6/9 8:58:44 HUANG Yu, YIN Dong, HUANG Xiao, et al. 323true ［Abstract］　Objective　To observe the effect of 308 nm excimer lamp on the treatment of vitiligo.Methods　240 patches in 113 cases with vitiligo were irradiated with 308 nm excimer lamp twice a week for 12 weeks. Two weeks later, the efficacy was observed.Results　Of the 240 patches, 83 patches were markedly effective(34.58%), 58 patches effective(24.17%) 85 patches improved and 14 patches ineffective. No adverse reactions occurred in all the 113 cases.Conclusion　308 nm excimer lamp is safe and effective for the treatment of vitiligo.]]> 2017/6/9 8:58:44 MEI Ce-fang, WEN Jing, WU Xiu-lian, et al. 322true ［Abstract］　Objective　To study the characteristic and clinical significance of thyroid nuclear dynamic imaging in the patients with Hashimotos thyroiditis(HT) hypothyroidism with high thyroidal technetium-99m pertechnetate(^99mTc) uptake.Methods　Serum free T3, free T4, sTSH and EBF, PI, UI1, UI2, thyroid stactic uptake of ^99mTc were dectected in 60 patients with HT hypothyroidism in whom 30 cases with high ^99mTc uptake were taken as the study group, and the other 30 cases with normal or low ^99mTc uptake as the control group.Results　EBF, PI, UI1 and UI2 were significantly lower in the study group than those in the control group（P<0.05）. There was a negative correlation between EBF, PI, UI1 and thyroidal ^99mTc uptake（r=-0.623, -0.3402, -0.5719, P<0.05). There was a positive correlation between EBF, PI, UI1 and sTSH（r=0.441, 0.503, 0.473, P<0.05）.Conclusion　More blood to the thyroid gland and more uptake ^99mTc in early duration are the characteristics of thyroid nuclear dynamic imaging in the patients with HT hypothyroidism with high ^99mTc uptake. The results indicate that an increased blood flow to the thyroid gland is the mechanism of high thyroidal ^99mTc uptake in the patients with HT hypothyroidism.]]> 2017/6/9 8:58:44 LIAO Ke-hua, LI Ni, LU Gui-nan, et al. 321true ［Abstract］　Objective　To improve the accuracy of diagnosis and treatments of leukemia being hard to define the types of leukemia by using MICM classification.Methods　The data of leukemia patients were retrospectively analyzed, whose diseases were difficult to diagnose through MICM(Morphology, Immunology, Cytogenetics, and Molecular biology) classification in the past five years in the People′s Hospital of Guangxi Zhuang Autonomous Region.Results　Of the four hundred and sixty-three leukemia patients enrolled in the study, ten cases were difficult to be diagnosed in whom two cases were diagnosed with acute promyelocytic leukemia(AML-M₃, APL) by morphology and immunology classification, but the fluorescence in situ hybridization（FISH） of PML/RARα fusion gene was negative, and three cases were unidentified through morphology classification, and two cases were classified as acute promyelocytic leukemia(APL) and one case as acute myeloblastic leukemia with significant maturation or acute promyelocytic leukemia(AML-M₂/APL) by immunology classification, but by morphology classification, they were defined as AML-M₂; one case showed chronic granulocytic leukemia(CML) on morphology classification, but acute leukemia(AL) on immunology classification; one case was diagnosed with acute myeloblastic leukemia with minimal maturation(AML-M₁) by morphology classification, but in immunology classification, it was considered as acute myeloblastic leukemia with minimal maturation or without cytologic maturation(AML-M₁/M₀) with lymphocytic series expressions, and FISH of BCR/ABL fusion gene was positive.Conclusion　Morphology, immunology, cytogenetics, and molecular biology classification of leukemia may lead to different diagnostic results in the same patient. It is hard to define AML-M₂ and AML-M₃, ALL, AML-M₀ and AML-M₁ through morphology and immunology classification. It needs comprehensive analysis to diagnose the types of leukemia.]]> 2017/6/9 8:58:44 LIN Jin-ying, WANG Bang-qin, ZHOU Rui-lian, et al. 320true ［Abstract］　Objective　To evaluate the clinical efficacy, safety and the prognostic factors of the long-term therapy with entecavir in the patients with hepatic cirrhosis caused by hepatitis B virus.Methods　A total of 120 patients were randomly divided into the treatment group(n=60) and the control group(n=60). The treatment group received entecavir, and the control group received lamivudin.Results　Of the 120 patients, 7 cases died within 48 weeks, and all the dead cases were over 50 years old with Child-Pugh C. 3 cases died in the treatment group, and 4 cases died in the control group, 1 case received liver transplantation because of hepatocellular carcinoma(HCC). 57 cases in the treatment group and 55 cases in the control group were followed up for 144 weeks. The improvement rates of ALT, TBil, ALB, Child-Pugh score in the treatment group were significantly higher than those in the control group(P<0.05). The virological response rate of the treatment group was significantly higher than that of the control group(100% vs 85.4%, χ²=4.46, P<0.05). There were no singnificant differences between the two groups in the rates of serological response, adverse reactions and the terminal adverse events（P>0.05）. The rate of drug resistance in the treatment group was significantly higher than that in the control group(14.5% vs 0%, χ²=5.72, P<0.05).Conclusion　Entecavir has a better effect on antivirus than lamivudin in the treatment of hepatic cirrhosis caused by hepatitis B virus.Age and Child-Pugh score are correlative with the terminal adverse events.]]> 2017/6/14 10:41:48 XU Hong, JIN Shui-zhong, CHEN Hai-yan, et al. 319true ［Abstract］　Objective　To evaluate the outcome and the surgical experience of treating fungal sinusitis.Methods　The clinical data of 85 patients with fungal sinusitis diagnosed in our department from January 2013 to December 2015 were retrospectively analyzed.Results　The lesions were located inmaxillary sinus in 63 cases, in ethmoidal cellules in 6 cases, in sphenoid sinus in 6 cases, and in frontal sinus in 2 cases. Of the 65 cases with fungal maxillary sinusitis, 41 patients were treated with endoscopic maxillary ostium surgery and 24 patients were treated with endoscopic maxillary ostium surgery combined with inferior meatus fenestration. All the patients were followed up for 6 to 12 months after the surgery. The curative rate was 92.7%(38/41) in the patients who were treated with endoscopic maxillary ostium surgery,among whom three cases had nasal adhesion, two had epiphora, and two had facial numbness; The curative rate was 100.0% in the patients who were treated with endoscopic maxillary ostium surgery combined with inferior meatus fenestration, among whom none of the compliations occurred except that two cases had nasal adhesion.Conclusion　Endoscopic maxillary ostium surgery combined with inferior meatus fenestration is a minimally invasive, safe and effective approach for the surgical treatment of fungal maxillary sinusitis.]]> 2017/6/14 10:41:48 XU Liang, LIU Min, TANG Feng-zhu, et al. 318true ［Abstract］　Objective　To study effects of vascular endothelial growth factor(VEGF) on the proliferations of osteoblast in callus in the treatment of fracture nonunion by extracorpoporeal shock wave combined with veronica peregrina.Methods　One hundred New Zealand white rabbits were selected to make a modle of bone nonunion of radius. They were randomly divided into four groups. Group A was treated by extracorporeal shock wave combine with veronica peregrina. Group B was treated by extracorporeal shock wave. Group C was treated with veronica peregrina and group D was taken as the control group. The samples in each group were collected at the end of 1, 2, 4, 8 and 12 weeks respectively after the therapies, and hematoxylin-eosin stain was used to assess the osteoblast counts. VEGF expression was assessed by immunohistochemistry.Results　Compared with those in the other three groups, the proliferation of osteoblast was significantly increased and the expression of VEGF was up-regulated in group A（P<0.01）. The osteoblast counts and the expression of VEGF shared a similar changing trend. The osteoblast counts in each group started to increase obviously at 2 weeks and reached a peak at 8 weeks, and then went down at 12 weeks and persisted on a high level.Conclusion　Extracorporeal shock wave combine with veronica peregrina is safe and effective to treat fracture nonunion. The mechanism involves up-regulating the expression of VEGF and inducing the proliferation of osteoblast.]]> 2017/6/14 10:41:48 ZHAO Zi-xing, LI Hong-yu, XI Li-cheng, et al. 317true ［Abstract］　Objective　To explore and compare the clinical effects between the traditional open reduction and internal fixation in combination with three dimensional(3D) printing digital technique on the treatment of acetabular posterior wall fracture.Methods　The clinical data of 63 cases with acetabular posterior wall fractures in our hospital from May 2014 to February 2016 were respectively analyzed. All the patients were divided into two groups according to the different surgical approaches. The traditional surgery group(n=31) receivd traditional open reduction and the 3D printing openation group(n=32) receivd internal fixation and 3D printing digital techniqne in the treatment of acetabular wall fracture. The operation time, intraoperative bleeding volume, postoperative drainage volume, postoperative hip pain scores and hip function scores were compared between the two groups.Results　All the patients were operated successfully, with no death and complications during hospitalization and follow-up period. All the patients were followed up for 6 to 12 months, with an average of 9.7 months. The operation time, intraoperative bleeding volume and postoperative drainage volume in the 3D printing operation group were significantly shorter or less than those in the traditional surgery group(P<0.05). Two weeks and 3 months after the surgery, the hip VAS and Harris scores in the three dimensional printing operation group were better than those in the traditional surgery group(P<0.05). Six months after the surgery, there were no significant differences in the hip VAS and Harris scores between the two groups(P>0.05).Conclusion　Internal fixation in combination with three dimensional printing digital technique with acetabular posterior wall fractures helps develop individualized treatment regimens for the patients, shortens the operation time, reduces the soft tissue dissection and decreases the volume of blood loss.]]> 2017/6/14 10:41:48 LU Jian-jun, TAN Hai-tao, YANG Ke-qin, et al. 316true ［Abstract］　Objective　To investigate the risk factors of cognitive dysfunction in Parkinson′s disease.Methods　The risk factors of cognitive dysfunction in 165 patients with Parkinson′s disease were assessed by Unified Parkinson′s disease rating scale(UPDRS), Montreal cognitive assessment scale(MoCA), Hamilton depression scale(HAMD) and H-Y rating scale.Results　The survey results of MoCA showed that the incidence of cognitive dysfunction in Parkinson′s disease was 51.51%. Course of the disease, HAMD score, H-Y grading, PUDRS Ⅱ score and PUDRS Ⅲ score in the noncognitive dysfunction group were significantly shorter or lower than those in the cognitive dysfunction group. Multiple logistic regression analysis demonstrated that the education years（OR=0.096）, course of the disease（OR=2.626）, HAMD score（OR=4.038）, H-Y grading（OR=9.157） and PUDRS Ⅱ score（OR=2.223）were the independent risk factors(P<0.05).Conclusion　The education years, course of the disease, HAMD score, H-Y grading,and PUDRS Ⅱ score are the related risk factors of cognitive dysfunction in Parkinson′s disease. More attention and early intervention should be focused on the patients to reduce or delay the occurrence and progress cognitive dysfunction.]]> 2017/6/14 10:41:48 WEI Chun-ying, HAN Min, MO Ying-min, et al. 315true ［Abstract］　Objective　To study the effect of cerebrolysin on the Na⁺-K⁺-ATPase of neurons in ischemic penumbra in rats with acute cerebral ischemia-reperfusion injury.Methods　Two hundred and seventy male Wistar rats were randomly divided into the sham operation group, the control group and the treatment group. All the three groups were further divided into five subgroups according to the time of reperfusion(6 h, 24 h, 48 h, 72 h and 7 d), with eighteen rats in each subgroup. The rat model of ischemic penumbra（2 h） was established by using Longa′s suture insertion. Only the treatment group received medical interventions. The grades of neurological symptoms were observed at the time points of 6 h, 24 h, 48 h, 72 h and 7 d after the reperfusion and then the activities of Na⁺-K⁺-ATPase, the changes of brain water content and the sizes of infarction were detected in the rats′ brain tissues after execution.Results　The activities of Na⁺-K⁺-ATPase began to down-regulate in the control group 6 h after the reperfusion then reached the minimum level at 48 h, and rose again a bit at 72 h and achieved a stable level at 7 d. There were significant differences between the treatment group and the control group in the activities of Na⁺-K⁺-ATPase at the time points of 24 h, 48 h and 72 h after ischemia-reperfusion(P<0.05).Conclusion　Cerebrolysin has a protective effect on neurons in ischemic penumbra of rats with acute cerebral ischemia-reperfusion injury,whose mechanism may be related to improving the activities of Na⁺-K⁺-ATPase.]]> 2017/6/14 10:41:48 SU Liu-fang, XIAO Ji-dong, TANG Shi-ting, et al. 314true ［Abstract］　Objective　To analyze the causes of drug dispensing errors, and to reduce the incidence of dispensing errors and improve the efficiency and quality of drug dispensing in the central pharmacy.Methods　Under the guidance of Plan-Do-Check-Action(PDCA) cycle, the main sources of drugs dispensing errors were analyzed to find the corresponding solutions. The incidence of dispensing errors was compared before and after taking PDCA cycle and the effect of PDCA cycle on improving the quality of drugs dispensing in the central pharmacy was evaluated.Results　The number of drug dispensing errors reduced from 232 cases to 170 cases after the intervention of PDCA cycle, with a significant difference( χ²=28.15, P<0.05).Conclusion　PDCA cycle is effective in improving the drug dispensing work.]]> 2017/6/14 10:41:48 HE Ying, WU Hong-wen, ZHONG Quan-qiang 313true ［Abstract］　Objective　To determine the expression of Aquaporin(AQP) 3 in the dermal skin of a bleomycin(BLM)-induced mouse model of scleroderma, and to explore the possible mechanisms of AQP3 in scleroderma.Methods　Sixty BALB/c mice were divided into PBS group and BLM group, with 30 cases in each group. PBS or BLM was injected subcutaneously in the backs of the mice once per day for 28 days. Ten mice in each group were sacrificed 7, 14, and 28 days after the injection. Hydroxyproline and hydrogen peroxide(H₂O₂) in the skin and AQP3 mRNA and AQP3 protein in the dermal skin were compared between the two groups.Results　28 days after the injection, the levels of hydroxyproline in the skin of the BLM group and the PBS group were (3028.900±485.021) and (1484.500±247.071)(μg/g tissue), respectively, and the H₂O₂ levels were (30.919±8.278) and (6.357±2.337)(μmol/g prot), respectively, and the AQP3 mRNA levels in the dermal skin were (2.090±0.382) and (1.005±0.122), respectively, and the relative gray values of AQP3 protein in the dermal skin detected by Western blots were (2.285±0.552) and 1, respectively. There were significant differences in the levels of hydroxyproline, H₂O₂, AQP3 mRNA and AQP3 protein between the two groups(P<0.05).Conclusion　Higher expression of AQP3 in BLM-treated mice than in PBS-treated mice may be related to its function of transporting H₂O₂.]]> 2017/6/22 9:55:21 LUO Jing-ying, ZHAO Jing-jun 312true ［Abstract］　Objective　To evaluate the effect and clinical value of microendoscopy discectomy(MED) on the treatment of traumatic lumbar intervertebral disc herniation.Methods　The clinical data of 67 patients with traumatic lumbar disc herniation who were performed MED from January 2010 to September 2014 were collected and retrospectively analysed, including 39 males and 28 females. The operation time, blood loss, total volume of drainage after the first postoperative day and the postoperative length of stay were surveyed in all cases. VAS score and modified Macnab scoring criteria were used to evaluate the functional pain relief and restoration of spine.Results　The mean operation time was （53.88±12.33）min. The mean blood loss was （37.53±12.69）ml. The total volume of drainage after the first postoperative day was （16.49±8.96）ml. The average postoperative length of stay was （5.97±1.54）d. Compared with those before the operation, Visual analogue scale(VAS) decreased significantly 1, 3, 6 and 12 months after the operation(P<0.05). According to the modified Macnab scoring criteria, the clinical outcome was excellent in 52 cases（77.61%）, good in 10 cases（14.92%）, acceptable in 4 cases（5.97%）, and 1 case（1.49%） got a worse outcome. The successful rate was 92.54%.Conclusion　MED has a unique advantage in the treatment of traumatic lumbar intervertebral disc herniation.]]> 2017/6/22 9:55:21 MU Xiao-ping, WEI Jian-xun, LAN Min-dong, et al. 311true ［Abstract］　Objective　To investigate the advantages and disadvantages in laparoscopic inguinal hernia repair using home-made super shear system.Methods　The clinical data of two hundred and seventy-one patients with primary inguinal hernia undergoing laparoscopic inguinal hernia repair including totally extraperitoneal, transabdominal preperitoneal, intraperitoneal onlay mesh using home-made super shear system, harmonic scalpel, electrical hook, electrical shear, and electrical clip, were analyzed retrospectively between July 2012 and July 2014. The operative time, the volume of blood, hospital stay after the operation and the complications were compared among the three groups. The patients were divided into three groups, in which group 1(n=92) was subject to super shear system(TEP53, TAPP27, IPOM12); group 2(n=90) was subject to harmonic scalpel(TEP50, TAPP30, IPOM20) and group 3(n=89) was subject to electrical hook, electrical shear, and electrical clip(TEP52, TAPP26, IPOM11).Results　The operative time in the group treated with super shear system was shorter than that in the other two groups(P<0.05 ). There were no significant differences in the volume of blood, hospital stay and the complications after the operation among the three groups(P>0.05).Conclusion　It is safe and effective to perform laparoscopic repair of inguinal hernia using super shear system.]]> 2017/6/22 9:55:21 LI Lin-de, FENG Ze-rong, WU Hong-gen, et al. 310true ［Abstract］　Objective　To study the clinicopathological features and immunophenotypes of follicular dendritic cell sarcoma(FDCS）, and to investigate the relation between Epstein-Barr virus(EBV) and FDCS.Methods　We reviewed the clinicopathological data of 6 patients with FDCS, using microscope observation, immunohistochemical staining and in situ hybridization for EBV encoded nuclear RNA(EBER).Results　Five males and one female were included in this study, with an average age of 34. The tumors were located originally in the lymph nodes in 3 cases and in the spleen, retroperitoneal or pelvic cavity in 1 case, respectively. The inflammatory pseudotumor-like type was found histologically in 1 case and the common type in 5 cases. Immunohistochemically, FDC markers expressed in different levels, and the positive rate of Ki-67 was 2%～30% in the tumor cells. The EBER was positive in the inflammatory pseudotumor-like type FDCS, whereas it was negative in the normal type. All the patients were followed up for 10 to 132 months after tumor resection. Two cases relapsed 10 months and 16 months respectively after the operation. The recurrent sarcoma was found in another lymph node in one case 112 months after the surgery. Nevertheless, this patients survived with the tumor after the sequential successful reoperation and the CHOP-based chemotherapy.Conclusion　FDCS varies largely in histomorphology and immunopheotype, and is obviously different in prognosis.]]> 2017/6/22 9:55:21 HUANG Yong-ta, MO Xiang-lan 309true ［Abstract］　Objective　To investigate the expressions of P16, P53 and Beclin-1 in cervical squamous cell carcinoma(CSCC) and normal human cervical tissues and their ralationships with clinicopathologica parameters.Methods　The expressions of P16, P53 and Beclin-1 protein were detected by immunohistochemical(SP method) in 40 cases of normal human cervical tissues and 43 of CSCC.Results　The expression rates of P16 and P53 in the CSCC were higher than those in the normal cervical tissues(100.0% vs 5.0% and 86.0% vs 5.0%)(P<0.05), and the expression intensity was positively correlated with the level of cervical squamous cell carcinoma. The expression of Beclin-1 in the CSCC(55.8%) was lower than that in the normal cervical tissues(85.0%)(P<0.01), and the expression intensity was negatively correlated with cervical squamous cell carcinoma.Conclusion　The expression levels of P16, P53 and Beclin-1 protein are closely related to the development of CSCC. The expressions of P16 and P53 protein are increased with the progression of cervical epithelial malignant, and the expression of Beclin-1 is down-regulated in malignant cervical lesions.]]> 2017/6/22 9:55:21 ZHANG Xin-ning, WU Hai-yan, LIU Yong-zhu 308true ［Abstract］　Objective　To study the application of craniocerebral injury index（the ratio of GCS/CT score, CCII） in the patients with severe craniocerebral injury(SCCI), and the effect of CCII on the assessment of prognosis.Methods　The clinical data of the patients with SCCI were retrospectively analyzed. The GCS scores and Rotterdam CT scores of CT images, the relationship between the CCII scores and the prognosis, and the relationship between CCII and the severity of the illness were analyzed.Results　The CCII scores of the good prognosis group were significantly higher than the those of the poor prognosis group, and the CCII scores of the group with GCS in the range of 6～8 were significantly higher than those of the group with GCS in the range of 3～5(P<0.05). The CCII scores were positively correlated with the GOS(Glasgow Outcome Rating Scale, GOS) scores(Spearman′s correlation coefficient r_p=0.932,P=0.000). From good to poor prognosis, the CCII scores between the patients with different prognoses trended to reduce gradually（P<0.05).Conclusion　CCII scores contribute to assess the patients′ condition, diagnosis, treatment and the prognosis.]]> 2017/6/22 9:55:21 ZHANG Yi-bin, WANG Jian-qun, CHEN Liang-xin, et al. 307true ［Abstract］　Objective　To compare the clinical results between titanium miniplate in cervical expansire open-door laminoplasty and conventional cervical expansire open-door laminoplasty in the treatment of the developmental stenosis of cervical spinal canal.Methods　The clinical data of 49 patients with developmental stenosis of cervical spinal canal(DSCSC) were retrospectly analyzed.They were divided into two groups according to the different treatment methods.The observation group(n=25) was treated with titanium miniplate, and the control group(n=24) was treated with conventional discectomy. They were evaluated by the method of JOA, the angle of the opened laminae measured by CT or MRI scan at the final follow-up, and the incidence of axial symptoms, the cross angle between posterior vertebral body margins of C₂ and C₇ on cervical radiographs, the operation time and the blood loss were recorded.Results　The period of follow up ranged from 6 to 36 months with an average of (10±3.1)months in the two groups.The improvement rate of JOA scores was (62.5±16.0)% in the observat group and (59.2±9.8)% in the control group six months after the operation, which showed no significant differences between the two groups(P>0.05). The angle of the opened laminae was (43.6±3.5)° in the observation group and (40.5±4.1) in the control group, which showed significant difference between the two groups(P<0.05). The posterior spinal cord shifting was (2.97±1.8)mm in the observation group and (2.96±1.7)mm in the control group,which showed no significant difference between the two groups(P>0.05). There were no significant diferences in cervical curvature angle between the observation group［(18.6±4.8)° vs (18.3±4.8)°］ and the control group［(17.0 ±5.6)° vs (19.9±6.2)°］ before and after the operation. The incidence of axial symptoms in the observation group(12.0%) was significantly lower than that in the control group(41.7%) three months after the operation(P<0.05). No case presented with C₅ nerve root palsy.Conclusion　Both of the two surgical protocols are effective in preventing reclose of opened laminae, however the using of titanium miniplate in cervical expansire open-door laminoplasty is superior to reducing the reclosing and recurrence of axial symptoms.]]> 2017/6/22 9:55:21 OU Yu-fu, LI Xin, WEI Min-ke, et al. 306true ［Abstract］　Objective　To compare the clinical effects between small-dose low-molecular-weight heparin calcium(LMWH) and rivaroxaban combined with conventional methods on the prevention of deep venous thrombosis in elderly patients with hip fractures(including femoral neck fracture and femoral intertrochanteric fracture).Methods　One hundred and six patients(aged ≥75 years) with hip fractures from January 2014 to February 2015, were randomly divided into the small-dose LMWH group(n=56) and the rivaroxaban group(n=50). The efficiency and complication rate of prevention of lower extremity deep venous thrombosis were compared between the two groups.Results　There was no significant difference in the effective rate between the small dose LMWH group(89.3%) and the rivaroxaban group(90.0%)(P>0.05). The bleeding complication rate of the small-dose LMWH group(0.0%) was significantly lower than that of the rivaroxaban group(10.0%)(P<0.05). There was no significant difference in the incidence of prolonged prothrombin time between the small-dose LMWH group(1.8%) and the rivaroxaban group(6.0%)（P>0.05）.Conclusion　Both of small-dose low-molecular-weight heparin calcium and rivaroxaban combined with conventional methods are effective for preventing hip fractures in the elderly patients with lower extremity deep venous thrombosis. The bleeding complication rate in the small-dose LMWH group is lower than that in the rivaroxaban group.]]> 2017/6/22 9:55:21 LI De-jian, CHEN Wu, LIU Chang-sheng, et al. 305true ［Abstract］　Objective　To observe the clinical effect and safety of immunoadsorbents on systemic lupus erythematosus（SLE).Methods　Sixteen patients including fifteen cases with SLE and one case with anti-glomerula basement membrane(GBM) disease were treated with HA280 or DNA230 immunoadsorbent. The autoimmune antibodies and other biochemical indexes of the patients were detected and analyzed before and after the treatment.Results　The titres of antinuclear antibody（ANA） and anti-double sranded antibody（ds-DNA） decreased in 9 cases with SLE after the treatment of HA280(P<0.05), and the titres of ds-DNA declined significantly in 6 cases with SLE after the treatment of DNA230(P<0.05). Anti-GBM antibody turned negative in 1 case with anti-glomerula basement membrane(GBM) disease. There were no significant differences in the other biochemical indexes in all the patients before and after the treatment.Conclusion　Immunoasorbents HA280 and DNA230 are safe and effective to remove the autoimmune antibodies from the patients with autoimmune diseases.]]> 2017/6/30 9:13:09 XIONG Li-jia, YE Kun, LIU Yuan-yuan, et al. 304true ［Abstract］　Objective　To observe the expression levels of Toll-like receptors 4(TLR4) and cyclooxygenase-2(Cox-2) in atherosclerotic rabbits and to explore the effects of atorvastatin on the expressions of TLR4 and COX-2.Methods　A total of 36 New Zealand white rabbits (1.8～2.4 kg) were randomly divided into the normal group(n=12), the model group(n=12) and the treatment group(n=12). Except for the normal group, the other groups were fed with high-fat diet for 8 weeks to establish the atherosclerotic model. After the model was successfully established, the treatment group was fed with atorvastatin 2 mg/(kg·d) for 8 weeks. The levels of peripheral blood lipid were monitored at the time points of 0, 8 and 16 weeks after the treatment. The expressions of TLR4 and COX-2 and the adverse events were compared among the three groups 16 weeks after the treatment.Results　Compared with those in the control group, the levels of serum TC, TG and LDL, and the expressions of TLR4 and COX-2 were significantly elevated in the model group(P<0.01). After the treatment of atorvastatin, the levels of serum TC, TG and LDL were decreased and the HDL level was increased(P<0.05), and the expressions of TLR4 and COX-2 were reduced significiantly in the treatment group(P<0.01). The grading of atherosclerosis under light microscopy was the most severe in the model group, followed by the treatment group, with significant differences between the two groups and the control group(P<0.01).Conclusion　The co-expression of TLR4 and COX-2 may play an important role in the pathogenesis of atherosclerosis. Atorvastatin can effectively reduce the expression levels of TLR4 and COX-2 of atherosclerosis in the rabbit model.]]> 2017/6/30 9:13:09 LI Mao-qing, XU Bi-hong, PENG Zhi-yong, et al. 303true ［Abstract］　Objective　To investigate the expression of C-Jun activation domain binding protein 1(JAB1) in human gallbladder carcinoma(GA).Methods　The expressions of JAB1 protein were detected in 65 cases with GA and in 15 cases with chronic cholecystitis by immunohistochemical method.Results　The positive expression level of JAB1 in the pateints with GA(60.0%) was significantly higher than that in the pateints with chronic cholecystitis(13.3%)（P<0.05）.Conclusion　Elevated expression of JAB1 is closely correlated with GA, and the detection of JAB1 may be helpful to evaluate the early diagnosis and prognosis of GA.]]> 2017/6/30 9:13:09 GU Guo-jian, GU Feng-hua, LIAN Ling-zhi. 302true ［Abstract］　Objective　To investigate the anti-inflammatory and antioxidant effects of alprostadil assisted endovascular interventional treatment for diabetic foot.Methods　One hundred patients with diabetic feet were selected from our hospital during April 2013 and June 2015, and they were randomly divided into the observation group（n=50） and the control group（n=50）. The control group was treated with conventional treatment, and the observation group was treated with alprostadil adjuvant with endovascular interventional treament. The improvements of the clinical symptoms, serum inflammatory cytokines and vascular function were compared between the two groups before and after the treatment.Results　After the treatment, the high-sensitivity C-reactive protein, tumor necrosis factor and interleukin-6 were decreased in both of the two groups which were significantly lower than those in the control group(P<0.05). The improvement of pain relief, numbness and claudication distance extended and the improvement of sensation in the observation group were better than those in the control group(P<0.05). The intima-media thickness, systolic peak velocity and vascular(DI-DO)/DO were decreased in both of the two groups, with singnificantly lower ones in the observation group than those in the control group(P<0.05).Conclusion　Alprostadil adjuvant with endovascular intervention can significantly improve the inflammatory response and the vascular function in lower limbs of the patients with diabetic feet.]]> 2017/6/30 9:13:09 SU Rong-bin, SU Yong-cai, FENG Xin-wu, et al. 301true ［Abstract］　Objective　To study the effect and the clinical significance of orthokeratology on accommodative parameters in juvenile myopia.Methods　Thirty-one teenager myopia patients(62 eyes) aged 8～16 years in our center were recruited in this study. Among these subjects there were 16 males and 15 females. The average spherical equivalent refraction was (-3.71±0.90)D. Before and after the patients were orthokeratology, their accommodative parameters were measured by phoropter, including positive and negative relative accommodation, accommodative amplitude(AMP), accommodative response(accommodative lag), and gradient AC/A. Paired t-testing was used to analyze the related data.Results　Orthokeratology restored the accommodative parameters in teenager myopia patients. AMP reduced from (+7.94±1.44)D before wearing orthokeratology to (+7.06±1.19)D after wearing orthokeratology(t=7.94, P<0.05); Accommodative lag measured by FCC method reduced from (+0.90±0.21)D before wearing orthokeratology to (+0.15±0.26)D after wearing orthokeratology(t=13.79, P<0.05). Gradient AC/A reduced from (4.05±0.83) before wearing orthokeratology to (3.03±0.63) after wearing orthokeratology(t=8.07, P<0.05).Conclusion　The accommodative parameters tend to be normal after wearing orthokeratology in teenager myopia patients, which makes the patients′ accommodation and convergence more harmonious delays the development of teenager myopia.]]> 2017/6/30 9:13:09 LAN Fang-fang, ZHAO Wu-xiao, GAN Lu 300true ［Abstract］　Objective　To study the influence of semen round cells on sperm motility parameters.Methods　Semen samples from 132 infertile men were evaluated retrospectively. The concentration of semen round cells and sperm motility parameters were analyzed by Computer-aided Sperm Analysis(CASA) according to the WHO laboratory manual for the examination and processing of human semen(Fifth Edition).Results　There were significantly negative correlations between the concentration of semen round cells and PR, VCL, VSL, VAP, BCF, LIN, WOB(r=-0.203, P<0.05; r=-0.203, P<0.05; r=-0.202, P<0.05; r=-0.257, P<0.01; r=-0.212, P<0.05; r=-0.188, P<0.05; r=-0.236, P<0.01). The concentration of semen round cells was positively correlated with IM(r=0.217, P<0.05).Conclusion　Semen round cells affect sperm motility parameters and reduce the quality of semen.]]> 2017/6/30 9:13:09 MAO Xian-bao, HUANG Yue-yue, XUE Lin-tao, et al. 299true ［Abstract］　Objective　To study the difference in detection of Hb Q-Thailand between capillary electrophoresis(CE) and high performance liquid chromatography(HPLC).Methods　Ten samples of suspicious Hb Q-Thailand by CE were confirmed with thalassemia gene analysis and DNA sequencing and their results were compared with those detected by HPLC.Results　Ten samples with Hb Q-Thailand and Hb Q-Thailand A2(Hb QA2) were all detected by CE, but Hb Q-Thailand and Hb QA2 were detected respectively in 10 samples and 5 samples by HPLC. Moreover, Hb A2 was wrongly detected in Hb Q-H disease by HPLC. The values of Hb Q-Thailand, Hb A2 and Hb QA2 detected by CE and HPLC were （34.41±21.10）%,（1.68±0.57）%,（0.77±0.10）% and（31.72±21.46）%,（2.43±0.22）%,（0.24±0.24）% respectively. There were significant differences in the detection of Hb A2 and Hb QA2 between the different methods of CE and HPLC（P<0.01）, but there was no significant difference in the detection of Hb Q-Thailand between the two methods（P>0.05）.Conclusion　CE is better in detection of Hb Q-Thailand than HPLC.]]> 2017/6/30 9:13:09 WEI Jin-hua, LI You-qiong, TAN Chun-yan, et al. 298true ［Abstract］　Objective　To explore the regulating effect of long non-coding RNA-H19(LncRNA-H19) on cell proliferation and apoptosis in different acute myeloid leukemia cell lines.Methods　HL-60, THP-1 and U937 cells were transfected with si-H19 and si-NC, respectively. MTT assay was performed to determine the proliferation, and cell apoptosis was assayed by flow cytometry using Annexin V-FITC/PI.Results　The expression of LncRNA-H19 in AML cells was significantly down-regulated after the treatment of si-H19 for 72 hours. Down-regulation of LncRNA-H19 in AML cell lines HL-60, THP-1 and U937 inhibited cell proliferation, and induced cell apoptosis(P<0.01).Conclusion　LncRNA-H19 can regulate cell proliferation and apoptosis in acute myeloid leukemia cell lines.]]> 2017/12/22 10:21:37 WANG Cai-xia, ZHOU Zhi-heng, CHEN Bao-xin, et al. 297true ［Abstract］　Objective　To assess the efficacy and safety of aromatase inhibitors in the treatment of male infertility.Methods　The Cochrane Library, PubMed, EMbase, Web of Science, CBM, Wanfang database and CNKI were searched for targeted clinical trials. Randomized controlled trials(RCT) and quasi-RCTs were identified and analyzed according to the Cochrane Handbook for Systematic Reviews of Interventions. Two reviewers evaluated the quality of trials included independently according to the Cochrane Collaboration′s tool for assessing the risk of bias in the randomized trials. Valid data were extracted for analysis.Results　Four controlled clinical trials involving 178 patients were included. Meta-analysis was not performed because of heterogeneity and the data were summarized in a narrative format. The results showed that （1）Gregoriou et al reported that compared with letrozole, anastrazole presented more benefits in improving sperm counts. （2）Clark et al reported that testolactone was more effective than placebo in elevating the levels of serum FSH, LH and free T. Gregoriou et al reported that anastrazole presented more benefits compared with letrozole in elevating serum LH. （3）Wei Ma et al reported that partners′ pregnancy rate in the treatment group(letrozole) was significantly higher than that in the control group(compound Xuanju capsule)(9/39, 23.08% vs 4/39, 10.26%, P<0.05). The incidence of side effects was low.Conclusion　Limited evidence is available and it shows that, compared with placebo, testosterone is more effective in improving some hormone concentration in infertile men. Anastrazole is more beneficial than letrozole in elevating the level of serum LH and sperm counts. AIs have low side effects and good tolerability. However, the available evidence can not fully affirm the efficacy of AIs on male infertility. More high quality trials with large-scale samples are needed.]]> 2017/12/22 10:21:37 XUE Yu, LIAO Ming, QIN Ai-ping 296true ［Abstract］　Objective　To study the effect of long-time pneumoperitoneum on atelectasis after general anesthesia by means of observating the changes of the functional residual capacity(FRC) and arterial blood gas in the patients undergoing laparoscopic radical gastrectomy for gastric cancer.Methods　30 patients undergoing laparoscopic radical gastrectomy for gastric cancer were collected and their arterial blood samples were taken 10 min after induction of anesthesia（T₀）, and 30 min（T₁）, 60 min（T₂）, 2 h（T₃）and 3 h（T₄） after pneumoperitoneum. The levels of potential of hydrogen(PH), alveolar oxygen partial pressure(PaO₂) and arterial partial pressure of carbon dioxide(PaCO₂) were recored and the level of FRC was detected by the closed-circuit helium dilution method.Results　Compare with those at T₀, the levels of FRC were significantly decreased at T₁, T₂ and T₃（P<0.01）. There was no significant difference in the level of FRC between T₃ and T₄（P>0.01）. There was no significant difference in the level of PaO₂ between T₀ and T₁（P>0.01）,while the levels of PaO₂ were significantly decreased at T₂ and T₃（P<0.01）.Conclusion　Long-time pneumoperitoneum can aggravate atelectasis after general anesthesia, which results in the decrease of PaO₂.]]> 2017/12/22 10:21:37 LI Ming-chuan, YU Jian-hong, LI Ke-zhong 295true ［Abstract］　Objective　To evaluate the effect of resveratrol preconditioning on nuclear factor of kappa B(NF-kappaB) activity during acute lung injury induced by intestinal ischemia-reperfusion(IIR) in rats.Methods　Twenty-four healthy female SD rats were randomly divided into three groups: sham-operated group(S group, n=8), intestinal ischemia-reperfusion mode group(M group, n=8), resveratrol pretreatment group(R group, n=8). Acute lung injury induced by intestinal ischemia-reperfusion(IIR-ALI) was conducted by clamping the superior mesenteric artery(SMA) in rats for 75 minutes followed by 4 hours′ reperfusion. In the R group, rats were treated with resveratrol(15 mg/kg, intraperitoneally) for 5 days before IIR. In the other groups, rats were treated with the same volume of NS for 5 days before operation. All rats were sacrificed after 4 hours′ reperfusion of SMA. The lung tissues were obtained for calculation of wet/dry weight ratio(W/D) and pathological investigation(Hofbauer′s scoring system). The expression of nucleoprotein NF-kappaB p65 was detected using Western blotting and the bronchoalveolar lavage fluid was collected to detect the total protein content and the levels of TNF-α and IL-1β.Results　Compared with those in the S group, the Hofbauer scores, W/D, NF-kappaB p65 expression and BALF indexes including the total protein content and the levels of TNF-α and IL-1β in the M group and the R group were increased significantly(P<0.05). Compared with those in the M group, the Hofbauer scores, W/D, NF-kappaB p65 expression of lung tissue and BALF indexes in the R group were decreased significantly(P<0.05).Conclusion　Resveratrol preconditioning can attenuate the acute lung injury induced by intestinal ischemia-reperfusion in rats. This effect may be related to inhibiting NF-kappaB activity in the lungs.]]> 2017/12/22 10:21:37 YANG Jian-yu, HUANG Xiao-lei, LI Bao-zhen, et al. 294true ［Abstract］　Objective　To observe the influence of GC tooth mousse on periodontal index in patients with root dentinal hypersensitivity after subgingival scaling, and to investigate the influence of dentine hypersensitivity on the maintenance of oral health and curative effect.Methods　A total of 464 teeth in 116 cases with dentin hypersensitivity were divided into the treatment group(n=240, 60 cases were treated with GC tooth mousse) and the control group(n=224, 56 cases received no desensitization treatment). The changes of calculus index(CI) and debris index(DI) were obsereved 1 week and 1 month after the treatment.Results　The values of CI and DI in the control group were significantly elevated than those in the treatment group 1 week after the treatment. The values of CI and DI in the control group were significantly elevated than those in the treatment group 1 month after the treatment.Conclusion　The patients with root dentinal hypersensitivity are more likely to keep oral hygiene after the treatment of GC tooth mousse.]]> 2017/12/22 10:21:37 WEI Yi, ZHOU Yan, CHEN Yan, et al. 293true ［Abstract］　Objective　To observe the expression of high mobility group box-1 protein(HMGB1) in the renal tissues of the patients with IgA nephropathy and the relationship between urinary HMGB1 and pathology, and to explore the prediction effect of HMGB1 on the severity of renal histological lesions in IgA nephropathy.Methods　300 patients received renal biopsy, among whom 112 cases were diagnosed with IgA nephropathy and 188 cases with non-IgA nephropathy. The clinical data were collected and the levels of HMGB1 were measured. The semi-quantitative score of renal pathology was recorded and the predictive efficiency of HMGB1 was analyzed by ROC curve.Results　(1)The average urinary HMGB1 concentration of the patients with IgA nephropathy was (1.5268±0.7116)mg/μmol Cr. (2) The HMGB1 expressions were found in the renal tubules, interstitial and glomerular cell nuclei, and the expressions varies with different severe lesions and ranges. (3)When the urinary HMGB1 was used to predict the severe mesangial cellularity(optimal cut-off 1.8191 mg/μmol Cr, 95%CI=1.2573～2.2622), the area of receiver operating characteristic curve(AUROC) was 0.738(95%CI=0.646～0.818) with a sensitivity of 70.0% and a specificity of 77.8%. When the urinary HMGB1 was used to predict the severe glomerulosclerosis(optimal cut-off 1.4954 mg/μmol Cr, 95%CI=1.3162～1.7392), the AUROC was 0.842(95%CI=0.760～0.904) with a sensitivity of 95.8% and a specificity of 67.4%. When the urinary HMGB1 was used to predict the severe tubule-interstitial damage(optimal cut-off 1.4645 mg/μmol Cr, 95%CI=1.3495～1.4645), the AUROC was 0.827 (95%CI=0.743～0.892), with a sensitivity of 96.6% and a specificity of 67.9%(P<0.05).Conclusion　The levels of urinary HMGB1 are closely related to the mesangial cellularity, glomerulosclerosis and tubule-interstitial damage in IgA nephropathy, which might be used as one of the potential biomarkers for determining the pathological severity of IgA nephropathy.]]> 2017/11/28 17:12:43 LIN Xin, YIN Lin, LIU Juan, et al. 292true ［Abstract］　Objective　To explore the clinical effect of Shaolin orthopedics-traumatology prescription combined with acupuncturing acupuncture point on postoperative pain for patients with limb fractures.Methods　180 patients with limb fractures were collected in the Traditional Chinese Medicine Hospital of Yangjiang City and were divided into the observation group and the control group according to the different analgesic methods. The clinical effects were compared between the two groups.Results　Before treatment, there were no significant differences in VAS scores between the two groups(P>0.05). After treatment, the VAS scors of the observation group were superior to those of the control group（P<0.05）. The excellent rate of analgesia in the observation group(100%) was significantly higher than that in the control group(86.66%)(χ²=12.857, P=0.000). The incidence of adverse events in the observation group(3.33%) was significantly lower than that in the control group(38.88%)(P<0.05).Conclusion　Shaolin orthopedics-traumatology prescription combined with acupuncture point is effective for the postoperative pain in the patients with limb fractures.]]> 2017/11/28 17:12:43 LI Jun-hao, ZHAO Jian-ming, ZHANG Shuai, et al. 291true ［Abstract］　Objective　To explore the clinical effect of a new approach of spinal external fixation combined with vertebroplasty or vertebral bone graft on thoracolumbar fractures.Methods　The clinical data of 221 patients with thoracolumbar fractures were collected in our hospital from July 2011 to July 2016 and were retrospectively analyzed. External fixation instruments were initially used for fracture reduction and then vertebroplasty(for osteoporotic fracture) or vertebral bone graft(for traumatic fractures) was performed. The injury vertebral heights at the time of preoperation, postoperation and the final follow-up according to the radiographic data, kyphotic Cobb Angle and visual analogue scale(VAS) were recorded.Results　All the 221 patients improved significantly after surgery and at the time of the final follow-up in injured vertebral height, kyphotic Cobb Angle and visual analogue scale(P<0.05). There were no significant differences in the indicators between the postoperative results and the final fellow-up results. Major complications occurred in 24 cases,including cement leakage in 13 cases and vertebral height loss in 11 cases, without complicated nerve impairment, screw loosening and screw fractures.Conclusion　External fixation instruments are safe, effective and minimally invasive in treatment of thoracolumbar fractures.]]> 2017/11/28 17:12:43 WU Wei-qiang, CHEN Yuan-ming, HUANG Zhong-fei, et al. 290true ［Abstract］　Objective　To explore the non-coordination limitation of upper and lower jaws(angle ANB) in adults with skeletal class Ⅱ malocclusion who were treated with camouflage fixed appliance and had good occlusion.Methods　38 adult patients who had skeletal class Ⅱ malocclusion and completion of camouflage fixed treatment were selected. Their hard plaster dental casts and cephalometric films were collected before and after the treatment. The values of measurement items on PAR index, ∠ANB and ∠U1-SN were obtained and the correlations between them were analyzed using statistic software SPSS16.0.Results　(1)The good occlusion was obtained in all the 38 cases after the treatment. (2)Before treatment, ∠ANB had positive or negative linear correlation with the changes of weighted PAR scores and ∠U1-SN after fixed treatment.Conclusion　Most of the adults with skeletal class Ⅱ malocclusion can obtain good occlusion after the treatment of camouflage fixed appliance.However the orthognathic surgery is strongly recommended to the patients when ∠ANB is equal to or more than 9°.]]> 2017/11/28 17:12:43 HUANG Dong-sheng, FU Li-li, HUANG Min-fang 289true ［Abstract］　Objective　To investigate the application of peritoneal dialysis for the patients with end-stage renal disease, and to provide the basis for improving the quality of peritoneal dialysis.Methods　The cross-sectional study of peritoneal dialysis was performed on the patients with end-stage renal disease in 158 hospitals in Guangxi from 2014 to 2016. The number of the patients with peritoneal dialysis, peritoneal dialysis related peritonitis, floating pipe, pipe blockage, blood pressure, hemoglobin, calcium and phosphorus metabolism and parathyroid hormone(PTH) of reaching standard were analyzed and compared among the three years.Results　Peritoneal dialysis technology was carried out only in 30 hospitals in the survey. The number of the patients receiving peritoneal dialysis equal to or more than 25 cases was 11, 11 and 12 in 2014, 2015 and 2016 respectively. The rates of the patents transferred from peritoneal dialysis to hemodialysis in 2014, 2015 and 2016 were 3.6%, 4.8% and 5.4%(P>0.05) respectively, and their mortality rates were 7.5%, 4.5% and 4.0%(P<0.05). Peritonitis that related to peritoneal dialysis was the major complications during peritoneal dialysis. The rates of the patients whose blood pressure was well controlled were 42.5%, 61.0% and 72.1% in 2014, 2015 and 2016 respectively(P<0.05). The rates of hemoglobin reaching the standard were 45.7%, 46.7% and 45.8% in 2014, 2015 and 2016 respectively(P>0.05). the rates of calcium and phosphorus metabolism that were well-controlled were 60.9%, 73.8% and 60.9% in 2014, 2015 and 2016 respectively(P<0.05). The rates of PTH that were well-controlled were 45.3%, 49.5% and 46.9% in 2014, 2015 and 2016 respectively(P>0.05).Conclusion　Peritoneal dialysis technology is carried out in a small number of the hospitals in Guangxi. The mortality rate of the patients with peritoneal dialysis has declined over the past three years, and the quality of peritoneal dialysis such as the blood pressure, calcium and phosphorus metabolism has been improved to some extent.]]> 2017/11/28 17:12:43 LIU Yuan-yuan, PENG Xiao-mei, WU Chao-qing, et al. 288true ［Abstract］　Objective　To investigate the risk factors of surgical site infection(SSI) following spinal internal fixation surgery.Methods　From April 2015 to June 2016, the retrospective survey was conducted for the patients who underwent spinal internal fixation operation in the department of orthopaedics, and the incidence and risk factors of SSI were analyzed.Results　The incidence of SSI was 2.72%(11/405). Univariate analysis showed that age, gender, NNIS, the operation duration, intraoperative blood loss, transfusion and cerebrospinal fluid leakage were related to SSI. The multiple logistic regression analysis showed that age(>60 years)（OR=4.10, 95%CI=1.01～16.75）, the operation duration(>4 h)（OR=5.20, 95%CI=1.29～21.03） and cerebrospinal fluid leakage were the independent risk factors of SSI.Conclusion　Occurrence of the surgical site infection following spinal internal fixation surgery is related to multiple factors, and therefore the corresponding prevention and control contermeasures should be taken to reduce the incidence of SSI.]]> 2017/11/28 17:12:43 TANG Yu-mei, YIN Dong, XIE Pei-yan, et al. 287true ［Abstract］　Objective　To explore the high-risk factors, pathogenesis and clinical manifestions of placental abruption.Methods　The clinical data of 167 cases with placental abruption in Wuhan Children Hospital from January 2007 to December 2012 were retrospectively reviewed.Results　The incidence of placental abruption was 0.56% in 167 cases,among whom 105 cases had the main predisposing factors(62.87%), including premature rupture of fetalmembranes(PROM), hypertensive disorders, complicating pregnancy, traumatic factors, twins and hasten parturition. The main clinical manifestations included vaginal hemorrhage, abdomen pain, fetal heart rate abnormalities and uterine hypertonia. The ultrasonography detection rate was 39.74%. The first-degree placental abruption rate was 54.49%. The second-degree placental abruption rate was 22.75%, and the third-degree placental abruption rate was 22.75%. It needed more time for the patients with the second and the third degree of placental abruption from the onset of the initial clinical sign to the treatment than the patients with the first degree of placental abruption, but there was no significant difference in the time between the two groups(P>0.05). After the delivery, postpartum hemorrhage occurred in 30 cases,uteroplacental apoplexy in 31 cases, subtotal hysterectomy in 4 cases, disseminated intravascular coagulation(DIC) in 3 cases, fetal death in 10 cases. and 71 premature neonates were transferred to the neonatal intenisve care unit(NICU).Conclusion　There are nonspecific clinical manifestations of placental abruption. Some cases have high-risk factors while others are misdiagnosed by ultrasound examination. The time from onset of the initial clinical signs to the treatment affects the prognosis of placental abruption.]]> 2017/10/26 8:15:40 LIU Jiu-ying, WANG Lin, WANG Chuan-xiang, et al. 286true ［Abstract］　Objective　To analyze the features and risk factors of occupational exposure for medical staff and to explore the prevention of occupational exposure in infectious diseases hospital.Methods　The data of the workers with occupational exposure to human immunodeficiency virus(HIV) among the medical staff from January 2012 to December 2014 in our hospital were respectively analyzed.Results　Among the 41 cases with occupational exposure to HIV, 21 cases were nurses(51.2%), 8 cases were nurse practitioners(19.5%), 2 cases were supervisor nurses(4.9%), 31 cases were nursing attendants(75.6%), 5 cases were cleaning workers(12.2%), and 2 cases were attending physicians(4.9%) and physicians(4.9%) respectively. 23 cases exposed to HIV while withdrawing and sealing the indwelling needles(56.1%). 9 cases exposed to HIV while injecting drugs or drawing blood(22.0%). 5 cases exposed to HIV while disposing the medical waste(12.2%). The main causes for occupational exposure were needling(75.6%) and splashing of blood or body fluid into the mucous membrane of eyes(24.4%).Conclusion　Professional safety training should be strengthened. The standard prevention measurements and the regulation complying with operations should be strictly implemented. The complete and convenient reporting system and addressing procedure of professional exposure should be established to reinforce the management and to prevent the risk of HIV occupational exposure.]]> 2017/10/26 8:15:40 LIU Dong-mei 285true ［Abstract］　Objective　To summarize the experience of minimally invasive off-pump transthoracic closure in treatment of ventricular septal defect(VSD), and to evaluate its clinical effect and safety.Methods　From March 2016 to November 2016, 10 patients with VSD(2 males and 8 females, weighed 7 to 48 kg with a mean of 19.35 kg, aged 9 months to 21 years) underwent a closure of VSD through transthoracic using Amplatzer occlude from right ventricular surface. Before the operation, all the cases had been diagnosed with a 3 to 8 mm VSD by transthoracic echocardiography(TTE).Results　5 cases were cured with satisfaction. No arrhythmia, no residual shunt, no valve regurgitation, no atrioventricular block, no occlude displacement, and no embolization occurred on discharge. The other five cases were converted to cardiopulmonary bypass.Conclusion　The minimally invasive off-pump transthoracic closure is safe and feasible for treating VSD, with advantages of small incision and quick recovery.]]> 2017/10/26 8:15:40 ZHONG Yong-long, LI Xiang-wei, HUANG Jing-bin, et al. 284true ［Abstract］　Objective　To investigate the expression of serum hepcidin and its relationship with 25 hydroxy vitamin D［25(OH)D］ in patients with chronic kidney disease(CKD) stage 5.Methods　85 patients with CKD stage 5 were enrolled, among whom 55 cases were treated with maintennance hemodialysis(MHD), and the other 30 cases received non-dialysis treatment. 30 healthy people were taken as the control group. The levels of serum hepcidin, 25(OH)D, hemoglobin(Hb), ferritin(SF), serum iron(SI), transferrin saturation(TSAT) and c-reactive protein(CRP) were detected and compared among the three groups.Results　The levels of hepcidin in the MHD group, non-dialysis group and the control group were （91.8±11.3）μg/L, （58.9±5.27）μg/L and （46.9±5.95）μg/L respectively. The levels of 25(OH)D in the above three groups were （25.9±3.25）μg/L, （29.2±4.21）μg/L and （36.9±3.65）μg/L respectively. The level of hepcidin was significantly higher and the level of 25(OH)D was significantly lower in the MHD group than those in the non-dialysis group and the control group(P< 0.05). Of the 85 patients with CKD stage 5, 28 cases had a 25(OH)D deficiency and 32 cases had a 25(OH)D insuffciency. Compared with those in the normal group, the levels of hepcidin and CRP in the deficiency group were significantly higher, PTH in the insuffciency group was significantly higher, and hemoglobin was significantly lower in both of the two groups(P<0.05). Hepcidin was negatively correlated with Hb, SI and 25(OH)D but positively correlated with SF and CRP. Multiple stepwise regression showed that SF, CRP and Hb were closely related to hepcidin in the patients with CKD stage 5.Conclusion　Hepcidin is elevated in the patients with CKD stage 5. Inflammation, iron storage and anemia affect the expression of hepcidin. Hepcidin is elevated in the patients with 25(OH)D deficiency, but is not associated with 25(OH)D.]]> 2017/10/26 8:15:41 ZHONG Jian, ZHANG Wen-xin, TANG Sheng, et al 283true ［Abstract］　Objective　To investigate the expression of transmembrane protease serine 4(TMPRSS4) protein in non-small-cell lung cancer(NSCLC) and its clinical significances.Methods　The expressions of TMPRSS4 were detected in the tissues of NSCLC and their matched adjacent normal tissues in 45 patients by the immunohistochemical streptavidin peroxidase(SP) technique.Results　The positive expression rate of TMPRSS4 was 60.0% in the NSCLC cancer tissues, and 4.44% in the matched adjacent normal tissues. The positive expression rate of TMPRSS4 in the NSCLC tissues was significantly higher than that in the pericarcinomatous tissues(χ²=29.31, P<0.01). The expression of TMPRSS4 protein was related to the metastasis and clinical stages of NSCLC.Conclusion　The expression of TMPRSS4 protein is related to the metastasis and clinical stages of NSCLC and the overexoression of TMPRSS4 indicates the high risk of metastasis and poor prognosis, which may be a novel research hotspot in NSCLC.]]> 2017/10/26 8:15:41 LI Shou-nan, WU Zheng-qiu, LU Qiang-chang, et al. 282true ［Abstract］　Objective　To investigate the expression of miR-155-5p in nasopharyngeal carcinomas（NPC） and its relationship between metastasis and prognosis in NPC patients. Methods　The biopsy specimens of 60 NPC patients with distant metastasis(metastatic group) or primary cancer focus alone(non-metastatic group) in our hospital were collected, and the expressions of miR-155-5p in the specimens were detected by real time fluorescent quantitative PCR(QRT-PCR). The relationship between miR-155-5p and the clinicopathological parameters and prognosis were analyzed. Results　Compared with that in the non-metastatic group,the level of miR-155-5p in the metastatic group was significantly up-regulated［（4.92±5.52） vs （1.98±2.66）］（t=-2.505,P=0.017）. No significant differences were observed among miR-155-5p and sex,age,location, lymph node metastasis, T grades and clinical stages(P>0.05). If the median level of miR-155-5p was considered as cut-off value, Kaplan - Meier survival curve and the Log-rank test results showed that the overall 5 year survival rate of the NPC patients with low expression of miR-155-5p was significantly higher than that of the NPC patients with high expression of miR-155-5p(χ²=4.18, P=0.041 ). Conclusion　The expression level of miR-155-5p may play a key role in metastasis of NPC. It may be a novel biomarker in predicting prognosis of NPC.]]> 2018/9/30 16:15:18 HUANG Yong-ta, YE Qiu-rong, WENG Jing-jin, et al. 281true ［Abstract］　Objective　To investigate the relationship between cathepsin S(Cat S)/cystatin C(Cys C) and the stenosis degree of carotid artery in cerebral infarction patients. Methods　336 patients with atherosclerosis cerebral infarction were recruited and were divided into mild stenosis, moderate stenosis and severe stenosis groups according to different stenosis intensities. During the same period, 114 healthy examinees were recruited as the health controls. The bloods of all the patients and the control group were collected and the Elisa and latex-enhanced immunoturbidimetric assay methods were used to detect the serum levels of Cat S and Cys C respectively at day 1 and 14 after stroke occurance. Results　The levels of Cat S, Cys C and ratio of Cat S/Cys C had significant differences(P<0.05) between the mild stenosis group, the moderate stenosis group, the severe stenosis group and the control group. With the increase of vascular stenosis, the levels of Cat S and ratios of Cat S/Cys C were positively and Cys C was negatively correlated with the vascular stenosis severity. Spearman correlation analysis showed that the level of Cat S was positively correlated with the unstable plaque(r=0.475, P<0.05). 14 days after stroke, the levels of Cat S and ratios of Cat S/Cys C in all the patients were relatively reduced and the levels of Cat C were higher compared with those 1 day after stroke(P<0.05). Conclusion　In the patients with cerebral infarction, Cat S is positively correlated with the severity of vascular stenosis, and Cys C is negatively correlated with the severity of vascular stenosis. The changes of Cat S/Cys C are more significant in the patients which are the predictors for diagnosis of cerebral infarction and the severity of vascular stenosis.]]> 2018/9/30 16:15:18 HAN Xin-sheng, ZHANG Hong-yang, HAN Miao, et al. 280true ［Abstract］　Objective　To investigate the incidence of anxiety and depression in patients with primary trigeminal neuralgia and to analyze the related factors. Methods　One hundred and sixty-eight patients diagnosed with primary trigemial neuralgia(58 males and 110 females) were included in the study. The demographic data of all the subjects were collected. The severity of pain was assessed with Visual Analogue Scale(VAS), and the anxiety and depression were evaluated with Zung Self-rating Anxiety Scale(SAS) and Zung Self-rating Depression Scale(SDS), respectively. The demographic data, the scores of the above scales and the related factors were analyzed. Results　Of the 168 patients with primary trigeminal neuralgia, 40 cases(23.8%) had anxiety, 47 cases(28.0%) had depression, and 22 cases(13.1%) had both anxiety and depression. The patients with pain scores ≥7 had significantly higher incidence of anxiety and depression than those with pain scores<7. The incidence of anxiety symptoms in the patients receiving one treatment was significantly lower than that in the patients receiving two or more treatments. The depression rate in the patients with pain in the right side was significantly higher than that in the patients with pain in the left side. The incidence of depressive symptoms in the patients with the course of illness more than 5 years was significantly higher than that in the patients with the course of illness less than 5 years(P<0.05). Conclusion　The patients with primary trigeminal neuralgia have higher incidence of anxiety and depression which are affected by many factors.]]> 2018/9/30 0:00:00 FANG Jian-zhong, LIAO Sheng-chao, SHAO Chang-jiang, et al. 279true ［Abstract］　Objective　To explore the effects of locked plating on proximal humeral fracture complicated with the defect of medial cortical bone and to compare the reconstructing medial column support of proximal humeral fracture with and without bone grafts in adult patients. Methods　The clinical data of 46 cases with proximal humeral fracture who had undergone locked plating in the First People′s Hospital of Nanning City between June 2010 and February 2016 and had been followed-up for (19.8±2.48)months averagely(14～41 months) were retrospectively analyzed. The patients included 28 males and 18 females with the age of 61～92 years old(72.1±3.68)years. The fractures were divided into two types according to Neer Classfication: three-part fractures(31 cases) and four-part fractures(15 cases). All of the cases with medial comminuted fracture of proximal humerus were treated by replacement, and complicated with the bone defects at the affected part during replacement. The 46 cases were divided into the bone graft group(n=20) and the non-graft group(n=26). The reconstruction of the medial column support of proximal humeral fracture was performed for the cases in the two groups by medial support screws. The fracture healing time, Constant-Murley scores of shoulder joint, visual analgesia scores(VAS), varus angle of humerus and the incidence rate of complications were compared between two groups at the time of the latest follow-up. Results　The follow-up was carried out for 14～41 months(19.8±2.48) after operation. The Constant-Murley scores of shoulder joint, VAS, varus angle of humerus and the incidence rate of complications were (76.0±7.9)points, (2.7±0.8)points, (0.7±1.2)° and 15.00% in the bone graft group, and those were (75.5±6.5)points, (3.1±1.1)points, (1.2±1.0)° and 19.23% in the non-graft group(P>0.05). The fracture healing time of the bone graft group(14.6±2.4 weeks) was significantly shorter than that of the non-graft group(16.2±2.9 weeks)(P<0.05). Conclusion　In the treatment of proximal humeral fracture without bone graft, locking plate combined with the reconstruction of the medial column support by medial support screws can get better fixation and clinical effects for the medial comminuted fracture of proximal humerus complicated with bone defects or severe defects of medial cortical bone.]]> 2018/9/30 16:15:18 JIANG Guo-hua, LAO Chen-deng, YUAN Guang-zhi 278true ［Abstract］　Objective　To compare the effects of selective laser trabeculoplasty(SLT) and travoprost eye drops on the treatment of residual advanced primary open-angle glaucoma(POAG). Methods　A total of 30 patients(30 eyes) were divided into two groups using a non-randomized controlled trial methed. The patients in the SLT-treated group received SLT treatment, and the patients in the drug-treated group received 0.004% travoprost eye drops treatment. The patients were followed up for 1 day, 1 week, 1 month, 3 months and 6 months after treatment. The changes of intraocular pressure(IOP) were observed before and after treatment. Results　Before treatment, the difference of IOP between the SLT-treated group and the drug-treated group was not statistically significant(P>0.05). From 1 day to 6 months after treatment, the IOPs were significantly lower than those before treatment in the two groups(P<0.05). 3 months after treatment, there was no significant difference in IOP between the SLT-treated group and the drug-treated group(P>0.05), while at the other time points, there were significant differences in IOPs between the two groups(P<0.05). Compared with the baseline IOP before treatment, the IOPs of the two groups were significantly different 1 day, 1 week, 1 month, and 6 months after treatment(P<0.05). The decrease of IOP in the SLT-treated group was larger than that in the drug-treated group on the 1st day after treatment, and the decrease of IOP in the drug-treated group was greater than that in the SLT-treated group at the other time points. The fluctuation of IOP in the SLT-treated group was greater than that in the drug-treated group after treatment. There was no significant difference in the incidence of postoperative complications between the two groups（P>0.05）. Conclusion　SLT can effectively reduce the intraocular pressure of residual advanced POAG, but the effect and stability of IOP reduction are inferior to those of travoprost eye drops.]]> 2018/9/30 16:15:18 ZHANG Shao-yang, LI Li, ZHONG Shan 277true ［Abstract］　Objective　To evaluate the effects of thymosin combined with GP regimen on clinical outcome, safety and immune function in treatment of advanced non-small-cell lung cancer(NSCLC). Methods　One hundred and fourteen patients with advanced NSCLC were included in this study and randomly divided into control group(n=61) and treatment group(n=53). The patients in the control group were treated with chemotherapy of gemcitabine 1 000 mg·m^-2, day 1, 8+cisplatinum 75 mg·m^-2, twenty-one per cycle. The patients in the treatment group received the same treatment as the control group plus thymosin injection 20 mg qod for 2 cycles of the chemotherapy regime. The clinical efficacy, chemotherapy-related toxicities and the changes of T lymphocyte subsets were compared between the two groups after two cycles of the chemotherapy regime. Results　The clinical benefit rate of the treatment group（86.79%） was significantly higher than that of the control group（P<0.05）. The objective response rate(CBR) of the treatment group（37.73%） was slightly higher than that of the control group（32.79%）（P>0.05）. T-lymphocyte cell subsets of CD3⁺, CD4⁺ and CD8⁺ and NK cells in the treatment group were significantly higher than those in the control group（P<0.05）. CD4⁺/CD8⁺ in the treatment group was significantly higher than that in the control group（P<0.05）. Hematologic toxicities and gastrointestinal responses were the main adverse reactions. The incidence rates of Ⅲ+Ⅳ° leukopenia and thrombocytopenia in the treatment group were significantly lower than those in the control group（P<0.05）. There were no significant differences in adverse reactions including gastrointestinal reactions and abnormal hepatic and renal functions between the two groups（P>0.05）. Conclusion　Thymosin combined with GP regimen is effective in treatment of advanced non-small-cell lung cancer. It can elevate the level of T lymphocyte subsets, reduce the chemotherapy-related toxicities and enhance the patients′ tolerance to chemotherapy.]]> 2018/9/30 16:15:18 LAN Bi-quan, ZHANG Hua-jun, YANG Di-hong, et al. 276true ［Abstract］　Objective　To evaluate the efficacy of circulatory thermo-chemotherapy combined with S-1 and endogenic field thermotherapy in treatment for elderly patients with ascites caused by advanced gastric carcinoma and the adverse reactions of the treatment. Methods　42 patients with gastric carcinoma were randomly divided into the observation group(receiving circulatory thermo-chemotherapy and cisplatin combined with S-1 and endogenic field thermotherapy) and the control group(receiving S-1 monotherapy combined with endogenic field thermotherapy). During the monotherapy of oral S-1, endogenic field thermotherapy(every three days as a cycle, 3 cycles totally) was given to the two groups, and the observation group was given additional circulatory thermo-chemotherapy(three weeks as a cycle, 3 cycles totally) with cisplatin. The curative effects and adverse reactions were evaluated after 2 cycles of treatment. Results　The primary tumor efficiency rate was 57.1% in the observation group and 42.9% in the control group(P>0.05). The objective response rate of ascites was 81.0% in the observation group and 47.6% in the control group(P<0.05). The ascites negative conversion rate was 81.0% in the observation group and 38.1% in the control group. There was no significant difference in the incidence of adverse reactions between the two groups(P>0.05). No serious adverse reactions were found in the two groups after therapy and no treatment-related deaths occurred. Conclusion　Circulatory thermo-chemotherapy and cisplatin combined with S-1 and endogenic field thermotherapy is safe and effective for the elderly patients with advanced gastric carcinoma with ascites.]]> 2018/9/30 16:15:18 WEI Yan, LU Xiao-ling, YUAN Hao, et al. 275true ［Abstract］　Objective　To collect and sort out Ge Hong′s academic thoughts, and to make Gzhi′s Lumbago Prescription based on “Zhouhou Beiji Fang”（Handbook of Prescriptions for Emergency） and to study the effect of ancient prescription and treatment on lumbar disc herniation to provide reference for further study. Methods　200 hospitalized patients with lumbar disc herniation were selected as the study objects in our hospital from June 2015 to May 2017, and were randomly divided into two groups, with 100 cases in each group. The control group was treated with diclofenac sodium sustained-release tablets+Mannitol, and the observation group was treated with Gzhi′s Lumbago Prescription. The syndrome scores were evaluated by the criteria for the evaluation of curative effect for lumbar intervertebral disc herniation(JOA), and the Visual Acuity Score(VAS) and Swestry Lower Aack and Lower Limb Dysfunction Score Scale were used to evaluate the degree of lower back pain and back and lower extremity dysfunction before and 2 weeks after treatment. The clinical effects and adverse reactions were compared between the two groups. Results　Compared with those before treatment, the VAS scores and Swestry scores were significantly improved in both of the two groups(P<0.05). The scores of the observation group were significantly higher than those of the control group after treatment(P<0.05). The clinical effect of the observation group was better than that of the control group after treatment（Z=-5.828, P=0.000）. The total effective rate of the observation group(98.0%) was significantly higher than that of the control group(90.0%)(P<0.01). Compared with those before treatment, the blood and urine routine, electrocardiogram, liver and kidney function were not significantly changed in both of the two groups after treatment. No other obvious adverse reactions occurred in the two groups after treatment. Conclusion　Gzhi′s Lumbago Prescription is safe and effectively improves the clinical symptoms and signs in the patients with lumbar disc herniation.]]> 2018/9/30 16:15:18 ZHOU Zhen-hui, YANG Zhi-sheng, WEN Jin-hua, et al. 274true ［Abstract］　Objective　To explore the awareness of palliative care among undergraduate medical students. Methods　We developed a cross-sectional study in Wenzhou Medical University, using a self-completion questionnaire. This questionnaire included a knowledge part and an attitude part. 345 students were enrolled in this survey. Results　Higher knowledge scores were associated with being female(P=0.000), being in the third year of study(P=0.009) and having experience of clinical practice(P=0.000). The undergraduate medical students who were in the third year of study, and had experience of clinical practice and higher knowledge scores reported that they had more positive attitudes towards palliative care. Conclusion　Undergraduate medical students′ attitudes towards palliative care are mostly positive but their willingness to involve in palliative voluntary activities is not strong. For school education, interventions require further development to foster better knowledge of palliative care.]]> 2018/9/30 16:15:18 HUANG Bin-ge, CHEN Luo-wei, FANG Si-zhe, et al. 273true ［Abstract］　Objective　To detect the pathogenetic genes of a hypospadias family using exon sequencing(ES) and Sanger sequencing for prenatal diagnosis. Methods　The peripheral blood was collected from probands and their parents, and DNAs were extracted. The pathogenicity-related genes were found by ES of the DNAs related to the gonadal disease of the precursor, and the DNAs of the parents′ parents were verified by Sanger sequencing that they were clearly inherited from the parents. DNAs were extracted from the amniotic fluid samples of the proband mother again, and the Sanger gene was used for prenatal diagnosis. Results　A compound heterozygous mutation of SRD5A2(NM_000348): c.607G>A, P.(Gly203Ser) and c.680G>A, P.(Arg227Gln) heterozygous mutation were found in the proband. The normal phenotype of the mother was SRD5A2(NM_000348): c.607G>A, P.(Gly203Ser) heterozygous mutation, and the normal phenotype of the parent was SRD5A2(NM_000348): c.680G>A, P.(Arg227Gln) heterozygous mutation. The prenatal diagnosis of the proband mother was SRD5A2(NM_000348): c.680G>A and P.(Arg227Gln) heterozygous mutation. Conclusion　ES combined with Sanger sequencing can be used to detect the pathogenic genes to diagnose families hypospadias prenatally and the method is fast, accurate and economical.]]> 2018/8/24 16:25:15 YANG Dong-yan, LIU Li-ling, PANG Li-hong 272true ［Abstract］　Objective　To observe the clinical effect of Yishen Xiaotong pills combined with extracorporeal shock wave on treatment of femoral head necrosis on early or medium-term stage caused by liver and kidney insufficiency. Methods　50 cases(79 hips) of osteonecrosis of the femoral head on the early or medium-term stage caused by liver and kidney insufficiency were selected from the Department of Arthrology, Henan Province Hospital of Traditional Chinese Medicine. According to the random number table, 50 cases were divided into two groups: treatment group(25 cases, 41 hips) and control group(25 cases, 38 hips). The treatment group was treated with Yishen Xiaotong pills combined with extracorporeal shock wave therapy, while the control group was treated with routine western medicine combined with extracorporeal shock wave therapy. TheVisual Analogue Score(VAS) and the hip joint Harris scale(Harris) were recorded to evaluate the clinical efficacy of the two groups before and after treatment. X-ray and MRI were performed on the patients before and after treatment and 6 months after treatment. Results　After treatment, the effect of the treatment group was better than that of the control group(Z=-2.154, P=0.031). The total effective rate was 92.68% in the treatment group and 76.32% in the control group. The VAS scores and Harris scores were improved in both of the two groups after treatment(P<0.05). However, the improvement of the VAS scores and Harris scores in the treatment group were better than those in the control group(P<0.05). Conclusion　Yishen Xiaotong pills combined with extracorporeal shock wave has good clinical effect on the treatment of femoral head necrosis on early or medium-term stage caused by liver and kidney insufficiency. It can prevent the further development of femoral head necrosis or delay the time of collapse of femoral head necrosis.]]> 2018/8/24 16:25:15 YU Peng, WU Sheng-chao, WANG Shang-zeng 271true ［Abstract］　Objective　To explore the techniques for finding and removing the parathyroid glands in surgical treatment for secondary hyperparathyroidism (SHPT). Methods　The case data of 100 SHPT patients who underwent surgical treatment in our department from August 2014 to August 2017 were retrospectively analyzed and the experience and techniques of finding and excising the parathyroid glands were summarized. Results　380 lesions of the parathyroid glands were excised in 100 cases and the results were confirmed by pathology. Of the 380 lesions, 4 parathyroid glands were in 85 cases( 85.0%), <4 parathyroid glands in 11 cases(11.0%), >4 parathyroid glands in 4 cases（4.0%). The upper parathyroid glands were 200 which mainly located in the region of posterior suspensory ligament of the thyroid glands(44.74%) and at the branches of inferior thyroid artery(7.89%). The inferior parathyroid glands were 140 which were located at the bottom of the thyroid gland(31.58%), on the outside of lower pole(5.26%), in the lingual lobe of thymus(7.89%), and by the side of the trachea and the carotid artery(2.63%). In addition to the ectopic parathyroid glands and the parathyroid glands outside of the thyroid glands, a total of 340 parathyroid glands have a relationship with the recurrent laryngeal nerve. Of the 200 upper parathyroid glands, 136 were located in the outside of the recurrent laryngeal nerve(68%). Of the 140 inferior parathyroid glands, 100 were located in the inside of the recurrent laryngeal nerve(71.42%). Conclusion　The indispensable steps of finding the parathyroid glands include knowing well the features of parathyroid anatomical characteristics and the distribution characteristics of “three areas” of parathyroid glands. The use of “search, confirm and protect” three steps can avoid recurrent laryngeal nerve injury during the operation. The local injection of nano carbon suspension into the thyroid glands for finding the parathyroid glands during operation can quickly distinguish the thyroid glands from the peripheral lymph nodes and the parathyroid glands. The joint use of multiple imaging for localization diagnosis can effectively assist SHPT surgery in finding and excising the parathyroid glands.]]> 2018/8/24 16:25:15 LIAO Dan, ZHENG Hou-pu, ZENG Lin, et al. 270true ［Abstract］　Objective　To investigate the relationship between the levels of cerebrospinal fluid lactic acid(CSF-LA) in infantile bacterial pneumonia and the severity of the disease and the complications of nervous system infection. Methods　89 infants with bacterial pneumonia aged from 2 months to 5 years were collected in our hospital during January 2015 and January 2018 and their clinical data were retrospectively analyzed. The patients were divided into the common pneumonia group(n=26) and severe pneumonia group(n=63). The infants with severe pneumonia were divided into the bacterial meningitis(BM) subgroup(n=26) and the non-BM subgroup(n=37). Other 29 non-infectious infants without febrile convulsions aged from 2 months to 5 years were taken as the control group(n=29). The levels of CSF-LA were detected and compared among the groups. Results　The levels of CSF-LA in the severe bacterial pneumonia group were significantly higher than those in the common bacterial pneumonia group and the control group(P<0.05). The levels of CSF-LA in the BM subgroup were significantly higher than those in the non-BM subgroup, the common bacterial pneumonia group and the control group(P<0.01). Conclusion　The levels of CSF-LA can indirectly reflect the severity of bacterial pneumonia in infants and contribute to the diagnosis of infantile bacterial pneumonia complicated with the infection of the nervous system.]]> 2018/8/24 16:25:15 XU Zhi-cheng, XIE Qing-ling, LU Yuan-feng, et al. 269true ［Abstract］　Objective　To observe the effects of Jianpibushen prescription on the expressions of ki67 and P53 in the patients receiving neoadjuvant chemotherapy for triple negative breast cancer. Methods　60 patients with triple negative breast cancer treated in our hospital were selected and were randomly divided into the treatment group(receiving Jianpibushen prescription+TEC, n=30) and the control group(receiving TEC alone, n=30). The short-term clinical effect, the expressions of ki67 and P53 in the breast cancer specimens were observed and compared between the two groups before and after treatment. Results　The short-term clinical effect of the treatment group was better than that of the control group（P<0.05）. The expressions of ki67 and P53 in the treatment group were lower than those of the control group, but there were no significant differences between the two groups（P>0.05）. Conclusion　Jianpibushen prescription has a better effect on triple negative breast cancer. It can reduce the expressions of ki67 and P53 in the cancer tissues.]]> 2018/8/24 16:25:15 LI Juan-juan, LIANG Li-chun, LUO Lin-chuan 268true ［Abstract］　Objective　To discuss the clinical features of Crohn′s disease, and analyze the causes of misdiagnosis, and to provide a reference for correct diagnosis of Crohn′s disease. Methods　The clinical data of 19 cases of Crohn′s disease who were misdiagnosed in our hospital from 2013 to 2018 were retrospectively analyzed. The clinical features, treatment process, and the causes of misdiagnosis were analyzed. Results　The clinical manifestations of 19 cases of Crohn′s disease were not typical. They had been misdiagnosed as chronic enteritis, acute appendicitis, ulcerative colitis, colon cancer, liver cirrhosis and other diseases. Conclusion　The clinical manifestations of Crohn′s disease are diverse. It is necessary to combine the clinical manifestations, endoscopy, pathology and close follow-up of the disease progression to reduce the misdiagnosis and improve the diagnosis rate.]]> 2018/8/24 16:25:15 LI Jing 267true ［Abstract］　Objective　To analyze the clinical characteristics of positive cases of respiratory syncytial virus ribonucleic acid(RSV-RNA) in hospitalized children and to provide the basis for clinical diagnosis and treatment of respiratory syncytial virus(RSV) infection in children. Methods　Oropharyngeal swabs were obtained from the children with respiratory infection symptoms in the People′s Hospital of Yantian District in Shenzhen City during September 2016 and September 2017. RNAs were extracted from the oropharyngeal swabs and the RSV gene was detected by RT-PCR method and the viral load was detected. At the same time, the blood was drawn to detect the routine blood test and C-reactive protein(CRP). Results　There were 114 RSV-RNA positive cases in 1641 children with respiratory infection, and the positive rate was 6.95%. The positive rates of the three groups (<6 months, 6 months～<1 year, 1 year～<2 years) were 14.52%, 10.18% and 10.96%, respectively, which were higher than those of the two groups (3 years～<5 years and ≥5 years)(P<0.05). The positive rate during July and September was 15.01% which was significantly higher than those in the other months(P<0.05). The positive rates were 6.22% and 7.94% in the boys and girls respectively(P>0.05). The leucocyte count of the RSV-RNA positive patients on the high side was 29.82%, on the normal side 65.79%, and on the low side 4.39%. Viral load was not significantly different in each age groups and sex groups(P>0.05), and there was no significant correlation between viral load and leucocyte count, neutrophil ratio, lymphocyte ratio, and CRP(P>0.05). Conclusion　RSV-RNA detection has important value in the diagnosis of children′s respiratory infection. Blood routine and CRP detection provide limited information for virus infection.]]> 2018/8/24 16:25:15 JIN Xian, WU Yi, FAN Chun-hui, et al. 266true ［Abstract］　Objective　To explore the application of intracranial pressure(ICP) monitoring in intracranial puncture and drainage in patients with hypertensive intracerebral hemorrhage. Methods　Ninety-seven patients with hypertensive cerebral hemorrhage received intracranial puncture and drainage in our department between April 2013 and April 2017. They were randomly divided into two groups: observation group(n=50)(receiving ICP monitoring) and control group(n=47)(receiving non-ICP monitoring). The incidence of postoperative complications and Glasco of scores(GOS) were compared between the two groups 6 months after operation. Results　GOS grading of the observation group was better than that of the control group 6 months after operation（P<0.05）. Compared with those in the control group, the incidence rates of pneumonia, stress ulcer and renal dysfunction in the observation group were decreased significantly（P<0.05）. Conclusion　The use of ICP monitoring is safe and effective for intracranial puncture and drainage in the patients with hypertensive intracerebral hemorrhage.]]> 2018/8/24 16:25:15 LU Ming-xiong, LI Ting-yang, HUANG Hai-chang, et al. 265true ［Abstract］　Objective　To investigate the clinical study of acyclovir combined with spleen aminopeptide(oral freeze-dried powder) immunomodulator on infectious mononucleosis(IM) in children. Methods　56 patients with IM were randomly divided into two groups, with 28 cases in each group. The control group was treated with acyclovir for antivirus and symptomatic treatment for 2 weeks. The observation group was treated with a cellular immunomodulator spleen aminopeptide plus the same treatment as the control group. The clinical effect, improvement of clinical symptoms and the changes of T lymphocyte subsets were compared between the two groups. Results　The clinical effect of the observation group was better than that of the control group（P<0.05）. The time for improvement of the clinical symptoms and the signs recovery time, and the time for hospital stay in the observation group was significantly shorter than that in the control group(P<0.05). Compared with those before treatment, the levels of CD3⁺ cells and CD8⁺ cells were significantly lower and the levels of CD4⁺ cells and CD4⁺/ CD8⁺ were significantly higher in both of the two groups after treatment(P<0.05), however the improvements of these indexes in the observation group were better than those in the control group(P<0.05). Conclusion　Acyclovir combined with spleen aminopeptide is safer and more effective than acyclovir alone in treatment of IM in children.]]> 2018/8/24 16:25:15 GUO Yu, HUANG Dong-hui, CAI Wei-xia 264true ［Abstract］　Objective　To construct a clinical prediction rule(CPR) for hand-foot-mouth disease(HFMD) complicated with early neurological involvement. Methods　The clinical data of 1 132 children with HFMD diagnosed were collected in the Children′s Hospital Affiliated to Zhengzhou University from January 2014 to February 2017. R Software Version 3.4.4 was used to analyze the data and the corresponding predictive variables were screened to construct a CPR for HFMD. ROC curve and calibration curve were used to evaluate the performance of the CPR. Results　All the included cases were divided into mild group(n=695) and central nervous system(CNS) lesion group(n=437) depending on whether they had neurological involvement or not. The final constructed CPR included four independent risk factors: peak temperature ≥38.5 ℃, fever duration ≥3 days, WBC≥10.8×10⁹/L, age<1 year(or 2 to 3 years old). The area under the diagnostic ROC curve was 0.821, with a sensitivity of 89.2%, a specificity of 80.7% and a cutoff value of 4 points. Conclusion　The CPR constructed in this study has the advantages of being simple, practical and intuitive, and has a certain reference value for follow-up research and clinical practice.]]> 2018/8/24 16:25:15 HUANG Ping, FENG Hui-fen, WANG Fang, et al. 263true ［Abstract］　Objective　To investigate the differences between acute exacerbation of chronic obstructive pulmonary disease(AECOPD) and chronic obstructive pulmonary disease（COPD） complicated with community acquired pneumonia(CAP). Methods　The clinical data of COPD patiens were collected in our department from January 2013 to August 2015. According to the results of case history and chest X-ray examinations, all the patients were divided into two groups: AECOPD group and COPD complicated with CAP（COPD-CAP） group. The age, white blood cell count, hemoglobin（HB）, albumin(ALB) and inflammatory markers and the proportion of neutrophils,body mass index(BMI), lung function, C-reactive protein(CRP), blood gas analysis and the in-hospital mortality were compared between the two groups. Results　251 COPD patients were included in the study, including 157 AECOPD patients and 94 COPD-CAP patients. The in-hospital mortality of the COPD-CAP group was higher than that of the AECOPD group(OR=3.13, 95%CI=1.19～8.26). The average age of the COPD-CAP group was older than that of the AECOPD group［(78.0±8.3) vs (73.7± 9.4)years, P<0. 001］. The proportion of neutrophils and the levels of CRP and PaCO₂ in the COPD-CAP group were higher than those in the AECOPD group. The levels of BMI, ALB, HB and PaO₂ in the COPD-CAP group were lower than those in the AECOPD group. The COPD-CAP group had more patients coexisting with coronary heart disease. There were no differences between the two groups in smoking status and lung function. Conclusion　The patients with COPD complicated with CAP are prone to have poorer nutritional status, higher levels of inflammatory markers, more complications of coronary heart disease, poorer blood gas analysis and higher in-hospital mortality in those with AECOPD.]]> 2018/7/30 16:15:14 HU Guo-ping, LI Yu-qun, WU Ze-long, et al. 262true ［Abstract］　Objective　To compare the efficacy of a levonorgestrel-releasing intrauterine system(LNG-IUS) and a low-dose combined oral contraceptive(COC) in reducing adenomyosis-related pain and bleeding. Methods　Sixty-two patients with adenomyosis complained of pain and bleeding that were associated with adenomyosis were randomly divided into LNG-IUS group and COC group. The outcomes included the improvement of pain release using a visual analogue scale, menstrual blood loss using a menstrual diary and estimated uterine volume by ultrasound were compared before and 6 months after treatment. Results　The adenomyosis-related pain was relieved obviously and the adenomyosis-related bleeding was remarkably reduced and the sizes of uterine were decreased obviously in both of the two groups 6 months after treatment（P<0.01）, however the improvements of the pain release and bleeding in LNG-IUS group were better than those in COC group（P<0.01）. Conclusion　Both LNG-IUS and COC can relieve the adenomyosis-related pain and reduce the menstrual bleeding. However, LNG-IUS is more effective than COC for treatment of adenomyosis.]]> 2018/7/30 16:15:14 HUANG Li-dong, WU Yu-ying, CHEN Chang-yi, et al. 261true ［Abstract］　Objective　To study the effects of anticoagulants on maternal and fetal outcomes of hypertensive disorders in pregnancy (early onset preeclampsia and chronic hypertension complicated with preeclampsia). Methods　A total of 60 inpatients from the Department of Obstetrics of the First Hospital Affiliated to Guangxi Medical University were randomly divided into two groups during January 2014 and December 2016, with 30 cases in each group. The control group received conventional treatments and the observation group was given anticoagulant treatment（oral aspirin tablet, 50～75 mg/d for 14～28 d plus nadroparin calcium hypodermic injection, 4 100 U/d for 7～10 d） starting at 12 to 14 weeks of pregnancy. The maternal and fetal outcomes were compared between the two groups. Results　There was no significant difference in blood coagulation function between the two groups before and one month after treatment（P>0.05）. The observation group was superior to the control group in the improvements of abnormal umbilical blood flow, fetal growth restriction, reduction of fetal distress and neonatal asphyxia（P<0.05）, but the complications of hypertension during pregnancy were not increased in the observation group including HELLP syndrome, postpartum hemorrhage, placental abruption, cesarean section rate, premature birth rate and perinatal mortality rate（P>0.05）. 6 cases with a history of preeclampsia in the observation group had longer gestational weeks of hypertensive disorders in pregnancy and termination of pregnancy than those they had during the previous pregnancy. Conclusion　(1)Anticoagulants are feasible for preeclampsia and chronic hypertension complicated with preeclampsia, and they can prolong gestational weeks and improve the pregnancy outcomes. (2)Oral aspirin tablet(50～75 mg/d for 14～28 d) can prolong the gestational weeks for the early pregnancy women(12 weeks of pregnancy) with a history of preeclampsia. (3)Oral aspirin tablet in a safe dose（50～100 mg） does not increase the risk of postpartum hemorrhage, placental abruption and teratogenesis.]]> 2018/7/30 16:15:14 WU Fang, WANG Su-mei, GE Li-ping 260true ［Abstract］　Objective　To investigate the correlation between apparent diffusion coefficient(ADC) derived from diffusion-weighted imaging(DWI) and the pathological feature and degree of cervical cancer. Methods　Conventional MRI(magnetic resonance imaging) and DWI(the range of diffusion factor from 0 to 1 000 s /mm²) were performed in forty-three patients with advanced cervical cancer, and the ADC values of them were measured. The correlation was analyzed between ADC and the pathological feature and degree of cervical cancer. Results　There were statistic differences in the ADC values between squamous carcinoma (0.78±0.09)×10^-3 mm²/s and adenocarcinoma (0.94±0.14)×10^-3 mm²/s of cervical cancer(P<0. 05). There was a negative correlation between ADC values of cervical cancer and grades of the tumor(r=-0.676, P=0.008). Conclusion　ADC values may be an ideal tool to evaluate the pathological feature and degree of cervical cancer.]]> 2018/7/30 16:15:14 HUANG Wen-liang, WANG Zhi-yong, FU Sheng-qi, et al. 259true ［Abstract］　Objective　To investigate the relationship between pro-brain natriuretic peptide(pro-BNP) and acute lung injury(ALI) and acute respiratory distress syndrome(ARDS) in children. Methods　30 children with ALI and ARDS who were admitted to our hospital from March 2015 to July 2016 were selected as the observation group. The observation group was divided into three subgroups: A(low level subgroup), B(medium level subgroup) and C(high level subgroup) by the different plasma pro-BNP levels, At the same time, 30 patients with heart disease complicated with dyspnea were selected as the control group. The levels of pro-BNP and the scores of lung injury(LIS) and disease severity(PCIS) were compared between the observation group and the control group. The correlation between pro-BNP and acute lung injury, acute respiratory distress syndrome and the factors of death were analyzed. Results　The serum level of pro-BNP in the observation group was significantly higher than that in the control group(P<0.05), while the level of pro-BNP in the cardiac disease children with pulmonary edema in the control group were higher than that in the observation group(P<0.05). The level of pro-BNP in the children of the observation group who were dead, and whose PCIS scores ≤70 and LIS scores >2.5 was high. The LIS scores of the observation group and one-month survival rate were positively correlated with the level of serum pro-BNP, while PCIS scores were negatively correlated with the level of serum pro-BNP. The pro-BNP levels in B and C intervals were the factors of death in the children with ALI/ARDS. Conclusion　Pro-BNP can evaluate the severity of illness and the prognosis, and provide reference for early diagnosis and treatment in the patients with ALI and ARDS.]]> 2018/7/30 16:15:14 WANG Min 258true ［Abstract］　Objective　To implement the internet-based health management and evaluate its effect on hypertension, and explore the strengthens, weaknesses, opportunities and threats of the management using SWOT analysis. Methods　100 patients with hypertension were managed by internet-based technology and the effect was evaluated 1 month after management. SWOT analysis was performed to explore the strengthens, weaknesses, opportunities and threats of the management model. Results　After one-month intervention, the average blood pressure of the hypertension patients decreased from 135.94/81.88 mmHg to 127.1/78.7 mmHg. The control rate of blood pressure increased from 53.0% to 74.0%. Drug compliance rate increased from 53.2% to 92.2%. SWOT analysis showed that there were obvious advantages for internet-based health management to hypertension. However, it was difficult to manage all the patients with high blood pressure because of the need of the network technology and hardware for the management model. The current status of chronic disease brought opportunities and threats for internet-based health management in China. Conclusion　Internet-based health management for hypertension is effective. It′s worthy of further application and promotion to improve the management effect of hypertension in China.]]> 2018/7/30 16:15:14 ZHOU Zhi-heng, ZHANG Xue-jiao, ZHOU Ya, et al. 257true ［Abstract］　Objective　To analyze the surgical outcomes of percutaneous dilational tracheotomy(PDT) and operational tracheostomy(OT). Methods　398 patients undergoing tracheotomy in our critical-care unit from October 2008 to September 2016 were investigated. All the patients were divided into the normal tracheotomy group, the relatively difficult tracheotomy group and the difficult tracheotomy group according to their different neck dissections, and each group was further divided into group A and group B according to different surgical methods. Group A was treated with PDT, and group B with OT. The surgical methods were chosen by sequential method. The operation time, incision size, bleeding volume during operation, wound healing time and postoperative complications were compared between group A and group B. Results　In the normal tracheotomy group, the relatively difficult tracheotomy group and the difficult tracheotomy group, the operation time, incision size, bleeding volume during operation and wound healing time in group A were significantly shorter or smaller than those in group B(P<0.05). In the normal tracheotomy group and the relatively difficult tracheotomy group, the total incidence rates of postoperative complications in group A(6.2% and 10.3%) were significantly lower than those in group B(65.6% and 76.9%)(P<0.05), but in the difficult tracheotomy group, there was no significant difference between group A and group B(P>0.05). Conclusion　For the common tracheotomy operation, PDT is simpler, quicker and has smaller trauma than OT. For the difficult tracheotomy operation, PDT cannot replace OT, however, in some cases, performing surgical operation based on PDT technology is safer than single surgical incision because of significantly reducing the operation difficulty and risk.]]> 2018/7/30 16:15:14 YANG Zhi-yong, DENG Yan-sheng, LIN Kang-yue 256true ［Abstract］　Objective　To evaluate the changes of cellular immunity in severe hand-foot-mouth disease(HFMD). Methods　Databases including CNKI,VIP, CBM, WanFang Data, PubMed, Medline, Web of Science, Ovid and The Cochrane Library were searched to collect the data about the changes of cellular immunity in severe HFMD. The changes of cellular immunity in different stages were compared. Results　A total of 42 literatures were included. (1)CD4⁺ cells and CD8⁺ cells in severe the HFMD group were significantly decreased than those in the common HFMD group(P<0.05); CD4⁺ and CD8⁺ cells in the severe group and the common group were significantly decreased than those in the health control group (P<0.05). (2) B lymphocyte in the severe group was significantly increased than that in the common group(P<0.05); B lymphocytes in the severe group and the common group were significantly increased than those in the health control group（P<0.05）. (3)There were no significant differences in NK cells between the HFMD group and the control group(P>0.05). Conclusion　The function of T lymphocyte immunity in children with HFMD is in a state of inhibition which is related to the severity of the illness.]]> 2018/7/30 16:15:14 LI Xing-zhi, WU Shu-yue, XIE Qing-ling 255true ［Abstract］　Objective　To explore the clinical effects of post-abortion care(PAC) on induced abortion. Methods　From August 2015 to August 2017, 400 cases of pregnant women who voluntarily required abortion in our hospital received PAC(the observation group). Other matched 400 cases of pregnant women received regular guidance(the control group). The clinical effects were compared between the two groups. The main observational indexes including the effective contraceptive measures, and the unintentional pregnancy, the recurrent miscarriage, and the postoperative complication rate at postoperative 12 months, SAS and SDS scores were compared between the two groups before and after the operation. Results　The rate of the effective contraceptive measures in the observation group(90.00%) was significantly higher than that in the control group(P<0.01). The rate of the patients who used reliable methods of contraception and the rate of non-contraception in the observation group were significantly lower than those in the control group(P<0.01). The rates of unplanned pregnancy, repeated abortion and postoperative complications in the observation group were significantly lower than those in the control group(P<0.05). There were no significant differences in the preoperative SAS and SDS scores between the two groups(P>0.05). The SAS and SDS scores before operation were significantly higher than those after operation in the two groups(P<0.05), but the postoperative scores of the observation group were significantly lower than those of the control group(P<0.01). Conclusion　PAC can improve women′s contraceptive ability, reduce the rates of unplanned pregnancy, repeated abortion and postoperative complications, and therefore improve the psychological status of the women, and have a good clinical application value.]]> 2018/7/30 16:15:14 HE Li, PENG Hua, OU Wen-mei, et al. 254true ［Abstract］　Objective　To investigate the method of culturing primary oral squamous cells from human fresh oral squamous cell carcinoma(OSCC) tissues and to detect the expressions of CD133 and CD44 protein on the surface of squamous cell carcinoma cells by flow cytometry. Methods　(1)Six fresh tumor specimens were taken from the patients who underwent oral squamous cell carcinoma resection from 2016 to 2017 in the Affiliated Stomatological Hospital of Guangxi Medical University. The specimens were treated with anti-contamination to obtain the primary oral squamous carcinoma cells by mechanical method and passaged by enzyme digestion. (2)The primary squamous cell carcinoma cells were identified by cell morphology and immunohistochemical keratin antibody staining. (3)Flow cytometry was used to detect the expressions of CD133 and CD44 on squamous cell carcinoma. Results　(1)The oral squamous carcinoma cells were cultivated, passaged and purified successfully. (2)The OSCCs had a polyhedral shape and displayed a typical paving-stone-like morphology, and keratin staining showed positive brown staining in the cytoplasm. (3)The mean expression rates of CD133 and CD44, surface markers of stem cells in squamous carcinoma cells were 33.76% and 0.41% respectively. Conclusion　A large number of primary oral squamous cell carcinoma cells are successfully obtained from human oral squamous cell carcinoma specimens and successfully passaged. The expression of stem cell surface marker CD44 is relatively high and that of CD133 is relatively low in oral squamous cell carcinoma.]]> 2018/6/29 17:08:36 FANG Fang, MAI Hua-ming, WANG Dai-you, et al. 253true ［Abstract］　Objective　To investigate the changes of interleukin-1β（IL-1β）, interleukin-6(IL-6) and tumour necrosis factor-alpha(TNF-α) and the correlation of the changes with febrile seizures(FS) in children. Methods　72 children with FS were divided into the simple FS(SFS) group(n=40) and the complex FS(CFS) group(n=32). 50 healthy children with matched age and sex were taken as the control group. Enzyme Linked Immunosorbent Assay(ELISA) was used to detect the serum levls of IL-1β, IL-6 and TNF-α. Results　The levels of serum IL-1β, IL-6 and TNF-α in the FS group were all significantly higher than those in the healthy control group(P<0.05). The levels of serum IL-1β, IL-6 and TNF-α in the SFS group were all significantly lower than those in the CFS group(P<0.05). There was a positive correlation between the levels of IL-1β, IL-6 and TNF-α and FS in children(P<0.05). Conclusion　The increased levels of IL-1β, IL-6 and TNF-α are correlated with the attack of FS, and have a synergistic effect on FS.]]> 2018/6/29 17:08:36 CHEN Hao, PAN Yang-Qiong, ZHANG Xiang-Min, et al. 252true ［Abstract］　Objective　To explore the relationship between serum visfatin level and urine albumin-to-creatinine ratio(ACR) in non-obese patients with type 2 diabetes, and to investigate the changes of visfatin in diabetic nephropathy at different stages. Methods　90 patients with type 2 diabetes and body mass index(BMI)<28 kg/m² were divided into 3 groups according to the different urine ACRs: T2DM group(urine ACR<30 μg/mg), DN1 group(urine ACR 30～300 μg/mg) and DN2 group（urine ACR>300 μg/mg). 30 healthy volunteers were collected as the control group. The levels of serum visfatin, glucose and blood lipid, and renal function were compared among the groups. Results　The level of serum visfatin in non-obese patients with type 2 diabetes was significantly higher than that in the control group(P<0.01). The level of serum visfatin in diabetic nephropathy patients was significantly higher than that in simple diabetic patients(P<0.01), and visfatin level in patients with massive albuminuria was higher than that in the patients with microalbuminuria(P<0.01). Serum visfatin and urine ACR levels were positively correlated(r=0.558, P<0.01). Conclusion　The level of serum visfatin is elevated in non-obese patients with type 2 diabetes, and closely related to urine ACR. With the aggravation of diabetic nephropathy, visfatin is gradually increased. Visfatin has the potential to be therapeutic targets for diabetic nephropathy.]]> 2018/6/29 17:08:36 LU Xin-hong, WEN Yu-jie, HU Xin, et al. 251true ［Abstract］　Objective　To discuss the voice analysis and vocal cords observation in early stage laryngeal cancer patients after supported laryngoscopy combined with endoscopic electrocoagulation. Methods　100 patients with early stage laryngeal cancer were selected from January 2012 to August 2016 in our hospital and randomly divided into the observation group and the control group, with 50 cases in each group. The control group received laryngeal fissure vocal cord excision or laryngectomy. The observation group received supported laryngoscopy and endoscopic electrocoagulation of diseased vocal cords. The patients were divided into vocal cord neogenesis group(n=35) and vocal cord non-neogenesis group(n=65) according to different vocal cords. The acoustic characteristics were compared between the two groups. Results　There were no significant differences between the two groups in FO and MPT before operation(P>0.05). FO was lower and MPT was higher in the observation group than those in the control group 2 weeks, 1 and 3 months after surgery(P<0.05). The rate of vocal cord neogenesis in the observation group(58.00%) was higher than that in the control group(12.00%). FO was higher and MPT was lower in the vocal cord non-neogenesis group than in the vocal cord neogenesis group(P<0.05). Conclusion　Supported laryngoscopy combined with endoscopic electrocoagulation causes small lesions and is conducive to regenerating the vocal cords for the patients with early stage laryngeal cancer.]]> 2018/6/29 17:08:36 CHEN Jin-xian, LIN Chu-biao, CHEN Wei-long 250true ［Abstract］　Objective　To investigate the effect of clinical pathway management on surgical outcomes. Methods　A total of 358 patients who were implemented clinical pathway management including 92 cases of planned cesarean section, 71 cases of ureteral calculus, 64 cases of uterine leiomyoma, 53 cases of inguinal hernia and 78 cases of acute appendicitis were randomly collected as the observation group in our hospital from September 2014 to September 2016. Other 217 patients who were not implemented clinical path management including 56 cases of planned cesarean section, 43 cases of ureteral calculus, 39 cases of uterine leiomyoma, 32 cases of inguinal hernia and 47 cases of acute appendicitis were randomly collected as the control group. The average medical costs, drug costs, average hospitalization days and the patients′ satisfaction were compared between the clinical pathway group and non-clinical pathway group for the same diseases. Results　There were significant differences in the average medical costs and drug costs of planned cesarean section, ureteral calculus, leiomyoma of uterus and inguinal hernia disease between the clinical pathway and non-clinical pathway groups(P<0.05). There were significant differences in the average hospitalization days of the five diseases between the clinical pathway and non-clinical pathway groups(P<0.05). There were significant differences in the patients′ satisfactions of planned cesarean section, ureteral calculus, leiomyoma of uterus and acute appendicitis between the clinical pathway and non-clinical pathway groups(P<0.05). Conclusion　Clinical pathway management can not only significantly reduce the average medical costs, drug costs and average hospitalization days but also significantly improve the patients′ satisfaction.]]> 2018/6/29 17:08:36 XIAO Min, CAI Le, LU Jun-li, et al. 249true ［Abstract］　Objective　To explore the effect of special electromagnetic wave(Te-ding Dian-ci-bo Pu, TDP) plus low-molecular-weight heparins calcium on preventing venous thrombosis in the patients with malignant tumors after peripherally inserted central catheter(PICC). Methods　503 patients with malignant tumors who received PICC were randomly divided into the observation group(n=249) and the control group(n=254). The observation group was treated with subcutaneous injection of low-molecular-weight heparins calcium plus TDP. The control group was treated with low-molecular-weight heparins calcium. The incidence of venous thrombosis was compared between the two groups. Results　The incidence of venous thrombosis in the observation group(7.23%) was significantly lower than that in the control group(13.78%)（P=0.017）. Conclusion　TDP plus low-molecular-weight heparins calcium can reduce the incidence of venous thrombosis in the patients with malignant tumors after PICC.]]> 2018/6/29 17:08:36 LAN Hai-yan, FENG Guo-sheng, HAO Yan-rong, et al. 248true ［Abstract］　Objective　To study the clinical effectiveness of phloroglucinol in treatment of vaginal bleeding after in-vitro fertilization and embryo transfer. Methods　84 patients suffered from vaginal bleeding and abdominal pain after in-vitro fertilization and embryo transfer were collected in the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2015 to January 2017 and were randomly divided into the control group and the research group, with 42 cases in each group. The patients in the control group were treated with progesterone alone, and those in the research group were treated with progesterone and phloroglucinol simultaneously. The adverse reactions, the improvements of clinical symptoms such as vaginal bleeding and abdominal pain were observed and compared between the two groups. The pregnancy outcomes such as abortion rate, live birth rate and neonatal malformation rate were compared between the two groups. Results　The improvements of vaginal bleeding and abdominal pain in the research group were better than those in the control group（P<0.05）. No adverse reactions were observed in both of the two groups. There were no significant differences in the abortion rate and live birth rate between the two groups（P>0.05）. The malformed newborn was not founded in both of the two groups. Conclusion　Phloroglucinol is safe and effective in improving the clinical symptoms of vaginal bleeding after in-vitro fertilization and embryo transfer.]]> 2018/6/29 17:08:36 CHI Yan, QIN Jie, ZHANG Yi-sheng, et al. 247true ［Abstract］　Objective　To compare the expressions of interleukin-35(IL-35) in peripheral blood of the patients with different pathological types of primary liver cancer. Methods　52 patients with hepatitis B virus(HBV) associated hepatocellular carcinoma(HCC) were collected in the First Affiliated Hospital of Guangxi Medical University from January 2012 to January 2013. 12 patients with benign liver tumors were enrolled as the control group and 6 patients with intrahepatic cholangiocarcinoma(ICC) and 5 patients with mixed liver cancer were also enrolled. The expression of IL-35 in plasma was detected by enzyme-linked immuno sorbent assay(ELISA) method. Results　The expressions of IL-35 were greatly higher in the HCC group, ICC group and mixed liver cancer group compared with those in the control group（P<0.05）. The expression of IL-35 was greatly higher in the HCC group compared with that in the ICC group（P<0.05）. There was no significant difference in the expression level of IL-35 between the mixed liver cancer group and the ICC group（P>0.05）. Conclusion　IL-35 is highly expressed in HCC, and may be a molecular marker for differentiating the different pathological types of primary liver cancer.]]> 2018/6/29 17:08:36 SONG Jing-qing, YAO Yin-long, XU Bang-hao, et al. 246true ［Abstract］　Objective　To study the prediction and clinical significance of radiosensitivity gene of miR-93-5p in rectal cancer.Methods　The data of rectal cancer treated by radiotherapy were collected from the GEO database. Limma.R package software was used to calculate the differentially expressed genes to obtain the first gene set. The target gene of miR-93-5p in multiple micro-RNA target gene prediction database was used to predict and obtain the additional gene set. Plastin 1(PLS1) gene is achieved by intersecting two gene sets. Then survival analysis and gene set enrichment analysis of the expression level of PLS1 were performed to explore the clinical significance of molecular mechanisms of PLS1.Results　The total survival time of the patients with higher-level expression of PLS1 was obviously shorter than that of the patients with low-expression PLS1 gene（P=0.01）.Conclusion　Higher-level expression of PLS1 gene is a bad prognostic factor for rectal cancer and can be used as an effective biomarker to predict the prognosis of the patients.]]> 2018/5/30 8:21:22 CHEN Liang, ZHANG Hong-yan 245true ［Abstract］　Objective　To explore the mechanism of antiangiogenesis and the evaluation of perioperative liver function in primary hepatocellular carcinoma in Guangxi.Methods　The survival data of liver cancer patients were analyzed by molecular biology experiment, animal experiment and drug resistance experiment. A linear regression analysis was performed to observe the acoustic radiation force impulse(ARFI) score and postoperative standard residual liver volume(SRLV) in 104 patients with liver cancer. Preoperative urinary 8- hydroxydeoxyguanosine (8-OHdG) and peripheral blood T lymphocyte subgroup CD4⁺ and CD8⁺ expressions were detected.Results　COX-2/PGE2 signaling pathways regulated VEGF activity via regulating HIF-2 α activity; PEG-SS-PLL effectively transfected the siVEGF, and its cytotoxicity was negligible, and the expressions of VEGF in mRNA and the protein levels significantly decreased both in vitro and in vivo, inhibiting tumor growth. The critical value of SRLV with severe compensation was 503 ml/m². The linear regression equation of preoperative ARFI score and postoperative SRLV was: SRLV(ml/m²)=149.6×ARFI score(m/s)+194.1. Preoperative urinary 8-OHdG level was significantly higher than that of the control group ［(16.15±4.96)ng/mg Cr vs (12.13±4.66)ng/mg Cr］.Conclusion　The specific inhibitors of COX-2 /PGE2 pathway, meloxicam or celecoxib can significantly enhance the drug sensitivity of liver cancer molecular targeted drug sorafenib. PEG-SS-PLL/siVEGF may be applied to the anti-angiogenesis gene therapy of hepatocellular carcinoma. Combining SRLV with the detection of hepatic fibrosis degress has important guiding value for the primary hepatocellular carcinoma patients receiving preoperative assessment of the amount of liver resection. For the cases of medium or severe liver fibrosis, the safety of SRLV threshold is 503 ml/m². Urinary 8-OHdG has high application value in the diagnosis and treatment of liver cancer.]]> 2018/5/30 8:21:22 TANG Zhen-yong, DONG Xiao-feng, YANG Jian-rong, et al. 244true ［Abstract］　Objective　To explore the clinical effect of 3D printing technology on treatment of complex tibial plateau fractures.Methods　38 inpatients with complex tibial plateau fractures treated in our hospital between July 2013 and May 2016 were collected as the research subjects. All the patients had fresh and closed fractures(Schazker classification: Ⅴand Ⅵ types) and were randomly divided into two groups. Before surgery, the experimental group used Mimics software to process the fractured CT scan data and printed the 1∶1 size tibial plateau model. According to the bone models, the appropriate surgical approaches, reduction methods and internal fixations were chosen. The control group used the traditional method: open reduction and internal fixation. The operation time, intraoperative bleeding, The Hospital for Specical Surgery Knee-ralting Score(HSS) and The MOS item short from health survey(SF-36) were recorded 1 year after operation.Results　The 38 patients were followed up for more than 1 year. All surgical incision wounds healed in grade A. The fracture healing time was 10 to 16 weeks［average (14.5±2.1)weeks］. The operation time was （82.4±11.2）min and （126.5±9.1）min in the experimental group and the control group respectively. The intraoperative bleeding volume was （88±39）ml and（302±49）ml in the experimental group and the control group respectively. Compared with the control group, the experimental group had shorter operation time and less bleeding volume(P <0.05). There were no significant differences between the experimental group and the control group in HSS［(91.3±2.8) vs (90.1±3.6)］ and SF-36 scores［(89.1±5.6) vs (90.7±7.8)］(P>0.05).Conclusion　3D printing technology can greatly shorten the operation time, reduce the intraoperative bleeding and promote the postoperative knee function recovery in adjuvant treatment for complex tibial plateau fractures.]]> 2018/5/30 8:21:22 ZHAO Ning, CHEN Jing, ZHONG Hua, et al. 243true ［Abstract］　Objective　To investigate the effects of different degrees of gestational hypertension on delivery outcomes.Methods　The data of 150 patients with different degrees of gestational hypertension in our hospital during February 2016 and January 2017 were retrospectively analyzed. The patients were divided into three groups according to the different degrees of gestational hypertension: mild group(with gestational hypertension, n=71), moderate group(with mild preeclampsia, n=41) and severe group(with severe preeclampsia, n=38). The rates of adverse pregnancy outcomes were compared among the three groups.Results　There was no significant difference in maternal age among the three groups(P>0.05). The gestational age and newborn weight decreased and the incidence rates of intrauterine distress, neonatal asphyxia and intrauterine growth restriction increased in the mild group, the moderate group and the severe group in accordance with the severity of gestational hypertension(P<0.05). The incidence rates of placental abruption increased in the three groups in accordance with the severity of gestational hypertension(P<0.05). The incidence of heart failure in the mild group was significantly lower than that in the severe group(P<0.05).Conclusion　The adverse outcomes of the fetuses, newborns and parturient women increase with the severity of gestational hypertension.]]> 2018/5/30 8:21:22 XU Zhi-li, FENG Min-qing, LIU Dan, et al. 242true ［Abstract］　Objective　To compare the changes of umbilical cord blood flow parameters and the middle cerebral artery peak systolic velocity before and after the amniotic infusion therapy, and to explore the effect of amniotic infusion therapy on fetal umbilical cord blood flow and middle cerebral artery peak systolic velocity（MCA-PSV） in oligohydramnios.Methods　18 pregnant women who had been diagnosed with oligohydramnios by sonography between 22 and 33 gestational weeks received amniotic infusion in our department from January 2014 to December 2016. The amniotic fluid index(AI), umbilical blood flow parameters such as S/D, RI and middle cerebral artery peak systolic velocity(MCA-PSV) were recorded and compared before and after amniotic infusion.Results　The amniotic fluid index was improved significantly(P<0.01), S/D and RI were both decreased significantly(P<0.01, P<0.05) after amniotic infusion, but MCA-PSV was not markedly changed. Conclusion　Amniotic infusion therapy can improve amniotic fluid index and umbilical artery flow parameters in a short period, but has no effect on the peak systolic velocity of the middle cerebral artery.]]> 2018/5/30 8:21:22 QIN Ting, TIAN Mao, ZHANG Ji-hong, et al. 241true ［Abstract］　Objective　To observe the effects of advanced epidural cavity catheter analgesia on delivery modes and outcomes.Methods　120 parturient women with epidural labor were divided into group A and group B, with 60 cases in each group. In group A, there were regular contractions, orifice of the uterus <3 cm, and the epidural catheter was used to relieve pain when the women were unable to endure delivery pain. In group B, there were regular contractions, orifice of the uterus ≥3 cm, and the epidural catheter was inserted into the catheter and analgesia was immediately administered. The effects of analgesia were compared between the two groups. Results　Both of group A and group B had good delivery analgesia effects, and there were no significant difference between them（P>0.05）. However, group A had more initiative in the timing of analgesia than group B, and the satisfaction degree of delivery anesthesia in group A was higher than that in group B（P<0.05）. There were no significant differences between the two groups in the rate of using oxytocin, the rate of transferring to caesarean section, the time for the first stage of labor, the blooding volume 2 hours after delivery, and the Apgar scores of the newborns in 1 minute （P>0.05）.Conclusion　The analgesic effect of epidural catheterization is safe, reliable and satisfying for the mothers and their newborns.]]> 2018/5/30 8:21:22 XU Yin-li, HE Xue-ming, HE Wen-ping, et al. 240true ［Abstract］　Objective　To investigate the effects of innovation-guided integrated teaching mode in clinic probation teaching of radiotherapy in higher vocational college.Methods　126 students in higher vocational colleges were randomly divided into the control group and the experimental group. The control group received the traditional teaching mode, while the experimental group was trained with the innovation-guided integrated teaching mode. The teaching effects were compared between the two groups on theoretical knowledge, skills test and questionnaire survey.Results　There was no significant difference between two groups in theoretical knowledge. The experimental group had significantly higher scores than the control group in case analysis test, skills test and questionnaire survey about learning interest, information search, problem solving, innovative thinking, communication and cooperation competence(P<0.05).Conclusion　Innovation-guided integrated teaching mode not only promotes the students′ theories and practice results, but also improves their innovation consciousness, innovative thinking and ability.]]> 2018/5/30 8:21:22 GU Jun-zhao, HUANG Yi-hang, DENG Shan, et al. 239true ［Abstract］　Objective　To perform the comparative analysis of perineal incision and unprotected perineal childbirth.Methods　A retrospective analysis of 200 cases of vaginal delivery in our hospital from April 2015 to May 2016 was preformed in this study. According to the different methods of delivery that the patients received, they were divided into the perineal incision group and the unprotected group, with 100 cases in each group. The perineal incision group received traditional protection of the perineal method and the unprotected group received unprotected perineum natural vaginal delivery. The degree of perineal rupture and the post-delivery results were compared between the two groups.Results　The degree of perineum of the unprotected group was significantly less than that of the perineal incision group(P<0.05). There were no significant differences in the second delivery time and Apgar scores between the two groups(P>0.05). The postpartum 2 h blood loss of the unprotected group was significantly less than that of the perineal incision group(P<0.05), The maternal satisfaction scores of the unprotected group were significantly higher than those of the perineal incision group(P<0.05).Conclusion　The unprotected perineal childbirth method reduces more degree of perineal fracture and more amount of postpartum 2 h blood loss and improves more maternal satisfaction than the perineal incision method.]]> 2018/5/30 8:21:22 LIN Lan, LIN Wei-ling, XU Shao-min 238true ［Abstract］　Objective　To investigate the effects of eIF4A1 gene silencing on the proliferation, apoptosis, migration of human gastric cancer cell lines and its molecular mechanisms.Methods　Human gastric cancer cell lines SGC-7901 were infected with the recombinant lentivirus vector LV-EIF4A1-RNAi for eIF4A1 gene silencing, as eIF4A1-RNAi group, while control recombinant lentivirus vector CON077 infected with human gastric cancer cell lines SGC-7901, as the negative control group(CON077) and blank control group without any treatment. CCK-8 was used to detect cell proliferation activity, and the cell apoptosis rate and cell cycle distribution were detected by flow cytometry. The activity of migration and invasion of cells were evaluated by Transwell. Quantitative real-time polymerase chain reaction(QRT-PCR) and Western blot were applied for measurement of mRNA and protein expressions of eIF4A1 and AKT.Results　The expression of eIF4A1 gene in LV-EIF4A1-RNAi(44683-1) group was the lowest (0.31±0.04), so the group was selected as eIF4A1 silent group(eIF4A1-RNAi). Compared with those in the CON077 group and the control group, the cell proliferation activity was significantly decreased, the cell apoptosis rate was increased, the cell cycle was blocked in S phase, the G₁/G₂ phase cells were decreased, and the cell migration and invasion ability were decreased in the eIF4A1-RNAi group（P<0.05）. The expressions of mRNA and protein of eIF4A1 and AKT were down-regulated. No significant differences were found in the measurements between the CON077 group and the blank control group（P>0.05）.Conclusion　Lentivirus-mediated eIF4A1 gene silencing inhibits the growth and migration of human gastric cancer cell lines, induces cells apoptosis and arrests cell cycle in S phase, and its mechanisms may be related to the downregulation of AKT expression.]]> 2018/4/27 7:59:26 XIE Dong-yi, WEI Wei-yuan, WU Kun, et al. 237true ［Abstract］　Objective　To analyse the expression of programmed cell death 4 （PDCD4） and telomerase in myocardial tissues of sudden death patients with coronary heart disease and to discuss its clinical significance in coronary heart disease patients with myocardial injury.Methods　25 sudden death patients with coronary heart disease(cardiac sudden cardiac death in coronary heart disease group), 25 non-sudden death patients with coronary heart disease(non- cardiogenic sudden death of coronary heart disease group) and 25 dead patients without coronary heart disease(normal control group) were collected. PDCD4 and telomerase were detected by immunohistochemical method and were analyzed among the three groups.Results　(1)The positive rates of PDCD4 and telomerase in the cardiac sudden cardiac death in coronary heart disease group were 27.36% and 30.08% respectively.The positive rates of PDCD4 and telomerase in the non-cardiogenic sudden death of coronary heart disease group were 73.58% and 76.22% respectively. The positive rates of PDCD4 and telomerase in the normal control group were 89.52% and 92.31% respectively. The positive rates of PDCD4 and telomerase in the sudden cardiac death of coronary heart disease group were significantly lower than those in the other two groups（P<0.05）. (2)The median values of optical density of PDCD4 and telomerase in the cardiac sudden cardiac death in coronary heart disease group were 2 776.32 and 11 889.65 respectively. The median values of optical density in the non-cardiogenic sudden death of coronary heart disease group were 1 073.52 and 7 254.91 respectively. The median values of optical density in the normal control group were 987.48 and 6 908.27 respectively. The median values of optical density of PDCD4 and telomerase in the sudden cardiac death of coronary heart disease group were significantly higher than those in the other two groups（P<0.05）.Conclusion　The positive rates of PDCD4 and telomerase are lower in the cardiac muscle tissues of the patients with CHD cardiac sudden death whose median values are overexpressed, but higher in non-cardiogenic sudden death patients with coronary heart disease and in the dead patients without coronary heart disease. These indicate that PDCD4 and telomerase may be the key markers of myocardial injury in coronary heart disease, and have a certain guiding role in the clinical diagnosis and treatment of coronary heart disease.]]> 2018/4/27 7:59:26 CHEN Gang, WANG Yang, WU Xu-ting, et al. 236true ［Abstract］　Objective　To study the effect of multi-frequency vibration therapy instrument on earlier intestinal rehabilitation of acute gastrointestinal injury(AGI) in critical patients.Methods　Seventy patients with AGI were enrolled in the Intensive Care Unit(ICU) of the People′s Hospital of Guangxi Zhuang Autonomous Region between September 2016 and September 2017, and were divided into observation group(n=35) and control group(n=35) by random number table method. The control group was given conventional therapy. The observation group received the same treatment as the control group plus multi-frequency vibration therapy. The AGI and bowel sound grades, the levels of intra-abdominal pressure, procalcitonin and C- reactive protein, the length of ICU stay, ICU hospitalization expenses and mortality rate during hospitalization were compared between the two groups.Results　There were no significant differences between the two group in the AGI and bowel sound grades 3 days after treatment（P>0.05）. The improvements of the AGI and bowel sound grades in the observation group were better than those in the control group 7 and 28 days after treatment（P<0.05）. After treatment, the levels of intra-abdominal pressure, procalcitonin and C- reactive protein in both of the two groups were significantly reduced than those before treatment（P<0.05）, however the reduction extent of the observation group was significantly larger than that of the control group(P<0.05). The length of ICU stay in the observation group was significantly shorter than that in the control group(P<0.05), but the ICU hospitalization expenses were higher in the observation group(P<0.05). There was no significant difference in the 28-day mortality rate during hospitalization between the two groups(P>0.05).Conclusion　Early use of multi-frequency vibration therapy instrument can effectively improve the gastrointestinal function in the critical patients with AGI.]]> 2018/4/27 7:59:26 ZHU Liang-feng, LYU Li-wen, SHEN Yin, et al. 235true ［Abstract］　Objective　To analyze the effect of epidural labor analgesia on umbilical artery and vein blood gas values.Methods　61 healthy singleton primiparas were chosen and divided into analgesia group and control group according to voluntary principle. The analgesia group(n=36) received epidural analgesia. When the uterine ostium was open to 3 cm, L_2-3 lumbar vertebral space was indwelled epidural catheter with analgesia pump. The control group(n=25) voluntarily gave up any analgesia for labor. 2 ml of umbilical artery and vein blood was drawn respectively for blood gas assay after delivery.Results　The results of umbilical artery and vein blood gas analysis were within the normal ranges in both of the two groups. The level of lactic acid of umbilical artery in the analgesia group was significantly higher than that in the control group(P<0.05). The analgesic total dose showed a negative correlation with the level of lactic acid of umbilical artery(r=-0.553).Conclusion　Epidural labor analgesia has no significant effect on umbilical artery and vein blood gas values, but can reduce the level of lactic acid of umbilical artery, which is beneficial to the neonates.]]> 2018/4/27 7:59:26 ZHANG Yan-peng, YANG Bao-zhong, JING Zi-ye, et al. 234true ［Abstract］　Objective　To investigate the effect of Myofunctional Research Company（MRC） muscle function corrector on treatment of children′s oral bad habits accompanied by malformed teeth.Methods　25 children with oral bad habits accompanied by malformed teeth were collected in our department from March 2011 to March 2014 and were divided into the treatment group(n=13) and the control group(n=12) according to odd days of filing. The treatment group was treated with soft MRC muscle function appliances(mainly the infant type) and the control group received the traditional functional corrector. The effect of correcting oral bad habits and the subjective assessments by the parents′ questionnaires were compared between the two groups.Results　The success rate of the treatment group(76.9%) was higher than that of the control group(66.7%), but there was no significant difference between the two groups(P>0.05). The success rates of wearing a fixed corrector in the treatment group were significantly higher than those in the control group respectively at the first week, 1 month and 3 months of the treatment(P<0.05).Conclusion　The MRC muscle function corrector has advantages of light weight, no damage, low cost and low technical sensitivity and is suitable for the treatment of children′s bad oral habits.]]> 2018/4/27 7:59:26 SHI Chun-mei, OU Xiao-li, ZHOU Yan, et al. 233true ［Abstract］　Objective　To observe the curative effect of biofeedback combined with mosapride in treatment of slow transit constipation(STC).Methods　108 patients with STC were randomly divided into group A(n=38), group B(n=33) and group C(n=37). Group A received biofeedback in combination with mosapride; Group B received mosapride plus virtual biofeedback; Group C was treated with biofeedback plus placebo. The curative effects were compared among the three groups 4 weeks after treatment.Results　The three differentnt regimens were effective to improve the patients′ clinical symptoms and elevate their colon discharge rates. The curative effect of group A was superior to that of group B or group C(χ²=12.914, P=0.002).The number of colon discharge in group A was larger than that of group B or group C(P<0.05).Conclusion　Biofeedback combined with mosapride is more effective in treatment of STC than a single treatment of mosapride or biofeedback therapy. It can obviously accelerate the colon emptying and is worthy of expanding further study.]]> 2018/4/27 7:59:26 MAO Yan-ning, TANG Shao-bo, HUANG Gang-ding 232true ［Abstract］　Objective　To investigate the relationship between body mass index(BMI), uric acid and blood lipid on the elderly of Yao nationality in Gongcheng county, Guangxi Zhuang Autonomous Region.Methods　1 223 elderly community residents of Yao nationality were selected by cluster random sampling. Their height and weight were measured, and the levels of uric acid and lipid were detected, and the correlation between the body mass index, uric acid and dyslipidemia on them was analyzed.Results　The abnormal rate of blood lipid was 29.44%(360/1 223), and the overweight rate was 11.93%(146/1 223) and the prevalence rate of hyperuricemia was 19.71%(241/1 223) in the respondents. The dyslipidemia rate, overweight rate, high uric acid rates of the males were significantly higher than those of the females(34.82% vs 23.74%, 14.31% vs 9.43%, 25.91% vs 13.13%, P<0.05). With the increase of BMI, the prevalence of dyslipidemia increased gradually(P<0.05), and BMI was positively correlated with TG, TC and LDL-C, and negatively correlated with HDL-C. The prevalence of dyslipidemia in the hyperuricemia group was significantly higher than that in the uric acid group(P<0.05), and uric acid was positively correlated with TG, TC and LDL-C, and negatively correlated with HDL-C.Conclusion　The prevalence of dyslipidemia is high in the elderly of Yao nationality in Gongcheng county, Guangxi Zhuang Autonomous Region. Overweight, obesity and hyperuricemia are the risk factors of dyslipidemia in the elderly of Yao nationality.]]> 2018/4/27 7:59:26 CHEN Hui-lan, QIN Cong-jun, HE Zhi-ming, et al. 231true ［Abstract］　Objective　To study the clinical diagnosis and treatment of entreocutaneous fistula.Methods　The clinical data of 149 cases with enterocutaneous fistula, of whom 119 cases were transfered to our hospital from other hospitals during June 2013 and January 2017 were retrospectively analyzed.Results　After comprehensive treatments, 129 cases(86.58%) were successfully cured while 13 cases(8.72%) gave up the treatment and were discharged on request, 7 cases died mainly because of severe sepsis and multiple organ failure. In these patients, abdominal trauma caused intestinal fistula in 18 cases(12.08%), and the other patients suffered from intestinal fistula 1 to 2 weeks after abdominal surgery. All the patients were diagnosed with intestinal fistula through auxiliary imaging examination or abdominal puncture and bacterial culture. Most the patients were cured or improved by the treatments of complete drainage, anti-infection and nutrition support. 8 cases were performed ileum colostomy surgery in the early stage and 12 cases were performed two-stage operation of fistula closed surgery or Intestinal fistula resection+Intestinal fistula resection.Conclusion　Complete drainage in early stage is the key factor for successful treatment and effective anti-infection, nutritional support and vital signs and major organs monitoring are the foundation of a good clinical outcome for the patients with entreocutaneous fistula.]]> 2018/3/28 17:03:21 WANG Long, WANG Xiao-tong, MAI Wei, et al. 230true ［Abstract］　Objective　To investigate the relationship between serum level of homocysteine and SYNTAX score in type 2 diabetic patients with coronary artery disease(CHD).Methods　160 patients with type 2 diabetes were chosen as the research subjects in our hospital between June 2016 and June 2017. 80 healthy people were taken as the control group. According to the coronary arteriography, the 160 patients with type 2 diabetes were divided into two groups: type 2 diabetes without coronary heart disease group(n=80), type 2 diabetes with coronary heart disease group(n=80). According to SYNTAX scores, type 2 diabetes with coronary heart disease group was divided into low risk group(1～22 score, n=23), middle risk group(23～32 score, n=30) and high risk group(≥33 score, n=27).Results　The levels of serum homocysteine in type 2 diabetes group and type 2 diabetes with coronary heart disease group were higher than those in the control group. The levels of serum homocysteine in the type 2 diabetes group were significantly lower than those in type 2 diabetes with coronary heart disease group, and the levels of serum homocysteine in the low risk group and middle risk group were significantly lower than those in the multi-branch lesion group(P<0.05).Conclusion　The level of serum HCY is significantly associated with SYNTAX score in the patients with type 2 diabetes and coronary artery disease, suggesting that the level of serum HCY should be regarded as the reference index of the severity of coronary artery disease.]]> 2018/3/28 17:03:21 WANG Bo-le, WANG Huan-xia, XU Jin-yi, et al. 229true ［Abstract］　Objective　To study the influence of micro blood collection tube on detection of copper（Cu）, zinc（Zn）, calcium（Ca）, magnesium（Mg） and iron（Fe） using atomic absorption spectrometry（AAS）.Methods　80 samples of whole blood were divided into the micro blood sampling group(n=40) and the pipette sampling group(n=40) and they were examined for Cu, Zn, Ca, Mg and Fe by micro blood collection tube and pipette respectively. 40 samples which used micro blood collection tubes were soaked in the diluent, and were taken as the soaking group. Under the same conditions, 40 blank reagents without any samples were taken as the control group. The test results between the micro blood sampling group and the pipette sampling group, the soaking group and the control group were compared.Results　The levels of Zn, Ca and Fe in the soaking group were significantly higher than those in the control group（P<0.01）. Compared with those in the pipette sampling group, the levels of Zn and Ca in the micro blood sampling group were significantly increased（P<0.01）.Conclusion　The results of Zn and Ca in the determination of trace elements are greatly influenced by micro blood collection tubes. It is necessary to set up blank controls using micro blood collection tubes to reduce the impact on the detection of trace elements.]]> 2018/3/28 17:03:21 YANG Wen-hui, LI Wei-ping, NING Le-ping, et al. 228true ［Abstract］　Objective　To investigate the effect of epidural anesthesia combined with unprotected perineum delivery on reducing episiotomy rate.Methods　80 cases of pregnant women who gave normal birth in our hospital from April 2016 to December 2017 were selected as the experimental group, and the experimental group received epidural anesthesia combined with unprotected perineum delivery. 80 cases of pregnant women who gave normal birth in our hospital in the same period were selected as the control group. The control group received traditional block anesthesia. The second stage of labor time, maternal perineal resection rate and perineal laceration were compared between the two groups.Results　The second stage of labor time in the experimental group (31.6±5.4)min was significantly shorter than that in the control group(P<0.05). The maternal perineal resection rate and perineum Ⅱ injury rate in the experimental group were significantly lower than those in the control group. The integrity rate of the experimental group was significantly higher than that of the control group(P<0.05).Conclusion　Compared with the traditional delivery mode, epidural anesthesia combined with unprotected perineum delivery technique can significantly shorten the labor process, reduce the episiotomy rate and perineal laceration degree, and promote postpartum recovery.]]> 2018/3/28 17:03:21 YUAN Hui-hong 227true ［Abstract］　Objective　To observe the clinical efficacy of electromagnetic extracorporeal shockwave lithotripsy(ESWL) in treatment of urethral calculi under B ultrasonic positioning.Methods　The clinical data of 96 patients with urethral calculi who were treated with ESWL in our hospital were retrospectively analyzed. The patients′ positions, B ultrasonic positioning methods and lithotripsy effect were observed and analyzed.Results　When males took low head and high foot posture position, the success rate of removal of posterior urethral calculi was 100%, and the stone clearance rate was 96.9%. When males took a riding position or a semi-sitting position, the success rate of removal of posterior urethral calculi was 100%, and the stone clearance rate was 92.1%. For males with posterior urethral calculi, the success rate of localization of urethral lithiasis was 100% and the stone clearance rate was 100%. For females with urethral calculi, the success rate of localization of urethral lithiasis was 100% and the stone clearance rate was 100%.Conclusion　The key factors of ESWL for the successful treatment of urethral calculi is the appropriate treatment position and B ultrasonic positioning methods according to the location of the urethral calculi.]]> 2018/3/28 17:03:21 LU Xiong-jin, PANG Ye-xiao, LI Ruo-pan, et al. 226true ［Abstract］　Objective　To explore the efficacy of intrauterine misoprostol administration combined with oxytocin in prevention of postpartum hemorrhage after cesarean section.Methods　From May 2014 to May 2017, three hundred women undergoing cesarean section were randomly divided into two group, with 150 cases in each group. Oxytocin 10 U was injected into the uterine body after the babies were delivered in the control group, followed by intravenous infusion of oxytocin 20 U added into 500 ml glucose injection. Oxytocin 10 U was injected into the uterine body after the babies were delivered in the research group, followed by intrauterine misoprostol administration 0.4 mg. The amount of bleeding during operation, postpartum 2 hours and postpartum 24 hours, the incidence of postpartum hemorrhage, duration of third stage and adverse reactions were compared between the two groups.Results　The amount of bleeding during operation, postpartum 2 hours and postpartum 24 hours in the research group were significantly less than those in the control group(P<0.05). The incidence of postpartum hemorrhage and duration of third stage in the research group were significantly lower or shorter than those in the control group(P<0.05). There was no obvious adverse reaction in the two groups.Conclusion　Intrauterine misoprostol administration combined with oxytocin is safe and effective in prevention of postpartum hemorrhage after cesarean section.]]> 2018/3/28 17:03:21 LIU Wei-man 225true ［Abstract］　Objective　To compare transcranial Doppler(TCD) foam test and transthoracic echocardiography(TTE) in diagnosis of cryptogenic ischemic stroke(CS) in middle-aged and young patients.Methods　110 patients(aged 18 to 60 years old) with cryptogenic ischemic stroke in our hospital from August 2015 to August 2017 were selected according to the TOAST classification. The positive rate and the characteristics of general information and risk factors of stroke patients and imaging characteristics of TCD foaming test and TTE results were compared.Results　The positive rate of TCD foaming test(44.5%) was significantly higher than that of TTE(31.8%). The detection rates of the two methods were gradually increased. There was no significant difference in age between the patients with negative TCD foaming test and those with positive result（P>0.05）. High blood pressure, high blood cholesterol and smoking were the risk factors. The positive patients had no obvious stroke caused by traditional risk factors compared to those with negative result. There was no significant difference in vascular lesion distribution between the positive and negative infarction patients. The lesions in the patients with positive infarction mostly occurred in the cortex or subcortical region, while the lesions in the negative patients were prone to be in deep gray areas.Conclusion　TCD foam test is better to find the cause of cryptogenic stroke in young and middle-aged patients with patent foramen ovale. The most of positive multiple lesions are in the cortical or subcortical regions, which has an important value in the etiological diagnosis of stroke.]]> 2018/3/28 17:03:21 CHEN Wen-xin, HUANG Miao-shan, CHEN Han-jie, et al. 224true ［Abstract］　Objective　To study the relationship between the expression changes of NIP3-like protein X（NIX） in hepatic stellate cell(HSC) and the inhibitory role of ursodesoxycholic acid（UDCA） on activation of HSC.Methods　HSC-LX2 cells were divided into four groups: vehicle control group,UDCA(0.5 μM) group, TGF-β1(5 ng/ml) group, and TGF-β1(5 ng/ml)+UDCA(0.5 μM) group. The expressions of α-SMA and NIX were detected by Western blot and were compared among the different groups.Results　After HSC-LX2 cells were stimulated by TGF-β1, the expression of α-SMA in LX2 cells was 60.9% higher than that in the control group, and the expression of NIX was simultaneously elevated with an increase of 51.0%. The expression of a-SMA was inhibited 55.6% by UDCA in LX2 cells which were activated by TGF-β1, and that was positively correlated with the significantly down-regulated expression of NIX.Conclusion　UDCA reduces the expression of NIX protein in HSC, which might be one of the pharmacological mechanisms of UDCA to counteract liver fibrosis.]]> 2018/3/2 17:10:11 TENG Lin-hua, LIU Qi-lin, LIU Ze-feng, et al. 223true ［Abstract］　Objective　To study the influence on smile beauty and the relevant factors with skeletal class Ⅱ malocclusion by extraction and myofunctionaltherapy.Methods　Twenty permanent dentition patients of skeletal class Ⅱ malocclusion were selected. All the research subjects had been treated with maxillary and mandibular straight-wire technique by extraction and using additional enhancement palatal measures. The frontal smile photographs were measured with Adobe Photoshop CS7(including curvature of forward tooth, lower lip curvature, buccal corridor, smile width, smile height, smiling arc and upper lip shape), All of the measurements were analyzed by SPSS version 17.0.Results　After treatment, the curvature of upper incisor was increased; Buccal space ratio increased; Upper incisor ratio increased, and exposure of lower incisors was decreased(P<0.05). The number of ideal smile arc curvature and upward upper lip increased significantly(P<0.05). There was a negative correlation between the change of ∠U1-SN and the changes of the curvature change of upper incisor. There was a negative correlation between the change of ∠SN-MP and the change of buccal space ratio.Conclusion　The permanent dentition patients of skeletal class Ⅱ malocclusion are treated with smile myofunctional therapy, and the smile measurements are improved after treatment. The changes of smile arc are correlated with the changes of upper incisors inclination. The changes of buccal corridor are correlated with the changes of the vertical height.]]> 2018/3/2 17:10:11 LU Hui-yuan, ZHOU Yan, FANG Zhi-xin, et al. 222true ［Abstract］　Objective　To observe the changes in contour of working blades in Gracey curette after various speed controlled sharpening methods, and to provide the basis for improving the sharpening style of the instrument.Methods　274 working ends from 137 discarded Gracey curettes were selected and compared to 4 brand new curettes which were taken as the controls. Comparative observations of the changes in appearances after sharpening were performed for the toes, cutting edge angle, face and back of each instruments. The length and breadth of the cutting edge were measured and compared. Plastic stick was used to test sharpness.Results　19 working edges were broken. If observed from the faces of the rest 255 working edges, the double edged blade angle was found to be parallel in 178 instruments(69.80%); the cutting edge remained unchanged in 121(47.42%) cases. Similarly, only in 38(14.90%) working ends rounded contour of the toes were maintained whereas only in 7(2.75%) instruments the back of the cutting edges was still smooth. The average length and breadth of the working edges of these discarded curettes were also found to be decreased in measurement. 77 working edges were reported sharp in the test.Conclusion　Sharpening of Gracey curettes produces deformation in shape and size that affects the effectiveness of the instruments during performance. More attention should be given to the proper sharpening technique.]]> 2018/3/2 17:10:11 ZENG Qi-xin, WU Jia-xuan, WANG Jia-xi, et al. 221true ［Abstract］　Objective　To compare the measure results of serum HBV-LP between using time-resolved fluoroimmunoassay(TRFIA) and enzyme-linked immunosorbent assay(ELISA) in the patients with hepatitis B.Methods　A total of 150 serum specimens of hepatitis B patients were collected. 30 serum specimens of healthy people were taken as the negative controls. HBV-LP of 150 serum specimens was measured by ELISA to distinguish 70 negative from 70 positive samples. Then, these samples were measured by TRFIA. The sensitivity, specificity, consistency, detection range, precision, relativity and the stability of kits were evaluated. 12 inconsistent samples between using ELISA and TRFIA were tested by polymerase chain reaction(PCR).Results　The sensitivity of TRFIA and ELISA was 0.1 ng/ml and 2.5 ng/ml respectively. The specificity of TRFIA and ELISA was 100%. The Kappa was 0.83. The HBV DNA copies of 10 inconsistent samples were>10³. The detection range of TRFIA was between 0.625 ng/ml and 10 252 ng/ml. The positive rate of ELISA was between 5 ng/ml and 1 281.6 ng/ml. The intra-assay average CVs of TRFIA and ELISA were 6.10% and 8.98% respectively for 3 different concentrations. The inter-assay average CVs of TRFIA and ELISA were 6.91% and 10.45% respectively. The stability of the established assay kit was better than that of the commercially ELISA kit.Conclusion　TRFIA and ELISA come with a high degree of consistency in detecting HBV-LP. However, the sensitivity, precision and stability of TRFIA are better than those of ELISA.]]> 2018/3/2 17:10:11 LI Mei, LIU Jie, YE Yan, et al. 220true ［Abstract］　Objective　To explore the effects of different surgical methods on the treatment of oxidative stress and immune function in uterine fibroids, and to provide a basis for future treatment of uterine fibroids.Methods　100 patients with uterine fibroids were collected in our hospital from April 2016 to February 2017, and were divided into the LM group(laparoscopic myomectomy), the TCRM group(transcervical resection of myoma) and the AM group(abdominal myomectomy) according to the different surgical methods. The oxidative stress and immune function were compared among the three groups two months after the treatment.Results　The body monocyte chemotactic protein in oxidative stress indicators - l(MCP - 1) and activation adjustment factor(RANTES) in the LM group and the TCRM were obviously lower than those in the AM group(P<0.05). In addition, the immunoglobulin IgM, IgA and IgG in the LM group and the TCRM group were significantly higher than those in the AM group(P<0.05).Conclusion　Laparoscopic myomectomy and transcervical resection result in less oxidative stress and less impact on immune function and more suitable for removing uterine fibroids than abdominal myomectomy.]]> 2018/3/2 17:10:11 LIANG Wei-yan 219true ［Abstract］　Objective　To study the epidemiological characteristics of injuries among community residents of Nanning city in 2013.Methods　A retrospective survey was conducted in South-Taoyuan community of Nanning city through probability proportional to size cluster sampling(PPS) in 2013. The incidence, the locations, the causes, and the consequences of injuries were statistically analyzed.Results　The incidence of injuries among the community residents of Nanning city was 22.92‰. Homes, schools and streets were the major locations of the injury events whose injury percentages were 7.9‰, 6.6‰ and 3.2‰ respectively.The injured patients aged ≥60 years（36.9‰） were the climax age group of accidents, followed by those aged 15 to 19 years（32.0‰） and those aged 5 to 14 years（26.5‰）. Accidental falls were the leading cause（7.7‰）, followed by traffic injuries（6.6‰）, and being wounded by someone or something （4.8‰）. The common categories of injuries were sprains and strains（7.5‰）, fractures（2.9‰） and contusion（4.6‰）.Conclusion　The incidence of injuries among community residents in Nanning is higher than that in the well-developed districts in China.prevention and intervention measures should be taken in accordance with the different causes of the injuries.]]> 2018/3/2 17:10:11 LU Yi-jun, LU Jun-yu, PAN Chun-xi, et al. 218true ［Abstract］　Objective　To explore the relationship between symptoms, signs and different levels of Alpha-fetoprotein(AFP) in the patients with primary hepatocellular carcinoma(PHC).Methods　SPSS19.0 was used to statistically analyze the symptoms and signs of PHC patients with different levels of AFP.Results　A total of 1 030 PHC patients were included in this study. The results of the Logistic regression analysis showed that the following factors have a significant effect on AFP levels: age, abdominal pain, bloating, block type, type of liver cells and bile duct cell type. The results from the linear trend analysis indicated that the percentage of positive cirrhosis, abdominal pain, bloating, ascites, hepatomegaly, massive, HBsAg, HBeAg and Anti-HBc positive and HBsAg, Anti-HBe and Anti-HBc positive increased with elevated AFP, while the percentage of positive nodular, type of liver cells and bile duct cell type decreased with elevated AFP.Conclusion　The serum levels of AFP should be dynamically detected and the clinical symptoms and signs should be observed carefully to make the PHC patients treated more timely and effectively.]]> 2018/3/2 17:10:11 ZHOU Xiao-ping, ZHANG Ling, WU Jun-rong 217true ［Abstract］　Objective　To study the difference in transthoracic chest ultrasound sonograms between the patients suffering from interstitial lung disease(ILD) and acute exacerbation of chronic obstructive pulmonary disease(AECOPD).Methods　70 patients with ILD(the ILD group) and 30 patients with AECOPD(the AECOPD group) who were confirmed by computed tomography(CT) and clinical diagnoses underwent transthoracic lung ultrasound for assessment of pleural thickening, uneven pleural surface, subpleural low echoarea, pleural effusion, and the number of B lines in the section with the most ones were recorded.Results　Transthoracic lung ultrasound examination showed the proportion of pleural thickening more than 1.0 mm, a concave convex uneven pleura, with 3 B lines in a section and subpleural low echoarea in the ILD group were higher than that in the AECOPD group. There were significant differences between them(P<0.01). And the proportion of pleural effusion in the ILD group was lower than that in the AECOPD group, with a significant difference between the two group(P<0.05).Conclusion　The transthoracic chest ultrasound sonograms have some specific features for diagnosing ILD, which can be used to differentiate ILD from AECOPD.]]> 2018/3/2 17:10:11 HUANG Xiang-hong, HUANG Xiao-li 216true ［Abstract］　Objective　To analyze the effect of cognitive impairment on the clinical efficacy of rehabilitation therapy in patients with pseudobulbar palsy induced by brain injury.Methods　50 patients with pseudobulbar palsy induced by brain injury were treated with comprehensive swallowing rehabilitation therapy, including 25 cases of cognitive impairment who were given extra cognitive training. The swallowing disorders were assessed by Watian drinking water test, standardized swallowing assessment(SSA) and video fluoroscopic swallowing study(VFSS) before and after the therapy.Results　The swallowing improvement effect of cognitive impairment in the patients with pseudobulbar palsywas was worse than that in the patiens with only pseudobulbar palsy, which demonstrates the negative effect of cognitive impairment on pseudobulbar palsy.Conclusion　Cognitive function affects the rehabilitation effect of swallowing therapy. It is important for the patients with pseudobulbar palsy to get their cognitive function improved if they have disgnosia.]]> 2018/1/26 9:57:02 TIAN Shan, WU Jun-fa, LIU Gang, et al. 215true ［Abstract］　Objective　To observe the effect of oral comprehensive intervention on oral health status and blood glucose control in the patients with type 2 diabetes mellitus complicated with periodontal disease.Methods　146 patients with type 2 diabetes mellitus complicated with periodontal disease were divided into the experimental group(n=75) and the control group(n=71). Both groups were given oral hygiene education and effective tooth brushing. In addition, the experimental group was given supragingival scaling and(or) subgingival curettage of periodontal intervention. The periodontal indexes, subgingival plaque of porphyromonas gingivalis in content, glycosylated hemoglobin, fasting blood glucose were compared between the two groups.Results　Comparison of the periodontal index between the two groups at different stages: 3 and 6 months after the treatment, the periodontal indexes in the experimental group were declined while they were increased in the control group compared with those at the previous stage, with significant differences between the two groups(P<0.05). 12 months after the treatment, the periodontal indexes were increased in both of the two groups compared with those at 6 months(P<0.05). Comparison of the subgingival plaque Pg detection between the two groups at different stages: 3 months after the treatment, the subgingival plaque of Pg in the experimental group was decreased compared with the baseline value while it was increased in the control group, with a significant difference between the two groups(P<0.05). 6 and 12 months after the treatment, it was increased in both groups compared with the previous stage increased, without significant differences between the two groups(P>0.05). Comparison of the fasting blood glucose between the two groups at different stages: 3 months after the treatment, FBG in the experimental group decreased while it increased in the control group, without significant differences between the two groups(P>0.05). 6 months after the treatment, FBG increased in both of the two groups, without a significant difference(P>0.05); 12 months after the treatment, FBG in the experimental group decreased while it increased in the control group, without a significant difference(P>0.05). Comparison of hemoglobin between the two groups at different stages: 3 months after the treatment, HbA1c was lower than that of the baseline value, without a significant difference between the two groups(P>0.05). 6 months after the treatment, HbA1c in the experimental group decreased compared with that of the baseline value while it increased in the control group, with significant differences between the two groups(P<0.05); 12 months after the treatment, HbA1c in the experimental group increased compared with that 6 months after the treatment, while HbA1c was unchanged in the control group, without a significant difference between the two groups(P>0.05).Conclusion　Periodontal intervention can not only improve the periodontal status in the patients with type 2 diabetes mellitus complicated with periodontal disease, but also play a positive role in the control of diabetes mellitus. It is suggested that oral health examination should be carried out once every six months and the patients should cooperate with the doctors to carry out the periodontal comprehensive intervention actively.]]> 2018/1/26 9:57:02 LU Yan, ZHOU Yan, LIU Hong-yan, et al. 214true ［Abstract］　Objective　To explore the application value of diffusion weighted image in diagnosing cervical carcinoma and its correlation with pathology.Methods　Of the 88 patients with cervical cancers, 84 suffered from squamous cell carcinoma(gradeⅠ in 16 cases, gradeⅡ in 46 cases and grade Ⅲ in 22 cases), and 4 suffered from adenocarcinoma. Other 88 healthy women during non-menstrual or menopausal period were taken as the controls. The differences of ADC value were compared among cervical cancer, normal cervix, squamous cell carcinoma and adenocarcinoma, and the area under the ROC was simultaneously plotted to evaluate the diagnostic effect. The ADC values of different grades of cervical cancers and their correlation were analyzed.Results　The ADC value of cervical cancer［（0.842±0.151）10^-3mm²/s］ was lower than that of normal cervix［（1.387±0.087）10^-3mm²/s］, and the ADC value of squamous cell carcinoma［（0.835±0.149）10^-3mm²/s］ was lower than that of adenocarcinoma［（0.999±0.083）10^-3mm²/s］ whose area under the ROC was 0.991 and 0.908 respectively. The ADC values of gradeⅠ, Ⅱ and Ⅲ were ［（1.059±0.155）, （0.847±0.043） and （0.680±0.084）10^-3mm²/s］ respectively.Conclusion　DWI can provide more information than the morphology for diagnosing cervical carcinoma, and help to improve the pathological classification and grading.]]> 2018/1/26 9:57:02 WEI Jie-qin, LU Li-jian, BAI Yang, et al. 213true ［Abstract］　Objective　To investigate the effects of caffeine citrate and aminophylline therapy on neuropsychological development in preterm infants.Methods　A total of 120 preterm infants who were diagnosed with primary apnea were randomly divided into the caffeine group(n=60) and the aminophylline group(n=60). Gesell development scale(GDS) was used to assess the neuropsychological development in the preterm infants when they were followed-up regularly after discharge. The hospital course, morbidity rate and neuropsychological development were compared between the two groups at 12 months of corrected age and at 24 months.Results　The disappearance time of apnea, hospital stay, duration of drug therapy and the oxygen therapy in the caffeine group were significant shorter than those in the aminophylline group(P<0.05). The incidence rates of feeding intolerance and intracranial hemorrhage in the caffeine group were significantly lower than those in the aminophylline group(P<0.05). The developmtntal quotient(DQ) scores of gross motor, fine motor, adaptability and social communicative abilities in the caffeine group were significantly higher than those in the aminophylline group at 12 months of corrected age. However, the DQ scores of gross motor and fine motor in the caffeine group were significantly higher than those in the aminophylline group at 24 months.Conclusion　Caffeine citrate therapy is more conducive to the improvement of primary apnea and the neuropsychological development in premature infants.]]> 2018/1/26 9:57:02 HUANG Hai-yan, LU An-feng, HUANG Guo-sheng, et al. 212true ［Abstract］　Objective　To explore the changes of resonance characteristics of the nasal cavity, including nasal sinuses and nasal airway resistance(NAR)after transnasal 70°endoscopic surgery of fungus ball maxillary sinusitis.Methods　Visual analog scale, NAR and acoustic analyses were performed on 32 patients with fungus ball maxillary sinusitis before and after the surgery.Results　Of the 32 patients, 28 were cured, 2 improved and 2 relapsed.The scores of VSA scale were significantly reduced three months after the surgery(P<0.01). The effective resistances in inspiration, expiration and vertex resistance in inspiration,expiration were not significantly different before and after the surgery. There was an increase in the values of the vowel in the first formant. No significant differences were found before and after the surgery regarding fundamental frequency, the first, second and third formants of the vowel.Conclusion　The transnasal 70°endoscopic surgery can improve the symptoms of the patient with fungus ball maxillary sinusitis. The surgery has no influence on the resistance of the nasal cavity. The resonance characteristics of the nasalcavity are changed slightly after the surgery. Some patients such as voice professionals should be informed about the possible alterations in speech before the surgery.]]> 2018/1/26 9:57:02 ZHANG Shao-jie, WANG Rong, LIANG You-qiong 211true ［Abstract］　Objective　To investigate the effect of microskin grafting on matrix metalloproteinase-2(MMP-2) and MMP-9 levels in diabetic ulcer. Methods　Eighteen cases of diabetic ulcers were treated with microskin grafting. The wound granulation tissues were sampled for detection of MMP-2 and MMP-9 levels before and 7 d, 14 d after treatment using Enzyme-Linked Immunosorbent Assay. The areas of the ulcers were observed and measured. Results　The area of ulceration after microskin grafting treatment was significantly reduced at each observation time point compared with that before treatment(P<0.05). The concentrations of MMP-2 in the granulation tissues gradually decreased and reduced to the lowest level on the 14th day after treatment(P<0.05). MMP-9 decreased gradually after treatment, but in each time point there was no significant difference in MMP-9 level compared with that before treatment. There was a positive correlation between the concentration of MMP-2 in granulation tissue and the size of ulcer(r=0.385, P<0.05). Conclusion　Microskin grafting therapy can reduce the concentration of MMP-2 in the granulation tissues of diabetic ulcers, and the concentration of MMP-2 may be a relevant factor influencing the healing of diabetic ulcers.]]> 2018/12/29 16:55:29 QI Shi-ling, ZHU Ding-heng, DI Da-ke, et al. 210true ［Abstract］　Objective　To explore the operative method and effect of reoperation on postoperative recurrence of secondary hyperparathyroidism(SHPT) in the patients with uremia. Methods　The clinical data of 10 uremia patients with postoperative recurrence of SHPT were retrospectively analyzed. The clinical symptoms, the levels of serum intact parathyroid hormone(iPTH), blood calcium and blood phosphorus, and the reoperation outcomes and complications were compared before and after the surgery. Results　Parathyroidectomy of neck remnants and parathyroidectomy with autologous forearm transplantation were performed on the 10 patients. The symptoms of osteodynia and skin itch disappeared in the patients after surgery. Compared with those before surgery, the levels of iPTH, blood calcium and blood phosphorus were significantly reduced after surgery（P<0.05）. No recurrence was found after 6 months of follow-up. Conclusion　Reoperation is effective for postoperative recurrence of secondary hyperparathyroidism in the patients with uremia. Preoperative localization should be combined with various imaging positionings and all the residual parathyroid tissues should be removed to avoid repeated episodes of SHPT.]]> 2018/12/29 16:55:29 LIAO Dan, ZHENG Hou-pu, ZHANG Meng-die, et al. 209true ［Abstract］　Objective　To explore the advantages and disadvantages of anticoagulation without calparine in continuous renal replacement therapy(CRRT) for children with acute kidney injury(AKI)at high risk of bleeding. Methods　Twenty-two children with AKI at high risk of bleeding were treated with CRRT and they were randomly divided into two groups: anticoagulation without calparine group(11 case, 33 times of CRRT) and heparin-free group (11 case, 38 times of CRRT). Continuous veno-venous hemodialysis filtration(CVVHDF) mode was employed on both groups. The serum creatinine,function of the coagulation system were monitored before and after CVVHDF. The vital signs, dialyser clotting and the incidence of bleeding episodes were recorded in the patients during treatment. Results　The serum levels of creatinine decreased significantly after treatment in both groups, but the reduction rate was obviously higher in the anticoagulation without calparine group than that in the heparin-free group［(80.2±8.5)% vs (70.3±10.5)%, P<0.05］. The average duration of CVVHDF treatment was (18.2±6.6)h in the anticoagulation without calparine group and (10.5±3.5)h in the heparin-free group, with a significant difference between the two groups(P<0.05).There was no significant difference in the function of the coagulation system between pre-and post-CVVHDF in both groups(P>0.05).The vital signs were stable and no bleeding episodes were found in all the patients in the two groups. Conclusion　Anticoagulation without calparine is safe and effective for CRRT in children with acute kidney injury at high risk of bleeding.]]> 2018/12/29 16:55:29 LI Guang-bo, LI Hong-jiang, LIU Cui-hua 208true ［Abstract］　Objective　To establish the reference intervals of thyroid function for pregnant women during different periods of pregnancy in Daqing area. Methods　The data of serum free triiodothyronine(FT₃), free thyroxine(FT₄) and thyrotropin(TSH) were collected from 469 pregnant women during different periods of pregnancy, and 95% reference interval was established with median(M) and percentile(P_2.5～P_97.5 ). Results　The reference intervals: Early pregnancy group 1: FT₃ 3.98～7.49 pmol/L, FT₄ 8.04～20.32 pmol/L, TSH 0.14～3.65 mIU/L; Early pregnancy group 2: FT₃ 3.91～6.43 pmol/L, FT₄ 8.1～15.42 pmol/L, TSH 0.06～5.12 mIU/L; Mid-pregnancy: FT₃ 4.03～5.96 pmol/L, FT₄ 6.71～14.12 pmol/L, TSH 0.18～4.72 mIU/L; Late pregnancy: FT₃ 3.96～6.08 pmol/L, FT₄ 6.74～14.26 pmol/L, TSH 0.84～4.98 mIU/L. The detection rates of hypothyroidism screened by this standard and the Guidelines of Thyroid Disease Standard were 0%(0/469) and 1.1%(5/469)respectively. The detection rate of this standard was lower than that of the standard(P=0.025). The detection rates of subclinical hypothyroidism by this standard and the Guidelines of Thyroid Disease Standard were 1.7%(8/469) and 10.0%(47/ 469) respectively. The detection rate of the standard was higher than that of this standard(P=0.000). Conclusion　Establishment of the specific reference intervals of gestation can diagnose thyroid dysfunction accurately and timely during pregnancy.]]> 2018/12/29 16:55:29 WANG Li-qiu, ZHANG Feng-xia, LIU Fang-he, et al. 207true ［Abstract］　Objective　To investigate the value of superb microvascular imaging(SMI) for diagnosing pannus synoviae of rheumatoid arthritis(RA). Methods　The synovial flow signals of the supraplallar patella and the infrapatellar pouch of 38 patients with RA undergoing total knee arthroplasty were detected by power Doppler ultrasound(PDUS) and superb microvascular imaging(SMI) respectively. The signals of synovial flow were recorded and graded. Synovial pathology was also graded in 92 knees of the suprapatellar and subretinal sacs. The consistency of synovial grading was compared between PDUS and SMI, and the correlation between SMI classification and pathological grade was analyzed. Results　Among the 38 patients, 46 knees were performed total knee arthroplasty. The displaying rate of PDUS blood flow signal was 99.0% and that of SMI blood flow signal was 100.0% in the thickening synovial memberane. The consistency of PDUS and SMI synovial blood flow signal classifications was high(Kappa=0.630, P<0.05). There was a positive correlation between SMI classification and synovial pathological grade(r=0.736, P<0.05). Conclusion　Compared with PDUS, SMI has a high detection rate of synovial flow signals.There is a high correlation between SMI classification and pathological grade. SMI technique has a good detection effect on the synovial membrane of RA patients.]]> 2018/12/29 16:55:29 LIU Huan-yan, DU Jian-wen, WANG Hong, et al. 206true ［Abstract］　Objective　To evaluate the accuracy of Lenstar 900 optical biometry for measurement of axial length(AL) and central corneal thickness(CCT) in myopia. Methods　Forty-eight myopic patients (96 eyes) with spherical equivalent(SE) varied from -1.00～-6.75 D, aged 18～45(26.38±6.03)years, were recruited from the Visual Science and Optometry center of Guangxi. AL was measured by Lenstar 900 and A-scan, and CCT was measured by Lenstar 900, A-scan and Pentacam. The paired t-test was used to compare the differences of the measured results of different instruments. Pearson correlation analysis and Bland-Altman analysis were used to evaluate the correlation and the consistency of the measured results of the different methods. Results　The values of AL measured by Lenstar 900 and A-scan were (25.21±1.18) and (25.09±1.17)mm, respectively, with a significant difference between the two measured methods(P<0.01) and a significant correlation between them (r=0.982, P<0.01), and the 95% limit of agreement(LOA) was (-0.31～0.10)mm. The values of CCT measured by Lenstar 900, A-scan and Pentacam were (532.55±24.09)μm, (530.03±23.39)μm and (537.64±23.75)μm respectively. There was significant difference among the three methods(all P<0.01). Pearson correlation analysis showed that the value of AL measured by Lenstar 900 was positively correlated with the results of A-scan(r=0.985, P<0.01), and the results of Pentacam(r=0.956, P<0.01). The values of 95% LOA of CCT were (-10.6～6.0)μm for A-scan versus Lenstar 900, (-14.7～8.4)μm for Lenstar 900 versus Pentacam, and (-14.8～5.2)μm for A-scan versus Pentacam, respectively. Conclusion　AL measured by Lenstar 900 is significantly correlated with that measured by ultrasound A-scan. Similarly, CCT measured by Lenstar 900 shows significant correlation with that measured by ultrasound A-scan or Pentacam. CCT measured by Lenstar 900 shows satisfactory consistency with that measured by the gold standard ultrasound A-scan. Lenstar 900 can be used as one of the non-contact measurement tools for AL and CCT in myopia.]]> 2018/12/29 16:55:29 WANG Ying, XIAO Xin, HUANG Hui-Yao, et al. 205true ［Abstract］　Objective　To explore the prevalence of piperacillin tazobactam sodium antibodies and the characteristics of these drug-induced immune hemolytic reaction. Methods　The blood samples were collected from the patients treated with piperacillin tazobactam sodium and the direct antiglobulin test(DAT) of the red blood cells was done using the micro gel column technique. Detection of the drug antibodies was performed on the patients with positive DAT. Then the laboratory and clinical data of the patients with positive drug antibodies were collected to analyze the immune hemolysis reaction induced by the drug antibodies of piperacillin tazobactam sodium. Results　In the 125 patients who were included in this study, DAT was positive in 74 cases(59.2%) among whom 34 cases(27.2%) had piperacillin-tazobactam sodium drug antibodies. Of the patients with drug antibodies, 4 cases occurred extravascular hemolysis with slight symptoms. Conclusion　The patients treated with piperacillin tazobactam sodium may produce the related drug antibodies causing drug-induced immune hemolytic reaction.]]> 2018/12/29 16:55:29 YANG Zi-ji, MO Zhu-ning, JIAO Wei, et al. 204true ［Abstract］　Objective　To observe the effects of chronic intermittent hypoxia on pancreatic β-cell function in rats and to investigate the protective effect of glucagon like peptide-1(GLP-1) analogues liraglutide on the pancreatic β cells. Methods　36 male SD rats were randomly divided into normal control group(n=12) and intermittent hypoxia group(n=24). After 8 weeks′ intermittent hypoxia, the primary islet β cells were extracted from all the subjects in both groups. The primary β cells of the normal control group were divided into normal control subgroup and high glucose(30 mmol/L) stimulation subgroup while the intermittent hypoxia group were divided into model subgroup and liraglutide(10, 50, 100 nmol/L) treatment subgroups. The levels of 8-iso-prostaglandin(8-ISO) and glutathione peroxidase(GSH-Px) in the cell culture supernatants were detected by enzyme linked immunosorbent assay(ELISA). The apoptosis rate of islet β cells in each group was detected by Flow Cytometry and the expression of caspase-3(an apoptosis-related protein) was detected by Western blot. Results　The levels of 8-ISO were significantly higher while the levels of GSH-Px lower in the model group and the high glucose stimulation group than those in the normal control group（P<0.05）. Meanwhile, the apoptosis rates and the expressions of caspase-3 protein of the islet β cells in the model group and the high glucose stimulation group were significantly higher than those in the normal control group（P<0.05）, but those were higher in the high glucose stimulation group than in the model group. Compared with that in the model group, the 8-ISO level was significantly lower while GSH-Px level was significantly higher in the liraglutide treatment groups（P<0.05）. The expressions of Caspase-3 protein were significantly lower in the liraglutide treatment groups than those in the model group, and those in the high concentration group were more significant than those in the middle concentration group or the low concentration group. Conclusion　　Oxidative stress caused by intermittent hypoxia may be an important mechanism of the islet function damage in obstructive sleep apnea syndrome(OSAS). Regulation of oxidation production by GLP-1 analogues liraglutide may reduce the apoptosis of islet β cells and protect the pancreatic β-cell function.]]> 2018/11/22 14:41:33 ZHOU Yan, XUE Xin-xin, YU Jian, et al. 203true ［Abstract］　Objective　To compare the expressions of E-cadherin in different oral carcinogenesis stages and lymph node metastasis between human and mouse models. Methods　Immunohistochemical staining was used to detect the expressions of E-cadherin in normal oral mucosae, oral dysplasia, oral squamous cell carcinomas(OSCC) and lymph node metastases in human beings(n=24) and mice(n=12), respectively. Results　The expression rates of E-cadherin in normal oral mucosa, dysplasia, OSCC and lymph node metastasis were 100.0%, 87.5%, 0.0% and 79.2% in human beings and 100.0%, 83.3%, 0.0% and 100.0% in mice. Conclusion　　The expressions of E-cadherin are similar in the same pathological stages of human beings and mice respectively. The expression rates of E-cadherin are decreased gradually in both human beings and mice with the increased degree of pathological malignancy, but are increased in lymph node metastases. These results indicate that E-cadherin may play an important role in the occurrence and development of OSCC and submandibular lymphatic node metastase.]]> 2018/11/22 14:41:33 CHENG Li, LI Jing, GUO Meng-zhu, et al. 202true ［Abstract］　Objective　To evaluate the association of early cleavage(EC) with in vitro fertilization(IVF) embryo quality and to investigate the predictive value of EC as an indicator of subsequent embryonic development potential in vitro. Methods　A total of 3 646 individually cultured 2PN embryos from 619 IVF treatment cycles were included, and the early cleavage status and day 3 embryo scoring were assessed. The blastocyst culture of day 3 optimal embryos(n=682) and the non-optimal embryos(n=2 180) were carried out and the embryo quality evaluations were performed on day 5 and day 6 respectively. According to the results of EC observations, the embryos were divided into three groups: EC group, pronuclear breakdown(PNBD) group and non-pronuclear breakdown(NPNBD) group. Results　The mean number of blastomeres per embryo on day 3 of the EC group was significantly larger than that of PNBD group or NPNBD group（P<0.05）, and the least number was in the NPNBD group. There was no significant difference in the high-quality embryonic rate on day 3 between the EC group and the PNBD group（P>0.05）, but the high-quality embryonic rates of both groups were significantly higher than the high-quality embryonic rates of the NPNBD group（P<0.05）. The rates of frozen embryo and available embryo on day 3 in the EC group were significantly higher than those in the PNBD group and the NPNBD group（P<0.05）, and the lowest rates were in the NPNBD group. For the optimal embryonic blastocyst culture on day 3 or on day 5, the frozen blastocyst ratio of the PNBD group was significantly higher than that of the NPNBD group（P<0.05）, but on day 5, the differences in the blastocyst formation rate and the high quality blastocyst rate were not significant among the three groups（P>0.05）.The differences in total blastocyst formation rate, the total quality blastocyst rate and the total frozen blastocyst rate were not statistically significant among the three groups（P>0.05）. For the non-optimal embryonic blastocyst culture on day 3, the blastocyst formation rate, the high quality blastocyst rate, the frozen blastocyst rate, the total blastocyst formation rate, the total high quality blastocyst rate and the total frozen blastocyst rate on day 5 of the EC group were significantly higher than those of the PNBD group and the NPNBD group. Conclusion　　EC embryos have a relatively fast embryonic development, and they are more likely to develop into a high-quality cleavage embryo on day 3. EC can be used as a predictor of embryo quality on day 3. For in vitro blastocyst development, there is no significant difference between the good-quality EC embryos and the non-EC embryos on day 3. For high quality cleavage embryos, EC may not be sufficient to be an additional predictor of the development potential of blastocyst in vitro.]]> 2018/11/22 14:41:33 MAO Xian-bao, XUE Lin-tao, TAN Wei-hong, et al. 201true ［Abstract］　Objective　To investigate the guiding significance of procalcitonin(PCT) in diagnosis of potential serious infection and risk stratification in patients with neutropenia and fever. Methods　Based on prospective cohort study design, 90 febrile neutropenia patients who were treated in Tumor Hospital Affiliated to Medical College of Shantou University from July 2016 to October 2017 were divided into high-risk group and low-risk group, with 45 cases in each group according to the Chinese Guidelines for the Clinical Application of Antibacterial Drugs for Agranulocytosis with Fever（the 2010 version）. The levels of serum PCT were detected and compared between the two groups. Results　The level of serum PCT in the high-risk group was significantly higher than that in the low-risk group［7.06(0.40～100.00)ng/ml vs 1.16(0.08～5.25)ng/ml, P=0.020)］. A PCT cut-off value>1.32 ng/ml provided a sensitivity of 71.8% and a specificity of 77.5% for diagnosis of febrile neutropenia in the patients in the high-risk group［AUC=0.778(95%CI: 0.676～0.880, P<0.001)］. Conclusion　　Detection of serum PCT can be an effective auxiliary index for risk assessment of infection in patients with cancer complicated with febrile neutropenia.]]> 2018/11/22 14:41:33 ZHENG Hong-bin, LIN Rui-yun 200true ［Abstract］　Objective　To explore the expression of Versican in endometrial carcinoma and its clinicopathological correlation. Methods　The tissues of 20 patients with normal endometrial endometria, 40 patients with atypical endometrial hyperplasia and 50 patients with endometrial carcinomas were collected. Immunohistochemistrical method(Envision) was used to detect their Versican protein expressions. Results　The postive rates of Versican protein in the endometrial carcinomas, atypical endometrial hyperplasia and normal endometrial tissues were 78.0%, 57.5% and 5.0%, respectively. The expressions of Versican protein in the endometrial carcinoma and atypical endometrial hyperplasia tissues were significantly higher than those in the normal endometrial tissues（P<0.05）. In the endometrial carcinomas, the expression of Versican was associated with tumor grade, invasion depth and lymph node metastasis （P<0.05）. Conclusion　　High expression of Versican may be associated with tumor occurrence and development of endometrial carcinoma.]]> 2018/11/22 14:41:33 LI Jing-ling, WEI Hai-ming, ZHAO Ren-feng, et al. 199true ［Abstract］　Objective　To explore the value of magnetic resonance imaging(MRI) in diagnosis of cervical caicinoma parametrial infiltration. Methods　69 cervical carcinoma patients who went to see the doctors at the first time in the Second Affiliated Hospital of Luohe Medical College from March 16 2011 to November 20 2015 were collected as the research objects and their clinical, imaging and pathological data were analyzed. Results　The sensitivity of MRI in diagnosis of parametrial infiltration of cervical cancer was 86%, the specificity was 92%, the accuracy was 91%, Youden index was 78%. For the pelvic triad, the sensitivity was 11%, specificity was 100%, accuracy was 88%, Youden index was 11%. For the surgical specimens, the sensitivity was 88%, specificity was 95%, accuracy was 94%, Youden index was 83%. Conclusion　　MRI is the noninvasive method and has some unique advantages in diagnosis of cervical caicinoma parametrial infiltration.]]> 2018/11/22 14:41:33 LI Guang-hui, ZHOU Shan 198true ［Abstract］　Objective　To explore the clinical features of pertussis-like syndrome in newborns. Methods　The clinical data of 16 newborns with neonatal pertussis-like syndrome admitted to our department during January 2014 and December 2016 were analyzed. Results　A tatal of 16 samples were in clueled in the study. There were 9 male infants, 7 female infants and 2 premature infants. The onset time was 12 to 27 days. All of the cases had paroxysmal cough. 10 cases had a history of exposure to cough. 3 cases had fever. 7 cases had crow-like echo. 5 cases showed early nasal congestion and runny nose. The peripheral white blood cell count was increased in 13 cases and lymphocytes increased in 10 cases. 10 cases had positive deep sputum culture. 5 cases had respiratory syncytial virus infections. Some patients were treated with gamma globulin on the basis of erythromycin treatment. Convulsion and cough were improved in all the patients and they were discharged without any deaths. Conclusion　　Neonatal pertussis-like syndrome is similar to neonatal pertussis in the clinical symptoms. The newborns who have paroxysmal spasmodic cough, increasing peripheral blood white blood cells, and the rise of lymphocytes should have the Bordetella Pertussis PCR test to confirm the neonatal pertussis-like syndrome, and are treated timely.]]> 2018/11/22 14:41:33 WANG Hua, LU Yan, ZHU Wen, et al. 197true ［Abstract］　Objective　To investigate the current situation of HIV/AIDS patients′ medical coping styles and the influencing factors of the patients′ coping styles. Methods　A cross-sectional study was carried out in 221 people living with HIV/AIDS(PLWHA) using Medical Coping Modes Questionnaire(MCMQ) scoring and the results of the scoring method were compared with those of Chinese normal model. Results　The results showed that the scores of confrontation in the PLWHA were lower than those in the normal model, while the scores of avoidance and acceptance-resignation were higher than the normal model. Gender and educational level had statistically significant influences on MCMQ scores in the PLWHA. On the avoidance factor, the male patients scored higher than the female patients(P=0.038). On the acceptance-resignation factor, there were significant differences in different education levels of the PLWHA. There were significant differences in MCMQ scores between the illiterate patients and the patients with primary education level（P=0.05）. There were significant differences in MCMQ scores between the illiterate patients and the patients with high school or technical secondary school education levels（P=0.014）. Conclusion　　There are significant differences in the medical coping styles between the PLWHA and the Chinese normal model. The PLWHA tend to have avoidance and acceptance-resignation coping styles rather than facing coping style. The economic status, gender, education level have impacts on MCMQ scores in the PLWHA.]]> 2018/11/22 14:41:33 HE Mei, ZHANG Ling, YAN Li-fu, et al. 196true ［Abstract］　Objective　To investigate the optimal conditions for the differentiation of peripheral blood hematopoietic stem cells into hepatic stem cell-like cells in vitro. Methods　The patients with autologous peripheral blood stem cell transplantation in our hospital were selected as the research objects. The patients′ peripheral blood mononuclear cells after the mobilization with rhG-CSF(recombinant human granulocyte-colony stimulating factor)+chemotherapeutic drugs were collected by blood cell separator, and the levels of CD34⁺ were detected by flow cytometry, and the CD34⁺ cells were purified. The CD34⁺ cells were divided into 5 groups: control group, HGF（hepatic growth factor） group, FGF4 （fibroblast growth factor） group, FGF4+HGF group and HGF+FGF4+MCSF（recombinant human macrophage colony stimulating factor） group. The morphological changes of cells were observed dynamically. The expressions of Oct4, ALB, AFP, c-Kit, CK19 and CD34 were detected with immunofluorescence to identify the characters of the differentiated cells after the cells were cultured for 15 days. Results　In the HGF+FGF4+MCSF group: a large number of round cells, adherent growth, nucleolus obvious, large proportion of nucleus to cytoplasm, part of the cell bead-like growth. In the FGF4+HGF group: a small number of round cells, others deformed significantly. In the control group, HGF group and FGF4 group: except for individual round cells, most cells died. In the HGF+FGF4+MCSF group: positive expressions in Oct4, c-Kit, CK19 and CD34, weak positive expression in AFP and negative expression in ALB. In the FGF4+HGF group: positive expressions in AFP and ALB, weak positive expressions in CK19 and CD34, and negative expressions in Oct4 and c-Kit. In the control group, HGF group and FGF4 group: all of the above indicators were not expressed. Conclusion　The combination of HGF, FGF4 and MCSF shows obvious advantages in inducing peripheral blood stem cells to differentiate into hepatic stem cell-like cells.]]> 2018/10/26 9:09:11 HAN Ya-min, YIN Wu, MO Ming-zheng, et al. 195true ［Abstract］　Objective　To evaluate the diagnostic value of bronchoalveolar lavage fluid(BALF) tumor marker detection in differentiating the nature of peripheral typical isolated pulmonary nodules. Methods　156 patients were included in this study who were pathologically diagnosed with solitary pulmonary nodules in our hospital from January 2015 to January 2016,including 126 cases of lung cancer(123 cases of adenocarcinoma, 2 cases of squamous carcinoma and 1 case of small cell lung cancer), 30 cases of benign lesions(7 cases of inflammatory pseudotumor, 12 cases of hamartoma, 8 cases of hemangioma and 3 cases of inflammatory granuloma). The contents of carcino-embryonic antigen(CEA), cytokeratin 19 fragment(CYFRA21-1), neurone specific enolase(NSE) and squamous cell carcinoma antigen(SCCA) in the BALF were detected and compared between the two groups. Results　The contents of CEA and CYFRA21-1 in the lung cancer group were significantly higher than those in the benign group(P<0.05).There were no significant differences in the contents of NSE and SCCA between the two groups（P>0.05). Conclusion　CEA and CYFRA21-1 in BALF have high value in diagnosing peripheral solitary pulmonary nodules, while NSE and SCCA are of limited value.]]> 2018/10/26 9:09:11 FANG Jie, WANG Tai-yong, BAI Dong-ming, et al. 194true ［Abstract］　Objective　To study primary aldosteronism(PA) aorta remodeling and its influencing factors. Methods　The blood biochemistry indexes were detected and the inner diameter of the aorta were measured in 30 patients with PA and in 30 patients with essential hypertension(EHT), respectively, and their results were compared. Results　(1)The diameters of aortic annulus, sinuses, root and ascending aorta in the PA patients were significantly wider than those in the EHT patients(P<0.05); (2)Univariate analysis showed that the PA patients′ inner diameters［aortic annulus, sinuses, root and ascending portion and standing, supine ARR(aldosterone and renin activity ratio), stand, supine PAC(plasma aldosterone concentration)］ were positively correlated; (3)Multiple stepwise linear regression analysis showed that the PA patients had: ①Aortic annulus inner diameter main linear relationship with orthostatic ARR and orthostatic PAC; ②Sinuses inner diameter main linear relationship with orthostatic ARR and supine PAC; ③Root inner diameter main linear relationship with orthostatic ARR and supine PAC; ④Ascending aorta inner diameter main linear relationship with orthostatic ARR, supineARR and orthostatic PAC. Conclusion　Aortic diameter expansion remodeling exists in PA patients. The factors influencing the aorta expansive remodeling in the parts of the aorta are various, but the orthostatic ARR is the common factor, suggesting that it may be the key to reverse the PA patients with aortic distensibility remodeling.]]> 2018/10/26 9:09:11 QIAN Jing, HUANG Rong-jie, SHI Ji-ying 193true ［Abstract］　Objective　To investigate the therapeutic effect of Jiangzhi Tongluo capsules combined with statins on hyperlipidemia and to provide more reliable evidence for clinical prevention and treatment. Methods　Databases such as Chinese Journal Full-text Database(CNKI), Wanfang Database, VIP, PubMed, Embase and Cochrane Library were searched for relevant studies published up to December 2017. Review Manager 5.3 software was used for Meta-analysis. Results　Finally, 5 articles were included in the Meta-analysis, with a total of 552 cases. Meta-analysis showed that, compared with statins alone, Jiangzhi Tongluo capsules combined with statins significantly reduced the serum total cholesterol ［SMD=-0.42，95%CI(-0.70, 0.13), P=0.004］, serum triglyceride content ［SMD=-0.40, 95%CI(-0.57, 0.23),P<0.00001］ and increased high density lipoprotein cholesterol ［SMD=0.43, 95%CI(0.14, 0.72), P=0.004］, while not significantly reduce low density lipoprotein cholesterol ［SMD=-0.80, 95%CI(-1.66, 0.07), P=0.07］. Conclusion　Compared with statins alone, Jiangzhi Tongluo capsules combined with statins can significantly reduce serum total cholesterol, triglyceride content and increase high density lipoprotein cholesterol. This conclusion still needs to be verified by further studies.]]> 2018/10/26 9:09:11 LI Yuan-xiao, TANG You-cai, LI Xuan-xuan, et al. 192true ［Abstract］　Objective　To investigate the clinical value of endoscopic mucosal resection for colorectal advanced adenoma in accurate screening of colorectal cancer. Methods　A total of 52 patients who were diagnosed with colorectal advanced adenoma by colonoscopy, and demanded endoscopic resection in the Endoscopy Room of Guangzhou Integrated Traditional Chinese and Western Medicine Hospital from January 2016 to May 2017 were collected. The lesion size, morphology and operation methods of the gland, complications and postoperative pathological types were investigated. Results　The lesions were resected by EMR in 47 cases, and by EPMR(endoscopy piecemeal mucosal resection) in 5 cases. 5 cases developed intraoperative bleeding, less than 20 ml. No cases occurred delayed bleeding and perforation. The lesions were completely resected with an en bloc resection rate of 90.4%（47/52）, and a histological curative resection rate of 88.5%（46/52）. The pathological findings showed that the lesions were tubular adenoma or tubular villous adenoma with low-grade dysplasia(LGD) in 38 cases（73.1%）, tubular adenoma or tubular villous adenoma with high-grade dysplasia(HGD) in 12 cases（23.1%） and submucosal carcinoma in 2 cases(3.8%) which was resected by surgery and both lateral and basal margins of the specimens were free of tumor cells and without surrounding lymph node metastasis. 50 patients were followed up for 3 to 15 months and the median follow-up time was 9 months. No residual tumors and recurrence were found during the period of follow-up. Conclusion　Endoscopic mucosal resection is safe and effective for colorectal advanced adenoma, and plays a key role in the accurate screening of colorectal cancer.]]> 2018/10/26 9:09:11 LI Qing-feng, LIU Zhao-zhou, QIU Wen-dan, et al. 191true ［Abstract］　Objective　To explore the impact of panhysterectomy on pelvic floor function. Methods　400 patients who were performed panhysterectomy due to benign diseases in our hospital from January 2014 to June 2017 were collected. The routine gynecological examinations, assessment of pelvic floor function and quality of sexual life questionnaire were conducted before and 3 months after operation. Results　Compared with those before operation, the strength of pelvic floor muscles of type Ⅰ and type Ⅱ, vaginal pressure and quality of sexual life questionnaire scores were significantly reduced after operation（P<0.05）. The degree of fatigue of type Ⅰ and type Ⅱ muscles, the average number of incontinence episodes weekly, the vaginal front and back wall protruding and stress urinary incontinence were significantly increased after operation（P<0.05）. Conclusion　Panhysterectomy has a significant impact on pelvic floor function. It is necessary to conduct gynecological examination and assess the pelvic floor function 3 months after operation to perform early pelvic floor rehabilitation on the patients and improve their pelvic floor function.]]> 2018/10/26 9:09:11 ZHAN Feng-li, ZHAO Ren-feng, WU Yue-lian 190true ［Abstract］　Objective　To analyse the feeding modes and nutritional status in infants aged 6 months to 3 years in Bao′an District of Shenzhen City. Methods　100 infants and young children aged aged 6 months to 3 years who received physical examination in Health Service Center of Liutang Community, Baoan District Central Hospital in Shenzhen City from January 2012 to December 2016 were selected as the research subjects, and were divided into breastfeeding group and mixed feeding group according to different feeding methods. The indexes of LAZ, WAZ and Kaup were measured. Results　With the age increased, the percentage of breastfeeding was gradually decreased, while the percentage of mixed feeding was gradually increased. The indexes of LAZ and WAZ in the breast-fed infants at different ages were higher than those in the mixed-milk-fed infants, but there were no significant differences between the two groups(P>0.05). The number of normal nutritional status of the breastfeeding infants was 42(91.30%), which was significantly higher than that of the mixed-milk-fed infants(74.74%)(P<0.05). Conclusion　Breastfeeding makes the infants to achieve an ideal nutritional status, and to promote the balance of infant nutrition.]]> 2018/10/26 9:09:11 YI Shao-ya, AI Wen-juan, XIE Ming-hua, et al. 189true ［Abstract］　Objective　To explore the clinical value of ultrasound-guided key-hole approach for microsurgical treatment of basal ganglia cerebral hemorrhage. Methods　We retrospectively analyzed the clinical data of 111 patients with hypertensive basal ganglia hemorrhage in our hospital between January 2016 and December 2017. The patients were divided into study group and control group. The study group(n=62) underwent ultrasound-guided key-hole approach for microsurgical treatment and the control group(n=49) received traditional microsurgical treatment. The clinical efficacy was compared between the two groups. Results　The operation time of the study group was significantly shorter than that of the control group(P<0.01). The intraoperative blood loss of the study group was significantly less than that of the control group(P<0.01). The postoperative hematoma clearance rate of the study group was significantly lower than that of the control group(P<0.01). The ADL scores of the study group were better than those of the control group after a follow-up of 3 months(P<0.01). Conclusion　Ultrasound-guided key-hole approach can confirm the location of hematoma accurately and protect the peripheral normal tissues and significantly improve the prognosis for the patients with basal ganglia cerebral hemorrhage.]]> 2018/10/26 9:09:11 QIN Si-jie, LIU Gui-biao, LUO Jian-she, et al. 188true ［Abstract］　Objective　To investigate the prevalence of renal impairment and its influencing factors in HIV(human immunodeficiency virus)/HBV(hepatitis B virus)-coinfected patients after initiating highly active anti-retroviral treatment(HAART). Methods　Three thousand three hundred and ninety-five HIV/HBV-coinfected patients who had completed a 12-month initiated HAART from Guangxi from January 2010 to June 2015 were enrolled in this study. Logistic regression model was conducted to analyze the factors leading to renal impairment at the 12th month after the initiation of HAART. Results　The incidence of renal impairment 12 months after initiation of antiviral therapy was 14.76%. The first-line treatment regimen containing lopinavir(AOR= 4.19, 95%CI=2.76～6.38) and the WHO stage of the initiation of treatment being 3/4 stages(AOR=1.86, 95%CI=1.49～2.33) were the risk factors of renal impairment at the 12th month after treatment. Compared with those who started HAART at the level of CD4 <200/μL, the patients who started HAART at the level of 200～349/μL (AOR=0.66, 95%CI=0.50～0.85), 350～499/μL (AOR=0.54, 95%CI=0.34～0.84) or ≥500/μL(AOR=0.36, 95%CI=0.18～0.74) had lower risk of renal impairment after 12 months of HAART. Conclusion　For HIV/HBV-coinfected patients, initiation of antiviral therapy as early as possible and selection of first-line treatment with tenofovir disoproxil fumarate(TDF) can effectively reduce the risk of renal impairment at the 12th month of treatment.]]> 2019/9/30 8:42:00 ZHU Jin-hui, YANG Wen-min, SHEN Zhi-yong, et al. 187true ［Abstract］　Objective　To compare the curative effects of minimally invasive laparoscopy and laparotomy on treatment of right hemicolon carcinoma in elderly patients and the effects of the two operation methods on postoperative inflammatory status and immune function. Methods　The clinical data of 75 patients with right hemicolon carcinoma who were admitted to our hospital from January 2017 to June 2018 were analyzed retrospectively. According to different operation methods, the patients enrolled were assigned to laparoscopy group(n=36) and laparotomy group(n=39). The surgical effects, postoperative inflammatory status and the changes of T cell subsets were compared between the two groups. Results　The operation time and the length of incision in the laparoscopy group were significantly shorter than those in the laparotomy group（P<0.05）, and the amount of blood loss in the laparoscopy group was significantly less than that in the laparotomy group（P<0.05）. The levels of serum C-reaction protein(CRP) and interleukin-6(IL-6) in the laparoscopy group were significantly lower than those in the laparotomy group（P<0.05）. Compared with those before operation, the levels of CD3⁺, CD4⁺ and CD4⁺/CD8⁺ were significantly decreased in both of the two groups one day after operation, but the decreasing degrees of them in the laparoscopy group were significantly less than those in the laparotomy group(P<0.05). Compared with those before operation, the levels of CD8⁺ were significantly increased in both of the two groups one day after operation, but the increasing degrees of them in the laparoscopy group were significantly less than those in the laparotomy group(P<0.05). Conclusion　Laparoscopic surgery has a better short-term efficacy in treatment of right hemicolon carcinoma and has a mild postoperative inflammatory response to the patients and low impact on the immune function, which is worthy of clinical application.]]> 2019/9/30 8:42:00 ZHU Zhou, HUANG Shun-rong, QIN Jian, et al. 186true ［Abstract］　Objective　To investigate the expression levels of transcriptional regulation of protocadherin 8(PCDH8) gene in thyroid papillary carcinoma, thyroid follicular adenoma, nodular goiter and normal thyroid tissues and to analyze the relationship between the PCDH8 gene expression and the clinicopathological feature in the patients with thyroid papillary carcinoma. Methods　One hundred and eighteen thyroid tissue specimens were selected in our hospital from 2013 to 2015 and were divided into papillary thyroid carcinoma group(n=36), follicular thyroid adenoma group(n=30), nodular goiter group(n=31) and normal thyroid tissue group(n=21) according to the pathological results. The expression of PCDH8 gene in each group was detected by fluorescence quantitative polymerase chain reaction assay(FQ-PCR). The relationship between the PCDH8 gene expression and the clinicopathological feature in the patients with thyroid papillary carcinoma was analyzed. Results　The results of FQ-PCR showed that the negative expression rate of PCDH8 gene in the normal thyroid group(28.57%) was lower than that in the papillary thyroid cancer group(63.89%) and the thyroid follicle group(60.00%)(P<0.05), but there was no significant difference in the negative expression rate between the normal thyroid group and the nodular goiter group(54.84%)(P>0.05). The transcriptional expression of PCDH8 gene was significantly correlated with the size of the tumors(P<0.05). Conclusion　The expression of PCDH8 gene in papillary thyroid cancer is down-regulated, which is associated with the size of the tumor. PCDH8 gene may be a potential biomarker for the diagnosis and treatment of papillary thyroid carcinoma.]]> 2019/9/30 8:42:00 ZHU Ming-zhang, LIANG Wei-xin, LAI Yong-qiang, et al. 185true ［Abstract］　Objective　To investigate the effects of salvianolic acid on serum inflammatory factors and vascular endothelial function in young and middle-aged patients with cerebral infarction, and to provide reference for the treatment of cerebral infarction. Methods　One hundred and fifty young and middle-aged patients with cerebral infarction were prospectively enrolled. According to the method of random number table, the patients were divided into observation group and control group, with 75 cases in each group. The patients in the control group were given secondary prevention and treatment of cerebral infarction, while the patients in the observation group received the same treatment as the control group plus salvianolic acid. The levels of serum inflammatory factors including interleukin-6(IL-6), interleukin-17(IL-17) and hypersensitive C-reactive protein(hs-CRP) were recorded in the two groups before and after treatment. The changes of plasma endothelin-1(ET-1), nitric oxide(NO) and vascular endothelial growth factor(VEGF) levels before and after treatment were used to evaluate the vascular endothelial function. Neurological function was assessed with the National Institute of Health Stroke Scale(NIHSS) before and after treatment. After a follow-up of 6 months, the incidence rates of adverse events and adverse drug reactions were compared between the two groups. Results　There were no significant differences in the levels of IL-6, IL-17, hs-CRP, ET-1, NO, VEGF and NIHSS between the two groups before treatment(P>0.05). The levels of IL-6, IL-17, hs-CRP, ET-1 and NIHSS after treatment were significantly lower than those before treatment in the two groups(P<0.05), and the levels of NO and VEGF were significantly higher than those before treatment(P<0.05). The levels of IL-6, IL-17, hs-CRP, ET-1 and NIHSS in the observation group were significantly lower than those in the control group(P<0.05). The levels of NO and VEGF in the observation group were significantly higher than those in the control group(P<0.05). There were no significant differences in the incidence rates of adverse events and adverse drug reactions between the two groups(P>0.05). Conclusion　Salvianolic acid can significantly reduce the levels of pro-inflammatory factors in young and middle-aged patients with cerebral infarction, and improve the vascular endothelial function. Salvianolic acid is safe for the patients.]]> 2019/9/30 8:42:00 ZHANG Xiao-han, XU Bing-xin, ZHAO Yan, et al. 184true ［Abstract］　Objective　To explore the feasibility of Vacuum Assisted Sampling System for minimally invasive treatment of breast abscess during lactation. Methods　The clinical data of 141 cases of breast abscess treated with minimally invasive puncture, abscess aspiration and continuous negative pressure drainage using Vacuum Assisted Sampling System from December 2013 to June 2017 were retrospectively analyzed. Results　A total of 141 patients with breast abscesses during lactation were selected into the study, 3 cases of which were bilateral breast abscesses, a total of 144 lesions. Twenty-three cases were converted to Vacuum Assisted Sampling System with minimally invasive puncture and aspiration combined with continuous negative pressure drainage due to difficulty of aspiration by thick needle puncture. All the 144 abscesses were successfully punctured and catheterized at one time, and the success rate of the operation was 100%. The amount of abscess extracted was (45.7±13.2)ml. The mean time of negative pressure drainage was (7.6±2.1)days, and the mean time of hospital stay was (8.8±3.1)days. The cure rate was 100%. No recurrence was found after a follow-up of one month. Conclusion　The Vacuum Assisted Sampling System with minimally invasive puncture and abscess aspiration combined with continuous negative pressure drainage can be used for breast abscess during lactation with high success rate, complete drainage and quick recovery. It can save medical resources, and is easy to be accepted by the patients. This method is worthy of clinical use and promotion.]]> 2019/9/30 8:42:00 XIAO De-quan, LIU Qing-yi, XIAO Ze-wen, et al. 183true ［Abstract］　Objective　To observe the clinical efficacy of Long′s Bonesetting combined with blade needle in treatment of cervical vertigo, to evaluate the effectiveness and safety of the treatment, and to provide reliable basis for clinical application of the treatment. Methods　Fifty-six cases of cervical vertigo were randomly divided into treatment group and control group by random number table method, with 28 cases in each group.The treatment group was treated with Long′s Bonesetting combined with blade needle, and the control group received blade needle alone, with two courses of treatment in the two groups. The symptoms of cervical vertigo and the scores of Function Assessment Scales were compared between the two groups. Results　Compared with those before treatment, the scores of symptoms of cervical vertigo and Function Assessment Scale were significantly elevated in both of the two groups after treatment(P<0.05). The treatment group had better effect of the symptom improvement and higher scores of Function Assessment Scale than the control group（P<0.05）. The curative effect of the treatment group was significantly better than that of the control group(P<0.05). Conclusion　Long′s Bonesetting combined with blade needle is safe and effective in treatment of cervical vertigo caused by subluxation of atlantoaxial joint and can release the spasm of the craniovertebral muscle groups and ligaments to relieve the oppression and excitement of the nerves and vessels leading to cervical vertigo.]]> 2019/9/30 8:42:00 LIU Chao-feng, LYU Ya-nan, DENG Xiang-qiang, et al. 182true ［Abstract］　Objective　To evaluate the curative effect of compound lactobacillus combined with quadruple therapy on eradication of helicobacter pylori(Hp). Methods　Eighty-six patients with anterior gastric sinus ulcers positive Hp infection from April 2017 to June 2018 were selected as the research subjects. The patients were divided into control group and observation group by random number table method, with 43 cases in each group. The control group was treated with quadruple therapy and the observation group was treated with compound lactobacillus plus the same quadruple therapy as the control group. The two groups were treated for 2 weeks, and the Hp eradication rate was observed after 4 weeks of treatment. Results　The Hp eradication rate in the observation group was approximately the same as that in the control group(90.70% vs 86.05%), with no statistically significant difference between the two groups(P>0.05). There were 2 cases(4.65%) and 8 cases(18.60%) of diarrhea, 3 cases(6.98%) and 10 cases(23.26%) of abdominal distension in the observation group and the control group, respectively, with significant differences between the two groups(P<0.05). However, there was no significant difference in nausea symptom between the two groups(P>0.05). Conclusion　Although the combination of compound lactobacillus and standard quadruple therapy can not significantly improve the eradication rate of Hp, it can reduce the adverse reactions such as diarrhea and abdominal distension, with good safety.]]> 2019/9/30 8:42:00 CHEN Zhi-xin, LAI Xiang-li, HU Hua-hua 181true ［Abstract］　Objective　To study the clinical effect of plowing method on separating moderate to severe intrauterine adhesions. Methods　Sixty-eight patients with moderate to severe intrauterine adhesions were selected in our hospital from August 2016 to August 2018. The patients were randomly divided into control group and study group by random number table method, with 34 cases in each group. The patients in the control group were treated with hysteroscopy separation of uterine cavity adhesion by electric energy instrument, while the patients in the study group received the 3 mm rigid single-joint scissors in the HEOS non-energy hysteroscopy for plowing separation of intrauterine adhesions. The operation time, intraoperative bleeding, postoperative reexamination results of uterine cavity using hysteroscopy, postoperative menstrual changes and postoperative reproductive prognosis were compared between the two groups. Results　The operation time of the study group was significantly shorter than that of the control group(P<0.05). The volume of intraoperative bleeding of the study group was significantly less than that of the control group(P<0.05). The menstrual blood of study group was significantly more than that of control group(P<0.05). The curative effect of the study group was significantly better than that of the control group(P<0.05). The degree of intrauterine adhesion 6 months after operation in the study group was significantly less than that in the control group(P<0.05). During the follow-up period, the recurrence rate of the study group was significantly lower than that of the control group(P<0.05). The pregnancy rate of the study group was significantly higher than that of the control group(P<0.05). Conclusion　Using the 3 mm rigid single-joint scissors in the HEOS non-energy hysteroscopy for plowing separation of intrauterine adhesions has a better effect, which can not only reduce the secondary injury to the endometrium, but also improve the long-term treatment effect, reduce the recurrence rate, and improve the probability of pregnancy in the patients, which is safe and widely recommended in clinical practice.]]> 2019/9/30 8:42:00 RUAN Yu-ling, LI Ben-ben, LIU Mei 180true ［Abstract］　Objective　To knock out follistatin-like protein 1(FSTL1) gene in SiHa cells using CRISPR/Cas9 technology and to construct SiHa cell stable strain with knocking out FSTL1 gene. Methods　The sgRNA-FSTL1 recombinant plasmid was constructed and was packaged into lentivirus. The virus solution was collected and infected with SiHa cells, and SiHa cell stable strain with knocking out FSTL1 gene was screened by puromycin. The expressions of FSTL1 mRNA and protein in SiHa cell stable strain were detected by real-time quantitative polymerase chain reaction(RT-qPCR) and Western blot. Results　FSTL1-sgRNA lentivirus was successfully constructed and the SiHa cell stable strain with knocking out FSTL1 gene was successfully screened. The expressions of FSTL1 mRNA and protein in the SiHa cell stable strain group were significantly lower than those in the control group（P<0.05）. Conclusion　SiHa cell stable strain with knocking out FSTL1 gene is successfully constructed using CRISPR/Cas9 technology.]]> 2019/9/2 14:45:32 ZHANG Hong, HU Xiao-xia, LUO Ruo-yu, et al. 179true ［Abstract］　Objective　To evaluate the efficacy and safety of topical timolol in treatment of infantile hemangiomas(IH) and to provide evidence-based basis for the clinical medication. Methods　A comprehensive computer-based search of VIP, WanFang Data, CNKI, CBM, PubMed, Embase, Cochrance library and Medline was conducted to collect the randomized controlled trials(RCTs) about the treatment of IH with topical timolol from the date of inception to January 2019, and each study was screened strictly according to the inclusion criteria to perform a meta-analysis using the software RevMan 5.3. The literature quality and the risk of bias were assessed in all included references, and the efficacy and safety of timolol in IH treatment were explored. Results　Eleven literatures were included and the meta-analysis results were as follows: （1） Timolol was more effective than placebo and blank control［RR=2.79, 95%CI(2.13, 3.65), P<0.01］, and there was no significant difference in adverse reactions among the groups［RR=2.46, 95%CI(0.40, 15.22), P=0.33］; （2）There was no significant difference in effective rate between timolol and propranolol［RR=1.08, 95%CI(0.88, 1.31), P=0.47］, but timolol had a lower incidence of adverse reactions than propranolol［RR=0.29, 95%CI(0.19, 0.46), P<0.01］. Conclusion　Data analysis based on existing data shows that the topical treatment of timolol for IH is significantly effective, and there is no significant difference in effective rate between timolol and propranolol, but timolol has a lower incidence of adverse reactions.]]> 2019/9/2 14:45:32 HU Teng, FENG Hong-chao, SHAO Min 178true ［Abstract］　Objective　To investigate the clinicopathologic features and immunophenotypes of female genital tract Müllerian adenosarcoma(FGTMA) in 9 cases and to improve understanding of the disease. Methods　A retrospective study was conducted on the clinicopathologic features and immunophenotypes of FGTMA in 9 cases. All the patients received postoperative follow-up. Results　The major clinical symptoms of FGTMA were irregular vaginal bleeding, pelvic masses and cervical lesions. All the patients received surgical treatment, among whom 5 cases received adjuvant postoperative chemotherapy and 1 case received radiotherapy. Histopathologically, the tumors were composed of benign glandular epitheliums and malignant mesenchymal tissues. The sarcomatousstroma contained heterologous elements in 5 cases, among whom 4 cases had concomitant sarcomatous overgrowth. Immunohistochemically, glandular epitheliums showed expression of cytokeratin(CK) and mesenchymal tissues showed vimentin expression in all the cases, among whom 2 cases showed strong expression of P53(more than 75% cells with scattered positive expression), and 5 cases showed P16 expression, and 1 case was negative to MutS homolog 2(MSH2), MutS homolog 6(MSH6) and Mut L homologue 1(MLH1). The proliferation index of Ki-67 was 5%～45%. Conclusion　There are no specific clinical features of FGTMA. Accurate diagnosis of the disease relies on pathological examination. Patients receiving early diagnosis and complete surgical treatment have better outcomes than those with advance disease and sarcomatous overgrowth.]]> 2019/9/2 14:45:32 CHEN Hui-ling, MO Xiang-lan 177true ［Abstract］　Objective　To investigate the changes of occlusal plane and mandibular plane before and after orthodontic treatment in patients with skeletal class Ⅲ malocclusion of permanent dentition and their correlation. Methods　Twelve patients with skeletal class Ⅲ malocclusion permanent teeth were selected as the study subjects and their clinical data were retrospectively analyzed. The mean of ANB angle was (-3.33±1.74)°, and the mean of SN-MP angle was (37.67±4.89)°. The second or third permanent mandibular molars were extracted and treated with micro-implant anchorage or Multiloop Edgewise Arch-Wire. The changes of cephalometric parameters were compared before and after treatment and the correlations between the changes of maxillofacial surface and the changes of occlusal plane and mandibular plane were analyzed before and after treatment. Results　After correction, the ANB angle increased by (1.79±1.62)degrees, and the OPP-FH angle decreased by (2.83±2.92)degrees, and the FH-OP angle decreased by (2.25±2.13)degrees, with significant differences before and after treatment(P<0.05). There were no significant differences in OPA-OPP, FH-MP and SN-MP between preoperative treatment and postoperative treatment(P>0.05). Correlation analysis showed that there was a positive correlation between the changes of anterior height and anterior-inferior height and the changes of mandibular plane(P<0.05). Conclusion　As long as the anchorage design is reasonable, the skeletal class Ⅲ malocclusion can be corrected, and the patients′ normal profile can be improved to a certain extent, and the occlusal plane and the mandibular plane can be well controlled.]]> 2019/9/2 14:45:32 CHEN Su-juan, ZHOU Yan, HUANG Min-fang, et al. 176true ［Abstract］　Objective　To observe the therapeutic effect of hyperbaric oxygen therapy combined with vacuum sealing drainage（VSD） on patients with hard-healing wound of traumatic infections in extremities. Methods　Forty patients with hard-healing wound of traumatic infections in extremities 3 weeks after limb trauma who were treated in our hospital from January 2016 to January 2018 were seleeted, and they were randomly divided into the hyperbaric oxygen group(receiving hyperbaric oxygen therapy combined with VSD and surgical therapy) and the control group (receiving surgical therapy plus VSD) according to different treatments, with 20 cases in each group. The changes of wound area, Visual Analogue Scale(VAS) scores, and white blood cell(WBC), C-reactive protein(CRP) and platelets(PLT) were compared between the two groups before and 24 days after treatment（the equivalent of hyperbaric oxygen therapy for 20 times）. The positive results of wound bacteria culture after dressing changes were compared between the two groups on day 12 and day 24 of treatment. Results　Compared with those before treatment, the wound area, VAS scores, WBC, CRP and PLT improved significantly after treatment in the two groups, but the improvements of the above indicators in the hyperbaric oxygen group were better than those in the control group(P<0.05). The positive results of wound bacteria culture on the 12th day of treatment were not significantly different between the two groups(P>0.05), but there were significant differences in the positive results of wound bacteria culture on the 24th day of treatment between the two groups(P<0.05). Conclusion　Hyperbaric oxygen therapy combined with VSD therapy can accelerate the recovery of hard-healing wound of traumatic infections in extremities after limb trauma and has great practical value.]]> 2019/9/2 14:45:32 QIN Hui-yue, CHEN Huan 175true ［Abstract］　Objective　To explore the correlation between the characteristics of contrast-enhanced ultrasonography(CEUS) and molecular biological indicators and axillary lymph node metastasis in breast cancer. Methods　Retrospective analysis of the characteristics of CEUS and medical records were performed in 109 female patients with breast cancer. The correlation between the CEUS(including enhancement order, enhancement degree, filling defect, peripheral vascular convergence and enlargement of the enhancement area) and the molecular biological indicators and axillary lymph node metastasis was analyzed. Results　Of the 109 patients with a total of 109 lumps, CEUS revealed a filling defect in 68 cases, pathological findings of axillary lymph node metastasis in 64 cases, and Kappa consistency test showed a good consistency between the two examination methods(Kappa=0.732). The sensitivity of filling defect in diagnosis of axillary lymph node metastasis by CEUS was 92.19 % and the specificity was 83.33%. Conclusion　The filling defect of CEUS has a certain correlation with axillary lymph node metastasis in breast cancer. It can be used to evaluate the prognosis of breast cancer.]]> 2019/9/2 14:45:32 ZHOU Chun-qiao, WANG Xiao-yan, LI Xin-yan 174true ［Abstract］　Objective　To observe the anesthetic effect of ketamine combined with propofol on cynomolgus monkeys. Methods　Twenty-four male cynomolgus monkeys, aged 4～7 years, were selected and randomly divided into calf serum group, carbon tetrachloride group, alcohol group and control group, with 6 cases in each group. The monkeys were anesthetized with ketamine as basic anesthesia plus propofol intravenous anesthesia after 18 weeks of modeling and gunshot liver biopsy was performed on them. The vital signs were monitored during operation and the clinical anesthetic effect was observed, and statistical analysis was performed. Results　Liver fibrosis occurred in all the three model groups after 18 weeks of modeling, but it was most obvious in the carbon tetrachloride group. The onset time of ketamine intramuscular injection in the 24 cynomolgus monkeys was (4.20±1.55)min, and there was no significant difference in the onset time among the groups(P>0.05). The stimulating eye opening time in the 24 cynomolgus monkeys was (6.50±2.84)min, and there was no significant difference in the stimulating eye opening time among the groups(P>0.05). The heart rate and breathing were steady in the four groups receiving ketamine combined with propofol for anesthesia, and there were no significant differences in the end-tidal carbon dioxide pressure and heart rate before and after liver puncture in intra-group comparison and in comparison of the three model groups with the control group(P>0.05). The changes of blood sugar were obvious before and after liver puncture in the four groups, and there were significant differences in the blood sugar levels before and after liver puncture in intra-group comparison(P<0.05). However, there were no significant differences in the blood sugar levels between the three model groups and the control group(P>0.05). There were no restlessness, howling, rapid recovery in the anesthesia process of the four groups, and no adverse reactions such as vomiting and convulsion after operation. Conclusion　Carbon tetrachloride can be used to effectively establish the model of hepatic fibrosis in cynomolgus monkeys. Ketamine basic anesthesia plus propofol intravenous anesthesia has a good anesthetic effect on cynomolgus monkeys, which has a popularization significance.]]> 2019/9/2 14:45:32 LI Ping-ping, XIE Li-ping, WEI Zhu-mei, et al. 173true ［Abstract］　Objective　To observe the efficacy of extracorporeal shock wave lithotripsy combined with extracorporeal physical vibration lithotripsy in treatment of renal calculi. Methods　Eigty patients with renal calculi who were treated in our hospital from January 2016 to January 2018 were selected as the study subjects. The 80 patients were divided into observation group and control group by random number table method, with 40 cases in each group. The control group received routine extracorporeal shock wave lithotripsy, while the observation group received extracorporeal shock wave lithotripsy combined with extracorporeal physical vibration lithotripsy. The effect of lithotripsy and the general operation conditions after operation were compared between the two groups. Results　The stone removal rate in the observation group was significantly higher than that in the control group 2, 3 and 5 weeks after operation(P<0.05), and the stone exhaustion time, pain relief time and hospitalization time in the observation group was significantly shorter than that in the control group(P<0.05). The incidence of complications after operation in the observation group was also significantly lower than that in the control group(P<0.05). Conclusion　Extracorporeal shock wave lithotripsy combined with extracorporeal physical vibration lithotripsy is effective in treatment of renal calculi. It can effectively promote the rapid removal of calculi after operation.]]> 2019/9/2 14:45:32 ZHU Jin-yan, SHANG Cui-qing, ZHANG Chuan-hua, et al. 172true ［Abstract］　Objective　To study the expressions of connective tissue growth factor(CTGF) gene in multiple myeloma(MM) and normal tissues and their clinical significances. Methods　Datasets about CTGF in Oncomine database were retrieved and the role of CTGF in MM was analyzed by Meta analysis. Results　A total of 456 studies on the expressions of CTGF in different tumors were collected in the Oncomine database. Compared with those in the normal tissues, the CTGF expressions were significantly different in 52 studies among which the CTGF expressions were higher in 28 studies and lower in 24 studies. Eight datasets in the four studies included 379 samples involving the expressions of CTGF in the MM and normal tissues. Compared with those in the normal tissues, the expressions of CTGF in the cancer tissues were significantly higher in 5 datasets(P<0.05), but there were no significant differences in 2 datasets(P>0.05), in which the statistic value of CTGF was P=1.000 in 1 dataset. Meta analysis showed that there was no significant difference in the expression of CTGF between the cancer and normal tissues(P>0.05); Further analysis showed that there was no correlation between CTGF expression and prognosis(P>0.05). Conclusion　There is no significant difference in CTGF expression between normal and MM tissues. CTGF can not be used as a potential marker of MM to predict the prognosis of MM.]]> 2019/8/5 16:14:38 LIU Hong-wen, CHEN Jin-cheng, ZHU Guo-tao, et al. 171true ［Abstract］　Objective　To investigate the diagnostic value of endoscopic blue laser imaging(BLI) technology and iodine staining for multiple primary esophageal carcinoma. Methods　Four hundred and thirty-seven patients who were pathologically diagnosed with esophageal cancer at the Digestive Endoscopy Center of the People′s Hospital of Guangxi Zhuang Autonomous Region from June 2015 to August 2018 were selected. The diagnostic value of endoscopic BLI and iodine staining for multiple primary esophageal carcinoma was compared. Results　A total of 47 cases of multiple primary esophageal cancer were confirmed by pathology, accounting for 10.75%(47/437) of all the esophageal cancer patients. Ninety-nine lesions were detected, including 24 in the upper esophagus, 58 in the middle and 17 in the lower esophagus. There were 27 cases of early esophageal cancer and 72 cases of advanced esophageal cancer. The sensitivity and specificity of BLI in diagnosis of multiple primary esophageal cancer were 89.36% and 99.49%, respectively. The sensitivity and specificity of iodine staining in diagnosis of multiple primary esophageal cancer were 95.74% and 98.97%, respectively. BLI and iodine staining under endoscopy had good consistency with pathological diagnosis in diagnosis of multiple primary esophageal carcinom(Kappa=0.914,Kappa=0.930). However, there was no significant difference in the detection rate of multiple primary esophageal carcinoma between BLI and iodine staining(P>0.05). Conclusion　Both BLI and iodine staining under endoscopy have high diagnostic value for multiple primary esophageal carcinoma.]]> 2019/8/5 16:14:38 YI Nan, NONG Bing, ZHANG Wen-hua, et al. 170true ［Abstract］　Objective　To explore the effects of gross hematuria on the results of routine urine analysis. Methods　Modeling hematuria was performed to observe its effects on routine urine analysis before and after centrifugation. Results　Some parmeters appeared false positive results when the red blood cell concentration reached 100×10⁹/L. The effects of leukocyte esterase and ketone body on the results of routine urine analysis could be almost eliminated after centrifuging the urine, but the effects on protein false positive results could not be eliminated. Conclusion　Gross hematuria can impact some parmeters of routine urine analysis, and some interferences can be eliminated after centrifuging the urine. The visible components of the urine should be subject to the results of microscopic examination.]]> 2019/8/5 16:14:38 LI Li-xiu, YANG Zhi-yong, LIU Gui-zhi, et al. 169true ［Abstract］　Objective　To explore the changes of serum vaspin levels in patients with gestational diabetes mellitus(GDM) and its correlation with inflammatory factors of tumor necrosis factor-alpha(TNF-α) and interleukin-6(IL-6). Methods　One hundred and twenty pregnant women undergoing prenatal examination in the Obstetrical Out-patient Clinic of Heze Municipal Hospital from October 2016 to December 2017 were selected. The 120 pregnant women were divided into GDM group(n=60) and normal glucose tolerance(GNGT) group(n=60) according to their different oral glucose tolerance test(OGTT) results. The levels of fasting serum vaspin, TNF-α and IL-6 were detected by enzyme-linked immunosorbent assay(ELISA), and statistical analysis was conducted. Results　The serum levels of vaspin, TNF-α and IL-6 in the GDM group were significantly higher than those in the GNGT group(P<0.05).Correlation analysis showed that vaspin was positively correlated with pre-gestational body mass index(BMI), increment of BMI during pregnancy, fasting insulin(FINS) and homeostasis model assessment of insulin resistance(HOMA-IR) but negatively correlated with TNF-α and IL-6（P<0.05）. In a multiple linear regression analysis，pre-gestational BMI, TNF-α, IL-6 and HOMA-IR were independent risk factors of serum vaspin concentrations. Conclusion　Serum levels of vaspin, TNF-α and IL-6 in GDM patients are increased. Vaspin may be compensatory in the occurrence and development of obesity, insulin resistance and GDM, and can inhibit the inflammatory response.]]> 2019/8/5 16:14:38 ZHU Hui-jing, ZHU Xin, XIE He-lan, et al. 168true ［Abstract］　Objective　To investigate the application value of continuous thoracic paravertebral nerve block combined with dexmedetomidine intraveous analgesia in surgical anesthesia for elderly patients with breast cancer. Methods　Ninety-six elderly breast cancer patients who were treated with surgery in our hospital from January 2016 to December 2017 were selected. The patients were randomly divided into observation group(n=48) and control group(n=48). The control group was anesthetized by local infiltration and intravenous analgesia, and the observation group was anesthetized by ultrasound-guide continuous paravertebral nerve block combined with dexmedetomidine intravenous analgesia. The general indexes, anaesthesia effect and postoperative recovery were compared between the two groups. Results　There were no significant differences in age, weight, operation time, intraoperative bleeding volume and fluid infusion volume between the two groups(P>0.05), but the dosages of propofol and remifentanil in the observation group were significantly lower than those in the control group(P<0.05). There was no significant difference in CVP level of general hemodynamics between the two groups at different time phases(P>0.05), but the MAP and HR level at T₂, T₃ and T₄ phases in the observation group were significantly lower than those in the control group(P<0.05), and there were no significant differences in precise hemodynamic indexes(P>0.05). The resting VAS, active VAS and Ramsay Sedation Scores at 2 h, 6 h and 12 h after operation in the observation group were significantly better than those in the control group(P<0.05). The incidence rates of postoperative adverse reactions and complications in the observation group were significantly lower than those in the control group(P<0.05). Conclusion　The application of continuous thoracic paravertebral nerve block combined with dexmedetomidine in surgery for elderly patients with breast cancer can improve the anesthetic effect during operation and keep the hemodynamics and circulatory system of the patients stable during operation.]]> 2019/8/5 16:14:38 JIN Lian-jin, LI Li, ZHANG Li, et al. 167true ［Abstract］　Objective　To explore the effects of masked hypertension（MH） on cardio-cerebral vascular events in patients with coronary heart disease complicated with metabolic syndrome. Methods　A total of 66 patients with coronary heart disease complicated with metabolic syndrome proven by coronary angiography in our hospital from August 2015 to August 2017 were selected and divided into normotension group（n=32） and MH group（n=34） according to their 24-hour ambulatory blood pressure monitoring results. The difference in the parameters of 24-hour ambulatory blood pressure was compared between the two groups and the major adverse cardio-cerebral vascular events（MACCE） were compared between the two groups after a follow-up. Results　The body mass index（BMI） and waist circumference in the MH group were higher than those in the normotension group（P<0.05）. The levels of 24-hour mean systolic blood pressure（24hSBP）, daytime mean systolic blood pressure（dSBP）, nighttime mean systolic blood pressure（nSBP）, daytime systolic blood pressure（SBP） plateau, nighttime SBP plateau and increasing rate of morning SBP in the MH group were significantly higher than those in the normotension group（P<0.05）. Univariate correlation analysis showed that 24hSBP was positively correlated with waist circumference and BMI（P<0.05）. Logistic regression analysis showed that larger waist circumference and BMI were the risk factors of MH（P<0.05）. Kaplan-Meier survival curves showed that MACCE was significantly higher in the MH group（P<0.05）. Multivariable Cox regression analysis showed that the higher level of 24hSBP and suffered with MH were the risk factors of MACCE（P<0.05）. Conclusion　Overweight and obesity may raise the level of blood pressure and aggravate morning blood pressure fluctuations. The elevated levels of 24hSBP may be an important risk factor of MACCE in patients with coronary heart disease complicated with metabolic syndrome.]]> 2019/8/5 16:14:38 XU Yao, HAO Yun-xia, SUI Ying-li, et al. 166true ［Abstract］　Objective　To explore the acceptability of the test results between two kinds of blood cell analyzer using the Clinical and Labratory Standards Institute(CLSI) EP9-A3 files. Methods　Refering to CLSI EP9-A3 files and Sysmex XE-2100 blood cell analyzer as reference system, we detected 40 fresh anticoagulated whole blood samples respectively, using Sysmex XE-2100 and Mindary BC-5800 blood cell analyzers in a normal condition. The detection results of white blood cell(WBC), red blood cell(RBC), hemoglobin(Hb), platelets(PLT) and mean corpuscular volume(MCV) were compared between the two system. The outliers inspection and offset were evaluated by ESD method. Choose the best regression model fitting regression equation to calculate the 95% confidence interval(CI) and medical decision level of each item. 1/2 CLIA′88 permitted error was taken as a standard to determine whether a deviation was acceptable. Results　The test results of WBC and MCV could not be comparable between the two kinds of blood cell analyzer, but the test results of RBC, Hb and PLT can be comparable between the two kinds of blood cell analyzer. Conclusion　CLSI EP9-A3 can be used to compare different kinds of blood cell analyzer, and different kinds of blood cell analyzer should be compared regularly in the same laboratory to provide reliable test results for clinical applications.]]> 2019/8/5 16:14:38 SU Hang-jiu, ZHOU Cheng-yan, YANG Wen-hui 165true ［Abstract］　Objective　To investigate the correlation of plasma brain natriuretic peptide(BNP) and the right ventricular function in patients with tetralogy of Fallot(TOF). Methods　From February 2016 to June 2017, 30 patients with TOF in our hospital were enrolled as the reseach group, and other 30 patients with asymptomatic ventricular septal defect were enrolled as the control group. Both of the two groups underwent surgery. The plasma BNP were recorded in the two groups before operation and 1 week, 1 month and 3 months after operation. The right ventricular end-diastolic volume(RVEDV), right ventricular anterior wall thickness(RVAW), ratio of peak blood flow filling velocity in early and late diastolic period of right ventricle(E/A), left ventricular ejection fraction(LVEF) and Tei-index in the reseach group were recorded before and after operation. Results　The levels of plasma BNP in the reseach group were significantly higher than those in the control group before operation and 1 week, 1 month and 3 months after operation（P<0.05）. In the reseach group, the levels of BNP, RVEDV, RVAW, E/A, LVEF and Tei-index were gradually decreased with the time going from preoperation to 1 week, 1 month and 3 months after operation（P<0.05）. The results of Pearson correlation analysis showed that the levels of BNP were significantly positively correlated with the levels of RVEDV, RVAW, E/A and Tei-index at 1 week, 1 month and 3 months after operation（P<0.05）. Conclusion　The level of plasma BNP is obviously higher in the patients with TOF, and the BNP level is closely related to the right ventricular function.]]> 2019/8/5 16:14:38 LING Yi, DENG Hai-qing 164true ［Abstract］　Objective　To explore the relationship between serum 25-hydroxy vitmin D［25(OH)D］ levels and the occurrence of poststroke cognitive impairment(PSCI) in elderly patients. Methods　Three hundred and sixty-eight elderly cerebral infarction patients who were over 60 years old were selected in our hospital from January 2015 to December 2017, and were divided into non-poststroke cognitive impairment(NPSCI) group(n=253) and PSCI group(n=115) according to the Montreal Cognitive Assessment Scale(MoCA). Thirty elderly people from physical examination were selected as normal control group. The levels of serum 25(OH)D were compared among the three groups, and the risk factors of PSCI were analyzed by Logistic regression analysis. Results　The serum 25(OH)D levels in both the PSCI group and the NPSCI group were significantly lower than those in the normal control group(P<0.05). In addition, the PSCI group had lower levels of serum 25(OH)D compared to the NPSCI group(P<0.05). Logistic regression analysis results showed that lower serum 25(OH)D level, old age, male, history of hypertension, history of diabetes, and history of strok were the independent risk factors of PSCI. Conclusion　Serum 25(OH)D level is associated with the occurrence of PSCI. Low serum 25(OH)D level may be an independent risk factor for PSCI in elderly patients.]]> 2019/7/1 16:32:50 YANG Hong-wen, TANG Chun-qiong, OU Jia-huan, et al. 163true ［Abstract］　Objective　To explore the characteristics and significances of perineural invasion(PNI) in oral tongue squamous carcinoma with different clinical pathological parameters. Methods　The PNI characteristics of 29 oral tongue squamous cell carcinoma patients with different tumor differentiations, T stages and lymph node metastasis were observed, and the relationship between different clinical parameters and PNI was analyzed. Results　There was no significant difference between the number of nerve bundles in the normal tissue and the tongue squamous carcinoma tissue(P>0.05). The PNI rate was significantly higher in the moderately and poorly differentiated squamous cell carcinoma than that in the well-differentiated squamous cell carcinoma(P<0.05). There was no significant difference in PNI rate among the patients at different T stages(P>0.05). The PNI rate of the patients with lymphatic metastatic squamous cell carcinoma was significantly higher than that of the patients with no lymphatic metastatic squamous cell carcinoma(P<0.05). Conclusion　No regenerative nerve bundles are found in the tongue squamous cell carcinoma tissues. The neurotrophic properties of the moderately and poorly differentiated oral tongue squamous cell carcinoma are higher than those of the well-differentiated squamous cell carcinoma, and the neurotrophic properties of the oral tongue squamous cell carcinoma with lymph nodemetasis are higher than those without lymph node metastasis.]]> 2019/7/1 16:32:50 LI Rou-xi, WU Man-man, CHEN Jun-wen, et al. 162true ［Abstract］　Objective　To study the diagnostic value of magnifying endoscopy and narrow-band imaging(M-NBI) in diagnosis of low grade adenoma and early carcinoma of gastric superficial elevated gastric lesions. Methods　From September 2016 to May 2018, 53 cases of superficial protuberance lesions of stomach were examined by painless gastroscopy in the Second Affiliated Hospital of Zhengzhou University, who were selected into this study. Diagnostic tests were performed on a patient using white light imaging(C-WLI) and M-NBI, and we determined the sensitivity, specificity and accuracy of C-WLI and M-NBI. Results　The sensitivity, specificity and accuracy of C-WLI were 64.0%, 90.0% and 72.0%, respectively. The sensitivity, specificity and accuracy of M-NBI were 94.0%, 90.0% and 93.0%, respectively. Conclusion　M-NBI is helpful in differentiating cancerous superficial elevated lesions of the stomach from adenoma.]]> 2019/7/1 16:32:50 LIU Shu-hong, ZHOU Ying-fa, JIA Meng-meng, et al. 161true ［Abstract］　Objective　To evaluate the effects of time-lapse culture and conventional embryo culture on the development of embryos. Methods　The embryo development data of 593 in-vitro fertilization(IVF)/intracytoplasmic sperm injection(ICSI) cycles(including 351 cycles in TL group and 242 cycles in CO group) were retrospectively analyzed. The fertilization rate, cleavage rate, good embryo rate, frozen embryo rate, available embryo rate, blastocyst formation rate, good blastocyst rate and frozen blastocyst rate were compared between the two groups. Results　(1)There were no significant differences between the two groups in female age, duration of infertility, retrieved oocytes and MII oocytes(P>0.05). (2)There were no significant differences in 1PN(3.14% vs 3.54%), ≥3PN(7.36% vs 8.74%) and unknown fertilization rate(2.16% vs 2.75%) between the two groups(P>0.05), but 2PN fertilization rate in TL group was significantly higher than that in CO group(71.44% vs 64.07%)(P<0.05). (3)There were no significant differences in the total cleavage rate(98.26% vs 98.54%), 2PN cleavage rate(98.84% vs 98.90%) and available embryo rate(78.69% vs 77.30%) between the two groups(P>0.05), but the good embryo rate and the frozen embryo rate of TL group were significantly higher than those of CO group(45.21% vs 41.92%, 62.16% vs 54.47%)(P<0.05). (4)There were no significant differences in the blastocyst formation rate, good blastocyst rate and the frozen blastocyst rate between the two groups(64.71% vs 67.80%, 20.59% vs 19.42%, 33.79% vs 34.12%)(P>0.05). Conclusion　Time-lapse culture does not have a significant adverse impact on the development of embryos, and can provide more information on embryo development for the selection of embryos.]]> 2019/7/1 16:32:50 WANG Shi-kai, XUE Lin-tao, TAN Wei-hong, et al. 160true ［Abstract］　Objective　To observe the efficacy of MORA biophysical therapeutic instrument combined with levocetirizine in treatment of chronic urticaria, and to analyze the changes in serum histamine concentration in different treatment groups before and after treatment. Methods　Ninety chronic urticaria patients were randomly divided into instrument group, drug group and combination group, with 30 cases in each group. After 12 weeks of treatment, the efficacy and the concentration of histamine in serum were compared among the three groups. Results　After treatment, the serum histamine concentrations of the three groups were significantly lower than those before treatment(P<0.05), and the serum histamine concentrations of the combination group were significantly lower than those of the other two groups(P<0.05). The total effective rates were 96.67%, 70.00% and 66.67% respectively in the combination group, the instrument group and the drug group, with a significant difference among them(P<0.05). Conclusion　Compared with single MORA biophysical treatment and single levocetirizine treatment, the combination of MORA biophysical therapeutic instrument and levocetirizine has a better efficacy on chronic urticaria and significantly reduces the concentration of histamine in serum.]]> 2019/7/1 16:32:50 LI Jian-min, TAN Mei-le, YANG Meng, et al. 159true ［Abstract］　Objective　To evaluate the diagnostic efficiency ability of confocal microscopy(CM) for pathogens of four kinds of infectious keratitis caused by bacteria, fungi, herpes simplex virus and acanthamoeba. Methods　We selected the patients diagnosed with the four kinds of infectious keratitis in our hospital. CM was performed to differentially diagnose the pathogens by blind method to analyse the diagnostic efficiency. Results　One hundred and fifty-one cases were diagnosed with infectious keratitis, including 43 cases of bacterial keratitis, 56 cases of fungal keratitis, 49 casese of herpes simplex virus keratitis and 3 cases of acanthamoeba keratitis. The sensitivities of CM in diagnosis of bacterial keratitis, fungal keratitis, herpes simplex virus keratitis and acanthamoeba keratitis were 76.74%, 91.07%, 77.56% and 100.00% respectively, and the specificities of the four infections were 85.19%, 97.89%, 92.16% and 100.00% respectively. The Kappa values of the four infections were 0.59, 0.90, 0.71 and 1.00 respectively. Conclusion　CM has high efficiency in differential diagnosis of pathogens of infectious keratitis.]]> 2019/7/1 16:32:50 LAN Qian-qian, CHEN Li-fei, HUANG Hui, et al. 158true ［Abstract］　Objective　To investigate the risk factors of Henoch-Schönlein purpura nephritis(HSPN) in children. Methods　The clinical data and related laboratory test results of 106 children with Henoch-Schönlein purpura(HSP) who were hospitalized in our department from January 1 2013 to December 31 2017 were collectded and analyzed. These patients were divided into HSPN group and HSP without nephritis(HSPWN) group, and case-control study design was used to compare the relevant exposure factors between the two groups. Results　A total of 106 patients were enrolled, among whom there were 66 males and 40 females with a median age of 7 years. These patients were divided into the HSPN group(n=39) and the HSPWN group(n=67) according to the HSPN diagnostic criteria in the Guidelines for the Diagnosis and Treatment of Purpura Nephritis(Trial) which was formulated and released by the Nephrology Group of the Pediatrics Society of the Chinese Medical Association in 2009. The incidence of HSPN was 36.79% in the HSP patients, including 24 males and 15 females, with median age being 9.50 years. The levels of total cholesterol, creatinine, uric acid, and cystatin C in the children with HSPN were significantly higher than those in the children with HSPWN（P<0.05）, while the level of creatinine clearance rate(Ccr) in the HSPN group was significantly lower than that in the HSPWN group（P<0.05）. Logistic multivariate regression analysis showed that the elevated level of total cholesterol was the risk factor of HSPN（OR=1.558, P=0.014）. Conclusion　The elevated level of total cholesterol is the risk factors for children with HSPN.]]> 2019/7/1 0:00:00 LI Xin-ye, DAI Yan, LU Yuan-feng, et al. 157true ［Abstract］　Objective　To summarize the clinical features of four patients with acute myocardial infarction(AMI) under 30 years old. Methods　The clinical data of 4 patients with AMI under 30 years old were retrospectively analyzed, and the relevant literatures at home and abroad were reviewed. Results　(1)All the four patients were male patients. (2)All the four patients had different degrees of smoking history, all of which showed high triglycerideemia or low-density lipoprotein cholesterol levels, and had a habit of high-calorie and high-salt diet. Two cases had a clear cause of the disease(lack of sleep, emotional agitation, etc.). (3)Two patients had no symptoms of angina pectoris before myocardial infarction. (4)The results of three cases of angiography had single-vessel disease, and two cases were anterior descending artery lesions. The infarct-related blood vessels were not completely occluded, and the most serious stenosis of case 2 was only 50%.There was no recurrence after regular reexamination, and the prognosis was good. Conclusion　Men, smoking history, obesity, lipid abnormalities, family history and environmental risk factors, emotional agitation and other unhealthy lifestyles maybe the main risk factors for AMI in young people, and some patients have atypical clinical manifestations.]]> 2019/7/1 16:32:50 SHI Xiang-xin, ZHAO Yue-hua, ZHANG Yun-huan, et al. 156true ［Abstract］　Objective　To evaluate the effectiveness and safety of negative suction combined ureteroscopy in treatment of upper ureteral stones with diameters more than 10 mm. Methods　The data of 145 patients with upper ureteral stones who were treated by ureteroscopic lithotripsy in our hospital during January 2016 and October 2018 were retrospectively investigated. These patients were divided into negative suction group(treated with negative suction combined ureteroscopy, n=97) and conventional therapy group(treated with conventional ureteroscopic holmium laser lithotripsy, n=48) according to different treatment methods. The stone clearance rate and infectious complication rate were compared between the two groups. Results　The stone clearance rate of the negative suction group was significantly higher than that of the conventional therapy group 4 weeks after operation（88.70% vs 68.75%）（P<0.05）.The incidence rates of infectious complications(T>38 ℃, ratio of cases with abnormal CRP/PCT increase after surgery) in the negative suction group were significantly lower than those in the conventional therapy group（9.28% vs 22.92%; 10.31% vs 25.00%; 8.25% vs 20.83%）(P<0.05). Conclusion　Negative suction combined ureteroscopy is safe, efficient and simple for the patients with upper ureteral stones with diameters more than 10 mm.]]> 2019/7/1 16:32:50 ZHANG Sui-bing, PAN Wei-bing, PAN Chang-li 155true ［Abstract］　Objective　To explore the diffusion tensor imaging(DTI) parameter features of cerebellar peduncles(CPs) and decussation of superior cerebellar peduncle(DSCP) in different age groups of healthy people. Methods　Sixty healthy adults were recruited for DTI examination and they were divided into group A(20～39 years), group B(40～59 years) and group C(60～79 years) according to different ages, with 20 cases in each group. The average diffusion coefficient(DCavg) and fractional anisotropy(FA) values of the CPs and DSCP were compared between the different cerebellar sides in the same group and among group A, group B and group C. Results　Overall, the DCavg and FA values of superior cerebellr pedunecles(SCP), middle cerebellar peduneles(MCP) and inferior cerebellar peduneles(ICP) were not significantly different on the left and right sides(P>0.05). There were significant differences in DCavg and FA values of DSCP, FA values of SCP, and DCavg values of ICP among different age groups(P<0.05). There were no significant differences in DCavg and FA values in other parts between among age groups(P>0.05). Conclusion　DTI can reflect the physiological changes of CPs and DSCP, and visually show the structure of the cerebellar pathway fiber bundle, providing more comprehensive information for the study of cerebellar lesions.]]> 2019/7/1 16:32:50 LONG Xiu-ling, XIA Zhen-yuan, DAI Yi, et al. 154true ［Abstract］　Objective　To summarize the clinical experience of surgical management of Stanford type A aortic dissection associated with pregnancy, and to evaluate its safety and efficacy. Methods　The clinical data of 2 patients with pregnancy complicated with Stanford type A aortic dissection who received surgical treatment from April 2012 to September 2018 in Sichuan Academy of Medical Sciences & Sichuan Provincial People′s Hospital were retrospectively analyzed. Both patients underwent aortic root replacement+Sun′s surgery+caesarean section at the same time. Results　Both mothers survived. Case 1 was complicated with postoperative pulmonary infection. Case 2 was complicated with extremity movement restriction of the right lower limb caused by acute spinal cord ischemia injury and sacrococcygeal bedsore. Both mothers were improved and discharged after active treatment, but 2 cases of fetus died. The two mothers were followed up for 2 to 11 months after operation. No obvious abnormality of the prosthetic valve in aortic valve position was found by transthoracic echocardiography. Aortic computed tomography angiogram(CTA) detected normal aortic and valvular structure, without signs of distal dilation and with organized thrombus in the aortic false lumen. Case 1 recovered well and went to work. The body strength of right lower limb in case 2 was restored to grade Ⅳ. Conclusion　Patients with pregnancy complicated with Stanford type A aortic dissection should be operated actively. The optimized surgical management should be decided according to the pathological changes of the aorta, gestational weeks, fetal intrauterine condition and the patients′ wish.]]> 2019/5/31 17:17:32 LIU Sheng-zhong, WEI Da-chuang, TAN Jin, et al. 153true ［Abstract］　Objective　To analyze the audiological characteristics and the curative effects of sudden deafness in the elderly people aged 60 years and above. Methods　Thirty-nine cases of senile sudden deafness, aged 60 to 89 years and hospitalized in Shunde Hospital of Southern Medical University from June 2017 to September 2018 were retrospectively analyzed, and their hearing loss levels were analyzed. The study subjects were divided into two groups: <75 years old group(n=25) and ≥75 years old group(n=14), and the curative effects were compared between the two groups. Results　The hearing loss was serious in the elderly people with sudden deafness, 2.5% with mild hearing loss, 12.8% with moderate hearing loss, 38.5% with severe hearing loss and 46.2% with extremely severe hearing loss. There were no significant differences in the classification levels of hearing loss between the <75 years old group and the ≥75 years old group(P>0.05). After treatment, 2 cases were cured, 4 cases markedly effective, 6 cases effective and 13 cases ineffective in the <75 years old group, while 0 cases were cured, 0 cases markedly effective 2 cases effective and 12 cases ineffective in the ≥75 years old group, with a significant difference in the curative effect between the two groups(P<0.05). Conclusion　The hearing loss of sudden deafness is serious in the elderly, and the proportion of severe and extremely severe hearing loss is high. The treatment effects are poor and decrease further with the increase of age.]]> 2019/5/31 17:17:32 ZHANG Bi-ru, XU Jia-bao, YANG Jia-ying, et al. 152true ［Abstract］　Objective　To separate and purify the main related substances of new sapogenin hydroxylation panoxadiol（HPD）, and to confirm their structures. Methods　Four main impurities were isolated from new sapogenin HPD using repeated silica column chromatography and recrystallization. The structural characteristics of the impurities were determined by physical and chemical properties and spectral methods, such as LC-MS, ¹H-NMR, and ¹³C-NMR. Results　The structrues of the four related substances for new sapogenin HPD were identified as (20S, 24R)-dammar-20, 24-epoxy-3β, 6α, 12β, 25-tetraol, (20S, 24S)-dammar-20, 24-epoxy-3β, 12β, 25-triol, (20S, 25R)-dammar-20, 25-epoxy-3β, 12β, 26-triol and dammar-24-ene-3β, 12β, 20S-triol. Conclusion　The quantitative and qualitative studies on the HPD related substances of new sapogenin HPD can provide a basis for the quality control of HPD active pharmaceutical ingredients.]]> 2019/5/31 17:17:32 GAO Lian-cong, MA Ying-hui, LI Ping-ya, et al. 151true ［Abstract］　Objective　To evaluate the effects of premolar extraction and non-tooth extraction correction on upper airway morphology and hyoid position in immature premature permanent occlusion of Anglel ClassⅠ maloocclusion by cephalometrics to guide the implementation of clinical correction. Methods　Sixty patients with immature premature permanent occlusion of Anglel ClassⅠ maloocclusion were seleted and the patients were divided into premolar extraction group and non-tooth group according to their different therapy methods, with 30 cases in each group. The changes of upper airway morphology and hyoid position were observed before and after the extraction treatment. Results　The differences in PNS-UPW, U-MPW, H-FH and H-VL measurement items were statistically significant in the non-tooth extraction group before and after treatment(P<0.05). The measurement items of PNS-UPW, SPP-SPPW, U-MPW and H-MP were significantly different in the premolar extraction group before and after the extraction treatment(P<0.05).There was a statistically significant difference in the measurement item of PNS-UPW between the non-tooth extraction and the premolar extraction group after treatment(P<0.05). There were no statistically significant differences in the other measurement items between the two groups(P>0.05). Conclusion　Angle ClassⅠ with premature permanent occlusion has no significant effect on the change in the upper airway morphology and has no obvious effect on hyoid position, but produces backward on hyoid position in the sagittal direction and has a downward movement trend.]]> 2019/5/31 17:17:32 CHEN Huan-huan, MA Kun-ning, ZHANG Zuo, et al. 150true ［Abstract］　Objective　To explore the expression and effect of interleukin 6(IL-6) in human immunodeficiency virus(HIV) and Talaromyces marneffei(TM) coinfection patients. Methods　Twenty-seven HIV-infected patients who had not received antiretroviral therapy(ART) were selected from the Fourth People′s Hospital of Nanning City, and were divided into HIV+TM group(12 cases) and HIV group(15 cases) according to the infection status of TM. The demographic data and the level of IL-6 in perpheral blood mononuclear cell(PBMC) were compared between the two groups. Results　There were no significant differences in age, gender and viral load between the two groups(P>0.05), while the CD4⁺ T cell count of the HIV+TM group was significantly lower than that of the HIV group(P=0.018). The IL-6 expression level of macrophages(a subgroup of PBMC) in the HIV+TM group was significantly higher than that of the HIV group(P=0.039). Conclusion　The IL-6 expression of HIV+TM group in PBMC is higher than HIV group, which may lead to immune hyperactivation in macrophages and may be a critical factor to promote TM infection and disease progression.]]> 2019/5/31 17:17:32 LUO Chao-lian, XIE Zhi-man, QIN Ying-mei, et al. 149true ［Abstract］　Objective　To establish a rapid and accurate method for the identification of bloodstream infection pathogens by matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS). Methods　Five hundred and twenty-four blood culture bottles with positive alarm by blood culture instrument were collected in the Department of Clinical Laboratory of the Affiliated Children′s Hospital of Zhengzhou University during July 2017 to December 2017. The blood was drawn from the blood culture bottles and moved to serum separation rubber tubes, where bacteria were enriched and collected using differential centrifugation. The enriched bacterial liquid was identified rapidly by MALDI-TOF MS, and the results of colony-forming units were compared with those of colony-forming units formed by traditional bacterial culture in 24 to 48 hours. Results　Of the 524 positive blood culture samples, 496 were single strain bloodstream infections, 16 mixed bacteria infections and 12 blood cultures false positive result of the alarm. Among the 496 single-strain bloodstream infections, the genus and species identification rates of the strains were 98.6%(489/496) and 95.8%(475/496) respectively. Among them, 120 strains were Gram-negative bacteria with a species identification rate being 99.2%(119/120) and 98.3%(118/120), and 376 strains were Gram-positive genus with a species identification rate being 98.4%(370/376) and 94.9%(357/376). No identification results were obtained in 16 cases of mixed bacteria infection and 12 blood cultures with false positive alarms. Conclusion　MALDI-TOF MS is used to directly identify the bacterial liquid after differential centrifugation. Compared with the traditional culture identification method, MALDI-TOF MS has higher identification accuracy rate of the main pathogens in bloodstream infections, and MALDI-TOF MS is a fast, simple and low-cost method. MALDI-TOF MS can effectively shorten the time of detection and has high accuracy of identification, and is suitable to be applied in a clinical microbiology laboratory.]]> 2019/5/31 17:17:32 FANG Pan-pan, YANG Jun-mei, YANG Jun-wen, et al. 148true ［Abstract］　Objective　To investigate the effects of different artificial shrinking methods before vitrified cryopreservation on survival rate and hatching efficiency in human blastocysts freeze-thaw. Methods　Two hundred and forty-six surplus blastocysts after vitrification or transplantation in IVF/ICSI cycles were divided into control group(with untreated blastocysts), laser drilling group(with artificial shrinking performed by a laser pulse prior to vitrification), and pipetting group(with mechanical extruding the blastocyst prior to vitrification), with 82 cases in each group. The survival rate and hatching rate were compared among the three groups. Results　The survival rate and the hatching rate in the laser drilling group and the pipetting group were higher than those in the control group(P<0.05). For good quality blastocysts, the hatching rates in the laser drilling group and the pipetting group were higher than those in the control group(P<0.05), but there were no significant differences in the proportions of the survival rate and the hatching rate between the laser drilling group and the pipetting group(P>0.05). For poor quality blastocysts, the survival rate and the hatching rate of the two artificial shrinking groups were higher than those of the control group(P<0.05), and the hatching rate of the laser drilling group was higher than that of the pipetting group(P<0.05). Conclusion　The outcome of blastocysts freeze-thaw can be significantly improved by artificial shrinkage, which is used to help blastocoele collapse prior to blastocyst vitrification. Laser pulse artificial shrinkage is demonstrated to be a more effective method for blastocyst vitrification.]]> 2019/5/31 17:17:33 ZHOU Ting-ting, XUE Lin-tao, WANG Shi-kai, et al. 147true ［Abstract］　Objective　To investigate the clinical effect of bionic electrical stimulation on promoting postoperative rehabilitation of induced abortion. Methods　From November 2015 to June 2016, 698 pregnant women who voluntarily requested induced abortion in the Outpatient Department of Obstetrics and Gynecology of the People′s Hospital of Liujiang District were collected and divided into treatment group(n=333) and control group(n=365). The control group received postoperative natural rehabilitation, while the treatment group received bionic electrical stimulation. The sum of three diameters of the uterus, urine pregnancy test, uterine hemorrhage, intrauterine residue, clot retention and the degree of pain were compared between the two groups. Results　The incidence rates of uterine hemorrhage, intrauterine residue and clot retention in the treatment group were significant lower than those in the control group(P<0.05). The degree of pain in the treatment group was significantly lighter than that in the control group 7 days after operation(P<0.05), however there was no significant difference in the degree of pain between the two groups 14 days after operation(P>0.05). The negative rate of urine pregnancy test in the treatment group was significantly higher than that in the control group 7 days after operation(P<0.05), and the sum of three diameters of the uterus in the treatment group was significantly lower than that in the control group 7 days after operation（P<0.05）. However there was no significant difference in the negative rate of urine pregnancy test between the two groups 14 days after operation（P>0.05）. Conclusion　Bionic electrical stimulation can effectively promote the early postoperative rehabilitation of the patients after induced abortion.]]> 2019/5/31 17:17:33 ZHANG Cui-lan, WEI Xiao-hong, XIAN Ju-fen, et al. 146true ［Abstract］　Objective　To analyze and predict the target genes of homo sapiens miRNA-613(hsa-miR-613) and their possible biological processes by using bioinformatics techniques, and provide a basis for further research on the relationship between microRNA and heart diseases. Methods　The basic data of hsa-miR-613 including chromosomal location, base sequence and conservation of species were obtained from miRbase database and UCSC Genome Browser. Target genes of hsa-miR-613 were predicted by miRDB, TargetScan, miRanda and DIANA-microT and then the intersection was performed. The target genes supported by literature and confirmed by experiments from miRTarbase database were combined as a target gene set, which was analyzed with Gene Ontology(GO) analysis and signal pathway analysis. Results　The sequence of hsa-miR-613 was highly conserved among virious species, and its target gene functions were enriched in biological processes such as vascular development, cell proliferation and development; target genes existed in various components of the cell such as cytoplasm, organelles, focal adhesion, etc and molecular functions were enriched in protein binding and transcription factor activities and so on(P<0.05). The signal pathway analysis showed that the target genes were enriched in the pathways including synaptic vesicle cycle, spliceosome, PI3K-Akt signaling pathway(PI3K-Akt), mTOR signaling pathway, focal adhesion, and other pathways including congenital diseases and cardiovascular diseases including congenital heart disease(P<0.05). Conclusion　The predicted target genes of hsa-miR-613 enrich in multiple biological processes and are related to cardiac development, which provides a biological basis for further research.]]> 2019/4/30 17:22:17 CHAO Xiao-ying, ZHONG Guo-qiang, JIANG Zhi-yuan, et al. 145true ［Abstract］　Objective　To investigate the oral health status of 12-year-old pupils in Zhuhai City, and analyze the related factors and to provide scientific evidence for oral health care. Methods　633 pupils aged 12 years old were selected as the research subjects in Zhuhai City from three primary schools by cluster ramdom sampling. Oral health checkups and questionnaires were carried out on them. Results　In the 633 pupils selected, the per capita dental caries was 0.87, and the caries prevalence rate was 37.28%. The gingival bleeding detection rate was 37.12%. Conclusion　The prevalence of dental carles in 12-year-old pupils in Zhuhai is higher. Non-frequency tooth brushing, eating before going to bed, and drinking carbonated drinks are the risk factors of children′s oral health. We need to strengthen the propaganda of oral health care knowledge.]]> 2019/4/30 17:22:17 LIU Jin-gang, LIN Ai-ying, CAI Jing 144true ［Abstract］　Objective　To explore the expression of long chain noncoding RNA-ROR(lncRNA-ROR) in the serum of the patients with chronic heart failure(CHF) and its clinical significance. Methods　The expressions of lncRNA-ROR in the serum of 25 healthy subjects(control group) and in 109 patients with CHF were detected by Real-time PCR. The correlation between the expressions of lncRNA-ROR and NT-proBNP, left ventricular end-diastolic diameter and left ventricular ejection fraction was analyzed. Results　The expression of lncRNA-ROR in the CHF patients was significantly higher than that in the control group（P<0.05）. The expression of lncRNA-ROR was positively correlated with cardiac functional grading of CHF, left ventricular end-diastolic diameter and NT-proBNP, and was negatively correlated with left ventricular ejection fraction（P<0.05）. Conclusion　The expression of lncRNA-ROR is related to the severity of heart failure. lncRNA-ROR is expected to be a biomarker for early diagnosis of CHF.]]> 2019/4/30 17:22:17 ZHANG Li-zhen, LIU Shu-zhen, MA Jun, et al. 143true ［Abstract］　Objective　To monitor the changes of neutrophil gelatinase-associated lipocalin(NGAL) in the serum of rats with acute kidney injury(AKI) and to explore the value of NGAL in the early diagnosis of AKI. Methods　Forty male Wistar rats were divided into sham-operation group, mild injury group, moderate injury group and severe injury group, with 10 cases in each group. The models of renal injury were made at different time points. Serum NGAL was detected at 0 h, 2 h, 6 h, 12 h and 24 h, and the diagnostic value of NGAL at different time points was analyzed. Results　(1)There were no significant differences in the NGAL levels among the groups with different degrees of renal injury(P>0.05). There were no significant differences in the NGAL levels between the sham-operation group and mild injury group at different time points(P>0.05), while the NGAL levels of the moderate injury group and the severe injury group were statistically different at different time points(P<0.05). There was no interaction between groups and time points(P>0.05). (2)AUCs were 0.855, 0.413, 0.210 and 0.370 at 2 h, 6 h, 12 h and 24 h respectively. Conclusion　The changes of NGAL show the same trend in the rats with moderate and severe AKI. NGAL expression peaks at 2 h after injury, which can be used as the best time point for early diagnosis of AKI. The ROC curve further shows that the time point of 2 h associated has great value in the early diagnosis of critical AKI in rats.]]> 2019/4/30 17:22:17 LI Xiu-yuan, GAO Jin-xia, HAN Peng-fei, et al. 142true ［Abstract］　Objective　To evaluate the curative effect and toxic and side effects between postoperative chemo-radiotherapy(ChRT) and chemotherapy(Ch) to detemine the better treatment options. Methods　Two investigators independently searched Pubmed, EMBASE, Cochrane library, CNKI,CQVIP and Wangfang Database(data span time from the time of establishing database to July 2018) for randomized controlled trials(RCTs) comparing postoperative ChRT with Ch. Twenty one RCTs comparing postoperative ChRT with Ch(n=2 299) were included and 2 299 patients were included. The primary outcomes were 3- and 5-year overall survival(OS), while the secondary outcomes were 3- and 5- year disease-free survival(DFS) and toxicity. P value(P), risk ratio(RR) and 95% confidence intervals(CIs) were calculated, and bias was analyzed. Results　Postoperative ChRT improved 3- and 5-year OS and 3- and 5- DFS compared to postoperative Ch(RR=0.737, 95%CI: 0.664～0.819, P=0.000; RR=0.818, 95%CI: 0.718～0.932, P=0.003; RR=0.770, 95%CI: 0.686～0.864, P=0.000; RR=0.772, 95%CI: 0.620～0.962, P=0.021), but also increased the digestive system toxicity and hematological system toxicity（RR=1.455, 95%CI: 1.134～1.868, P=0.003; RR=1.208, 95%CI: 1.036～1.410, P=0.016）. Conclusion　ChRT improves 3- and 5-year OS and 3- and 5-year DFS as compared to Ch alone, but also increases toxicities.]]> 2019/4/30 17:22:17 ZHANG Yan, SHEN Jian-Jun, SHEN Qi, et al. 141true ［Abstract］　Objective　To explore the diagnosis and prognostication values of multiple inflammatory factors in patients with sepsis. Methods　The white blood cell count(WBC), percentage of neutrophills(NEU%) and the levels of blood C-reactive protein(CRP), serum procalcitonin(PCT) and serum brain natriuretic peptide(BNP), and the APACHEⅡ scores in 235 septic patients and 251 non-septic patients were analyzed by the receiver operating characteristic(ROC), ANOVA, Jonckheere-Terpstra Test and U-test. Results　The ROC curve displayed that the AUC for PCT, CRP, and BNP was 0.792, 0.638, and 0.626, respectively. The sensitivity and specificity of these parameters were 82.90% and 52.30%(PCT), 80.50% and 31.20%(CRP), 86.60% and 35.20%(BNP), respectively. The results of ANOVA analysis showed that there were significant differences in PCT, CRP, BNP and APACHEⅡ among the infectious shock group, severe sepsis group, general sepsis group and non-sepsis group(P<0.01). Furthermore, the results of Jonckheere-Terpstra Test indicated that there were dose-effect relationships between PCT, CRP and BNP, with PCT having the most maximum J-T valuet(9.658), followed by CRP(3.734) and BNP(3.328), which suggested that PCT was the most valuable index in predicting the severity of infections. Compared the death and survival groups of sepsis, the results showed that there were signifcant differences in age and APACHEⅡ socres（P<0.05）, but there were no significant differences in gender, WBC, NEU%, CRP, PCT, and BNP between the two groups（P>0.05）. Conclusion　The levels of PCT, CRP and BNP are useful for the early diagnosis and evaluation of the severity of sepsis among which the value of PCT diagnosis and disease classification is optimal. Age and APACHEⅡ scores is the influencing factors for death.]]> 2019/4/30 17:22:17 LU Qiu-wei, LIAO Mei-juan, LIN Xue-zhen, et al. 140true ［Abstract］　Objective　To investigate the short-term and middle-term effects of adjustable autologous pericardial soft ring on treatment of functional tricuspid regurgitation(FTR). Methods　Seventy-two patients with FTR underwent tricuspid annuloplasty plus left heart valve surgery from December 2010 to December 2012, and their clinical data were retrospectively analyzed. Fresh autologous pericardial pericardium was used to make an adjustable pericardial soft ring for tricuspid annuloplasty in the autologous pericardial soft ring group(n=38), and De Vega suture tricuspid valvuloplasty was performed on the De Vega group(n=34). All the patients were followed up for 2 years after surgery. The tricuspid regurgitation(S_TR/S_TA), diastolic tricuspid annulus diameter(DTAD), right atrium diameter(RAD) and right ventricular inner diameter(RVD) were compared between the two groups before and 1 month, 2 years after surgery. Results　At 1 month after surgery, the parameters of DTAD, S_TR/S_TA, RAD and RVD were significantly less than those before surgery in the two groups(P<0.05). After a 2-year follow-up, DTAD in the De Vega group became significantly widened compared with that 1 month after surgery（P<0.05） while DTAD in the autologous pericardial soft ring group had no significant change（P>0.05）. After follow-up for 2 years, there were no significant differences in the parameters of S_TR/S_TA in the two groups compared with those 1 month after surgery（P>0.05）. However, the degree of reflux in the De Vega group increased significantly compared with that in the autologous pericardial soft ring group 2 years after surgery(P<0.05). Conclusion　The use of adjustable autologous pericardial soft ring is simple, safe and effective for the treatment of FTR, and its short-term and middle-term effects are significantly better than those of De Vega tricuspid annuloplasty.]]> 2019/4/1 16:03:53 ZHONG Yong-long, LONG Xiao-mao, HE Bang-fu, et al. 139true ［Abstract］　Objective　To investigate the clinical efficacy of hysteroscopic uterine curettage for cesarean section scar pregnancy(CSP) after pre-intervention of methotrexate(MTX) and mifepristone. Methods　The CSP patients hospitalized in our hospital from December 2016 to December 2017 were divided into two groups according to different treatment methods. The observation group(n=42) received hysteroscopic uterine curettage after treatment with MTX+mifepristone, and the control group(n=42) received hysteroscopic uterine curettage alone. The operation time, the amount of bleeding, the length of hospital stay, the time of blood β-HCG recovery to normal and the incidence of postoperative complications were compared between the two groups. Results　The intraoperative time, the time of blood β-HCG recovery to normal and the intraoperative blood loss in the observation group were significantly shorter or less than those in the control group(P<0.01). The length of hospital stay in the observation group was significantly longer than that in the control group(P<0.01). There was no significant differences in the incidence of postoperative complications between the two groups(P>0.05). Conclusion　Pre-intervention of MTX+mifepristone can shorten the operation time, reduce the amount of intraoperative blood loss in the treatment of CSP using hysteroscopic uterine curettage.]]> 2019/4/1 16:03:53 JI Meng-ge, MENG Yue-jin, GU Xiao-li, et al. 138true ［Abstract］　Objective　To explore the effect of Kangfuxin Liquid on cellular immunity in elderly patients with uterine prolapse receiving vaginal hysterectomy. Methods　Fifty elderly patients with uterine prolapse who received vaginal hysterectomy in the Department of Gynecology of the People′s Hospital of Guangxi Zhuang Autonomous Region during April 2014 and December 2017 were divided into study group and control group depending on whether voluntary oral admimistration of Kangfuxin Liquid was selected after surgery, with 25 cases in each group. The control group was given routine treatment such as antibiotics to prevent infections and carbazochrome to stop bleeding after vaginal hysterectomy, while the study group received the same treatment as the control group plus Kangfuxin Liquid on the second day after operation(10 ml per time, 3 times daily for 14 days). The changes in peripheral T-lymphocyte subsets（CD3⁺, CD4⁺, CD8⁺, CD4⁺/CD8⁺） were detected on day 2 after surgery(T₁) and day 16 after surgery(T₂), and the results were compared between the two groups. Results　There were no significant differences in the blood levels of CD3⁺, CD4⁺, CD8⁺ and CD4⁺/CD8⁺ T-lymphocyte subsets on day 2 after surgery(T₁) and day 16 after surgery(T₂) between the two groups(P>0.05). In the the study group, the blood level of CD4⁺ T cell on day 16 after surgery(T₂) was significantly higher than that on day 2 after surgery(T₁), while the serum level of CD8⁺ T cell was significantly lower than that on day 2 after surgery(T₁) （P<0.05）; there were no significant differences in the changes of CD3⁺ T cell and CD4⁺/CD8⁺ T cell(P>0.05). In the control group, there were no significant differences in the blood levels of CD3⁺, CD4⁺, CD8⁺ and CD4⁺/CD8⁺ T cells between T₁ and T₂(P>0.05). Conclusion　Adjuvant oral Kangfuxin Liquid therapy may better regulate cellular immune function in the elderly patiens with uterine prolapse who received vaginal hysterectomy. It is beneficial for the postoperative rehabilitation, however, it needs to be further verified by large sample and high-quality clinical researches.]]> 2019/4/1 16:03:53 WEI Li-ying, LI Xue, ZHAO Ren-feng, et al. 137true ［Abstract］　Objective　To study the clinical effect of expert type tibial intramedullary nailing on distal tibia fracture. Methods　The clinical data of 30 patients with distal tibia fractures receiving an expert intramedullary nail operation were retrospectively analyzed. The postoperative efficacy was summarized. Results　All the patients were followed up for 4～18 months(13.2 months in average). The clinical healing time of fractures was 4.2 months in average. Bone union was observed in all the patients. According to Joher-wruhs criteria, the excellent and good rate was 96.6%. Conclusion　The expert type tibial intramedullary nailing has a high healing rate in the treatment of distal tibia fractures with less trauma, fewer complications and better curative effect.]]> 2019/4/1 16:03:53 SHI Wei-ye, HUANG Jia-ji, WU Jiang-hua 136true ［Abstract］　Objective　To analyze the characteristics of anti-cE combined with anti-Mur causing difficulty in tests of blood typing and cross matching. Methods　Blood typing test was performed in two patients. The serum of the two patients reacted with reagent red blood cells whose blood groups were known in the phase of saline, papain, polyamine and antiglobulin, then the specificity of the antibodies was identified. Cross matching tests were performed by methods of saline phase, polyamine phase and micro gel column technique. Results　We found that anti-cE of IgM and IgG type and anti-Mur of IgM type in serum of case 1, anti-cE of IgG type and anti-Mur of IgM type in serum of case 2, caused difficulty in tests of blood group identification and cross matching. Conclusion　Anti-cE combined with anti-Mur may lead to difficulty in blood typing and matching for the patients with these two antibodies, and various methods should be used in the process of irregular antibody detection and cross matching, and the corresponding antigens of the blood provided to the patients should be confirmed as negative, to prevent hemolytic transfusion reaction caused by the results of false negative.]]> 2019/4/1 16:03:53 LI Jing, MO Zhu-ning, LI Hai-lan 135true ［Abstract］　Objective　To explore the feasibility of a new technique of artificial intelligence and image analysis based on the deep learning neural networks for cytological screening of cervical intraepithelial lesions. Methods　Three hundred and seventy-three cases who took physical examination in our hospital during July 2017 and December 2017 were diagnosed with cervical intraepithelial lesions by cervical biopsy under hysteroscopy and liquid-based cervical cytology. These patients were taken as the study subjects. The network liquid-based cytology results of the network intelligent image recognition system(the intelligent system) reading and manual reading were compared with the results of the colposcopy cervical tissue biopsy. Results　There was no significant difference between the two methods in sensitivity of cervical intraepithelial lesions(P>0.05). The specificity and coincidence rates of the intelligent system for cervical intraepithelial lesions were significantly lower than those of manual examination(P<0.05). Conclusion　The intelligent system may provide another practical and effective means of cytological screening of cervical intraepithelial lesions.]]> 2019/4/1 16:03:53 ZHANG Shi-hao, XIAN Li-ying, GAO Min, et al. 134true ［Abstract］　Objective　To explore the effect of atrial fibrillation(AF) on cognitive function in patients with chronic heart failure(CHF). Methods　One hundred and thirty-nine CHF patients were divided into AF group(64 cases) and non-AF group(75 cases). The difference of cognitive function was compared between the two groups. Results　The incidence of cognitive impairment was higher in CHF patients, reaching 79.86%. The incidence of cognitive impairment in the AF group(87.50%) was significantly higher than that in the non-AF group(73.30%), P<0.05. The cognitive function score of the CHF patients with AF was worse than that of CHF patients without AF(P<0.05). Conclusion　The incidence of cognitive impairment in CHF patients is higher, and the patients with AF are more likely to suffer from cognitive impairment.]]> 2019/4/1 16:03:53 CHEN Xiao, ZHANG Jia-wei, XU Dai-qiang, et al. 133true ［Abstract］　Objective　To observe the effects of rice wine on osteoblasts, osteoclasts and intraosseous pressure of rabbit femoral head. Methods　Sixty-four rabbits were randomly divided into experimental group and control group according to random number table method, with 32 cases in each group. The experimental group was given rice wine in a dose of 10 ml/(kg·d) by inserting stomach tube, 3 times a week. The control group was given the same amount of normal saline in the same way. Eight rabbits were randomly selected from the two groups respectively before and 1, 3, 6 months after experiment. The rabbits were sacrificed by air embolization after the internal pressure of the femoral head of left hind limbs was measured, and then the femoral head of right hind limbs was taken as specimens. The specimens underwent fixation, decalcification, dehydration, embedding and other steps, cutting to 5 μm, HE staining, and the number of osteoblasts and osteoclasts were calculated under light microscope. Results　There were no significant differences in the number of osteoblasts, osteoclasts and intraosseous pressure between the two groups before and one month after intragastric administration(P>0.05). The number of osteoblasts was significantly less, and the number of osteoclasts was significantly more, and the intraosseous pressure was significantly higher in the experimental group than those in the control group 3 and 6 months after intragastric administration(P<0.05). Conclusion　Long-term oral administration of rice wine (more than 3 months) can decrease the number of osteoblasts, increase the number of osteoclasts and raise the intraosseous pressure in the femoral head of rabbits.]]> 2019/3/4 16:47:08 HUANG Si-cheng, LI Hong-yu, XI Li-cheng, et al. 132true ［Abstract］　Objective　To observe the effects of benapril combined with calcitriol on renal function indices and microinflammatory factors in early diabetic nephropathy patients. Methods　Ninty-six early diabetie nephropathy patients were divided into control group and observation group, with 48 cases in each group. The control group was treated with benapril and the observation group was treated with benapril combined with calcitriol. The therapeutic effect, blood glucose, renal function indices, inflammatory factor level and adverse reaction were compared between the two groups. Results　There were no significant differences in blood glucose level and adverse reaction rate between the two groups(P>0.05). The renal function indices and inflammatory factor level of the observation group were significantly lower than those of the control group, and the total therapeutic efficiency of the observation group was significantly higher than that of the control group(P<0.05). Conclusion　Benapril combined with calcitriol can improve the therapeutic effect of patients with early diabetic nephropathy.]]> 2019/3/4 16:47:08 YANG Yu, YANG Hai-bo, HAN Lu, et al. 131true ［Abstract］　Objective　To compare the short-term curative effects of holmium laser enucleation of the prostate(HoLEP) vs plasmakinetic enucleation of the prostate(PKEP) in treatment of benign prostatic hyperplasia(BPH). Methods　Sixty cases of BPH were randomly divided into HoLEP group(treated with HoLEP) and PKEP group(treated with PKEP) by random number table method. The relevant data were collected during and after operation. IPSS,QOL,Qmax and PVR were recorded during the follow-up period. Results　There were significant differences between HoLEP group and PKEP group in the mean duration of operation［(47.6±7.1)min vs (74.2±9.7)min］, in bladder washing time［(9.3±2.1)h vs (16.7±4.3)h］, in the mean time of indwelling catheter［(72.8±5.1)h vs (79.6±5.4)h］, in the mean duration of hospitalization［(5.9±1.7)d vs (8.8±1.5)d］, and in the decrease of hemoglobin［(7.0±1.3)g/L vs (9.6±1.7)g/L］(P<0.05). There were no significant differences in the mass of the excised prostates and the incidence of complications between the two groups(P>0.05). There were no significant differences in IPSS, QOL, Qmax and PVR before and 6 months after operation between the two groups(P>0.05). Conclusion　Compared with PKEP group, HoLEP group has less bleeding, shorter operation time, bladder washing time and hospitalization time in the treatment of BPH. HoLEP may be the primary method of surgieal treatment for BPH.]]> 2019/3/4 16:47:08 PAN Jie, LIANG Jian-feng, CHEN Ju-feng, et al. 130true ［Abstract］　Objective　To analyze the clinical effects of Viabahn covered stents and bare metal stents in treatment of femoral and popliteal arteriosclerosis. Methods　 The clinical data of 75 patients(75 limbs) with femoral and popliteal arteriosclerosis obliterans receiving endovascular surgery from January 2016 to January 2017 were retrospectively analyzed. There were 35 patients in the Viabahn covered stents group and 40 in the bare metal stents group. The condition of vascular recanalization was compared between the two groups and the factors which affected the primary patency rate were also analyzed. Results　All the patients(75 limbs in 75 cases) were operated successfully. The follow-up time was 24 months. In the Viabahn covered stents group, the primary patency rates were 97.14%, 91.43%, 80.00% and 68.57% 3, 6, 12 and 24 months after surgery, and the secondary patency rates were 100.00%, 97.14%, 91.43% and 85.71% respectively. In the bare metal stents group, the primary patency rates were 90.00%, 75.00%, 52.50% and 42.50% 3, 6, 12 and 24 months after surgery, and the secondary patency rates was 97.50%, 92.50%, 87.50% and 80.00% respectively. Log-rank test showed that there were a significant differences in the primary patency rates between the two groups(χ²=5.318, P=0.021), but there were no significant difference in the secondary patency rates(χ²=0.536, P=0.464). Smoking, hyperlipidemia and diabetes were the independent risk factors of primary patency rates(P=0.007, 0.003, 0.063). Conclusion　Viabahn covered stent is more effective than bare metal stent in the treatment of femoral popliteal arteriosclerosis.]]> 2019/3/4 16:47:08 XU Heng, WANG Bing, WU Fei, et al. 129true ［Abstract］　Objective　To explore the clinical application value of double-balloon enteroscopy(DBE) in diagnosis and treatment of Meckel′s diverticulum(MD) bleeding in young patients. Methods　The clinical data of MD bleeding in 11 young patients who were diagnosed and treated with DBE in the hospital between July 2010 and April 2018 were retrospectively analyzed. Results　(1)The 11 patients came to the hospital for consultation because of repeated defecation of black stool or bloody stool. The small bowel bleeding or MD bleeding was considered by some examinations, while ileal diverticulum and bleeding lesions were found and MD bleeding was diagnosed by DBE. (2)One case of hemorrhagic diverticulitis was completed DBE microscopic hemostasis. One case of ileal artery hemorrhage underwent interventional therapy. Eleven patients eventually receiving laparoscopic surgery were improved and discharged. (3)DBE biopsy was performed in 6 cases and their diagnoses were consistent with the postoperative pathological results in 4 cases. (4)The patients were followed up untill April 2018. During the follow-up 2 cases were lost and 9 cases had no recurrent bleeding. Conclusion　(1)MD bleeding can be directly detected and the size and shape of MD can be observed by DBE, and MD bleeding can be treated with microscopic hemostasis. (2)The radical treatment for MD bleeding is surgery,especially laparoscopic surgery, which has a good long-term effect.]]> 2019/3/4 16:47:08 HUANG Shan-qun, LIU Xin, GUO Xian-wen, et al. 128true ［Abstract］　Objective　To analyze the causes of death of chronic non-communicable diseases in Guangxi national monitoring points from 2013 to 2017, and to provide the basic data for prevention and control of chronic diseases. Methods　The death data of chronic diseases were collected and sorted out in Guangxi national monitoring points from 2013 to 2017. The death of sequence of the chronic diseases, the constituent ratio of death, the characteristics of mortality rate and probability of early death caused by four main chronic diseases were analyzed. Results　Cerebrovascular diseases, malignant tumors, and heart disease were the main causes of deaths of chronic diseases in Guangxi national monitoring points. The mortality rate of chronic diseases was 545.82/100 000(The standardized mortality rate was 526.14/100 000), and the mortality rates of chronic diseases in male, female, urban residents and township residents were 643.29/100 000, 439.48/100 000, 474.10/100 000 and 578.18/100 000 respectively. The comparison of chronic diseases of the mortality rates among female residents in different years showed an upward trend(χ²=63.120, P=0.001), and urban residents also showed an upward trend(χ²=210.790, P=0.001), while township residents showed a downward trend(χ²=13.420, P=0.001). The mortality rate of chronic diseases of males was higher than that of females(χ²=8 874.550, P=0.001).The mortality rate of chronic diseases in rural residents was higher than that of urban residents(χ²=1 987.470, P=0.001). The mortality rate of chronic diseases increased with age. The leading causes of death in the population aged 0～4 years were congenital malformation and chromosomal abnormalities, but those of the population aged more than 65 years were cerebrovascular diseases and heart disease. From 2013 to 2017, the probabilities of early death caused by four main chronic diseases were 19.62%, 19.78%, 19.60%, 19.34% and 20.10% respectively. Conclusion　The changes in mortality rates of chronic diseases among women and urban residents in Guangxi national monitoring points show an upward trend in different years, and the township residents show a downward trend. The mortality rate of chronic diseases in male residents is higher than that in female residents, and the mortality rate of rural residents is higher than that of urban residents. The main causes of death are different in different ages. The probabilities of early death caused by four main chronic diseases are high.]]> 2019/3/4 16:47:08 HUANG Jin-mei, MAO Wei, CAI Jian-feng, et al. 127true ［Abstract］　Objective　To explore the application value of automated breast volume scanner(ABVS) in differential diagnosis of breast lesions. Methods　From March 2015 to January 2018 in our hospital, 103 breast tumor patients(130 lesions) were examined with two-dimensional ultrasound and ABVS inspections. The postoperative pathological results were taken as the gold standard to compare the diagnostic efficiency between the two examination methods. Results　Of the 130 lesions, 56 lesions were malignant, and 74 lesions were benign. The sensitivity, specificity and accuracy were 69.6%, 79.7%, 75.4% and 87.5%, 94.6%, 91.5% using two-dimensional ultrasound and ABVS, respectively. The occurrence rate(75.0%) of the convergence sign of ABVS in the malignant nodules was significantly higher than that in the benign nodules(5.4%), P<0.05. The detection rate(83.9%) of the malignant nodules(≤2 cm) was significantly higher than that of the malignant nodules(>2 cm)(61.5%), P<0.05. Conclusion　ABVS examination can significantly improve the accuracy of differential diagnosis of benign and malignant breast tumors. Especially, “convergence sign” can effectively improve the detection rate of small lesions of early breast cancer and therefore has high clinical application value.]]> 2019/3/4 16:47:08 XU Xi-ting, WEI Shu-jing, WEI Jun-yu, et al. 126true ［Abstract］　Objective　To compare the hemostatic effects and complications of three minimally invasive methods in treatment of acute upper gastrointestinal bleeding caused by portal hypertension, and to provide the reference for selection of suitable treatment methods for clinical practice. Methods　The clinical data of 73 patients with acute upper gastrointestinal hemorrhage caused by portal hypertension were collected in Jinhua Hospital of Zhejiang University between February 2013 and December 2017. These patients were divided into laparoscopic group(n=16), endoscopic group(n=31) and TIPS group(n=26) according to different treatment methods. The general situation, postoperative hospitalization time, postoperative complications, perioperative death and postoperative rehemorrhage were retrospectively analyzed and compared among the three groups. Results　The endoscopic group had the shortest average time of hospitalization（P<0.05） and the lowest hospitalization cost（P<0.05）, and the incidence of obdominal infection after operation in the laparoscopie group was the highest（P<0.05）. The incidence of hepatie encephalopathy after operation in the TIPS group was the hightest（P<0.05）. The laparoscopie group had the lowest rebleeding rate（P<0.05）. Conclusion　The three methods have their respective advantages in the treatment of acute upper gastrointestinal bleeding caused by portal hypertension, as a result, an appropriate treatment method should be selected based on the comprehensive consideration of the patient′s condition, the doctor′s experience and the technical conditions of the hospital.]]> 2019/1/31 16:31:41 BAI Yang, YU Shi-an, XU Long-tang, et al. 125true ［Abstract］　Objective　To observe the clinical effect of thyroxine replacement therapy on pregnant women with isolated hypothyroxemia. Methods　The prospective cohort study was performed in the pregnant women with isolated hyothyroidemia during the first and second trimester who were selected in our outpatients from March 2017 to March 2018. According to whether thyroxine replacement was used, the patients were divided into the intervention group(n=43) and the control group(n=48). The maternal and neonatal outcomes were analyzed. Results　There were no significant differences in delivery weeks, delivery methods and postpartum hemorrhage between the two groups(P>0.05). There were no significant differences in the incidence rates of hypertension, gestational diabetes, fetal growth restriction, premature rupture of fetal membranes and fetal distress between the two groups(P>0.05). There were no significant differences in neonatal birth weight and the rate of neonatal asphyxia between the two groups(P>0.05). Conclusion　Thyroxine replacement has no significant influences on pregnancy outcomes and perinatal events in pregnant women with isolated hypothyroxemia.]]> 2019/1/31 16:31:42 YANG Fang, WANG Su-mei, GONG Xiao-li 124true ［Abstract］　Objective　To investigate the effects of total parathyroidectomy with improved autotransplantation(TPTX+AT) of 5 autologous parathyroid tissue pieces(each one 1 mm×1 mm×1 mm) on secondary hyperparathyroidism(SHPT) to uremia. Methods　The patients with SHPT to uremia were selected from January 2016 to December 2017, and 5 autologous parathyroid tissue pieces(each one 1 mm×1 mm×1 mm) were transplanted into the patients′ healthy forearms after total parathyroid thyroidectomy. The serum levels of total parathyroid hormone(iPTH), serum calcium and phosphorus, relief of symptoms of SHPT, operative complications, postoperative hospitalization time and postoperative recurrence rate were observed. Results　Thirteen patients underwent this procedure(TPTX+AT). All the patients had no intraoperative laryngeal nerve injury. On the first day after operation, iPTH was reduced to normal level in all the patients. At 1 week, 3 months, 6 months and 12 months after operation, the serum iPTHs were significantly decreased compared with those before operation（P<0.05）. And all these postoperative indicators were within the normal ranges, with no recurrence cases. The symptoms of bone pain and pruritus were relieved or disappeared within one week, and the symptoms of bone deformation and height shortening were improved or did not progress. Conclusion　TPTX+AT is a safe, effective and better method for the treatment of SHPT to uremia. It can preserve the parathyroid function and control the postoperative recurrence of hyperparathyroidism.]]> 2019/1/31 16:31:42 LI Zong-kui, YAO Si-yang, LIANG Bin, et al. 123true ［Abstract］　Objective　To investigate the effect of continuous lumbar drainage on traumatic subarachnoid hemorrhage. Methods　Fifty patients with traumatic subarachnoid hemorrhage were divided the control group and observation group by random number table method, 25 cases in each group. The control group received lumbar puncture treatment, and the observation group was treated with continuous lumbar drainage. The parameters of cerebral blood volnine(CBV), cerebral blood flow(CBF), time to peak(TTP), mean transit time(MTT) in cerebral computed tomography(CT) perfusion imaging at different time points and the levels of endothelin-1(ET-1), nitric oxidex(NO) in the cerebrospinal fluid were compared between the two groups. Results　The parameters of CBV, CBF and NO in the observation group were obviously higher than those in the control group 7 and 14 days after treatment. The levels of TTP, MTT, ET-1 in the observation group were obviously lower than those in the control group 7 and 14 days after treatment（P=0.000）. Conclusion　Traumatic subarachnoid hemorrhage can induce cerebral vasospasm, and the continuous lumbar drainage can quickly remove the hemorrhagic cerebrospinal fluid to relieve the cerebral vasospasm, and improve cerebral blood perfusion and the prognosis of the patients.]]> 2019/1/31 16:31:42 ZHANG Chuan-dong, QIU Hong, LAN Zhan, et al. 122true ［Abstract］　Objective　To compare the effectivness and safety between NovaSure endometrial ablation and transcervical resection of endometrium(TRCE) in treatment of abnormal uterine bleeding(AUB). Methods　Sixty AUB patients whose previous medications were ineffective were selected from January 2015 to January 2017, and were randomly divided into study group(n=30) and control group(n=30). The study group was treated with NovaSure, and the control group was treated with TRCE. All the patients were followed up respectively at 1, 3, 6, and 12 months after operation to determine their response to the treatment and the incidence of complications. Results　The menstrual blood volume was significantly reduced in all the patients after operation, but there were no significant differences in effective rate and amenorrhea rate between the two groups(P>0.05). The operation time of the study group was significantly shorter than that of the control group（P<0.05）. The intraoperative blood loss of the study group was significantly less than that of the control group（P<0.05）. The intraoperative and postoperative complications of the study group were less than those of the control group, but there were only significant differences in electrolyte disturbance and abdominal pain 1 and 3 months after operation between the two group（P<0.05). Conclusion　Both NovaSure and TRCE are effective in treatment of AUB, but NovaSure treatment may be safer.]]> 2019/1/31 16:31:42 YI Wen-juan, CHEN Yu-jie, MI Si-qian, et al. 121true ［Abstract］　Objective　To explore the application value of the pronuclear morphology score and the presence of cytoplasmic halo in predicting the embryonic development potential and clinical pregnancy rate. Methods　In this retrospective study, 618 cases zygotes were assessed according to pronuclear scoring systems and checked for the formation of cytoplasmic halo. The embryo cleavage rate, day 3 frozen embryo rate, day 3 high-quality embryo rate, blastocyst formation rate, frozen blastocyst formation rate, high-quality blastocyst formation rate and the clinical pregnancy outcome were analyzed. Results　The day 3 embryo cleavage rate, high-quality embryo rate, blastocyst formation rate, and frozen blastocyst formation rate were significantly higher in Z1, Z2, Z3 zygotes than in Z4 zygotes(P<0.05). The blastocyst formation rate of Z1 was significantly higher than that of Z3(P<0.05). Compared with those in halo-negative zygotes, the embryonic development parameters were significantly higher than those in halo-positive zygotes(P<0.05). The embryonic development parameters of halo-positive Z1, Z2, Z3 zygotes were significantly higher than those of halo-negative Z1, Z2, Z3 zygotes(P<0.05), however, only the day 3 high-quality embryo rate was significantly higher in the halo-positive Z4 zygotes than in the halo-negative Z4 zygotes(P<0.05). Whether Day 3 embryos appeared or not, a halo at zygote stage was not significantly different in the clinical pregnancy rate and implantation rate(P>0.05). Conclusion　Pronuclear morphology score and the presence or absence of cytoplasmic halo can indicate the developmental potential of embryo in vitro, but the cytoplasmic halo can not predict the embryo implantation and clinical pregnancy rate effectively.]]> 2019/1/31 16:31:42 LI Zheng-da, XUE Lin-tao, TAN Wei-hong, et al. 120true ［Abstract］　Objective　To investigate the safety of stepwise administration in preoperative preparation for hyperthyroidism patients. Methods　Ninety patients with hyperthyroidism were randomly divided into control group(n=45) and observation group(n=45). The control group received conventional treatment and the observation group was treated with stepwise administration. The operation time, intraoperative blood loss and the rate of postoperative complications were compared between the two groups. Results　There were no significant differences between the two groups in the operation time, the amount of blood loss during operation and the incidence of postoperative complications(P>0.05). There were significant differences between the two groups in average hospitalization days and average hospitalization expenses（P<0.05）. Conclusion　Stepwise administration is as safe and reliable as the conventional treatment in the preoperative preparation for hyperthyroidism patients. It can shorten the hospitalization time and reduce the hospitalization expenses.]]> 2019/1/31 16:31:42 CHEN Shao-long, CHEN Yi-yu 119true ［Abstract］　Objective　To explore the expression changes of protein kinase C(PKC), protein kinase A(PKA), protein kinase G(PKG) and calcium/calmodulin-dependent protein kinase Ⅱ(CaMKⅡ) in cerebral astrocytes under oxygen and glucose deprivation. Methods　The cerebral cortex of 2-day-old newborn SD rats was purified and cultured for astrocytes, and the ischemic cell model was established by oxygen and glucose deprivation. The control group and the model group were grouped according to different experimental methods. Reverse transcription-polymerase chain reaction(RT-PCR) was used to detect the expressions of aquaporin-4(AQP4), and Western blot was used to detect the expressions of PKC, PKA, PKG and CaMKⅡ in astrocytes. The differences in the indicators were compared between the two groups. Results　The expression of AQP4 in the model group was significantly higher than that in the control group(P<0.05). The expression of PKC in the model group was significantly lower than that in the control group(P<0.05). The expression of CaMKⅡ in the model group was significantly higher than that in the control group(P<0.05). The expressions of PKA and PKG were not significantly different between the two groups(P>0.05). Conclusion　Decreased PKC expression and increased CaMKⅡ expression in astrocytes caused by oxygen and glucose deprivation may be related to edema of astrocytes.]]> 2019/12/30 10:12:41 FAN Bing-lin, WEI Jun-jie, LI Yan-hua, et al. 118true ［Abstract］　Objective　To explore the correlation between polymorphism of 5,10-methylenetetrahydrofolate reductase(MTHFR) gene and the occurrence of acute coronary syndrome(ACS) in Han population in the central area of Henan province. Methods　Two hundred and eighty ACS patients of Han population in the central area of Henan province were recruited as the observation group, and 286 healthy subjects of Han population in the central area of Henan province were selected as the control group. The genotypes of C677T and A1298C sites of MTHFR gene were detected by fluorescence staining in situ hybridization. The differences of genotypes and allele distribution were compared between the two groups. The correlation between MTHFR gene polymorphism and ACS was investigated by binary Logistic regression analysis. Results　The distribution frequencies of genotypes in both groups were all in line with Hardy-Weinberg equilibrium(P>0.05). In the control group, the distribution frequencies of CC, CT and TT genotypes in MTHFR C677T site were 31.82%, 47.90% and 20.28%, respectively; the distribution frequencies of AA, AC and CC genotypes in MTHFR A1298C site were 73.78%, 21.68% and 4.54%, respectively. In the observation group, the distribution frequencies of CC, CT and TT genotypes in MTHFR C677T site were 16.43%, 40.71% and 42.86%, respectively; the distribution frequencies of AA, AC and CC genotypes in MTHFR A1298C site were 69.29%, 27.14% and 3.57%, respectively. There were significant differences in genotype frequencies and allele frequency of MTHFR C677T between the two groups(P<0.05), while there were no significant differences in genotype frequencies and allele frequency of MTHFR A1298C between the two groups(P>0.05). Binary Logistic regression analysis showed that MTHFR C677T genotype was the influencing factor of ACS(P<0.05). With TT genotype as the reference, the possibility of ACS in CC genotype was 24.4% of TT genotype, and that in CT genotype was 40.2% of TT genotype. Conclusion　MTHFR gene polymorphism is related to the occurrence of ACS in the Han population in the central area of Henan province, among which C677T mutation may be the influencing factor of ACS, while A1298C gene polymorphism may be low related to the occurrence of ACS.]]> 2019/12/30 10:12:41 ZHANG Yun-fei, XU Bing-xin, WU Fan, et al. 117true ［Abstract］　Objective　To investigate the clinical effects of trimetazidine combined with L-carnitine on treatment of uremic cardiomyopathy. Methods　Fifty-eight patients with uremic cardiomyopathy were selected in our hospital during January 2016 and December 2017 and were randomly divided into control group and observation group. The control group(n=29) was treated with L-carnitine and routine comprehensive treatment, and the observation group(n=29) was treated with trimetazidine combined with L-carnitine and routine comprehensive treatment. Both of the two groups were treated continuously for 3 months. The changes of heart function were compared between the two groups before and after treatment. Results　After treatment, the levels of brain natriuretic peptide(BNP), creatine kinase(CK), creatine kinase isoenzyme(CK-MB) and lactate dehydrogenase(LDH) in the two groups were significantly lower than those before treatment, and the decreased effects of the observation group were more significant than those of the control group(P<0.05). After treatment, the levels of left ventrcular ejection fraction(LVEF) in the two groups were significantly higher than those before treatment, and the increased effect of the observation group was more significant than that of the control group(P<0.05). Conclusion　Trimetazidine combined with L-carnitine is more effective than L-carnitine alone in treatment of uremic cardiomyopathy. Trimetazidine combined with L-carnitine therapy can reduce myocardial ischemia, improve myocardial contractility and cardiac function of the patients. This combination therapy is convenient, safe and has few side effects, which is worthy of clinical application.]]> 2019/12/30 10:12:41 XIE Ying, ZHA Yan, LIN Xin, et al. 116true ［Abstract］　Objective　To explore the diagnostic value of fibrinogen to albumin ratio(FAR) for no-reflow of coronary artery after emergency percutaneous coronary intervention(PCI) in patients with acute ST-segment elevation myocardial infarction(STEMI). Methods　The clinical data of 218 patients who were diagnosed with STEMI and treated with emergency PCI within 12 hours in our hospital from December 2016 to December 2018 were retrospectively analyzed. The patients were divided into reflow of coronary artery group(n=168) and no-reflow of coronary artery group(n=50) according to whether there was reflow of coronary artery after PCI. The clinical baseline data and medical laboratory results were compared between the two groups. Multivariate Logistic regression analysis was used to screen the influencing factors of no-reflow coronary artery. Receiver operating characteristic(ROC) curve was used to evaluate the value of FAR in diagnosis of no-reflow coronary artery. Results　The levels of left ventricular ejection fraction(LVEF) and albumin in the no-reflow of coronary artery group were significantly lower than those in the reflow of coronary artery group, while the levels of fibrinogen, high-sensitivity C-reactive protein(hs-CRP) and FAR in the no-reflow of coronary artery group were significantly higher than those in the reflow of coronary artery group(P<0.05). Multivariate Logistic regression analysis showed that higher levels of FAR and fibrinogen were the risk factors of no-reflow of coronary artery, while higher level of albumin was the inhibiting factor. The ROC curve showed that the area under the curve of FAR in diagnosing no-reflow of coronary artery was 0.750, and the diagnostic threshold was 10.82. The sensitivity and specificity of the diagnostic threshold were 83.3% and 64.0%, respectively, which had diagnostic value on no-reflow of coronary artery in patients with acute STEMI(P<0.05). Conclusion　FAR is the influencing factor of no-reflow coronary artery in STEMI patients after PCI, and it has certain value in the diagnosis of no-reflow coronary artery.]]> 2019/12/30 10:12:41 WANG Shun-li, TANG Jian-min, WANG Feng-yun, et al. 115true ［Abstract］　Objective　To explore the characteristics of ultrasonography and contrast-enhanced ultrasonography(CEUS) in non-lactating women with mastitis masses. Methods　The imaging data of 52 cases of mastitis masses of non-lactating patients receiving ultrasonography and CEUS for breast masses were collected in our hospital from January 2008 to December 2016. The charactristics of ultrasonography and CEUS were analyzed. Results　In the 52 mastitis masses of the non-lactating women, their high frequency color ultrasound images showed irregular substantial low mixed echo located in a shallower position with unclear boundary of the skin, and the thickened surface skin edema or blurred layers. The masses of often have similar manifestations to malignant tumors. The characteristics of CEUS showed heterogeneous high enhancement, and the size of masses at CEUS examination appeared larger than that at conventional two-dimensional utrasonographic examination with enhancerment of larger striated vessels around and inside. The most characteristics of the CEUS showed a delimited state because of the entrance of high dose contrast agent with single and multiple round or oval areas without enhancement. The accuracy of high frequency color ultrasonography was 57.69%(30/52), and the accuracy of high frequency color ultrasonography combined with contrast-enhanced ultrasonography was 82.69%(43/52), US-BI-RADS was classified as Ⅲ～Ⅴ. Conclusion　Ultrasonography and CEUS show some distinctive manifestations in mastitis masses and combination of the two examinations is helpful to reduce the rate of misdiagnosis and missed diagnosis, and increase the diagnostic coincidence rate.]]> 2019/12/30 10:12:41 HE Yan, WANG Xiao-yan, HU Qiao, et al. 114true ［Abstract］　Objective　To investigate hearing improvement in the patients with chronic suppurative otitis media after intact canal wall mastoidectomy plus tympanoplasty, and to provide reference for clinical treatment. Methods　Ninety-two cases of chronic suppurative otitis media receiving intact canal wall mastoidectomy plus tympanoplasty were selected in our hospital from May 2015 to May 2018. All the patients were given pure tone audiometry, acoustic impedance and temporal bone CT examination before operation. Pure tone audiometry was performed 3 months after operation. The results of pure tone audiometry were used as the evaluation index of hearing function. Results　Compared with those before operation, the patients′ postoperative air conduction threshold decreased significantly, and the bone conduction threshold increased significantly, and the reduction of air bone conduction decreased significantly(P<0.05). At the low frequency band(0.5～2.0 kHz), there were no significant differences between the preoperative and the postoperative bone conduction thresholds(P>0.05). At the high frequency band(4.0～8.0 kHz), the auditory threshold of bone conduction after operation was significantly higher than that before operation(P<0.05). Hearing improvement was markedly effective in 75 cases(81.52%), effective in 12 cases(13.04%) and ineffective in 5 cases(5.44%), and the total effective rate was 94.56%. Conclusion　Intact canal wall mastoidectomy plus tympanoplasty has significant improvement effect on hearing in patients with chronic suppurative otitis media, but drill noise can cause hearing loss in high frequency area of bone conduction.]]> 2019/12/30 10:12:41 HUO Dong-dong, LI Wei, LI Yuan-ye 113true ［Abstract］　Objective　To study the levels and distributions of hemoglobin A2(Hb A2) and hemoglobin F(Hb F) in hemoglobin E(Hb E) disease. Methods　One hundred and seventy samples from the Hb E disease patients were selected and were divided into Hb E heterozygotes group, Hb E homozygotes group, Hb E with α-thalassemia group and Hb E with β-thalassemia group according to the thalassemia gene analysis results. The levels of Hb A2 and Hb F and their distributions in each group were analyzed by capillary electrophoresis. Results　The results showed 125 cases in the Hb E heterozygotes group, 5 cases in the Hb E homozygotes group, 21 cases in the Hb E with α-thalassemia group, and 19 cases in the Hb E with β-thalassemia group. The average levels of Hb A2 in the four groups were (3.66±0.38)%, (9.64±0.76)%, (3.70±0.37)% and (5.83±1.49)%, respectively. The level of Hb F was (5.22±3.90)% in the Hb E homozygotes group, and (36.81±13.97)% in the Hb E with β-thalassemia group. In the Hb E homozygotes group and the Hb E with β-thalassemia group, the Hb A2 levels were mainly in the high value region(Hb A2≥4.0%), and in the other two groups the Hb A2 levels were mostly in the low value(2.5%Conclusion　In Hb E disease, the levels and distribution frequencies of Hb A2 and Hb F vary with genotypes, and generally increase with the severity of the disease.]]> 2019/12/30 10:12:41 SU Heng-xue, LIANG Liang, LI You-qiong, et al. 112true ［Abstract］　Objective　To investigate the effects of pelvic floor therapeutic apparatus combined with recombinant human granulocyte-colony stimulating factor(G-CSF) for intrauterine perfusion on improving endometrial receptivity. Methods　Sixty patients wtih thin endometria were selected from the Reproductive Medicine Centre of the People′s Hospital of Guigang City for in-vitro fertilization(IVF) assisted pregnancy during February 2017 and July 2018. All the patients were prepared for artificial cycles and then underwent frozen-thawed embryo transfer(FET). According to the visiting order, the patients were divided into the control group(treated with G-CSF for intrauterine perfusion) and the observation group(treated with G-CSF for intrauterine perfusion combined with pelvic floor therapeutic apparatus), with 30 cases in each group. The endometrial thickness(Em), resistance index(RI) and the proportion of type A endometria were compared between the two groups before and after treatment. The embryo implantation rate and the pregnancy rate were compared between the two groups after treatment. Results　After treatment, the Em value and the type A endometrium rate of the observation group were significantly greater than those of the control group, and the RI value of the observation group was significantly less than that of the control group(P<0.05). There were no significant differences in the embryo implantation rate and the pregnancy rate between the two groups after treatment(P>0.05). Conclusion　The pelvic floor therapeutic apparatus combined with G-CSF for intrauterine perfusion can improve the endometrial receptivity of the thin endometria and increase the Em.]]> 2019/12/30 10:12:41 HUANG Wei-mei, CHEN Chang-yi 111true ［Abstract］　Objective　To investigate and evaluate the healthy lifestyles of Zhuang nationality residents in Wuming District of Nanning City, and provide data support for relevant departments to formulate targeted strategies for chronic disease prevention and control. Methods　A questionnaire survey was conducted among 500 permanent Zhuang residents in Wuming District of Nanning City by stratified random sampling to understand the current status and characteristics of their healthy lifestyles. Results　The awareness rate of balanced diet among the Zhuang residents was 87.0%, and the awareness rate of recommended intake of edible salt and cooking oil was less than 40.0%. Only 25.0% of the Zhuang residents knew the normal value of BMI, and the awareness rate of the recommended level of physical activity was only 58.8%, 71.2% and 92.0% of the residents could avoid smoking and excessive drinking. The utilization rates of all kinds of health supporting tools were less than 10.0%, and the self-conscious oil and salt control and weight control were not good. Conclusion　The awareness rate of healthy lifestyle knowledge and the holding rate of healthy behavior of Zhuang nationality residents in Wuming District of Nanning City are relatively low. Health education and health guidance for Zhuang residents should be strengthened.]]> 2019/12/30 10:12:41 HUANG Jian-shuang, CHEN Hai-bin, CHEN Yan-ping, et al. 110true ［Abstract］　Objective　To investigate the influences of dietary habits and various elements of metabolic syndrome on hyperuricemia in Jinan area, and to provide scientific guidance for prevention and control of hyperuricemia. Methods　A total of 200 permanent residents of Jinan city were selected as the research subjects at the Health Management Center of the hospital from February 2017 to October 2018. Among them, 100 cases of hyperuricemia were taken as case group, and 100 cases of healthy persons receiving physical examination as control group. The basic information, dietary habits, physical measurements and biochemical indicators of the subjects were collected and statistical analysis was conducted. Results　Multiple linear regression analysis showed that body mass index(BMI), total cholesterol(TC), triglyceride(TG), low-density lipoprotein cholesterol(LDL-C) and creatinine(Cre) were the positive influencing factors of serum uric acid level(P<0.05), while high-density lipoprotein cholesterol(HDL-C) was the negative influencing factor(P<0.05). Binary Logistic regression analysis showed that smoking history, regular beer consumption, frequent consumption of animal internal organs, and frequent consumption of sweet drinks were the risk factors of hyperuricemia, while regular supplement of vitamin C and regular consumption of coarse grains were the inhibition factors of hyperuricemia(P<0.05). Conclusion　High BMI and hyperlipidemia are correlated with high uric acid level. Reducing alcohol consumption, eating less animal organs and other foods rich in purine, adding regular vitamin C supplementation and eating more coarse grains can prevent or reduce the occurrence of hyperuricemia.]]> 2019/11/28 12:01:08 ZHANG Jie, CAO Xiao-wei, ZHANG Xin, et al. 109true ［Abstract］　Objective　To investigate the effects of bloodstream infection of Staphylococcus aureus on platelet(PLT) counts in peripheral blood. Methods　Three hundred and twenty-eight cases of Staphylococcus aureus infection in our hospital from January 2014 to December 2016 were selected as the study subjects, including 81 cases of bloodstream infection(SAB), 103 cases of respiratory infection(SAR), 94 cases of wound local infection(SAW) and 50 cases of urinary tract infection(SAU). The changes of PLT and mean platelet volume(MPV) under different infection conditions were analyzed, and the correlations of other clinical and laboratory parameters with different PLT changes after infection control in the SAB group were investigated. Results　After infection control, the levels of PLT in the SAB group, the SAR group and the SAW group were significantly higher and the levels of MPV were significantly lower than that those before infection control(P<0.05). There were no significant differences in the levels of PLT and MPV before and after control of Staphylococcus aureus infection in the SAU group(P>0.05). After infection control, the PLT changes in the SAB group were significantly correlated with the time needed for infection control and the change rate of MPV(P<0.05). The results of correlation analysis showed that there was a negative correlation between PLT change rate and MPV change rate after infection control(P<0.05), and a positive correlation between PLT change rate and infection control time(P<0.05). Conclusion　Bloodstream infection of Staphylococcus aureus can lead to a decrease in the number of peripheral blood PLT in patients, and the change rate of PLT after infection control is negatively correlated with MPV change rate, which is related to the activation and aggregation of PLT.]]> 2019/11/28 12:01:08 CHEN Ya-wen, GU Jun-juan, LI Yi-rong 108true ［Abstract］　Objective　To analyze the clinical manifestations and risk factors of liver cirrhosis complicated with portal vein thrombosis(PVT). Methods　Fifty-nine patients with liver cirrhosis complicated with PVT were selected as PVT group from the Second Affiliated Hospital of Zhengzhou University during October 2012 and October 2018, and 78 cases of liver cirrhosis without PVT in the same period were randomly selected as controls(non-PVT group). The general clinical data, laboratory examination, imaging examination, clinical manifestations and complications of the patients were collected. The clinical features were compared between the two groups and the influencing factors of PVT were analyzed. Results　There were significant differences in platelet count, mean platelet volume, platelet distribution width, D-dimer, fibrinogen, inner diameter of the main portal vein, spleen thickness, spleen length, inner diameter of the spleen vein, the history of esophageal ligation and injection of gastric fundus sclerosing agent, and the history of splenectomy between the two groups(P<0.05). The results of binary Logistic regression analysis showed that increased platelet count(OR=1.014, P=0.008), widened inner diameter of the splenic vein (OR=1.959, P=0.000), increased thickness of spleen(OR=1.117, P=0.012), and history of splenectomy(OR=4.559, P=0.001) were the independent risk factors of PVT in the patients with liver cirrhosis. Receiver operating characteristic(ROC) curve analysis showed that the area under the curve(AUC) of the spleen thickness, the platelet count and inner diameter of splenic vein were 0.595, 0.640 and 0.725 respectively in the diagnosis of liver cirrhosis complicated with PVT, and the index of the inner diameter of the splenic vein had the highest diagnostic value. The incidence rates of abdominal distention, gastrointestinal hemorrhage, fever, ascites and hepatic encephalopathy had no significant differences(P>0.05), while the incidence of abdominal pain had significant difference between the two groups(P<0.05). Conclusion　Enlargement of the index of the inner diameter of the splenic vein diameter, increase of platelet count, thickening of spleen and history of splenectomy are the independent risk factors of liver cirrhosis complicated with PVT in patients with cirrhosis. PVT can aggravate the clinical symptoms and increase the incidence of related complications in patients with liver cirrhosis.]]> 2019/11/28 12:01:08 YANG Jiao-jiao, LU Gao-feng, WU Jia-xun, et al. 107true ［Abstract］　Objective　To study the relationship between helicobacter pylori(Hp) infection and cholecystolithiasis, cholecystitis and gallbladder polyps, and to explore the clinical value of Hp eradication in prevention of gallbladder diseases. Methods　Nine hundred and sixty-two cases with abdominal discomfort and detection of Hp urease antibody were collected in our hospital from June 2018 to December 2018 and their clinical data were retrospectively analyzed. The relationship between the occurrence of cholecystitis, cholecystolithiasis and gallbladder polyps and Hp infection, and the clinical characteristics of gallbladder diseases under different Hp infections were analyzed. Results　In this study, there were 433 cases in the Hp positive group and 529 cases in the Hp negative group, and the Hp infection rate was 45.01%(433/962). The average age of the patients and the proportion of the patients with cholecystolithiasis and cholecystitis in the Hp positive group were significantly larger than those in the Hp negative group(P<0.05). In the patients with cholecystolithiasis, the total cholesterol level of the Hp positive patients was significantly higher than that of the Hp negative patients(P<0.05). In the patients with cholecystitis, the average white blood cell count of the Hp positive patients was significantly higher than that of the Hp negative patients(P<0.05). Conclusion　Hp infection may be associated with the occurrence and development of cholecystitis and cholecystolithiasis.]]> 2019/11/28 12:01:08 ZHANG Ling, ZHANG Ye, ZHANG Xue, et al. 106true ［Abstract］　Objective　To detect the levels of fecal bile acid spectrum in patients with hepatitis-B-related acute-on-chronic liver failure(ACLF), and to understand its significance in the development of the disease. Methods　Twenty patients diagnosed with ACLF were selected as the observation group, and fourteen healthy individuals were randomly selected as the control group. The levels of fecal bile acid(cholic acid, lithocholic acid, deoxycholic acid,chenodeoxycholic acid,ursodeoxycholic acid and total bile acid) were detected by high performance liquid chromatography(HPLC). Results　The level of fecal cholic acid in the observation group was significantly higher than that in the control group(P<0.05). There were no significant differences in the levels of lithocholic acid, deoxycholic acid, chenodeoxycholic acid, ursodeoxycholic acid and total bile acid between the observation group and the control group(P>0.05). There were no significant differences in the levels of bile acid between the patients with hepatic encephalopathy and those without hepatic encephalopathy(P>0.05). There were no significant differences in the levels of bile acid between the patients with ascites and those without ascites(P>0.05). There were no significant differences in the levels of bile acid between the patients with Child-Pugh B and those with Child-Pugh C patients(P>0.05). Conclusion　The levels of fecal cholic acid of ACLF patients are higher than those of healthy individuals, but there are no significant differences in total bile acid and other bile acids between ACLF patients and healthy individuals.]]> 2019/11/28 12:01:08 MENG Dan-li, LIANG Lie-xin, SONG Huai-yu 105true ［Abstract］　Objective　To explore the correlation between six-minute walk test and lung function and blood gas analysis in pneumoconiosis patients. Methods　One hundred and ten pneumoconiosis patients with definite clinical diagnosis in our hospital were selected for six-minute walk test, and static pulmonary function and arterial blood gas analysis indexes were detected. Results　The six-minute walk distance(6MWD) was positively correlated with FVC%, FVE1%, FEV1/FVC and PaO₂. Conclusion　Six-minute walk test can evaluate the pulmonary function and systemic functional status of pneumoconiosis patients. It is cheap, easy to operate and worthy of promotion.]]> 2019/11/28 12:01:08 TANG Zhong-quan, DENG Ting-ting 104true ［Abstract］　Objective　To observe the curative effect of transcutaneous electric nervous stimulation(TENS) on pain relief after hysteroscopy. Methods　Four hundred and fifty patients who underwent hysteroscopic surgery in the Department of Gynecology, the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2017 to July 2018 were selected as the research subjects. The research subjects were randomly divided into TENS treatment group, drug treatment group and placebo control group, with 150 cases in each group. The changes of pain scores before and 30, 60, 120 min after treatment were compared by Visual Analogue Scale(VAS) scoring method among the three groups. Results　There were no significant differences in the VAS scores before treatment among the three groups(P>0.05). Compared with those before treatment, the VAS scores of the TENS treatment group were significantly decreased after 30 minutes of treatment(P<0.05). There were no significant changes in VAS scores before and after treatment in the drug treatment group and the placebo control group. Compared with those before treatment, the VAS scores were significantly decreased after 60 minutes of treatment in the three groups(P<0.05), and the reductions of VAS scores in the TENS treatment group and the drug treatment group were more obvious than those in the placebo control group(P<0.05). After 120 minutes of treatment, the VAS scores of the three groups continued to decline, and the differences before and after treatment were statistically significant(P<0.05). However, there were no significant differences in VAS scores among the three groups(P>0.05). Conclusion　TENS is safe and effective in pain management and may be a main method to relief pain after hysteroscopy.]]> 2019/11/28 12:01:08 XU Li-li, YANG Liu, ZHAO Ren-feng 103true ［Abstract］　Objective　To explore the feasibility of serum pepsinogen(PG) in predicting the efficacy of proton pump inhibitors(PPIs) in patients with functional dyspepsia(FD). Methods　One hundred patients with FD were selected in our hospital from March 2016 to March 2019. All the patients were treated with rabeprazole(20 mg, qd), and before the treatment, the serum of all the patients was taken for prostaglandin Ⅰ(PGⅠ) and prostaglandin Ⅱ(PGⅡ) detections. After 4 weeks of treatment, the research subjects were divided into the effective group(n=54) and the ineffective group(n=46) according to the Gastrointestinal Symptom Rating Scale(GSRS). The levels of PGⅠ, PGⅡ and PGR(PGⅠ/PGⅡ) were compared between the two groups before treatment. Spearman rank correlation analysis was used to analyze the correlations of PGⅠ, PGⅡand PGR with the therapeutic effect of PPIs. Results　The levels of serum PGⅠ and PGR in the effective group were significantly higher than those in the ineffective group before treatment(P<0.05). The level of PGⅡ in the effective group was lower than that in the ineffective group, but with no significant difference(P>0.05). The abnormal rate of serum PGR in the ineffective group was significantly higher than that in the effective group before treatment(P<0.05). The results of Spearman rank correlation analysis showed that PGⅠ and PGR were positively correlated with the reduction of symptoms scores after treatment(P<0.05). Conclusion　The PG level of FD patients before treatment has clinical guiding value in predicting the efficacy of PPIs in FD patients.]]> 2019/11/28 12:01:08 NING Lin, WEI Liang-hong, CHEN Hai-dong, et al. 102true ［Abstract］　Objective　To evaluate the early predictive value of amplitude integrated electroencephalogram(aEEG) for brain injury in premature infants. Methods　 Ninety-one preterm infants with high risk factors of brain injury were divided into the brain injury group(n=47) and the non-brain injury group(n=44) according to bedside color B-ultrasound and neurological symptoms. aEEG was performed within 6 hours after birth, and magnetic resonance imaging(MRI) was performed 10～14 days after birth. Children′s Development Center of China(CDCC) infant intelligence development scale examination was performed after 3 months of corrected gestational age. According to the background activity, amplitude and sleep wake cycle(SWC) of aEEG, the abnormal degrees of aEEG which were classified as normal degree, mildly abnormal degree and severely abnormal degree were judged. The relationships between the different classifications of aEEG and CDCC scale and MRI were analyzed. Results　In the mildly abnormal aEEG group(45 cases), the MRI positive rate was 37.78%; the psychomotor development index(PDI) scores of CDCC corrected for 3 months were (92.5±10.6)points, and the mental development index(MDI) scores were (95.7±12.4)points. In the severely abnormal aEEG group(19 cases), the MRI positive rate was 73.68%; the PDI scores were (66.3±17.4)points, and the MDI scores were (67.2±15.1)points. There were significant differences in MRI positive rates, PDI scores and MDI scores between the two groups(P<0.05). Conclusion　aEEG has diagnostic value for brain injury in premature infants, and early intervention can reduce the brain injury, which is worthy of clinical promotion and application.]]> 2019/11/28 12:01:08 SHI Xue-kai, NONG Chang-liang, HE Xiao, et al. 101true ［Abstract］　Objective　To summarize the clinical experience of minimally invasive aortic valve replacement through right parasternal longitudinal small incision, and to evaluate its safety and curative effect. Methods　From December 2014 to March 2019, 19 patients including 10 males and 9 females with aortic valve disease underwent minimally invasive aortic valve replacement through right parasternal longitudinal small incision in Cardiac Surgery Center of Sichuan Provincial People′s Hospital. Cardiopulmonary bypass was established for all the patients through femoral artery and vein. The vacuum assisted venous drainage technology was used for enhancing the venous drainage. The left ventricular drainage cannula was inserted to the left ventricle through the right superior pulmonary vein. Surgical resection of the diseased aortic valve was performed under direct vision, and interrupted suture was used for the aortic valve replacement. An artificial valve was implanted for the aortic valve replacement. Results　All the patients underwent surgery successfully, and none of them underwent enlarged incision or anterior median thoracotomy. The operation time was (256.05±47.54)minutes, and the cardiopulmonary bypass time was (110.79±23.01)minutes. The aortic cross-clamping time was (71.37±14.93)minutes. The volume of blood loss during operation was (231.58±129.33)milliliters. The transfusion volume of autoblood after operation was (314.21 ±77.84)milliliters. The transfusion volume of bank blood was (257.89 ±238.20)milliliters, and there were 8 patients(42.11%) without bank blood transfusion. The postoperative ventilator support time was (7.95±2.57)hours. The postoperative intensive care unit stay time was (29.16±10.76)hours. The postoperative drainage tube placement time was (3.47±0.77)days. The length of incision was (4.93±0.57)centimeters. Poor healing of incision was not found in all the patients. Bleeding in the aortic and pulmonary artery space after stopping cardiopulmonary bypass was found in 1 case whose direct repair was difficult and the repair was successful after direct repair of the heart and again parallel circulation emptying. Right lower pulmonary atelectasis occurred in 1 case after operation and lung recruitment was achieved after treatment. All the patients were cured and discharged from hospital. The postoperative hospital stay time was (8.58±2.24)days. The patients were followed up for (25.68±20.07)months after operation, and the quality of life was good, and their heart function recovered to grade Ⅰ. Color Doppler ultrasound showed no obvious abnormalities of the artificial valves. The left ventricular ejection fraction(LVEF) and left ventricular end diastolic diameter(LVEDD) were significantly improved compared with those before the operation(P<0.05). Conclusion　Minimally invasive aortic valve replacement through right parasternal longitudinal small incision with peripheral cardiopulmonary bypass established through femoral artery and vein is less invasive, and has fewer complications, faster recovery and better effect. It is worthy to be applied in experienced cardiac surgery centers.]]> 2019/10/30 10:56:24 LIU Sheng-zhong, WEI Da-chuang, TAN Jin, et al. 100true ［Abstract］　Objective　To compare the clinical effects of different doses of atorvastatin on treatment of non-ST segment elevation acute coronary syndromes(NSTE-ACS). Methods　A total of 41 patients with NSTE-ACS who were hospitalized from January 2017 to December 2018 were divided into treatment group(n=21) and control group(n=20) by random number table method. The patients in both groups received routine treatment. The treatment group received atorvastatin 20 mg in addition to the routine treatment. The control group received atorvastatin 80 mg orally on the first day after routine treatment, followed by atorvastatin 40 mg per day. The course of treatment in both groups was 6 months. The changes of total cholesterol(TC), low density lipoprotein cholesterol(LDL-C) and triglyceride(TG) and the occurrence of cardiovascular events were compared between the two groups before and after treatment. Results　The levels of serum TC, TG and LDL-C in the two groups 2, 4 and 24 weeks after treatment were significantly lower than those before treatment(P<0.05). The levels of serum TC and LDL-C in the control group were significantly lower than those in the treatment group 2 and 4 weeks after treatment, and the levels of TG were significantly higher than those in the treatment group(P<0.05). There were no significant differences in the levels of serum TC, LDL-C and TG and the incidence of adverse cardiovascular events between the treatment group and the control group 24 weeks after treatment(P>0. 05). Conclusion　Moderate intensity atorvastatin(20 mg/d) is similar to high intensity atorvastatin(40 mg/d) on reducing the blood lipid level and the occurrence of adverse cardiovascular events in NSTE-ACS patients.]]> 2019/10/30 10:56:24 LIU Yong, LIU Yan-li, MO Song-liu, et al. 99true ［Abstract］　Objective　To explore the expression of survivin in pancreatic head carcinoma tissues and its clinical characteristics. Methods　The expression of survivin was detected in 38 cases of surgically resected primary pancreatic head carcinoma tissues by immunohistochemical method, and its relationship with clinicopathological parameters and postoperative survival rate were analyzed. Results　The positive expression rate of survivin was 63.16% in the 38 cases of pancreatic head carcinoma. The expression of survivin was significantly correlated with tumor differentiation(P<0.05). Survival analysis showed that the survival time of the pancreatic head carcinoma patients with positive expression of survivin was shorter than that of the pancreatic head carcinoma patients with negative expression of survivin(P<0.05). Cox regression analysis showed that higher TNM stage, lymph node metastasis, lower differentiation of the tumor and survivin positive expression were the independent risk factors affecting the prognosis of the patients with pancreatic head carcinoma(P<0.05). Conclusion　Survivin is highly expressed in the tissue of pancreatic head carcinoma, which is closely related to the occurrence, development and prognosis of pancreatic head carcinoma.]]> 2019/10/30 10:56:24 JIA Fu-xin, LIU Meng-meng, LIU Jiang-wei 98true ［Abstract］　Objective　To explore the efficiency and safety of minocycline and other combination drugs plus modified ephedra cimicifugae decoction on multidrug-resistant Acinetobacter baumannii(MDRAB) pneumonia. Methods　Thirty-four patients with pneumonia caused by multi-drug resistant acinetobacter baumannii were collected from the ICU and ICU observation rooms of the hospital during January 2013 and March 2018 and were divided into the treatment group and the control group, with 17 cases in each group. Secretions of the lower respiratory tract were collected from the thirty-four patients and MDRAB were isolated from the secretions. In the control group, minocycline(0.2 g/d, first dose doubled) combined with cefoperazone-sulbactam large dose(12 g/d), or combined with piperacillin-tazobactam(11.25 g/d) was given to the patients. The treatment group was treated with the same treatment as the control group plus Traditional Chinese Medicine(TCM) modified ephedra cimicifugae decoction. Both groups were treated for 14 days as a course of treatment. After one course of treatment, the clinical total effective rate, bacterial clearance rate and the improvement of serum inflammatory markers were observed and compared between the two groups. Results　The therapeutic effect and the bacterial clearance rate of the treatment group were significantly higher than those of the control group(P<0.05). There were significant difference in serum inflammatory markers before and after treatment in the treatment group(P<0.05). Conclusion　Minocycline(0.2 g/d, first dose doubled) in combination with large dose of cefoperazone-sulbactam(12 g/d), or combined with piperacillin-tazobactam(11.25 g/d) in association with TCM ephedra cimicifugae decoction can significantly reduce the levels of serum inflammatory cytokines, improve the clinical effect of MDRAB pneumonia and eliminate the bacteria of Acinetobacter baumannii, and is worthy of clinical application.]]> 2019/10/30 10:56:24 XU Cheng-yong, XIE Wei-xin, WU Chun-hong, et al. 97true ［Abstract］　Objective　To explore the possible causes and mechanism of proton pump inhibitors(PPIs) in treatment of gout attack in patients with stable gout and upper gastrointestinal hemorrhage. Methods　Twenty patients with stable gout and upper gastrointestinal hemorrhage were selected as the observation group and 20 patients with hyperuricemia and upper gastrointestinal hemorrhage who had no history of gout in the same period were selected as the control group. The urinary pH values, blood uric acid and electrolytes before and after PPIs treatment, and gout attacks after treatment were compared between the two groups. Results　During the treatment period, 20 patients in the observation group had acute episodes of gout. The time from using PPIs to clinical onset of acute gouty arthritis was 1 to 8 days, with an average of (3.95±1.79)days. The manifestations were fever, redness and swelling of joints, and pain and dysfunction of joints in some degree, but these symptoms did not appear in the control group. The blood uric acid levels were significantly increased after treatment compared with those before treatment in the two groups respectively(P<0.05). The levels of serum K⁺ and Ca²⁺ in the two groups were significantly lower after treatment than those before treatment(P<0.05). The levels of serum Cl^- in the two groups were significantly increased after treatment compared with those before treatment(P<0.05). There were no significant differences in the levels of Na⁺ in the two groups after treatment compared with those before treatment(P>0.05). Conclusion　PPIs may inhibit the renal tubular H⁺-K⁺-ATPase to some extent or impair the function of renal tubules, which may cause recurrence or aggravation of gout.]]> 2019/10/30 10:56:24 LI Yu-ling, NONG Bing, HUANG Zong-sheng, et al. 96true ［Abstract］　Objective　To investigate the effect of body temperature changes on hemostasis using tranexamic acid（TXA） during the perioperative period of total hip arthroplasty（THA）. Methods　Sixty patients receiving unilateral THA for the first time in our hospital from November 2016 to September 2018 were selected. The patients were divided into study group and control group according to the order of hospitalization, with 30 cases in each group. In the study group, the medical controllable thermal insulation blanket, the medical transfusion and infusion warmer, and the adjustment of the operating room ambient temperature were used to keep the patients′ body temperature above 36 ℃. However, the control group did not take any special temperature protection measures. The blood loss during the operation and the postoperative blood transfusion rate were compared between the two groups. Results　The intraoperative blood loss, amount of postoperative drainage and occult blood loss in the study group were significantly less than those in the control group(P<0.05). The hemoglobin levels of the study group were significantly higher than those of the control group immediately after the operation and 3 days after the operation. The postoperative prothrombin time in the study group was significantly shorter than that in the control group(P<0.05). The postoperative blood transfusion rate of the study group was significantly lower than that of the control group(P<0.05). Conclusion　Maintaining the normal intraoperative body temperature of THA patients can promote the hemostatic effect of TXA, reduce intraoperative and postoperative blood loss and recessive blood loss, and reduce the rate of blood transfusion.]]> 2019/10/30 10:56:24 HUANG Yu, LUO Yi, HUANG Xiao, et al. 95true ［Abstract］　Objective　To explore the clinical effect of pituitrin combined with misoprostol on laparoscopic myomectomy. Methods　Eighty patients with intramural hysteromyoma were collected from June 2016 to December 2017 and were randomly divided into the observation group(n=40) and the control group(n=40). In the observation group, misoprostol was placed in the anus 30 min before operation, and pituitrin was injected intraoperatively. In the control group, oxytocin was injected intraoperatively. The operative time, intraoperative blood loss, intraoperative heart rate, blood pressure, and postoperative hemoglobin, anal exhaust time, recovery time of body temperature, hospitalization time and adverse reactions were compared between the two groups. Results　The operation time in the observation group was significantly shorter than that in the control group(P<0.05). The intraoperative blood loss of the observation group was significantly less than that of the control group(P<0.05). There were no significant differences in the heart rate, systolic pressure and diastolic pressure between the two groups（P>0.05）. The level of hemoglobin in the observation group was significantly higher than that of the control group（P<0.05）. There were no significant differences in the postoperative anus exhaust time, recovery time of body temperature and hospitalization time between the two groups（P>0.05）. No adverse reactions occurred in the two groups after operation. Conclusion　Pituitrin in combination with misoprostol can significantly shorten the operative time and reduce the intraoperative blood loss during laparoscopic myomectomy, which is worthy of clinical application.]]> 2019/10/30 10:56:24 PENG Hua, HE li, OU Wen-mei, et al. 94true ［Abstract］　Objective　To observe the efficacy of oxaliplatin and fluorouracil combined with epirubicin in treatment of primary liver cancer complicated with HIV/AIDS via transcatheter arterial chemoembolization(TACE). Methods　One hundred patients with primary liver cancer complicated with HIV/AIDS treated in our hospital from June 2013 to December 2018 were divided into observation group and control group by random number table method, with 50 cases in each group. The observation group was treated with oxaliplatin and fluorouracil combined with epirubicin via TACE. The control group was treated with oxaliplatin and fluorouracil through TACE. The clinical efficacy and adverse reactions were compared between the two groups. Results　The clinical efficacy of the observation group was better than that of the control group(P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups(P>0.05). Conclusion　Oxaliplatin and fluorouracil combined with epirubicin is effective and safe in treatment of primary liver cancer complicated with HIV/AIDS via TACE. The combination therapy is worthy of clinical application.]]> 2019/10/30 10:56:24 LAN Zhi, LIANG Mao-yu, MO Wei-jie 93true ［Abstract］　Objective　To explore the correlation between serum concentrations of granulocyte-macrophage colony stimulating factor(GM-CSF) and interleukin-18(IL-18) in acute kidney injury(AKI)and their predictive roles in AKI. Methods　Thirty specific pathogen free(SPF) Wistar rats were selected and divided into sham operation group(SHAM group, n=5) and AKI model group(AKI group, n=25) by random number table method. The varied levels of serum creatinine(SCr), IL-18 and GM-CSF were detected in the two groups at the time points of 0 h, 24 h, 48 h, 72 h and 96 h, and their correlation was analyzed. Results　After modeling, renal tubulointerstitial cell necrosis and inflammatory cell infiltration occurred in the AKI group. The scores of renal tubular injury in the AKI group at the time points of 0 h, 24 h, 48 h, 72 h and 96 h were increased at first and then decreased. The scores of renal tubular injury at the time point of 48 h were the highest. The scores of renal tubular injury in the AKI group from 24 h to 96 h time points were higher than those in the SHAM group, and the differences were statistically significant(P<0.05). The levels of SCr in the AKI group showed a trend of continuous increase over time, while the levels of IL-18 and GM-CSF increased at first and then decreased. The levels of SCr, IL-18 and GM-CSF in the SHAM group did not change significantly at different time points. The levels of SCr in the AKI group were significantly higher than those in the SHAM group 48, 72 and 96 hours after modeling(P<0.05). The levels of IL-18 and GM-CSF in the AKI group were significantly higher than those in the SHAM group 48 and 72 hours after modeling(P<0.05). The results of Pearson correlation analysis showed that the level of serum IL-18 was positively correlated with SCr and GM-CSF in the AKI group(P<0.05). Conclusion　In the rat AKI model, serum GM-CSF is positively correlated with IL-18, and its peak value appears earlier than that of SCr, which can be used as a marker to predict the occurrence of AKI.]]> 2020/9/30 16:48:19 LIN Xin, LIU Juan, DONG Rong, et al. 92true ［Abstract］　Objective　To compare the application effects of endoscopic nasobiliary drainage(ENBD) and endoscopic biliary stenting(EBS) on patients with hilar cholangiocarcinoma before operation or before palliative treatment. Methods　One hundred and fifty-six patients with hilar cholangiocarcinoma who underwent temporary endoscopic biliary drainage at the Endoscopy Center of the First Affiliated Hospital of Henan University of Science and Technology from May 2011 to May 2019 were selected, and they were divided into ENBD group(112 cases) and EBS group(44 cases) according to different treatment methods. The levels of liver function indexes were compared between the two groups before and after treatment, as well as the incidence rates of postoperative drainage tube displacement, catheter blockage, cholangitis, cholecystitis, liver abscess, catheter-induced duodenal ulcer and complications of pancreatitis after endoscopic retrograde cholangiopancreatography(ERCP). Results　There were no statistically significant differences in the levels of alkaline phosphatase(ALP), total bilirubin(TB), alanine aminotransferase(ALT) and gamma glutamyl transferase(GGT) between the two groups before operation(P>0. 05). The levels of ALP, TB, ALT and GGT in the two groups were all decreased after operation, and the differences were statistically significant compared with those in the same group before operation(P<0.05), but the differences between the two groups were not statistically significant(P>0.05). The incidence rates of drainage tube displacement and cholecystitis in the ENBD group were lower than those in the EBS group, and the differences were statistically significant(P<0.05). However, there were no statistically significant differences in the incidence rates of drainage tube obstruction, cholangitis, liver abscess, catheter-induced duodenal ulcer and post-ERCP pancreatitis between the two groups(P>0.05). Conclusion　Both ENBD and EBS can significantly improve the patients′ liver function, but the incidence of postoperative complications of ENBD is lower. ENBD can be used as the first choice of temporary endoscopic biliary drainage for patients with hilar cholangiocarcinoma before operation or before palliative treatment.]]> 2020/9/30 16:48:19 JIANG Zhen, ZHANG Ying-jian, LIU Hai-chao 91true ［Abstract］　Objective　To analyze the effect of transumbilical single-port laparoscopic surgery on children with complicated appendicitis. Methods　Eighty-six children with complicated appendicitis admitted to our hospital from July 2017 to October 2019 were selected and divided into laparotomy group and transumbilical single-port laparoscopic group by random number table method, with 43 cases in each group. The laparotomy group was treated with conventional laparotomy, and the transumbilical single-port laparoscopic group received transumbilical single-port laparoscopic surgery. The treatment effect was compared between the children in the two groups. Results　The operation time, surgical incision length, postoperative anal exhaust time, time for first ambulation, drainage time, and hospitalization time in the transumbilical single-port laparoscopic group were significantly shorter than those in the laparotomy group(P<0.05), and the intraoperative blood loss in the transumbilical single-port laparoscopic group was significantly less than that in the laparotomy group(P<0.05). The postoperative Visual Analogue Scale(VAS) pain scores, utilization rate of analgesics, and the incidence of complications in the transumbilical single-port laparoscopic group were significantly lower than those in the laparotomy group(P<0.05). Compared with those before surgery, the levels of white blood cells(WBC) and C-reactive protein(CRP) in the two groups were significantly decreased three days after surgery(P<0.05). However, there were no significant differences between the two groups in the levels of WBC and CRP before surgery and three days after surgery(P>0.05). Conclusion　Transumbilical single-port laparoscopic surgery has a good clinical effect on treatment of complicated appendicitis in children. It can accurately locate the necrotic appendixes, effectively reduce the postoperative pain, and makes the patients has less postoperative complications and rapid postoperative recovery.]]> 2020/9/30 16:48:19 WAN Cheng-liang, HAI Bo, BAI Qiang, et al. 90true ［Abstract］　Objective　To investigate the intervention effect of potassium sodium hydrogen citrate on the development of clinically insignificant residual fragments(CIRF) after percutaneous nephrolithotomy. Methods　Sixty upper urinary calculus patients diagnosed with CIRF after percutaneous nephrolithotomy were included in this study and were randomly divided into intervention group(n=31 cases) and control group(n=29 cases). Percutaneous nephrolithotomy was performed in both groups. After the operation, the patients in the intervention group were given potassium sodium hydrogen citrate granules 10 g per day for 3 months, while the patients in the control group were not given any drugs. The incidence of clinical symptoms and the disappearance or growth of stones were compared between the two groups. Results　At twelve months after the operation, the incidence of residual stone increase in volume was 12.90% in the intervention group and 62.07% in the control group, and the incidence of residual stone disappearance was 41.94% and 17.24% respectively, and the incidence of residual stone decreased or unchanged was 45.16% and 20.69% respectively, with significant differences between the two groups(P<0.05). The incidence of residual stone related renal colic was 6.45% in the intervention group and 31.03% in the control group, with a significant difference between the two groups(P<0.05). The incidence of gross hematuria was 25.81% in the intervention group and 51.72% in the control group, with a significant difference between the two groups(P<0.05). Conclusion　Potassium sodium hydrogen citrate can significantly interfere with the development of stones in patients with CIRF after percutaneous nephrolithotomy, inhibit the growth of CIRF, promote the discharge of CIRF, and reduce the renal colic caused by CIRF.]]> 2020/9/30 16:48:19 WEI Xiu-wang, WANG Wei, CAI Bin, et al. 89true ［Abstract］　Objective　To analyze the serum ferritin(SF) and liver iron concentration(LIC) in splenectomised and non-splenectomised patients with hemoglobin H Constant Spring(HbH CS) disease, and to explore their correlation. Methods　Sixty-four patients with HbH CS disease treated in our hospital from July 2014 to June 2015 were selected and divided into the splenectomy group(n=34) and the non-splenectomy group(n=30) according to whether to receive splenectomy or not. The levels of hemoglobin(Hb), nucleated red blood cells(NRBC), reticulocytes(Ret), white blood cells(WBC) and SF, and hepatic magnetic resonance imaging(MRI) T2^* were compared between the two groups. The indicators correlated with lnSF(natural logarithm of SF) and hepatic MRI T2^* were screened. Results　The levels of Hb, WBC and NRBC in the splenectomy group were significantly higher than those in the non-splenectomy group(P<0.05). The values of lnSF and hepatic MRI T2^* in the splenectomy group were significantly higher than those in the non-splenectomy group(P<0.05), reflecting that the level of SF in the splenectomy group was higher than that in the non-splenectomy group, while the level of LIC in the splenectomy group was lower than that in the non-splenectomy group. In the splenectomy group, hepatic MRI T2^* was negatively correlated with age and lnSF(P<0.05); lnSF was negatively correlated with Hb(P<0.05), and positively correlated with age, NRBC, WBC, and blood transfusion levels(P<0.05). In the non-splenectomy group, hepatic MRI T2^* was negatively correlated with lnSF(P<0.05), and lnSF was positively correlated with age(P<0.05). Conclusion　 Hepatic MRI T2^* can reflect iron load in the liver. Inconsistency between SF level and LIC exists in the patients with HbH CS disease after splenectomy. Evaluation of iron load in the patients with thalassemia requires a comprehensive consideration of whether the patients have received splenectomy and the iron load in the viscera.]]> 2020/9/30 16:48:19 NIE Wei-ye, YIN Xiao-lin,YANG Jie, et al. 88true ［Abstract］　Objective　To explore the application value of transrectal real-time tissue elastography(TRTE) in evaluation of the change of tissue hardness and the degree of bladder outlet obstruction in benign prostatic hyperplasia(BPH), and to analyze the correlation between the indexes of prostate tissue hardness and the degree of bladder outlet obstruction. Methods　A total of 111 patients visiting the Department of Andrology, the Fifth Affiliated Hospital of Guangxi Medical University and undergoing TRTE examination from June 2018 to February 2020 were selected as the study subjects, including 81 patients with BPH(BPH group) and 30 patients without BPH(non-BPH group). The patients′ prostate elasticity images were obtained by TRTE, and the elasticity scores, strain of the external glands, strain of the internal glands and strain ratio(SR) were compared between the two groups. The correlation between AG value and the strain of the external glands, strain of the internal glands, SR and elasticity scores was analyzed by Spearman rank correlation analysis in the BPH group. Results　The strain of the external glands, SR and elasticity scores in the BPH group were significantly higher than those in the non-BPH group(P<0.05). In the BPH group, the strain of the internal glands ［0.13(0.10, 0.17)］ was smaller than that of the external glands ［0.32(0.24, 0.42)］, and the difference was statistically significant(Z=-7.569, P=0.000). The results of Spearman rank correlation analysis showed that the AG value was negatively correlated with the strain of the internal glands(P<0.05), and positively correlated with the SR and elasticity scores(P<0.05) in the BPH group. Conclusion　TRTE can be used to evaluate the elastic texture of prostate tissues. The occurrence of BPH increases the hardness of prostate tissues. The strain of the internal glands, SR and elasticity score are correlated with the degree of bladder outlet obstruction.]]> 2020/9/30 16:48:19 ZHENG Wen, PANG Guang-lian, SHEN Chun-mei, et al. 87true ［Abstract］　Objective　To analyze the changes in quality of life before and after anterior cervical discectomy and fusion(ACDF) in patients with multi-segment cervical spondylosis and the influencing factors of health improvement. Methods　One hundred and forty-three patients with multi-segment cervical spondylosis who underwent ACDF in our hospital from February 2010 to February 2017 were selected for evaluating the quality of life before and after operation using the Short-Form-36 Health Survey(SF-36) Scale. The influencing factors of postoperative health improvement of the patients were analyzed. Results　The quality of life of the patients with multi-segment cervical spondylosis before ACDF was generally limited, and ACDF could significantly improve the quality of life for the patients. Japanese Orthopedics Association(JOA) scores <9 points(OR=2.357), education level of junior high school and below(OR=1.645), course of the disease >6 years(OR=3.122), age >60 years(OR=5.049), and insecure job(OR=2.616) were the risk factors affecting health improvement of the patients with multi-segment cervical spondylosis after ACDF. Conclusion　ACDF can significantly improve the quality of life of the patients with multi-segment cervical spondylosis, and more nursing care should be given to the patients with the related risk factors affecting their postoperative improvement to effectively improve the quality of life after the operation.]]> 2020/9/30 16:48:19 HE Ji-chen, LI Wen-hao, TANG Si-gui, et al. 86true ［Abstract］　Objective　To explore the application effect of self-developed umbrella-type gasless device on laparoscopic cholecystectomy with single incision. Methods　Thirty-nine experimental pigs were randomly divided into the experimental group(n=20, laparoscopic cholecystectomy using umbrella-type gasless device with single incision) and the control group(n=19, laparoscopic cholecystectomy using conventional pneumoperitoneum). The operation time, intraoperative blood loss, postoperative hypersensitive C-reactive protein(hs-CRP), length of surgical incision and intraoperative complications were compared between the two groups. Results　The operations in both groups were completed successfully, and there were no conversion to open surgery, and no serious complications such as major bleeding and organ damage during the operations. The operation time of the experimental group was longer than that of the control group, and the difference was statistically significant(P<0.05). There were no significant differences between the two groups in the intraoperative blood loss, the levels of hs-CRP before and after operation, and the length of surgical incision(P>0.05). Conclusion　Compared with the conventional pneumoperitoneum surgery, the self-developed umbrella-type gasless device is safe and feasible for laparoscopic cholecystectomy with single incision, and the surgical wound is located in the umbilicus when the self-developed umbrella-type gasless device with single incision is used, and its cosmetic effect is better.]]> 2020/9/2 15:08:58 WU Dong-bo, LIN Hui-ling, YE Mao-ying, et al. 85true ［Abstract］　Objective　To compare the differences between the effects of ropivacaine and lidocaine on the degrees of intraoperative bleeding and postoperative swelling in double-eyelid plasty. Methods　Thirty-seven cases of beauty seekers who underwent bilateral blepharoplasty under local anesthesia in our hospital from January 2019 to March 2019 were selected. The patients′ left eyes were given lidocaine for local anesthesia and their right eyes were given ropivacaine for local anesthesia. The anesthetic dosage during operation, the bleeding score and the degree of swelling after operation were compared between the two groups. Results　There was no significantly difference in the anesthetic dosage between the left and the right eyes(P>0.05). The bleeding scores of the left eyes were significantly higher than those of the right eyes ［(3.24±0.64)points vs (2.78±0.53)points, P<0.05)］. The postoperative swelling scores of the left and the right eyes showed a downward trend, but the postoperative swelling scores of the right eyes at each time point were significantly lower than those of the left eyes(P<0.05). The swelling of all beauty seekers subsided naturally within 1 month. Conclusion　Ropivacaine can effectively reduce intraoperative bleeding and postoperative swelling in double-eyelid plasty.]]> 2020/9/2 15:08:58 QIANG Shuai, ZHOU Yu, CAO Yu-jiao, et al. 84true ［Abstract］　Objective　To study the association between NEDD4 gene polymorphism and scar pregnancy. Methods　Forty pregnant women who visited our hospital from January 2018 to September 2019 were selected as the research subjects. According to their ultrasound results, the patients were divided into the scar pregnancy group and the non-scar pregnancy group, with 20 cases in each group. Blood samples were drawn from cubital veins for extraction of DNA samples. The genetic loci of rs2271289, rs2303579 and rs10518830 of the NEDD4 gene were sequenced. The genotype and allele frequencies were compared between the two groups. Results　The genotype and the allele frequency of rs2271289 locus were significantly different between the two groups(P<0.05). Compared with the TT genotype at rs2271289 locus, CC genotype was a risk factor for promoting scar pregnancy(OR=24.00, 95%CI: 1.74～330.80); compared with the T allele at rs2271289 locus, the C allele was a risk factor for promoting scar pregnancy(OR=3.12, 95%CI: 1.25～7.78). The genotypes and allele frequencies of rs2303579 locus and rs10518830 locus were not significantly different between the two groups(P>0.05). Conclusion　The CC genotype and the C allele at rs2271289 locus of NEDD4 gene may be the risk factors for promoting scar pregnancy.]]> 2020/9/2 15:08:58 SHE Qin, PENG Cui, HE Qian-ying, et al. 83true ［Abstract］　Objective　To investigate the effect of anterolateral thigh flap anatomical repair on speech function recovery after reconstruction of tongue carcinoma defects. Methods　Fifty-eight patients with tongue cancer admitted to our hospital from January 2018 to January 2020 were selected, and were randomly divided into the anatomical repair group and the non-anatomical repair group by random number table method, with 29 cases in each group. The anterolateral thigh flap anatomical repair and non-anatomical repair were used to repair the postoperative defects of tongue cancer for the two groups respectively. The tongue muscle motor score, the phonetic intelligibility(PI) of apical sound, lateral sound and velar sound were compared between the two groups 1 month, 3 months and 6 months after operation. Results　The tongue muscle motor scores showed a downward trend in both groups after operation. The tongue muscle motor scores of the anatomical repair group were significantly higher than those of the non-anatomical repair group 1 month, 3 months and 6 months after operation, and the differences were statistically significant(P<0.05). The PI values of apical sound, lateral sound and velar sound showed a downward trend in both groups at different time points after operation. The PI values of apical sound and velar sound in the anatomical repair group were significantly higher than those in the non-anatomical repair group at different time points after operation(P<0.05). However, for the lateral sound, the PI value of the anatomical repair group was significantly higher than that of the the non-anatomical repair group only at the first month after operation(P<0.05). Conclusion　Compared with non-anatomical repair, anatomical repair used for repairing postoperative defects of tongue cancer is helpful to reconstruct the shape of the tongue, restore the movement of the tongue and improve the postoperative speech function of the patients with tongue cancer.]]> 2020/9/2 15:08:58 LI Shuai, WANG Xiao-meng, WEI Yi, et al. 82true ［Abstract］　Objective　To investigate the effects of Duhuo Jisheng decoction on the levels of interleukin-10(IL-10), interleukin-17(IL-17), interleukin-37(IL-37) and interferon gamma(IFN-γ) in the serum of collagen-induced arthritis(CIA) rats. Methods　Sixty male Wistar rats were selected, among which six rats were randomly selected as the normal group, and the other 54 rats were injected with complete Freund′s adjuvant(CFA) to construct a CIA model. Thirty CIA model rats were selected and randomly divided into positive control group, celecoxib group(intervened with celecoxib at 0.05 g/ml) and high-, medium-, and low-dose Duhuo Jisheng decoction groups(intervened with Duhuo Jisheng decoction at 4.40 g/ml, 2.20 g/ml and 1.10 g/ml respectively). The serum levels of IL-17, IFN-γ, IL-10 and IL-37 were compared among the groups after intervention. Results　Compared with those in the normal group, the levels of IL-17 and IFN-γ in the positive control group were significantly increased(P<0.05). Compared with those in the positive control group, the levels of IL-10 and IL-37 increased, and the levels of IL-17 and IFN-γ decreased in the celecoxib group and the medium-dose Duhuo Jisheng decoction group, and the differences were statistically significant(P<0.05); However, there were no significant differences between the positive control group and the high- and low- dose Duhuo Jisheng decoction groups(P>0.05). Conclusion　Duhuo Jisheng decoction has significant improvement on arthritis in CIA rats, and its anti-inflammatory mechanism may be achieved by reducing the levels of pro-inflammatory factors of IL-17 and IFN-γ, and increasing the levels of anti-inflammatory factors of IL-10 and IL-37.]]> 2020/9/2 15:08:58 YUAN Yu-ying, LI Hao, ZHANG Zheng-wu, et al. 81true ［Abstract］　Objective　To compare the effects of different sutures and suture methods on laparoscopic myomectomy(LM) during and after operation and pregnancy outcomes. Methods　One hundred and seventy-four patients with uterine myometrial fibroids receiving LM treatment in our hospital from January 2013 to March 2018 were selected. According to different sutures and suture methods, the patients were divided into the common absorbable pad suture group(n=34), the common absorbable baseball suture group(n=34), the V-Loc pad suture group(n=55) and the V-Loc baseball suture group(n=51). The intraoperative blood loss, the operation time, the degree of hemoglobin(Hb) decrease after operation, the rate of residual myoma and pregnancy were compared among the four groups. Results　There were statistically significant differences in the operation time, the intraoperative blood loss and the degree of Hb decrease among the four groups(P<0.05). During the follow-up period, 52 cases were pregnant successfully, with a total of 59 times of pregnancy, and the pregnancy rate was 29.89%(52/174). Among them, 5 cases had a full-term pregnancy once again after the first pregnancy abortion; 1 case had a full-term pregnancy after 2 times of miscarriage. There were no significant differences in the pregnancy rate and pregnancy interval among the four groups(P>0.05). There were no complications during and after operation in the four groups. No residual fibroids were found in B-ultrasound examination 3 months after operation. Conclusion　Using V-Loc baseball suture can shorten the operation time and reduce the amount of intraoperative and postoperative bleeding. Different sutures and suture methods have certain impacts on the patients′ pregnancy outcomes after LM.]]> 2020/9/2 15:08:58 LIU Ni-ping, ZHAO Ren-feng, WEI Hai-tang, et al. 80true ［Abstract］　Objective　To explore the application value of bedside Gugging swallowing screen(GUSS) in the quality management of single disease of acute cerebral infarction. Methods　Four hundred patients with acute cerebral infarction who were hospitalized in our hospital from January 2015 to December 2015 were selected. All the patients completed GUSS scores within 48 hours of admission. According to the patients′ GUSS scores, dietary recommendations and swallowing rehabilitation treatment were performed. The recovery of swallowing function, the length of hospitalization, the incidence of pneumonia, the utilization rate of antibiotics, and the outcome at discharge were compared among the groups with different degrees of dysphagia. Results　All the 400 patients with acute cerebral infarction completed the first bedside GUSS within 48 hours of admission, with an implementation rate of 100%. The bedside GUSS results showed that the incidence of dysphagia in the patients with acute cerebral infarction was 47.50%(190/400), including 88 cases(46.32%) of mild dysphagia, 60 cases(31.58%) of moderate dysphagia, and 42 cases(22.10%) of severe dysphagia. The TOAST classification of severe dysphagia was mainly cardiogenic cerebral embolism; the TOAST classification of moderate dysphagia was mainly large arteries atherosclerotic thrombosis, and the TOAST classification of mild dysphagia was mainly large arteries atherosclerotic thrombosis and lacunar cerebral infarction. Compared with those before the intervention treatment, the GUSS scores of the patients with mild, moderate and severe dysphagia were higher one week after the intervention treatment, and the differences were statistically significant(P<0.05). The rate of pneumonia during hospitalization, the length of hospital stay, the utilization rate of antibiotics and the adverse outcome rate at discharge in the dysphagia group were greater than those in the non-dysphagia group, and the differences were statistically significant(P<0.05). Conclusion　Bedside GUSS is easy to operate and can objectively evaluate the risk of aspiration and the severity of dysphagia in patients with acute cerebral infarction, reduce the incidence of post-stroke pneumonia, and promote the patients′ recovery.]]> 2020/9/2 15:08:59 JIANG Liu-jie, JIANG Hong-bo, PENG Jun, et al. 79true ［Abstract］　Objective　To analyze the characteristics and related factors of medical coping modes in alcohol dependent patients. Methods　Eighty-nine alcohol dependent patients admitted to our hospital were selected from December 2018 to June 2019. The self-made general information questionnaire and the Medical Coping Modes Questionnaire(MCMQ) were used as the research tools to evaluate the patients′ clinical characteristics and medical coping modes, and the evaluated results were compared with those of the national norm of China. The correlation between the patients′ clinical characteristics and medical coping modes was analyzed. Results　The scores of “confronce” item in the 89 patients with alcohol dependence were lower than those in the national norm, while the scores of “avoidance” and “resignation” items in the 89 patients with alcohol dependence were higher than those in the national norm, and the differences were statistically significant(P<0.05). The years of drinking alcohol of the alcohol dependent patients was positively correlated with the scores of “resignation” item(P<0.05). Conclusion　Alcohol dependent patients are less likely to adopt the medical coping mode of “confronce” in the face of illnesses and are more likely to adopt “avoidance” and “resignation” coping modes. With the extension of drinking years, the patients tend to adopt “resignation” coping mode. Clinicians should encourage the patients to adopt positive medical coping modes to improve the treatment effect and the mental health of the alcohol dependent patients.]]> 2020/9/2 15:08:59 TAO Guang-yuan, ZHANG Ling, CAO Yong-kang, et al. 78true ［Abstract］　Objective　To investigate the correlation between the expression level of tumor necrosis factor-α(TNF-α) in middle ear cholesteatoma epithelium and the hearing loss of patients. Methods　Eighty-nine patients with chronic otitis media admitted to the People′s Hospital of Xinjiang Uygar Autonomous Region from January 2017 to January 2020 were selected, including 68 patients with middle ear cholesteatoma(observation group) and 21 patients with active chronic suppurative otitis media(control group). The preoperative air conduction threshold, bone conduction threshold and bone air conduction difference were compared between the two groups. At the entrance of the middle ear tympanic sinus, the cholesteatoma epitheliums were taken from the observation group and the mucosal tissues of middle ear lesions were taken from the control group. The expression levels of TNF-α in tissue samples from the two groups were compared. The correlation between the expression level of TNF-α in the cholesteatoma epithelium of the middle ear and the hearing loss of the patients was analyzed. Results　The levels of air conduction threshold and bone air conduction difference in the observation group were significantly higher than those in the control group(P<0.05); but there was no statistically significant difference in the level of bone conduction threshold between the two groups(P>0.05). The expression level of TNF-α in the cholesteatoma epitheliums in the observation group was (18.64±8.55)ng/L and that in the mucosal tissues of middle ear lesions at the entrance of the middle ear tympanic sinus in the control group was (28.29±5.64)ng/L, with a significant difference between the two groups(P<0.05). The level of TNF-α in cholesteatoma epithelial tissues was positively correlated with air conduction threshold, bone conduction threshold and bone air conduction difference(P<0.05). Conclusion　The expression level of TNF-α in the cholesteatoma epithelium of the middle ear is positively correlated with the patients′ hearing loss.]]> 2020/8/3 9:08:34 HUANG Xiao-bang, TONG Fu-lin, CHENG Xiu-qin, et al. 77true ［Abstract］　Objective　To investigate the effect of dexmedetomidine injection on postoperative delirium in patients with Stanford type A aortic dissection undergoing total arch replacement surgery. Methods　A total of 117 patients with Stanford type A aortic dissection undergoing total arch replacement surgery from June 2015 to June 2017 in our hospital were selected. The patients were randomly divided into the dexmedetomidine group(n=59) and the propofol group(n=58) by random number table method. Both groups underwent the same anesthesia and surgery. After admission to the Intensive Care Unit(ICU), the dexmedetomidine group was intravenously pumped with dexmedetomidine 0.40～0.80 μg/(kg·h) until 30 minutes before extubation, while the propofol group was intravenously pumped with propofol 25～40 μg/(kg·min) until 2 hours before extubation. The intraoperative and postoperative indexes, the incidence of postoperative adverse reactions and the incidence of postoperative delirium were compared between the two groups. Results　The patients in the dexmedetomidine group had shorter mechanical ventilation time, wake-up time after drug withdrawal, ICU stay time and total hospital stay time than those in the propofol group, and the difference was statistically significant(P<0.05). There were no significant differences in the incidence rates of hypotension, bradycardia, and nausea/vomiting between the two groups(P>0.05). Compared with the propofol group, the dexmedetomidine group had lower incidence of delirium, later onset of delirium and shorter duration of delirium, with statistically significant differences between the two groups(P<0.05). Conclusion　Compared with propofol, dexmedetomidine can reduce mechanical ventilation time and ICU stay time in the patients with Stanford type A aortic dissection after total arch replacement surgery, reduce the incidence of delirium and shorten the total hospital stay.]]> 2020/8/3 9:08:34 CAO Bao-jiang, YE Fa-min, ZHANG Jing-jing, et al. 76true ［Abstract］　Objective　To investigate the nutritional status of serum vitamin A(VA) and vitamin D(VD) in infants and young children aged 0～3 years in Chaozhou area, and to analyze the correlation between the nutritional status of VA and VD and respiratory tract infection and some blood routine parameters. Methods　Six hundred and eighty-three children who had undergone health care and physical examination in a hospital in Chaozhou City, Guangdong Province from January 2016 to December 2017 were selected, and their general clinical data, serum VA, serum VD and routine blood test results were collected. The effects of different ages, body mass index Z score(BMI-Z) and duration of breastfeeding on serum VA and serum VD, as well as the correlation between nutritional status of VA and VD and respiratory tract infection and some blood routine parameters were analyzed. Results　The levels of VA and VD in the young children group(1～3 years) were higher than those in the infant group(<1 year), and the deficiency rate and the insufficience rate were also lower, and the differences were statistically significant(P<0.05). The levels of VA and VD in the BMI-Z<1 group were lower than those in the BMI-Z≥1 group, and the deficiency rate and the insufficience rate of VA and VD were higher, and the differences were statistically significant(P<0.05). There were no statistically significant differences in the levels of VA and VD and VA status between the breastfeeding duration ≥6 months group and the breastfeeding duration <6 months group(P>0.05). The deficiency rate and the insufficience rate of VD in the breastfeeding duration ≥6 months group were significantly lower than those in the breastfeeding duration <6 months group(P<0.05). There was no significant difference in the number of respiratory tract infection between the normal VA group and the VA deficiency/insufficience group(P>0.05); the number of respiratory tract infection in the VD deficiency/insufficience group was significantly higher than that in the normal VD group(P<0.05). The level of platelet in the normal VA group was significantly higher than that in the VA deficiency/insufficience group(P<0.05). The level of mean platelet volume in the normal VD group was significantly lower than that in the VD deficiency / insufficience group(P<0.05). Conclusion　Infants and young children aged 0～3 years in Chaozhou area have higher VA and VD deficiency and insufficience rates. Poor nutritional status and shorter breastfeeding time may cause VA and VD deficiency or insufficience in the infants and young children. The deficiency and insufficience of VA and VD may lead to the decrease of immune function by affecting platelet function, and the deficiency and insufficience of VD may increase the risk of respiratory tract infection in the infants and young children.]]> 2020/8/3 9:08:34 XIE Wei-bo, XIE Jun-bo, YIN Xiao-feng, et al. 75true ［Abstract］　Objective　To analyze the expressions of versican(VCAN) and serum cancer antigen 125(CA125) in patients with endometrioid adenocarcinoma(EA) and their correlations with clinicopathological characteristics. Methods　One hundred and twenty outpatients receiving endometrial tissue examination at our hospital from January 2016 to January 2019 were selected, including 60 patients with EA(EA group), 40 patients with atypical endometrial hyperplasia(atypical endometrial hyperplasia group), and 20 patients with normal endometrium(normal endometrial group). The levels of the patients′ VCAN and serum CA125 were compared among the three groups. The correlations between VCAN and serum CA125 expressions and clinicopathological characteristics were analyzed in the EA patients. Results　There were significant differences in the positive rates of VCAN and serum CA125 among the EA group, the atypical endometrial hyperplasia group and the normal endometrial group(P<0.05), and the positive rates of VCAN and serum CA125 of the EA group were the highest in the three group. The proportion of the VCAN positive expression EA patients with surgical grade Ⅲ/Ⅳ, infiltration depth >1/2 and lymph node metastasis was significantly higher than that of the VCAN negative expression EA patients(P<0.05). The proportion of the serum CA125 positive expression EA patients with surgical grade Ⅲ/Ⅳ and lymph node metastasis was significantly higher than that of the serum CA125 negative expression EA patients(P<0.05). There was significant difference between the expression of VCAN and the expression of serum CA125 in the EA patients(P=0.000). Conclusion　VCAN and serum CA125 play an important role in the occurrence and development of EA. They provide a reference for the early diagnosis and treatment options of EA.]]> 2020/8/3 9:08:34 LI Jing-ling, WEI Hai-ming, ZHAO Ren-feng, et al. 74true ［Abstract］　Objective　To analyze the clinical effect of deep scleral fixation with intraocular lens(IOL) under scleral flap. Methods　Sixty patients undergoing IOL fixation at our hospital from March 2017 to June 2019 were selected and were divided into the observation group(treated with deep scleral fixation with IOL under scleral flap) and the control group(receiving IOL with ciliary sulcus suture fixation) by random number table method, with 30 cases in each group. The visual acuity, intraocular pressure and corneal endothelial cell density were compared between the two groups before and after surgery, and the postoperative IOL position and complications were compared between the two groups. Results　There were no significant differences in preoperative visual acuity, intraocular pressure and corneal endothelial cell density between the two groups(P>0.05). The postoperative visual acuity recovery in the observation group was significantly better than that in the control group(P<0.05); however, there were no significant differences in intraocular pressure and corneal endothelial cell density between the two groups(P>0.05). In the observation group, there were 25 cases(83.33%) of orthotopic IOL and 5 cases(16.67%) of misaligned IOL after surgery. In the control group, there were 14 cases(46.67%) of orthotopic IOL and 16 cases(53.33%) of misaligned IOL after surgery. There were significant differences between the two groups in the rates of orthotopic IOL and misaligned IOL after surgery(P<0.05). No serious postoperative complications occurred in both groups. Conclusion　The IOL position after deep scleral IOL fixation under scleral flap is good, which is helpful for the recovery of visual acuity of the patients, and is worthy of clinical application.]]> 2020/8/3 9:08:34 WEI Le-qiang, FAN Jun, WU Dong-lian, et al. 73true ［Abstract］　Objective　To investigate the effect of recombinant human granulocyte colony-stimulating factor(rhG-CSF) on the expressions of inflammatory factors in young rats with acute lung injury. Methods　One hundred and twenty young Sprague-Dawley(SD) rats were selected and randomly divided into experimental group and control group, with 60 cases in each group.A model of acute lung injury was established by injection of refined lipopolysaccharide of escherichia coli endotoxin 5 mg/kg via tail vein. The experimental group was treated with subcutaneous injection of rhG-CSF 30 μg /(kg·d), and the control group was injected with the same volume of normal saline. The levels of transforming growth factor-β1(TGF-β1), tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) in lung tissues and bronchoalveolar lavage fluid were compared between the two groups on the 5th, 10th, and 14th days after the intervention. Results　On the 5th, 10th and 14th days after the experimental intervention, the levels of TGF-β1, TNF-α and IL-1β in bronchoalveolar lavage fluid and lung tissues in the experimental group were significantly lower than those in the control group(P<0.05). Conclusion　rhG-CSF can effectively reduce the body′s inflammatory response of young rats with acute lung injury.]]> 2020/8/3 9:08:34 MO Jin-li, HUANG Guo-ri, PAN Ge, et al. 72true ［Abstract］　Objective　To analyze the disease composition and changes of inpatients in the open ward of the psychology department of a tertiary psychiatric hospital in Guangxi from 2009 to 2018 for providing data support for the prevention, treatment and development planning of psychiatric diseases in the future. Methods　The data of medical records of 8 478 inpatients with mental disorders in the Department of Psychology, Nanning Fifth People′s Hospital from 2009 to 2018 were collected, and the change trend of different diseases was retrospectively analyzed. Results　From 2009 to 2018, the majority of the inpatients with mental disorders were 4～40 years old. The number of male patients with schizophrenia and obsessive-compulsive disorder was more than that of female patients, while the number of female patients with other anxiety disorders, depressive episodes, somatization disorders, bipolar disorder, non-organic sleep disorders, brain damage and functional disorders, and other mental disorders and severe stress response and adaptation disorders caused by physical diseases was more than that of male patients, and the differences were statistically significant(P<0.05). The growing number of the inpatients with schizophrenia had slowed down since 2014, and the number of the schizophrenia inpatients decreased significantly in 2018. The proportion of the patients with anxiety disorder, depressive episodes, bipolar disorder and non-organic sleep disorders had an overall upward trend. Conclusion　Minors and young and middle-aged people are the high-risk population in the prevention and treatment of psychological diseases. Anxiety disorder, depressive episodes and non-organic sleep disorders are the key diseases in the prevention and treatment of psychological diseases.]]> 2020/8/3 9:08:34 HUANG Shu-jin, ZHANG Ling, ZHU Xiao-xi, et al. 71true ［Abstract］　Objective　To explore the clinical and pathological features of primary amyloidosis of the respiratory system, and to provide evidence for clinical diagnosis and treatment. Methods　The clinical data of 9 patients with primary amyloidosis in our hospital from January 2014 to December 2019 were retrospectively analyzed. The clinical and pathological characteristics, as well as the correlation between these characteristics and symptoms and prognosis were summarized. Results　Of the 9 cases, 3 cases were male and 6 cases were female, with a median age of 65 years. Six cases of respiratory tract lesions were focal and 3 cases were diffusely distributed. The results of hematoxylin-eosin(HE) staining showed that the powder-like sediments were pink with amorphous structures and characteristic cracks. Congo red staining showed that the amyloid was brick red, and the characteristic “apple green” birefringence was displayed under a polarized light microscope. Immunohistochemical staining suggested that plasma cells around the sediment restrictedly expressed immunoglobulin light chains. Conclusion　The clinical manifestations and auxiliary examinations of primary amyloidosis of the respiratory system lack specificity, and the prognosis is related to the type of the lesions. Pathological biopsy is a reliable diagnostic method to confirm the disease.]]> 2020/7/3 15:01:52 HUANG Yong-ta, YE Qiu-rong, LIU Xia, et al. 70true ［Abstract］　Objective　To explore the clinical effect of cervical nerve root block by lateral approach under the guidance of ultrasound on treatment of cervical spondylotic radiculopathy. Methods　One hundred and eight patients with cervical spondylotic radiculopathy treated in our hospital from February 2016 to June 2019 were selected as the research subjects. They were divided into observation group(55 cases) and control group(53 cases) according to different treatment methods. The control group was treated with anterior cervical approach for cervical nerve root block according to the anatomical location of the body surface, and the observation group was treated with ultrasound-guided cervical nerve root block through the lateral approach. The puncture time and puncture success rate were compared between the two groups, as well as the Visual Analogue Scale(VAS) score, Cervical Dysfunction Index(NDI) score, health status score, effective rate and complication rate after treatment. Results　The puncture success rate in the observation group was higher than that in the control group, and the puncture time in the observation group was shorter than that in the control group, and the differences were statistically significant(P<0.05). Before treatment, there were no significant differences in the VAS scores, NDI scores, Physical Health(PH) scores and Mental Health(MH) scores between the two groups(P>0.05). Compared with those before treatment, the VAS scores were significantly lower in the two groups 1, 3 and 6 months after treatment(P<0.05), and the VAS scores of the observation group were significantly lower than those of the control group(P<0.05). Compared with those before treatment, the NDI scores were significantly lower, and the PH scores and MH scores were significantly higher in the two groups 1, 3, and 6 months after treatment(P<0.05). The NDI scores in the observation group were significantly lower than those in the control group, and the PH scores and MH scores in the observation group were significantly higher than those in the control group 3 and 6 months after treatment(P<0. 05). The effective rate was 94.55% in the observation group and 79.25% in the control group, and the difference was statistically significant between the two groups(P<0.05). Conclusion　Under the guidance of ultrasound, the lateral approach of cervical nerve root block is effective in treatment of cervical spondylosis radiculopathy, with a high success rate of puncture, and has a broader clinical application prospect.]]> 2020/7/3 15:01:52 LIU Wu, SUN Yong, ZHANG Wei, et al. 69true ［Abstract］　Objective　To explore the effect of spironolactone on treatment of primary aldosteronism(PA), and to analyze the factors affecting clinical remission. Methods　The clinical data of 95 PA patients in our hospital from January 2015 to December 2018 were retrospectively analyzed. All the patients were treated with spironolactone. The improvements of blood pressure and blood potassium, and the changes of general clinical indexes were analyzed. The PA patients were divided into remission group(39 cases) and non-remission group(56 cases) according to different therapeutic effects. The general data and the results of biochemical index examination were compared between the two groups before treatment, and the factors affecting the clinical remission of the patients receiving spironolactone were analyzed after treatment. Results　After treatment, the clinical remission rate was 41.05%(39/95); the control rate of blood pressure was 49.47%(47/95) and the control rate of blood potassium was 72.63%(69/95). Compared with those before treatment, the levels of systolic pressure, diastolic pressure, and aldosterone to renin ratio(ARR) were lower, while the levels of abdominal circumference, blood potassium, seated renin, and seated aldosterone were higher, and the differences were statistically significant(P<0.05). The proportion of males, the proportion of the patients with hypokalemia, the proportion of the patients with seated plasma aldosterone ≥20 ng/dL, and the seated aldosterone level in the non-remission group were significantly higher than those in the remission group(P<0.05). The results of Logistic regression analysis showed that the combination of hypokalemia and plasma aldosterone ≥20 ng/dL before treatment was the influencing factor of the poor effect of spironolactone on PA(P<0.05). Conclusion　Hypokalemia and plasma aldosterone level ≥20 ng/dL in PA patients before treatment are the influencing factors leading to the poor therapeutic effect of spironolactone on PA, and clinicians need to strengthen the follow-up and intervention of these patients.]]> 2020/7/3 15:01:52 WANG Xiao-tong, LUO Qin, LI Nan-fang 68true ［Abstract］　Objective　To observe the blood pressure fluctuation in metabolic syndrome(MS) patients with normal clinic blood pressure, and to analyze the influencing factors of MS and the effects of MS on cardiovascular and cerebrovascular events. Methods　One hundred and twenty-five patients with suspected coronary heart disease admitted to the Department of Cardiology, Laiyang Central Hospital of Yantai City from February 2015 to September 2017 were selected and were divided into non-MS group(n=60) and MS group(n=65) according to whether or not MS occurred. The differences of general clinical indicators and ambulatory blood pressure parameters were compared between the two groups, and the influencing factors of MS and the effects of MS on the occurrence of major adverse cardio-cerebral vascular events(MACCE) were analyzed. Results　The levels of waist circumference, body mass index(BMI), triglyceride(TG), fasting blood glucose and glycated hemoglobin(HbA1c) in the MS group were higher than those in the non-MS group. The proportion of the patients complicated with diabetes and masked hypertension(MH) in the MS group was higher than that in the non-MS group, but the level of HDL-C in the MS group was lower than that in the non-MS group(P<0.05). The levels of 24-hour mean systolic blood pressure(24hSBP), daytime average systolic blood pressure(dSBP), nighttime average systolic blood pressure(nSBP), daytime systolic blood pressure plateau, nighttime systolic blood pressure plateau and increasing rate of early morning SBP, 24-hour mean arterial blood pressure(24hMAP), diurnal mean arterial blood pressure(dMAP) and nocturnal mean arterial blood pressure plateau in the MS group were significantly higher than those in the non-MS group(P<0.05). The results of Logistic regression analysis showed that larger waist circumference, higher levels of BMI and increasing rate of early morning systolic blood pressure were the risk factors that promoted the occurrence of MS, while higher levels of HDL-C were the protective factors that inhibited the occurrence of MS(P<0.05). Survival analysis with MACCE as the outcome showed that the risk of MACCE in the MS group was significantly higher than that in the non-MS group(P<0.05). Conclusion　The occurrence of MS increases the levels of systolic blood pressure and aggravates blood pressure fluctuations in the morning, and may further increase the risk of MACCE.]]> 2020/7/3 15:01:52 LYU Guo-ling, LI Xia, LIU Ai-ling, et al. 67true ［Abstract］　Objective　To observe the clinical effects of microvascular decompression(MVD) on primary trigeminal neuralgia and the changes of anxiety and depression in the primary trigeminal neuralgia patients before and after treatment. Methods　Eighty patients with primary trigeminal neuralgia who underwent MVD in the Department of Neurosurgery, the Second Affiliated Hospital of Guangxi Medical University from September 2014 to December 2018 were selected. Zung Self-rating Depression Scale(SDS) and Zung Self-rating Anxiety Scale(SAS) were used to score and compare the depression and anxiety of the patients before and 3 months after treatment. The clinical effect of MVD on primary trigeminal neuralgia was analyzed. Results　Of the 80 cases, 63 cases were cured, 13 cases markedly effective, 2 cases effective, 2 cases ineffective, and no recurrence was found after MVD treatment. Before treatment, of the 80 cases, 24 cases were depressed(30.00%) and 20 cases were anxious(25.00%); after treatment, 8 cases were depressed(10.00%) and 5 cases were anxious(6.25%), and the differences were statistically significant(P<0.05). Compared with those before treatment, SAS and SDS scores were significantly reduced after treatment(P<0.05). Conclusion　MVD is effective in treatment of primary trigeminal neuralgia, and the patients′ anxiety and depression symptoms are significantly improved.]]> 2020/7/3 15:01:52 GUO Xian-fang, LIAO Sheng-chao, MO Kai, et al. 66true ［Abstract］　Objective　To investigate the evaluating effect of two-dimensional echocardiography combined with automatic functional imaging(AFI) technology on left ventricular systolic function in patients with atrial fibrillation. Methods　Forty-one patients with atrial fibrillation admitted to our hospital from January 2016 to December 2019 were selected and were divided into the normal left ventricular ejection fraction(LVEF) group(LVEF≥50%, 20 cases) and the LVEF-reduced group(LVEF<50%, 21 cases). Other twenty subjects undergoing routine physical examination were selected as the control group. Left ventricular end diastolic diameter(LVEDd), left ventricular end systolic diameter(LVESd), global longitudinal peak strain of apical long-axis(GLPS-LAX),global longitudinal peak strain of apical four-chamber(GLPS-A4C), global longitudinal peak strain of apical two-chamber(GLPS-A2C) and average global longitudinal peak strain(GLPS-AVG) were compared among the three groups. The correlation between LVEF and GLPS-LAX, GLPS-A4C, GLPS-A2C, GLPS-AVG was analyzed. Results　The age, heart rate, LVEDd and LVESd levels in the normal LVEF group and the LVEF-reduced group were significantly higher than those in the control group(P<0.05). The levels of LVEDd and LVESd in the LVEF-reduced group were significantly higher than those in the normal LVEF group(P<0.05). The level of LVEF in the normal LVEF group was lower than that in the control group, but the level of LVEF in the normal LVEF group was higher than that in the LVEF-reduced group, and the differences were statistically significant(P<0.05). The levels of GLPS-LAX, GLPS-A4C, GLPS-A2C and GLPS-AVG parameters(absolute values) in the normal LVEF group and the LVEF-reduced group were significantly lower than those in the control group(P<0.05). Compared with those in the normal LVEF group, the parameters(absolute values) in the LVEF-reduced group were significantly lower(P<0.05). The results of Pearson correlation analysis showed that LVEF was negatively correlated with GLPS-LAX, GLPS-A4C, GLPS-A2C, and GLPS-AVG in the control group, the normal LVEF group, and the LVEF-reduced group(P<0.05). Conclusion　LVEF in the patients with atrial fibrillation only becomes abnormal when the left ventricles enlarge significantly, and AFI technology can diagnose the abnormal left ventricular systolic function earlier, providing technical support for the early detection and treatment of atrial fibrillation.]]> 2020/7/3 15:01:52 XU Juan, CHEN Qiu-lan, LI Xiao-xuan, et al. 65true ［Abstract］　Objective　To analyze the consistency of the diagnostic results of transvaginal four-dimensional hysterosalpingo contrast sonography(4D-HyCoSy) and laparoscopy in diagnosis of tubal patency, and to investigate the application value of 4D-HyCoSy in the evaluation of tubal patency. Methods　Sixty infertile patients(120 fallopian tubes) treated in our hospital from August 2018 to November 2019 were selected as the research subjects. All the subjects underwent transvaginal 4D-HyCoSy and laparoscopy to diagnose tubal patency and the consistency of the diagnostic results of the two methods was analyzed. The correlation between the pelvic diffusion of contrast media and ovarian wrapping and the patency of fallopian tubes during 4D-HyCoSy examination was analyzed. Results　Laparoscopic examination showed that there were 55 unobstructed tubes, 37 partially obstructed tubes and 28 completely obstructed tubes. The results of 4D-HyCoSy examination showed that there were 58 unobstructed tubes, 35 partially obstructed tubes and 27 completely obstructed tubes. The results of the two methods were highly consistent(Kappa=0.908, P=0.000). For the patients with tubal patency, most of the pelvic contrast media were evenly diffused(82.76%), and the ring-shaped wrapped ovaries were the main parts(91.38%). For those with partially obstructed tubes, most of the pelvic contrast media were unevenly diffused(88.57%), and the semicircle-shaped wrapped ovaries were the main parts(88.57%). For those with completely obstructed tubes, the pelvic contrast media were not diffused(100.00%), and non-wrapped ovaries were the main parts(92.59%). There was a correlation between the pelvic diffusion of contrast media and the condition of ovarian wrapping and the patency of fallopian tubes during transvaginal 4D-HyCoSy examinations(P<0.05). Conclusion　The transvaginal 4D-HyCoSy examination and the laparoscopy fluid examination have a high consistency in the evaluation of the patency of fallopian tubes, and have a high clinical application value.]]> 2020/7/3 15:01:52 PEI Hua-jie, ZHENG Hong-yu, HE Yan, et al. 64true ［Abstract］　Objective　To summarize the treatment experience of stent implantation for Stanford type B aortic dissection under local anesthesia. Methods　The data of medical records of 32 cases of type B aortic dissection who received thoracic endovascular aortic repair(TEVAR) in our hospital From May 2017 to May 2019 were retrospectively analyzed to summarize the treatment experience. Results　The operation was successfully completed in all the 32 cases. During the operation, a small amount of type Ⅰ endoleak occurred in 1 case, but no deaths and no major cardiovascular and cerebrovascular accidents occurred. The patients were followed up for 6.4 to 10.5 months. One patient had distal stent bulging 6 months after the operation and was cured by reimplantation of one stent. Conclusion　After careful preparation, evaluation and measurement of aortic dissection, TEVAR under local anesthesia for Stanford type B aortic dissection can be performed, and the occurrence of intraoperative and postoperative complications can be significantly reduced through the improvement of transverse inguinal incision or percutaneous puncture instead of longitudinal incision, as well as careful perioperative nursing.]]> 2020/7/3 15:01:52 SUN Yu-gui, ZHANG Li-fei, XIAO Zhi-hong 63true ［Abstract］　Objective　To explore the optimal enzyme-linked immunosorbent assay(ELISA) experimental conditions for detection of serum anti-calmodulin Calponin 2(CNN2) antibody. Methods　BL21(DE3) pLysS-pColdⅢ-C genetically engineered bacteria were recombinantly expressed and CNN2 was isolated and purified, and coated onto a microtiter plate. Serum samples from the patients with hepatocellular carcinoma(HCC) and normal controls were collected to investigate the concentration of protein coating, the selection of serum diluents, the selection of sealing methods and conditions, and the optimal conditions of horseradish peroxidase(HRP)-labeled avidin dilution. Results　When the concentration of CNN2 protein was 10 μg/ml, the concentration of protein coating was the optimal concentration. Using 0.5% bovine serum albumin(BSA) was the best sealing method. Using PBST to dilute serum had low costs and good results. The optimal dilution ratio of avidin was 1∶9 000. The repeatability of the optimal experimental conditions was good. The results of receiver operating characteristic(ROC) curve analysis showed that under the optimal experimental conditions, the area under the ROC curve(AUC) of the detection method was 0.978(95%CI: 0.947～1.000, P=0.000), and the cut-off value was 0.484, and the corresponding sensitivity was 96.0%, and the specificity was 93.0%. Conclusion　The optimal ELISA experimental conditions for the detection of serum anti-CNN2 antibody is constructed.]]> 2020/6/1 16:44:45 SONG Wei, LIU Ling-long, WANG Peng-fei, et al. 62true ［Abstract］　Objective　To explore the risk factors of hypertensive disorder complicating pregnancy(HDCP) and to provide a theoretical basis for prevention of HDCP. Methods　Nine hundred and eighty pregnant women who gave birth in the obstetrics department of our hospital from June 2006 to June 2017 were selected as the research subjects. They were divided into HDCP group(n=141) and non-HDCP group(n=839) according to whether or not HDCP occurred. Based on the hospital′s medical large data platform, the general demographic characteristics, fertility history, family history, disease history, and prenatal blood calcium levels of the study subjects were collected. Logistic regression was used to analyze the risk factors of HDCP. Results　The incidence of HDCP was 14.39%(141/980). The proportion of the mothers in the HDCP group with age ≥35 years, the body mass index(BMI) during pregnancy ≥24.0 kg/m², and the mothers(or their sisters) having a history of hypertension and low blood calcium levels was larger than that in the non-HDCP group, and the difference was statistically significant between the two groups(P<0.05). Logistic regression analysis showed that pregnant women with BMI ≥24.0 kg/m², mothers(or their sisters) with a history of hypertension and hypocalcemia were the risk factors of HDCP in pregnant women. Conclusion　BMI, a family history of hypertension and decreased blood calcium level are closely related to the occurrence of HDCP in pregnant women. The risk identification and management of pregnant women during pregnancy and delivery should be strengthened while the blood calcium level is regularly monitored during pregnancy.]]> 2020/6/1 16:44:45 GUO Ling-ling, YANG Zhi-qing, WANG Hai-xing, et al. 61true ［Abstract］　Objective　To investigate the expression of calcium-binding protein S100A4 in the tissues of pancreatic head carcinoma and its impact on survival and prognosis of pancreatic head carcinoma patients. Methods　Thirty-eight tissue samples of pancreatic head carcinoma resected in our hospital From January 1, 2000 to December 1, 2007 were selected, and 11 normal pancreatic tissues adjacent to the cancer were taken as controls. The expression level of S100A4 protein in the tissue samples was detected by immunohistochemistry. The relationship between the expression level of S100A4 protein and the clinicopathological features of pancreatic head carcinoma and its influence on survival and prognosis of the patients were analyzed. Results　The positive expression rate of S100A4 protein in the pancreatic head carcinoma tissues was 71.05%(27/38), while no positive expression of S100A4 protein was found in the normal pancreatic tissues adjacent to the cancer, and the positive rate was significantly different between the two groups(χ² =14.654, P=0.000). There were significant differences between the S100A4 protein-positive group and the S100A4 protein- negative group of pancreatic head carcinoma tissues in tumor diameter, whether distant metastasis, TNM stage and with or without lymph node metastasis(P<0.05). The survival prognosis of the patients with pancreatic head carcinoma in the S100A4 protein-negative group was significantly better than that in the S100A4 protein-positive group(P<0.05). The results of Cox regression analysis showed that TNM stageⅠand TNM stage Ⅱ were the protective factors of survival prognosis for the patients when TNM stage Ⅳ was taken as reference(P<0.05), and S100A4 positive expression was a risk factor of prognosis for the patients with pancreatic head carcinoma(P<0.05). Conclusion　The elevated expression of S100A4 protein in pancreatic head carcinoma tissues is closely related to the occurrence, development and prognosis of pancreatic head carcinoma.]]> 2020/6/1 16:44:45 JIA Fu-xin, LIU Meng-meng, LIU Jiang-wei 60true ［Abstract］　Objective　To analyze the clinical efficacy of anatomical liver resection(AR) in treatment of hepatocellular carcinoma under the concept of precise liver surgery. Methods　A retrospective study was performed on 206 hepatocellular carcinoma patients who were admitted to the No.923 hospital of people′s liberation army from May 2012 to May 2017 and their clinical data were retrospective analyzed. According to the concept of precise liver surgery, all the patients received careful and complete examination and accurate evaluation of liver reserve function before surgery, and the precise planning of the anatomical hepatectomy scheme, precise operation to complete the corresponding liver sub-segments, liver segments, liver lobes and other AR were conducted through accurate imaging evaluation of the anatomical characteristics of the foci. The operation related indicators were observed and the follow-up was done to evaluate the curative effects. The patients were followed up by telephone after operation and outpatient reexaminations were conducted on them. The follow-up time was up to May 31, 2019. Results　One hundred and seventy-seven cases underwent open hepatectomy and 29 cases underwent laparoscopic hepatectomy. Among the 177 patients, 32 cases were blocked hepatic portal blood flow by Pringle method; 81 cases were blocked by hemi-hepatic blood flow method; 63 cases were blocked hepatic blood flow by selective hepatic lobe or hepatic segment method and the other 30 cases were not blocked hepatic blood flow. The operation time was 45～300(173.8±65.2)min. The amount of intraoperative bleeding was 50～1 000(207.9±142.1)ml. 10 cases received intraoperative blood transfusion among whom 3 cases received intraoperative blood transfusion of red blood cells. The transfusion rate was 4.9% and the average transfusion volume was 235 ml. The postoperative hospital stay was (11.3±2.3)days. After operation, 2 cases were found with liver dysfunction, which was discharged smoothly after medical treatment. There were no perioperative deaths. All the 206 patients were followed up for 24 to 84 months. The 1-, 3-, 5-year survival rates were 85.4%, 57.4% and 39.2% respectively. The 1-, 3-, 5-year tumor-free survival rates were 75.2%, 43.8% and 24.3% respectively. Conclusion　AR is safe and reliable. The short-term and long-term survival effects after surgery are satisfactory. AR can be the first choice for routine surgical treatment of hepatocellular carcinoma.]]> 2020/6/1 16:44:45 PANG Fei-xiong, LAI Yan-hua, HUANG Xiao-chun, et al. 59true ［Abstract］　Objective　To investigate the efficacy of proprioceptive neuromuscular facilitation(PNF) combined with comprehensive therapy of Chinese medicine in rehabilitation for the patients with spinal cord injury. Methods　One hundred and forty-one patients with spinal cord injury and incomplete paralysis who were treated in our hospital from October 2012 to March 2015 were selected and were randomly divided into group A(n=42, treated with PNF plus Chinese medicine comprehensive rehabilitation), group B(n=43, treated with PNF rehabilitation alone) and group C(n=43, treated with Chinese medicine comprehensive rehabilitation alone). The sensory scores, motor scores, and Barthel index scores were compared among the three groups before and after rehabilitation. Results　There were no significant differences in the sensory scores, motor scores and Barthel index scores among the three groups before rehabilitation(P>0.05). The sensory scores, motor scores and Barthel index scores in the three groups after rehabilitation were significantly elevated compared with those before rehabilitation(P<0.05). The scores of the three indicators in group A were significantly higher than those in group B and group C(P<0.05) at the end of rehabilitation treatment, the third month after rehabilitation treatment and the sixth month after rehabilitation treatment. However, there were no significant differences in the scores of the three indicators between group B and group C(P>0.05). Conclusion　PNF combined with Chinese medicine comprehensive rehabilitation is more effective than PNF rehabilitation alone and Chinese medicine comprehensive rehabilitation alone in rehabilitation for the patients with spinal cord injury and incomplete paralysis, which is worthy of clinical application.]]> 2020/6/1 16:44:45 CHU Ye, LIANG Bin, LYU Ya-nan, et al. 58true ［Abstract］　Objective　To compare the therapeutic effects of simple phacoemulsification and phacoemulsification combined with goniosynechialysis in treatment of primary acute angle-closure glaucoma with cataract. Methods　The clinical data of 110 patients(110 eyes) with primary acute angle-closure glaucoma complicated with cataract who were admitted to our hospital from February 2017 to August 2018 were analyzed retrospectively. According to different treatment methods, the patients were divided into simple phacoemulsification group(group A) and phacoemulsification combined with goniosynechialysis group(group B), with 55 cases in each group. Intraocular lenses were implanted in both groups. The best corrected visual acuity(BCVA), intraocular pressure, opening degree of the anterior chamber angle and complications were compared between the two groups. Results　Compared with those before surgery, the BCVA and intraocular pressure in the two groups were significantly improved 1 week and 6 months after surgery(P<0.05), but there were no significant differences between the two groups(P>0.05). Compared with that before surgery, the opening degree of the anterior chamber angle in the two groups was significantly higher after surgery(P<0.05), and the opening degree of the anterior chamber angle in group B was slightly higher that in group A, with no significant difference between the two groups(P>0.05). There was no significant difference in the incidence of postoperative complications between the two groups(P>0.05). Conclusion　Simple phacoemulsification and phacoemulsification combined with goniosynechialysis can make the intraocular pressure in the patients with primary acute angle-closure glaucoma complicated with cataract get good control in the short term and in the medium and long term, with good clinical effects.]]> 2020/6/1 16:44:45 GUO Xiu-juan, CHEN Di, LU Hao-quan, et al. 57true ［Abstract］　Objective　To analyze the pathological characteristics of asymptomatic hematuria in children, and to explore the indications of renal biopsy in children with asymptomatic hematuria. Methods　The pathological and clinical data of 204 children with asymptomatic hematuria who underwent renal biopsy in our hospital from January 2010 to January 2019 were retrospectively analyzed, and the patients were divided into different groups according to the degree of hematuria, complications of proteinuria and age. The general clinical features, immunoglobulin levels and pathological types were compared among groups. Results　The proportion of males, age, duration of disease, immunoglobulin A(IgA) level, IgA nephropathy(IgAN), proportion of glomerular minor lesion(GML), and proportion of the patients without glomeruli were statistically different among the simple hematuria group, the hematuria accompanied with microproteinuria group, and the hematuria accompanied with medium proteinuria group(P<0.05). The proportion of left renal vein compression syndrome in the gross hematuria group was significantly higher than that in the microscopic hematuria group(P<0.05). The quantitative levels of 24-hour urine protein and the proportion of GML were significantly different among the different age groups(P<0.05). Conclusion　Simple hematuria is not recommended as an indicator of early renal biopsy in children, but renal biopsy can be performed in time for asymptomatic hematuria children with a family history of renal disease or proteinuria to make early diagnosis.]]> 2020/6/1 16:44:45 XIE Dan-feng, YANG Hai-ping 56true ［Abstract］　Objective　To investigate the correlation between the drug-resistant rate of Staphylococcus aureus and the consumption of antibacterial agents, and to provide a reference for the rational use of antibacterial agents in clinical practice. Methods　A retrospective analysis of the frequency of use of eight types of antibacterial agents and the drug-resistant rate of Staphylococcus aureus was performed in our hospital from 2014 to 2018, and the correlation between the antibacterial drug cumulative defined daily doses(DDDs) and the drug-resistant rate of Staphylococcus aureus was analyzed using Pearson correlation analysis. Results　The penicillin resistance rate of Staphylococcus aureus was positively correlated with DDDs of the third-generation cephalosporins(r=0.912) and quinolones(r=0.940) antibacterials(P<0.05). The rate of oxacillin resistance was positively correlated with DDDs of the compound preparations containing enzyme inhibitors(r=0.902, P<0.05). The resistance rate of Staphylococcus aureus to levofloxacin was highly positively correlated with DDDs of the first-generation cephalosporins(r=0.949) and macrolides(r=0.861) antibacterials(P<0.05), and was highly negatively correlated with DDDs of the compound preparations containing enzyme inhibitors(r=-0.852, P<0.05). The clindamycin resistance rate were highly positively correlated with DDDs of the first-generation cephalosporins(r=0.921, P<0.05). Conclusion　There is a certain correlation and complex relationship between DDDs of antibacterial agents and drug-resistance rates of Staphylococcus aureus.]]> 2020/6/1 16:44:45 PENG Fang, SUN Fu-dong, YE Xin-min, et al. 55true ［Abstract］　Objective　To analyze the relationship between visceral obesity and the effects of laparoscopic colorectal cancer radical operation and the patients′ prognosis. Methods　One hundred and three patients with colorectal cancer who underwent radical laparoscopic colorectal cancer surgery in our hospital between February 2012 and October 2013 were selected. The research subjects were divided into the visceral obesity group(SMA angle ≥51.75°, n=52) and the non-visceral obesity group(SMA angle <51.75°, n=51) according to different SMA angles. The correlation between visceral obesity and the operation time, intraoperative blood loss, rate of converting to laparotomy, lymph node metastasis rate, postoperative hospital stay, postoperative complications and postoperative survival time was analyzed. Results　The SMA angle could be used to diagnose visceral obesity. The area under the receiver operating characteristic(ROC) curve was 0.771(95%CI: 0.680～0.863, P<0.05), and the cutoff value was 51.75°. The sensitivity was 75.00% and the specificity was 74.50%. The proportion of males aged ≥60 years old in the visceral obesity group was higher than that in the non-visceral obesity group, and the proportion of the patients with lymph node metastasis rate ≥18% in the visceral obesity group was significantly lower than that in the non-visceral obesity group(P<0.05). The operation time of the visceral obesity group was longer than that of the non-visceral obesity group, and the rate of converting to laparotomy was higher than that of the non-visceral obesity group, and the differences were statistically significant(P<0.05). The complication rate was higher in the visceral obesity group than that in the non-visceral obesity group(P<0.05), but there was no significant difference in Clavien-Dindo classification between the two groups(P>0.05). The length of postoperative hospital stay in the visceral obesity group was longer than that in the non-visceral obesity group, and the difference was statistically significant(P<0.05). The median overall survival(OS) of the visceral obesity group was 40 months, and the median OS of the non-visceral obesity group was 38 months. There was a statistically significant difference in the median OS between the two groups(P<0.05). Univariate analysis showed that age, lymph node metastasis, TNM stage, and tumor differentiation were related to OS in the patients with colorectal cancer(P<0.05). Cox regression analysis showed that TNM stage=Ⅲ, high and moderate differentiation of the tumors and visceral obesity were the protective factors of OS after radical resection of colorectal cancer, and lymph node metastasis was a risk factor. Conclusion　SMA angle can be used as a diagnostic indicator of visceral obesity, and the diagnostic efficiency is the highest when the SMA angle is 51.75°. At the same time, visceral obesity can affect the operation of colorectal cancer and increase the incidence of postoperative complications, but it can improve the long-term prognosis and prolong the survival time of the patients.]]> 2020/4/30 17:42:56 CHENG Huang-rong, LI Hua-li, HUANG Xiao-dong, et al. 54true ［Abstract］　Objective　To investigate the expressions of phosphorylation ataxia telangiectasia mutated(p-ATM) protein and checkpoint kinase 2(CHK2) protein in nasal nature killer/T-cell lymphoma(NKTCL) tissues in Guangxi and their clinical significances. Methods　The NKTCL tissues of 49 patients(research group) and their clinical data were collected in our hospital from January 2010 to December 2016, and other 30 cases of nasopharyngeal mucosa benign lymphoid proliferation(NBLP) tissues at the same time were taken as control group. The expressions of p-ATM protein and CHK2 protein in nasal NKTCL and NBLP tissues were compared. The correlation between p-ATM protein and CHK2 protein expression levels in NKTCL tissues and clinical characteristics and prognosis of the patients was analyzed. Results　 In the research group, there were 23 cases(46.94%) with high expression of p-ATM protein and 26 cases(53.06%) with low expression of p-ATM protein. In the research group, there were 24 cases(48.98%) with high expression of CHK2 protein and 25 cases(51.02%) with low expression of CHK2 protein. In the control group, p-ATM protein and CHK2 protein were low expressed, and the difference between the two groups was statistically significant(P<0.05). In the nasal NKTCL tissues,the ratio of clinical stage Ⅰ/Ⅱ in the CHK2 high expression group was lower than that in the CHK2 low expression group(P<0.05). Kaplan-Meier analysis showed that the median survival time of the high expression group of p-ATM in the nasal NKTCL tissues was 6 months, and the median survival time of the p-ATM low expression group was 21 months, and the survival prognosis of the low expression group was better than that of the high expression group(P=0.011). The median survival time of CHK2 high expression group in the nasal NKTCL tissues was 7 months, and the median survival time of the low expression group was 21 months, and the survival prognosis of the low expression group was better than that of the high expression group(P=0.025). There was a positive correlation between p-ATM protein expression and CHK2 protein expression in the nasal NKTCL tissues(P<0.05). Conclusion　The abnormal expressions of ATM and CHK2 proteins play an important role in the occurrence and development of nasal NKTCL in Guangxi. Detection of p-ATM and CHK2 protein expression levels in nasal NKTCL tissues is helpful to assess the prognosis of the patients.]]> 2020/4/30 17:42:56 MO Xiang-lan, YIN Wu, HUANG Yong-ta, et al. 53true ［Abstract］　Objective　To investigate the correlation between down-regulation of cadherin-13(CDH13) expression and the growth of bladder cancer, and its effects on phosphatidylinositol 3 kinase(PI3K)/phosphorylated protein kinase B(p-Akt)signal pathways. Methods　Nude mouse tumor formation models were established using bladder cancer 5637 cells, and tumor volume was compared among the normal control group(5637 cells treated with 1×PBS, n=7), the negative control group(transfected 5637 cells with shRNA plasmid, n=7) and the experimental group(transfected 5637 cells with CDH13 shRNA plasmid, n=7) at different time points. Western blot was used to detect the expressions of CDH13, PI3K and p-Akt proteins in the tumor tissues, and the expressions of the three proteins were compared among the three groups. Results　During the experimental period, the tumor volume showed an increasing trend in the nude mice of the three groups, and the tumor volume of the experimental group increased fastest, and the change magnitude was statistically significant among the three groups(P<0.05). On the 10th day, the 13th day, and the 16th day, the tumor volume of the nude mice in the experimental group was significantly larger than that in the negative control group and the normal control group(P<0.05), while the tumor volume of the negative control group was significantly larger than that of the normal control group(P<0.05). Western blot results showed that the level of CDH13 expression in the experimental group was significantly lower than that in the negative control group and the normal control group(P<0.05), while the expression levels of PI3K and p-Akt proteins in the experimental group were significantly higher than those in the negative control group and the normal control group(P<0.05). Conclusion　Down-regulation of CDH13 expression may promote the occurrence of bladder cancer through the PI3K / p-Akt signaling pathways.]]> 2020/4/30 17:42:56 LIN Ying-li, LI Yan-li, LIANG Jie, et al. 52true ［Abstract］　Objective　To explore the influencing factors of clinical pregnancy outcomes in advanced age women undergoing in vitro fertilization or intracytoplasmic sperm injection(IVF/ICSI), and to provide rational suggestions for the patients. Methods　A retrospective analysis of 633 cycles of IVF / ICSI treatment in the reproductive center of a class A tertiary hospital in Nanning city from January 2016 to December 2018 was performed. According to the clinical pregnancy outcomes, the patients were divided into the successful conception group(182 cycles) and the unsuccessful conception group(451 cycles). The influencing factors of the pregnancy outcomes were analyzed by multivariate Logistic regression analysis. Results　The pregnancy rates of older women(OR=0.682, 95%CI=0.604～0.770), those with cesarean section history(OR=0.411, 95%CI=0.232～0.729) and those with dysmenorrhea history(OR=0.257, 95%CI=0.074～0.893) were decreased. The higher the number of antral follicles(OR=1.052, 95%CI=1.010～1.096) and the higher the quality of embryo transfered(OR=1.310, 95%CI=1.032～1.664), the higher the pregnancy rate. Conclusion　The majority of women should reasonably plan their childbearing age to prevent the occurrence of infertility in advanced age from their own. Assisted reproductive institutions should make a detailed and comprehensive assessment of the health status and fertility of advanced age women, and give objective and sufficient information on the fertility rate and risks, so as to make safe and reasonable treatment protocols for the advanced age women in all aspects.]]> 2020/4/30 17:42:56 HUANG Tai-shuai, ZUO Yan-li, TENG Min, et al. 51true ［Abstract］　Objective　To investigate the therapeutic effect of kinesiology taping combined with manual lymphatic drainage on patients with shoulder-hand syndrome after stroke and provide theoretical basis for clinical treatment. Methods　Fifty-seven patients with shoulder-hand syndrome after stroke in our hospital from April 2018 to July 2019 were selected and randomly divided into control group(27 cases) and observation group(30 cases). The control group was treated with manual lymphatic drainage therapy on the basis of conventional rehabilitation training, and the observation group was treated with the same treatment as the control group plus kinesiology taping. The Visual Analogue Scale(VAS) scores, volume difference of both hands, and Fugl-Meyer Index of Upper Extremity(FMA-UE) scores were compared between the two groups before and after treatment. Results　Before treatment, there were no significant differences in the VAS scores, volume difference of both hands and the FMA-UE scores between the two groups(P>0.05). After treatment, the VAS scores and volume difference of both hands in the observation group were significantly lower than those in the control group(P<0.05), and the FMA-UE scores in the observation group were significantly higher than those in the control group(P<0.05). Conclusion　Kinesiology taping combined with manual lymphatic drainage can relieve the swelling and pain of the patients with shoulder-hand syndrome after stroke, and promote the recovery of upper limb function in the patients, which is worthy of clinical application.]]> 2020/4/30 17:42:56 XU Pei-dong, HE Xing-jin, YANG Huan, et al. 50true ［Abstract］　Objective　To explore the application value of metagenomic next-generation sequencing(mNGS) in detection of pathogens in patients with severe pneumonia. Methods　Twenty-six patients with severe pneumonia were enrolled from July 2018 to May 2019. The diagnostic efficacies of the mNGS and the traditional detection method on the pathogenic bacteria were compared, and the impact of antibiotic exposure on the detection rate was evaluated. Results　The positive rate of pathogenic bacteria detected by mNGS(92.3%) was significantly higher than that detected by the traditional culture method(42.3%)(P<0.05). Under the premise of using antibiotics, the positive rate of pathogenic bacteria detected by mNGS(91.3%) was significantly higher than that detected by the traditional method(43.5%)(P<0.05). Conclusion　mNGS has a high sensitivity to the identification of pathogenic bacteria, and the use of antibiotics has little effect on the detection results of mNGS, which has an important clinical application value for the diagnosis and treatment of infected patients.]]> 2020/4/30 17:42:57 PAN Chun-xi, LYU Li-wen 49true ［Abstract］　Objective　To compare the clinical characteristics of idiopathic membranous nephropathy(IMN) with atypical membranous nephropathy(AMN), focal segmental glomerulosclerosis(FSGS), minimal change nephropathy(MCN) and mild mesangial proliferative nephropathy(MMPN). Methods　A total of 411 kidney disease patients who were treated in a research unit from June 2013 to January 2018 were selected, including 245 patients with IMN, 37 patients with AMN, 36 patients with FSGS, 51 patients with MCN, and 42 patients with MMPN. The gender, age, incidence of hypertension, incidence of nephrotic syndrome, incidence of renal dysfunction, and the levels of 24-hour urine protein, albumin, uric acid, total cholesterol(TC), triglyceride(TG), low density lipoprotein(LDL) and high density lipoprotein(HDL) were compared among the five groups. Results　The age of the IMN group was significantly higher than that of the FSGS group, the MCN group and the MMPN group(P<0.05). The incidence of hypertension in the IMN group was significantly higher than that in the MCN group(P<0.05), but the incidence of nephrotic syndrome in the IMN group was significantly lower than that in the MCN group(P<0.05). The incidence of renal dysfunction in the IMN group was significantly lower than that in the FSGS group(P<0.05). The level of albumin in the IMN group was significantly higher than that in the FSGS group, the MMPN group and the MCN group(P<0.05). The level of uric acid in the IMN group was significantly higher than that in the AMN group, but lower than that in the FSGS group(P<0.05). The level of TC in the IMN group was significantly lower than that in the MCN group, the FSGS group and the MMPN group(P<0.05). The levels of LDL and HDL in the IMN group were significantly lower than those in the MCN group and the FSGS group(P<0.05). Conclusion　There are differences in the clinical characteristics of IMN, AMN, FSGS, MCN and MMPN. In the course of treatment, clinicians should identify the differences to improve the treatment plan.]]> 2020/4/30 17:42:57 PAN Qing-Deng, YE Zu-Lan, ZENG Chun, et al. 48true ［Abstract］　Objective　To investigate the expression of PFTAIRE protein kinase 1(PFTK1) in rectal cancer and its correlation with the clinical characteristics of rectal cancer patients. Methods　Fifty-six rectal cancer tissue samples which were surgically resected in our hospital from January 2017 to August 2018 were selected, and 56 cases of matched paracancerous tissue samples were selected. Immunohistochemical staining was used to detect the expression of PFTK1 in the samples, and the correlation between PFTK1 expression in rectal cancer tissues and clinical characteristics of the patients was analyzed. Results　Forty cases(71.43%) had high expression of PFTK1 in the rectal cancer tissues, and 18 cases(32.14%) in the paracancerous tissues, with a significant difference between the two tissues(P<0.05). There were significant differences in the distributions of clinical staging, N staging and T staging between the PFTK1 high expression group(n=40) and the PFTK1 low expression group(n=16) (P<0.05). In addition, the incidence of lymph node metastasis and preoperative carcino-embryonic antigen(CEA) >5 ng/ml was higher in the high expression group than that in the low expression group, and the difference was statistically significant(P<0.05). Conclusion　The expression of PFTK1 in human rectal cancer is elevated, and the high expression of PFTK1 is correlated with the clinical stage, N staging, T staging and lymph node metastasis of the patients with rectal cancer and preoperative CEA level.]]> 2020/4/10 18:04:33 ZHANG Yu-bo, WAN Sheng-yun, QIAN Bo, et al. 47true ［Abstract］　Objective　To investigate the application effect of enhanced recovery after surgery(ERAS) on elderly patients undergoing total hip arthroplasty(THA). Methods　The data of 80 elderly patients who underwent THA in our hospital from January 2017 to January 2019 were retrospectively analyzed. Among the elderly patients, 40 cases receiving ERAS concept-guided treatment as the ERAS group, and the other 40 cases receiving the traditional treatment plan as the control group. The hip joint function Harris scores, Visual Analogue Pain Scale(VAS) scores, postoperative complication rate, postoperative hospital stay, hospitalization costs and the patients′ satisfaction rate were compared between the two groups. Results　The average intraoperative blood loss was (203.88±54.92)ml in the ERAS group, and (386.75±131.82)ml in the control group, and the difference was statistically significant(P<0.05). The incidence of postoperative complications was 5.00%(2/40) in the ERAS group and 35.00%(14/40) in the control group，and the difference was statistically significant(P<0.05). The postoperative VAS scores in the ERAS group was decreased significantly compared with those in the control group(P<0.05), while the postoperative hip joint Harris scores in the ERAS group was increased significantly compared with those in the control group(P<0.05). The postoperative hospitalization time of the ERAS group was significantly shorter than that of the control group, and the hospitalization costs of the ERAS group were significantly lower than those of the control group. The satisfaction rate of the ERAS group was significantly higher than that of the control group(P<0.05). Conclusion　The recovery of hip joint function is faster and better in elderly patients after THA under the guidance of ERAS, and the improvement of out-of-hospital guidance can reduce the re-admission rate of the patients.]]> 2020/4/10 18:04:33 OUYANG Peng-hui, HUANG Yu, LIU Wen-hui, et al. 46true ［Abstract］　Objective　To investigate the sleep status of asthmatic children during the periods of attack and remission. Methods　Fifty children with asthma attack who were treated in our hospital from July 2018 to August 2019 were selected as the study subjects, and all the subjects entered into remission after receiving standardized treatment. The lung ventilatory function test, asthma control score, and Sleep Disturbances Scale for Children(SDSC) scores were analyzed and compared between the children with asthma attack and those with asthma remission. Results　The incidence of sleep disorders in the children with asthma attack was significantly higher than that in the children with asthma remission(92.00% vs 68.00%, P<0.05). Compared with the children with asthma attack, the children with asthma remission had significantly lower six dimension scores in SDSC and lower total sleep score(P<0.05). Compared with the children with asthma attack, the children with asthma remission had significantly longer total sleep time in the nighttime and shorter time to fall asleep after going to bed at night(P<0.05). The abnormal sleep at night in the children with asthma attack was more serious than that in the children with asthma remission. Conclusion　Children with asthma may have more sleep disorders, especially during asthma attacks.]]> 2020/4/10 18:04:33 LIU Shu-jun, XIE Qing-ling, QIN Ling, et al. 45true ［Abstract］　Objective　To study the correlation between serum levels of vitamin A, D, E and anemia during pregnancy. Methods　One thousand pregnant women receiving routine examination of parturient in Xuchang Central Hospital were selected during January 2017 and January 2019 and were divided into anemia group(n=600, HGB<100 g/L) and non-anemia group(n=400, HGB≥100 g/L) according to their different levels of hemoglobin(HGB). The three items of anemia(including ferritin, folic acid and vitamin B₁₂), blood routine indexes［HGB, mean corpuscular volume(MCV) and hematocrit(HCT)］, the serum levels of vitamin A, D and E were compared between the two groups. The abnormal levels of serum vitamin A, D and E(deficiency rate and excessive rate) were compared between the two groups. The correlation between serum levels of vitamin A, vitamin D and vitamin E and three indexes of anemia and blood routine indexes was analyzed. The follow-up results of the anemia group were analyzed. Results　The levels of ferritin, folic acid, vitamin B₁₂, HGB and HCT in the anemia group were significantly lower than those in the non-anemia group(P<0.05). The serum levels of vitamin A and vitamin D in the anemia group were significantly lower than those in the non-anemia group(P<0.05). The deficiency rates of vitamin A and vitamin D in the anemia group were significantly higher than those in the non-anemia group(P<0.05). There were no significant differences in the distributions of vitamin E deficiency and vitamin E excess between the two groups(P>0.05). The serum levels of vitamin A and vitamin D were positively correlated with ferritin and HGB(P<0.05). There was no significant correlation between serum vitamin A, vitamin D, vitamin E and folic acid, vitamin B₁₂, MCV, HCT(P>0.05). In the anemia group, the rational uses of vitamins and dietary adjustment were given, and the HGB of 516 cases recovered to ≥100 g/L before delivery. Conclusion　Serum vitamin A and vitamin D are positively correlated with ferritin and HGB. The level of vitamin E is less associated with anemia during pregnancy.]]> 2020/4/10 18:04:33 ZHOU Li-juan, GONG Jun 44true ［Abstract］　Objective　To explore a rural chronic disease management model based on three-dimensional multipartite cooperation and evaluate its implementation effect. Methods　In 2013, a chronic disease management team was established based on Xiaochang′an Town Health Center in Hechi City to improve the health management plan for chronic diseases, to coordinate and organize the village doctors to carry out the chronic disease management business, and to innovate the rural chronic disease management mode with the help of the Chronic Disease Prevention and Treatment Hospital of Futian District in Shenzhen City and the support of the village committee. Results　From 2014 to 2018, the health management level and management effect of chronic diseases in Xiaochang′an Town were significantly improved compared with those before 2014. The number of the residents who were established health files and were incorporated management in the town increased from 17 899 to 25 040, and the filing rate increased by 23.20%. The number of the residents receiving standardized managements of hypertension and diabetes increased from 1 201 and 156 in 2014 to 2 158 and 238 in 2018, respectively. The normative management rates of hypertension and diabetes increased by 10.87% and 1.15% respectively. The normative management rates of blood pressure and blood sugar reaching standard increased by 25.29% and 26.33%, and the standard management satisfaction rates increased by 21.38% and 29.77%, respectively. Conclusion　The multipartite collaborative three-dimensional rural chronic disease management model established in Xiaochang′an Town has achieved satisfactory results in the disease control of diabetes and hypertension, and is worth promoting in the management of chronic diseases in rural areas in China.]]> 2020/4/10 18:04:33 ZHOU Zhi-heng, LIU Xiu-juan, WEN Sheng-chun, et al. 43true ［Abstract］　Objective　To explore the clinical efficacy and safety of prolonged placement of balloon uterine stent in prevention of readhension after the separation of moderate to severe intrauterine adhesions. Methods　A total of 210 patients diagnosed with moderate to severe intrauterine adhesions and underwent adhesion separation were selected in our hospital from January 2016 to December 2018. The patients were divided into control group and observation group by random number table method, with 105 cases in each group. In the control group, the balloon uterine stent was placed after the separation of intrauterine adhesions by hysteroscopy, and were removed one week later. In the observation group, the balloon uterine stent was placed after the separation of intrauterine adhesions by hysteroscopy, and the tail tube of the balloons was cut to let out the fluid in the balloons one week later, and the stents of the balloons were retained. After the second menstruation, they were removed. The incidence rates of postoperative intrauterine adhesions, menstrual recovery and complications were compared between the two groups. Results　The improvement rate of the first menstrual period after operation was 72.38% in the control group and 77.14% in the observation group, but the difference was not statistically significant(P>0.05). However, at the 2nd month, 3rd month and 6th month after operation, the improvement rates of menstruation in the observation group were significantly higher than those in the control group(P<0.05). The effective rate of improvement of intrauterine adhesions was 76.19% in the control group and 87.62% in the observation group, with a significant difference between the two groups(P<0.05). The incidence of postoperative complications in the observation group was higher than that in the control group, and the difference was statistically significant(9.52% vs 0.00%, χ²=10.500, P=0.001). Conclusion　Prolonged placement of balloon uterine stent can improve intrauterine adhesion and menstruation. Although prolonged placement of balloon uterine stent has certain risks, the complications caused by the balloon placement can be cured after treatment and is relatively safe. Clinical work still requires close observation and follow-up.]]> 2020/4/10 0:00:00 WEI Hong-lan, LIN Zhong, FAN Hui-liu, et al. 42true ［Abstract］　Objective　To explore the clinical characteristics and preventive measures of postoperative hemorrhage of secondary hyperparathyroidism(SHPT). Methods　One hundred SHPT patients were performed operation in our hospital from August 2014 to August 2017, among whom 6 cases had postoperative bleeding and their clinical data were retrospectively analyzed. Results　Of the 6 cases, 2 cases had skin flap bleeding, 2 cases had anterior cervical vein hemorrhage and 2 cases had anterior cervical muscle bleeding. The 6 cases were given corresponding treatment measures. No severe consequences such as asphyxia occurred and they were cured and discharged. Conclusion　Dealing with all the causes of perioperative bleeding and operating on the patients carefully during the operation and completing hemostasis before closing the incision are the key points to avoid bleeding after operation.]]> 2020/4/10 18:04:33 LIAO Dan, ZHANG Meng-die, ZHENG Hou-pu, et al. 41true ［Abstract］　Objective　To establish the colorectal cancer cell model resistant to concurrent chemoradiotherapy in vitro and explore the changes of messenger ribonucleic acid(mRNA) expression profile. Methods　HCT116 colorectal cancer radiation resistance cell(HCT116 CRR) was obtained by simulating clinical high-dose therapy. mRNA microarray was used to compare the mRNA expression profiles of model cells and wild-type cells, and to preliminarily screen the mRNA related to concurrent chemoradiotherapy resistance in colorectal cancer. Results　The HCT116 CRR cell line was successfully constructed. Compared with the wild-type HCT116 cells, there were 3 832(13.88%) mRNAs with a difference of more than 2 times, of which 1 847 mRNAs were more than 2 times up-regulated, 1 985 mRNAs were more than 2 times down-regulated, 35 mRNAs were more than 10 times up-regulated and 50 mRNAs were more than 10 times down-regulated using mRNA microarray. Conclusion　Compared with its wild-type HCT116 cells, the HCT116 CRR cell line has a significant change of mRNA expression profile. The differentially expressed mRNA may be involved in the molecular regulation of radiotherapy and chemotherapy resistance in colorectal cancer.]]> 2020/3/16 15:42:27 LIU Shan, SHEN Hong-mei, LIU Xin-yuan, et al. 40true ［Abstract］　Objective　To observe the clinical efficacy of magnesium sulfate in treatment of early severe hand, foot and mouth disease(HFMD) in children. Methods　Ninety-two children with early severe HFMD in our hospital were selected and were divided into control group(n=31) and observation group(n=61) according to different treatment methods. The control group was given routine symptomatic treatment while the observation group received the same treatment as the control group plus magnesium sulfate. The clinical efficacy and laboratory indicators were observed in the two groups before and after treatment. Results　In the observation group, 40 cases were cured, 18 cases effective and 3 cases ineffective. In the control group, 14 cases were cured, 11 cases effective and 6 cases ineffective. The clinical efficacy of the observation group was better than that of the control group(P<0.05). After treatment, the concentrations of serum zinc and calcium ions in the two groups were significantly increased compared with those before treatment(P<0.05), and the concentration of magnesium ion in the observation group was significantly higher than that in the control group(P<0.05). After treatment, the levels of serum neuron-specific enolase(NSE), norepinephrine(NE), dopamine(DA), creatine kinase isoenzyme(CK-MB) and interferon-γ(IFN-γ) in the observation group were significantly decreased compared with those before treatment(P<0.05) while the level of serum cystatin C(CysC) was significantly increased compared with that before treatment(P<0.05). In the control group, only the level of NSE was significantly decreased compared with that before treatment and the level of CysC was significantly increased compared with that before treatment(P<0.05). After treatment, there were significant differences in the levels of NSE, CysC, NE, DA, CK-MB and IFN-γ between the two groups(P<0.05). The duration of neurological involvement and the time for leukocytes and blood glucose to return to normal in the observation group was significantly shorter than that in the control group(P<0.05). The total rate of complications in the observation group was lower than that in the control group(4.92% vs 19.35%), but there was no significant difference between the two groups(P>0.05). Conclusion　Magnesium sulfate can effectively improve the clinical efficacy of early severe HFMD in children, improve the symptoms of neurological involvement and side effects of hormone therapy, and is safe and effectively reduces the complications such as meningitis and pulmonary edema.]]> 2020/3/16 15:42:27 CHEN Fang, SONG Chun-lan, GU Xue, et al. 39true ［Abstract］　Objective　To summary the process and experience of limb salvage treatment of 10 patients with severe injury of lower extremities. Methods　Retrospective analysis the clinical data of 10 patients with severe injury of lower extremities were summarized from June 2018 to May 2019. All the patients underwent debridement and suture, external fixation and stent fixation, neurovascular tendon anastomosis and vacuum sealing drainage(VSD) treatments within 2 to 10 hours after injury. Results　The affected limbs of 7 patients were preserved among whom 5 cases could walk on their own, with satisfactory functional evaluation, and the other 2 cases had poor functional recovery after surgery, walking with a claudication and obvious pain. Two patients suffered from ischemic necrosis accompanied by infection and underwent second stage debridement and amputation of lower thighs. One patient received lower limb stump repair during srugery because the lower extremities were damaged. Conclusion　Correct assessment of the damage degree of the affected lower limbs and reasonable formulation of operation plans can improve the effectiveness of limb preservation, reduce the disability rate and reduce the psychological and economic burden of the patients.]]> 2020/3/16 15:42:27 XU Qian, ZHANG Xia, LIANG Zhi-bai, et al. 38true ［Abstract］　Objective　To compare the therapeutic effects of zoledronic acid and salmon calcitonin on osteoporosis after percutaneous kyphoplasty(PKP). Methods　One hundred and two patients with osteoporotic vertebral compression fractures were selected in the Second Affiliated Hospital of Zhengzhou University and were randomly divided into zoledronic acid group(n=52) and salmon calcitonin group(n=50). The zoledronic acid group receiving PKP plus zoledronic acid treatment and the salmon calcitonin group receiving PKP plus salmon calcitonin treatment. The vertebral Bone Mineral Density(BMD) T score, Visual Analogue Scale(VAS) scores, Oswestry Disability Index(ODI) score, vertebral height, incidence of adjacent vertebral refractures and related complications were compared between the two groups. Results　Compared with those before operation, the vertebral BMD T scores were higher after operation in both of the two groups. The vertebral BMD T scores in the zoledronic acid group 6,12, 24 months after operation were significantly higher than those in the salmon calcitonin group(P<0.05). Compared with those before operation, the VAS scores and ODI scores were improved after operation in both of the two groups. The improvements of the salmon calcitonin group 1, 3, 6 months after treatment were better than those of the zoledronic acid group(P<0.05). The postoperative vertebral height in the two groups recovered significantly(P<0.05), however, there was no significant difference between the two groups(P>0.05). During the treatment period, there were no significant differences in the incidence rates of adjacent vertebral refractures and the other related complications between the two groups(P>0.05). Conclusion　Zoledronic acid has better anti-osteoporosis effect and salmon calcitonin has better analgesic effect on patients with osteoporosis after PKP.]]> 2020/3/16 15:42:27 WANG Yuan-ji, ZHANG Ming-sheng, ZHU Yi-fei, et al. 37true ［Abstract］　Objective　To explore the efficacy and safety of estradiol valerate dienogest in treatment of menorrhagia. Methods　Sixty patients with menorrhagia admitted to our hospital from January 2017 to December 2018 were randomly divided into control group and observation group by random number table method, with 30 cases in each group.The control group was treated with estradiol valerate+progesterone, and the observation group was treated with estradiol valerate dienogest. The clinical efficacy, the changes of menstrual volume and hemoglobin, and adverse reactions were compared between the two groups. Results　The effective rate of the observation group(93.33%) was significantly higher than that of the control group(73.33%)(P<0.05). After 3 and 6 months of treatment, the amount of menstruation in the two groups decreased, and the trend of decrease in the observation group was more significant than that in the control group(P<0.05). Compared with those before treatment, the hemoglobin levels of the two groups after treatment increased, and the hemoglobin level of the observation group after treatment was significantly higher than that of the control group(P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups(P>0.05). Conclusion　Estradiol valerate dienogest can effectively treat menorrhagia, reduce the amount of menstrual bleeding and improve the level of hemoglobin.]]> 2020/3/16 15:42:27 YANG Liu, ZHAO Ren-feng, XU Li-li, et al. 36true ［Abstract］　Objective　To observe the effect of dexmedetomidine on pulmonary edema and serological indicators in adult patients with acute respiratory distress syndrome(ARDS) treated with mechanical ventilation through pulse-indicated continuous cardiac output(PiCCO) refined monitoring. Methods　Sixty cases of adult ARDS patients in the Department of Critical Care Medicine of the People′s Hospital of Guangxi Zhuang Autonomous Region were collected and randomly divided into dexmedetomidine group and propofol group by random number table method, with 30 cases in each group. On the basis of sufficient analgesia, the patients were sedated with dexmedetomidine(the dexmedetomidine group) and propofol(the propofol group). All the patients were monitored with PiCCO, and the oxygenation index, extravascular lung water index(EVLWI), pulmonary vascular permeability index(PVPI) and serological indexes C-reaction protein(CRP), procalcitonin(PCT) were compared between the two groups at 0, 24, 48 and 72 hours after sedation. Results　There were no significant differences in age, gender, Sequential Organ Failure Assessment(SOFA) score and initial oxygenation index between the two groups(P>0.05). The target sedative effect was achieved in all the patients. The oxygenation index of the patients in the two groups increased with time, while EVLWI, PVPI, CRP and PCT in the two groups decreased with time, but there were no significant differences between the two groups(P>0.05). Conclusion　Sedation with dexmedetomidine can improve pulmonary edema and systemic inflammatory response in adult patients with ARDS, and the improvement of dexmedetomidine is no less than that of propofol.]]> 2020/3/16 15:42:27 JIANG Ling-yu, HAN Lin, XIONG Bin, et al. 35true ［Abstract］　Objective　To study the practice satisfaction of medical practitioners in Liuzhou public hospitals and its influencing factors. Methods　Three hundred and six medical practitioners were selected from Liuzhou City′s 9 public hospitals at or above the second level as the survey subjects by stratified random sampling method. The questionnaire survey was used to study the practice satisfaction of the medical practitioners and analyze the related factors influencing their satisfaction in practice. Results　In this study, 54.58%(167/306) of the practitioners were satisfied with the current practice environment. When the hospital level, age, gender, education level, department, professional title, working hours per week and employment form were grouped, the difference of satisfaction scores among the groups was statistically significant(P<0.05). The results of binary Logistic regression analysis showed that the overall situation of physician profession would be better, and the overall satisfaction of the practitioners having ten item characteristics including having the idea that they liked the profession very much and that they did not have the idea of changing their profession was higher(P<0.05). Conclusion　The medical practitioners′ professional satisfaction of public hospitals in Liuzhou City of Guangxi is at a general level. It is necessary to strengthen the optimal allocation of medical resources, implement the hierarchical diagnosis and treatment system, improve the salary system and create a harmonious working environment to improve the professional satisfaction of medical staff.]]> 2020/3/16 15:42:27 LIU Jin-ji, CHEN Qiu-ju, MO Xiong-jun, et al. 34true ［Abstract］　Objective　To explore the relationship between the types of left ventricular false tendons(LVFT) and frequent ventricular premature beats. Methods　One hundred and forty-nine patients with LVFT who were detected by echocardiography in our hospital from January 2016 to June 2018 were enrolled in this study. According to whether they were complicated with frequent ventricular premature beats, the patients were divided into group A(LVFT complicated with frequent ventricular premature beats group, n=28) and group B(LVFT without frequent ventricular premature beats group, n=121). The general clinical data of the patients in the two groups were collected, and two-dimensional echocardiography was used to observe and record the thickness, connection modes and shapes of the LVFT. The influencing factors of frequent ventricular premature beats were analyzed by binary Logistic regression. Results　With LVFT thickness type 1 as the reference, LVFT thickness type 3 was a risk factor for frequent ventricular premature beats(OR=4.456, P=0.033). With LVFT connection type 1 as the reference, LVFT connection type 2 was a risk factor for frequent ventricular premature beats(OR=12.594, P=0.024). With LVFT shape type 1 as the reference, LVFT shape type 2 was a inhibiting factor for frequent ventricular premature beats(OR=0.185, P=0.002). Longer course of palpitations was a risk factor for frequent ventricular premature beats(OR=1.203, P=0.013). Conclusion　When echocardiography suggests that LVFT is characterized by muscular type, oblique type or an angle greater than 30° with the ventricular septum, the patients are more likely to have frequent ventricular premature beats, which should be paid more attention to in clinical work and more reasonable treatment options should be selected.]]> 2020/2/18 17:19:01 GE Zhi-xiang, ZHU Yun, YANG Pan, et al. 33true ［Abstract］　Objective　To prepare a kit of time-resolved fluoroimmunoassa(TRFIA) for detection of transthyretin(TTR), and evaluate its effectiveness. Methods　The clone 4H2, the anti-TTR monoclonal antibody, was coated into 96-well microplate as the capture antibody, and the monoclonal antibody(clone 3E4) labeled with the Eu³⁺ as the detection antibody. A double-antibody sandwich TRFIA kit was prepared by sandwiching antigens to be tested between two detection antibodies. Then, the kit was used to detect the TTR levels of 42 children with Kawasaki disease(KD) and 13 healthy children, and the efficiency of the kit was evaluated using its indexes of dilution recovery, accuracy, precision and stability. Results　The TTR TRFIA kit and Western blot method were adopted to detect the same 42 KD samples and 13 healthy samples at the same time, and the coincidence rate between the two methods was 100%, with a high specificity. The minimum detectable concentration of the prepared TRFIA kit was 0.05 μg/ml with the dilution recovery rate being 88.00%～111.00%, and the intra-assay precision being 8.01%～9.17%, and the inter-assay precision being 8.88%～11.82%. The stability test showed that the kit could be stably stored at 4 ℃ for 6 months and 37 ℃ for 7 days. Conclusion　The TTR TRFIA kit prepared in this study has the advantages of high accuracy, convenience and quickness, which is suitable for TTR detection of a large amount of clinical KD serum samples, and provides a new method for differentiation of intravenous immunoglobulin G(IVIG)-sensitive and IVIG-non-responsive KD children.]]> 2020/2/18 17:19:01 CHEN Cui-cui, LIANG Huan-kun, LI Jie-xing, et al. 32true ［Abstract］　Objective　To observe the effects of butorphanol tartrate pretreatment on relieving rocuronium injection pain and haemodynamics during induction of anesthesia. Methods　A total of 90 selected patients who underwent endotracheal intubation under general anesthesia in our hospital from January 2019 to June 2019 were randomly divided into group B1(butorphanol 20 μg/kg), group B2(butorphanol 30 μg/kg) and group C(normal saline of the same quantity) by random number table method, with 30 cases in each group. The levels of mean arterial pressure(MAP), heart rate(HR) and percutaneous oxygen saturation(SpO₂) were compared among the three groups before giving butorphanol(T₀), immediately after injection of butorphanol(T₁), immediately before injection of rocuronium(T₂), immediately after injection of rocuronium(T₃) and 2 min after injection of rocuronium(T₄). The scores of injection pain and the incidence of adverse reactions were compared among the three groups using Ambesh score. Results　The MAPs of the three groups showed a trend of rising first and then declining. The amplitude of MAP in the group B1 was higher than that in the group B2 and lower than that in the group C, and the difference in the trend of change among the three groups was statistically significant(P<0.05). Group B1 and group B2 showed a slight increase after a slight decrease in HR, while group C showed a large increase at the time point of T₃. HR fluctuated less in the group B1 and B2 than in the group C, and the difference in the trend of change among the three groups was statistically significant(P<0.05). SpO₂ increased gradually in the three groups, and the group C showed a decrease at the time point of T₃, and the difference in the trend of change among the three groups was statistically significant(P<0.05). There was a significant difference in the incidence of injection pain among the three groups(P<0.05). The incidence of injection pain in group B1 and group B2 was significantly lower than that in group C(P<0.05). The incidence of injection pain in group B2 was significantly lower than that in group B1(P<0.05). There was no significant difference in the incidence of adverse reactions among the three groups(P>0.05). Conclusion　Butorphanol tartrate pretreatment is safe and effective in reducing the incidence of rocuronium injection pain and reducing hemodynamic fluctuation during induction of anesthesia, and the effect of 30 μg/kg is better than that of 20 μg/kg.]]> 2020/2/18 17:19:01 CHEN Cheng-zhe, REN Yi-feng, LI Hui-fang, et al. 31true ［Abstract］　Objective　To analyze human osteosarcoma cell microarray silenced by diacylglycerol kinase zeta(DGKZ) gene based on the bioinformatics method and explore its molecular mechanism. Methods　Human osteosarcoma cell microarrays were obtained from Gene Expression Omnibus(GEO) database. Differential expression genes(DEGs) in the DGKZ silencing treatment group and the control group were screened by R software. DEGs were submitted to DAVID database for gene function and pathway enrichment analysis. STRING database and Cytoscape software were used to construct protein-protein interaction(PPI) network and search for the hub genes in the network and predict hub gene transcription factors. Results　A total of 368 DEGs were screened out, including 105 up-regulated genes and 263 down-regulated genes. Gene Ontology(GO) enrichment analysis showed that the main biological processes of DEGs enrichment were negative regulation of osteoblast differentiation, angiogenesis, negative regulation of transcription from RNA polymerase Ⅱ promoter, positive regulation of fat cell differentiation, cell cycle and Wnt signaling pathway. KEGG pathway enrichment analysis suggested that the main pathways involved in DEGs were TGF-β signaling pathway, pathways in cancer, pancreatic cancer, viral carcinogenesis, MAPK signaling pathway and metabolic pathways. The hub genes of the top 10 were identified by STRING database and Cytoscape software as SIRT1, EP300, PTGS2, BMP2, HIF1A, RB1, HIST1H2BO, NT5E, H1F0, HIST1H2AC, and the highest standardized enrichment fraction of transcription factors predicted by iRegulon plug-in for hub genes was YY1. Conclusion　Transcription factor YY1 and the related target genes can play an important role in osteosarcoma under the condition of DGKZ silencing.]]> 2020/2/18 17:19:01 TONG Ye, XU Bin, LIU Xiao-wei 30true ［Abstract］　Objective　To explore the prognostic evaluation value of serum albumin to globulin ratio(AGR) in the prognosis of patients with acute exacerbation of chronic obstructive pulmonary disease(AECOPD). Methods　A retrospective study was conducted on the clinical data of 126 patients admitted to the hospital with AECOPD as the first diagnosis from January 2016 to May 2018. The optimal cut-off value of AGR for prognostic outcome was obtained by receiver operating characteristic(ROC) curve method, and the study subjects were divided into high AGR group(AGR≥1.295, n=76) and low AGR group(AGR<1.295, n=50). The clinical data, laboratory data and prognosis were compared between the two groups. Results　There were no significant differences in gender, age, smoking history, drinking history and sputum culture between the high AGR group and the low AGR group(P>0.05). The levels of C-reactive protein(CRP), interleukin-6(IL-6) and procalcitonin(PCT) in the high AGR group were significantly lower than those in the low AGR group(P<0.05). The number of co-infection, readmission due to acute exacerbation with 1 year of discharge from the hospital and death in the high AGR group was significantly less than those in the low AGR group(P<0.05). The hospitalization time of the high AGR group was shorter than that of the low AGR group(P<0.05). The levels of body mass index(BMI) and forced expiratory volume in 1 second(FEV1)% in the high AGR group were significantly higher than those in the low AGR group(P<0.05). The results of correlation analysis showed that the level of AGR was negatively correlated with CRP, IL-6, PCT and hospitalization time, and positively correlated with BMI and FEV1% in the AECOPD patients(P<0.05). Conclusion　Blood AGR can predict the prognosis of the patients with AECOPD and help to assess the lung function of the patients, which provides a good reference for guiding clinical treatment.]]> 2020/2/18 17:19:01 WANG Sai-nan, LIU Jian-bo, HAN Xiao-peng, et al. 29true ［Abstract］　Objective　To explore the efficacy and safety of extracorporeal shock wave(ESW) combined with Traditional Chinese Medicine Duhuo Jisheng decoction in treatment of knee osteoarthritis(KOA). Methods　Three hundred and sixty cases(445 knees) of KOA were divided into Chinese Medicine Duhuo Jisheng decoction treatment group(group A), simple ESW therapy group(group B), and ESW combined with Chinese Medicine Duhuo Jisheng decoction comprehensive treatment group(group C) according to the patients′ treatment intention, with 120 cases in each group. Visual Analogue Scale(VAS) scores and Lysholm function scores were measured in the three groups before and 2, 6, 12 and 24 weeks after treatment, and the concentrations of nitric oxide(NO) and superoxide dismutase(SOD) in the knee joint fluid were detected and compared among the three groups. Results　Compared with those before treatment, VAS scores decreased and Lysholm function scores increased 2, 6, 12, and 24 weeks after treatment in the three groups. There were significant differences in VAS scores and Lysholm function scores between group C and the other two groups(P<0.05). Compared with those before treatment, the concentrations of NO in the knee joint fluid were significantly decreased and the concentrations of SOD in the knee joint fluid were significantly increased in the three groups 2, 6, 12, and 24 weeks after treatment(P<0.05), and there was significant difference between group C and the other two groups(P<0.05). Conclusion　The comprehensive treatment of KOA by ESW combined with Chinese Medicine Duhuo Jisheng decoction is a non-invasive and feasible technology with many advantages. It has a definite clinical effect and is worth applying in clinics.]]> 2020/2/18 17:19:02 WANG Hong-run, LI Hong-yu, JIN Xian-yue, et al. 28true ［Abstract］　Objective　To analyse the disease composition and causes of nervous system diseases in children with nervous system diseases in Guangzhou City from 2006 to 2015, and provide the eugenics monitoring strategies and reproductive services for families with disabled children. Methods　Three thousand nine hundred and sixty-four cases of disabled children receiving medical expert testimony were collected from January 2006 to December 2015 in Guangzhou City, and their basic information, medical history and diagnosis were statistically analysed. Results　The number of disabled children with nervous system diseases or mental behavior disorders was 1 867, accounting for 39.05%～56.67% of the disabled children. The top five diseases were autism spectrum disorders(31.01%), mental retardation or delayed psycho neurogenesis(29.67%), cerebral palsy(13.12%), epilepsy(10.50%) and hereditary diseases(6.37%). Some cases might be related to perinatal factors, but there were still a lot of cases without definite risk factors. Conclusion　In recent years, the number of disabled children with autism spectrum disorders has increased significantly compared with that before 2010, which is related to the improvement of recognition and diagnosis ability of autism spectrum disorders, the change of genetics, various biological factors, social culture and environmental factors. Nearly half of the disabled are identified as nervous system diseases most of which have no risk factors identified. Health education and health care before and during pregnancy are the positive measures for clinical and health workers.]]> 2020/2/18 17:19:02 LIANG Qiu-fen, YANG Ye, QU Yan-xia, et al. 27true ［Abstract］　Objective　To investigate the clinical efficacy of with Shenshuai Mixture in treatment of nephron injury caused by high pressure perfusion in renal pelvis in kidney stone patients and its effect on renal function. Methods　Ninety-two patients with renal calculi admitted to our hospital from January 2017 to October 2018 were randomly divided into control group and observation group by random number table method, with 46 cases in each group. All the patients were treated with percutaneous nephrolithotripsy. The control group was treated with routine treatment of anti-inflammation after operation and the observation group was treated with the same treatment as the control group plus Shenshuai Mixture. The success rate of stone clearance and postoperative complications were compared between the two groups. The changes of the levels of serum creatinine(Scr), blood urea nitrogen(BUN), urine neutrophil gelatinase-associated lipocalin(uNGAL) and beta-2-microglobulin(β₂-MG) were compared before and 2 months after operation. Results　The success rate of stone clearance was 93.49% in the control group and 95.65% in the observation group, and there was no significant difference in the incidence of complications after operation between the control group(8.70%) and the observation group(6.51%)(P>0.05). The levels of Scr, BUN, uNGAL and β₂-MG in the observation group were significantly lower than those in the control group 2 months after operation(P<0.05). Conclusion　Compared with the control group, Shenshuai Mixture can reduce the Scr and BUN indexes of the patients with nephron injury caused by high pressure perfusion in renal pelvis after nephrolithiasis operation, improve the renal function, and the clinical effect is satisfactory.]]> 2020/2/18 17:19:02 SHANG Cui-qing, ZHU Jin-yan, LIU Yi-peng, et al. 26true ［Abstract］　Objective　To investigate the therapeutic effect of syndesmotic screw fixation on treatment of Maisonneuve fracture. Methods　The clinical data of 21 patients with Maisonneuve fracture admitted to the Department of Osteoarthropathy, Shenzhen People′s Hospital from October 2014 to November 2018 were retrospectively analyzed. Before operation, X-ray films of the affected ankle and the full-length tibiofibula, computed tomography(CT) three dimensional reconstruction of the ankle joint and magnetic resonance imaging of the ankle joint were completely examined. There were 14 cases of deltoid ligament injury, 7 cases of medial malleolus fracture, 15 cases of posterior inferior tibiofibular ligament injure and 6 cases of posterior malleolus fractures. Two cortical bone screws were used to fix the lower tibiofibular joint. The medial malleolus fractures and posterior malleolus fractures involving more than 25% of the articular surface were treated with 2 screws to fix the fracture block. The ankle joint was fixed with short leg cast for 4 weeks after operation. After 12 weeks, the lower tibiofibular screw was removed. Postoperative outpatient follow-up was conducted for 12 to 29 months, with an average of 18.31 months. X-ray films were regularly reviewed. Visual Analogue Scale(VAS) score and American Orthopaedic Foot & Ankle Society(AOFAS) ankle-hindfoot score were performed before and after surgery to evaluate the treatment effects. Results　The anatomic reposition of ankle joints was achieved after operation. The joint matching was normal, and no complications such as incision infection and fracture of lower tibiofibular screw occurred. Regular reexamination of X-ray showed fracture healing. The ankle joint matching relationship was normal, and no reduction loss occurred. The patients′ VAS scores showed a downward trend after operation, and the AOFAS ankle-hindfoot scores showed an upward trend. Pain relief and ankle movement function improved significantly(P<0.05). Conclusion　The treatment of Maisonneuve fracture with tibiofibular screw fixation can restore the normal anatomic relationship of the ankle joints with satisfactory results.]]> 2020/12/31 15:05:24 REN Jin-ke, XIAO Ting-hui, JIA Zhao-feng, et al. 25true ［Abstract］　Objective　To compare the clinical value between indirect immunofluorescence method and particle agglutination in diagnosis of Mycoplasma pneumoniae(MP) infection in children. Methods　Five hundred and thirty-nine children with MP infection who were admitted to Department of Pediatrics, the Affiliated Hospital of Jiangsu University from January 2019 to December 2019 were selected, and their serum samples were detected by indirect immunofluorescence method and particle agglutination at the same time, and the results were compared between the two methods. Results　The positive rate of MP-IgM detected by indirect immunofluorescence method was 70.69%(381/539), and the positive rate of MP-IgM detected by particle agglutination was 73.10%(394/539), and there was no statistically significant difference in the detection result between the two methods(P=0.409). The positive rates of MP-IgM detected by indirect immunofluorescence method in children aged 0 to 2 years, 3 to 5 years, and ≥6 years were 70.00%, 78.51% and 60.25%, respectively. The positive rates of MP-IgM detected by particle agglutination were 70.67%, 76.32% and 70.81%, respectively. There were no significant differences in the detection results between the two methods in 0-2 years old group and 3-5 years old group(P>0.05). There were significant differences in the detection results between the two methods in the ≥6 years old group(P<0.05). For the children with disease courses of ≤3 days, 4-7 days, 8-14 days and ≥15 days, the positive rates of MP-IgM detected by indirect immunofluorescence method were 61.90%, 68.10%, 82.35% and 84.09%, respectively, and the positive rates of MP-IgM detected by particle agglutination were 65.48%, 70.86%, 83.53% and 84.09%, respectively; there were no significant differences in the detection results between the two methods(P>0.05), and the positive rates of the detections showed an increasing trend as the course of the disease prolonged. Conclusion　Both indirect immunofluorescence method and particle agglutination can be used to detect MP infection in children. The course of disease from 8 to 14 days is a better detection time window, which can avoid missed diagnosis to a greater extent and start treatment as soon as possible.]]> 2020/12/31 15:05:24 LU Jun-ying, LU Hong-yan, CHANG Ming, et al. 24true ［Abstract］　Objective　To establish a method for the determination of linagliptin in rabbit plasma by ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS). Methods　After the rabbit plasma samples were precipitated with acetonitrile, diazepam was used as the internal standard for the determination of linagliptin. Acquity UPLC BEH C18 column was used as the separation column. The gradient elution of linagliptin was performed using a mobile phase of acetonitrile-0.1% formic acid. The total running time was 3.0 min, and the eluting time of linagliptin was 1.09 min. Acquity Xevo TQD triple quadrupole mass spectrometer was used for detection. The concentration of the drug in the samples was determined by multiple reaction monitoring(MRM). The specificity, linearity, precision, accuracy, recovery and stability of the method were investigated. Results　The chromatogram showed that the retention time of linagliptin in plasma was 1.09 min, without the interference of endogenous peaks. The typical equation of the calibration curve was (y=0.1646x+0.0303, r=0.9914), with a good linear relationship. The within-day and between-day precisions and accuracies were less than 15%. The drug recovery rate was in the range of 76.0%～82.1%. The deviation of the drug concentrations under different storage conditions was within ±15% of the mean value, which was relatively stable. Conclusion　The method is simple and can be used for rapid determination of linagliptin in rabbit plasma, pharmacokinetic research and drug interaction study.]]> 2020/12/31 15:05:24 ZHENG Xiao-zhen, LIU Jing, LI Ming-ming, et al. 23true ［Abstract］　Objective　To analyze the incidence of birth defects in Guangxi from 2011 to 2015. Methods　Four million three hundred and fifteen thousand one hundred and thirty-four cases of fetuses and newborns(7 days after birth) recorded in the Guangxi Women′s and Children′s Health Service Information Management System from January 1, 2011 to December 31, 2015 were selected, and their data of birth(including defect type, gender, birth time, regional source, etc.) were analyzed. Results　Among the 4 315 134 perinatal infants who were monitored, 57 851 cases were diagnosed as defective infants(134.07/10⁴). Among them, the incidence of birth defects in each year from 2011 to 2015 was 137.48/10⁴, 120.80/10⁴, 109.57/10⁴, 131.32/10⁴ and 174.03/10⁴, respectively. Most of the newborns with birth defects were male(33327/57851,57.61%), the Han nationality(37930/57851, 65.56%) and the Zhuang nationality(17284/57851, 29.88%). The top five cities in Guangxi having the newborns with birth defects from 2011 to 2015 were Nanning(13776/57851, 23.81%), Liuzhou(6366/57851, 11.00%), Yulin(5711/57851, 9.87%), Guilin(4644/57851, 8.03%) and Wuzhou(4254/57851, 7.35%). The defect types mainly included polydactyly(toes), congenital heart disease, fetal edema syndrome, cleft lip, cleft lip with cleft palate, talipes equinovalgus and other malformations of the outer ear, among which polydactyly(toes) and congenital heart disease accounted for the largest proportion. Conclusion　The incidence of birth defects in Guangxi is on the rise, especially polydactyly(toes) and congenital heart disease. It is necessary to do a good job in the propaganda of the related knowledge of pregnancy and childbirth, increase the rate of prenatal diagnosis, and improve the quality of the newborn populations.]]> 2020/12/31 15:05:24 WEI Yu-chen, WEI Ping, HUANG Xu-hua, et al. 22true ［Abstract］　Objective　To explore the changes of circulating tumor cells(CTCs) in peripheral blood of the patients with rectal cancer during the perioperative period and its influence on the patients′prognosis. Methods　Thirty patients with rectal cancer admitted to the Department of Gastrointestinal Surgery, the People′s Hospital of Guangxi Zhuang Autonomous Region were selected from September 2015 to December 2016, and were divided into laparoscopic surgery group and laparotomy group by random number table method, with 15 cases in each group. The classification and detection of CTCs in peripheral blood of the patients with rectal cancer were performed using CanpatrolTM 2nd generation technology before and after surgery. The patients were followed up for 22-33 months to analyze the changes of CTCs levels before and after surgery in the two groups. The correlation between each type of CTCs before surgery and disease characteristics, and the impacts of CTCs on the prognosis of the patients before and after surgery were analyzed. Results　The level of total CTCs in the laparoscopic surgery group after surgery was significantly lower than that before surgery(P<0.05). The level of mixed type CTCs in the laparoscopic surgery group was significantly higher than that in the laparotomy group before surgery(P<0.05), but there was no statistically significant difference between the two groups after surgery(P>0.05). There were no statistically significant differences in the levels of interstitial type CTCs and epithelial type CTCs between the two groups before and after surgery(P>0.05), and there were no significant differences in the same group before and after surgery(P>0.05). The proportion of the patients with tumor diameter ≤3 cm in the interstitial type negative group was significantly higher than that in the interstitial type positive group(P<0.05). The preoperative total CTCs, mixed type CTCs, and epithelial type CTCs were not significantly correlated with the patients′ age, gender, T staging, N staging and tumor diameter(P>0.05). Three cases of postoperative CTCs negative patients did not die during the follow-up period, but among 27 cases of postoperative CTCs positive patients, 4 cases died during the follow-up period. The survival of the postoperative CTCs negative patients was significantly better than that of the positive patients(log-rank test: χ²=6.553, P=0.031). Two patients with negative CTCs before surgery did not die during the follow-up period, but 4 of 28 patients with positive CTCs before surgery died during the follow-up period. The survival of the preoperative CTCs negative patients was significantly better than that of the preoperative CTCs positive patients(log-rank test: χ²=5.245, P=0.027). Conclusion　Peripheral blood CTCs can be used to evaluate the prognosis of the patients with rectal cancer and guide the clinical treatment.]]> 2020/12/31 15:05:24 HAN Ya-min, YIN Wu, WEI Bin, et al. 21true ［Abstract］　Objective　To explore the changes of serum matrix metalloproteinase 9(MMP-9), tissue inhibitor of matrix metalloproteinase 1(TIMP-1) and interleukin-17(IL-17) levels in children with bronchiolitis and their clinical significances. Methods　Fifty-six children with bronchiolitis admitted to our hospital from October 2018 to March 2019 were selected and divided into common group(32 cases, including children with mild symptom) and severe group(24 cases, including children with moderate and severe symptoms), and 30 healthy children receiving physical examination in the outpatient department in the same period were taken as the control group. The serum levels of MMP-9, TIMP-1 and IL-17 were detected by enzyme-linked immunosorbent assay(ELISA) in the three groups. The correlation between MMP-9, TIMP-1, IL-17 and the severity of bronchiolitis, and their diagnostic efficacy for bronchiolitis were investigated. Results　The serum levels of MMP-9, TIMP-1 and IL-17 in the common group and the severe group were higher than those in the control group, and the differences were statistically significant(P<0.05). The serum levels of MMP-9, TIMP-1 and IL-17 in the severe group were higher than those in the common group, and the differences were statistically significant(P<0.05). Spearman correlation analysis showed that MMP-9(r_s=0.799), TIMP-1(r_s=0.731) and IL-17(r_s=0.756) were positively correlated with the severity of the disease(P<0.05). The results of receiver operating characteristic(ROC) curve analysis showed that the serum MMP-9, TIMP-1 and IL-17 had reference significance for the diagnosis of bronchiolitis(P<0.05), among which the area under curve(AUC) of MMP-9 was the largest and the diagnostic efficiency was the best. Conclusion　Dynamic observation of serum MMP-9, TIMP-1 and IL-17 levels in children can evaluate the severity of the disease, and it has positive significance for the early detection and early treatment of bronchiolitis.]]> 2020/12/31 15:05:24 ZHAO Shao-cong, LI Wan-wan, LIU Zhen, et al. 20true ［Abstract］　Objective　To explore the abnormalities of brain structure in patients with chronic schizophrenia. Methods　Fifty patients with chronic schizophrenia admitted to Zigong Mental Health Center from January 2017 to August 2019 were selected as the research group, and other 50 healthy volunteers were selected as the control group. Both groups underwent brain magnetic resonance imaging(MRI) scans. The width of the third ventricle, the width of the head of the caudate nucleus, the distance between the anterior angles of the ventricle, the distance between the triangular regions, the width of the bilateral lateral fissure, the height of the hippocampus, the thickness of the corpus callosum and other indicators were measured by linear measurement method, and were compared between the two groups. Results　The width of the third ventricle, the width of the head of the left caudate nucleus, the width of the head of the right caudate nucleus, the width of the left lateral fissure, and the width of the right lateral fissure in the research group were significantly larger than those in the control group(P<0.05). There were no significant differences between the two groups in the distance between the anterior angles of the ventricle, the distance between the triangular regions, the height of the left hippocampus, the height of the right hippocampus, and the thickness of the corpus callosum(P>0.05). Conclusion　There are brain structural abnormalities in the patients with chronic schizophrenia. MRI linear measurement has certain significance for the diagnosis and differential diagnosis of chronic schizophrenia.]]> 2020/12/31 15:05:24 ZHAN Kong-cai, ZOU Yan, ZHOU Wei-qiang, et al. 19true ［Abstract］　Objective　To investigate the infection of human papilloma virus(HPV) in patients with cervical intraepithelial neoplasia in Guigang area of Guangxi, and to provide theoretical basis for the health and disease control departments in this area to formulate HPV vaccination and cervical cancer relevant prevention and control strategies. Methods　From January 2012 to September 2017, 1 985 patients with cervical intraepithelial neoplasia confirmed by pathological biopsy in Guigang City People′s Hospital and Guigang Traditional Chinese Medicine Hospital were selected. The age of the patients ranged from 19 to 81 years old. All the patients were registered in Guigang City, Guangxi. All the patients did not receive radiotherapy and chemotherapy before the confirmed diagnosis of pathological biopsy, and the results of their HPV genotyping and pathological examination were collected. According to the results of the pathological examination, the research subjects were divided into low-grade intraepithelial neoplasia group(1 012 cases) and high-grade intraepithelial neoplasia group(973 cases). HPV infection rate and the infection of different HPV subtypes were analyzed in the two groups. The correlation between HPV infection rate and the patients′ age was analyzed. Results　A total of 1 636 cases were infected with HPV in this study, and the infection rate was 82.4%. The HPV infection rate was 87.8% in the high-grade intraepithelial neoplasia group, and 77.3% in the low-grade intraepithelial neoplasia group, with a significant difference between the two groups(P<0.05). The single infection rate and the multiple infection rate in the high-grade intraepithelial neoplasia group were 56.1% and 31.7%, respectively, and those in the low-grade intraepithelial neoplasia group were 50.2% and 27.1%, respectively, with significant differences between the two groups(P<0.05). The infection rates of high-risk and low-risk subtypes in the high-grade intraepithelial neoplasia group were 87.4% and 6.5%, respectively, and those in the low-grade intraepithelial neoplasia group were 73.1% and 15.9%, respectively, with significant differences between the two groups(P<0.05). The HPV genotype test results showed that the main genotypes(top 5 positions) detected in the high-grade intraepithelial neoplasia group were HPV-16, HPV-58, HPV-52, HPV-33, and HPV-18, and those detected in the low-grade intraepithelial group(top 5 positions) were HPV-52, HPV-58, HPV-16, HPV-51 and HPV-18, all of which were high-risk subtypes. Spearman correlation analysis showed that the patients′ age was negatively correlated with the HPV infection rate(r_s=-0.900, P=0.037). Conclusion　HPV infection is an important pathogenic factor for cervical intraepithelial neoplasia in women in Guigang area of Guangxi. HPV-16, HPV-58, HPV-52, HPV-33 and HPV-18 subtypes are the main epidemic subtypes in this area, and young and middle-aged women are the mainly infected people. It is suggested that the local women choose the nine-valent HPV vaccine for vaccination.]]> 2020/12/31 15:05:24 YANG Da-ping, LIU Yong-qing, LIANG Jin-yan, et al. 18true ［Abstract］　Objective　To analyze the clinical characteristics of patients with severe/critical 2019 corona virus disease(COVID-19) in Guangxi area, and provide the reference for clinical diagnosis and treatment of severe/critical COVID-19 patients. Methods　The medical history, laboratory examination and imaging data of 9 patients with severe/critical COVID-19 admitted to Yongwu Hospital of the People′s Hospital of Guangxi Zhuang Autonomous Region from Febuary 16, 2020 to March 16, 2020 were collected and the clinical diagnosis and treatment experience was summarized. Results　Nine patients with severe/critical COVID-19 were 41～85 years old, including 5 males and 4 females. Among the 9 cases, 2 cases were severe and 7 cases were critical.Compared with the severe COVID-19 patients, the critical COVID-19 patients had relatively higher neutrophil ratio, D-dimer, procalcitonin and lactate dehydrogenase on admission, and relatively lower lymphocyte count and serum albumin. Two cases of severe COVID-19 needed nasal catheter/mask oxygen inhalation; 2 cases of critical COVID-19 needed high flow oxygen inhalation and 5 cases of critical COVID-19 needed invasive mechanical ventilation. Two cases of critical COVID-19 needed extracorporeal membrane oxygenation(ECMO) support therapy. Six critical patients were complicated with acute respiratory distress syndrome(ARDS), pulmonary bacterial and fungal infection, and multiple organ dysfunction syndrome. The prognosis was evaluated 28 days after admission, and 7 cases were cured and discharged. Up to March 16, 2 cases of critical COVID-19 were cured and transferred to the Intensive Care Unit(ICU) of Non-negative Pressure Ward for treatment of their basic diseases. Conclusion　Comprehensive and individualized treatments can effectively improve the prognosis of severe/critical COVID-19 patients.]]> 2020/12/31 15:05:24 ZHANG Ji-wang, XIANG Shu-lin, LU Zheng, et al. 17true ［Abstract］　Objective　To investigate the polymorphisms of Toll-like receptor 2(TLR2) gene rs2289318G/C and rs3804100T/C in Guangxi Zhuang population, and to compare their distribution differences with different regions and races. Methods　Single base extension technology and DNA sequencing were used to detect the genotypes of rs2289318G/C and rs3804100T/C of TLR2 gene in 210 healthy persons of Guangxi Zhuang nationality receiving health examination. The genetic polymorphisms were analyzed and the results were compared with the genotypes of African Ancestry in Southwest US(ASW), the ancestry of African Caribbean in Barbados(ACB), Yoruba in Ibadan, Nigeria(YRI), American(AMR), Japanese in Tokyo, Japan(JPT), European(EUR), South Asian(SAS) and Han Chinese in Beijing, China(CHB) published by Thousand Human Genome Project Database. Results　There were GG, CG and CC genotypes at the rs2289318G/C locus of TLR2 gene in Guangxi Zhuang population, the frequencies of which were 58.57%, 37.62%, and 3.81%, respectively; the frequencies of G and C alleles were 77.38% and 22.62%, respectively. There were TT, CT and CC genotypes in the locus of rs3804100T/C, and their frequencies were 57.62%, 38.10% and 4.29%, respectively; the frequencies of T and C alleles were 76.67% and 23.33%, respectively. In the Guangxi Zhuang population, there were no significant differences in the genotype and allele frequencies of rs2289318G/C and rs3804100T/C loci of TLR2 gene between different genders(P>0. 05). There was a statistically significant difference between the rs2289318G/C allele in the Guangxi Zhuang population and that in SAS(P<0.05), but there were no statistically significant differences between the genotypes at the rs2289318G/C locus of TLR2 gene in the Guangxi Zhuang population and those in SAS(P>0.05). There were no statistically significant differences in the rs2289318G/C allele and its allele frequencies between the Guangxi Zhuang population and ACB, ASW, YRI, AMR, JPT, EUR and CHB(P>0.05). There were statistically significant differences in the genotypes at the rs3804100T/C locus of TLR2 gene and its allele frequencies between the Guangxi Zhuang population and ACB, ASW, YRI, AMR, EUR, SAS and CHB(P<0.05). However, there were no statistically significant differences in the genotypes at the rs3804100T/C locus of TLR2 gene and its allele frequencies between the Guangxi Zhuang population and JPT(P>0.05). Conclusion　The polymorphisms of TLR2 gene rs2289318G/C and rs3804100T/C in Guangxi Zhuang population are different from those in different ethnic groups.]]> 2020/12/5 15:52:01 LU Jiang-yu, JIANG Yu-jie, HUANG Xia, et al. 16true ［Abstract］　Objective　To analyze the follow-up results of patients with human immunodeficiency virus 1(HIV-1) antibody indeterminate, and to provide the basis for rapid diagnosis and treatment. Methods　The clinical data of 37 cases of indeterminate HIV-1 antibody treated in Longtan Hospital of Guangxi Zhuang Autonomous Region from January 2019 to June 2020 were summarized, and their diagnosis and treatment were analyzed, and their clinical characteristics were summarized. Results　The results of the first confirmed test of 37 HIV-1 antibody screening positive patients were all judged to be uncertain. After follow-up and nucleic acid test, 21 cases were confirmed to be human immunodeficiency virus(HIV)/acquired immune deficiency syndrome(AIDS) patients, and 15 cases were confirmed to be diseases other than HIV-1, and one case did not come to the hospital for further consultation. Among the 21 HIV/AIDS patients, 11 cases were complicated with opportunistic infection and 10 cases were asymptomatic. After follow-up, the time from the initial screening positive for HIV-1 antibody to the confirmation of HIV-1 positive by Western blot(WB) in the 21 HIV/AIDS patients was 70(40.0, 152.5) days, and the time from HIV-1 antibody screening positive to nucleic acid positive was 10(2, 15)days, and the difference was statistically significant between both of them(Z=4.318, P=0.000). The CD4⁺ T lymphocyte count and S/CO value of initial screening test in the 21 HIV/AIDS patients were(191.10±154.55)/mm³ and 71.93(27.46, 150.87), respectively, and those in the patients excluded from HIV-1 infection were (422.07±219.68)/mm³ and 1.53(1.30, 1.98), respectively. The differences were statistically significant between both of them(P<0.05). The 13 HIV/AIDS patients received antiretroviral therapy(ART) in the hospital. After 6 months of the treatment, the CD4⁺ T lymphocyte count of the patients increased to (403.23±237.05)/mm³, and the virus inhibition rate was 92.31%. Conclusion　Antibody testing is still the main method for the diagnosis of HIV-1 infection, and the uncertainty of antibody results increases the difficulty in the diagnosis of HIV/AIDS. Timely follow-up and confirmatory test or nucleic acid test should be conducted for the uncertainty of HIV-1 antibody results, which can provide evidence for early diagnosis and treatment of HIV-infected patients.]]> 2020/12/5 15:52:01 MENG Zhi-hao, LIAO Guang-fu, LAN Ke, et al. 15true ［Abstract］　Objective　To analyze the clinical efficacy of Ilizarov transverse tibial bone transport technique combined with interventional methods in treatment of severe lower limb arterial vascular lesions of diabetic foot. Methods　Twenty-one patients with diabetic foot ulcers admitted to the Second Affiliated Hospital of Luohe Medical College from September 2015 to January 2019 were selected and were treated with Ilizarov transverse tibial bone transport technique combined with interventional methods. The wound healing time, ankle-brachial index, lower limb blood vessel diameter and blood flow velocity before and 6 months after the operation were recorded to evaluate the clinical efficacy. Results　Two weeks after the operation, the ulcer surfaces of the patients began to shrink, and 4 weeks after the operation, the skin graft or flap transplantation conditions reached. No special treatment was performed on all the wounds. The ulcer surfaces of all the patients healed, and the wound healing time was (57.76±12.3)days. Two months after the operation, the results of ultrasound reexamination showed that the peripheral neovascular nets of the affected limbs were abundant, and the function of the affected limbs met the needs of daily life. Six weeks after the operation, Doppler blood flow detector found an increase in the ankle-brachial index of the affected feet; vascular ultrasound found increased blood flow in the anterior and posterior tibial arteries of the affected feet, and the inner diameter of the blood vessels increased; there were statistically significant differences between the postoperative results and the preoperative results(P<0.05). One case died of severe complications. Conclusion　For diabetic foot patients with severe stenosis or even occlusion of the large arteries above the knee joint of the lower extremities, interventional methods can be used to recanaliza the large blood vessels, and then Ilizarov transverse tibial bone transport technique is used to improve microcirculation, speed up wound repair, and reduce the incidence of small and large amputations.]]> 2020/12/5 15:52:01 LI Zhen, LING Jian-sheng, WANG Peng-ru, et al. 14true ［Abstract］　Objective　To investigate the risk of congenital heart disease(CHD) in non-assisted reproductive technology pregnancy with monochorionic diamniotic(MCDA) twins. Methods　A retrospective cohort study was conducted to include the neonates admitted to the Neonatal Intensive Care Unit of Shenzhen Maternity and Child Healthcare Hospital from January 2014 to December 2019, including 6107 cases of single births, 834 cases of dichorionic diamniotic(DCDA) twins, 590 cases of MCDA twins and 84 cases of MCDA twins complicated with twin-to-twin transfusion syndrome(TTTS). The incidence and relative risk of CHD were compared among the four groups, as well as the disease types of CHD patients in the four groups. Results　The incidence rates of CHD were 3.01%, 2.88%, 5.08%, and 21.43% in the singleton group, DCDA twins group, MCDA twins group and MCDA twins complicated with TTTS group, respectively. The difference was statistically significant among the four groups(χ²=94.676, P=0.000). When the singleton group was taken as reference, the risk of CHD in the MCDA twins group and the MCDA twins complicated with TTTS group was 1.69 and 7.11 times higher than that of the singleton group; when the DCDA twins group was taken as reference, the risk of CHD in the MCDA twins group and the MCDA twins complicated with TTTS group was 1.77 and 7.45 times higher than that in the DCDA twins group. The type of CHD in the singleton group was mainly ventricular septal defect(39.67%) and the other types of CHD(35.33%); the main type of CHD in the DCDA twins group was ventricular septal defect(66.67%); the MCDA twins group and the MCDA twins complicated with TTTS group was the other types of CHD(46.67%, 44.44%). Conclusion　MCDA twins, especially MCDA twins complicated with TTTS, have a higher risk of CHD. Clinicians should pay attention to the occurrence of CHD in the diagnosis and treatment of such children patients.]]> 2020/12/5 15:52:01 CHEN Chun, SUN Hong-yan, ZHANG Su-er, et al. 13true ［Abstract］　Objective　To observe the curative effect of tightening medial patellar ligament with suture anchor and lateral patellar retinaculum release on treatment of patellofemoral osteoarthritis. Methods　Thirty-seven patients with patellofemoral osteoarthritis admitted to Liuzhou Municipal Liutie Central Hospital Affiliated to Guangxi Medical University were selected from January 2015 to February 2019 and received tightening medial patellar ligament with suture anchor and lateral patellar retinaculum release. The Lysholm score, knee injury and osteoarthritis outcome score(KOOS), lateral patellofemoral angle(LPFA) and patellofemoral congruence angle(PFCA) of the patients were compared between the results before surgery and the results 1 year after surgery. Results　All the patients had no complications during the follow-up period. One year after surgery, the patients′ Lysholm score, KOOS and LPFA level were significantly higher than those before surgery(P<0.05), and PFCA was significantly lower than that before surgery(P<0.05). Conclusion　Tightening medial patellar ligament with suture anchor and lateral patellar retinaculum release is a simply operational and satisfactory combined method of treating patellofemoral osteoarthritis, and is worthy of clinical application.]]> 2020/12/5 15:52:01 ZHANG Zhong-xing, YANG Xiao-long 12true ［Abstract］　Objective　To observe the clinical effects of loose seton placement and multi-incision drainage on treatment of complex perianal abscess. Methods　Sixty-four patients with perianal abscess admitted to the People′s Hospital of Macheng from January 2018 to September 2019 were included in this study, and were randomly divided into observation group and control group, with 32 cases in each group. The observation group received loose seton placement and the control group was treated with conventional multi-incision drainage. The operation-related situation, pain score, anal function score, quality of life index score and the incidence of complications were compared between the two groups. Results　There were no significant differences in the operation time, hospitalization period and hospitalization expenses between the two groups(P>0.05). The amount of intraoperative bleeding, total number of dressing changes and the number of >2 cm scar in the observation group were significantly better than those in the control group(P<0.05). The severity degrees of pain in the observation group were lower than those in the control group 1 and 7 days after operation, and the differences were statistically significant(P<0.05). The Wexner anal incontinence scores of the observation group were better than those of the control group 1 week and 8 weeks after operation, and the differences were statistically significant(P<0.05). The gastrointestinal quality of life index scores of the observation group were significantly better than those of the control group 2 weeks and 8 weeks after operation(P<0.05). There was no significant difference in the overall incidence of postoperative complications between the two groups(χ²=0.674, P>0.05). Conclusion　Compared with that of traditional multi-incision drainage, the short-term curative effect of loose seton placement has certain advantages, which provides a more simple and minimally invasive treatment for perianal abscess.]]> 2020/12/5 15:52:01 TAO Qi, CHEN Li-min, ZHENG Wu, et al. 11true ［Abstract］　Objective　To construct a three-dimensional(3D) nonlinear finite element model of maxillary correction system with asymmetric rocking chair arch wire, and to lay the foundation for further investigating the biomechanical mechanism of asymmetric rocking chair arch wire on the dentition. Methods　Computed tomography(CT) scan data of maxilla and upper dentition, and the data of real items including bracket, arch wire and so on were used to construct a 3D nonlinear finite element model of the whole maxilla including the maxilla, dentition, brackets and asymmetric rocking chair arch wire through running special software such as 3D finite element analysis. Results　The 3D nonlinear finite element model of maxillary correction system with asymmetric rocking chair arch wire was constructed. The model had a total of 160 514 units and 242 878 nodes. Conclusion　The constructed finite element model has a high degree of simulation and good geometric similarity, which provides a good research method for further study of the mechanical mechanisms of asymmetric rocking chair arch wire acting on the dentition when the asymmetric rocking chair arch wire is used to correct the occlusal plane deflection.]]> 2020/12/5 15:52:01 ZENG Qing-yan, FANG Zhi-xin, ZHOU Yan, et al. 10true ［Abstract］　Objective　To understand the cognition situation of medical staff on patient safety culture and its influencing factors, so as to provide scientific basis for further improvement and promotion of hospital safety culture. Methods　By cluster sampling, 500 medical personnel from clinical, medical and technical departments and administrative logistics departments of the class A tertiary comprehensive hospital were selected. The anonymous survey was conducted with the Hospital Survey on Patient Safety Culture(HSOPSC). Results　Five hundred questionnaires were issued and 488 valid questionnaires were collected. There were statistically significant differences in the scores of HSOPSC among the medical staff with different genders, departments, professional titles, working years in the hospital and working years in the department(P<0.05). Multiple linear regression analysis results showed that female was a contributing foctor to high HSOPSC scores, and longer working years in the departments promoted the occurrence of low HSOPSC scores. Conclusion　The cognition of patient safety culture in the medical staff of the hospital is at the upper middle level. The disadvantages should be paid attention to and the defects should be improved, and the medical safety training should be strengthened. The unimpeded and unpunished error reporting mechanism should be improved, and the long-term evaluation mechanism of hospital safety culture should be established and improved to promote the continuous improvement of the medical quality and safety level.]]> 2020/12/5 15:52:01 CHEN Wu-chao, LIN Ying-zhong, ZHANG Guo, et al. 9true ［Abstract］　Objective　To investigate the expressions and clinical significances of interleukin-34(IL-34), receptor activator of nuclear factor κB ligand(RANKL), and osteoprotegerin(OPG) in the serum and prosthetic boundary membrane tissues of the patients with aseptic loosening after artificial hip replacement. Methods　From October 2018 to June 2020, 10 patients in our hospital who needed revision of hip prosthesis due to aseptic loosening of hip prosthesis after hip replacement surgery were selected as research group, and 10 patients who underwent initial total hip replacement due to femoral head necrosis or femoral neck fracture in our hospital during the same period were included as control group. Before operation, 5 milliliters of venous blood were taken from both groups; during operation, the boundary membrane tissues around the acetabular cup were taken from the research group, and the fascia and joint capsule tissues around the acetabulum were taken from the control group for further examination. The expressions of IL-34, RANKL and OPG in the tissue samples and serum were compared between the two groups. Results　The results of enzyme-linked immunosorbent assay(ELISA) showed that the serum IL-34, RANKL and OPG levels in the research group were significantly higher than those in the control group(P<0.05). The real-time quantitative polymerase chain reaction(RT-PCR) test results showed that the relative expression levels of IL-34, RANKL and OPG mRNA in the tissue samples of the research group were significantly higher than those of the control group, and the RANKL/OPG value was (1.21±0.36) in the research group, and (0.59±0.24) in the control group, with a significant difference between the two groups(P<0.05). Conclusion　IL-34 is both highly expressed in the serum and prosthetic boundary membrane tissues of the patients with aseptic loosening after artificial hip replacement, which may be involved in the pathogenesis of aseptic loosening together with RANKL and OPG.]]> 2020/11/3 8:38:30 LIU Zi-ge, GUO Feng-ying, ZHANG Chen, et al. 8true ［Abstract］　Objective　To analyze the ultrasound and laboratory examination results of patients with differentiated thyroid cancer(DTC), and to explore the factors affecting cervical lymph node metastasis of DTC. Methods　The data of 133 patients who underwent surgical treatment in our hospital from January 2019 to December 2019 and were diagnosed with DTC by postoperative pathology were selected and the patients were divided into metastasis group(78 cases) and non-metastasis group(55 cases) according to whether there was cervical lymph node metastasis confirmed by pathology results. The general clinical data, laboratory examination data and ultrasonic examination data in the two groups were collected and were compared between the two groups. The factors affecting DTC cervical lymph node metastasis of DTC were analyzed by multivariate Logistic regression. Results　Compared with the non-metastasis group, the metastasis group had larger proportion of male, younger patients, larger proportion of the patients with intranodular calcification and larger diameter of the nodules, and the differences were statistically significant(P<0.05). The levels of serum thyroid stimulating hormone(TSH), the positive rates of anti-thyroglobulin antibodies(TGAb) and anti-thyroid peroxidase antibody(TPOAb) in the metastasis group were higher than those in the non-metastasis group, and the differences were statistically significant(P<0.05). The results of multivariate Logistic regression analysis showed that the occurrence of intranodular calcification, positive TGAb, and larger diameter of the nodules were the risk factors for promoting the occurrence of cervical lymph node metastasis of DTC(P<0.05); while higher age and female were the protective factors for cervical lymph node metastasis of DTC(P<0.05). Conclusion　Male, low age, large diameter of the nodules, intranodular calcification, and positive TGAb are the risk factors for the occurrence of cervical lymph node metastasis in the patients with DTC. The necessary preoperative ultrasonic and serological examinations are of great significance to guide DTC operation and prognosis assessment.]]> 2020/11/3 8:38:30 XUE Ming-miao, SHANG Meng-yuan, WANG Zhi-hua, et al. 7true ［Abstract］　Objective　To compare the clinical efficacy of high-flow nasal cannula oxygen therapy(HFNC) and non-invasive positive pressure ventilation(NIPPV) in treatment of acute exacerbation of chronic obstructive pulmonary disease(AECOPD) complicated with type Ⅱ respiratory failure, and to analyze the influencing factors of failure of HFNC in treatment of AECOPD complicated with type Ⅱ respiratory failure. Methods　One hundred and twenty patients with AECOPD complicated with type Ⅱ respiratory failure admitted to the Department of Critical Care Medicine of Wuwei People′s Hospital from January 2018 to December 2019 were selected and divided into HFNC group and NIPPV group by random number table method, with 60 cases in each group. The changes in respiratory rate(RR), procalcitonin(PCT), arterial blood gas analysis related indexes, Acute Physiology and Chronic Health Evaluation Ⅱ(APACHE Ⅱ) scores and Sequential Organ Failure Assessment(SOFA) scores were compared between the two groups before and after treatment. The 72-hour fluid balance volume, non-invasive mechanical ventilation time, invasive mechanical ventilation rate and invasive mechanical ventilation time were compared between the two groups after treatment. Multivariate Logistic regression analysis was used to analyze the factors influencing the treatment failure of HFNC. Results　Compared with those before treatment, the RR, partial pressure of carbon dioxide in arterial blood(PaCO₂), PCT, APACHE Ⅱ scores and SOFA scores were significantly decreased(P<0.05), while the partial pressure of oxygen in arterial blood(PaO₂) and oxygenation indexes were significantly increased in the HFNC group and the NIPPV group after treatment(P<0.05), but the above indicators were not significantly different between the two groups before and after treatment(P>0.05). During treatment, there were no significant differences in the 72-hour fluid balance volume, non-invasive mechanical ventilation time, invasive mechanical ventilation rate and invasive mechanical ventilation time between the two groups(P>0.05). The results of univariate analysis showed that there were statistically significant differences in the RR, oxygenation index, APACHE Ⅱ scores, SOFA scores, and 72-hour fluid balance volume between the HFNC treatment success group and the HFNC treatment failure group at admission(P<0.05). The results of multivariate Logistic regression analysis showed that higher level of RR at admission and 72-hour fluid balance volume were the risk factors of failure of HFNC in treatment of AECOPD patients(P<0.05). Conclusion　Both HFNC and NIPPV treatments can effectively improve RR and blood gas indexes in patients with AECOPD complicated with type Ⅱ respiratory failure. Higher level of RR at admission and 72-hour fluid balance volume are the risk factors of failure of HFNC in treatment of AECOPD patients.]]> 2020/11/3 8:38:30 LU Hu-qiang, WANG Fang, WANG Jing-cheng, et al. 6true ［Abstract］　Objective　To observe the therapeutic effects of Speeder Board manipulation combined with Ashi ultrasonic therapeutic apparatus on chronic ankle sprain. Methods　Sixty-three patients who were diagnosed with chronic ankle sprain were selected from the Department of Outpatient and the Department of Emergency of the People′s Hospital of Guangxi Zhuang Autonomous Region, and were divided into two groups according to the random number table method. The control group(n=31) received routine massage manipulation, while the observation group(n=32) was treated with Speeder Board manipulation combined with Ashi ultrasonic therapeutic apparatus. The two groups were treated three times a week, and a total of 10 treatments were performed throughout the course. The thickness of the anterior talofibular ligament and the calcaneofibular ligament on the affected side and the healthy side of the ankle joint were measured by musculoskeletal ultrasound before and after treatment, and a follow-up of 6 months after treatment. The therapeutic effect was evaluated. Results　The American Orthopaedic Foot & Ankle Society(AOFAS) ankle-hindfoot scores in the observation group were significantly higher than those in the control group after treatment(P<0.05). The improvements of the thickness of the anterior talofibular ligament and the calcaneofibular ligament in the observation group were better than those in the control group(P<0.05). Conclusion　The long-term effect of Speeder Board manipulation combined with Ashi ultrasonic therapeutic apparatus is better than that of the routine massage manipulation on chronic ankle sprain, and the combined therapy is worthy of popularization and application.]]> 2020/11/3 8:38:30 LIANG Bin, LIAN Zhan-xiong, WANG Cheng, et al. 5true ［Abstract］　Objective　To observe the curative effect of the digital subtraction angiography(DSA)-guided radiofrequency for posterior ramus of lumbar spinal nerve combined with needle knife in treatment of lumbar facet related lumbago. Methods　Seventy-two patients with lumbar facet related lumbago who received treatment in our hospital from August 2018 to August 2019 were selected and divided into control group and observation group by the random number table method, with 36 cases in each group. The control group was treated with DSA-guided radiofrequency for posterior ramus of lumbar spinal nerve, and the observation group received DSA-guided radiofrequency for posterior ramus of lumbar spinal nerve combined with needle-knife therapy. Visual Analogue Scale(VAS) scores and Oswestry Disability Index(ODI) scores were compared between the two groups before and 1 day, 3 days, 7 days, 1 month, 3 months after operation. The curative effect and the incidence of postoperative complications were compared between the two groups. Results　The postoperative VAS scores and ODI scores showed a downward trend in both groups. The VAS scores and the ODI scores in the two groups at each time point after operation were lower than those before operation, and the decrease ranges of the observation group were significantly greater than those of the control group(P<0.05). There were no significant differences in the VAS scores and the ODI scores between the two groups before operation, and 1 day, 3 days and 7 days after operation(P>0.05). The VAS scores and the ODI scores in the observation group were lower than those in the control group 1 and 3 months after operation, and the differences were statistically significant(P<0.05). After 6 months of treatment, the excellent and good rate of the observation group was 88.89%, and that of the control group was 63.89%, with a significant difference between the two groups(P<0.05). Conclusion　DSA-guided radiofrequency for posterior ramus of lumbar spinal nerve combined with needle knife has a significant effect on treatment of lumbar facet related lumbago. The patients recover well in activity function after operation, and no obvious adverse reactions occur, which is worthy of clinical recommendation.]]> 2020/11/3 8:38:30 YUAN Han-wu, TIAN Wen-hua, DENG Zhi-yuan, et al. 4true ［Abstract］　Objective　To survey the refractive status of primary school students in urban and rural areas of Heilongjiang Province, and to provide reference for the development of eye health intervention program for the primary school students in the province. Methods　A random cluster sampling method was adopted to include 1 306 urban primary school students(2 612 eyes) as the urban group, and 286 primary school students(572 eyes) in villages and towns as the township group. All the study subjects underwent an ophthalmic examination, including visual acuity, routine ocular examination, and optometry. The visual acuity, poor eyesight rate and refractive status were compared between the two groups. Results　With the increase of age, the poor eyesight rates in the urban group and the township group showed an increasing trend, but the change range of the poor eyesight rate in the township group was smaller. The poor eyesight rates of the left and right eyes in the urban groups of grade 2, grade 3, grade 4 and grade 5 primary school students were significantly higher than those in the township groups of grade 2, grade 3, grade 4 and grade 5 primary school students(P<0.05). There was no significant difference in the poor eyesight rate of the right eye between the urban group of grade 1 and the township group of grade 1(P>0.05), but the poor eyesight rate of the left eye in the urban group of grade 1 was significantly higher than that in the township group of grade 1(P<0.05). There was statistically significant difference between the two groups in the refractive status of the left and right eyes(P<0.05). The emmetropia rates of the left and right eyes in the township group were higher than those in the urban group, and myopia was less in the township group. Conclusion　The poor eyesight rates of city and township primary school students increase with age, and the risk of poor eyesight of city primary school students is higher.]]> 2020/11/3 8:38:30 SONG Han, CHEN Xi-hui, ZHU Rui-xi 3true ［Abstract］　Objective　To observe the short-term curative effect of selective calcium channel blocker adjuvant treatment on ulcerative colitis. Methods　One hundred patients with ulcerative colitis admitted to our hospital from February 2015 to June 2016 were selected and divided into observation group and control group by the random number table method, with 50 cases in each group. The control group was given mesalazine enteric-coated tablets, 1.0 g per time, 3 times daily, and the patients with Mayo score ≥7 points were added with live bifidobacterium oral powder and prednisone acetate tablets. The observation group received the same treatment as the control group plus nimodipine tablets, a selective calcium channel blocker. Both of the two groups were treated for 6 weeks. After the patients entered the remission period, the drug dosages were adjusted. Both of the two groups were given mesalazine enteric-coated tablets, 0.5 g per time, 3 times daily for maintenance treatment of 3 months. The level of erythrocyte sedimentation rate(ESR), the level of C-reactive protein(CRP), Mayo score, Geboes Index score, colonic mucosa score and clinical efficacy were compared between the two groups. Results　After 6 weeks of treatment, the clinical remission rate of the observation group was higher than that of the control group, and the observation group began to get remission faster than the control group, and the differences were statistically significant between the two groups(P<0.05). Three months after treatment, the Mayo scores, Geboes Index scores and colonic mucosal score of the observation group were significantly lower than those of the control group(P<0.05), and the levels of ESR and CRP of the observation group were also significantly lower than those of the control group(P<0.05). During 6 months of the follow-up, the relapse rate of the observation group was significantly lower than that of the control group(P<0.05). Conclusion　Adjuvant treatment with selective calcium channel blocker can induce remission of ulcerative colitis, improve the treatment effect, and reduce the recurrence rate.]]> 2020/11/3 8:38:30 DONG Yong, FANG Xiao-he, WU Li-ping, et al. 2true ［Abstract］　Objective　To observe the effects of a single epidural injection of morphine and dezocine on postoperative patient controlled epidural analgesia(PCEA) after cesarean section. Methods　Ninety cases of cesarean section women admitted to our hospital from January 2017 to December 2019 were selected and randomly divided into morphine group, dezocine group and control group by the random number table method, with 30 cases in each group. When the skin was sutured at the end of the operation, the morphine group, the dezocine group and the control group were injected with morphine 2 mg, dezocine 5 mg and normal saline 5 ml through the epidural cavity, respectively, and PCEA was performed on the three groups after the operation. The Visual Analogue Scale(VAS) scores were compared among the three groups 2 hours(T₁), 4 hours(T₂), 8 hours(T₃), 12 hours(T₄), 24 hours(T₅) and 36 hours(T₆) after the operation. The number of postoperative self-controlled compressions, the occurrence of adverse reactions including nausea, vomiting, skin itching, dizziness, respiratory depression, lower limb sensation and motor abnormalities were compared among the three groups. Results　The VAS scores showed an upward trend and then a downward trend in the three groups. At T₁, T₂ and T₃ time points, the VAS scores in the control group were significantly higher than those in the morphine group and the dezocine group(P<0.05), while at T₂ and T₃ time points, the VAS scores in the dezocine group were significantly higher than those in the morphine group(P<0.05). At T₄ time point, the VAS scores in the morphine group were significantly lower than those in the dezocine group and the control group(P<0.05). The number of self-controlled compressions in the control group was significantly higher than that in the morphine group and the dezocine group(P<0.05), but there was no significant difference in the number of self-controlled compressions between the morphine group and the dezocine group(P>0.05). Nausea and vomiting occurred in 6 cases(20.00%) in the morphine group after the operation, and the incidence of nausea and vomiting in the morphine group was significantly higher than that in the dezocine group(0.00%) and the control group(0.00%)(P<0.05). However, there were no significant differences among the three groups in terms of skin itching, dizziness, respiratory depression, lower limb sensation and motor abnormalities, and other adverse reactions(P>0.05). Conclusion　A single epidural injection of morphine and dezocine can significantly enhance the effects of PCEA after cesarean section. Although dezocine is slightly weaker than morphine in analgesic duration and analgesic effect, it is safer.]]> 2020/11/3 8:38:30 LIAO Yan-ying, WEI Ning, WEI Shan-shan 1true