［Abstract］　Objective　To analyze the clinical effect of electric coagulation closure or fistulectomy assisted by electronic gastroscope on treatment of branchial fistula or cyst. Methods　Twenty patients with branchial fistula or cyst admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region from March 2018 to April 2021 were selected. The patients were divided into the observation group(electric coagulation closure or fistulectomy assisted by electronic gastroscope) and the control group(traditional cervical lymph node dissection) according to different treatment methods, with 10 cases in each group. The operation time, intraoperative blood loss, hospital stay, as well as postoperative complications and recurrence were compared between the two groups. Results　The operation time of the observation group was shorter than that of the control group［65.50(36.75, 113.25)min vs 150.00(75.00, 231.00)min］, and the intraoperative blood loss in the observation group was less than that in the control group［5.00(1.75, 10.25)ml vs 23.50(20.00, 57.50)ml］, and the differences were statistically significant between the two groups(P<0.05). There was 1 case of postoperative recurrence in the control group and there was 1 case of postoperative infection in the observation group. Conclusion　Electric coagulation closure or fistulectomy assisted by electronic gastroscope can shorten the operation time and reduce the amount of intraoperative blood loss in the treatment of branchial fistula or cyst, and the treatment is effective.]]> 2021/9/30 20:36:22 LI Rong-rong, QU Shen-hong, YE Lin-song, et al. 649true ［Abstract］　Objective　To analyze the clinical characteristics of Crohn′s disease(CD) complicated with perianal lesions in children. Methods　The clinical data of 38 CD children admitted to the Department of Gastroenterology, Children′s Hospital Affiliated to Zhengzhou University from January 2015 to January 2020 were selected and divided into perianal lesion group(15 cases) and non-perianal lesion group(23 cases) according to their different perianal lesions. The incidence of complications, laboratory test results, endoscopy results, and Pediatric Crohn′s Disease Activity Index(PCDAI) scores were compared between the two groups. Results　Among the 38 CD children, 15 cases(39.45%) were complicated with perianal lesions, including 6 cases of anal fistula, 7 cases of perianal abscess, 1 case of skin tag, and 1 case of rectovaginal fistula. The age and the level of serum albumin(ALB) of the perianal lesion group were lower than those of the non-perianal lesion group, and the PCDAI scores of the perianal lesion group were higher than those of the non-perianal lesion group at admission, and there were significant differences between the two groups(P<0.05). There were no significant differences in gender, C-reactive protein(CRP), erythrocyte sedimentation(ESR), and lesion type between the two groups(P>0.05). Complications occurred in 5 cases(33.33%) in the perianal lesion group, and 9 cases(39.13%) in the non-perianal lesion group, and the difference was not statistically significant(P=0.746). Conclusion　Young male children with CD are more likely to be complicated with perianal lesions. Clinicians should pay attention to this type of child patients and improve the examinations for them.]]> 2021/9/30 20:36:22 GUO Ya-qiong, YU Jing, GAO Ruo-fei, et al. 648true ［Abstract］　Objective　To analyze the etiological composition of hypertensive inpatients and their concurrent metabolic abnormalities so as to provide reference for prevention and treatment of hypertension. Methods　The clinical data of 5 773 hypertensive patients admitted to the First Affiliated Hospital of Guangxi Medical University from January 2013 to October 2018 were retrospectively analyzed, and their etiological composition and metabolic abnormalities were analyzed. Results　Among the 5 773 hospitalized patients with hypertension, 4 376 patients(75.80%) had essential hypertension(EH) and 1 397 patients(24.20%) had secondary hypertension(SH). Among the SH patients, the patients with primary aldosteronism accounted for the highest proportion(46.03%), followed by those with renal parenchymal hypertension(43.88%). The proportion of the males and the patients with a family history of hypertension in the EH group was significantly greater than that in the SH group(P<0.05); the patients in the SH group were younger than those in the EH group. The systolic blood pressure and the diastolic blood pressure in the SH group were higher than those in the EH group on admission, and the differences were statistically significant(P<0.05). The abnormal rates of lipid metabolism and glucose metabolism in the EH group were significantly higher than those in the SH group(P<0.05), but the incidence of hyperuricemia in the EH group was significantly lower than that in the SH group(P<0.05). Conclusion　EH is still the main cause of hypertension, but SH also accounts for an important proportion. Hypertension is often associated with a variety of metabolic abnormalities, but if SH can be diagnosed and treated early, metabolic abnormalities can be treated in time to avoid later complications, to which clinicians should pay enough attention and early screening should be targeted.]]> 2021/9/30 20:36:22 LIU Yu-ping, QIN Miao, YANG Hai-yan, et al. 647true ［Abstract］　Objective　To investigate the relationship between computed tomography(CT) imaging findings of pulmonary cryptococcosis and the pathients′ immune statuses. Methods　The clinical data of 48 patients with pulmonary cryptococcosis in Peking Union Medical College Hospital from January 2014 to September 2019 were retrospectively analyzed, and the patients were divided into the normal immune group(23 cases) and the impaired immune group(25 cases) according to whether the immune status was normal. The differences in the distribution of lesions on CT, classification and various accompanied signs were compared between the two groups, and the history of exposure to cryptococcus and clinical manifestations were analyzed. Results　Cough and expectoration were the common symptoms of pulmonary cryptococcosis. The patients in the impaired immune group had a higher fever rate than those in the normal immune group(P<0.05). The peripheral distribution and the diffuse distribution of lesions in the lungs were more common in pulmonary cryptococcosis. The proportion of the peripheral distribution of lesions on CT in the normal immune group was higher than that in the impaired immune group, and the proportion of the diffuse distribution in the normal immune group was lower than that in the impaired immune group, and the differences were significant between the two groups(P<0.05). The proportion of nodules and masses in the CT findings in the normal immune group was higher than that in the impaired immune group, while the proportion of the consolidation and mixed lesions in the normal immune group were lower than those in the impaired immune group(P<0.05). Among the various concomitant signs on CT, halo sign, air bronchogram and cavity were the more common concomitant signs in pulmonary cryptococcosis. Among these concomitant signs, the proportion of the air bronchogram in the impaired immune group was higher than that in the normal immune group(P<0.05). Conclusion　CT findings of pulmonary cryptococcosis are closely related to the patients′ immune statuses. However, there are some similarities in nodule margin and enhancement manners for the pulmonary cryptococcosis patients with different immune statuses. Identifying these similarities and differences can help to make early diagnosis of pulmonary cryptococcosis.]]> 2021/9/30 20:36:22 HUANG Yao, SUI Xin, SONG Wei 646true ［Abstract］　Objective　To explore the effect of cerebral collateral circulation on the efficacy and prognosis of intravenous thrombolysis in patients with acute cerebral infarction. Methods　The medical records of 121 patients with acute cerebral infarction who received intravenous thrombolytic therapy and cerebral vascular digital subtraction angiography(DSA) examination in Liuzhou People′s Hospital from January 2018 to December 2019 were selected. According to the results of DSA, the patients were divided into good collateral circulation compensation group(grade 3～4, 53 cases) and poor collateral circulation compensation group(grade 0～2, 68 cases). The cerebral infarction volume, National Institute of Health Stroke Scale(NIHSS) score, Activities of Daily Living(ADL) score and the incidence of cerebral hemorrhage were compared between the two groups after thrombolysis. Results　There were 4 cases(7.55%) of bleeding complications in the good collateral circulation compensation group after thrombolysis, and 15 cases(22.06%) of bleeding complications in the poor collateral circulation compensation group, and the difference between the two groups was statistically significant(χ²=4.739, P=0.029). The cerebral infarction volume in the good collateral circulation compensation group was significantly smaller than that in the poor collateral circulation compensation group after thrombolysis［10.00(8.00,12.80)cm³ vs 37.00(28.25,44.25)cm³, P<0.05］. After thrombolysis, the NIHSS scores showed a downward trend and the ADL scores showed an upward trend in the two groups, and the changing ranges of the good collateral circulation compensation group were significantly greater than those of the poor collateral circulation compensation group(P<0.05). Conclusion　Cerebral collateral circulation has an important impact on the efficacy and prognosis of intravenous thrombolysis in patients with acute cerebral infarction. Good cerebral collateral circulation can significantly improve the prognosis of the patients.]]> 2021/9/30 20:36:22 HUANG Guang-jian, GAO Wen, QIN Hui-xun, et al. 645true ［Abstract］　Objective　To observe the effect of heated and oxygenated ultrasonic atomization on acute exacerbation of chronic obstructive pulmonary disease(AECOPD). Methods　Sixty patients with AECOPD who were admitted to Jiangbin Hospital of Guangxi Zhuang Autonomous Region from April 2018 to August 2019 were selected and divided into a control group and an observation group by random number table method, with 30 cases in each group. The control group received conventional oxygen atomization inhalation therapy, while the observation group was treated with heated and oxygenated ultrasonic atomization. The course of treatment for both groups was 1 week. The Chronic Obstructive Pulmonary Disease Assessment Test(CAT) scores, arterial partial pressure of oxygen(PaO₂), arterial partial pressure of carbon dioxide(PaCO₂) and the forced expiratory volume in one second to predicted value ratio(FEV₁%pred) were compared between the two groups before and after treatment. Results　Before treatment, there were no significant differences between the two groups in CAT scores, PaO₂, PaCO₂, and FEV₁%pred(P>0.05). After treatment, the CAT scores and PaCO₂ of the observation group were lower than those of the control group, and PaO₂ and FEV₁%pred of the observation group were higher than those of the control group, and the differences were statistically significant(P<0.05). Conclusion　Compared with conventional oxygen atomization, heated and oxygenated ultrasonic atomization has better curative effect on AECOPD.]]> 2021/9/30 20:36:22 CHEN Cai-ming, JING Qing-ling, ZHENG Li, et al. 644true ［Abstract］　Objective　To analyse the clinical efficacy of ultrasound-guided low-temperature plasma radiofrequency ablation in treatment of lumbar arthrogenic low back pain. Methods　Sixty patients with lumbar arthrogenic low back pain were selected from August 2019 to August 2020 and were randomly divided into control group(30 cases) and observation group(30 cases). The control group was treated with ultrasound-guided nerve block of posterior ramus of lumbar spinal nerves for lumbar arthrogenic low back pain. The observation group received ultrasound-guided low-temperature plasma radiofrequency ablation in posterior ramus of lumbar spinal nerves for treatment of lumbar arthrogenic low back pain. The Visual Analogue Scale(VAS) scores and Oswestry Disability Index(ODI) scores of the patients were recorded before treatment and 1 day, 7 days, one month and three months after treatment. The clinical efficacy was compared between the two groups 6 months after treatment. Results　Compared with those before treatment, the VAS scores and ODI scores in the two groups were decreased significantly 1 day after treatment(P<0.05). However, the VAS scores and ODI scores in the observation group were significantly lower than those in the control group 7 days, one month and three months after operation, and the differences were significant(P<0.05). After 6 months, the excellent and good rate of the observation group(83.33%) was significantly higher than that of the control group(30.00%), and the difference was significant(P<0.05). Conclusion　Ultrasound-guided low-temperature plasma radiofrequency ablation in posterior ramus of lumbar spinal nerves is effective for treatment of lumbar arthrogenic low back pain, and it is worthy of clinical application.]]> 2021/9/30 20:36:22 YANG Fan, TIAN Wen-hua, DENG Zhi-yuan 643true ［Abstract］　Objective　To compare the clinical efficacy of low-dose intravenous gamma globulin in the treatment of children with typical and incomplete Kawasaki disease. Methods　From February 2018 to May 2020, 86 children with Kawasaki disease who were admitted to Lianjiang People′s Hospital were selected and divided into incomplete Kawasaki disease group(incomplete group, 52 cases) and typical Kawasaki disease group(typical group, 34 cases) according to different types of the disease. Both groups were treated with conventional treatments(including nebulization for reducing phlegm, physical cooling and nutritional support). On this basis, low-dose intravenous infusion of gamma globulin(1 g/kg)+oral aspirin was used. The length of hospital stay, blood indexes, and the incidence of coronary artery disease were compared between the two groups. Results　The length of hospital stay in the incomplete group was (8.07±0.46)days, and that in the typical group was (10.70±0.39)days, and the difference was statistically significant between the two groups(t=27.486, P=0.000). After treatment, the levels of C-reactive protein(CRP), platelets, white blood cells, and erythrocyte sedimentation in the incomplete group were significantly lower than those in the typical group(P<0.05). Compared with those before treatment, the incidence rates of coronary artery disease in the two groups decreased significantly after treatment(P<0.05), and the incidence of coronary artery disease in the incomplete group was significantly lower than that in the typical group(P<0.05). Conclusion　The effect of low-dose intravenous gamma globulin on the treatment of children with incomplete Kawasaki disease is better than that of children with typical Kawasaki disease. It can shorten the hospital stay, improve blood indexes, and reduce the incidence of coronary artery disease.]]> 2021/9/30 20:36:22 HE Li-ying, YE Hong 642true ［Abstract］　Objective　To explore the effect of pharmaceutical interventions on rational use of Traditional Chinese Medicine injections. Methods　One hundred and sixty patients who received treatment of Traditional Chinese Medicine injections in the People′s Hospital of Napo County from January 2019 to December 2019 were selected and divided into an observation group and a control group according to the random number table method, with 80 cases in each group. The control group was treated with conventional Traditional Chinese Medicine injections, and the observation group received the same conventional treatment of Traditional Chinese Medicine injections plus pharmaceutical interventions. The rationality of medication, hospitalization expenses, costs of Traditional Chinese Medicine injections, defined daily doses(DDDs) and adverse reactions were compared between the two groups. Results　In terms of incompatibility, combined medication, course of treatment, drug usage and dosage and indications, the rational rates of medication in the observation group were significantly higher than those in the control group(P<0.05). The hospitalization expenses, costs of Traditional Chinese Medicine injections and drug DDDs of the observation group were significantly lower than those of the control group(P<0.05). The incidence of adverse reactions in the observation group was significantly lower than that in the control group(P<0.05). Conclusion　Pharmaceutical interventions can improve the rational utilization rate of Traditional Chinese Medicine injections, reduce the treatment costs, and improve the safety of drug use, which is worthy of clinical promotion.]]> 2021/9/30 20:36:22 XU Lin, HUANG Chun-chun, WANG Ning-li, et al. 641true ［Abstract］　Objective　To explore the predictive value of neutrophil-to-lymphocyte ratio(NLR), serum procalcitonin(PCT) and lactic acid levels in risk stratification of community-acquired pneumonia(CAP) patients. Methods　A total of 282 elderly patients diagnosed with CAP in Guangzhou Hospital of Integrated Traditional Chinese and Western Medicine from January 2018 to December 2019 were selected as the study subjects. According to different CURB65 scores, the patients were divided into a low-risk group(192 cases) with CURB65 scores ≤2 points, and a high-risk group(90 cases) with CURB65 scores >2 points. After blood samples were collected, blood cells were counted and NLR value was calculated. Meanwhile, PCT and lactic acid levels in the patients′ peripheral blood were detected. Receiver operating characteristic(ROC) curve was used to compare the effectiveness of NLR, PCT and lactic acid in diagnosis of CAP disease risk stratification. Results　The detection results of NLR, PCT and lactic acid in the low-risk group were significantly lower than those in the high-risk group(P<0.05). The cut-off value of NLR for distinguishing high-risk CAP(severe pneumonia) was 13.10, and the area under curve(AUC) was 0.88. The cut-off value of serum lactic acid for predicting high-risk CAP was 2.05 mmol/L, and the AUC was 0.89. The cut-off value of PCT for predicting high-risk CAP was 1.25 ng/ml, and the AUC was 0.95, and the sensitivity and specificity were 84.40% and 94.34%, respectively, with the Yorden index being 0.79. The three indicators had better prediction effects on the disease risk of CAP patients, and PCT was the best independent predictive factor. Conclusion　NLR, PCT and lactic acid are better biomarkers for predicting the disease stratification of CAP, among which PCT is the best indicator.]]> 2021/9/30 20:36:23 ZENG Xiao-han 640true ［Abstract］　Objective　To analyze the clinical effects of double-incision trabeculectomy combined with phacoemulsification and intraocular lens implantation on treatment of primary angle-closure glaucoma with cataract. Methods　The clinical data of 112 patients undergoing surgical treatment in Zhengzhou Central Hospital Affiliated to Zhengzhou University due to primary glaucoma with cataract from August 2017 to August 2019 were retrospectively analyzed. The patients were divided into observation group(62 cases, 67 eyes) and control group(50 cases, 54 eyes) according to different surgical methods. The observation group was given combined surgery of double-incision phacoemulsification+aspheric intraocular lens implantation+trabeculectomy. The control group was given combined surgery of same incision phacoemulsification+aspheric intraocular lens implantation+trabeculectomy. The visual acuity and intraocular pressure(best corrected visual acuity, intraocular pressure value), central anterior chamber depth and angle opening degree, average area and density of corneal endothelial cells, postoperative filtering bleb formation and the occurrence of complications were compared between the two groups before surgery and 6 months after surgery. Results　Compared with those before surgery, the best corrected visual acuity was significantly improved(P<0.05), while the intraocular pressure was significantly decreased(P<0.05) in the two groups 6 months after surgery, but there were no significant differences between the two groups after surgery(P>0.05). Compared with those before surgery, the central anterior chamber depth and angle opening degree in the two groups were significantly higher(P<0.05), and the two indexes in the observation group were significantly higher than those in the control group(P<0.05). Compared with those before surgery, the average areas of corneal endothelial cells in the two groups were increased, and the area of the observation group was significantly larger than that of the control group. Compared with those before surgery, the densities in the two groups were decreased, and the density of the observation group was significantly lower than that of the control group(P<0.05). The functional filtering bleb formation rate in the observation group was significantly higher than that in the control group(P<0.05), and there was no statistically significant difference in the incidence of postoperative complications between the two groups(P>0.05). Conclusion　Both the same incision and double-incision procedures of trabeculectomy combined with phacoemulsification and intraocular lens implantation are safe and effective for treatment of primary glaucoma with cataract. However, double-incision surgery can reduce the loss of corneal endothelial cells and is more conducive to the formation of postoperative functional filtering bleb.]]> 2021/9/30 20:36:23 LIU Xi 639true ［Abstract］　Objective　To explore the expressions of Golgi phosphoprotein 3(GOLPH3) and vascular endothelial growth factor(VEGF) in lung adenocarcinoma and their clinical significances. Methods　The clinicopathological data of 66 patients with lung adenocarcinoma who underwent surgical treatment from January 2015 to June 2017 in Wuxi Clinical College of Anhui Medical University were collected to analyze the relationship between the expressions of GOLPH3 and VEGF and clinicopathological data and the patients′ prognosis. Results　The positive expression rates of GOLPH3 and VEGF in lung adenocarcinoma tissues were significantly higher than those in paracancerous tissues(P<0.05). Cox regression analysis showed that TNM staging(HR=2.110, 95%CI: 1.048-4.250, P<0.05), high expression of VEGF(HR=2.136, 95%CI: 1.039-4.391, P<0.05) and high expression of GOLPH3(HR=2.239, 95%CI: 1.075-4.661, P<0.05) were the independent risk factors affecting the patients′ survival rate. Conclusion　High expressions of GOLPH3 and VEGF in lung adenocarcinoma may promote tumor development and metastasis, and suggest a poorer prognosis. TNM staging, high expression of VEGF and high expression of GOLPH3 are the independent risk factors affecting the patients′ survival rate.]]> 2021/9/30 20:36:23 ZANG Zhi-yi, WEI Xiao-dong 638true ［Abstract］　Objective　To establish a method for the simultaneous determination of emodin and chrysophanol in Hemorrhoids Fistula Lotion by high-performance liquid chromatography(HPLC) and to provide the basis for the quality evaluation of the preparation. Methods　The contents of emodin and chrysophanol in Hemorrhoids Fistula Lotion were simultaneously determined by HPLC. A chromatographic column of Eclipse XDB-C18(4.6 mm×250 mm, 5 μm) was used. Methanol-0.1% phosphoric acid aqueous solution(80∶20, V/V) was used as mobile phase at a flow rate of 0.8 ml/min, and the detection wavelength was set at 254 nm. The column temperature was 25 ℃ and the injection volume was 10 μl. Results　There was a good linear relationship between emodin injection volume and its peak area in a range of 1.90～6.00 μg/ml(n=5, R=0.999), and there was a good linear relationship between chrysophanol injection volume and its peak area in a range of 4.00～18.51 μg/ml(n=5, R=0.999), with the average recovery rates of emodin and chrysophanol being 98.80% and 102.50%, respectively, and the relative standard deviations(RSD) of emodin and chrysophanol being 0.19%(n=6) and 0.02%(n=6), respectively. Conclusion　The method has the advantages of simple operation, accuracy, good recovery rate, high precision and good repeatability, and rapid analysis. It can be used for the quality control method of Hemorrhoids Fistula Lotion.]]> 2021/9/30 20:36:23 MENG Li-bin, PAN Hong-ping 637true ［Abstract］　Objective　To construct the 3-phosphoinositide dependent protein kinase-1(PDK1) gene short hairpin ribonucleic acid(RNA)(shRNA) lentiviral vector, and to identify its interference effect on the PDK1 gene of prostate cancer cells(PC-3). Methods　The PDK1 gene sequence was searched in GenBank database, and the PDK1 specific over-interference sequence was designed according to the sequence. The LV3 vector was linearized for overexpression using restriction enzymes, and the linearized products were purified and the directed ligation was conducted. The recombinant interfering virus plasmid and the lentivirus packaging plasmid were co-transfected into 293T cells, and the virus titer was determined. The constructed lentivirus was infected with PC-3 cells, and the PDK1 gene interference effect was identified by real-time reverse transcription polymerase chain reaction(real-time RT-PCR) method. Results　The sequencing results confirmed that the PDK1 gene RNA interference(RNAi) lentiviral vector was successfully constructed, and the lentiviral titer was 1×10⁸ TU/ml. The results of real-time RT-PCR showed that LV3-PDK1-shRNA lentivirus infection of PC-3 cells could significantly inhibit the expression of PDK1 mRNA in PC-3 cells(P<0.05). Conclusion　The PDK1 gene RNAi lentiviral vector is successfully constructed, and the PC-3 cell line stably silencing the PDK1 gene is obtained, which lays a foundation for the future studies of the role of PDK1 gene in prostate cancer cell models.]]> 2021/9/4 10:38:09 HAO Nan, NONG De-yong, LI Zuan, et al. 636true ［Abstract］　Objective　To explore the effect of erythromycin on the osteogenesis of MC3T3-E1 cells stimulated by polymethyl methacrylate(PMMA) wear particles and its potential mechanisms. Methods　MC3T3-E1 cells were pretreated with erythromycin at different concentrations(0.0 μg/ml, 0.2 μg/ml, 1.0 μg/ml, 5.0 μg/ml and 10.0 μg/ml), and then PMMA wear particles were added for intervention and stimulation. After 72 hours, the proliferation activity and osteogenic activity of MC3T3-E1 cells were detected. Real-time quantitative polymerase chain reaction(RT-PCR) was used to detect the expression levels of RUNT related transcription factor 2(Runx2), osteogenesis-related transcription factor Osterix, osteocalcin(OCN) and p65 in MC3T3-E1 cells. Results　Compared with that of the blank control group, the alkaline phosphatase concentration of the PMMA-treated group was significantly decreased(P<0.05). After the intervention of erythromycin, the intracellular alkaline phosphatase concentration increased in a dose-dependent manner. Compared with those in the blank control group, the expression levels of Runx2, Osterix and OCN in the PMMA group were significantly decreased(P<0.05), while the expression level of p65 in the PMMA group was significantly increased(P<0.05). After treatment with erythromycin at different concentrations(0.2 μg/ml, 1.0 μg/ml, 5.0 μg/ml and 10.0 μg/ml), the p65 expression level of MC3T3-E1 cells was significantly lower than that of PMMA group(P<0.05). After treatment with erythromycin at different concentrations(1.0 μg/ml, 5.0 μg/ml and 10.0 μg/ml), the expression levels of Runx2 and OCN in MC3T3-E1 cells were significantly higher than those in the PMMA group(P<0.05). After treatment with erythromycin at different concentrations(5.0 μg/ml and 10.0 μg/ml), the expression level of Osterix in MC3T3-E1 cells was significantly higher than that in the PMMA group(P<0.05). The recoveries of Runx2, Osterix, OCN and p65 expression levels intervened by erythromycin were dose-dependent. Conclusion　Erythromycin can effectively improve the inhibitory effect of PMMA wear particles on the osteogenic activity of MC3T3-E1 cells, which may be achieved by down-regulating the NF-κB signaling pathway.]]> 2021/9/4 10:38:09 LIU Zi-ge, ZHANG Chen, SONG Guo-rui, et al. 635true ［Abstract］　Objective　To analyze the correlation between tongue image features and Traditional Chinese Medicine(TCM) syndromes in patients with atopic dermatitis(AD). Methods　A total of 130 AD outpatients from the dermatology clinic of the First Affiliated Hospital of Guangxi University of Chinese Medicine during March 2018 and October 2020 were selected as the case group, and 40 healthy subjects receiving physical examination were selected as the control group during the same period. The tongue images of the research subjects were collected, and the red-green-blue(RGB) value and the hue(H) value of hue-saturation-intensity(HSI) of the tongue images were obtained. The correlation between these feature values and TCM syndromes was analyzed. Results　The 130 AD patients included 49 cases(37.69%) of spleen-deficiency and dampness-storage type,45 cases(34.62%) of damp-heat accumulation type,and 36 cases(27.69%) of blood-deficiency and wind-dryness type. The R component values of the blood-deficiency and wind-dryness type group, the spleen-deficiency and dampness-storage type group and the damp-heat accumulation type group were significantly lower than those of the control group(P<0.05). The R component value and B component value of the blood-deficiency and wind-dryness type group were greater than those of the spleen-deficiency and dampness-storage type group and the damp-heat accumulation type group, and the differences were statistically significant(P<0.05). The H value of the damp-heat accumulation type group was significantly lower than that of the control group(P<0.05). There was no statistically significant difference in the G component value among the four groups(P>0.05). Conclusion　It has certain clinical significance to use the R, G, B component values and H value of the tongue diagnosis image parameters as the objective indicators of the dialectical classification of AD.]]> 2021/9/4 10:38:09 LI Yan-fang, WU Zhi-hong 634true ［Abstract］　Objective　To investigate the relationship between systemic immune inflammatory index(SII) and postoperative recurrence of colorectal cancer(CRC) patients. Methods　The clinical data of 118 CRC patients who received postoperative chemotherapy in our hospital from January 2016 to June 2020 were retrospectively collected, including 65 males and 53 females. The patients were 27-82 years old, with an average of (61.4±11.4)years, including 69 cases of colon cancer and 49 cases of rectal cancer. Cox regression model was used to explore the factors affecting the patients′ postoperative recurrence. The receiver operator characteristic(ROC) curve method was used to analyze the efficacy of SII in predicting the patients′ postoperative recurrence, and the study subjects were divided into high SII group(SII≥655.68×10⁹/L, n=54) and low SII group(SII<655.68×10⁹/L, n=64) according to their different cut-off values. The clinical characteristics were compared between the two groups, and the difference in relapse-free survival(RFS) time between the two groups was explored by using survival analysis. Results　The results of Cox regression analysis showed that higher levels of SII and CA19-9 were the independent risk factors affecting the CRC patients′ postoperative RFS time(P<0.05). The results of ROC curve analysis showed that the cut-off value of SII in predicting postoperative recurrence was 655.68×10⁹/L, whose corresponding sensitivity and specificity were 76.67% and 86.21%, respectively. SII had the value of predicting postoperative recurrence of colorectal cancer patients(AUC=0.855, P=0.000). The proportion of the patients with Ki-67≥60% and CA19-9>39 ng/ml in the high SII group was significantly greater than that in the low SII group(P<0.05). The levels of white blood cell(WBC), neutrophil(NEU), monocyte(MON), and platelet(PLT) in the high SII group were significantly higher than those in the low SII group, and the level of lymphocyte(LYM) in the high SII group was significantly lower than that in the low SII group(P<0.05). The median RFS time was 5.6 months(95%CI: 5.1-6.1) in the high SII group, and 8.3 months(95%CI: 7.6-9.0) in the low SII group, with a significant difference between the two groups(log-rank test: χ²=29.330, P=0.000). Conclusion　SII can be used for the assessment of postoperative RFS time in CRC patients. Clinicians should pay more attention to the patients with high SII values in order to detect the recurrence as soon as possible and take the appropriate interventions.]]> 2021/9/4 10:38:09 HU Shi-qi, PAN De-jian 633true ［Abstract］　Objective　To observe the effect of strengthening muscle strength training around the hip joint on patellofemoral pain syndrome(PFPS). Methods　Sixty PFPS patients admitted to Binhaiwan Central Hospital from June 2018 to June 2019 were selected, and were divided into observation group and control group by drawing lots, with 30 cases in each group. The control group received routine physical therapy, and the observation group received muscle strength training around the hip joint on the basis of the treatment plan of the control group. The Anterior Knee Pain Scale(AKPS) scores, the Visual Analogue Scale(VAS) scores and the peak torque(PT) of knee flexion and extension were compared between the two groups before and after treatment. Results　After treatment, the AKPS scores were significantly increased, and the VAS scores were significantly decreased in the two groups compared with those before treatment(P<0.05). Compared with the control group, the observation group had higher AKPS scores and lower VAS scores after treatment, and the differences were statistically significant(P<0.05). After treatment, the PT values of knee extension and knee flexion were significantly elevated in the two groups compared with those before treatment(P<0.05). Compared with the control group, the observation group had higher PT values of knee extension and knee flexion after treatment, and the differences were statistically significant(P<0.05). Conclusion　Strengthening muscle strength training around the hip joint is beneficial to improving the movement function of the knee joint in PFPS patients, and relieves the symptoms of knee joint pain, and has a significant effect.]]> 2021/9/4 10:38:09 LI Wei-xing, ZHANG Qing-wei 632true ［Abstract］　Objective　To analyze the prevalence rate of short-chain acyl-CoA dehydrogenase deficiency(SCADD) in newborns in Zigong City, and to summarize the clinical characteristics and gene mutation characteristics of the newborns with SCADD. Methods　A total of 24 384 newborns from Zigong City who volunteered for screening of inherited metabolic diseases in Zigong City Maternal and Child Health Hospital during May 2018 and February 2021 were selected. Tandem mass spectrometry technology was used to screen and detect the acylcarnitine levels of the newborns, and those who were positive in the initial screening and reexamination were confirmedly diagnosed by urinary organic acid analysis and genetic metabolic disease gene sequencing. Results　Of the 24 384 newborns, 249 were positive at the initial screening, and 15 were positive at the reexamination, and 1 was confirmedly diagnosed with SCADD. The prevalence rate was 1/24 384. There were no obvious abnormalities in the clinical manifestations of the newborns with SCADD. The results of tandem mass spectrometry showed that blood butyryl-carnitine(C4), ratio of C4 to acetyl-carnitine(C2), ratio of C4 to propionyl-carnitine(C3) were increased. The results of urine organic acid test showed that the level of ethylmalonic acid was increased. Gene sequencing of family metabolic diseases revealed that the newborns had two SCAD gene mutations in which one was a known mutation c.1031A>G(from the mother), and the other was an unreported mutation c.293A>G(from the father). At the same time, the c.428C>T mutation of SLC22A5 gene(from the father) occurred. After the newborns were diagnosed with SCADD, they took L-carnitine and vitamin B₂ daily and were given dietary guidance. During the follow-up period, no clinical symptoms appeared in the SCADD newborns, and their physical and intellectual developments were normal. Conclusion　The prevalence rate of SCADD in newborns in Zigong City is higher than that in other regions of China. Early diagnosis and early treatment can effectively improve the prognosis of the SCADD newborns.]]> 2021/9/4 10:38:09 HU Qin, HE Yi, YE Qiang, et al. 631true ［Abstract］　Objective　To observe the effect of single syringe gas-liquid exchange on treatment of vitreous rebleeding after vitrectomy for proliferative diabetic retinopathy. Methods　The clinical data of 30 patients suffering from vitreous rebleeding after vitrectomy for proliferative diabetic retinopathy and admitted to the First Affiliated Hospital of Guangxi Medical University from January 2019 to December 2020 were selected. All the patients were in sitting or prone position, and the vitreous cavity gas-liquid exchange surgery was performed using a single syringe. The patients′ the postoperative visual acuity, lens, fundi and intraocular pressure were observed. Results　Of the 30 patients, 2 patients(6.67%) needed to receive vitreous gas-liquid exchange surgery again due to the second postoperative bleeding. Compared with that before surgery, the visual acuity of the 30 patients improved significantly after surgery, and the difference was statistically significant［(0.60±0.34)logMAR(postoperative) vs (1.47±0.52)logMAR(preoperative); t=8.267, P=0.001］. The fundi of the 30 patients could be observed clearly 14 days after surgery, and the intraocular pressure was in the normal range. Among them, 2 patients still needed to use ocular hypotensive agents after gas absorption. Conclusion　It is safe and effective to use a single syringe to perform vitreous cavity gas-liquid exchange surgery on patients suffering from vitreous rebleeding after vitrectomy for proliferative diabetic retinopathy.]]> 2021/9/4 10:38:09 ZHONG Xin, LIU Jiao-lian, LIANG Yong-shun, et al. 630true ［Abstract］　Objective　To analyze the epidemiological characteristics of emergency trauma patients and to provide scientific basis for trauma prevention and treatment. Methods　The data of 5 012 trauma patients admitted to the Department of Emergency, the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2016 to January 2020 were collected. The gender, age, traumatic time, traumatic locations, causes and severity of trauma of the patients were analyzed. Results　A total of 5 012 trauma patients were included in this study, including 3 570 males(71.23%) and 1 442 females(28.77%). Trauma occurring in 20-50 years old age group accounted for the largest proprotion, and the occupation of farmers and workers accounted for the largest proprotion. Within a year, the incidence of traumatic events was relatively high in October. Within 24 hours of a day, the most common traumatic events occurred during the period from 18:00 to 19:59, followed by 20:00 to 21:59. The limbs and pelves accounted for the highest proportion of traumatic locations, accounting for 36.97%(1 853 cases), followed by the head and neck, accounting for 20.01%(1 003 cases), the chest, accounting for 17.50%(877 cases), the abdomen, accounting for 15.52%(778 cases) and the face, accounting for 10.00%(501 cases). The most common cause of trauma was traffic injuries, followed by falls/fall injuries, and the total number of cases of traffic injuries and falls/fall injuries accounted for 56.57% of all the trauma cases. Conclusion　Young and middle-aged males account for the largest proportion of emergency trauma patients. Traumatic events occurring in the 18:00 to 21:59 period account for a relatively large proportion, and traffic injuries and falls/ fall injuries are the main causes of trauma.]]> 2021/9/4 10:38:09 LI Zhe, LYU Li-wen, SHEN Yin 629true ［Abstract］　Objective　To observe the effectiveness and safety of transumbilical single-port total laparoscopic hysterectomy in treatment of benign uterine diseases. Methods　The clinical data of 80 benign uterine disease patients receiving total laparoscopic hysterectomy in Longyan First Hospital Affiliated to Fujian Medical University from July 2019 to May 2020 were retrospectively analyzed. The patients were divided into traditional total laparoscopic hysterectomy(LTH) group and single-port laparoscopy(SPL) group according to different operation methods, with 40 cases in each group. The operative time, intraoperative blood loss, Visual Analogue Scale(VAS) scores on the first day after operation, hospital costs, postoperative hospital stay and postoperative complications were compared between the two groups. Results　There were no significant differences in intraoperative blood loss, postoperative hospital stay and postoperative complications between the two groups(P>0.05). The operative time of the SPL group was longer than that of the traditional LTH group, and the difference was statistically significant(P<0.05). The VAS scores on the first day after operation in the SPL group were lower than those in the traditional LTH group(P<0.05). The hospital costs in the SPL group were higher than those in the traditional LTH group(P<0.05). Conclusion　Transumbilical single-port total laparoscopic hysterectomy has the advantages of postoperative minimally invasive wound and less pain in treatment of benign uterine diseases, and has a good application value.]]> 2021/9/4 10:38:09 WANG Fan, WANG Jin-yong, CHEN Fu-chun, et al. 628true ［Abstract］　Objective　To investigate the value of postoperative electroencephalogram(EEG) monitoring for prognosis evaluation in patients with spontaneous supratentorial hemorrhage complicated with coma. Methods　One hundred and thirty-six patients with spontaneous supratentorial hemorrhage complicated with coma within 72 hours and receiving emergency hematoma removal surgery in the Intensive Care Unit of the Department of Neurological Diseases in the Third Affiliated Hospital of Chongqing Medical University from April 2016 to April 2020 were collected. The bedside video EEG monitoring was performed 14 days after operation and the EEG characteristics were analyzed. The patients′ prognoses were assessed by Extended Glasgow Outcome Score(GOS-E) 90 days after operation. Results　Of the 136 patients, 69 patients(50.7%) had a good prognosis and 67 patients(49.3%) had a poor prognosis. Logistic regression analysis showed that larger hematoma volume(OR=6.450, 95%CI: 3.018-13.783), higher EEG Young classification(OR=2.516, 95%CI: 1.804-3.510), EEG non-responsiveness(OR=2.167, 95%CI: 1.091-4.303), and no sleep wave(OR=3.040, 95%CI: 1.319-7.006) indicated a poor prognosis. The higher the Glasgow Coma Scale(GCS) score(OR=0.549, 95%CI: 0.242-1.246), the better the prognosis. Conclusion　EEG monitoring can be used as a reference index to evaluate the postoperative prognosis of patients with spontaneous supratentorial hemorrhage complicated with coma receiving emergency hematoma removal surgery.]]> 2021/9/4 10:38:09 DENG Chun-yan, WANG Yu-tao, LIU Jiang, et al. 627true ［Abstract］　Objective　To investigate the effects of Kang′ai injection combined with chemotherapy on clinical efficacy, immune function and quality of life in patients with advanced lung squamous cell carcinoma. Methods　A total of 94 lung squamous cell carcinoma patients who were newly treated in the Affiliated Hospital of Guangdong Medical University from July 2017 to October 2020 were collected and divided into control group(45 cases) and observation group(49 cases) according to random number table method. The control group received cisplatin plus gemcitabine chemotherapy. The observation group received the same treatment as the control group plus adjuvant therapy with Kang′ai injection. Both groups were treated for 4 courses. T cell counts before treatment and after treatment, and the clinical efficacy, quality of life score and adverse reactions of chemotherapy after treatment were compared between two groups. Results　After treatment, the objective response rate of the observation group was significantly higher than that of the control group(P<0.05), but there was no significant difference in the disease control rate between the two groups(P>0.05). The Karnofsky Performance Status(KPS) scores of the observation group were significantly higher than those of the control group after treatment(P<0.05). The total number of T lymphocytes, absolute value of T helper cells and absolute value of T suppressor cells in the observation group were significantly higher than those in the control group after treatment(P<0.05). The incidence rates of adverse reactions of nausea and vomiting, thrombocytopenia and leukopenia in the observation group were significantly lower than those in the control group(P<0.05). Conclusion　Kang′ai injection combined with chemotherapy may improve the immune function and quality of life of patients with advanced lung squamous cell carcinoma, reduces the adverse reactions of the patients, thus improves the clinical efficacy,which is worthy of clinical promotion.]]> 2021/9/4 10:38:09 HUANG Zhong, LIU Mei-lian, PENG Xiao-xia, et al. 626true ［Abstract］　Objective　To explore the expression of calcium-activated chloride channel regulator 1(CLCA1) in colorectal cancer(CRC) and its relationship with clinicopathological characteristics and prognosis, and to analyze its gene set enrichment. Methods　The data of CLCA1 messenger RNA(mRNA) expression were downloaded from the Cancer Genome Atlas(TCGA) database, and the gene set enrichment analysis of different phenotypes of CLCA1 was performed by using GSEA software. The differential expression of CLCA1 mRNA between the CRC tissues and the adjacent tissues was analyzed, and its correlation with clinicopathological characteristics was analyzed. The difference of CLCA1 between the CRC tissues and the adjacent tissues was analyzed by immunohistochemistry. Results　The expression of CLCA1 in the CRC tissues was significantly lower than that in the adjacent tissues. The immunohistochemical staining scores of the CRC tissues were significantly lower than those of the adjacent tissues［(2.46±1.09)scores vs (5.65±0.49)scores; t=6.458, P=0.000］. The level of CLCA1 mRNA expression in the adjacent tissues was significantly higher than that in the CRC tissues in clinical stage Ⅰ-Ⅱ and clinical stage Ⅲ-Ⅳ(P<0.05), and the level of CLCA1 mRNA expression in the CRC tissues in clinical stage Ⅰ-Ⅱ was significantly higher than that in the CRC tissues in clinical stage Ⅲ-Ⅳ(P<0.05). The proportion of the patients in the CLCA1 high expression group with clinical stage Ⅰ-Ⅱ, N₀ stage(N staging) and M₀ stage(M staging) was significantly greater than that in the CLCA1 low expression group(P<0.05). The survival prognosis of the CLCA1 high expression group was significantly better than that of the CLCA1 low expression group(χ²=8.200, P=0.004). The results of gene set enrichment analysis showed that 19 gene sets were significantly enriched in the CLCA1 high expression phenotypes, and that no gene sets were found to be significantly enriched in the CLCA1 low expression phenotypes. Conclusion　CLCA1 is down-regulated in the CRC tissues and is related to the patients′ clinicopathological characteristics and prognoses. It has the potential as a marker for the diagnosis, treatment and outcome prediction of CRC.]]> 2021/8/6 11:16:11 LUO Shi-bo, WU Jiang-ni, QIU Xin-ze, et al. 625true ［Abstract］　Objective　To explore the correlation between plasma interleukin(IL)-38 and effector T cell subsets in patients with coronary artery disease(CAD). Methods　One hundred and twenty CAD patients admitted to Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology from March 2019 to July 2019 were selected, including 40 patients with stable angina pectoris(SAP), 40 patients with unstable angina pectoris(UAP), and 40 patients with acute myocardial infarction(AMI), and the patients were divided into SAP group, UAP group and AMI group, respectively. In addition, other 40 patients with chest pain syndrome(CPS) during the same period were selected as the control group. The differences in the levels of inflammatory factors［IL-38, interferon(IFN)-γ, IL-4 and IL-17］, effector T cell subsets(Th1, Th2 and Th17) and other clinical data were compared among the four groups, and the correlation between IL-38 and each indicator was analysed. Results　There were statistically significant differences in the levels of total cholesterol, high-density lipoprotein cholesterol(HDL-C), fasting blood glucose, C-reactive protein(CRP), brain natriuretic peptide(BNP) and troponin I, left ventricular ejection fraction(LVEF), and the proportion of the patients receiving statins among the four groups(P<0.05). The levels of IL-38 and IL-17 in the AMI group were significantly higher than those in the SAP group(P<0.05), but there were no significant differences in the levels of IL-38 and IL-17 between the AMI group and the UAP group(P>0.05). The level of IL-4 in the SAP group was higher than that in the UAP group and the AMI group, and the level of IL-4 in the UAP group was higher than that in the AMI group, and the differences were statistically significant(P<0.05). The level of IFN-γ in the AMI group was higher than that in the UAP group and the SAP group, and the level of IFN-γ in the UAP group was higher than that in the SAP group, and the differences were statistically significant(P<0.05). The results of Pearson correlation analysis showed that plasma IL-38 level was positively correlated with Th1, Th17, IFN-γ, IL-17, fasting blood glucose, CRP, troponin Ⅰ, BNP and left ventricular end-diastolic diameter(LVEDD)(P<0.05), and negatively correlated with IL-4 and LVEF(P<0.05). Conclusion　The level of plasma IL-38 in patients with CAD is significantly increased, and is positively correlated with Th1 and Th17 immune responses.]]> 2021/8/6 11:16:11 FU Yan, YU Kun-wu, PENG Yu-dong, et al. 624true ［Abstract］　Objective　To investigate the expression changes of neutrophil gelatinase-associated lipocalin(NGAL) and matrix metalloproteinase-9(MMP-9) genes in peripheral blood mononuclear cells of patients with chronic obstructive pulmonary disease(COPD) at different stages and to analyze their clinical significances. Methods　From November 2016 to July 2017, twenty-five patients with acute exacerbation of chronic obstructive pulmonary disease(AECOPD), 80 patients with stable chronic obstructive pulmonary disease(SCOPD) and 40 healthy subjects were prospectively enrolled into the AECOPD group, the SCOPD group and the control group, respectively. According to the frequency of acute exacerbation episode(or the frequency of hospitalization) in the previous year, the patients of the SCOPD group were divided into a low-risk group(n=39) and a high-risk group(n=41). The fasting peripheral venous blood samples were collected from all the subjects in the early morning. After mononuclear cells were isolated and extracted, real-time quantitative polymerase chain reaction(RT-qPCR) technology was used to detect the expressions of NGAL mRNA and MMP-9 mRNA genes in each group and the differences in the gene expressions of NGAL mRNA and MMP-9 mRNA were compared among all the groups. At the same time, the relationship between NGAL mRNA and MMP-9 mRNA gene expressions and their correlations with clinical indicators such as Body Mass Index, Smoking Index, blood routine and lung function were analyzed. Results　(1)The relative expression levels of NGAL mRNA and MMP-9 mRNA genes in the AECOPD group were higher than those in the SCOPD group and the control group, and the relative expression levels of NGAL mRNA and MMP-9 mRNA genes in the SCOPD group were higher than those in the control group, and the differences were statistically significant(P<0.01). (2)The relative expression levels of NGAL mRNA and MMP-9 mRNA genes in the high-risk group were higher than those in the low-risk group, and the differences were statistically significant(P<0.05). (3)The relative expression levels of NGAL mRNA and MMP-9 mRNA genes in the AECOPD group and the SCOPD group were significantly positively correlated, and the correlations were statistically significant(r=0.831, P<0.01; r=0.764, P<0.01). (4)The relative expression levels of NGAL mRNA and MMP-9 mRNA genes in the SCOPD group were negatively correlated with the forced expiratory volume in one second/prediction(FEV₁%pred), and the correlations were statistically significant(r=-0.416, P<0.01; r=-0.312, P<0.05). (5)The relative expression levels of NGAL mRNA and MMP-9 mRNA genes in the SCOPD group were positively correlated with the total number of peripheral blood white blood cells, the total number of neutrophils and the percentage of neutrophils, and the correlations were statistically significant(P<0.05). The relative expression levels of these genes had no significant correlation with such clinical indexes as Body Mass Index, Smoking Index and forced expiratory volume in one second/forced vital capacity(FEV₁/FVC)(P>0.05). Conclusion　NGAL and MMP-9 may be involved in the occurrence and development of COPD, and their relative gene expression levels may become new indicators for evaluating the severity of COPD.]]> 2021/8/6 11:16:11 LI Guo, CHEN Xiao-bing, LI Gang, et al. 623true ［Abstract］　Objective　To analyze the clinical effect of modified rotary Scarf osteotomy in treatment of moderate and severe hallux valgus. Methods　Thirty-three patients(51 feet) with moderate and severe hallux valgus deformity who were hospitalized in the Department of Orthopedics and Traumatology, Beijing Miyun District Hospital of Traditional Chinese Medicine from July 2017 to July 2018 were selected as the study subjects. All the patients were treated with modified rotary Scarf osteotomy. The positions of hallux valgus angle(HVA), intermetatarsal angle(IMA) and sesamoid bone on the X-ray film were compared before and after operation, and the American Orthopedic Foot and Ankle Society(AOFAS) score and Visual Analogue Scale(VAS) score were compared before and after operation. Results　After operation, IMA and HVA were significantly lower than those before operation, and the differences were statistically significant(P<0.05). Compared with that before operation, the position of sesamoid bone was improved after operation. Compared with those before operation, AOFAS score and VAS score were significantly improved at the last follow-up. Conclusion　Modified rotary Scarf osteotomy is effective in treatment of moderate and severe hallux valgus deformity.]]> 2021/8/6 11:16:11 XI Yin-hui, ZHANG He-li, XU Hai-lin 622true ［Abstract］　Objective　To explore the application value of transthoracic echocardiography in diagnosis of DeBakey classification of acute aortic dissection. Methods　The clinical data of 71 patients with acute aortic dissection admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region from June 2015 to June 2020 were retrospectively analyzed. The consistency between emergency transthoracic echocardiography and intraoperative diagnosis for DeBakey classification of aortic dissection was analyzed. Results　The results of Kappa consistency test showed that emergency transthoracic echocardiography and intraoperative diagnosis had a good consistency in the classification of aortic dissection(Kappa=0.872, P=0.000), and the coincidence rate was 92.96%(66/71). Conclusion　Transthoracic echocardiography can diagnose DeBakey classification of acute aortic dissection more accurately, and can provide clinicians with information on the scope of lesion involvement and aortic lesions, and has an important guiding role in the selection of clinical treatments and surgical procedures.]]> 2021/8/6 11:16:11 LU Zhong-zhi, PAN Yan 621true ［Abstract］　Objective　To explore the application effects of cross suture closure method on stoma reversal. Methods　Thirty-eight patients who underwent stoma reversal in the Department of General Surgery, Yulin Red Cross Hospital from February 2019 to October 2020 were selected. The abdominal incisions were reconstructed in all the 38 patients using cross suture closure method. The operation time, intraoperative bleeding volume, postoperative pain score, postoperative hospital stay, and the incidence of incision complications were recorded. Results　The operation time was 30-85 min(50 min on average). The intraoperative bleeding volume was 15-30 ml(20 ml on average). The postoperative hospital stay was 5-14 days(8.6 days on average). The median pain scores on the first, the second, and the third day after operation were 3.5 points, 3.5 points, and 2.5 points, respectively. After operation, incision infection occurred in 2 cases(5.26%), and incision subcutaneous fat liquefaction and exudation occurred in 1 case(2.63%), and the incidence of incision complications was 7.89%(3/38). After 1 month of follow-up, there were no new cases of wound complications, and the patients were satisfied with the wound recovery. Conclusion　Cross suture closure method can reduce incision tension, promote wound healing, relieve postoperative pain and shorten the length of hospital stay, and the short-term treatment effect is remarkable.]]> 2021/8/6 11:16:11 YANG Sheng-fu, LI Xiao-he, WU Dong-bo, et al. 620true ［Abstract］　Objective　To explore the efficacy of prourokinase combined with tirofiban in prevention of no-reflow/slow-flow(NR/SF) in acute ST-segment elevation myocardial infarction(ASTEMI) patients undergoing emergency percutaneous coronary intervention(PCI). Methods　The medical records of 90 ASTEMI patients admitted to the Department of Cardiology, Zhangjiajie City People′s Hospital from September 1, 2017 to June 30, 2020 were selected. All the patients underwent emergency PCI-stent implantation. According to the intraoperative medication regimens, the patients were divided into the study group(receiving intracoronary prourokinase combined with tirofiban) and the control group(receiving tirofiban alone), with 45 cases in each group. The thrombolysis in myocardial infarction(TIMI) blood flow classification, NR/SF incidence, the levels of cardiac function indexes［including left ventricular end-systolic dimension(LVESD), fractional shortening(FS), left ventricular end-diastolic dimension(LVEDD) and ejection fractions(EF)］, the occurrence of bleeding events and adverse cardiovascular and cerebrovascular events were compared between the two groups during the period of time from preoperation until discharge. Results　The incidence of NR/SF in the study group was significantly lower than that in the control group(4.44% vs 31.11%; χ²=10.946, P=0.001). After treatment, the TIMI blood flow classification of the study group was significantly better than that of the control group(P<0.05). There were no statistically significant differences in the levels of LVEF, LVESD, EF and FS, and the incidence rates of adverse cardiac and cerebral events and accidental hemorrhage between the two groups(P>0.05). Conclusion　For ASTEMI patients undergoing emergency PCI-stent implantation, the application of prourokinase combined with tirofiban is helpful to improve blood perfusion and reduce the incidence of NR/SF, with less adverse reactions and good safety.]]> 2021/7/5 12:12:34 CHEN Li-hua, SHEN Sheng-chun, XIANG Ming-jun 619true ［Abstract］　Objective　To explore the expression levels of micro ribonucleic acid 144(miRNA-144) and micro ribonucleic acid 21(miRNA-21) in saliva and their combined detection value for the diagnosis of esophageal cancer. Methods　Eighty-five patients with esophageal cancer admitted to the Department of Cardiothoracic Surgery of Guangzhou Red Cross Hospital Affiliated to Jinan University from January 2015 to May 2019 were selected as the case group, all of whom were diagnosed by pathological biopsy and immunohistochemical examination. Thirty-two healthy volunteers were selected as the control group during the same period. The patients in both groups were older than 60 years. Polymerase chain reaction(PCR) was used to detect the expression levels of miRNA-144 and miRNA-21 in the saliva of the two groups. The receiver operating characteristic(ROC) curve method was used to evaluate the diagnostic value of the two indicators for esophageal cancer. Results　The expression levels of miRNA-144 and miRNA-21 in the saliva of the case group were significantly higher than those of the control group(P<0.05), and were related to pathological staging(P<0.05). The results of ROC analysis showed that the areas under the curve of miRNA-21 and miRNA-144 in saliva for diagnosing esophageal cancer was 0.785 and 0.777, respectively, which had diagnostic value, and the combined detection of the two indicators could further improve the diagnostic efficiency(the area under the curve was 0.851). Conclusion　miRNA-144 and miRNA-21 are highly expressed in the saliva of patients with esophageal cancer, and the combined detection of miRNA-21 and miRNA-144 has a prospect of application in screening and diagnosing esophageal cancer.]]> 2021/7/5 12:12:34 GU Jia-sheng, WU Wei-dong, LIU Qing-feng, et al. 618true ［Abstract］　Objective　To investigate the effect of intestinal microecological preparation combined with glutamine on postoperative immune function of patients with colorectal cancer. Methods　Sixty patients with colorectal cancer admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region from July 2018 to May 2019 were selected, and were divided into observation group and control group by random number table method, with 30 cases in each group. The control group received conventional routine bowel preparation(oral compound polyethylene glycol or mechanical cleaning enema) before operation, and received routine symptomatic and supportive treatment after operation. The observation group was given oral intestinal microecological preparations(Live Combined Bifidobacterium, Lactobacillus, Enterococcus and Bacillus cereus Tablets and lactulose) and glutamine every day from the third day before operation. From the first day to the seventh day after operation, in addition to the routine symptomatic and supportive treatment, oral intestinal microecological preparations(Live Combined Bifidobacterium, Lactobacillus, Enterococcus and Bacillus cereus Tablets) and glutamine were also given to the observation group. The changes of immunoglobulin A(IgA) and T-lymphocyte subpopulation levels(CD3 T cells, CD4 T cells, CD8 T cells, and CD4 T cells/CD8 T cells) were observed and compared between the two groups before operation and on the third day and the seventh day after operation. Results　The levels of IgA, CD3 T cells and CD4 T cells in the two groups showed a trend of decline and then rise after operation, and the observation group had a smaller decline and a greater increase, and the differences were statistically significant(P<0.05). The levels of CD8 T cells, and CD4 T cells/CD8 T cells in the two groups also showed a tendency to decrease first and then increase after operation, but there were no significant differences in the trends between the two groups(P>0.05). Conclusion　Combined application of intestinal microecological preparation and glutamine can effectively promote the recovery of postoperative immune function in patients with colorectal cancer.]]> 2021/7/5 12:12:34 YA Han-qing, WANG Long, LIU An-lin, et al. 617true ［Abstract］　Objective　To quantitatively analyze the root resorption of maxillary central incisor, lateral incisor and cuspid teeth in adult patients with skeletal Class Ⅱ high angle malocclusion before and after compensatory orthodontic treatment using the cone beam computed tomography(CBCT) three-dimensional reconstruction technique matching Mimics 20.0 software. Methods　Thirty adult cases of skeletal Class Ⅱ high angle malocclusion treated with orthodontic compensation in the Department of Orthodontics, Guiyang Stomatological Hospital from November 2016 to November 2018 were selected. Multiplanar reconstruction of CBCT data was performed by using Mimics 20.0 software to adjust coronal, axial and sagittal axises. The sagittal section with the long axis of the incisor edge and root tip was selected to measure the root changes of maxillary central incisor, lateral incisor and canine before and after treatment. The differences in root volume and root resorption of maxillary central incisor, lateral incisor and canine before and after treatment were compared. Results　Compared with those before treatment, the root volumes of maxillary central incisors, lateral incisors and canines were reduced after treatment, and the differences were statistically significant(P<0.05). After the orthodontic treatment, the root resorption of lateral incisors was significantly greater than that of central incisors and canines in the patients(Z=-2.783, P=0.013; Z=2.248, P=0.021), but there was no statistically significant difference between central incisors and canines in root resorption(Z=1.317, P=1.354). Conclusion　In the process of orthodontic compensation treatment for adult skeletal Class Ⅱ high angle malocclusion, the root of maxillary anterior teeth has obvious absorption. Among maxillary anterior teeth, lateral incisors have the most significant absorption, followed by central incisors, and canines have the least absorption, to which enough attention should be paid in clinical treatment.]]> 2021/7/5 12:12:34 GAO Juan, WEI Xue, ZUO Chang-yan, et al. 616true ［Abstract］　Objective　To observe the effect of butylphthalide injection combined with intelligent robot hand on treatment of hemiplegic hands in acute cerebral infarction patients and its influence on cerebral blood flow velocity. Methods　Eighty acute cerebral infarction patients with hemiplegic hands adimtted to Beiliu City People′s Hospital during March 2019 and March 2020 were selected, and were divided into study group and control group by random number table method, with 40 cases in each group. The patients in the control group were treated with conventional methods, and the patients in the study group were treated with butylphthalide sodium chloride injection and the intelligent robot hand on the basis of the treatment with the conventional methods. The National Institutes of Health Stroke Scale(NIHSS) score, Activities of Daily Living(ADL) score, maximum hand grip strength, the pressure between the fingers and cerebral blood flow were compared between the two groups before treatment, 10 days and 20 days after treatment. Results　After treatment, the NIHSS scores showed a downward trend in the two groups. The NIHSS scores of the study group were significantly lower than those of the control group 10 days and 20 days after treatment(P<0.05). After treatment, the peak blood flow velocity(Vp) and mean blood flow velocity(Vm) levels of anterior cerebral artery(ACA), middle cerebral artery(MCA) and posterior cerebral artery(PCA) showed an upward trend in the two groups. After treatments of 10 days and 20 days, the Vp and Vm levels of ACA, MCA and PCA in the study group were significantly higher than those in the control group(P<0.05). After treatment, the ADL scores, maximum grip strength and the pressure between the fingers showed an upward trend in the two groups. After treatments of 10 days and 20 days, the ADL scores, maximum grip strength and the pressure between the fingers of the study group were significantly higher than those of the control group(P<0.05). The total effective rate was 92.50% in the study group, and 75.00% in the control group, and the difference was statistically significant between the two groups(P<0.05). Conclusion　Butylphthalide injection combined with intelligent robot hand has a significant clinical effect on the acute cerebral infarction patients with hemiplegic hands. The combined therapy can effectively improve the patients′ nerve defects and cerebral blood perfusion, and help to improve the patients′ ADL.]]> 2021/7/5 12:12:34 CHEN Bin, WU Xian-ru, LING Zheng, et al. 615true ［Abstract］　Objective　To investigate the clinicopathological features of renal injury in plasma cell myeloma(PCM) presenting with tubulointerstitial nephritis. Methods　A case of PCM presenting with tubulointerstitial inflammation was reported. The clinical manifestations, histomorphological and electron microscopic features of the case were analyzed and the related literatures were reviewed. Results　A 34-year-old male patient was admitted to the hospital with acute renal insufficiency. Renal biopsy showed tubulointerstitial nephritis. Immunopathology showed the expression of kappa light chain. Electron microscopy showed sediment-like deposits on both sides of glomerulus and renal tubular basement membrane. Bone marrow biopsy confirmed the diagnosis of PCM. Conclusion　PCM can cause multiple organ damage. When the disease starts with renal insufficiency, it should be comprehensively evaluated by light microscopy, immunopathology and electron microscopy to avoid missed diagnosis, misdiagnosis and mistreatment.]]> 2021/7/5 12:12:34 LIU Meng-meng, WANG Xi-feng, LIU Xing-hua, et al. 614true ［Abstract］　Objective　To investigate the application value of percutaneous choledochoscopy in removal of severe acute pancreatitis(SAP) secondary to infected pancreatic necrosis(IPN). Methods　Twelve patients receiving removal of IPN tissues by using ultrasound or computed tomography(CT) guided percutaneous catheter drainage(PCD) and sinus dilation step by step under electronic choledochoscope in the Department of Hepatobiliary Surgery, the Fourth Affiliated Hospital of Guangxi Medical University from January 2016 to March 2019 were collected(the observation group), and their data were retrospectively analyzed. Meanwhile, 10 patients receiving laparotomy for removal of IPN were taken as the control group. The data were compared between the two groups. Results　The effective clearance rate of IPN was 100.00% in both groups. The incidence of pancreatic fistula in the observation group was 25.00%, and that in the control group was 20.00%, and the difference was not statistically significant(P>0.05). The bleeding rate of the observation group was 16.67%, and that of the control group was 0.00%, and the difference was no statistically significant(P>0.05). There was no intestinal fistula in the two groups. There was no postoperative residual pus cavity in the observation group. Four cases in the control group had some residual pus cavities after the second operation, with a residual pus cavity rate of 40.00%. There was statistically significant difference in the residual pus cavity rate between the two groups(P<0.05).The hospitalization expenses of the observation group were significantly less than those of the control group(P<0.05), and the hospitalization time of the observation group was significantly shorter than that of the control group(P<0.05). Among the patients in both groups, the longest follow-up was 2 years, but there was no recurrence. Conclusion　Debridement under percutaneous choledochoscope to treat SAP complicated with IPN has the advantages of small trauma, quick recovery, easy to operate on the patient repeatedly, low costs, short hospital stay and a few complications, which is worthy of clinical application.]]> 2021/7/5 12:12:34 JIANG Han-cheng, JIANG Shui-ming, ZHOU Min, et al. 613true ［Abstract］　Objective　To explore the correlation between the results of musculoskeletal ultrasound and the Traditional Chinese Medicine(TCM) syndrome of knee osteoarthritis(KOA). Methods　One hundred KOA patients(128 knees) who were treated in Xianhu Branch, the First Affiliated Hospital of Guangxi University of Chinese Medicine from January 2019 to January 2020 were selected, and were divided into the wind cold dampness group(17 cases, 24 knees), the damp and hot accumulated knot group(30 cases, 34 knees), the deficiency of liver and kidney group(34 cases, 40 knees) and the qi stagnation and blood stasis group(19 cases, 30 knees) according to the principles of Traditional Chinese Medicine(TCM) syndrome differentiation treatment. The knee joint synovial thickness, synovial blood flow, osteophytes, articular cartilage and subchondral bone destruction, joint effusion and popliteal cysts were detected by using musculoskeletal ultrasound technology, and the results of musculoskeletal ultrasound were compared among the four groups. Results　The positive rates of synovial thickening and synovial blood flow in the damp and hot accumulated knot group were significantly higher than those in the wind cold dampness group, the qi stagnation and blood stasis group and the deficiency of liver and kidney group(P<0.05).The positive rate of cartilage and subchondral bone destruction in the the deficiency of liver and kidney group was significantly higher than that in the wind cold dampness group, the qi stagnation and blood stasis group, and the damp and hot accumulated knot group(P<0.05). The positive rates of joint effusion in the damp and hot accumulated knot group and the qi stagnation and blood stasis group were significantly higher than those in the wind cold dampness group and the deficiency of liver and kidney group(P<0.05). The positive rate of popliteal cysts in the qi stagnation and blood stasis group was significantly higher than that in the wind cold dampness group and the deficiency of liver and kidney group(P<0.05). There was no significant difference in the positive rate of osteophyte formation among the four groups(P>0.05). Conclusion　There is a correlation between the results of knee musculoskeletal ultrasound and TCM syndrome types in patients with KOA, which provides an objective basis for TCM syndrome differentiation in patients with KOA.]]> 2021/6/3 21:44:38 DENG Wei, ZENG Ping, FENG Cheng-qin, et al. 612true ［Abstract］　Objective　To investigate the plasma lipoprotein-associated phospholipase A₂(Lp-PLA₂) level in overweight and obese patients with type 2 diabetes mellitus(T2DM), and its correlation with carotid intima-media thickness(IMT). Methods　Ninety-five T2DM patients who were admitted to the Affiliated Hospital of Guilin Medical University from January 2018 to August 2018 were selected. They were divided into simple T2DM group(BMI<24 kg/m², n=56) and overweight and obese T2DM group(BMI≥24 kg/m², n=39) according to their different values of Body Mass Index(BMI). The levels of fasting blood-glucose(FBG), total cholesterol(TC), triglyceride(TG), high density lipoprotein-cholesterol(HDL-C), low density lipoprotein-cholesterol(LDL-C), fasting insulin(FINS), insulin resistance index(IRI), glycated haemoglobin A1c(HbA1c), carotid IMT and Lp-PLA₂ were compared between the two groups. The correlation between Lp-PLA₂ and carotid IMT was analyzed. Results　The levels of FINS, IRI, TC, LDL-C, Lp-PLA₂ and carotid IMT in the overweight and obese T2DM group were significantly higher than those in the simple T2DM group, while the level of HDL-C in the overweight and obese T2DM group was significantly lower than that in the simple T2DM group(P<0.05). There were no statistically significant differences between the two groups in the levels of FBG, HbA1c and TG(P>0.05). There was a positive correlation between plasma Lp-PLA₂ level and carotid IMT level in the T2DM patients(r=0.388, P=0.000). There were positive correlations between plasma Lp-PLA₂ level and carotid IMT level in the simple T2DM group and the overweight and obese T2DM group(r=0.292, P=0.029; r=0.470, P=0.003). The results of multiple linear regression analysis showed that higher levels of BMI, LDL-C and Lp-PLA₂ were the main risk factors affecting carotid IMT thickening(P<0.05). Conclusion　Overweight and obese T2DM patients have higher levels of plasma Lp-PLA₂, and the level of Lp-PLA₂ is positively correlated with carotid IMT. The detection of this index may be beneficial to clinicians for early detection and intervention of diabetic macrovascular disease.]]> 2021/6/3 21:44:38 XUN Jing-qiong, KANG Sheng, TANG Min-juan, et al. 611true ［Abstract］　Objective　To investigate the effect of Sonic Hedgehog signaling pathway on the proliferation of mouse hemangioma endothelial cells(EOMA cells). Methods　EOMA cells were cultured regularly. Methyl thiazolyl tetrazolium(MTT) colorimetry was used to detect the effect of GANT61, a Sonic Hedgehog signaling pathway inhibitor, on the proliferation of EOMA cells. EdU staining and flow cytometry were used to detect the effects of GANT61 and Sonic Hedgehog signaling pathway agonist SAG on the proliferation of EOMA cells. The effects of GANT61 and SAG on the mRNA expressions of proliferating cell nuclear antigen(PCNA) and Cyclin D1 in EOMA cells were detected by real-time quantitative polymerase chain reaction(RT-qPCR). Results　After EOMA cells were treated with GANT61, the proliferation of EOMA cells was inhibited in a dose-and time-dependent manner, and the 50% inhibitory concentration(IC₅₀) value was 79.42 μmol/L at 24 h. The detection results of EdU staining and flow cytometry showed that GANT61 intervention inhibited the proliferation of EOMA cells, and SAG intervention promoted the proliferation of EOMA cells. The detection results of RT-qPCR showed that GANT61 intervention inhibited the expressions of PCNA and Cyclin D1 mRNA, and SAG intervention promoted the expressions of PCNA and Cyclin D1 mRNA. Conclusion　Sonic Hedgehog signaling pathway can regulate the proliferation of EOMA cells, which may be involved in the occurrence and development of hemangioma.]]> 2021/6/3 21:44:38 ZHANG Min-hong, ZENG Yong, LU Hong-fei 610true ［Abstract］　Objective　To explore the interventional effect of paeonol on the expressions of retinal stromal cell derived factor-1(SDF-1) and vascular endothelial growth factor(VEGF) of diabetic rats. Methods　Thirty-six male Sprague Dawley(SD) rats were randomly divided into normal control group(CON group), paeonol group(DPF group) and diabetes mellitus group(DM group), with 12 rats in each group. According to the length of intervention, each group was further divided into 1-month group, 3-month group and 5-month group, with 4 rats in each group. The diabetic rat model was established by intraperitoneal injection of streptozotocin. Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR) was used to detect the mRNA expression levels of SDF-1 and VEGF in each group. Hematoxylin-eosin(HE) staining was used to observe the number of endothelial cell nuclei breaking through the inner limiting membrane. Results　The detection results of RT-qPCR showed that the expression levels of SDF-1 mRNA and VEGF mRNA in the DM group were significantly higher than those in the CON group and the DPF group at the time points of 1-month, 3-month and 5-month after intervention(P<0.05), and the expression levels of DPF group were slightly higher than those of the CON group, but the differences were not statistically significant(P>0.05). The results of HE staining showed that the number of nuclei breaking through the inner limiting membrane of the retina in the DM and DPF groups was significantly increased compared with that in the CON group(P<0.05). The number of nuclei breaking through the inner limiting membrane of the retina in the DM group and the DPF group increased with the extension of the intervention time, and the number of the nuclei in the DPF group at each time point was significantly less than that in the DM group(P<0.05). Conclusion　Paeonol can inhibit retinal neovascularization by down-regulating the expressions of SDF-1 and VEGF in the retinas of diabetic rats.]]> 2021/6/3 21:44:38 ZHOU Qian-qian, TANG Ying 609true ［Abstract］　Objective　To evaluate the pelvic floor injury in primiparas with different delivery modes by Pelvic Organ Prolapse Quantification(POP-Q) scoring system. Methods　The primiparas giving birth in the Department of Obstetrics and Gynecology, the People′s Hospital of Guangxi Zhuang Autonomous Region, from November 2016 to December 2019 were selected and divided into cesarean section group(observation group) and vaginal delivery group(control group) according to different delivery modes, with 120 cases in each group. Pelvic floor function test and POP-Q score were performed in the two groups 6 weeks after delivery, and pelvic floor electric diagnosis and the incidence of stress urinary incontinence(SUI) were compared between the two groups. Results　The POP-Q scores of the observation group were better than those of the control group in the following aspects: the middle line of the anterior wall of the vagina 3cm away from the edge of the hymen(Aa), the farthest distance of the anterior wall of the vagina from the edge of the hymen(Ba), the middle line of the posterior wall of the vagina 3cm away from the edge of the hymen(Ap), the farthest distance of the posterior wall of the vagina from the hymen(Bp), the length of the genital hiatus(GH), the length of the perineal body(PB), and the total vaginal length(TVL), with statistically significant differences between the two groups(P<0.05). However, there were no significant differences in the D-point level between the two groups(P>0.05). The prolapse grades of anterior vaginal wall and posterior vaginal wall in the observation group were lower than those in the control group(P<0.05). There was no significant difference in the prolapse grade of the uterus between the two groups(P>0.05). There were no significant differences in the incidence of postpartum SUI, abnormal vaginal pressure, abnormal muscle fatigue degree and abnormal pelvic floor muscle strength between the two groups(P>0.05). Conclusion　In the immediate postpartum period, there are no significant differences in the uterine prolapse rate and grading, and the incidence of SUI between two groups of primiparas adopting the two modes of delivery respectively. Combined with POP-Q score, cesarean section has less impact on the postpartum pelvic floor function.]]> 2021/6/3 21:44:38 ZHANG Ji-hong, LIANG Xu-xia, ZHANG Chun 608true ［Abstract］　Objective　To analyze the effect of retrograde squeeze to remove stones combined with lacrimal tube implantation on chronic lacrimal canaliculitis. Methods　A retrospective analysis was performed on 35 patients(35 eyes) with chronic lacrimal canaliculitis who were treated at Shanghai Aier Eye Hospital from June 2018 to December 2020, including 10 cases of upper canaliculitis and 25 cases of lower canaliculitis. None of them was complicated with chronic dacryocystitis. The clinical symptoms and signs of all the patients were recorded. All the patients underwent retrograde squeeze to remove stones combined with lacrimal tube implantation under local anesthesia. Antibiotic eye drops were continuously given to the patients to flush the lacrimal duct and conjunctival sac for three days after operation. The silicone tube was removed one month after surgery. One week, one month and three months after operation, the improvement of pus in the operated eyes, the morphology of lacrimal canaliculi and the results of lacrimal irrigation were recorded in each time period. Results　After treatment, 35 patients were discharged without complications. The symptoms of epiphora and pyorrhea disappeared in the 35 patients 3 months after operation. The morphological observation of lacrimal canaliculi showed that 30 eyes were normal, and 3 eyes were dilated and hypertrophic, and 2 eyes were incomplete obstruction. The results of lacrimal irrigation showed that 30 eyes were passable, and 5 eyes were not completely passable. The clinical cure rate was 85.71%. Conclusion　Retrograde squeeze to remove stones combined with lacrimal tube implantation is effective in treatment of chronic canaliculitis. It can effectively solve the symptoms of pyorrhea and has little damage to the lacrimal passage. However, it is necessary to master the indications before operation, to remove the stones as many as possible during the operation, to avoid damaging the mucosa of lacrimal canaliculi during the intubation, and to strengthen the application of sensitive antibiotics for lacrimal duct and conjunctival sac postoperatively.]]> 2021/6/3 21:44:38 PAN Shu-ling, CHEN Yan 607true ［Abstract］　Objective　To compare the quality of bowel preparation at different time of bowel preparation for colonoscopy. Methods　A total of 130 non-anesthetic outpatients and inpatients who underwent colonoscopy in Zhangzhou Hospital Affiliated to Fujian Medical University from October 2019 to January 2020 were divided into observation group(64 cases) and control group(66 cases) by random number table method. The observation group was given colon cleanser orally from 5:00 a.m. to 7:00 a.m. and colonoscopy was completed in the morning. The control group was given colon cleanser orally from 10:00 a.m. to 12:00 a.m. and colonoscopy was completed in the afternoon. Boston Bowel Preparation Scale(BBPS) score and Intestinal Cavity Bubble score were performed by the same experienced endoscopist after the examination, and the occurrence of adverse reactions in the patients during bowel preparation was recorded. Results　Bowel preparation and total colonoscopy were completed in all the 130 patients. The total scores of BBPS, the BBPS scores of the left hemicolon, the BBPS scores of the middle colon and the BBPS scores of the right hemicolon in the observation group were significantly higher than those in the control group(P<0.01). The scores of Intestinal Cavity Bubble in the observation group were significantly lower than those in the control group(P<0.05). The incidence of abdominal distension in the observation group was significantly lower than that in the control group(P<0.05). There were no significant differences in the other adverse reactions between the two groups(P>0.05). Conclusion　Patients taking colon cleanser orally from 5:00 a.m. to 7:00 a.m. in the morning have better bowel preparation quality and less adverse reaction of abdominal distension, which will be more conducive to the detection of intestinal microscopic lesions and the successful completion of colonoscopy.]]> 2021/6/3 21:44:38 HUANG Cai-yun, ZHAO Mei-yan, LIN Shu-hui, et al. 606true ［Abstract］　Objective　To observe the effect of Shenfu injection on cardiac diastolic function in patients with septic shock and its therapeutic effect. Methods　Sixty patients with septic shock were randomly divided into observation group and control group according to the random number table method, with 30 cases in each group. Both groups were given standard conventional cluster therapy(fluid resuscitation, anti-infection, oxygen therapy, etc.). On the basis of this conventional treatment, the observation group was given Shenfu injection 100 ml added with 5% glucose 100 ml for intravenous drip, once a day. The serum levels of N-terminal pro-brain natriuretic peptide(NT-proBNP) and cardiac troponin T(cTnT) were detected before and 7 days after treatment in the two groups. Left ventricular end-diastolic diameter(LVEDD), left ventricular end-diastolic volume(LVEDV), peak velocity of early mitral valve dilation(E) and early diastolic velocity of mitral annulus(E′) were measured by echocardiography before and 7 days after treatment, and E/E′ was calculated. At the same time, Acute Physiology and Chronic Health Evaluation Ⅱ(APACHEⅡ) score and Sequential Organ Failure Assessment(SOFA) score were recorded on the first day of admission and on the seventh day of treatment. The duration of mechanical ventilation and the length of Intensive Care Unit(ICU) stay were recorded. Results　After 1 week of treatment, the levels of NT-proBNP, cTnT, LVEDD, LVEDV and E/E′ in the observation group were significantly lower than those in the control group(P<0.05). The APACHEⅡ scores and the SOFA scores in the observation group were significantly lower than those in the control group after treatment(P<0.05). The duration of mechanical ventilation and the length of ICU stay in the observation group were significantly shorter than those in the control group(P<0.05). Conclusion　Shenfu injection can improve the cardiac diastolic function of septic shock patients, reduce the severity of multiple organ dysfunction syndrome(MODS) and improve the therapeutic effect.]]> 2021/6/3 21:44:38 SHI Yan-jie, WANG Er-ling, LIU Xiao-gang, et al. 605true ［Abstract］　Objective　To analyze the hospital infection and community infection of Escherichia coli in the Department of General Surgery of Jintan District People′s Hospital Affiliated to Jiangsu University and to explore its drug resistance, so as to provide theoretical basis for rational drug use in clinical practice. Methods　The Escherichia coli isolated from the clinical specimens of the inpatients in the Department of General Surgery of Jintan District People′s Hospital Affiliated to Jiangsu University from January 2017 to December 2019 was selected as the research object, which was divided into the hospital infection group and the community infection group. The incidence of hospital infection, specimen distribution and multi-drug resistance of Escherichia coli were analyzed, and the resistance rates of Escherichia coli to common antibiotics were compared between the two groups. Results　Thirty-two strains(accounting for 17.78%) of Escherichia coli causing hospital infection in the Department of General Surgery and 148 strains(accounting for 82.22%) causing community infection were detected. The incidence of hospital infection of Escherichia coli increased with the increase of years(P<0.05), and the Escherichia coli was mainly detected from wound drainage fluid. Compared with community infected Escherichia coli, hospital infected Escherichia coli was more likely to produce multiple drug resistance(P<0.05), and the hospital infected Escherichia coli had higher drug resistance rates to carbapenems, cephalosporins and fluoroquinolones(P<0.05). Extended-spectrum β-lactamases(ESBLs)-producing Escherichia coli had higher drug resistance rates to compound sulfamethoxazole, cephalosporins and fluoroquinolones than non-ESBLs-producing Escherichia coli(P<0.05). The resistance rates of ESBLs-producing Escherichia coli to imipenem and tobramycin in the hospital infection group were higher than those in the community infection group(P<0.05). Conclusion　Both hospital infected Escherichia coli and community infected Escherichia coli are sensitive to cefoxitin and amikacin. Carbapenems, cefoxitin and amikacin can be used as the first choice for the treatment of ESBLs-producing Escherichia coli infection. The prevention and control measures of multi-drug resistant bacteria should be actively implemented, and antibacterial drugs should be selected reasonably according to the patients′ condition, drug sensitivity and economic conditions, so as to prevent the spread of Escherichia coli in hospitals.]]> 2021/6/3 21:44:38 HUANG Cong, WANG Yu-mu 604true ［Abstract］　Objective　To investigate the imaging manifestations and pathological features of hepatic epithelioid angiomyolipoma(HEAML). Methods　The clinical data of 15 HEAML patients confirmed by surgery and pathology in Sichuan Cancer Hospital from September 2012 to September 2019 were selected, and their imaging and pathological characteristics were summarized. Results　A clear boundary lesion and slightly lower density/signal were showed in the 15 cases of HEAML receiving plain scans. A small amount of fat composition was observed in the lesions of 5 cases. In the arterial phase of the enhanced scan, obvious inhomogeneous contrast was showed in the 15 cases, including enriched and enhanced blood vessels, patchy or nodular-like enhancement. In the portal vein phase and delayed phase, 11 lesions had a continuous enhancement with the characteristics of “fast-in and slow-out” enhancement mode relative to the surrounding liver parenchyma, 4 lesions with “fast-in and fast-out” enhancement mode, 4 lesions with deformed drainage veins, and 1 lesion with pseudocapsules. Epithelial tumor components, fat-like cells of different proportions, and thick-walled blood vessels could be seen under the microscope, and the immunohistochemistry features of all the 15 lesions showed positive melanin markers human melanoma black(HMB45) and melanoma-antigen(Melan-A), and smooth muscle marker smooth muscle actin(SMA), and negative creatine kinase(CK), S-100, hepatocyte(Hep Par 1), and alpha-fetoprotein(AFP). Conclusion　HEAML has some characteristic imaging manifestations. The characteristics of enriched and enhanced blood vessels and deformed drainage veins in the tumor are helpful for diagnosis, while the confirmed diagnosis still highly depends on pathological examination.]]> 2021/5/8 18:36:31 LIANG Xu, YAN Jia-xin, DENG He-ping, et al. 603true ［Abstract］　Objective　To analyze the risk factors of portal vein thrombosis(PVT) in patients with liver cirrhosis, and to provide clinical basis for early diagnosis and treatment of PVT. Methods　The data of clinical medical records of 170 liver cirrhosis patients admitted to the Department of Gastroenterology, the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2018 to December 2020 were collected. Among them, 34 patients with PVT were taken as the PVT group, and the other 136 patients without PVT as the control group. The differences in demographic data, etiology, laboratory examination, imaging examination and other aspects were compared between the two groups. Multivariate logistic regression model was used to explore the risk factors of PVT in the liver cirrhosis patients. Results　The proportion of width of main portal vein, prothrombin time(PT), splenectomy, spontaneous peritonitis, and Child-Pugh class C in the PVT group was greater than that in the control group, and the difference was statistically significant(P<0.05). The results of multivariate logistic regression analysis showed that prolonged PT and complicated spontaneous peritonitis were the risk factors of PVT in liver cirrhosis. Conclusion　Prolonged PT and complicated spontaneous peritonitis are the risk factors of PVT in patients with liver cirrhosis, and clinicians should pay attention to screening and follow-up for the patients.]]> 2021/5/8 18:36:31 ZHOU Qing-nan, ZHANG Guo, NONG Bing, et al. 602true ［Abstract］　Objective　To analyze the correlation between the levels of sex hormones and body fat distribution in middle-aged male patients with diabetes mellitus type 2 and different somatotypes. Methods　Fifty middle-aged male patients with diabetes mellitus type 2 admitted to the Department of Endocrinology and Metabolism, the People′s Hospital of Guangxi Zhuang Autonomous Region from May 2018 to November 2019 were selected and divided into the normal weight group(group A, BMI=18.5-23.9 kg/m², n=14), the overweight group(group B, 24.0-27.9 kg/m², n=20) and the obesity group(group C, ≥28.0 kg/m², n=16) according to different levels of Body Mass Index(BMI). The somatotypes, body fat distribution, blood fat and sex hormone levels were compared among the three groups, and the correlation between sex hormone levels and body fat distribution was analyzed. Results　There was statistically significant difference in waist circumference among the three groups(P<0.05), in which group C had significantly larger waist circumference than group A and group B(P<0.05), and group B had significantly larger waist circumference than group A(P<0.05). There was statistically significant difference in the level of triglyceride(TG) among the three groups(P<0.05), in which group A had significantly lower TG level than group B and group C(P<0.05). There was statistically significant difference in the level of total testosterone(TT) among the three groups(P<0.05), in which group C had significantly lower TT level than group A(P<0.05). There were statistically significant differences in the proportion of body fat, the proportion of fat in the “Android area” and the A/G value(P<0.05) among the three groups(P<0.05), in which group C had significantly higher proportion of body fat and higher proportion of fat in the “Android area” than group A and group B(P<0.05), and group C had significantly higher A/G value than group A(P<0.05). The results of Pearson correlation analysis showed that TT was negatively correlated with waist circumference, luteinizing hormone(LH), the proportion of fat in the “Android area” and the A/G value(P<0.05). Conclusion　TT level in middle-aged male patients with diabetes mellitus type 2 is closely related to body fat distribution. Visceral fat accumulation is an important risk factor for the decrease of TT level.]]> 2021/5/8 18:36:31 HUANG Jian-hao, LU Jie, YU Yan-qing, et al. 601true ［Abstract］　Objective　To observe the clinical efficacy of suspended core muscle group training in treatment of lumbar intervertebral disc herniation, and its effects on the integral values of electromyogram(iEMGs) of erector spine muscles and multifidus muscles. Methods　Sixty-four patients with lumbar intervertebral disc herniation admitted to the Department of Rehabilitation Medicine, Jilin Province FAW General Hospital were selected from January 2018 to November 2020, and they were divided into observation group and control group by random number table method, with 32 cases in each group. The control group received conventional rehabilitation treatment, and the observation group received the same treatment as the control group plus suspended core muscle group training. The iEMG of erector spine muscles, the iEMG of multifidus muscles, the Visual Analogue Scale(VAS) scores and the Japanese Orthopaedic Association(JOA) low back pain scores were compared between the two groups before treatment and 4 weeks after treatment. Results　Before treatment, there were no significant differences in the iEMGs of erector spine muscles and multifidus muscles on the affected side and the healthy side between the two groups(P>0.05). After 4 weeks of treatment, the iEMGs of erector spine muscles and multifidus muscles on the affected side of the observation group were significantly higher than those of the control group(P<0.05), and there were no significant differences compared with those on the healthy side of the same group(P>0.05). Compared with those before treatment, the VAS scores significantly decreased in the two groups after 4 weeks of treatment, and the JOA lower back pain scores increased significantly(P<0.05). The VAS scores of the observation group were significantly lower than those of the control group, while the JOA low back pain scores of the observation group were significantly higher than those of the control group(P<0.05). Conclusion　Suspended core muscle group training is beneficial to the recovery of muscle strength in patients with lumbar intervertebral disc herniation, and the treatment effect is remarkable.]]> 2021/5/8 18:36:31 LI De-quan, LI Jia-hang, JIA Dan, et al. 600true ［Abstract］　Objective　To analyze the value of ultrasound combined with molybdenum target in early invasive breast cancer patients with tumor diameter≤2 cm to avoid sentinel lymph node biopsy(SLNB). Methods　A retrospective study was conducted on 122 early invasive breast cancer patients with tumor diameter≤2 cm and SLNB was performed on them during the operation in the First Affiliated Hospital of Chongqing Medical University from January 2018 to June 2019. The results of ultrasound, molybdenum target, MRI and SLNB were collected and compared with the results of postoperative axillary lymph node biopsy(gold standard) respectively. Results　Of the 122 patients, 29 patients showed positive results of postoperative axillary lymph node biopsy. In the Kappa consistency test of SLNB, ultrasound, MRI and molybdenum target with gold standard, the Kappa values were 0.721, 0.668, 0.620, 0.422, respectively, and the difference was statistically significant(P<0.05). In the Kappa test of ultrasound, molybdenum target and MRI in the combination of pairwise or triple diagnosis with gold standard, the Kappa value of ultrasound combined with molybdenum target was the highest(Kappa=0.702, P<0.05). Conclusion　The result of ultrasound combined with molybdenum target in the diagnosis of axillary lymph node metastasis is relatively highly consistent with that of the gold standard, which provides strong evidence support for the early invasive breast cancer patients with tumor diameter≤2 cm to avoid SLNB.]]> 2021/5/8 18:36:31 CHEN Huan, YANG Guang-lun, YANG Luo, et al. 599true ［Abstract］　Objective　To study the development of visual function in children with spontaneous regression of retinopathy of prematurity(ROP). Methods　A total of 238 children with spontaneous regression of ROP(476 eyes) were retrospectively collected as the observation group. According to the ages of the patients at the time of examination, they were divided into 3-≤4 years old group(n=72, 144 eyes), 4-≤5 years old group(n=65, 130 eyes), and 5-≤6 years old group(n=101, 202 eyes). A total of 262 children(524 eyes) without ROP in the same period were taken as the control group, including 89 cases(178 eyes) of the 3-≤4 years old group, 82 cases(164 eyes) of the 4-≤5 years old group, and 91 cases(182 eyes) of the 5-≤6 years old group. The refractive status, biological parameters and macula central foveal thickness of the children′ eyes at different ages were measured and compared between the two groups. Results　There were no statistically significant differences in the refractive status, biological parameters(except axial length and anterior chamber depth) and macula central foveal thickness of the eyes between the two groups(P>0.05). Among the different age groups, there were statistically significant differences in the diopter of spherical power, axial length, anterior chamber depth, lens thickness and macular foveal retinal thickness(P<0.05). Conclusion　Compared with the children without ROP, the children with spontaneously regressed ROP have no significant differences in the refractive status, biological parameters and macula central foveal thickness of the eyes from age 3 to 6. They are mainly emmetropia and hyperopia, and the development of eyeballs is not significantly affected. Attention should be paid to the existence of visual dysfunction in children with spontaneous regression of ROP, and the monitoring and treatment of visual function should be strengthened during their growth process to protect the development of visual function to the greatest extent.]]> 2021/5/8 18:36:31 SUN Guang-hua, MAN Teng-teng, ZHANG Nan, et al. 598true ［Abstract］　Objective　To explore the value of preoperative contrast-enhanced ultrasound and intraoperative fast frozen pathological sections in the diagnosis of vessels encapsulated tumor clusters(VETC) carcinoma nest-type hepatocellular carcinoma(HCC) and the selection of the optimal surgical method. Methods　The clinical data of 164 HCC patients confirmed by postoperative routine pathology from October 2013 to October 2018 were collected. Immunohistochemistry(IHC) staining for CD34 was used to observe the morphologic features of VETC carcinoma nest-type/non-nest-type HCC. The proportion of paracancerous metastases in VETC carcinoma nest-type and VETC carcinoma non-nest-type HCC was calculated. The patients were followed up and the recurrence rates of VETC carcinoma nest-type HCC patients and VETC carcinoma non-nest-type HCC patients were investigated 2 years after surgery. The effects of anatomic hepatectomy and non-anatomic hepatectomy on the recurrence rate of VETC carcinoma nest-type HCC patients 2 years after operation were analyzed. The value of preoperative contrast-enhanced ultrasound and intraoperative fast frozen pathological sections in the diagnosis of VETC carcinoma nest-type HCC was analyzed. Results　Among the 164 cases of HCC, 40.24%(66/164) of them were VETC carcinoma nest-type HCC, and the incidence of paracancerous metastases in the VETC carcinoma nest-type HCC patients［30.30%(20/66)］ was significantly higher than that in the VETC carcinoma non-nest-type HCC patients［10.20%(10/98)］(P<0.05). The recurrence rates of VETC carcinoma nest-type HCC and VETC carcinoma non-nest-type HCC 2 years after operation were 53.33% and 36.90%, respectively. In the VETC carcinoma nest-type HCC patients, anatomic hepatectomy could significantly reduce the recurrence rate of HCC patients 2 years after operation(P<0.05). The sensitivity, specificity and accuracy of preoperative contrast-enhanced ultrasound in the diagnosis of VETC carcinoma nest-type HCC were 81.82%, 91.84% and 87.80%, respectively, which was in good agreement with the postoperative routine pathological results(Kappa=0.744, P=0.000). The sensitivity, specificity and accuracy of intraoperative fast frozen pathological sections in the diagnosis of VETC carcinoma nest-type HCC were 93.94%, 91.84% and 92.68%, which was in good agreement with the postoperative routine pathological results(Kappa=0.849, P=0.000). Conclusion　Preoperative contrast-enhanced ultrasound and intraoperative fast frozen pathological sections can effectively diagnose VETC carcinoma nest-type HCC, and anatomical hepatectomy is the preferred surgical method for patients with VETC carcinoma nest-type HCC.]]> 2021/4/2 14:58:35 YANG Jian-rong, ZHONG Jing-tao, LIU Tian-qi, et al. 597true ［Abstract］　Objective　To analyze the expressions of docking protein 3(DOK3) and protein tyrosine kinase 7(PTK7) in different grades of colon adenoma tissues, and to explore their roles in the occurrence and development of colon cancer. Methods　The specimens from 22 cases of colon adenoma with low-grade dysplasia(LGD), 23 cases of colon adenoma with high-grade dysplasia(HGD), 22 cases of colon cancer and 20 cases of non-tumor mucosal tissue adjacent to the tumor who were diagnosed by the Department of Pathology, Nanxishan Hospital of Guangxi Zhuang Autonomous Region were collected during January 2015 and December 2018. Immunohistochemical staining method was used to detect the expressions of DOK3 and PTK7 in different types of specimens. Results　Both DOK3 and PTK7 were mainly expressed in the cytoplasm and membrane of glandular epithelium. The high expression rates of DOK3 in non-tumor mucosal tissues adjacent to the tumor and LGD tissues were significantly higher than those in HGD tissues and colon cancer tissues(P<0.05). The high expression rate of DOK3 in HGD tissues was also significantly higher than that in colon cancer tissues(P<0.05). The high expression rates of PTK7 in colon cancer tissues and HGD tissues were significantly higher than those in non-tumor mucosal tissues adjacent to the tumor and LGD tissues(P<0.05). The high expression rate of PTK7 in colon cancer tissues was also significantly higher than that in HGD tissues(P<0.05). Conclusion　DOK3 may act as a tumor suppressor, and PTK7 may act as a cancer promoter involving in the occurrence and development of colon cancer.]]> 2021/4/2 14:58:35 MENG Yun-chao, ZHANG Qi-fang, PENG Jia-yin, et al. 596true ［Abstract］　Objective　To analyze the clinical efficacy of laparoscopic D2 radical resection combined with complete mesocolic excision(CME) in treatment of advanced gastric cancer. Methods　The clinical data of 80 patients with gastric cancer who underwent therapeutic laparoscopy in the Second Affiliated Hospital of Zhengzhou University from January 2018 to December 2018 were retrospectively analyzed. The patients were divided into the study group(receiving laparoscopic D2 radical resection+CME treatment) and the control group(treated with standard D2 radical resection under laparoscopic surgery) according to different surgical procedures, with 40 cases in each group. The operation time, intraoperative blood loss, number of lymph node dissection, postoperative functional recovery, incidence of postoperative complications and one-year survival after surgery were compared between the two groups. Results　Compared with the control group, the study group had shorter operation time, less intraoperative blood loss, and more lymph node dissections, and the differences were statistically significant between the two groups(P<0.05). The first exhaust time, the first feeding time and hospitalization time of the study group was significantly shorter than that of the control group(P<0.05), and the drainage volume of the abdominal cavity drainage tube of the study group was significantly less than that of the control group(P<0.05). The incidence of postoperative complications in the study group was significantly lower than that in the control group(22.50% vs 52.50%; χ²=10.912, P=0.000). During the follow-up, 8 cases(20.00%) in the study group had tumor recurrence, and 16 cases(40.00%) in the control group had tumor recurrence; 2 cases(5.00%) in the study group died, and 5 cases(12.50%) in the control group died. The results of log-rank test showed that there was no significant difference in postoperative survival between the two groups(χ²=2.735, P=0.143). Conclusion　Compared with standard D2 radical resection, D2 radical resection+CME surgical procedure has shorter operation time, less intraoperative blood loss, more lymph node dissections, faster postoperative intestinal function recovery, and less postoperative complications. D2 radical resection+CME surgical procedure is worthy of clinical recommendation.]]> 2021/4/2 14:58:35 GUO Fan, HUANG Lin-kai, ZHU Jia-jia, et al. 595true ［Abstract］　Objective　To compare the clinical effect of microwave ablation for recurrent hepatocellular carcinoma under the guidance of laparoscopic ultrasound versus percutaneous ultrasound. Methods　The clinical data of 90 patients with recurrent hepatocellular carcinoma in Eastern Hospital, Sichuan Provincial Medical Sciences Academy & Sichuan Provincial People′s Hospital from June 2015 to February 2018 were retrospectively analyzed. The patients were divided into observation group and control group according to different ways of microwave ablation, with 45 cases in each group. The observation group received laparoscopic ultrasound-guided microwave ablation, and the control group received percutaneous ultrasound-guided microwave ablation. The intraoperative blood loss, operative time, postoperative hospital stay, total hospital stay, lesion diameter and ablation time, cumulative survival rate and recurrence-free survival rate were compared between the two groups. Results　The intraoperative blood loss in the control group was significantly less than that in the observation group(P<0.05). The operative time, ablation time, the postoperative hospital stay and total hospital stay in the control group was significantly shorter than that in the observation group(P<0.05). 80.3%(49/61) of the lesions in the observation group and 3.4%(2/58) of the lesions in the control group were at specific sites. After 0.5, 1 and 1.5 years of follow-up, the cumulative survival rates of the observation group were 100.0%, 88.9% and 75.6% respectively, and those of the control group were 100.0%, 82.2% and 71.1% respectively, and there were no significant differences between the two groups(P>0.05); the recurrence-free survival rates of the observation group were 71.1%, 60.0% and 37.8% respectively, and those of the control group were 66.7%, 53.3% and 31.1% respectively, and there were no significant differences between the two groups(P>0.05). Conclusion　Percutaneous ultrasound-guided microwave ablation treatment is more minimally invasive, while laparoscopic ultrasound-guided microwave ablation is suitable for the lesions at specific sites. Percutaneous ultrasound-guided microwave ablation treatment is suitable for the patients who cannot be treated with laparotomy, which makes the scope of the treatment for recurrent hepatocellular carcinoma wider.]]> 2021/4/2 14:58:35 GAO Nan, MU Pei-yuan, BAI Yuan-yuan, et al. 594true ［Abstract］　Objective　To investigate the surgical techniques and safety of phacoemulsification for cataract patients with shallow anterior chamber. Methods　Forty-six cataract patients(46 eyes) with shallow anterior chamber who received treatment of phacoemulsification were selected in the Department of Ophthalmology of the Second People′s Hospital of Beihai City from January 2018 to January 2019. The patients′ preoperative and postoperative visual acuity, intraocular pressure, central anterior chamber depth(CACD), angle opening distance(AOD), and corneal endothelial cell density were compared. The incidence of postoperative complications was analyzed. Results　The preoperative mean visual acuity of the patients was (0.28±0.16), and their postoperative mean visual acuity improved to (0.62±0.22), with a significant difference(t=8.688, P=0.000). Compared with those before operation, the intraocular pressure was reduced and CACD was increased in the patients, and the differences were statistically significant(P<0.05). After treatment, the AOD500 values of the “3 o′clock”, “6 o′clock”, “9 o′clock” and “12 o′clock” positions of the operated eyes were significantly increased compared with those before operation(P<0.05). The intraoperative iris injury occurred in 3 cases(6.52%), and the partial rupture of suspensory ligament in 1 case(2.17%), but the intraocular lens were still implanted safely and successfully. The postoperative corneal edema occurred in 16 cases(34.78%) and the patients recovered one month after operation. No severe complications such as posterior capsule rupture and corneal decompensation occurred. Conclusion　Phacoemulsification can effectively improve the crowding of the anterior segment of cataract patients with shallow anterior chamber, and has good efficacy and safety. The key to the success of the operation is the preoperative evaluation and preparation, and the reasonable application of surgical techniques during the operation.]]> 2021/4/2 14:58:35 LI Jun-ning, LIN Chen-juan, HE Zhen 593true ［Abstract］　Objective　To analyze the prevalence and risk factors of chronic kidney disease in civil servants receiving health examination in Guangzhou City. Methods　The data of 36 762 civil servants(including some retired civil servants) who underwent health examination in Guangzhou Cadre Health Management Center from January 2019 to December 2019 were collected. According to the results of the health examination and laboratory examination, the detection rates of proteinuria, hematuria and chronic kidney disease were calculated, and the risk factors related to chronic kidney disease were analyzed. Results　The prevalence rates of proteinuria, hematuria and chronic kidney disease in the 36 762 civil servants receiving health examination were 2.25%, 3.26% and 7.04% respectively. Binary logistic regression analysis showed that advanced age(OR=3.042, 95%CI=2.647-3.496), female(OR=1.412, 95%CI=1.292-1.542), higher Body Mass Index(BMI)(OR=1.221,95%CI=1.079-1.381), hypertension(OR=1.883, 95%CI=1.712-2.070), diabetes(OR=1.578, 95%CI=1.409-1.766), hyperuricemia(OR=1.272, 95%CI=1.169-1.385), renal structural dysfunction(OR=1.769, 95%CI=1.617-1.936) were the risk factors of chronic kidney disease. Conclusion　The prevalence of chronic kidney disease is relatively high in the civil servants receiving health examination in Guangzhou City, and health examination is an important way to screen out chronic kidney disease. Control of blood glucose, blood pressure and blood uric acid may help to reduce the incidence of chronic kidney disease.]]> 2021/4/2 14:58:35 CHEN Xi, WANG Zong-quan, SHU Chen-hong, et al. 592true ［Abstract］　Objective　To explore the effect of phenethyl isothiocyanate(PEITC) combined with adriamycin(ADM) on the apoptosis of osteosarcoma cells. Methods　Osteosarcoma U2-OS cell line was selected and the cells were divided into PEITC group, ADM group, PEITC+ADM group and blank control group according to different treatment methods. Methyl thiazolyl tetrazolium(MTT) method was used to detect the drug effects of different treatment groups on the proliferation of U2-OS cells. Terminal deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL) method was used to determine the apoptosis of U2-OS cells in different treatment groups. Western blot was used to detect the expressions of Caspase-3, Fas and FasL proteins in U2-OS cells in different treatment groups. Results　50% inhibiting concentration(IC50) of PEITC and ADM on U2-OS cells were 4.37 μM and 6.61 μg/ml, respectively. Compared with PEITC or ADM treatment alone, the combined use of the two drugs had a higher proliferation inhibitory rate on U2-OS cells(P<0.05). Low-dose PEITC combined with ADM produced a synergistic effect, while high-dose PEITC combined with ADM produced an additive effect. Compared with single-drug treatment, PEITC combined with ADM could significantly increase the apoptosis rate of U2-OS cells(P<0.05), and increased the activity and expression of Caspase-3 protein(P<0.05). Conclusion　PEITC enhances the apoptosis of osteosarcoma cells induced by ADM, which may be related to the increased activity and up-regulation of Caspase-3 protein. This provides a reference for the clinical application of PEITC combined with ADM.]]> 2021/4/2 14:58:35 FAN Qie, LI Wen-hao, QIU De-zan, et al. 591true ［Abstract］　Objective　To observe the clinical efficacy of platelet-rich plasma combined with gabapentin in treatment of zoster-related neuralgia. Methods　Thirty-six patients with zoster-related neuralgia treated in the Department of Pain Management of the Second Affiliated Hospital of Guangxi Medical University from October 2019 to April 2020 were selected, and were divided into observation group and control group by random number table method, with 18 cases in each group. The control group was treated with routine antiviral therapy and oral gabapentin treatment. The observation group was treated with the same treatment as the control group plus ultrasound-guided injection of platelet-rich plasma into target nerve. The clinical efficacy and pain degree were compared between the two groups, and the occurrence of adverse reactions during the treatment of the two groups were recorded. Results　After treatment, the Numerical Rating Scale(NRS) scores in both groups showed a downward trend, and the differences were statistically significant compared with those before treatment in the same group(P<0.05). After treatment, the NRS scores of the observation group were significantly lower than those of the control group at each time point(P<0.05). In the observation group, 13 cases were clinically cured, 4 cases clinically improved, and 1 case ineffective; in the control group, 6 cases were clinically cured, 6 cases clinically improved, and 6 cases ineffective. The clinical effect of the observation group was better than that of the control group(Z=2.491, P=0.013). There were no serious adverse reactions during the treatment of the two groups. Conclusion　Compared with traditional drug therapy alone, ultrasound-guided platelet-rich plasma injection has the advantages of better analgesic effect and less adverse reactions in treatment of zoster-related neuralgia, which provides a new idea for the treatment of zoster-related neuralgia.]]> 2021/3/5 17:41:47 ZHOU Zeng-hua, LIAO Sha, HE Rui-lin, et al. 590true ［Abstract］　Objective　To observe the application effect of three-lumen gastrojejunal tube on treatment of upper digestive tract diseases. Methods　The clinical data of 21 patients with upper digestive tract diseases treated with three-lumen gastrojejunal tube in the People′s Hospital of Guangxi Zhuang Autonomous Region from March 2017 to December 2019 were retrospectively analyzed. The nasal feeding time, drainage volume and drainage events were recorded. The changes of electrolytes, nutritional indicators, and immune indicators before and after treatment were analyzed. Results　The 21 patients were smoothly discharged from the hospital after treatment without serious complications. In 1 case, the catheter was accidentally detached. In 3 cases, the catheters were blocked due to improper feeding. After treatment, the patients′ Body Mass Index(BMI), K⁺, Na⁺, Ca²⁺, lymphocyte count and prealbumin level showed an upward trend(P<0.05). Compared with those before treatment, the levels of total albumin and albumin did not change significantly after treatment(P>0.05). Conclusion　It is safe and feasible to perform gastrointestinal decompression and enteral nutrition in patients with upper digestive tract diseases by using three-lumen gastrojejunal tube.]]> 2021/3/5 17:41:47 WANG Long, MAI Wei, LI Lei, et al. 589true ［Abstract］　Objective　To study the effects of conditioned medium(CM) of human periodontal ligament stem cells(hPDLSCs) on cell morphology and proliferative characteristics of mouse embryonic osteoblasts(MC3T3-E1) cells. Methods　The hPDLSCs were isolated from the pericementum(periodontal ligament) tissues of the healthy premolars and the third molars, and were cultured. Immunofluorescence was used to detect the expressions of vimentin, pan cytokeratin(PCK) and the early mesenchymal stem cell marker STRO-1 in the obtained cells to identify the source of the cells. The CM of hPDLSCs(hPDLSCs-CM) were prepared for the intervention treatment of MC3T3-E1 cells, and the morphological characteristics and proliferation ability of MC3T3-E1 cells were compared between the intervention group and the control group(without treatment of hPDLSCs-CM). Results　Immunofluorescence staining showed that hPDLSCs showed the characteristics of vimentin and STRO-1 positive expressions, and PCK negative expression. The MC3T3-E1 cells in both groups showed the morphological characteristics of long spindles and polygons, and adherent growth. The results of methyl thiazolyl tetrazolium(MTT) experiment showed that the number and proliferation activity of MC3T3-E1 cells in the two groups showed an upward trend with the increase of culture time. On the 3rd, 5th and 7th day of culture, the OD value of the intervention group was significantly greater than that of the control group, and the difference was statistically significant(P<0.05). The results of cell cycle analysis showed that the proportion of cells in G₂/M+S phase in the intervention group and the control group were (31.43±0.59)% and (19.11±0.24)%, respectively, and the difference was statistically significant(t=43.393,P=0.000). Conclusion　Intervention with hPDLSCs-CM does not affect the morphological characteristics of MC3T3-E1 cells, and can enhance their proliferation ability.]]> 2021/3/5 17:41:47 LIU Yan, ZHAO Xiao-xia, WANG Jing-yu, et al. 588true ［Abstract］　Objective　To analyze the current status and imaging characteristics of multidrug-resistant Escherichia coli(MDR-ECO) pneumonia in hospital, and to explore the risk factors of MDR-ECO infection. Methods　The medical records of 178 patients with hospital acquired pneumonia(HAP) caused by Escherichia coli confirmed by the respiratory tract or blood sample isolation test in Weifang People′s Hospital from July 1, 2018 to June 30, 2020 were selected. The pathogenic bacteria were divided into MDR-ECO group(153 cases) and non-MDR-ECO group(25 cases) according to whether they were multidrug-resistant bacteria. Their drug resistance and imaging characteristics were analyzed. The risk factors of MDR-ECO infection were investigated by multivariate logistic regression analysis model. Results　One hundred and fifty-three strains of MDR-ECO specimens(85.96%) were detected in the 178 specimens. The results of drug susceptibility test showed that the drug resistance rates of MDR-ECO to cefazolin, cefuroxime and penicillins were more than 95%, and the drug resistance rates to gentamicin, aztreonam and ciprofloxacin were more than 50%. The drug resistance rates to ceftazidime and cefotaxime(the third-generation cephalosporins) were 46.41% and 83.01%, respectively, and the drug resistance rate to cefepime(the fourth-generation cephalosporin) was 30.07%. MDR-ECO had the lowest drug resistance rate to ertapenem(0.65%). Compared with the non-MDR-ECO group, the MDR-ECO group has more lesions involving both sides of the lungs, and higher proportions of patchy exudation, consolidation, interstitial changes, cavities and non-nodular lesions, and the differences were statistically significant between the two groups(P<0.05). Multivariate logistic regression analysis showed that co-existing underlying diseases and Intensive Care Unit(ICU) admission were the risk factors of MDR-ECO infection(P<0.05). Conclusion　The current status of drug resistance of HAP caused by MDR-ECO is severe. Clinicians should use antibiotics rationally according to the risk factors and imaging characteristics to reduce the production of drug-resistant bacteria.]]> 2021/3/5 17:41:47 LIU Ting, XU Jian-xiu, YANG Chun-li, et al. 587true ［Abstract］　Objective　To explore the clinical value of brainstem auditory evoked potentials(BAEP) in diagnosis of neonatal hyperbilirubinemia. Methods　From August 2017 to August 2019, ninety-eight newborns with hyperbilirubinemia admitted to Yangchun Maternal and Child Health Hospital were selected as the observation group, and 98 healthy newborns during the same period were selected as the control group. The detection of BAEP was performed in both groups and the results were compared. Results　There were 71 cases(72.45%) with abnormal BAEP in the observation group and 6 cases(6.12%) in the control group. There was significant difference in the BAEP abnormal rate between the two groups(χ²=90.374, P=0.000). The BAEP level and response threshold of the observation group were significantly higher than those of the control group in each period(P<0.05). The abnormal rate of BAEP in patients with severely abnormal serum bilirubin was significantly higher than that in patients with moderately and mildly abnormal serum bilirubin(P<0.05), and the abnormal rate of BAEP in patients with moderately abnormal serum bilirubin was also significantly higher than that in patients with mildly abnormal serum bilirubin(P<0.05). Conclusion　Application of BAEP has better clinical value in diagnosis of neonatal hyperbilirubinemia, which is conducive to the early understanding of the hearing and brainstem function impairment of the pediatric patients with neonatal hyperbilirubinemia, so as to perform the symptomatic treatment as early as possible.]]> 2021/3/5 17:41:47 LIN Fei-hong, LAN Shui-nong, CHEN Li-ming, et al. 586true ［Abstract］　Objective　To explore the clinical effect of blood recovery combined with low temperature protection technique in emergency operation for pregnant and lying-in women with massive hemorrhage in primary hospitals. Methods　Forty pregnant and lying-in women with massive hemorrhage who were admitted to Guiping People′s Hospital for emergency treatment were selected from January 2017 to December 2019. Among them, 20 cases were performed emergency surgery in primary hospitals(the research group), and the other 20 cases were performed emergency surgery in Guiping People′s Hospital(a city-level hospital)(the control group). The quality, function and morphology of recovered and washed red blood cell(RBC) were compared between the two groups, as well as the transfusion of allogeneic blood and the postoperative outcomes. Results　There were no significant differences in the amount of intraoperative blood loss and the amount of washed RBC between the two groups(P>0.05). There were no significant differences in the RBC count, hemoglobin(Hb), hematocrit(Hct), P₅₀(oxygen partial pressure when Hb oxygen saturation was 50%), K⁺ and Ca²⁺ of the recovered and washed RBC between the two groups(P>0.05). The bacterial culture results of the recovered and washed RBC were negative in both groups. Microscopically, the recovered and washed RBCs were uniform in size and shape, with a double concave disc shape, no rupture, no free hemoglobin(FHB), no fetal squamous cells, no vellus hair, no keratinized epithelium, no fetal fat and no other tangible substances in both groups. Eleven patients in the research group received allogeneic blood transfusion, while 3 patients in the control group received allogeneic blood transfusion, and the difference was statistically significant(P<0.05). The amount of allogeneic blood transfusion in the research group was greater than that in the control group, and the blood expenses in the research group were higher than those in the control group, and the differences were statistically significant between the two groups(P<0.05). There were no significant differences in the rescue success rate, postoperative fever incidence rate and patients′ satisfaction rate between the two groups(P>0.05). Conclusion　The combined blood recovery and low temperature protection technique can effectively recover the patients′ autologous blood during emergency operations in the department of obstetrics and gynecology of primary hospitals. The method is safe and effective and saves blood sources.]]> 2021/3/5 17:41:47 XU Yin-li, JIANG Li-ping, HUANG Chao-ying, et al. 585true ［Abstract］　Objective　To screen the key genes that affect the occurrence and development of ovarian cancer and to analyze their mechanisms of action. Methods　The key genes of ovarian cancer were screened by using Gene Expression Omnibus(GEO), the Cancer Genome Atlas(TCGA) and Kaplan-Meier Plotter(KMplot) databases, and their relationship with the patients′ clinicopathological characteristics was analyzed. At the same time, GESA was used for cluster analysis of signal pathways, and the TIMER database was used to identify the genes closely related to it. The interaction network was mapped through the STRING database to predict its mechanism of action. Results　A total of 13 differentially expressed genes were obtained through screening, among which Aristaless Related Homeobox(ARX) and Family with Sequence Similarity 150 Member B(FAM150B) were related to the prognosis of the patients, and the lower their expression levels, the shorter the overall survival time of the patients(P<0.01). Further analysis found that the expression levels of ARX and FAM150B were also related to the expression levels of interleukin-6(IL-6), Phosphoinositide-3-Kinase Regulatory Subunit 2(PIK3R2), PIK3CD, VAV1, PIK3CA, RAC2, PIK3R1, and MAPK1(P<0.05). The analysis of STRING database showed that ARX and FAM150B regulated the above genes through TUBA1A, EGFR and PLXNA2. Conclusion　The expression levels of ARX and FAM150B is low in ovarian cancer tissues, and their expression levels are closely related to the prognosis of the patients. ARX and FAM150B can regulate a series of genes, thereby weakening the body′s anti-tumor immune response and thus promoting the occurrence and development of ovarian cancer.]]> 2021/3/5 17:41:47 YU Zhan-peng, PENG Liang, JIANG Qiao 584true ［Abstract］　Objective　To investigate the protective effects of deep hypothermia on brain at subcellular level by studying the opening and closing situations of mitochondrial permeability transition pore(MPTP) in rat hippocampus after deep hypothermic circulatory arrest. Methods　Thirty-three adult Sprague-Dawley(SD) rats were taken. Three of them were used as the blood-supply animals and the other 30 rats were randomly divided into three groups: deep hypothermic circulatory arrest group(n=10), normal temperature circulatory arrest group(n=10) and normal control group(n=10). The cardiopulmonary bypass(CPB) rat model was established using closed-chest external intubation. Decapitation was performed on the rats and their bilateral hippocampal tissues were quickly removed from the brains to extract mitochondria, and then cytochrome C(CytC) in mitochondria was measured by Western blot to investigate the opening and closing situations of MPTP. Results　The opening degree of MPTP in the normal temperature circulatory arrest group was significantly greater than that in the normal control group and the deep hypothermic circulatory arrest group(P<0.05). The opening degree of MPTP in the normal control group was significantly less than that in the deep hypothermic circulatory arrest group(P<0.05). Conclusion　Ischemia and hypoxia can lead to the opening of MPTP. Deep hypothermia can inhibit the opening of MPTP, which ensures the normal metabolic activity of cells and thus plays a role in brain protection.]]> 2021/3/5 17:41:47 SHUAI Yong-xiao, LIU Jia, ZHANG Li-yin 583true ［Abstract］　Objective　To investigate the semen qualities of the patients with different types of infertility and their effects on the clinical outcomes of rescue intracytoplasmic sperm injection(R-ICSI). Methods　The clinical data of 98 infertile couples who failed in routine in vitro fertilization and embryo transfer(IVF-ET) and received R-ICSI treatment in the Center for Reproductive Medicine and Genetics, the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2014 to December 2018 were retrospectively analyzed. According to the types of infertility, the 98 couples were divided into primary infertility group(n=56) and secondary infertility group(n=42). The general conditions, semen quality, embryo development and clinical outcomes were compared between the two groups. Results　The sperm progressive motility in the secondary infertility group was higher than that in the primary infertility group, and the sperm DNA fragmentation index(DFI) in the secondary infertility group was lower than that in the primary infertility group, and the differences were statistically significant between the two groups(P<0.05). However, there were no significant differences between the two groups in terms of semen volume, sperm concentration, total number of sperm, normal sperm morphology rate, sperm acrosome integrity rate and sperm spontaneous acrosome reaction rate(P>0.05). There were no significant differences in the fertilization rate, cleavage rate, excellent embryo rate, live birth rate, premature birth rate, abortion rate, single birth rate and twin birth rate between the two groups(P>0.05). There was significant difference in the clinical pregnancy rate between the two groups(P<0.05). Conclusion　The semen quality of primary infertility patients is poor, and their clinical pregnancy rate is low. The types of infertility should be taken into consideration in diagnosis and treatment of reproductive assisted pregnancy.]]> 2021/2/3 9:17:59 CHEN Qi-gui, LI Da-wen, CHENG Jun-ping, et al. 582true ［Abstract］　Objective　To investigate the clinical effect of using static-progressive stretch splinting(SPSS) in treatment of stiff elbow patients after trauma surgery. Methods　Fifty-five stiff elbow patients after trauma surgery were selected from Shanghai Ninth People′s Hospital, Shanghai Jiao Tong University School of Medicine during June 2018 and May 2020. According to the voluntary use of SPSS, the patients were divided into the observation group(using SPSS, 28 cases) and the control group(not using SPSS, 27 cases). The control group was given conventional rehabilitation treatment(including physical therapy, manual therapy and exercise therapy), and the observation group received the same treatment as the control group plus the SPSS treatment. The elbow flexion angle, elbow extension angle, Mayo score and the duration of treatment were compared between the two groups before treatment, at the end of treatment and 6 months after treatment. Results　Before treatment, there were no significant differences in the elbow flexion angle, elbow extension angle and Mayo score between the two groups(P>0.05). At the end of the treatment and 6 months after the treatment, the elbow extension angle of the observation group was significantly smaller than that of the control group(P<0.05), while the elbow flexion angle and Mayo score of the observation group were significantly greater than those of the control group(P<0.05). There was no significant difference in the duration of treatment between the observation group and the control group［(8.3±3.2)weeks vs (9.6±3.6) weeks; t=1.417, P=0.163］. Conclusion　Use of SPSS is beneficial to improving the joint motion function of stiff elbow patients after trauma surgery.]]> 2021/2/3 9:17:59 FAN Shuai, CAI Bin 581true ［Abstract］　Objective　To explore the expressions of vascular endothelial growth factor(VEGF) in lymph nodes and bone marrow of mice with oral cancer at different stages, and the influences of their microenvironments on lymphatic metastasis of oral cancer. Methods　Forty 6-week-old male healthy Balb/c mice were selected and randomly divided into groups A, B, C, D, and E, with 8 mice in each group. The 4-nitroquinoline-1-oxide(4-NQO) drinking water method was used to construct a mouse oral cancer lymphatic metastasis model. Among them, group A was normal mice; group B had no pathological changes in the tongue mucosas of the mice, but there were already 4-NQO drug effects; group C had abnormal proliferation in the tongue mucosas of the mice, but no cancer had formed; group D had developed tongue cancer of the mice, but no transfer had taken place; group E had developed oral cancer of the mice, accompanied by mandibular lymph node metastasis. Immunohistochemistry and liquid chip methods were used to detect the expressions of VEGF in lymph nodes and bone marrow of the mice in different groups and the results were compared. The effects of lymph node and bone marrow microenvironments on the expressions of VEGF in the human tongue squamous cell carcinoma cells(SAS cells) in groups A, B, C and D were observed. Results　The results of VEGF immunohistochemical staining showed that VEGF expression was seen in the lymph nodes of the five groups, and the cytoplasmic staining was positive; however, the same bone marrow section under different visual fields was very different and difficult to analyze. The liquid chip method test results showed that for the lymph node tissue, the VEGF expression level of group A was significantly higher than that of group C and D(P<0.05), and the VEGF expression level of group E was significantly higher than that of group C(P<0.05), and there was no statistically significant difference between the other groups(P>0.05). For the bone marrow tissue, the VEGF expression levels of group A and group B were significantly higher than those of group C, group D and group E(P<0.05), and there was no statistically significant difference between the other groups(P>0.05). The expression levels of VEGF in the bone marrow tissue of the mice in the five groups were significantly higher than those in the lymph node tissue of the mice in the five groups(P<0.05). The results of co-cultivation experiments showed that in the microenvironments of lymph nodes and bone marrow in the periods of group A, group B and group C, the expression of VEGF in SAS cells showed an upward trend during the 24-72 h period. In the microenvironment of lymph nodes in the period of group D, the expression of VEGF in SAS cells showed a downward trend during the 24-72 h period, but in the microenvironment of bone marrow in the period of group D, the expression of VEGF in SAS cells showed an increased trend during the 24-72 h period. Conclusion　Lymph node and bone marrow microenvironments may affect the expression of VEGF in disseminated tumor cells, so as to achieve the targeting of oral cancer lymphatic metastasis.]]> 2021/2/3 9:17:59 LU Ying, CHENG Li, YAN Qi-zhang, et al. 580true ［Abstract］　Objective　To explore the effect of μ-opioid receptor gene polymorphism on pain sensitivity in patients undergoing lumbar spine surgery, and provide a theoretical basis for guiding individualized analgesia. Methods　Two hundred and eighteen patients undergoing lumbar spine surgery under general anesthesia in People′s Hospital of Xinjiang Uygur Autonomous Region from August 2018 to December 2019 were selected. According to OPRM1 A118G genotype, they were divided into wild-type homozygous group(AA group, n=85), mutant homozygous group(AG group, n=104) and mutant heterozygous group(GG group, n=29). The MEB-5100 electrical stimulator was used to detect the pain threshold and pain tolerance threshold of the three groups. The pain threshold and pain tolerance threshold were compared among the three groups before surgery, as well as the Numerical Rating Scale(NRS) scores at the time of rest and the time of activity 6 h, 24 h and 48 h after surgery. Results　The pain threshold and pain tolerance threshold of the AA group were significantly higher than those of the AG group and the GG group(P<0.05). However, there were no significant differences in the pain threshold and pain tolerance threshold between the AG group and the GG group(P>0.05). The pain threshold and pain tolerance threshold of male patients with the same genotype were significantly higher than those of female patients with the same genotype(P<0.05). In patients with GG genotype, the pain threshold and pain tolerance threshold of patients aged ≥50 years were significantly lower than those of patients aged 30-39 and 40-49 years(P<0.05). There were no significant correlations between pain threshold, pain tolerance threshold and age in patients with AA genotype and AG genotype(P>0.05). There were no significant differences in NRS scores among the three groups at the time of rest at the time points of 6 h, 24 h and 48 h after surgery(P>0.05). The NRS scores of the GG group at 6 h, 24 h, and 48 h time points after surgery were significantly higher than those of the AA group and the AG group(P<0.05), but there were no significant differences between the AA group and the AG group(P>0.05). The overall satisfaction of postoperative analgesia in the AA group and the AG group was significantly higher than that in the GG group(P<0.05). Conclusion　OPRM1 A118G gene polymorphism is associated with the body′s pain sensitivity and affects postoperative pain sensitivity in patients undergoing lumbar spine surgery.]]> 2021/2/3 9:17:59 YANG Hui-hong, LA Hong-ling, CHEN Nan, et al. 579true ［Abstract］　Objective　To explore the application value of lung ultrasound in the examination of neonatal atelectasis. Methods　Two hundred and eighty-six cases of neonatal atelectasis who were admitted to the Department of Neonatology of the Dehong People′s Hospital were selected from March 2018 to August 2020, including 121 cases of newborn respiratory distress syndrome(NRDS), 92 cases of pneumonia of newborn(PN), and 73 cases of meconium aspiration syndrome(MAS). All the patients received chest X-ray examination and lung ultrasound examination. Kappa consistency test was used to analyze the consistency of the two method results before treatment, on the 7th day after treatment, and on the 14th day after treatment. Results　Kappa consistency test results showed that before treatment, on the 7th day and 14th day of treatment, chest X-ray and lung ultrasound examination results of the NRDS, PN and MAS patients had high consistency(Kappa>0.60, P<0.05). Conclusion　Lung ultrasound can be used for the examination of neonatal atelectasis and is worthy of clinical application.]]> 2021/2/3 9:17:59 FENG Zai-li, YIN Zhao-qing, DUAN Zheng-fan, et al. 578true ［Abstract］　Objective　To explore the clinical application value of fetal heart ultrasound intelligent navigation technique in detecting fetal heart congenital malformations. Methods　Eleven cases of singletons diagnosed with fetal heart congenital malformations by fetal echocardiography were selected in Chengdu First People′s Hospital from July 2018 to December 2019. The fetal heart ultrasound intelligent navigation technique was used for inspection at 18.4～30.0(24.7±2.7) weeks of pregnancy, and the heart volume data of the fetuses with congenital heart malformations and the sonographic appearances of 9 fetal heart sections were analyzed and compared with those of the conventional fetal cardiac ultrasound. Results　Among the 11 fetuses with cardiac congenital malformations, there were 3 cases of vascular ring, 3 cases of truncus arteriosus, 1 case of double outlet right ventricle, 1 case of hypoplastic left heart syndrome, 1 case of pulmonary valve stenosis and 2 cases of simple ventricular septal defect. The fetal heart volume data were obtained in all the 11 cases. The fetal heart volume data of 1 case were unstable and difficult to analyze due to obvious fetal movement, while those of the other 10 cases can be analyzed offline using supporting software. The examination results of fetal heart ultrasound intelligent navigation technique showed that the sonograms of 9 fetal heart sections were grayscale images, no color blood flow imaging and no spectrogram, which could show or partially display the corresponding abnormal sonograms of the fetal heart congenital malformations. Conclusion　The success rate of obtaining fetal heart volume data by fetal heart ultrasound intelligent navigation technique is high. It can provide clinically valuable diagnostic information for fetal heart congenital malformations with obvious anatomical abnormalities. However, it can provide limited information for some cardiac malformations which need color flow mapping and spectral information to diagnose.]]> 2021/2/3 9:17:59 HU Li-rong, MA Xiao-juan, FENG Meng-juan, et al. 577true ［Abstract］　Objective　To compare the clinical effects of Zessys foraminoplasty and traditional foraminoplasty on treatment of distant prolapsed lumbar intervertebral disc herniation. Methods　The clinical data of 34 patients with distant prolapsed lumbar intervertebral disc herniation admitted to the Second Affiliated Hospital of Luohe Medical College from August 2017 to February 2019 were selected. Among them, 17 cases were treated with Zessys foraminoplasty(group A), and the other 17 cases were treated with traditional foraminoplasty(group B). The operation time, the number of fluoroscopy, and the amount of intraoperative blood loss were compared between the two groups. The Visual Analogue Scale(VAS) pain score, Oswestry Disability Index(ODI) score, and Japanese Orthopaedic Association(JOA) score were compared between the two groups before the operation, 3 days and 6 months after the operation. Results　The operation time of group A was shorter than that of group B, and the number of intraoperative fluoroscopy of group A was less than that of group B, with significant differences between the two groups(P<0.05). There was no significant difference in the amount of intraoperative blood loss between the two groups(P>0.05). The VAS and ODI scores 3 days and 6 months after the operation were significantly lower than those before the operation in the two groups(P<0.05), and the JOA scores 3 days and 6 months after the operation were significantly higher than those before the operation in the two groups(P<0.05). However, there were no significant differences in the VAS, ODI and JOA scores between the two groups before the operation and at different time points after the operation(P>0.05). Conclusion　Compared with traditional foraminoplasty, Zessys foraminoplasty can shorten the operation time and reduce the X-ray exposure to the doctors and patients during the operation in the treatment of distant prolapsed lumbar intervertebral disc herniation. Zessys foraminoplasty is worthy of clinical application widely.]]> 2021/2/3 9:17:59 WANG Song-mao, XU Yong-hui, HU Jun-hua, et al. 576true ［Abstract］　Objective　To study the correlation between extraction treatment and the changes in dental arch form, buccal corridor and facial profile in patients with skeletal class Ⅱ malocclusion of permanent dentition, and to provide reference for the extraction treatment of skeletal class Ⅱ malocclusion. Methods　Fifteen patients with permanent dentition type skeletal class Ⅱ malocclusion who underwent extraction treatment in the Department of Orthodontics, the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2013 to December 2017 were selected. The changes of the dental-maxillary craniofacial structures were analyzed by X-ray cephalometry. Vernier calipers were used to measure the changes of arch length and width of the maxillary model before and after extraction treatment. Adobe Photoshop CS6 software was used to measure and analyze the changes of frontal face image of the patients with smiling faces before and after extraction treatment. The changes in relevant indicators of craniomaxillofacial soft and hard tissues, width and length of the dental arch and buccal corridors of frontal face image of the patients with smiling faces and their correlation were analyzed before and after extraction treatment. Results　The SNA angle, ANB angle, U1-NA angle, UL-E and LL-E of the patients after extraction treatment were significantly decreased compared with those before treatment. The NLA angle of the patients after extraction treatment was significantly increased compared with that before treatment(P<0.05). Compared with those before treatment, the maxillary interpremolar width(UPP), maxillary posterior segment arch dental length(UPD), maxillary intermolar width(UMM), and maxillary total segment arch dental length(UAD+UPD) were all significantly reduced after treatment(P<0.05). Compared with those before treatment, the width of the bilateral cheilion on front smiling face(Ch to Ch), the sum of width of buccal corridor(BC) on front smiling face and the buccal corridor ratio(BCR) were increased, and the width level of visible dentition in maxilla(Va to Va) was decreased after treatment, but there were no significant differences in these indicators between prior-treatment and post-treatment(P>0.05). Pearson correlation analysis showed that the changes of UPP were negatively correlated with the changes of BC and BCR(P<0.05); the changes of UAD+UPD were positively correlated with the changes of UL-E(P<0.05); the changes of Ch to Ch were negatively correlated with the changes of U1-NA and UL- E(P<0.05); the changes of BC were positively correlated with SNB angle(P<0.05). Conclusion　After extraction treatment of skeletal class Ⅱ malocclusion, the changes of dental arch length are closely related to the improvement of profile convexity. A decrease in the dental mid-arch width may result in an increase in the buccal corridor of a patient with a frontal smile.]]> 2021/2/3 9:17:59 TU Zi-duan, ZHOU Yan, FANG Zhi-xin, et al. 575true ［Abstract］　Objective　To analyze the chromosomal karyotypes and pregnancy outcomes of fetuses with nuchal translucency(NT) thickening in the first trimester. Methods　From January 2019 to April 2020, 72 fetuses with NT thickening(NT≥2.5 mm) were selected in the Department of Eugenics and Genetics of Yulin Maternity and Child Health Hospital. All of them were confirmed by ultrasound examination in the first trimester, and the chorionic karyotypes were detected. The correlation between NT thickening and chromosomal karyotypes and fetal pregnancy outcomes was analyzed. Results　Of the 72 fetuses with thickened NT, 22 cases(30.56%) were detected with chromosomal abnormalities, among whom, 8 cases had trisomy 21(11.11%); 5 cases had trisomy 18(6.94%); 4 cases had 45, X(5.56%); 2 cases had trisomy 13(2.78%); 1 case had 47, XYY(1.39%); 1 case had balanced translocation(1.39%) and 1 case had chimera(1.39%). The abnormal karyotype rates in 2.5 mm≤NT<3.5 mm group, 3.5 mm≤NT<4.5 mm group and NT≥4.5 mm group were 16.67%(5/30), 24.00%(6/25) and 64.71%(11 /17), respectively, and the difference was statistically significant among the three groups(χ²=12.577, P=0.002). Chromosomal abnormalities were detected in 11 cases(20.37%) of the 54 patients with simple NT thickening, and chromosomal abnormalities were detected in 11 cases(61.11%) of the 18 patients with NT thickening and other ultrasonic abnormalities, and the difference was statistically significant between the two groups(χ²=10.560, P=0.001). Among the 72 cases of NT thickening fetus, 22 cases were terminated pregnancy and 50 cases were successfully delivered, among whom 1 case was found with a granulomatous neoplasm of 5 mm×5 mm in front of the right ear, and the rest survived without obvious adverse prognoses. Conclusion　Fetal NT thickening in early pregnancy is closely related to chromosomal abnormalities. The greater the thickness of NT, the higher the risk of chromosomal abnormalities in the fetus. When NT thickens and other ultrasound features are abnormal, the risk of fetal chromosomal abnormalities is higher.]]> 2021/2/3 9:17:59 LAI Yu-qing, DENG Guo-sheng 574true ［Abstract］　Objective　To explore the clinicopathological feature and the expression of programmed death-ligand 1(PD-L1) in lymphoepithelioma-like carcinoma(LELC). Methods　The clinical data of 15 cases of LELC diagnosed in Department of Clinical Pathology, the First Affiliated Hospital of Jinan University from January 2010 to May 2021 were collected. Immunohistochemical staining method was used to detect PD-L1, pan-cytokeratin(pan-CK), P40, leukocyte common antigen(LCA), synaptophysin(Syn) and Ki-67. In situ hybridization method was used to detect Epstein-Barr(EB) virus-encoded small RNA(EBER) in the tumor tissues. Results　Among the 15 LELC patients, there were 4 males and 11 females, aged from 28 to 81 years, with a median age of 59 years. The lesions were found in the lungs in 10 cases, in the salivary glands in 3 cases, in the renal pelvis in 1 case, and in the nasal cavity in 1 case. Under an optical microscope, the cancer cells were arranged in nests, diffuse sheets or strips; the cells were round, polygonal or irregular; the cytoplasm was red-stained, and the borders were unclear, undifferentiated or poorly differentiated; the nuclei were deeply stained or presented vaculating, with obvious nucleoli and mitotic figures; there were abundant lymphocytes and plasma cell infiltration in the stroma. The results of immunohistochemical staining showed that pan-CK and P40 were positive, and interstitial lymphocytes were positive for LCA; Ki-67 proliferation index ≥40% in 14 samples. Ten samples showed positive expression of PD-L1 membrane. Fourteen samples were positive for EBER. Conclusion　LELC is a relatively rare malignant tumor and is more common in the lungs. Most of the lesions are related to EB virus infection. In most cases, tumor cells highly express PD-L1, suggesting a possible benefit of immunotherapy.]]> 2022/1/6 12:24:24 ZHOU Ren-yu, ZHONG Li-juan, PENG Li-lin, et al. 573true ［Abstract］　Objective　To explore the mechanism of leptin inhibiting proliferation of Talaromyces marneffei(TM) in macrophages. Methods　THP-1 cells were cultured and induced to differentiate into THP-1 macrophages by stimulation of phorbol 12-myristate 13-acetate(PMA), and TM was add for co-culture, and the unswallowed TM spores were washed away. The experimental group was treated with leptin, and the control group was not treated with leptin. The colony forming unit(CFU) experiment was used to detect the proliferation of TM in THP-1 macrophages. Real-time fluorescent quantitative polymerase chain reaction(PCR) was used to detect the mRNA expression levels of Toll-like receptors(TLRs) and its downstream signal pathway factors. Results　The results of CFU experiment showed that leptin could effectively inhibit the proliferation of TM in THP-1 macrophages at a final concentration of 1-15 μg/ml(P<0.05), and the best inhibition effect could be obtained at a final concentration of 5 μg/ml. The results of real-time fluorescent quantitative PCR showed that the expression levels of TLR2 and TLR6 mRNA in the experimental group were higher than those in the control group, and the differences were statistically significant(P<0.05), but there were no significant differences in the expression levels of TLR3, TLR4, and TLR7 mRNA between the two groups(P>0.05). The expression levels of myeloid differentiation factor 88(MyD88), mitogen-activated protein kinase 1(MAPK1), interleukin-1β(IL-1β) mRNA in the experimental group were higher than those in the control group, and the expression level of IL-6 mRNA in the experimental group was lower than that in the control group, and the differences were statistically significant(P<0.05), but there were no significant differences in the expression levels of tumor necrosis factor α(TNF-α) and macrophage inflammatory protein 1α(MIP-1α) mRNA between the two groups(P>0.05). Conclusion　Leptin can inhibit the proliferation of TM in THP-1 macrophages, which may be achieved by up-regulating the TLR2/6 signaling pathway and activating inflammatory factors.]]> 2022/1/6 12:24:24 WEN Liu-fang, TANG Qiao, WANG Xin-wei, et al. 572true ［Abstract］　Objective　To observe the clinical effect of laparoscopic folding suture of the lower uterine segment combined with hysteroscopy on treatment of cesarean scar diverticulum(CSD). Methods　Twenty-six CSD patients admitted to Handan Central Hospital from January 2015 to December 2019 were selected and divided into the observation group(receiving hysteroscopic scar diverticulectomy combined with laparoscopic folding suture of the myometrium of the lower uterine segment to repair the myometrium of the lower uterine segment, 14 cases) and the control group(receiving laparoscopic excision of uterine scar and re-suturing to repair the myometrium of the lower uterine segment under the guidance of hysteroscope, 12 cases) according to different treatment methods. The operation time, intraoperative blood loss, postoperative hospital stay, and postoperative recovery of the myometrium of the lower uterine segment were compared between the two groups. Results　There was no significant difference in the operation time and postoperative hospital stay between the two groups(P>0.05). The intraoperative blood loss of the observation group was less than that of the control group, and the difference was statistically significant(P<0.01). Compared with that before operation, the thickness of the myometrium of the lower uterine segment increased significantly in the two groups after operation(P<0.01), but the difference was not statistically significant between the two groups(P>0.05). Conclusion　Laparoscopic folding suture of the lower uterine segment combined with hysteroscopy has a definite curative effect on treatment of CSD, and the operation is simpler and the amount of intraoperative blood loss is less. It is worthy of clinical promotion.]]> 2022/1/6 12:24:24 ZHANG Tong-yan, ZHANG Xue-qiang, ZHANG Ning, et al. 571true ［Abstract］　Objective　To explore the effects of Acorus calamus on neurotransmitter content in the brain of depression rats and their ethology. Methods　Thirty Sprague-Dawley(SD) rats were randomly divided into blank group, model group, fluoxetine group［1.8 mg/(kg·d)］, Acorus calamus low-dose group［5 ml/(kg·d)］, Acorus calamus middle-dose group［10 ml/(kg·d)］ and Acorus calamus high-dose group［20 ml/(kg·d)］, with 5 rats in each group. Except for the blank group, the other 5 groups were given chronic unpredictable mild stress(CUMS) to establish depression rat models. Forced swimming test(FST), open field test(OFT) and elevated plus-maze test(EPM) were used to compare the depressive symptoms among the 6 groups. The levels of dopamine(DA) and 5-hydroxytryptamine(5-HT) in brain tissues of rats were compared among the 6 groups. Results　The results of behavioral experiments showed that swimming immobility time of the model group was longer than that of the blank group, and that the OFT score and open-arm time(OT)/ total time(TT) of the model group were lower than those of the blank group, and the differences were statistically significant(P<0.05). The levels of DA and 5-HT in brain tissues in the model group were significantly lower than those in the blank group(P<0.05). The OFT score, swimming immobility time and OT/TT in the Acorus calamus high-dose group, Acorus calamus middle-dose group, Acorus calamus low-dose group, and the fluoxetine group improved compared with those in the model group, and the differences were statistically significant(P<0.05). With the increase of the dose of Acorus calamus, the levels of DA and 5-HT in brain tissues of rats showed an upward trend. The levels of DA and 5-HT in brain tissues in the Acorus calamus high-dose group and the fluoxetine group were significantly higher than those in the model group(P<0.05). However, compared with those in the blank group, the levels of DA and 5-HT in the Acorus calamus high-dose group and the fluoxetine group were not significantly different(P>0.05). Conclusion　Acorus calamus exerts an antidepressant effect by increasing the level of neurotransmitters in the brain tissues.]]> 2022/1/6 12:24:24 FU Jia, XUN Chen-xi, LI Shuang, et al. 570true ［Abstract］　Objective　To analyze the clinical and genetic characteristics of epilepsy related to SCN8A gene mutation. Methods　Eight children with SCN8A gene mutation-related epilepsy who were admitted to Department of Pediatrics, Fujian Provincial Hospital from January 2018 to December 2020 were selected, all of whom were verified by medical exome sequencing assay and Sanger sequencing. The patients′ clinical medical records and genetic testing results were collected for summary and analysis. Results　The median age of onset in the 8 pediatric patients was 6 months, including 6 males and 2 females. There were 7 types of epileptic seizures in these patients, of which 3 cases had more than 3 types of seizures. There were 5 cases of speech delay, 4 cases of hypotonia, 4 cases of ataxia, 2 cases of gait disorder, and 5 cases of mental retardation. Except for 1 case with normal electroencephalogram(EEG), the other 7 cases had abnormal interictal EEG, mainly multifocal discharge. All the 8 cases had missense mutations, among whom 4 cases had unreported mutations. Five cases had de nove mutations and 3 cases had genetic heterozygous mutations, and their clinical phenotypes were different. The phenotypes of the 3 mutations located in the C-terminalnd of the domain were also different. Conclusion　SCN8A gene-related epilepsy is a pedigree disease, and the de nove mutation may show mild to severe epilepsy encephalopathy, and the hereditary heterozygous mutation may also show epilepsy encephalopathy. There is no correlation between mutation mode and phenotype. The distribution of mutation sites in the domain has no correlation with mutation source and clinical phenotype.]]> 2022/1/6 12:24:24 LIN Meng, CHEN Lang, LIN Quan, et al. 569true ［Abstract］　Objective　To observe the clinical efficacy of catheter-directed thrombolysis(CDT) via anterior tibial vein approach in treatment of acute deep venous thrombosis(DVT) of lower extremities. Methods　Forty-three patients with acute DVT of lower extremities(onset within 1 week) were selected from No.2 People′s Hospital of Fuyang City during January 2019 and March 2020 to receive CDT treatment via anterior tibial vein approach. The difference of lower limb circumference between healthy side and affected side was compared before and after treatment. The complications and clinical efficacy were observed in the patients after treatment. Results　Thirty-nine cases(90.70%) were successfully catheterized through the anterior tibial vein approach. There were 5 cases of mild pain, and 3 cases of numbness and discomfort but no cases of bleeding complications at the puncture site after the operation. After the operation, 32 cases were followed up for 3-12 months and their symptoms disappeared during the follow-up period, and there was no recurrence of thrombosis and pulmonary embolism. After treatment, the difference of lower limb circumference between the patients′ healthy side and affected side was significantly reduced(P<0.05). The venous patency scores after treatment［(3.29±0.91)points］ were lower than those before treatment［(8.47±1.09)points］, and the differences were statistically significant(t=19.475, P=0.000). The average venous patency rate was (57.24±11.57)%, and the valve preservation rate reached 84.38%(27/32) after treatment. Conclusion　CDT via the anterior tibial vein approach has a good clinical effect on the treatment of acute DVT of lower extremities, and there are few surgical complications, and it is worthy of clinical application.]]> 2022/1/6 12:24:24 SUN Zhen-yang, RUI Qing-feng 568true ［Abstract］　Objective　To investigate the effects of closed-loop target-controlled infusion of propofol combined with etomidate and different doses of remifentanil induction on intubation response in hypertensive patients. Methods　Ninety hypertensive patients receiving endoscopic surgery for endotracheal intubation under general anesthesia in Affiliated Minzu Hospital of Guangxi Medical University from January 2019 to March 2020 were selected, and they were divided into 4 ng/ml remifentanil group(R1 group), 5 ng/ml remifentanil group(R2 group) and 6 ng/ml remifentanil group(R3 group) by random number table method, with 30 cases in each group. The double-channel anesthesia depth closed-loop controlled injection system was connected. After the effect-chamber concentration of remifentanil reached 2 ng/ml, etomidate(0.15 mg/kg) was rapidly injected intravenously and then the plasma target-controlled closed-loop of propofol(1.5 μg/ml) was started. Vecuronium(0.1 mg/kg) was injected intravenously in the three groups after the cerebral state index(CSI) was below 60. The target concentrations of remifentanil were set as 4 ng/ml in the R1 group, 5 ng/ml in the R2 group and 6 ng/ml in the R3 group, respectively, two minutes before tracheal intubation. Tracheal intubation was performed three minutes after the vecuronium bolus injection in all the three groups, and the target concentrations of remifentanil were adjusted to 2 ng/ml after intubation. The mean arterial pressure(MAP) and heart rate(HR) were compared among the three groups at the time of entering into the operating room(T₀), the time of loss of consciousness(T₁), the time of tracheal intubation(T₂), 1 minute after tracheal intubation(T₃) and 5 minutes after tracheal intubation(T₄). The time of loss of consciousness and the effect-site concentration and dosage of propofol at T₁ and T₂ time points were compared among the three groups. The occurrence of adverse reactions and the application of vasoactive drugs in the three groups were recorded. Results　There were no statistically significant differences in the time of loss of consciousness and the effect-site concentration and dosage of propofol at T₁ and T₂ time points among the three groups(P>0.05). Within the same observation time point, MAP in the three groups showed a downward trend with the increase of remifentanil dose, but there was no significant difference in the trend of changes among the three groups(P>0.05). There was no significant difference in HR among the three groups(P>0.05). The incidence of intubation reaction in the R1 group was significantly higher than that in the R2 and R3 groups(P<0.05). The incidence of blood pressure reduction in the R3 group was significantly higher than that in the R1 group(P<0.05). There were no significant differences in the increase of blood pressure, the application of nitroglycerin, and the application of phenylephrine among the three groups(P>0.05). Conclusion　The induced sedative effects of closed-loop target-controlled infusion of propofol combined with etomidate and three groups of remifentanil are satisfactory. Remifentanil at the dose of 5 ng/ml is more suitable for tracheal intubation in hypertensive patients, and it has good sedative effect, stable hemodynamics and less intubation response.]]> 2022/1/6 12:24:24 FENG Yan-chun, HUANG Ge, RUAN Hao-shen, et al. 567true ［Abstract］　Objective　To observe the clinical effect of Jiawei Zhishu Powder treated by way of pasting on Shenque acupoint in assisted treatment of antibiotic-associated diarrhea(AAD) in children. Methods　Eighty AAD children admitted to Lianjiang People′s Hospital from January 2020 to January 2021 were selected and divided into observation group and control group by random number table method, with 40 cases in each group. The control group was treated with Saccharomyces boulardii+montmorillonite powder, and the observation group received the same treatment as the control group plus Jiawei Zhishu Powder treated by way of pasting on Shenque acupoint. The degree of improvement in diarrhea was compared between the two groups. Results　In the observation group, 13 cases were markedly effective, 25 cases effective, and 2 cases ineffective. In the control group, 10 cases were markedly effective, 22 cases effective, and 8 cases ineffective. The total effective rate of the observation group was significantly higher than that of the control group(95.00% vs 80.00%; χ²=4.114, P=0.042). After the intervention, the frequency of diarrhea in the two groups was significantly lower than that before the intervention(P<0.05), and the frequency of diarrhea in the observation group was lower than that in the control group, and the difference was statistically significant(P<0.01). The duration of diarrhea in the observation group was shorter than that in the control group, and the difference was statistically significant(P<0.01). Conclusion　Jiawei Zhishu Powder treated by way of pasting on Shenque acupoint is markedly effective in assisted treatment of ADD children, and is worthy of recommendation to use widely in clinic.]]> 2022/1/6 12:24:24 ZHONG Wei-en, YE Hong, TAN Yi-qiu, et al. 566true ［Abstract］　Objective　To investigate the value of prenatal blood D-dimer level in predicting adverse pregnancy outcomes. Methods　The data of medical records of 612 pregnant women admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2019 to June 2019 were retrospectively analyzed, among whom 263 pregnant women with adverse pregnancy outcomes were selected as the study group, and 349 pregnant women with good pregnancy outcomes were selected as the control group. The prenatal level of blood D-dimer was compared between the two groups, and in the patients with adverse pregnancy outcomes, the prenatal levels of blood D-dimer were further compared among the postpartum hemorrhage group, placental abruption group, severe preeclampsia group and other groups(including preeclampsia except severe preeclampsia, hypertension during pregnancy, premature rupture of membranes, premature delivery, infants small than gestational age and neonatal asphyxia). The value of prenatal blood D-dimer in prediction of adverse pregnancy outcomes was analyzed by using receiver operating characteristic(ROC) curve. Results　The prenatal blood D-dimer level of the study group was significantly higher than that of the control group(P<0.05). The prenatal blood D-dimer levels of other groups were significantly lower than those of the postpartum hemorrhage group, the placental abruption group and the severe preeclampsia group(P<0.05); there was no significant difference in the prenatal blood D-dimer level among the postpartum hemorrhage group, the placental abruption group and the severe preeclampsia group(P>0.05). The results of ROC curve analysis showed that the area under the ROC curve for prenatal blood D-dimer to predict adverse pregnancy outcomes was 0.743(95%CI: 0.706-0.777, P=0.000); the cut-off value was 1.41 mg/L, whose corresponding sensitivity and specificity were 80.20% and 53.93%, respectively. Conclusion　The prenatal level of blood D-dimer can be used as an indicator to predict adverse pregnancy outcomes. For the pregnant women with abnormally high prenatal levels of blood D-dimer, more attention should be paid to the occurrence of postpartum hemorrhage, placental abruption and severe preeclampsia.]]> 2022/1/6 12:24:24 WEI Hai-tang, WU Hua, GONG Tian-liu 565true ［Abstract］　Objective　To analyze the effects of the weight gain of pregnant women before pregnancy and in the second and third trimesters of pregnancy on neonatal birth weight. Methods　A total of 1 820 pregnant women who were created medical files for giving birth and underwent routine perinatal examinations and gave birth at Tengzhou Maternal and Child Health Hospital, Shandong Province from October 2016 to October 2020 were prospectively selected. The data on basic demographic characteristics, antenatal information and pregnancy outcomes were collected. The binary logistic regression model was used to analyze the correlation between the pre-pregnancy body mass index(BMI) and the weight gain of pregnant women in the second and third trimesters of pregnancy and the birth weight of newborns. Results　The low weight of pregnant women before pregnancy(OR=6.089, 95%CI: 4.075-9.099) was the risk factor of low weight infants. Overweight(OR=5.799, 95%CI: 3.895-8.636) and obesity(OR=11.779, 95%CI: 7.096-19.554) of pregnant women before pregnancy were the risk factors of macrosomia. The low Institute of Medicine(IOM) criteria of weight gain in the second trimester(OR=6.261, 95%CI: 3.854-10.170) and the third trimester(OR=6.926, 95%CI: 4.495-10.672) of pregnancy were the risk factors for the occurrence of low weight infants. The excess IOM criteria of weight gain in the second trimester(OR=1.496, 95%CI: 1.059-2.113) and the third trimester(OR=1.812, 95%CI: 1.279-2.567) of pregnancy were the risk factors for the occurrence of macrosomia. Conclusion　The pre-pregnancy BMI and the rates of weight gain in the second and third trimesters have a greater impact on fetal development. Clinical targeted interventions should be given to pregnant women at specific stages according to their physical fitness to ensure the reasonable weight gain during pregnancy and further improve pregnancy outcomes.]]> 2022/1/6 12:24:24 HU Xiao-qin 564true ［Abstract］　Objective　To explore the correlation between perioperative thromboelastography(TEG) parameters and the indexes of coagulation function, inflammation and hemorheology in patients with traumatic closed fracture of lower extremity. Methods　The clinical data of 81 patients with traumatic closed fracture of lower extremity admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region from November 2018 to July 2020 were selected. Pearson correlation analysis was used to explore the correlation between TEG parameters(R value, K value, α angle, MA value) and the indexes of coagulation function［prothrombin time(PT), activated partial thromboplastin time(APTT), fibrinogen(FIB), platelet(PLT)］, inflammation［C-reactive protein(CRP), white blood cell(WBC)］ and hemorheology［erythrocyte sedimentation rate(ESR), hematocrit(HCT)］. Results　Among the 81 patients, there were 68 cases with MA value ≥68 mm, and the incidence of hypercoagulability was 83.95%. R value was significantly positively correlated with APTT(r=0.287), FIB(r=0.310) and ESR(r=0.300). K value was significantly negatively correlated with PLT(r=-0.318), and significantly positively correlated with HCT(r=0.471). α angle was significantly positively correlated with PLT(r=0.347) and ESR(r=0.258), and significantly negatively correlated with HCT(r=-0.431). MA value was significantly positively correlated with APTT(r=0.319), FIB(r=0.550), PLT(r=0.626), CRP(r=0.351) and ESR(r=0.691), and significantly negatively correlated with HCT(r=-0.420). Conclusion　The perioperative TEG parameters of patients with lower limb fracture have a certain correlation with the indexes of blood coagulation function, inflammation and hemorheology, which can provide reference for clinical evaluation of the patients′ conditions.]]> 2022/1/6 12:24:24 LIAO Xiang-cheng, SUN Ke, MO Zhu-ning, et al. 563true ［Abstract］　Objective　To explore the relationship between prenatal ultrasound diagnosis of fetal limb malformations(FLM) and chromosomal abnormalities. Methods　Two hundred and thirty fetuses diagnosed as FLM by prenatal ultrasonography at Maternal and Child Health Hospital of Inner Mongolia Autonomous Region from January 2017 to December 2019 were selected, and 56 fetuses′ mothers voluntarily underwent chromosome examination. The results of prenatal ultrasonography were summarized and the relationship between the results of prenatal ultrasonography and chromosomal abnormalities was analyzed. Results　Among the 230 cases of FLM, 191 cases(83.04%) had single limb deformity and 39 cases(16.96%) had compound limb deformity. Limb deformities were found in 55 cases(18.27%, 55/301) in 14-19 gestational weeks and 208 cases(69.10%, 208/301) in 20-24 gestational weeks.Ninety-six cases(41.74%) were complicated with malformations of other systems, most of which were complicated with malformations of two or more other systems. The total number of FLM diagnosed by ultrasound in this study reached 301 cases. After induced labour and delivery, 32 cases were confirmed to be missed and 7 cases were misdiagnosed. Polydactyly(toe) accounted for the highest proportion of missed deformities(40.63%); 7 cases were misdiagnosed, including 3 cases of varus foot misdiagnosis and 4 cases of abnormal hand posture, and all of these abnormalities were transient. The results of chromosome examination in 56 cases showed that 31 cases had chromosomal abnormalities, including 15 cases of trisomy 18, 9 cases of trisomy 21, and 7 cases of other types of chromosomal abnormalities. There were 9 cases of overlapping fingers in the 18-trisomy FLM, and all the 18-trisomy FLM were complicated with other system malformations. Phalange loss was common in the 21-trisomy FLM(8 cases). Conclusion　Prenatal ultrasound in the second trimester of pregnancy has a high detection rate for FLM, but it is still difficult to detect minor deformities such as polydactyly(toe) and syndactyly(toe). FLM has a certain correlation with chromosomal abnormalities and can be used as an indicator of fetal invasive chromosome screening.]]> 2022/1/6 12:24:24 LI Xin-ze, LIU Guo-hui, LIU Min, et al. 562true ［Abstract］　Objective　To construct a model of fatty degeneration of hepatoblastoma cell line(HepG2) cells induced by oleic acid, and to explore the mechanisms of silibinin in regulating lipid deposition in HepG2 cells. Methods　HepG2 cells were stimulated by 0.4 mM oleic acid for 24 hours to construct a steatosis model. The control group, the oleic acid group(OA group), the oleic acid+silibinin group(OA+S group), the oleic acid+chloroquine group(OA+CQ group) and the oleic acid+chloroquine+silibinin group(OA+CQ+S group) were set up according to different experimental purposes, with 4 samples in each group. Oil red O staining and triglyceride(TG) detection were used to evaluate the lipid deposition of cells in each intervention group. Western blot was used to detect the expression of autophagy marker protein LC3B in each intervention group. Results　The number of intracellular stained lipid droplets in HepG2 cells in the OA group was significantly more than that in the control group, and the TG content in the OA group was higher than that in the control group, and the differences were statistically significant(P<0.05). In the OA+S group, the number of intracellular stained lipid droplets decreased after the intervention of silibinin, and the content of TG decreased, which were significantly different compared with those in the OA group(P<0.05). The OA+CQ+S group was pre-treated with autophagy inhibitor chloroquine for 1 hour after stimulation with oleic acid, and then added with silibinin for 24 hours. Significant lipid deposition occurred and TG content increased in HepG2 cells, and there were statistically significant differences between the OA+CQ+S group and the OA+S group(P<0.05), but the changes of lipid deposition and the increase of TG content in the OA+CQ+S group were similar to those in the OA group(P>0.05). The results of Western blot detection showed that the level of LC3BⅡ/LC3BⅠ in the OA group was lower than that in the control group(P<0.05), and the level of LC3BⅡ/LC3BⅠin the OA+S group was significantly higher than that in the OA group, and there was no significant difference in the level of LC3BⅡ/LC3BⅠbetween the OA+S group and the control group(P>0.05). The levels of LC3BⅡ/LC3BⅠin the OA+CQ group and the OA+CQ+S group were significantly higher than those in the OA group and the OA+S group(P<0.05). Conclusion　0.4 mM oleic acid can cause fatty degeneration of HepG2 cells. Silibinin improves oleic acid-induced lipid deposition by enhancing autophagy in HepG2 cells.]]> 2021/12/3 11:58:03 XIAO Su-jun, XIAO Ya-jun, WU Pei-sai, et al. 561true ［Abstract］　Objective　To investigate the mechanism of action of Qingjie Huagong Prescription(QJHGP) in treating severe acute pancreatitis(SAP) based on network pharmacology and molecular docking technology. Methods　Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP) was used to retrieve the active components and action targets of QJHGP. Cytoscape was used to construct the network diagram of “drug-component-target-disease”. DAVID database and R software were used to carry out GO and KEGG enrichment analysis on the intersection genes of drugs and diseases. Autodock Vina software was used for molecular docking. Results　A total of 202 active components and 225 potential targets were screened out in QJHGP. Among the 225 potential targets, 210 targets were related to SAP. The results of target pathway enrichment analysis showed that QJHGP might play a role in acute pancreatitis by intervening in inflammatory factors, apoptosis and other biological processes. The results of molecular docking showed that the main active components of QJHGP had high binding activity with the key targets. Conclusion　QJHGP may play the functions of anti-inflammatory, regulation and control of cell apoptosis and improvement of energy metabolism through p53 signal pathway, PI3K Akt signal pathway and other pathways.]]> 2021/12/3 11:58:03 QIN Bai-jun, JIANG Liu-qing, CHEN Rui, et al. 560true ［Abstract］　Objective　To observe the clinical efficacy of vitamin D receptor activator(VDRA) combined with nutritional vitamin D(NVD) in the treatment of idiopathic membranous nephropathy(IMN) and to provide reference for clinical treatment of IMN. Methods　Sixty patients with new-onset IMN and 25-hydroxy vitamin D［25(OH)D］<75 nmol/L who were admitted to the Department of Nephrology, the People′s Hospital of Guangxi Zhuang Autonomous Region from September 2017 to September 2019 were selected. The patients were divided into VDRA group(34 cases) and VDRA combined with NVD group(26 cases) according to their willingness to accept different treatment regimens. The levels of 25(OH)D, 24-hour urinary protein quantity(24h up), albumin(ALB), creatinine(Cr), estimated glomerular filtration rate(eGFR), total cholesterol(TC), triglyceride(TG) and blood calcium(Ca) were compared between the two groups before treatment, 3 months of treatment, and 6 months of treatment. The clinical efficacy and the occurrence of adverse events were compared between the two groups. Results　After treatment, the level of 25(OH)D showed an upward trend in both groups, and the level of 25(OH)D in the VDRA combined with NVD group increased more significantly than that in the VDRA group(P<0.05). After 3 months and 6 months of treatment, the level of 25(OH)D in the VDRA combined with NVD group was higher than that in the VDRA group, and the difference was statistically significant(P<0.05). After treatment, the 24h up of the VDRA combined with NVD group and the 24h up of VDRA group showed a downward trend, and the change of the VDRA combined with NVD group was more significant(P<0.05). ALB showed an upward trend, and TC showed a downward trend in the VDRA combined NVD group, but ALB and TC in the VDRA group did not change much, and the difference between the two groups was statistically significant(P<0.05). Cr and Ca showed an upward trend, and eGFR showed a downward trend in the VDRA combined NVD group and the VDRA group, and the difference between the two groups was not statistically significant(P>0.05).The level of TG did not change much in the two groups during the treatment period(P>0.05). There was no recurrence in both groups during the treatment period. In the treatment of 3 months and in the treatment of 6 months, the total remission rates of the VDRA combined with NVD group were higher than those of the VDRA group during the same period, and the differences were statistically significant(P<0.05). There were 21 cases of fatigue in the VDRA group during the treatment period, and 7 cases in the VDRA combined NVD group, and the difference was statistically significant(P<0.05). Conclusion　VDRA combined with NVD in the treatment of IMN can effectively reduce the patients′ 24h up and increase the 25(OH)D level. Its clinical efficacy is better than that of VDRA alone, and its safety is good.]]> 2021/12/3 11:58:03 ZENG Yuan-yuan, YE Kun, LIAO Xin, et al. 559true ［Abstract］　Objective　To analyze the types, distribution characteristics and drug resistance of pathogens in patients with bacterial infections in the Department of Hematology of Guangzhou First People′s Hospital during January 2010 and December 2019 to provide reference for clinical anti-infection treatment. Methods　The medical records of 1 221 patients who had bacterial infections in the Department of Hematology, Guangzhou First People′s Hospital from January 2010 to December 2019 were collected. The patients′ disease types included the hematology systemic and non-hematology systemic diseases. The types, distribution characteristics and drug resistance of the pathogens were statistically analyzed, and the differences in infections of the pathogens between patients with severe aplastic anemia(SAA) and those with hematological malignancies were compared. Results　Among the 1 221 strains of pathogens, 803 strains(65.77%) were gram-negative bacteria and 418 strains(34.23%) were gram-positive bacteria. The top three gram-negative bacteria were Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa. The top three gram-positive bacteria were Enterococcus faecium, Staphylococcus haemolyticus and Staphylococcus aureus. The detection rates of extended-spectrum β-lactamases(ESBLs) in Escherichia coli and Klebsiella pneumoniae were 60.87% and 58.03%, respectively, and their resistance rates to imipenem were 3.38% and 4.66%, respectively. From 2010 to 2019, the detection rate of carbapenem-resistant Enterobacteriaceae(CRE) showed an overall upward trend. Antibiotics with lower resistance to Pseudomonas aeruginosa included imipenem, meropenem, ceftazidime, cefepime, piperacillin-tazobactam, aminoglycosides and quinolones. The detection rates of imipenem-resistant Pseudomonas aeruginosa(IRPA) and imipenem-resistant Acinetobacter baumannii(IRAB) were 17.74%(22/124) and 13.64%(6/44), respectively. Antibiotics with lower resistance to Stenotrophomonas maltophilia included ceftazidime, levofloxacin and compound sulfamethoxazole. In this study, the detection rate of methicillin-resistant Staphylococcus aureus(MRSA) was 20.59%(14/68), and vancomycin and linezolid-resistant Staphylococcus aureus strains were not detected. The detection rate of methicillin-resistant coagulase negative Staphylococcus(MRCNS) was 90.28%(130/144). Both Enterococcus faecalis and Enterococcus faecium had lower resistance rates to vancomycin and linezolid. Compared with those in the SAA patients, the incidence rates of Enterococcus faecium and Staphylococcus aureus infections were higher in the hematological malignancies group(P<0.05). Conclusion　Inpatients in hematology department have a high incidence of infections, among whom the patients with SAA and hematological malignancies are particularly common. Gram-negative bacteria are the main pathogens of the infections in patients with blood diseases. For patients suffering from hematological diseases complicated with infections, etiological examination should be carried out in time. Clinicians can conduct empirical anti-infection treatments according to the characteristics of the bacterial pathogen types and drug resistances in the hospital.]]> 2021/12/3 11:58:03 ZHOU Wei, XU Shi-lin, DONG Mei-hua, et al. 558true ［Abstract］　Objective　To analyze the ultrasonic manifestations of 123 cases of granulomatous lobular mastitis(GLM), and to explore the clinical application value of ultrasound in the diagnosis of GLM disease. Methods　The clinical data of 123 GLM patients admitted to the Department of Thoracic Breast, Xiamen Hospital of Beijing University of Chinese Medicine from January 2018 to October 2020 were retrospectively analyzed. All the patients were diagnosed as confirmed cases of GLM by needle biopsy or surgical pathology. The ultrasonic manifestations of GLM were classified as nodular type(13 cases), mass type(18 cases), abscess type(33 cases), and sinus hypoechoic zone type(59 cases) according to the ultrasound images, and the differences in the ultrasonic manifestations of each type were analyzed. Results　The four types had statistically significant differences in the ultrasonic manifestations of the enhanced surrounding echoes of the lesion, abundant blood flow signals, sinus tracts, axillary lymphadenopathy, and skin involvement(P<0.05). Among them, the nodular type was mainly manifested as abundant blood flow signals; the mass type was mainly manifested as abundant blood flow signals and axillary lymphadenopathy; the abscess type and the sinus hypoechoic zone type were mainly manifested as the enhanced surrounding echoes of the lesion, abundant blood flow signals, and axillary lymphadenopathy. Conclusion　The ultrasonic manifestations of different types of GLM are quite different, which can provide a reference for the diagnosis and treatment of clinical diseases.]]> 2021/12/3 11:58:04 LIN Yun, LIN Yun, CHANG Jian-dong, et al. 557true ［Abstract］　Objective　To investigate the association between serum galectin-3(Gal-3) and new-onset atrial fibrillation(AF) in patients with acute myocardial infarction(AMI). Methods　From October 2018 to December 2019, 30 patients with AMI complicated with new-onset AF who were admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region were selected as the new-onset AF group, and 30 patients with AMI but without new-onset AF during the same period were selected as the non-AF group. The clinical data of the two groups were collected through the hospital medical record system. Multivariate logistic regression analysis was used to analyze the factors influencing new-onset AF in the AMI patients, and Pearson correlation analysis was used to investigate the correlation between Gal-3 and N-terminal prohormone of brain natriuretic peptide(NT-proBNP) and the anteroposterior diameter of the left atrium. Results　The anteroposterior diameter of the left atrium, the levels of NT-proBNP and serum Gal-3 in the new-onset AF group were higher than those in the non-AF group, and the differences were statistically significant between the two groups(P<0.05). The results of multivariate logistic regression analysis showed that a higher level of serum Gal-3 was a risk factor for the development of new-onset AF in the AMI patients(OR=2.719, P=0.006). The levels of serum Gal-3 in the AMI patients were significantly positively correlated with the levels of NT-proBNP and the anteroposterior diameter of the left atrium(P<0.05). Conclusion　Serum Gal-3 level is closely related to new-onset AF in patients with AMI, and has the potential to be used as a marker for predicting new-onset AF in AMI patients.]]> 2021/12/3 11:58:04 LI Qing-kuan, QIN Shao-ming, LU Zhi-hong, et al. 556true ［Abstract］　Objective　To observe the effect of pranoprofen combined with mydrin-P on the size of pupil in vitrectomy for proliferative diabetic retinopathy. Methods　Eighty-nine patients(126 eyes) who underwent vitrectomy at Department of Ophthalmology, Guiyang First People′s Hospital from April 2020 to May 2021 due to proliferative diabetic retinopathy were divided into the observation group(63 eyes) and the control group(63 eyes) by random number table method. The observation group started to use pranoprofen on the first day after anti-vascular endothelial growth factor(VEGF) operation for 3 consecutive days, and mydrin-P was used to dilate the pupils half an hour before the operation. In the control group, after anti-VEGF operation, mydrin-P was used to dilate the pupils only half an hour before operation. The pupil size was measured before operation, 1 hour during the operation, and at the end of the operation(≥2 h), and the size of average pupil area at each time point was compared between the two groups. Results　The average pupil area of the observation group at each time point was greater than that of the control group, and the difference was statistically significant(P<0.05). Compared with those before operation, there were no statistical differences in average pupil areas 1 hour during the operation in the two groups(P>0.05). The average pupil areas at the end of the operation(≥2 h) were lower than those before operation in both groups, and the differences were statistically significant(P<0.05), but the degree of reduction in average pupil area in the observation group was obviously smaller than that in the control group, and the difference was statistically significant(P<0.05). Conclusion　Pranoprofen can effectively inhibit myosis during vitrectomy for proliferative diabetic retinopathy. Pranoprofen in combination with mydrin-P can maintain mydriasis for a long time during the operation and creates a good field of vision for the operation.]]> 2021/12/3 11:58:04 YAN Ai-min, QIU Xu, PENG Kai, et al. 555true ［Abstract］　Objective　To evaluate the efficacy of arthroscopy combined with Broström-Gould procedure in treatment of chronic ankle instability(CAI). Methods　The clinical data of 30 patients with CAI in Hubei 672 Orthopedic Hospital of Integrated Chinese and Western Medicine from July 2018 to July 2019 were collected. All the patients received arthroscopy combined with Broström-Gould procedure treatment. Visual Analogue Scale(VAS) score, American Orthopaedic Foot and Ankle Society(AOFAS) ankle and hindfoot total score, Karlsson-Peterson ankle function score and talar tilt angle in the patients were compared before operation, and 6 weeks and 12 months after operation. Results　All the 30 patients were followed up for 12 to 22 months, with a mean period of (16.0±3.1)months. The postoperative talar tilt angle decreased compared with the preoperative talar tilt angle. The postoperative VAS pain score gradually decreased. The postoperative Karlsson-Peterson ankle function improved compared with the preoperative Karlsson-Peterson ankle function, and the postoperative AOFAS ankle and hindfoot total score improved significantly compared with the preoperative AOFAS ankle and hindfoot total score, with statistically significant differences(P<0.05). Conclusion　Arthroscopy combined with Broström-Gould procedure has a satisfactory effect on treatment of CAI, and the recovery of ankle joint function is good.]]> 2021/12/3 11:58:04 WANG Wei, LI Zhao-wen, ZUO Chang-jun, et al. 554true ［Abstract］　Objective　To investigate the expression of NADH dehydrogenase(ubiquinone) 1 alpha subcomplex 4-like 2(NDUFA4L2) in gliomas and its clinical significance. Methods　The Cancer Genome Atlas(TCGA) and Chinese Glioma Genome Atlas(CGGA) databases were used to analyze the mRNA expression of NDUFA4L2 in glioma and its relationship with the clinical pathological grade and prognosis of the patients. The glioma tissue specimens of different grades were further collected for immunohistochemical detection to verify the difference of protein expression of NDUFA4L2 in different grades of gliomas. The tussue specimens and follow-up data of 62 patients with glioma were collected. Real-time quantitative polymerase chain reaction(RT-PCR) method was used to detect the expression of NDUFA4L2 in glioma and its relationship with prognosis was analyzed. Results　By analyzing the TCGA and CGGA databases, it was found that the expression of NDUFA4L2 in glioma tissues increased with the increase of the tumor grade(P<0.05). High expression of NDUFA4L2 indicated a shorter survival period for the patients. The results in the clinical specimen verification were consistent with the above-mentioned bioinformatic analysis. Conclusion　The expression of NDUFA4L2 in glioma tissues is related to the pathological stage and prognosis, and NDUFA4L2 may be a potential tumor marker of glioma.]]> 2021/12/3 11:58:04 LI Chun-ya, CHEN Yuan-bing, LIANG Yin-hua, et al. 553true ［Abstract］　Objective　To explore the nutritional status of vitamin D in non-manual workers and retirees, and to analyze its correlation with glucose and lipid metabolism. Methods　The data of 494 subjects who underwent physical examination at the Physical Examination Center of the People′s Hospital of Guangxi Zhuang Autonomous Region from May 2019 to August 2019 were collected. They were all non-manual workers and retirees from enterprises and institutions. The difference in the nutritional status of vitamin D was analyzed between different genders and among different age groups. The differences in body mass index(BMI), fasting plasma glucose(FPG), total cholesterol(TC), triglyceride(TG), high density lipoprotein(HDL) and low density lipoprotein(LDL) were compared between the vitamin D normal group and the vitamin D insufficiency/deficiency group. The correlation between serum total 25-hydroxyvitamin D［25-(OH)D］ level and age, BMI, blood glucose and blood fat was analyzed. Results　The median level of serum total 25-(OH)D was 71.68(56.37, 87.44)nmol/L in the 494 subjects, among whom 211 cases(42.71%) had normal vitamin D, and 283 cases(57.29%) had insufficient/deficient vitamin D. The serum total 25-(OH)D level in males was higher than that in females, and the proportion of those with normal nutritional status of vitamin D was higher, the difference was statistically significant(P<0.05). The median levels of serum total 25-(OH)D were 64.17(50.85, 80.29)nmol/L, 73.58(57.15, 85.89)nmol/L and 77.83(63.45, 98.02)nmol/L, and the normal rates of nutritional status of vitamin D were 31.44%, 46.67% and 54.07% in the 18～45 years old group, the 46～60 years old group and the >60 years old group, respectively, and the differences were statistically significant(P<0.05). There were no significant differences in BMI, FPG, TC, TG, HDL and LDL between the vitamin D normal group and the vitamin D insufficiency/deficiency group(P>0.05). The serum total 25-(OH)D level was positively correlated with age(r=0.266, P=0.000), but it was not significantly correlated with BMI, FPG, TC, TG, HDL and LDL(P>0.05). Conclusion　The detection rate of vitamin D insufficiency/deficiency is high in non-manual workers and retirees. It is recommended that non-manual workers, especially indoor office workers and young females, take active measures to take vitamin D supplements.]]> 2021/12/3 11:58:04 PANG Yu, HUANG Guo-xiu, WU Chao-chun, et al. 552true ［Abstract］　Objective　To analyze the clinical characteristics of abdominal wall endometriosis(AWE) and to provide reference for the diagnosis and treatment of AWE. Methods　The clinical data and the follow-up data of 47 AWE patients admitted to Guiyang Maternity and Child Health Care Hospital from January 2015 to December 2020 were collected, and their characteristics were analysed. Results　The average age of the patients was (32.17±4.45)years at the time of admission, and they all had a history of cesarean section. Among them, 32 cases(68.09%) received one cesarean section and 15 cases(31.91%) received two cesarean sections. There were 43 cases(91.49%) with abdominal transverse incision and 4 cases(8.51%) with abdominal longitudinal incision. The time from the patients′ first onset to the last cesarean section was 2-120 months. Thirty-seven cases(78.72%) had abdominal mass pain during menstruation as the first symptom. The location of the masses was more common on the left and right sides of the transverse incision in cesarean section. All the 47 patients with AWE underwent abdominal wall ultrasound examination, forty-two of whom(89.36%) showed irregular hypoechoic masses at the incision of the abdominal wall, with unclear borders, and blood flow signals in some masses. Among the 47 AWE patients, 29 cases underwent CA125 detection, with an average value of (23.52±12.81)U/ml. All the 47 patients underwent surgical resection of the lesions, and the average diameter of the lesions was (2.55±0.85)cm. Most of the lesions involved the fascia and muscle layers. Six patients relapsed during the postoperative follow-up. Conclusion　AWE has typical clinical manifestations after cesarean section, which can be diagnosed in combination with medical history and B-ultrasound results. Surgical treatment is the first choice for AWE, and complete removal of the lesions during the operation is the key to the treatment of this disease. Clinicians should prevent the occurrence of iatrogenic AWE during abdominal surgery, and formulate a standardized management model and follow up the patients closely after surgery.]]> 2021/11/2 15:11:32 MA Lan-fang, CAO Li-li, WU Zhang-ying, et al. 551true ［Abstract］　Objective　To explore the clinical value of using three-dimensional(3D) printed lumbar nerve root anomalies(LNRA) model to measure the change of Kambin triangle area. Methods　Four LNRA patients, aged 44-65 years, admitted to Guigang City People′s Hospital from July 2018 to June 2020, were selected, including 2 males and 2 females. Variation types: 3 cases of root-associated type, 1 case of caudal origin type. Digital medical modeling is performed by collecting 3D magnetic resonance imaging(MRI) data of the patients′ waist, and the relevant parameters of Kambin triangle area were measured. The patients′ LNRA model was constructed through 3D printing technology, and the simulation operation of placing tube with aperture mirror was implemented on the model. Results　The simulation operation based on the 3D printing model found that the Kambin triangle areas of the intervertebral foramen of the 4 patients were 0.00 mm², 11.60 mm², 15.33 mm² and 22.20 mm², respectively, and the maximum permissible cannula diameters were 0.00 mm, 2.74 mm, 3.88 mm and 3.93 mm, respectively; the Kambin triangle areas in the spinal canal were 0.00 mm², 26.72 mm², 36.38 mm² and 76.88 mm², respectively, and the maximum permissible cannula diameters were 0.00 mm, 3.29 mm, 5.33 mm and 7.18 mm, respectively. Conclusion　The 3D printed LNRA model allows clinicians to have an intuitive and three-dimensional understanding of the changes in the Kambin triangle area of LNRA patients, which is beneficial to reducing surgical risks and improving curative effects.]]> 2021/11/2 15:11:32 ZHANG Qi-biao, JIANG Jian-zhong, LUO Xiang, et al. 550true ［Abstract］　Objective　To explore the relationship between serum lncRNA-ENST00000414355 and essential hypertension. Methods　Three hundred and sixty patients with essential hypertension(the observation group) who were admitted to the Second People′s Hospital of Futian District Shenzhen City from July 2019 to November 2019 were selected. According to the 1∶1 matching principle, three hundred and sixty healthy subjects receiving physical examination at the Second People′s Hospital of Futian District Shenzhen City during the same period were selected as the control group. Real-time quantitative reverse transcription polymerase chain reaction(RT-qPCR) was used to detect the expression level of lncRNA-ENST00000414355 in the subjects′ serum. The clinical data were compared between the two groups, and the relationship between the level of lncRNA-ENST00000414355 and essential hypertension was analyzed. Results　There were no significant differences in alcohol consumption, regular exercise, history of diabetes, and fasting blood glucose, triglyceride(TG) and high-density lipoprotein(HDL) levels between the two groups(P>0.05). The proportion of smokers, and the levels of Body Mass Index(BMI), total cholesterol(TC), low-density lipoprotein(LDL), and lncRNA-ENST00000414355 in the observation group were higher than those in the control group, and the differences were statistically significant(P<0.05).The results of logistic regression analysis showed that smoking behavior(OR=2.231), higher levels of BMI(OR=1.994), TC(OR=1.278), and lncRNA-ENST00000414355(OR=2.057) were the risk factors promoting the occurrence of essential hypertension(P<0.05). The results of Pearson correlation analysis showed that the level of serum lncRNA-ENST00000414355 was positively correlated with systolic blood pressure, diastolic blood pressure, BMI, TC and LDL(P<0.05). Conclusion　Serum lncRNA-ENST00000414355 is highly expressed in patients with essential hypertension, and is positively related to blood pressure and blood lipids. lncRNA-ENST00000414355 has an application prospect as a marker for essential hypertension.]]> 2021/11/2 15:11:32 ZHOU Zhi-heng, HUANG Zhi-jie, CHEN Bao-xin, et al. 549true ［Abstract］　Objective　To explore the clinical efficacy of Shengqi Jiangtang Prescription combined with metformin sustained-release tablets in treatment of type 2 diabetes mellitus with qi-yin deficiency. Methods　 Eighty patients suffering from type 2 diabetes mellitus with qi-yin deficiency who were admitted to the Department of Endocrinology, Baiyin Hospital of Integrated Traditional Chinese and Western Medicine from December 2019 to November 2020 were selected, and the patients were divided into study group and control group in the order of their treatments, with 40 cases in each group. The control group was treated with metformin sustained-release tablets, and the study group was treated with Shengqi Jiangtang Prescription plus the same treatment as the control group. The course of treatment was 4 weeks in both groups. The levels of fasting plasma glucose(FPG), plasma glucose of 2 hours post glucose-load(2hPG), glycated hemoglobin(HbA1c), low-density lipoprotein cholesterol(LDL-C), homocysteine(Hcy) as well as Body Mass Index(BMI) and Traditional Chinese Medicine(TCM) syndrome scores were compared between the two groups before and after treatment. The clinical efficacy and the incidence of adverse reactions were observed in the two groups. Results　After treatment, the levels of FPG, 2hPG, HbA1c, LDL-C, Hcy, BMI and TCM syndrome scores were significantly lower than those before treatment in the two groups(P<0.05), and the post-treatment levels of the study group were significantly lower than those of the control group(P<0.05). The total effective rate of the study group was significantly higher than that of the control group(87.50% vs 50.00%; χ²=10.751, P=0.001), and there was no significant difference in the incidence of adverse reactions between the two groups(P>0.05). Conclusion　Compared with metformin sustained-release tablets alone, Shengqi Jiangtang Prescription combined with metformin sustained-release tablets has better efficacy and safety in the treatment of type 2 diabetes mellitus with qi-yin deficiency. The combination therapy is worthy of clinical promotion.]]> 2021/11/2 15:11:32 BAI Hong-xia, GAO Yun-feng, YANG Rui-juan, et al. 548true ［Abstract］　Objective　To explore the effect and mechanism of berberine(Ber) in reversing the resistance of human colon cancer cells to oxaliplatin(OXA). Methods　Human colon cancer OXA-resistant cells THC-8307/OXA were selected for experiment, and they were divided into control group, OXA group and Ber(5, 10, 20 μg/ml)+OXA groups. Cell morphology was observed by inverted phase contrast microscope. Cell survival rate was detected by methyl thiazolyl tetrazolium(MTT) assay method, and cell apoptosis rate was detected by flow cytometry. Multidrug resistanc-1(MDR-1) mRNA expression was detected by real-time quantitative reverse transcription-polymerase chain reaction(RT-qPCR), and the expressions of P-glycoprotein(P-gp), phosphatidylinositol 3-kinase(PI3K), protein kinase B(Akt) and phosphorylated protein kinase B(p-Akt) proteins were detected by Western blot. Results　Compared with those of the OXA group, the cell survival rates of Ber(5, 10, 20 μg/ml)+OXA groups were significantly reduced(P<0.05), and the apoptosis rates were significantly increased(P<0.05); the expression levels of MDR-1 mRNA, P-gp protein, p-Akt protein, PI3K protein, and p-Akt/Akt value were significantly reduced(P<0.05). Among them, the effects of Ber(10, 20 μg/ml)+OXA groups were significantly higher than those of Ber(5 μg/ml)+OXA group, and the effect of Ber(20 μg/ml)+OXA group was significantly higher than that of Ber(10 μg/ml)+OXA group. There were no significant differences in the detection results between the OXA group and the control group(P>0.05). Conclusion　Ber can significantly reverse the resistance of THC-8307/OXA cells to OXA, and its mechanism of action may be related to the inhibition of PI3K/Akt signaling pathway and the down-regulation of P-gp protein expression.]]> 2021/11/2 15:11:32 CHEN Lin, LI Wei, HUANG Guo-xiu, et al. 547true ［Abstract］　Objective　To analyze the clinical feature of fulminant type 1 diabetes mellitus(FT1DM), and to improve clinicians′ understanding of FT1DM. Methods　The clinical data of 15 patients with FT1DM admitted to the Ninth Affiliated Hospital of Guangxi Medical University from October 2003 to December 2019 were collected, and the clinical data of 49 patients with non-fulminant type 1 diabetes mellitus(NFT1DM) during the same period were selected for analysis. The NFT1DM patients were divided into short-course NFT1DM group(25 cases) and long-course NFT1DM group(24 cases) according to the course of the disease, and were divided into low C-peptide NFT1DM group(27 cases) and high C-peptide NFT1DM group(22 cases) according to the levels of postprandial C-peptide(PCP), and were divided into low glycated hemoglobin A1c(HbA1c) NFT1DM group(7 cases) and high HbA1c NFT1DM group(42 cases) according to the levels of HbA1c. The clinical indicators were compared among the groups. Results　Compared with the NFT1DM group, the FT1DM group was older, and had a shorter course of the disease, lower levels of HbA1c, fasting plasma C-peptide(FCP) and PCP, and higher levels of blood sugar, serum creatinine(Scr), blood urea nitrogen(BUN) and blood potassium, and the differences were statistically significant between the two groups(P<0.05). There were statistically significant differences in age, HbA1c, FCP, PCP, Scr, BUN and serum potassium among the FT1DM group, the short-course NFT1DM group and the long-course NFT1DM group(P<0.05). There were statistically significant differences in the course of the disease, blood sugar, HbA1c, FCP, Scr, BUN and serum potassium among the FT1DM group, the low C-peptide NFT1DM group and the high C-peptide NFT1DM group(P<0.05). There were statistically significant differences in age, the course of the disease, FCP, PCP, Scr, BUN and serum potassium among the FT1DM group, the low HbA1c NFT1DM group and the high HbA1c NFT1DM group(P<0.05). Conclusion　Compared with NFT1DM, FT1DM has its characteristics. The diagnostic classification of FT1DM deserves further discussion.]]> 2021/11/2 15:11:32 ZHOU Shao-bi, QIU Jun-lin 546true ［Abstract］　Objective　To compare the effects of laparoscopic ovarian cystectomy and open removal of ovarian cysts on postoperative rehabilitation and ovarian function of patients with ovarian cysts. Methods　Eighty-four patients with ovarian cysts admitted to Lianjiang Maternal and Child Health Hospital from February 2018 to February 2020 were selected and divided into observation group and control group by random number table method, with 42 cases in each group. The control group was treated with laparotomy, and the observation group was treated with laparoscopic ovarian cystectomy. The clinical indicators, ovarian function indicators［estradiol(E₂), follicle stimulating hormone(FSH), anti-Müllerian hormone(AMH)］ and prognostic indicators(relapse rate and pregnancy rate) were compared between the two groups. Results　The intraoperative blood loss of the observation group was less than that of the control group, and the operation time, the time of ambulation, and hospitalization time was shorter than that of the control group, and the differences were statistically significant(P<0.05). After surgery, the levels of E₂ and AMH in the observation group were higher than those in the control group, and the level of FSH in the observation group was lower than that in the control group, and the differences were statistically significant(P<0.05). The postoperative recurrence rate of the observation group was significantly lower than that of the control group(4.76% vs 19.05%, P<0.05), and the pregnancy rate of the observation group was significantly higher than that of the control group(71.43% vs 45.24%, P<0.05). Conclusion　Laparoscopic ovarian cystectomy is less invasive than laparotomy for the treatment of ovarian cysts and can meet the needs of the patients for early recovery after surgery, reduce the impact on ovarian function, reduce the recurrence rate of the disease, and increase the pregnancy rate.]]> 2021/11/2 15:11:32 LIN Mei 545true ［Abstract］　Objective　To explore the expressions of tumor necrosis factor receptor-associated factor 6(TRAF6) and deubiquitinating enzyme ovarian tumor domain-containing protein 5(OTUD5) in colorectal cancer(CRC), and their roles in the development and progression of colorectal cancer. Methods　The clinicopathological data of 50 CRC patients who underwent surgical treatment in the First Affiliated Hospital of Guangxi Medical University from March 2017 to March 2018 were selected. The CRC tissues and their matched paracancerous tissues(>10 cm from the tumor edge) were collected, and the expressions of TRAF6 and OTUD5 were detected by immunohistochemical staining, and their correlations with the clinicopathological characteristics of the patients were analyzed. The expression levels of TRAF6 mRNA and OTUD5 mRNA in human normal colon epithelial cell line NCM460 and CRC cell line HT29 were detected by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR) method. The HT29 cell line overexpressing TRAF6 was constructed, and the correlation between TRAF6 and OTUD5 was analyzed. Results　The positive expression rate of TRAF6 in the CRC tissues was significantly higher than that in the matched paracancerous tissues(84.00% vs 28.00%; χ²=31.818, P=0.000), and the positive expression rate of OTUD5 in the CRC tissues was significantly lower than that in the matched paracancerous tissues(32.00% vs 54.00%; χ²=4.937, P=0.026). The incidence of distant metastasis in the TRAF6-negative group was significantly higher than that in the TRAF6-positive group(P<0.05). The OTUD5-negative group had a greater proportion of the patients with TNM stage Ⅲ～Ⅳ than the OTUD5-positive group, and the incidence rates of lymph node metastasis and distant metastasis in the OTUD5-negative group were higher than those in the OTUD5-positive group, and the differences were statistically significant(P<0.05). The expression level of TRAF6 mRNA in HT29 cells was significantly higher than that in NCM460 cells(t=6.960, P=0.000), and the expression level of OTUD5 mRNA in HT29 cells was significantly lower than that in NCM460 cells(t=13.840, P=0.000). In the HT29 cells overexpressing TRAF6, the expression level of OTUD5 mRNA was significantly lower than that in the normal HT29 cells(t=32.555, P=0.000). Conclusion　TRAF6 may be involved in the occurrence, development and metastasis of CRC by inhibiting the expression of OTUD5 gene.]]> 2022/9/30 11:37:10 CHEN Da, WU Jiang-ni, WEI Er-dan, et al. 544true ［Abstract］　Objective　To analyze the clinical characteristics and outcomes of patients with chronic myeloid leukemia in chronic phase(CML-CP) after discontinuation of tyrosine kinase inhibitor(TKI) therapy. Methods　The clinical data of 8 CML-CP patients treated in Department of Hematology, the Fourth Affiliated Hospital of Zhejiang University School of Medicine, from March 2015 to August 2021 were retrospectively analyzed. All the patients were discontinued after reaching the criteria for drug discontinuation and were followed up regularly. The patients included 4 males and 4 females, and their age ranged from 31 to 69 years, with a median age of 49 years. Three cases received first-line first-generation TKI(original drug) treatment, and 4 cases received first-line first-generation TKI(generic drug) treatment, and 1 case received first-line second-generation TKI(nilotinib) treatment. The treatment-free remission(TFR) and recurrence were observed after drug discontinuation. Results　The duration from TKI treatment to reaching MR^4.0 was 2-64 months in the 8 patients, with a median duration of 19.5 months. The duration of maintaining MR^4.0 and above was 24-144 months, with a median duration of 60 months. The overall duration of TKI treatment was 48 to 146 months, with a median duration of 95 months. All the 8 patients were discontinued after meeting the criteria of relevant guidelines. By August 2021, the duration of drug discontinuation in the 8 patients ranged from 2 to 64 months, with a median duration of 11.5 months. Four patients had no recurrence, and the overall TFR rate was 50.00%. Among the non-recurrent cases, 1 case received first-line second-generation TKI treatment, and 2 cases received first-line first-generation TKI(original drug), and 1 case received first-line first-generation TKI(generic drug). Among the recurrent patients, 2 patients had recurrence within 6 months, and 2 patients were discontinued for more than 6 months. After recurrence, all the recurrent patients took medication again and achieved MR^4.0. Among them, 3 patients turned negative within 3 months after taking the first-generation TKI(generic drug), and 1 patient turned negative within 15 months after switching to the second-generation TKI treatment. Conclusion　In this study, the total TFR rate of CML-CP patients after drug discontinuation is 50.00%, and the TFR rates of patients receiving first-line second-generation TKI and first-line first-generation TKI(original drug) are better.]]> 2022/9/30 11:37:10 JIN Ke-yi, ZHANG Lan, WANG Sheng-jie, et al. 543true ［Abstract］　Objective　To analyze the clinical characteristics of prostate cancer patients in Xinjiang Uyghur Autonomous Region from 2010 to 2021, and to provide reference for the formulation of prevention and control strategies for prostate cancer in Xinjiang Uyghur Autonomous Region. Methods　The clinical data of 2 151 patients with prostate cancer who were admitted to the First Affiliated Hospital of Xinjiang Medical University, the People′s Hospital of Xinjiang Uyghur Autonomous Region, the Cancer Hospital Affiliated to Xinjiang Medical University, and Xinjiang Production and Construction Corps Hospital from January 2010 to June 2021 were collected. The clinical characteristics of the prostate cancer patients were analyzed. Results　The patients in this group were 41-96(71.81±8.20)years old, and most of them were 70-79 years old(1 030 cases, 47.89%). The majority of nationalities were Han nationality(1 477 cases, 68.67%), followed by Uyghur nationality(500 cases, 23.25%). From 2010 to 2021, the number of cases showed an increasing trend. Nine hundred and forty-one cases(43.75%) had dysuria at the time of initial diagnosis. The median prostate-specific antigen(PSA) detection value of the patients at the time of initial diagnosis was 54.68 ng/ml. Two hundred and ninety-nine cases(13.90%) had the PSA detection value ≤10.0 ng/ml, and 337 cases(15.67%) had the PSA detection value in between 10.1 ng/ml and 20.0 ng/ml, and 1 515 cases(70.43%) had the PSA detection value >20.0 ng/ml. Most patients had a Gleason score of 8 points(593 cases, 27.57%). Among the 2 151 patients, 1 274 patients(59.23%) had early-stage prostate cancer, and 877 patients(40.77%) had distant metastasis, mainly bone metastasis, and 438 patients(20.36%) received radical prostatectomy for prostate cancer. Compared with Han patients, Uyghur patients were diagnosed with prostate cancer at a younger age, and had larger prostate volume, higher PSA level, and a lower proportion of patients receiving radical prostatectomy for prostate cancer, and the differences were statistically significant(P<0.05). The proportion of Han patients with increased PSA as the first clinical manifestation during physical examination was greater than that of Uyghur patients, and the proportion of Uyghur patients whose first clinical manifestations were urinary retention and dysuria was greater than that of Han patients, and the differences were statistically significant(P<0.05). The proportion of localized tumor type in Han patients was higher than that in Uyghur patients, while the proportion of distant metastatic tumor type was higher in Uyghur patients, and the differences were statistically significant(P<0.05). For those patients with distant metastases, the incidence of visceral metastases and the proportion of patients receiving systemic therapy in Han patients were higher than those in Uyghur patients, while the incidence of bone metastases was higher in Uyghur patients, most of whom received endocrine therapy alone, and the differences were statistically significant(P<0.05). Conclusion　The disease spectrum of prostate cancer patients in Xinjiang Uyghur Autonomous Region is unique, and analyzing its differential characteristics can provide supporting evidence for the local formulation of disease prevention and control strategies.]]> 2022/9/30 11:37:10 WANG Wen-guang, WANG Chen-yu, BI Xing, et al. 542true ［Abstract］　Objective　To explore the application value of computed tomography(CT) enterography(CTE) in differentiating active staging of Crohn′s disease(CD). Methods　The clinical data of 60 CD patients admitted to the Affiliated Hospital of Yangzhou University from June 2019 to December 2021 were selected. According to their different Crohn′s disease activity index(CDAI) scores, the patients were divided into group A(with CDAI scores ≤220 points, during remission and mild activity period, 31 cases) and group B(with CDAI scores ≥221 points, during moderate and severe activity period, 29 cases). Binary logistic regression was used to analyze the parameters associated with the stage of CD activity. Results　The intestinal wall thickness, the CT value of the intestinal wall in the arterial phase, and the CT value of the intestinal wall in the venous phase in the group B were greater than those in the group A, and the proportion of the intestinal wall stratified enhancement, the comb-tooth sign, and the increased mesangial fat density in the group B were higher than those in the group A, and the differences were statistically significant(P<0.05). The results of binary logistic regression analysis showed that intestinal wall thickness［OR(95%CI): 3.470(1.178-10.223)］, the CT value of the intestinal wall in the venous phase［OR(95%CI): 1.289(1.067-1.557)］ and intestinal wall stratification enhancement［OR(95%CI): 6.784(1.027-44.759)］ were significantly associated with the active stage of CD. Conclusion　Intestinal wall thickness, intestinal wall stratification enhancement, and CT value of the intestinal wall in the venous phase of CTE can help to identify the active stage of CD.]]> 2022/9/30 11:37:10 HE Jiang-tao, XUE Zhen-long, WANG Wei, et al. 541true ［Abstract］　Objective　To explore the operation skills of natural orifice specimen extraction surgery(NOSES) for transanal eversion resection of low rectal cancer under laparoscope. Methods　A total of 67 patients with low rectal cancer who underwent NOSES for transanal eversion resection of low rectal cancer under laparoscope in Sichuan Cancer Hospital from January 2017 to December 2021 were selected. The operation time, intraoperative blood loss, postoperative complications, hospital stay, tumor recurrence and metastasis of the patients were analyzed. Results　All the 67 patients in this group were successfully operated, and none of them was converted to laparotomy. Among them, 3 patients underwent unilateral lateral lymph node dissection at the same time, and 43 patients(64.2%) underwent prophylactic ileostomy during the operation. The operation time ranged from 200 to 590 minutes, with an average of 320.1 minutes. Intraoperative blood loss was 5 to 300 ml, with an average of 64.3 ml. No case of abdominal contamination occurred. After surgery, the average time to exhaust gas through the anus or stoma was 21.9 hours(1-88 hours). Postoperative anastomotic leakage occurred in 1 case, urinary retention in 3 cases, and incomplete intestinal obstruction in 5 cases, and all the patients with these complications were cured by conservative treatments. The average postoperative hospital stay was 10.6 days(7-26 days). After surgery, the patients were followed up until June 2022, and pelvic recurrence was found in 1 case and distant metastasis in 5 cases. Conclusion　NOSES for transanal eversion resection of low rectal cancer under laparoscope has the advantages of less trauma, quick recovery and good curative effect. As long as the indications are strictly controlled before surgery, and the aseptic and tumor-free principles are strictly observed, and the key operating skills are mastered, the abdominal cavity contamination can be effectively avoided and the risk of iatrogenic tumor spread can be reduced.]]> 2022/9/30 11:37:10 ZHAO Xiao-fang, FU Chuan-chuan, ZHENG Yang-chun, et al. 540true ［Abstract］　Objective　To investigate the effects of early enteral nutrition with different routes on the prognosis of patients after acute Stanford type A aortic dissection(ATTAD). Methods　Seventy-two patients after ATTAD who were admitted to the Intensive Care Unit(ICU) of Henan Provincial Chest Hospital from August 2020 to December 2021 were selected and divided into an observation group and a control group by random number table method, with 36 cases in each group. The observation group received enteral nutrition therapy with naso-intestinal tube, and the control group received enteral nutrition therapy with nasogastric tube. The differences in feeding tolerance, nutritional status and clinical prognosis were compared between the two groups. Results　There was no significant difference in the indwelling time of the nasogastric tube between the observation group and the control group［(6.94±0.89)d vs (7.33±1.21)d; t=1.544, P=0.127］. During the treatment period, the incidence rates of abdominal distension, reflux and gastric retention in the control group were significantly higher than those in the observation group(P<0.05). Compared with those in the control group, the mechanical ventilation time, the duration of ICU stay and the length of total hospital stay in the observation group were shorter, and the differences were statistically significant(P<0.05). There were no significant differences in the incidence rates of aspiration pneumonia and incision infection between the two groups(P>0.05). On the first day, the third day and the fifth day after receiving nutritional treatment, the levels of albumin(ALB) and prealbumin(PAB) in the two groups showed an upward trend, and the increasing range of the observation group was greater, and the differences were statistically significant(P<0.05). Conclusion　Early enteral nutrition support via naso-intestinal tube can effectively improve the feeding tolerance and nutritional status of patients after ATTAD, which is beneficial to the recovery of the patients.]]> 2022/9/30 11:37:10 CHE Li-ling, LIU Ya-qian, HOU Jian-yong, et al. 539true ［Abstract］　Objective　To study the application value of ultrasonic pressure-strain loop(PSL) technique in evaluating left ventricular myocardial work in patients with connective tissue disease complicated with interstitial lung disease(CTD-ILD). Methods　Twenty-eight CTD-ILD patients who were admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region from February 2018 to December 2019(CTD-ILD group) and twenty-eight gender and age-matched healthy individuals receiving health checkup were recruited as the control group during the same period. Blood pressure measurements and conventional echocardiography were completed for all the selected subjects. The relevant dynamic images were collected and analyzed by using EchoPAC software and the myocardial work parameters of the left ventricles were compared between the two groups, including global work index(GWI), global constructive work(GCW), global wasted work(GWW) and global work efficiency(GWE). The correlation between the parameters of myocardial work and left ventricular strain was analyzed. Results　Compared with those in the control group, GWW in the CTD-ILD group increased(P<0.05), and the absolute value of global longitudinal strain(GLS), GWI, GCW and GWE decreased, and the differences were significant(P<0.05). GWI and GWE were positively correlated with the absolute value of GLS(P<0.05), and GWW was negatively correlated with the absolute value of GLS(P<0.05). Conclusion　PSL can quantitatively evaluate the left ventricular myocardial work status in patients with CTD-ILD, which provides more reference information for clinical practice.]]> 2022/9/30 11:37:10 SHEN Jie, ZHENG Hong-yu, ZHAO Yi-lan, et al. 538true ［Abstract］　Objective　To explore the clinical efficacy of abdominal needle combined with routine swallowing rehabilitation training in treatment of post-stroke swallowing disorder. Methods　Sixty patients with post-stroke swallowing disorder who were admitted to Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine from September 2020 to December 2021 were selected and divided into an observation group and a control group by random number table method, with 30 cases in each group. The control group received routine swallowing rehabilitation training, and the observation group received abdominal needle treatment based on the treatment plan of the control group, and the course of treatment in both groups was 4 weeks. The videofluoroscopic swallowing study(VFSS) score, mobility of the hyoid laryngeal complex, pharyngeal constriction ratio(PCR), and opening degree of upper esophageal sphincter(UES) and clinical efficacy were compared between the two groups before and after treatment. Results　The VFSS scores in the two groups were improved after treatment compared with those before treatment, and the VFSS scores of the observation group were higher than those of the control group, and the differences were statistically significant(P<0.05). The movement ranges of hyoid laryngeal complex(upward movement and forward movement of hyoid bone, and upward movement and forward movement of thyroid cartilage) after treatment in both groups were increased compared with those before treatment(P<0.05), and the changes in the observation group were more significant, and the differences were statistically significant(P<0.05). After treatment, the opening degrees of UES in the two groups were increased compared with those before treatment, and the levels of PCR in the two groups were decreased compared with those before treatment, and the changes in the observation group were more significant than those in the control group, and the differences were statistically significant(P<0.05). The total effective rate of the observation group was higher than that of the control group, and the difference was statistically significant(96.67% vs 70.00%; χ²=7.680, P=0.006). Conclusion　Abdominal needle combined with routine swallowing rehabilitation training can effectively treat post-stroke swallowing disorder and promote the recovery of the patients′ swallowing function.]]> 2022/9/30 11:37:10 JIN Xin, SHAO Tian-xiang, LIU Hai-lan, et al. 537true ［Abstract］　Objective　To analyze the occurrence and influencing factors of metabolic syndrome(MS) in urban and rural residents in Guangxi, and to provide scientific basis for the formulation of prevention and control strategies on MS. Methods　Multi-stage cluster random sampling method was used. From August 2015 to November 2015, 10 counties(districts) in Guangxi, including Guiping City, Binyang County, Hepu County, Lingyun County, Fuchuan County, Luocheng County, Xingning District of Nanning City, Liubei District of Liuzhou City, Qinbei District of Qinzhou City, and Xiufeng District of Guilin City were selected as the survey points. Three townships(subdistricts) were selected from each monitoring point, and 2 administrative villages(neighborhood committees) were selected from each township(subdistrict), and 20 households were selected from each administrative village(neighborhood committee) for investigation. This study finally included 2 469 respondents. Questionnaire surveys, diet and dietary surveys, anthropometric measurements and blood biochemical tests were carried out. Results　In this survey, 574 patients were diagnosed with MS and 1 895 patients were diagnosed with non-MS, and the prevalence of MS was 23.25%. The prevalence of MS in the urban residents was 29.18%(213/730), and the prevalence of MS in the township residents was 20.76%(361/1 739), with a statistically significant difference(χ²=20.424, P=0.000). The incidence rates of hypertension and elevated triglyceride(TG) in the male MS group were higher than those in the female MS group, and the incidence rates of central obesity and reduced high-density lipoprotein cholesterol(HDL-C) in the male MS group were lower than those in the female MS group, and the differences were statistically significant(P<0.05). For the urban residents, the daily intake of cereals in the MS group was lower than that in the non-MS group, and the difference was statistically significant(P<0.05). There were no statistically significant differences in the daily intakes of fresh vegetable, fresh fruit, animal food, soybeans and nuts, edible oil and spices and salt(P>0.05). For the township residents, there were no significant differences in the daily intakes of various food types between the MS group and the non-MS group(P>0.05). The results of binary logistic regression analysis showed that female and family history of diabetes were the risk factors for the occurrence of MS(P<0.05); the age of 18-24 years taken as reference, age ≥45 years was a risk factor for the occurrence of MS(P<0.05); normal body weight taken as reference, weight loss was a protective factor against the occurrence of MS(P<0.05), while overweight and obesity were risk factors for the occurrence of MS(P<0.05); light physical labor taken as reference, medium and heavy physical labors were a protective factors against the occurrence of MS(P<0.05). Conclusion　The occurrence of MS in urban and rural residents in Guangxi is related to gender, age, family history of diabetes, body weight, labor intensity and dietary factors. When prevention and control strategies are formulated, attention should be paid to the screening of high-risk groups. Health education should be used to make urban and rural residents develop good life behaviors and habits to reduce the prevalence of MS.]]> 2022/9/30 11:37:10 LU Wu-tao, ZHOU Wei-wen, DONG Mo, et al. 536true ［Abstract］　Objective　To investigate the efficiency of bilateral uterine artery blood flow parameters and early diastolic notch in assessing adverse pregnancy outcomes in pregnant women with recurrent miscarriage in early pregnancy. Methods　Forty cases of recurrent miscarriage admitted to Putian Maternal and Child Health Hospital from August 2020 to August 2021 were selected as the observation group, and 40 cases of normal pregnancy during the same period were selected as the control group. All the patients were examined with Voluson 730 Pro color Doppler ultrasound diagnostic instrument to obtain bilateral uterine artery blood flow parameters［bilateral uterine artery peak systolic velocity/end diastolic velocity(S/D) value, uterine artery pulse index(PI) value and uterine artery resistance index(RI) value］, and the occurrence of early diastolic notch was observed. The levels of bilateral uterine artery blood flow parameters and the incidence of early diastolic notch were compared between the two groups. The correlation between early diastolic notch and adverse pregnancy outcomes was analyzed. The receiver operator characteristic(ROC) curve method was used to assess the efficiency of bilateral uterine artery blood flow parameters in predicting adverse pregnancy outcomes in pregnant women with recurrent miscarriage. Results　The S/D value, PI value and RI value of the observation group were higher than those of the control group, and the differences were statistically significant(P<0.05). The proportion of the patients with early diastolic notch in the observation group was larger than that in the control group(90.00% vs 10.00%; χ²=48.050, P=0.000). The incidence rates of gestational hypertension, gestational diabetes mellitus, eclampsia and preterm birth in the patients with early diastolic notch were significantly higher than those without early diastolic notch, and the differences were statistically significant(P<0.05). The results of ROC curve analysis showed that the S/D value, PI value and RI value all had the predictive value of adverse pregnancy outcomes in pregnant women with recurrent miscarriage(P<0.05), and the PI value had the highest efficiency［AUC(95%CI)=0.697(0.570-0.824), P=0.013］. Conclusion　The detection of bilateral uterine artery blood flow parameters and early diastolic notch in pregnant women with recurrent miscarriage can assess the occurrence of adverse pregnancy outcomes and help to guide clinical treatment.]]> 2022/9/30 11:37:10 ZHANG Ying 535true ［Abstract］　Objective　To explore the application effect of the new improved buffy coat method in the preparation of platelet concentrates(PC). Methods　Two hundred and twenty bags of 400 ml whole blood and 30 bags of 300 ml whole blood collected at Zhuzhou Central Blood Station from May 2019 to March 2020 were randomly selected. The effects of different centrifugation procedures and different whole blood volumes on PC quality were compared to determine the preparation procedure of the new improved buffy coat method. The quality of PC prepared by the enriched plasma method, the improved buffy coat method and the new improved buffy coat method was compared. Results　Whole blood centrifugation scheme 6(whole blood centrifugation: relative centrifugal force, 1 993×g; buffy coat centrifugation: relative centrifugal force, 163×g) had the highest platelet recovery rate. There were no significant differences in the mixed amount of red blood cells and the mixed amount of white blood cells of PC prepared by different centrifugation schemes(P>0.05). The mixed amount of red blood cells of PC in the 400 ml whole blood group was significantly higher than that in the 300 ml whole blood group(P<0.05), and the mixed amount of white blood cells in the 400 ml whole blood group was slightly higher than that in the 300 ml whole blood group, but the difference was not statistically significant(P>0.05). The recovery rate of PC in the 400 ml whole blood group was higher than that in the 300 ml whole blood group, and the difference was statistically significant(P<0.05). The amount of leukocytes mixed in PC prepared by the new improved buffy coat method was lower than that in the enriched plasma method group and the improved buffy coat method group, and the platelet recovery rate of the new improved buffy coat method was the highest, with statistically significant differences among the three groups(P<0.05). Conclusion　The new improved buffy coat method to prepare PC has a higher platelet recovery rate, and less mixed amount of red blood cells and leukocytes, which is worthy of wide application.]]> 2022/9/1 11:53:33 XIONG Zhi-gao, LIU Xin-chen, YANG Xiao-luo, et al. 534true ［Abstract］　Objective　To investigate the effects of short-interval second ejaculation on semen quality and spermatozoon dynamic indicators. Methods　Fifty-nine couples who received in vitro fertilization at the Reproductive Medicine and Genetic Center of the People′s Hospital of Guangxi Zhuang Autonomous Region from May 2016 to June 2020 were selected. The females′ infertility factors were excluded, and the first ejaculation and the short-interval second ejaculation(within 2 hours of the first ejaculation) were completed in all the males. The differences in the semen quality and spermatozoon dynamic indicators of the two semen collections were analyzed. Results　The semen volume, the immotile sperm concentration, and the percentage of immotile sperms in the short-interval second ejaculation were less than those in the first ejaculation, while the concentration of forward motile sperms, the percentage of forward motile sperms, and the percentage of non-forward motile sperms in the short-interval second ejaculation were greater than those in the first ejaculation, and the differences were statistically significant(P<0.05). There were no significant differences in semen concentration, round cell concentration and non-forward motile sperm concentration between the two ejaculations(P>0.05). The curvilinear motion rate, the linear motion rate, the average path velocity, the whiplash frequency and the forwardness proportion in the short-interval second ejaculation were all greater than those in the first ejaculation, and the differences were statistically significant(P<0.05). Conclusion　Short-interval second ejaculation can significantly improve semen quality and spermatozoon dynamic indicators, which has a certain clinical application value.]]> 2022/9/1 11:53:33 CHEN Liang-shi, HUANG Yue-yue, ZHANG Xiao-hui, et al. 533true ［Abstract］　Objective　To analyze the clinical data of 13 patients with low-grade appendiceal mucinous neoplasm misdiagnosed as ovarian tumor and to improve clinicians′ understanding of the disease to reduce misdiagnosis and missed diagnosis. Methods　The clinical data of 13 female patients with low-grade appendiceal mucinous neoplasm admitted to the Affiliated Changzhou NO.2 People′s Hospital of Nanjing Medical University from January 2010 to December 2019 were selected, all of whom were misdiagnosed with ovarian tumor. The patients′ clinical manifestations, auxiliary examination results, treatment methods and prognosis were analyzed. Results　The clinical manifestations of the 13 patients with low-grade appendiceal mucinous neoplasm were atypical, among whom 8 patients presented with abdominal pain as the initial symptom, and 4 patients were found to have abdominal masses as the initial symptom during examination. Eight patients underwent preoperative ultrasonography, and the tumors were considered to be from the appendix in 3 cases, the ovary in 3 cases, and other sites in 2 cases. Preoperative computed tomography(CT) was performed on 10 patients, and the tumors were considered to be from the appendix in 4 cases, the ovary in 5 cases, and other sites in 1 case. All the 13 patients underwent preoperative detections of carcinoembryonic antigen(CEA), alpha-fetoprotein(AFP), cancer antigen(CA)125 and CA199 tumor markers. The results showed that CEA was elevated in 4 patients, and CA125 was elevated in 3 patients, and CA199 was elevated in 2 patients, and no patients had elevated APF. All the 13 patients underwent surgical treatment, among whom 7 patients underwent laparoscopic exploration and 6 patients underwent laparotomy. The postoperative pathological results confirmed that these tumors were low-grade appendiceal mucinous neoplasm. Follow-up was performed on the patients until February 2020, and 2 patients died. One patient(the patient complicated with colon cancer) is currently receiving chemotherapy, and the other 10 patients are in good condition. Conclusion　For the middle-aged and elderly female patients whose initial symptom is abdominal pain or abdominal masses, if the diagnosis of ovarian cancer is not clear, the possibility of appendiceal mucinous tumor disease should be considered. Surgical exploration should be performed on the patients in time, and if necessary surgical consultations should be asked for before or during the operation. Intra-operative rapid frozen section of pathological examination can be carried out to confirm the diagnosis. Ultrasonography, CT examination and the detections of tumor markers have certain reference significance for determining the nature of the masses.]]> 2022/9/1 11:53:33 ZHONG A-hong, CHEN Yu-qing, JIANG Peng-cheng, et al. 532true ［Abstract］　Objective　To observe the application effect of modified subcutaneous tunnel continued lumbar cerebrospinal fluid drainage on brain tumor patients. Methods　Eighty brain tumor patients with indwelling lumbar drainage tubes who were admitted to Department of Neurosurgery, Affiliated Cancer Hospital of Guangzhou Medical University from August 2014 to July 2021 were selected and randomly divided into a control group and an observation group, with 40 cases in each group. Two cases in the control group were excluded due to loss to follow-up, and 38 cases were finally included. No cases were excluded from the observation group. The control group was treated with conventional lumbar drainage, while the observation group was treated with the modified subcutaneous tunnel continued lumbar cerebrospinal fluid drainage. The incidence rates of adverse events such as cerebrospinal fluid leakage at the puncture point, catheter displacement, unplanned extubation and secondary intracranial infection were compared between the two groups, and the average daily drainage volume was compared between the two groups. Results　The incidence rates of cerebrospinal fluid leakage at the puncture point, catheter displacement and unplanned extubation in the observation group were lower than those in the control group, and the differences were statistically significant(P<0.05). There was no significant difference in the incidence of secondary intracranial infection between the two groups(P>0.05). There was no significant difference in the average daily drainage volume between the two groups［(159.07±30.20)ml vs (162.42±32.45)ml; t=0.472, P=0.638］. Conclusion　The modified subcutaneous tunnel continued lumbar cerebrospinal fluid drainage has satisfactory cerebrospinal fluid drainage effect, and can significantly reduce the risks of adverse events such as cerebrospinal fluid leakage at the puncture point, catheter displacement and unplanned extubation. The operation of this drainage is simple and easy, which is worthy of clinical application.]]> 2022/9/1 11:53:33 LI Dan, LUO Dong-dong, ZHAO Hai-lin, et al. 531true ［Abstract］　Objective　To analyze the diagnostic efficacy of fractional exhaled nitric oxide(FeNO) combined with small airway function indicators in the diagnosis of airway hyperresponsiveness(AHR) in patients with asthma. Methods　Sixty newly diagnosed asthma patients who were admitted to the Department of Respiratory and Critical Care Medicine, the First Medical Center, Chinese People′s Liberation Army General Hospital from September 2019 to March 2022 were selected. Among them, 30 patients were positive for bronchial dilation test(BDT)(BDT-positive group); 30 patients were BDT-negative and bronchial provocation test(BPT)-positive(BPT-positive group). In addition, 30 non-asthmatic patients who had asthma-related symptoms during the same period but were negative for BDT and BPT were selected as the control group. The general clinical data were compared among the three groups, as well as the level of FeNO, percentage of forced expiratory volume in the first second to the expected value(FEV₁%pred), percentage of forced expiratory volume in the first second to forced vital capacity(FEV₁/FVC%), predicted forced vital capacity(FVC%pred), percentage of 50% forced expiratory flow to the expected value(FEF50%pred), percentage of 75% forced expiratory flow to the expected value(FEF75%pred) and percentage of maximal mid-expiratory flow to the expected value(MMEF%pred). The receiver operator characteristic(ROC) curve method was used to analyze the efficacy of FeNO and small airway function indicators in diagnosing AHR. Results　The levels of FeNO in the BDT-positive group and the BPT-positive group were significantly higher than those in the control group(P<0.05), but there was no significant difference in the level of FeNO between the BDT-positive group and the BPT-positive group(P>0.05). The levels of MMEF%pred, FEF75%pred, and FEF50%pred in the BDT-positive group and the BPT-positive group were lower than those in the control group, and the incidence rates of small airway dysfunction in the BDT-positive group and the BPT-positive group were higher than those in the control group, and the differences were statistically significant(P<0.05). However, there were no significant differences in the above indicators between the BDT-positive group and the BPT-positive group(P>0.05). The results of ROC curve analysis showed that FeNO, FEF50%pred, FEF75%pred and MMEF%pred all had efficacy in diagnosing AHR(P<0.05). In addition, FeNO combined with small airway function indicators could further improve the diagnostic efficacy. Among them, FeNO+FEF50%pred had the best diagnostic efficiency［AUC(95%CI): 0.868(0.780-0.956) ］, with a sensitivity of 86.70% and a specificity of 73.30%. Conclusion　FeNO combined with small airway function indicators can effectively diagnose AHR in patients with asthma, and can be used as a supplementary tool for early diagnosis of mild asthma.]]> 2022/9/1 11:53:33 FAN Long-mei, MENG Song, ZHANG Wei, et al. 530true ［Abstract］　Objective　To analyze the clinical features, diagnosis and treatment of primary malignant tumor of the duodenum(PMTD) in 143 cases. Methods　The clinical data of 143 PMTD patients admitted to the First Affiliated Hospital of Guangxi Medical University from January 2012 to June 2019 were retrospectively analyzed, and the indicators affecting the prognosis of the patients were analyzed. Results　Among the 143 PMTD patients, there were 83 males and 60 females, and the number of the patients aged 50 to 59 years accounted for the largest proportion of the patients in the age group of onset(52 cases, 36.36%). The main site of the disease was the descending part of the duodenum(120 cases, 83.92%), followed by the bulbar part(16 cases, 11.18%). Adenocarcinoma was the main pathological type(103 cases, 72.03%). Jaundice and abdominal pain were the most common clinical manifestations. The positive detection rates of PMTD examined by endoscopic ultrasonography, endoscopic retrograde cholangiopancreatography(ERCP) and computed tomography(CT) were 98.34%, 95.83% and 91.67%, respectively. The positive rate of carbohydrate antigen 199(CA199) was 46.15%, while the positive rates of carcinoembryonic antigen(CEA), alpha fetoprotein(AFP) and carbohydrate antigen 125(CA125) were below 10.00%. Among the 134 patients who were followed up, 7 patients had local recurrence, and the median recurrence time was 28 months; 12 patients had metastasis, and 24 patients died. The 1-, 3-, and 5-year cumulative survival rates were 86.10%, 84.02%, and 82.09%, respectively. The time from the first symptom to diagnosis ≥4 months and the occurrence of lymph node metastasis were significantly correlated with the poor prognosis of PMTD patients(P<0.05). Conclusion　The clinical manifestations of PMTD are jaundice and abdominal pain. The main site of the disease is the descending part of the duodenum, and the detection rate of the PMTD examined by digestive endoscopy is high. The long time from the first symptom to the diagnosis and the occurrence of lymph node metastasis affect the prognosis of PMTD patients, which deserves the attention of clinicians.]]> 2022/9/1 11:53:33 JIANG Dan, LIANG Xian, LIANG Zhi-hai, et al. 529true ［Abstract］　Objective　To observe the clinical efficacy of nerve mobilization combined with muscle energy technique in treatment of cervical spondylotic radiculopathy(CSR). Methods　Sixty-two CSR patients admitted to Kunming Hospital of Traditional Chinese Medicine from January 2021 to November 2021 were selected and divided into control group and observation group by the random number table method, with 31 cases in each group. The control group was treated with conventional rehabilitation therapy combined with nerve mobilization, and the observation group was treated with muscle energy technique on the basis of the same treatment in the control group. Visual Analogue Scale(VAS) score, Neck Disability Index(NDI) score and clinical efficacy were compared between the two groups before and after treatment. Results　After 2 weeks of treatment, the VAS scores and NDI scores in the two groups were significantly lower than those before treatment(P<0.05), and the VAS scores and NDI scores in the observation group were lower than those in the control group, and the differences were statistically significant(P<0.05). The clinical effective rate of the observation group was higher than that of the control group, and the difference was statistically significant(93.55% vs 74.19%; χ²=4.038, P=0.044). Conclusion　Nerve mobilization combined with muscle energy technique can effectively relieve pain and improve cervical spine dysfunction for patients with CSR, and has a good clinical effect.]]> 2022/9/1 11:53:33 CHEN Hong-bo,YAO Na, SONG Yan-ping, et al. 528true ［Abstract］　Objective　To observe the long-term effect of perioperative progressive comprehensive rehabilitation intervention on the upper limb function in patients receiving radical mastectomy for breast cancer. Methods　Eighty-five female patients who underwent radical mastectomy for breast cancer in the People′s Hospital of Guangxi Zhuang Autonomous Region from March 2018 to March 2020 were selected and randomly divided into observation group(n=45) and control group(n=40). The observation group received perioperative progressive comprehensive rehabilitation intervention, while the control group received routine rehabilitation intervention. The active range of motion and Constant score of bilateral upper extremity shoulder joints were compared between the two groups before surgery, 1 month after surgery, 3 months after surgery, and 12 months after surgery. Results　The ranges of motion of shoulder flexion, abduction, extension, internal rotation and external rotation were improved to different degrees in both groups 1 month, 3 months and 12 months after surgery, and the improvements of shoulder flexion, abduction and extension in the observation group were significantly better than those in the control group(P<0.05). The incidence rates of upper extremity lymphedema in the observation group were lower than those in the control group 1 month, 3 months and 12 months after surgery, but the differences were not statistically significant(8.89% vs 12.50%, 13.33% vs 22.50%, 15.56% vs 30.00%; P>0.05). The Constant scores were improved in the two groups 1 month, 3 months and 12 months after surgery, and the Constant scores in the observation group were higher than those in the control group, with statistically significant differences between the two groups(P<0.05). Conclusion　Perioperative progressive comprehensive rehabilitation intervention can continuously improve the active range of motion and Constant score of the affected shoulder joint after radical mastectomy, which is beneficial to the recovery of upper extremity function.]]> 2022/9/1 11:53:33 CAO Wan, YANG Jian-rong, ZOU Quan-qing, et al. 527true ［Abstract］　Objective　To observe the effect of acupoint injection of oxytocin on preventing postpartum hemorrhage. Methods　One hundred and fifty pregnant women with high risk factors of uterine inertia who gave birth in Guigang Hospital of Traditional Chinese Medicine from February 2019 to October 2020 were selected and divided into an observation group and a control group by random number table method, with 75 cases in each group. In the third stage of labor, the observation group was injected with 5 U of oxytocin at Hegu point, and the control group was given intramuscular injection of 20 U of oxytocin. The volume of postpartum hemorrhage, the levels of hemoglobin and red blood cell count, and the incidence rates of uterine inertia and postpartum hemorrhage were compared between the two groups. Results　The bleeding volume in the observation group was less than that in the control group 2 hours and 24 hours after delivery, and the difference was statistically significant(P<0.05). The levels of hemoglobin and red blood cell count in the observation group were higher than those in the control group 24 hours after delivery, and the differences were statistically significant(P<0.05). The incidence rates of uterine inertia and postpartum hemorrhage caused by uterine inertia in the observation group were lower than those in the control group, and the differences were statistically significant(12.00% vs 25.33%, 5.33% vs 16.00%; P<0.05). Conclusion　The injection of oxytocin at Hegu point can effectively enhance the strength of postpartum uterine contractions, and can effectively prevent the occurrence of postpartum hemorrhage caused by uterine inertia.]]> 2022/9/1 11:53:33 YANG Jin-fei, PAN Ya-jing 526true ［Abstract］　Objective　To investigate the expression of cancer-testis antigen NY-SAR-35 in colorectal cancer(CRC) tissues and its clinical significance. Methods　Fifty-nine patients with CRC who received surgical treatment in Department of General Surgery, the First Affiliated Hospital of Guangxi Medical University from October 2009 to May 2012 were selected. Among the patients, there were 42 males and 17 females, and the age of the patients ranged from 30 to 86 years, with an average age of (55.55±14.36)years; 38 cases were in TNM stage Ⅰ-Ⅱ, and 21 cases in TNM stage Ⅲ-Ⅳ. The cancer tissues and matched paracancerous tissues were obtained by surgery. Reverse transcription-polymerase chain reaction(RT-PCR) was used to detect the expression of NY-SAR-35 mRNA in the tissues. The correlation between the expression of NY-SAR-35 mRNA in the CRC tissues and the clinical characteristics and prognosis of the patients was analyzed. Results　The positive expression rate of NY-SAR-35 mRNA in the CRC tissues was significantly higher than that in the paracancerous tissues(35.59% vs 13.56%, P<0.05). The proportion of the patients with tumor diameter ≥5 cm in the NY-SAR-35 mRNA positive group in the CRC tissues was significantly greater than that in the negative group(P<0.05), but there were no significant differences between the two groups in gender, age, tumor location, carcinoembryonic antigen(CEA) level, T stage, N stage, M stage, TNM stage, histological type and degree of differentiation(P>0.05). The survival prognosis of the NY-SAR-35 mRNA negative group was better than that of the NY-SAR-35 mRNA positive group(survival periods: 57.24 months vs 40.71 months; log-rank test: χ²=4.356, P=0.037). The results of Cox regression analysis showed that T_3-4 of T stage, N_1-3 of N stage, M₁ of M stage, and the positive expression of NY-SAR-35 mRNA were the independent risk factors affecting the survival prognosis of the CRC patients(P<0.05). Conclusion　NY-SAR-35 mRNA is highly expressed in CRC tissues and is associated with poor prognosis of the patients, and has potential value as a therapeutic target for CRC.]]> 2022/7/30 18:40:21 WANG Yan-jing, SHEN Ning, LUO Bin, et al. 525true ［Abstract］　Objective　To investigate the effects of standardized intrapartum antibiotic prophylaxis( IAP) on maternal and infant outcomes in pregnant women colonized with Streptococcus agalactiae［also known as group B streptococcus(GBS)］. Methods　The clinical data of 176 GBS-positive pregnant women who underwent obstetric examination and vaginal trial labor in Fujian Provincial Hospital South Branch from January 2021 to December 2021 were retrospectively analyzed. All of the pregnant women received antibiotic prophylaxis near the time of labor or after rupture of membranes. According to the timeliness and duration of antibiotic use, the pregnant women were divided into IAP standard group(n=124) and IAP non-standard group(n=52). The amniotic fluid contamination, the incidence of chorioamnionitis, as well as neonatal inflammatory indicators, neonatal 1-minute Apgar score, birth weight and incidence of infection events were compared between the two groups. Results　There were no significant differences in the incidence of amniotic fluid turbidity and the incidence of chorioamnionitis between the IAP standard group and the IAP non-standard group(12.10% vs 17.31%, 2.42% vs 1.92%; P>0.05). The incidence of the neonates admitted to neonatal intensive care unit(NICU), the incidence of neonatal pneumonia, the incidence of neonatal sepsis, white blood cell count and neutrophil ratio in the IAP standard group were lower than those in the IAP non-standard group, and the differences were statistically significant(P<0.05). Conclusion　Standardized IAP can reduce the incidence of neonatal adverse events in pregnant women with GBS colonization in the third trimester.]]> 2022/7/30 18:40:21 LIN Rong, JIE Xiao-ting, WENG Xiao-ying 524true ［Abstract］　Objective　To study the differential expressions of atrial natriuretic peptide(ANP) in physiological and pathological myocardial hypertrophy tissues, and its role in the occurrence and development of myocardial hypertrophy. Methods　Fifteen specific pathogen-free(SPF) grade 8-week-old male Sprague-Dawley(SD) rats were selected and randomly divided into control group(CON group), isoproterenol group(ISO group) and exercise group(EXE group), with 5 rats in each group. In the ISO group, the pathological myocardial hypertrophy model was established by subcutaneous injection of isoproterenol at multiple points in the neck. Physiological myocardial hypertrophy was induced by aerobic exercise(swimming) in the EXE group. The CON group received no special intervention. At the end of the 8th week, the rats were sacrificed by overdose anesthesia, and the heart tissues were harvested. Hematoxylin and eosin(HE) staining was used to observe the pathological and physiological changes of heart tissues. Western-blot and immunohistochemical staining were used to detect the expressions of ANP in the myocardial tissues in the three groups. Results　Compared with those in the CON group, the myocardial fibers in the EXE group and the ISO group were significantly thickened, and the ISO group also had the characteristics of structural damages such as widening of myocardial fiber gaps and disordered arrangement. However, the myocardial fibers in the EXE group were neatly arranged and structurally intact. ANP was mainly expressed in the cytoplasm of myocardial cells. The expression level of ANP in the ISO group was higher than that in the EXE group and the CON group, and the differences were statistically significant(P<0.05). Conclusion　The expression of ANP is elevated in pathological myocardial hypertrophy tissues and ANP can be used as an auxiliary indicator to distinguish physiological myocardial hypertrophy from pathological myocardial hypertrophy.]]> 2022/7/30 18:40:21 LI Zheng-kuan, SUN Zhan, BAO Ya-li, et al. 523true ［Abstract］　Objective　To analyze the correlation of serum CXC chemokine ligand 10(CXCL10) and its receptor chemokine(C-X-C motif) receptor 3(CXCR3) with the proportion of CD27⁺CD4⁺ T cells in peripheral blood mononuclear cells(PBMC) in patients with active pulmonary tuberculosis. Methods　One hundred patients with active pulmonary tuberculosis who were admitted to Henan Chest Hospital from April 2019 to April 2021 were selected as the pulmonary tuberculosis group, and 96 healthy people who underwent physical examination during the same period were selected as the control group. The expression levels of serum CXCL10, CXCR3, interferon-γ(IFN-γ) and tumor necrosis factor-α(TNF-α) in the subjects were detected by using enzyme-linked immunosorbent assay(ELISA). The level of CD27⁺CD4⁺ T cells in PBMC was detected by using flow cytometry. Pearson correlation analysis was used to investigate the correlation between serum CXCL10 and CXCR3 levels and CD27⁺CD4⁺ T cell level in PBMC, serum IFN-γ level and TNF-α level. The receiver operating characteristic(ROC) curve was used to analyze the diagnostic efficiency of serum CXCL10 and CXCR3 in active pulmonary tuberculosis. Results　The serum levels of CXCL10, CXCR3, IFN-γ and TNF-α in the pulmonary tuberculosis group were higher than those in the control group, and the differences were statistically significant(P<0.05). The proportion of CD27⁺CD4⁺ T cells in PBMC in the pulmonary tuberculosis group was lower than that in the control group, and the difference was statistically significant［(22.65±2.53)% vs (41.57±5.32)%; t=31.997, P=0.000］. The results of Pearson correlation analysis showed that the serum CXCL10 of the patients with active pulmonary tuberculosis was positively correlated with the levels of CXCR3, IFN-γ and TNF-α(P<0.05), and was negatively correlated with the proportion of CD27⁺CD4⁺ T cells in PBMC(P<0.05). The level of serum CXCR3 was positively correlated with the levels of serum IFN-γ and TNF-α(P<0.05), and was negatively correlated with the proportion of CD27⁺CD4⁺ T cells in PBMC(P<0.05). The results of ROC curve analysis showed that both serum CXCL10 and CXCR3 had application value in the diagnosis of active pulmonary tuberculosis(AUC=0.825, AUC=0.820; P<0.05), and the combination of the two indicators could further improve the diagnostic efficiency of active pulmonary tuberculosis(AUC=0.938, P=0.000), and the sensitivity and specificity were 94.00% and 91.70%, respectively. Conclusion　The levels of serum CXCL10 and CXCR3 are up-regulated in patients with active pulmonary tuberculosis, which may be related to the low proportion of CD27⁺CD4⁺ T cells. The combined detection of the two indicators can assist in the diagnosis of active pulmonary tuberculosis.]]> 2022/7/30 18:40:21 LI Jian-jun, WU Su-fang, BAI Feng-xi 522true ［Abstract］　Objective　To investigate the application effect of intravenous anesthesia through precava in Bama mini-pigs for medium or minor operations. Methods　Fifteen 6-month-old conventional Bama mini-pigs were selected, including 7 females and 8 males, with average weight being (23.17±0.35)kg. After intramuscular injection of 0.1-0.15 mg/kg of midazolam injection for 10 to 15 minutes, a central venous catheter was inserted 8-10 cm into the anterior vena cava of the Bama mini-pigs through the supraclavicular fossa approach to facilitate blood drawing and drug administration. Twenty milligrams of propofol medium/long chain fat emulsion injection were intravenously injected, followed by intravenous injection of propofol medium/long chain fat emulsion injection to maintain anesthesia at 20-40 mg/h. Sufentanil injection was administered intravenously at 2 μg for analgesia. During anesthesia, the pigs were given oxygen inhalation, and their body temperature, electrocardiogram, and percutaneous oxygen saturation were monitored. Results　Reliable anesthetic effects were obtained in the 15 Bama mini-pigs, including 1 case of cholecystotomy under gastroscope, 1 case of internal orifice irrigation of appendix under enteroscope, 1 case of middle cerebral artery electrocoagulation through frontotemporal approach, and 12 cases of left common carotid artery ligation+left ascending pharyngeal artery air injection. During the anesthesia period, the Bama mini-pigs did not shake their ears and did not shake their heads, and their vital signs were stable. The rectal temperature of the Bama mini-pigs was 38.3-38.9 ℃; the heart rate was 76.0-107.0 beats/min; the respiration rate was 13.0-42.0 breaths/min, and percutaneous oxygen saturation(SpO₂) was 95.0%-100.0%. Conclusion　The created total-intravenous anesthesia using propofol medium/long chain fat emulsion injection combined with sufentanil injection through precava provides convenience for medium or minor operations in Bama mini-pigs.]]> 2022/7/30 18:40:21 LI Ping-ping, DENG Da-zhi, HUANG Xue-mei 521true ［Abstract］　Objective　To analyze the clinical effect of femoral artery combined with innominate artery and left common carotid artery cannulation on treatment of patients with acute type A aortic dissection(ATAAD). Methods　Sixty-two ATAAD patients who underwent surgical treatment in Provincial Hospital Affiliated to Anhui Medical University from April 2021 to April 2022 were selected. Among the 62 patients, 26 patients undergoing the 3-arterial cannulation of femoral artery+innominate artery+left common carotid artery to establish cardiopulmonary bypass(CPB) were selected as the observation group, and 36 patients undergoing the double-arterial cannulation of femoral artery+innominate artery to establish CPB were selected as the control group. The preoperative, intraoperative and early postoperative clinical data were compared between the two groups. Results　Compared with the control group, the observation group had shorter circulatory arrest time and greater intraoperative blood loss, and the differences were statistically significant(P<0.05). The postoperative recovery time, ventilator deactivation time and chest tube removal time in the observation group was shorter than that in the control group, and the intensive care time and postoperative hospitalization time in the observation group was shorter than that in the control group, and the incidence rates of brain complications and renal insufficiency in the observation group were lower than those in the control group, and the drainage volume on the day after operation in the observation group was greater than that in the control group, and the differences were statistically significant(P<0.05). In the control group, there were 3 cases(8.33%) with secondary thoracotomy for hemostasis, and 3 cases(8.33%) died within 30 days after operation. In the observation group, there were no cases with secondary thoracotomy for hemostasis, and 1 case(3.85%) died within 30 days after operation, and there was no significant difference between the two groups(P>0.05). Conclusion　In the surgical treatment of ATAAD, CPB can be safely and effectively established through the cannulation of the femoral artery+innominate artery+left common carotid artery, which can ensure sufficient blood supply to the brain during the operation while reducing the time of circulatory arrest and the risk of postoperative complications, shortening the postoperative hospitalization time, and improving the prognosis of the patients, which is worthy of clinical promotion.]]> 2022/7/30 18:40:22 WANG Chen, GE Jian-jun 520true ［Abstract］　Objective　To investigate the association between triglyceride-glucose(TyG) index and in-stent restenosis(ISR) after percutaneous coronary intervention(PCI) in patients with coronary heart disease. Methods　The clinical data of 243 patients with coronary heart disease who were admitted to Department of Cardiology, the Second Affiliated Hospital of Kunming Medical University from January 2016 to September 2021 were collected through the hospital electronic medical record system, including 179 males and 64 females, with an average age of (60.92±11.13)years. All the patients underwent percutaneous coronary intervention(PCI) and their postoperative follow-up was completed. According to the occurrence of in-stent restenosis(ISR) during the follow-up period, the patients were divided into ISR group(45 cases) and non-ISR group(198 cases). The general clinical data, operation-related indexes, and biochemical indexes before and after PCI were compared between the two groups. The risk factors of ISR after PCI in patients with coronary heart disease were analyzed by binary logistic regression. Results　The proportion of smoking patients and diabetes patients in the ISR group was higher than that in the non-ISR group, and the difference was statistically significant(P<0.05). There were no statistically significant differences between the two groups in age, gender, complicated hypertension, body mass index(BMI), Gensini score, total number of implanted stents, diameter of implanted stents, length of implanted stents, multivessel disease, and diffuse disease(P>0.05). Before operation, the levels of total cholesterol(TC), triglyceride(TG), fasting blood glucose(FBG) and TyG indexes in the ISR group were higher than those in the non-ISR group, and the differences were statistically significant(P<0.05). During the re-examination of angiography, the level of TyG index and the proportion of the patients who did not meet the standard of TyG index in the ISR group were greater than those in the non-ISR group, and the differences were statistically significant(P<0.05). The results of binary logistic regression analysis showed that smoking(OR=6.592), complicated diabetes mellitus(OR=3.843), and substandard TyG index at re-examination(OR=23.273) were the independent risk factors for ISR after PCI in the patients with coronary heart disease(P<0.05). Conclusion　Elevated TyG index may increase the risk of ISR in patients with coronary heart disease after PCI, and the management of TyG index control should be strengthened in the patients after PCI.]]> 2022/7/30 18:40:22 CHEN Ling-ling, LI Bo 519true ［Abstract］　Objective　To observe the changes of soft and hard tissues, long axis of teeth after extracted camouflage fixed treatment for adult skeletal class Ⅱ malocclusion patients. Methods　Twenty-four adult skeletal class Ⅱ malocclusion patients who had been extracted four premolars for camouflage fixed treatment at the People′s Hospital of Guangxi Zhuang Autonomous Region during 2002 and 2019 were selected. The 15 cephalometric measurement parameters of the patients including SNA angle, SNB angle, ANB angle, FMA angle, SN-MP angle, U1-NA angle, U1-NA distance, U1-SN angle, L1-NB angle, L1-NB distance, L1-MP angle, U1-L1 angle, Ls-E line distance, Li-E line distance and Z angle were compared before and after treatment, and were compared with the normal reference values. The correlation between the variation of the L1-NB distance and the variation of the other 14 parameters was analyzed. Results　Compared with those before treatment, the SNA angle, SNB angle, U1-NA angle, UI-NA distance, U1-SN angle, L1-NB distance, Ls-E line distance and Li-E line distance of the patients decreased after treatment, and the SN-MP angle, U1-L1 angle and Z angle of the patients increased after treatment, and the differences were statistically significant(P<0.05). Before treatment, the SNB angle, U1-L1 angle, and Z angle of the patients were smaller than the normal reference values, and the ANB angle, SN-MP angle, L1-NB distance, L1-MP angle, Ls-E line distance and Li-E line distance were larger than the normal reference values, and the differences were statistically significant(P<0.05). After treatment, the SNB angle, U1-NA angle, UI-NA distance, U1-SN angle, and Z angle of the patients were smaller than the normal reference values, and the ANB angle, SN-MP angle, L1-MP angle, U1-L1 angle and Ls-E line distance were larger than the normal reference values, and the differences were statistically significant(P<0.05). Before and after treatment, the △L1-NB distance was positively correlated with the △U1-NA angle, △U1-NA distance, △U1-SN angle, △Ls-E line distance, △Li-E line distance, △L1-NB angle and △L1-MP angle(P<0.05), and was negatively correlated with the △U1-L1 angle and △Z angle(P<0.05). Conclusion　Compared with those before treatment, the upper and lower lip soft tissues and the long axis of the lower incisor are improved, and the long axis of the upper incisor is slightly retroclined than normal, but the improvement of hard tissues is not significant after the extracted camouflage fixed treatment for adult skeletal class Ⅱ malocclusion. The adduction of the upper incisor affects the lip inclination and backward displacement of anterior teeth of the upper and lower jaws, and the protrusion of upper and lower lips.]]> 2022/7/30 18:40:22 WANG Ling, HUANG Min-fang, RAO Ming-cong, et al. 518true ［Abstract］　Objective　To compare the clinical effects of two different types of disposable circumcision suture devices in circumcision. Methods　From August 2019 to October 2021, the clinical data of 230 patients who underwent circumcision using a disposable circumcision suture device(DCSD) at Department of Urology, the Affiliated Hospital of Qingdao Binhai University and Department of Urology, the Third People′s Hospital of Qingdao were retrospectively analyzed. According to the patients′ wishes to receive the different types of DCSD, they were divided into the observation group(129 cases) and the control group(101 cases). The observation group was treated with DCSD and sutured with single-wide anastomotic nails. The control group was treated with DCSD and sutured with anastomotic nails combined with silicone pad. The operation time, intraoperative blood loss, pain scores 2 hours after operation, pain scores of the first dressing change after operation, the degree of edema 10 days after operation, the time of complete nail removal, the number of cases with incomplete nail removal 1 month after operation, the incidence of postoperative hematoma, the incidence of postoperative infection, the incidence of incision dehiscence and the patients′ satisfaction 3 months after operation were compared between the two groups. Results　There were no significant differences between the two groups in the results of the operation time, the intraoperative blood loss and the pain scores 2 hours after operation(P>0.05). The pain scores of the first dressing change after operation and the degree of edema 10 days after operation in the observation group were significantly lower than those in the control group(P<0.05). The results of the time of complete nail removal and the number of cases with incomplete nail removal 1 month after operation in the control group were significantly better than those in the observation group(P<0.05). There were no significant differences in the incidence of postoperative hematoma and the incidence of incision dehiscence between the two groups(P>0.05). The incidence of postoperative infection in the observation group was significantly lower than that in the control group(P<0.05). There was no significant difference in the patients′ satisfaction between the two groups 3 months after operation(P>0.05). Conclusion　Compared with the DCSD of single-wide anastomotic nails, the DCSD of anastomotic nails combined with silicone pad sutures significantly improves the situation of nail removal, but the disadvantages of the latter method are that the rate of short-term edema and the rate of infection are higher, and that the pain scores during dressing change are higher. However, the long-term effects of the two groups are similar.]]> 2022/7/30 18:40:22 LI Kui, SUN Yan-bo, WANG Feng-yao, et al. 517true ［Abstract］　Objective　To analyze the risk factors of massive cerebral infarction in patients after intracranial aneurysm surgery, and to improve clinicians′ understanding of the disease. Methods　The clinical data of 238 patients with intracranial aneurysm who were admitted to Hezhou People′s Hospital from January 1, 2010 to December 31, 2020 were retrospectively analyzed. Among the 238 patients, 131 patients received interventional embolization and 107 patients underwent craniotomy and clipping. Thirteen patients developed massive cerebral infarction after surgery(the poor prognosis group), and the rest 225 patients had a good prognosis(the good prognosis group). Multivariate logistic regression was used to analyze the risk factors of massive cerebral infarction in the patients after intracranial aneurysm surgery. Results　Compared with the good prognosis group, the poor prognosis group had a greater proportion of the patients with World Federation of Neurosurgical Societies(WFNS) grades Ⅳ to Ⅴ, modified Fisher grade Ⅳ, multiple aneurysm sacs(MSA) aneurysm type and irregular carotid aneurysm. Compared with those in the good prognosis group, the incidence rates of intraoperative aneurysm clip remodeling, temporary clipping and aneurysm rupture in the poor prognosis group were higher. The maximum diameter of the aneurysm and the size ratio in the poor prognosis group were larger than those in the good prognosis group, and the differences were statistically significant(P<0.05). The results of multivariate logistic regression analysis showed that larger aneurysm diameter(OR=1.052) and irregular carotid aneurysm(OR=3.413) were the independent risk factors for postoperative cerebral infarction(P<0.05). Conclusion　Irregular carotid aneurysm and large aneurysm diameter are the risk factors for postoperative massive cerebral infarction in the patients with intracranial aneurysm. Clinicians should pay attention to such patients to reduce the risk of postoperative adverse events.]]> 2022/7/30 18:40:22 ZHU Guang-sheng, HUANG Hui-xia, CHEN Bin, et al. 516true ［Abstract］　Objective　To investigate the feasibility of stromal vascular fraction(SVF)-loaded methacrylate gelatin(GelMA) hydrogel in promoting the regeneration of subcutaneous vascularized nipple-shaped cartilage. Methods　SVF derived from rabbit fat was extracted, and the component cells of SVF were cultured in vitro, and their adipogenic, osteogenic and chondrogenic differentiation abilities were detected. Then, SVF was uniformly loaded into GelMA hydrogel, and the influence of loaded SVF on the printability of GelMA hydrogel was verified by comparing with single GelMA hydrogel. Thereafter, GelMA and GelMA/SVF composite hydrogels were loaded with chondrocytes respectively, and were subcutaneously implanted into nude mice for 4 weeks. The regeneration of cartilage specific extracellular matrix in the two groups of hydrogel samples was compared by hematoxylin staining(HE) and Safranin-O staining, and the vascularization in the two groups of hydrogel samples was compared by CD31 immunofluorescence staining. Results　Fat-derived SVF component cells had good proliferative ability, and could be differentiated into adipocytes, osteoblasts and chondrocytes. The GelMA/SVF composite hydrogel had comparable three-dimensional(3D) printing performance with single GelMA hydrogel. Four weeks after subcutaneous implantation in nude mice, the regenerated cartilage in the GelMA/SVF composite hydrogel group exhibited superior homogeneous and rich cartilage specific extracellular matrix deposition compared with that in the GelMA hydrogel group, and the expressions of cartilage specific biochemical components(including GAG and collagen Ⅱ contents) in the GelMA/SVF composite hydrogel group were higher than those in the GelMA hydrogel group, and the vascularized expression in the GelMA/SVF composite hydrogel group was better than that in the GelMA hydrogel group. Conclusion　The introduction of SVF can significantly promote GelMA hydrogel to achieve subcutaneous vascularized nipple-shaped cartilage regeneration.]]> 2022/7/4 12:13:21 ZHANG Yu, DING Jing-hao, CHEN Ru 515true ［Abstract］　Objective　To prepare polylactide-co-glycolide microspheres loaded with parathyroid hormone-related protein(PLGA-PTHrP) and to observe their morphological characterization and biocompatibility. Methods　The empty polylactide-co-glycolide(PLGA) microspheres were prepared by double emulsion-solvent evaporation method. Parathyroid hormone-related protein(PTHrP) was loaded into the empty PLGA microspheres by soaking empty PLGA microspheres in the different concentrations of PTHrP solution. PLGA microspheres loaded with effective concentration of PTHrP were prepared by freeze-drying method. The morphology of PLGA microspheres was observed by scanning electron microscope and the particle size of microspheres was calculated. The protein release amount of PLGA-PTHrP was detected, and the release curve was drawn. PLGA-PTHrP was co-cultured with rat whole bone marrow cells, and the effect of composite microspheres on cell proliferation and differentiation was analyzed. Results　The empty PLGA microspheres prepared by the double emulsion-solvent evaporation method were spherical or quasi-spherical, with flat surface and average particle size of (56.73±7.22) μm. The results of in vitro protein release experiment of protein loaded microspheres showed that the release rate of protein was faster within the first 100 hours, and the total release time lasted up to 300 hours. The protein released by protein loaded microspheres accounted for 87.3% of the total protein. The experimental results showed that PLGA-PTHrP had no significant effect on the proliferation of activity rat whole bone marrow cells. Conclusion　The PLGA empty microspheres with flatter surface and more uniform particle size can be successfully prepared by double emulsion-solvent evaporation method.The effective PLGA-PTHrP sustained-release system can be prepared by surface soaking and extraction and freeze-drying method and the sustained-release system can realize the sustained-release effect of PTHrP in a certain time range.]]> 2022/7/4 12:13:21 LIU Jin-jing, ZHOU Qiang-qiang, LONG Gui-yue, et al. 514true ［Abstract］　Objective　To compare the differences in heart rate measured by three-lead electrocardiography(ECG) and pulse oximeter(PO) in the early postnatal period of neonates. Methods　Eighty-six neonates born in Affiliated Hospital of Jiangsu University from May 2019 to May 2021 were selected, including 54 males and 32 females. The gestational age was (35.44±3.75)weeks, and the birth weight was (2 497.97±1 004.98)g. Among the neonates, 26 cases were very low birth weight neonates. All the neonates were placed with three-lead ECG and PO at the same time after birth. The time-consuming placement, the time-consuming to obtain stable heart rate, the total time-consuming to detect the heart rate, and the ratio of obtaining stable heart rate in the early stage were compared between the three-lead ECG and PO placements. The changes and differences in the heart rates measured by the two methods within the first 10 minutes were observed in the neonates after birth. Results　The time of three-lead ECG placement was longer than that of PO, and the time to obtain stable heart rate and total time-consuming to measure the heart rate measured by three-lead ECG placement was shorter than that measured by PO, and the differences were statistically significant(P<0.05). For the very low birth weight infants, the three-lead ECG placement took longer time than PO placement, and the time to obtain stable heart rate measured by three-lead ECG placement was shorter than that measured by PO, and the difference was statistically significant(P<0.05). However, there was no statistical difference in the total time-consuming to measure the heart rate between the measurement by the three-lead ECG placement and the measurement by PO(P>0.05). Within the first 30 seconds, the proportions of the neonates who obtained stable heart rate by three-lead ECG and PO were 79.07%(68/86) and 62.79%(54/86), respectively, and the difference was statistically significant(P=0.000). For the very low birth weight infants, the proportions of stable heart rate obtained by three-lead ECG and PO within the first 30 seconds were 46.15%(12/26)and 34.62%(9/26), respectively, and the difference was not statistically significant(P=0.405). Within the first 6 minutes after birth, the neonates′ heart rates measured by PO were lower than those measured by three-lead ECG, and then the two measured values tended to be close. Conclusion　The time required to obtain stable heart rate in three-lead ECG is significantly shorter than that in PO, and the proportion of obtaining stable heart rate within the first 30 seconds in three-lead ECG is higher than that in PO. Within the first 6 minutes in the neonates after birth, the heart rate measured by PO is lower than that measured by three-lead ECG.]]> 2022/7/4 12:13:21 LI Zhi-chao, LU Jun-ying, CHEN Xiao-jun, et al. 513true ［Abstract］　Objective　To analyze the differences and clinical significances of serum carcino-embryonic antigen(CEA) expression levels in gastric cancer patients with different alpha fetoprotein(AFP) expression states. Methods　The clinical data of 346 patients with primary gastric cancer who were consecutively admitted to the Third Affiliated Hospital of Kunming Medical University from January 2012 to December 2017 were retrospectively analyzed. The patients were divided into AFP-positive gastric cancer(AFPGC) group(AFP≥7 μg/L, 28 cases) and AFP-negative gastric cancer(non-AFPGC) group(AFP<7 μg/L, 318 cases), and were further stratified into normal CEA group(CEA<3.4 μg/L) and CEA elevated group(CEA≥3.4 μg/L). The clinicopathological characteristics and survival prognosis were compared between different divided groups. Results　There were no significant differences between the AFPGC group and the non-AFPGC group in age, gender, T stage, N stage, M stage, clinicopathological stage, tumor cell differentiation degree, and tumor cell pathological type(P>0.05).There were no significant differences in the expressions of tumor markers of CA125, CA199 and CA724 between the two groups(P>0.05). The proportion of the patients with CEA≥3.4 μg/L in the AFPGC group was significantly higher than that in the non-AFPGC group(P<0.05). The median survival time was 30 months and 31 months in the AFPGC group and the non-AFPGC group, respectively, and there was no significant difference in survival prognosis between the two groups(log-rank: χ²=0.723, P=0.395). In the AFPGC patients, except that the proportion of the male patients in the elevated CEA group was significantly higher than that in the normal CEA group, there were no significant differences in the other indicators of clinicopathological characteristics(P>0.05), and there was no statistically significant difference in the survival prognosis between the two groups(log-rank: χ²=0.267, P=0.605). In the non-AFPGC patients, the proportions of male patients and the proportions of the patients with T stage of T_3～4, N stage of N(+), clinicopathological stage of Ⅲ-Ⅳ, and CA199≥27 ku/L and CA724≥6.9 ku/L in the elevated CEA group were higher than those in the normal CEA group, and the differences were statistically significant(P<0.05). The median survival time was 24 months and 34 months in the elevated CEA group and the normal CEA group, respectively, and the difference in survival prognosis between the two groups was statistically significant(log-rank: χ²=6.658, P=0.001). Conclusion　The establishment of gastric cancer subtypes based on the combined detection of AFP and CEA has certain clinical significance for clinical treatment options and efficacy prediction.]]> 2022/7/4 12:13:21 LUAN Li-kun, DAI You-guo, YANG Chang-long, et al. 512true ［Abstract］　Objective　To explore the mechanism of Ganoderma lucidum in regulating immunological function based on network pharmacology and molecular docking. Methods　The active components of Ganoderma lucidum were retrieved and screened by Traditional Chinese Medicine systems pharmacology database and analysis platform(TCMSP), and the target prediction of the active components was made by Swiss Target Prediction database. The immune-related targets were obtained through GeneCards, DrugBank, OMIM, PharmGKB and TDD databases, and the drug targets and the disease targets were integrated to obtain the intersection targets. Cytoscape software was used to construct the drug-active ingredient-action target-disease network diagram and screen the core components. String platform was used to construct the protein-protein interaction(PPI) network and screen the core proteins. GO enrichment analysis and KEGG pathway analysis were performed by R software. Molecular docking technique were used to verify the molecular docking of the active components and core targets. Results　A total of 43 active components, 499 action targets, 12 454 immune-related targets and 483 drug-disease intersection targets in Ganoderma lucidum were retrieved. KEGG pathway analysis showed that the overlapping genes were mainly enriched in neuroactive ligand-receptor interaction, cAMP signaling pathway, proteoglycans and cancer signaling pathways. GO analysis showed that the overlapping genes were mainly involved in the regulation of lipid metabolic process, phosphorylation of peptidyl-serine, modification of peptidyl-serine and so on. The results of molecular docking showed that the main core components and core proteins had good binding activity. Conclusion　The “component-target-pathway-disease” network of Ganoderma lucidum constructed by research reveals the complex mechanism of action of Ganoderma lucidum with multiple components and targets to a certain extent, and provides a theoretical basis for the clinical application and product development of Ganoderma lucidum.]]> 2022/7/4 12:13:21 QIN Jun-liang, SU Zhi-heng 511true ［Abstract］　Objective　To investigate the application value of inferior vena cava collapsibility index(IVC-CI) in guiding fluid therapy for pulmonary hemorrhage in preterm infants. Methods　Forty-six preterm infants with pulmonary hemorrhage who were admitted to Xiamen Children′s Hospital from July 2016 to June 2020 were selected. Among the 46 preterm infants, 22 cases received bedside measurement of IVC-CI(the observation group), and the other 24 cases did not receive bedside measurement of IVC-CI(the control group). On the basis of routine treatments(including mechanical ventilation and haemostasis), the observation group was combined with IVC-CI dynamic assessment to evaluate the volume status for guiding fluid therapy; the control group was given empirical fluid therapy. The fluid intake and the improvement time of lactic acid and oxygenation index were compared between the two groups during the observation period. Results　The results of Pearson correlation analysis showed that IVC-CI was positively correlated with the declined degree of Hb in the observation group(r=0.859, P=0.000). The fluid intake of the observation group was higher than that of the control group during the period from 0 hours to 5 hours and 59 minutes after the discovery of pulmonary hemorrhage, while the fluid intake of the observation group was lower than that of the control group from 6 hours to 23 hours and 59 minutes after the discovery of pulmonary hemorrhage, and the differences were statistically significant between the two groups(P<0.05). There was no significant difference in fluid intake between the two groups from 24 hours to 48 hours(P>0.05). The time when lactic acid returned to normal and oxygenation index decreased by 50% in the observation group was shorter than that in the control group, and the difference was statistically significant(P<0.05). The duration of ventilator treatment in the observation group was shorter than that in the control group, and the difference was statistically significant ［(127.27±34.18)h vs (154.08 ±45.45)h; t=2.245, P=0.003］. Conclusion　The measurement of IVC-CI under the guidance of ultrasound can dynamically assess the volume status, and guide the fluid therapy of preterm infants with pulmonary hemorrhage in a targeted manner, and contribute to the recovery of the disease.]]> 2022/7/4 12:13:21 JIN Hong-juan, LIU Xiao-yan, HUANG Ying 510true ［Abstract］　Objective　To analyze the efficacy of hip arthroplasty in treatment of femoral intertrochanteric fractures after proximal femoral nail antirotation(PFNA) internal fixation failure. Methods　A retrospective analysis was performed on 21 patients with femoral intertrochanteric fractures after PFNA internal fixation failures who received hip arthroplasty treatment from October 2014 to January 2019, including 8 cases of total hip arthroplasty and 13 cases of femoral head replacement. The operative time, intraoperative blood loss, postoperative drainage volume, complications, Harris score and excellent and good rate were recorded. The survival of the prostheses was observed by X-ray. Results　The average operative time of hip arthroplasty in the 21 patients with PFNA failed operation was (120.242±35.623)min, and their intraoperative blood loss and postoperative drainage volume were (520.252±212.363)ml and (320.362±180.248)ml, respectively. Iatrogenic fracture occurred in 2 cases during operation and superficial wound infection occurred in 1 case after operation, but no deep vein thrombosis(DVT) was found in all the patients. Nineteen cases were followed up for 1 to 3 years. At the last follow-up, no loosening or fracture of the prostheses was found in the X-ray films of all the patients. Compared with those before operation, Harris score and excellent and good condition of the hip joints were significantly improved after follow-up(P<0.05). Conclusion　Hip arthroplasty is a feasible remedial treatment for the failure of PFNA internal fixation of femoral intertrochanteric fractures. It needs to strictly understand the operation indications. Fully evaluating the disease preoperatively and gently operating on the patient intraoperatively are conducive to reducing the incidence of complications.]]> 2022/7/4 12:13:21 LI Fu-lin, YIN Dong, HUANG Yu, et al. 509true ［Abstract］　Objective　To explore the surgical treatment methods and results in 13 patients with scalp squamous cell carcinoma(SCC) invading periosteum. Methods　The data(including medical records, images, pathology, etc.) of 13 patients with scalp SCC invading periosteum and undergoing surgical treatment were collected from Gulou Clinical Medical College Affiliated to Nanjing University of Chinese Medicine during January 2010 and December 2020. The surgical treatment methods were skin tumor resection+skull outer plate grinding+local skin flap transfer+skin grafting. Results　All the 13 patients recovered well after surgery. After one year of follow-up, 10 patients were in good condition with no signs of recurrence and metastasis, and the curative effect was satisfactory. One patient had multiple recurrences and metastases, and died half a year later. Two patients had poor survival in the graft area, and healed well after the second-stage operation. Conclusion　When scalp SCC invades periosteum, partial or complete removal of the outer skull plate can effectively reduce the recurrence of the tumor.]]> 2022/7/4 12:13:21 XU Yu-wei, CAO Yun, WEN Bo, et al. 508true ［Abstract］　Objective　To compare the clinical effects between intrauterine placement of Mirena and oral administration of Diane-35 after transcervical resection of endometrial polyps. Methods　The medical records of 61 patients with endometrial polyps admitted to Wuzhou Red Cross Hospital from July 2019 to July 2020 were retrospectively collected, and all the patients underwent hysteroscopic electric resection. The patients were divided into the Mirena group(intrauterine placement of Mirena, n=30) and the Diane-35 group(oral administration of Diane-35, one tablet each time, once a day, for 21 consecutive days, repeated for 6 cycles, n=31) according to their postoperative treatments. The improvement of menstrual period, thickness of uterine membrane, recurrence rate and incidence of adverse reactions were compared between the two groups after operation. Results　The effective rate of the Mirena group was higher than that of the Diane-35 group, but the difference was not statistically significant(96.67% vs 77.42%; χ²=3.411, P=0.065). There was no significant difference in endometrial thickness between the Mirena group and the Diane-35 group before operation and 3 months after operation(P>0.05). The endometrial thickness of the Mirena group was thinner than that of the Diane-35 group 6 months and 12 months after operation, and the differences were statistically significant(P<0.05). During the observation period of 12 months after operation, the total recurrence rate of the Diane-35 group was higher than that of the Mirena group, and the difference was statistically significant(25.81% vs 3.33%; χ²=4.466, P=0.035). The incidence of postoperative adverse reactions in the Mirena group was significantly lower than that in the Diane-35 group(13.33% vs 38.71%; χ²=5.074, P=0.024). Conclusion　Compared with oral administration of Diane-35, intrauterine placement of Mirena can more effectively reduce the recurrence rate after endometrium electroresection of polyps, and has lower incidence of adverse reactions.]]> 2022/7/4 12:13:21 WU Wan-ying 507true ［Abstract］　Objective　To investigate the association of cholyglycine(CG), total bile acid(TBA) and serum 25-hydroxyvitamin D₃［25(OH)Vit D₃］ with the adverse pregnancy outcomes in patients with intrahepatic cholestasis of pregnancy(ICP). Methods　From January 2019 to April 2020, a total of 153 patients who were diagnosed with ICP and treated in Citic Huizhou Hospital were collected and divided into mild ICP group(114 cases) and severe ICP group(39 cases). One hundred and fifty-three healthy pregnant women who received antepartum examination in the same period were included as healthy control group. After blood samples were collected, CG, TBA, liver enzymes and 25(OH)Vit D₃ were detected, and the patients were followed up for pregnancy outcomes. Results　The serum levels of CG, TBA and liver enzymes in the ICP pregnant women were significantly higher than those in the healthy pregnant women, and increased with the increase of disease severity, and the serum level of 25(OH)Vit D₃ decreased with the increase of disease severity, and the differences were statistically significant(P<0.05). The level of 25(OH)Vit D₃ was negatively correlated with CG and TBA in the pregnant women(P<0.05). The gestational weeks of the ICP pregnant women were less than those of the pregnant women in the healthy control group, and the incidence of adverse pregnancy outcomes in the ICP group was higher than that in the healthy control group, and the differences were statistically significant(P<0.05). Conclusion　The levels of CG, TBA and liver enzymes in ICP pregnant women are higher than those in healthy pregnant women. The levels of 25(OH)Vit D₃ in ICP pregnant women are lower than those in healthy pregnant women. High levels of CG, TBA and low levels of 25(OH)Vit D₃ have adverse effects on perinatal pregnant outcomes.]]> 2022/7/4 12:13:21 HUANG Yu-lin, LI Wei-qing, LI Hui-chan, et al. 506true ［Abstract］　Objective　To investigate the regulatory effect of tumor necrosis factor α(TNF-α) on the expression of small non-coding ribonucleic acids(sncRNAs) in human bone marrow mesenchymal stem cells(hBMSCs). Methods　Five patients with congenital hip dysplasia who received surgical treatment in Department of Orthopedics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine from August 2021 to October 2021 were selected. hBMSCs were isolated by whole bone marrow blood adherence method. The TNF-α group was intervened with complete medium containing 10 ng/ml TNF-α, and the control group was added with an equal volume of complete medium. The total RNA of hBMSCs was extracted after 24 hours of intervention, and the expression of sncRNAs was detected by small RNA sequencing, and verified by real-time quantitative polymerase chain reaction(PCR). Results　After intervention with TNF-α, the expressions of 5 micro-ribonucleic acids(miRNAs)(hsa-novel-170-mature, hsa-miR-155-5p, hsa-miR-1260a, hsa-novel-202-mature, hsa-novel-116-mature) and 4 PIWI-interacting RNAs(piRNAs)(DQ592932, DQ570992, DQ593325, DQ582827) in hBMSCs were up-regulated(P<0.05), and 4 miRNAs(hsa-miR-141-3p, hsa-miR-3065-5p, hsa-novel-155-mature, hsa-miR-27a-5p), 13 small nucleolar RNAs(snoRNAs)(SNORD14D, SNORD116-3, SNORA21, SNORD60, SNORD81, SNORD32A, SNORD117, SNORD2, SNORD1C, SNORD36B, SNORD4B, SNORA58, SNORA64) and 1 small nuclear RNA(snRNA)(NR_003137.2) were down-regulated(P<0.05). Conclusion　TNF-α can regulate the expressions of various sncRNAs in hBMSCs, thereby affecting the osteogenic differentiation of hBMSCs.]]> 2022/6/10 17:57:16 ZHOU Xing, LU Yan-qing, PENG Jian-ping, et al. 505true ［Abstract］　Objective　To analyze the clinical characteristics of middle-aged and elderly female patients with insomnia under the concept of Traditional Chinese Medicine(TCM) syndrome differentiation. Methods　A total of 144 middle-aged and elderly female patients with insomnia aged 45-75 years who were admitted to the outpatient department of Shanghai Seventh People′s Hospital from March 1, 2016 to January 31, 2020 were selected as the research subjects. The indexes of age, insomnia related symptoms, location and nature of diseases and syndrome differentiation were analyzed. Results　In the 144 patients, the average age was (59.43±7.96) years, including 69 patients(47.92%) aged 45-59 years and 75 patients(52.08%) aged 60-75 years. The characteristics of insomnia in the middle-aged and elderly women were easy to wake up(55.56%), difficult to sleep(53.47%) and early to wake up(20.83%); the main accompanying symptoms were epigastric discomfort(59.03%), dizziness and fatigue(53.47%) and chest tightness and upset(52.08%). The main disease locations of insomnia were spleen(60.42%), heart(50.00%) and liver(43.75%). The main disease natures were Qi deficiency(42.36%), Qi stagnation(31.25%) and phlegm dampness(20.83%). The syndrome differentiations of insomnia mainly included the syndrome of heart and liver fire excess(28.47%), the syndrome of heart and spleen deficiency(18.75%), and the syndrome of phlegm dampness blocking collaterals(11.81%). The results of statistical analysis showed that the clinical characteristics of insomnia with symptoms of dizziness and fatigue, disease location of heart, disease nature of phlegm dampness, and syndrome type of deficiency of both heart and spleen, phlegm dampness blocking collaterals were associated with the age of the middle-aged and elderly female insomnia patients(P<0.05). Conclusion　Insomnia in middle-aged and elderly women is related to the decline of physiological function and the deficiency of Zang Fu organs. Early intervention and early diagnosis and treatment are conducive to the prevention and treatment of senile intractable insomnia.]]> 2022/6/10 17:57:16 SHEN Ding-yi, LI Xiao-pan, MENG Fan-ping, et al. 504true ［Abstract］　Objective　To observe the sedative effect of white noise combined with chloral hydrate on neonatal lumbar puncture. Methods　Seventy neonates who were admitted to Shunde Hospital of Southern Medical University from November 2020 to October 2021 for lumbar puncture were selected and divided into an observation group and a control group by random number table method, with 35 cases in each group. The control group was given chloral hydrate 50 mg/kg diluted with self-sucking milk 30 minutes before lumbar puncture, and the observation group was intervened with white noise on the basis of the same treatment as the control group. The success rate of lumbar puncture, Neonatal Infant Pain Scale(NIPS) score during lumbar puncture needle insertion, and Brussels sedation score and vital signs at different time points before and after intervention were compared between the two groups. The occurrence of adverse reactions in the two groups was observed. Results　The NIPS scores of the observation group at the time of lumbar puncture were significantly lower than those of the control group［(3.34±1.39)points vs (4.37±2.46)points; t=2.151, P=0.036］. The Brussels sedation scores in both groups showed a downward trend with time, and the changes in the observation group were greater(P<0.05). The blood oxygen saturation in both groups showed an upward trend with the progress of the intervention time, while the systolic blood pressure, heart rate and respiratory rate showed a downward trend(P<0.05). The decreases of heart rate and respiratory rate in the observation group were more significant than those in the control group(P<0.05). There were no significant differences in the success rate of lumbar puncture and the success rate of one-needle puncture between the observation group and the control group(91.43% vs 88.57%, 65.71% vs 62.86%; P>0.05). There were no adverse reactions such as bradycardia, hypotension and decreased blood oxygen saturation in both groups. Conclusion　White noise combined with chloral hydrate has significant sedative and analgesic effects on neonatal lumbar puncture, with good safety and promotion value.]]> 2022/6/10 17:57:16 YOU Min-yi, HUANG Ren, KANG Si-mei, et al. 503true ［Abstract］　Objective　To explore the effects of long non-coding ribonucleic acid(lncRNA) highly up-regulated in liver cancer(HULC) on micro ribonucleic acid(miRNA) differential expression profile and competitive endogenous ribonucleic acid(ceRNA) regulatory network of liver cancer cells. Methods　Human liver cancer cell line BEL-7402 was selected, and HULC siRNA was transfected instantaneously into human liver cancer cell line BEL-7402 to reduce the expression of lncRNA HULC. The different expressions of miRNA were selected by using high-throughput sequencing and were verified by real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR). The new miRNA was predicted based on reference genomes. Gene enrichment analysis and ceRNA regulatory network were used to study the potential function of these genes. Results　After the expression of lncRNA HULC was knocked down, nine significantly differentially expressed miRNAs were selected by high-throughput sequencing. The expressions of 5 miRNAs were down-regulated and those of 4 miRNAs were up-regulated. Fifteen new miRNAs with higher scores were selected by using miRDeep2 scoring. The results of gene enrichment analysis showed that the molecular functions of the down-regulated miRNA target genes were related to the activities of serine tRNA ligase, protein kinase, cationic channel and calcium transporter ATPase. The target genes of the up-regulated miRNAs were related to selenocysteine lyase activity, peroxisomal mechanism targeting signal-1 binding, peroxisomal targeting sequence binding, protein N-tail binding, tumor necrosis factor receptor superfamily binding, etc. The miRNA-target gene interaction network and the significantly enriched function-function interaction network revealed the association of target genes and miRNA, and the correlation between functions. Conclusion　In liver cancer cell line BEL-7402, lncRNA HULC has the role of regulating multiple miRNAs with different expressions and has effects on the occurrence and development of liver cancer through the regulation network mode of ceRNA.]]> 2022/6/10 17:57:16 GUAN Lu-lu, HAN Song-feng, LI Shi-peng, et al. 502true ［Abstract］　Objective　To investigate the changes of serum heparanase(HPSE), lectin-like oxidized low density lipoprotein receptor-1(LOX-1) and N-terminal pro-B-type natriuretic peptide(NT-proBNP) levels in patients with acute decompensated heart failure(ADHF) before and after standardized treatment, and to analyze the correlation among HPSE, LOX-1 and NT-proBNP. Methods　Fifty ADHF patients who were admitted to Department of Cardiology, the First Affiliated Hospital of Inner Mongolia University of Science and Technology Baotou Medical College from May 2019 to December 2020 were selected. The levels of serum HPSE, LOX-1 and NT-proBNP were detected before and after standardized treatment, and the correlation among HPSE, LOX-1 and NT-proBNP was explored by Pearson correlation analysis or Spearman correlation analysis. Results　Compared with those before treatment, the levels of serum NT-proBNP, HPSE and LOX-1 in the 50 ADHF patients were all decreased after treatment, and the differences were statistically significant(P<0.05). The results of Spearman correlation analysis showed that HPSE and LOX-1 levels were significantly positively correlated with NT-proBNP level(r_s=0.839, P=0.000; r_s=0.902, P=0.000). The results of Pearson correlation analysis showed that there was a significantly positive correlation between HPSE and LOX-1 levels(r=0.761, P=0.000). Conclusion　HPSE and LOX-1 may be involved in the occurrence and development of heart failure, and they have application prospects for evaluating the severity and prognosis of ADHF patients.]]> 2022/6/10 17:57:16 WANG Shi-qi, WANG Meng-qin, HAN Xuan-mao, et al. 501true ［Abstract］　Objective　To explore the application value of sensory nerve quantitative detector in evaluating the degree of nerve root injuries before and after musculoskeletal ultrasound-guided blade needle and traditional blade needle in the treatment of cervical spondylotic radiculopathy. Methods　One hundred and twenty patients with cervical spondylotic radiculopathy admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2018 to November 2019 were selected, and they were divided into group A and group B by random number table method, with 60 cases in each group. In the group A, 5 cases lost, and 55 cases were eventually included. In the group B, 7 cases lost, and 53 cases were eventually included. The group A was treated with musculoskeletal ultrasound-guided blade needle, and the group B was treated with traditional blade needle. The sensory nerve quantitative detector was used to evaluate the nerve injuries of the affected sides of the two groups before and after treatment, and these nerve injuries were compared between the two groups. Results　Under the detection conditions of 2 000 Hz, 250 Hz and 5 Hz, there were no significant differences in the quantitative detection results of the affected side sensory nerves(C₆, C₇, and C₈) of the patients in the two groups before treatment(P>0.05). After treatment, the sensory nerve functions of C₆, C₇, and C₈ in the two groups were improved compared with those before treatment(P<0.05), and the grade of the quantitative detection results of sensory nerve in the group A was lower than that in the group B, and the difference was statistically significant(P<0.05). Conclusion　Sensory nerve quantitative detector can objectively evaluate the degree of nerve root injury, which has a certain reference significance for guiding the follow-up treatment.]]> 2022/6/10 17:57:16 GAO Xin, YANG Jian-rong, WANG Cheng, et al. 500true ［Abstract］　Objective　To explore the application value of phase angle(PA) measurement in assessing the nutritional status of hemodialysis(HD) patients. Methods　Ninety-eight patients who received HD treatment in Department of Nephrology, the First Affiliated Hospital of Hainan Medical University from January 2019 to December 2020 were selected. The patients ranged in age from 23 to 72(45.5±5.3)years, including 64 males and 34 females. Among the patients, there were 36 cases of diabetic nephropathy, 29 cases of chronic glomerulonephritis, 11 cases of hypertensive nephropathy, 2 cases of chronic interstitial nephritis, 2 cases of lupus nephritis, 1 case of adult polycystic kidney disease, and 17 cases of unknown cause. The nutritional status of the HD patients was assessed by malnutrition-inflammation score(MIS). The PA was measured within 20 minutes after HD was completed by using the InBody770 body composition analyzer, and the PA value under the condition of 50 kHz was taken as the result. The receiver operator characteristic(ROC) curve method was used to analyze the efficacy of PA measurement in evaluating the nutritional status of the patients by using MIS as the gold standard. The correlations between PA measurements and the clinical indicators of body weight, upper arm muscle circumference, serum albumin, prealbumin, serum total iron-binding capacity(TIBC) and transferrin were analyzed. Results　Among the 98 patients, the cases of normal nutrition, mild malnutrition, moderate malnutrition and severe malnutrition were 9 cases(9.18%), 34 cases(34.69%), 45 cases(45.92%) and 10 cases(10.20%), respectively, and the incidence of malnutrition was 90.82%(89/98). The results of ROC curve analysis showed that PA(50 kHz) could better distinguish normal nutrition from mild malnutrition, mild malnutrition from moderate malnutrition, and moderate malnutrition from severe malnutrition［area under the curve(AUC)(95%CI)=0.902(0.772-0.971), P=0.000; AUC(95%CI)=0.665(0.550-0.767), P=0.016; AUC(95%CI)=0.702(0.564-0.818), P=0.004］. The results of Spearman rank correlation analysis showed that PA(50 kHz) measurements was significantly positively correlated with the patients′ body weight, upper arm muscle circumference, prealbumin and TIBC(P<0.05), and was significantly negatively correlated with age and dialysis time(P<0.05). Conclusion　The incidence of malnutrition in HD patients is high. PA measurement can detect malnutrition in the patients early, which is beneficial for clinicians to provide nutritional intervention in a timely manner and improve the quality of life of the patients.]]> 2022/6/10 17:57:16 LIU Nan, LIANG Hai-qin, WANG Ting-ting, et al. 499true ［Abstract］　Objective　To analyze the prenatal diagnosis and pregnancy results of 17 patients carrying chromosomal translocation. Methods　The clinical data of 17 patients diagnosed with chromosomal translocation carriers by prenatal examination at the Prenatal Diagnosis Center of Huaihua City Maternal and Child Health Care Hospital from January 2018 to December 2021 were collected. All the fetuses underwent chromosome karyotype and chromosome microarray analysis(CMA). The diagnostic results and pregnancy outcomes of the patients were summarized. Results　Among the 17 chromosomal translocation carriers, 14 cases were maternal and 3 cases were paternal. 14 cases had a history of embryo arrest or fetal structural abnormalities; 1 case was the first pregnancy with fetal structural abnormalities; 2 cases were the first pregnancy［assisted reproductive embryo preimplantation genetic test(PGT)］, and normal babies were obtained. Fifteen cases involved 2 non-homologous chromosomes, and 2 cases involved more than 3 complex chromosome rearrangements(CCR). Prenatal diagnosis of fetal karyotype and CMA results showed 7 cases of unbalanced translocation, 9 cases of balanced translocation, and 1 case of normal karyotype(via assisted reproductive PGT). The pregnancy outcomes: 8 cases of induced labor, 9 cases of normal childbirth. Conclusion　Chromosomal translocation carriers have a high risk of adverse pregnancy outcomes, and prenatal diagnosis should be made to exclude unbalanced translocations and reduce birth defects.]]> 2022/6/10 17:57:16 YI Feng-mei, FENG Zong-hui, JIANG Shu-min, et al. 498true ［Abstract］　Objective　To analyze the difference and consistency of the measurement of white to white(WTW) and anterior chamber depth(ACD) in myopia patients using three different instruments: IOLMaster700, Pentacam and Orbscan. Methods　Fifty-one patients(102 eyes) who underwent binocular implantable collamer lens(ICL) implantation in Department of Ophthalmology, the People′s Hospital of Guangxi Zhuang Autonomous Region were selected from April 2019 to December 2019. The patients′ WTW and ACD were measured by IOLMaster700, Pentacam and Orbscan, and the difference and consistency of the results measured by the three instruments were analyzed. Results　There were statistically significant differences in the WTW results measured by the three instruments(P<0.05). Among them, the results measured by IOLMaster700 were significantly greater than those measured by Pentacam and Orbscan(P<0.05), and there was no statistically significant difference between the WTW results measured by Pentacam and Orbscan(P>0.05). There was no statistically significant difference among the ACD results measured by the three instruments(P>0.05). Intra-class correlation coefficient(ICC) analysis results showed that the ICC of WTW measured by the three instruments ranged from 0.790 to 0.896, and the ICC of ACD measured by the three instruments ranged from 0.952 to 0.966, indicating that the measurement results of the three instruments were well consistent. The results of Bland-Altman analysis showed that Pentacam and Orbscan had good consistency in measuring WTW, but IOLMaster700 and Pentacam, as well as IOLMaster700 and Orbscan had poor consistency in measuring WTW. The results of ACD measured by the three instruments were well consistent. Conclusion　The WTW measurements of IOLMaster700 are significantly greater than those of Pentacam and Orbscan, and it is not recommended to use the three methods in measurement of WTW interchangeably. Pentacam and Orbscan have good consistency in the measurement of WTW, which can be referred to each other clinically. IOLMaster700, Pentacam and Orbscan have smaller differences in ACD measurements and have good consistency in the measurement of ACD.]]> 2022/6/10 17:57:16 LI Lan-jian, JIANG Li, LYU Jian, et al. 497true ［Abstract］　Objective　To investigate the value of combined detection of neutrophil to lymphocyte ratio(NLR) and plasma fibrinogen(FIB) in diagnosis of gastric cancer. Methods　One hundred and sixteen gastric cancer patients admitted to Suzhou Municipal Hospital from June 2019 to March 2022 were selected as the gastric cancer group, and 82 healthy people receiving physical examination were selected as the control group during the same period. The NLR and FIB levels were compared between the two groups. The correlations of the NLR and FIB levels with TNM stage and lymph node metastasis in the gastric cancer patients were analyzed. The receiver operator characteristic(ROC) curve method was used to evaluate the efficiency of NLR, FIB and their combination in diagnosing gastric cancer. Results　The levels of NLR and FIB in the gastric cancer group were higher than those in the control group, and the differences were statistically significant(P<0.05). For the gastric cancer patients, there were no significant correlations of the NLR and FIB levels with their TNM stages and lymph node metastases(P>0.05). The results of ROC curve analysis showed that the area under the curve(AUC) values of NLR, FIB and their combination in the diagnosis of gastric cancer were 0.692, 0.650 and 0.738, respectively. The sensitivity and specificity of the combined diagnosis of the two indicators for gastric cancer were 69.80% and 70.70%, respectively, and their diagnostic efficiency was better than that of each indicator alone. Conclusion　The combined detection of NLR and FIB has certain value in the diagnosis of gastric cancer, which can improve the diagnostic efficiency of gastric cancer and is helpful for the early screening of gastric cancer.]]> 2022/6/10 17:57:16 CAO Chen-liang, WANG Qing 496true ［Abstract］　Objective　To analyse the clinicopathological features and prognosis of patients with abdominal wall implantation metastasis of gastrointestinal adenocarcinoma. Methods　The clinical data of 11 patients with abdominal wall implantation metastasis of gastrointestinal adenocarcinoma who were admitted to Weihai Central Hospital Affiliated to Qingdao University from July 2017 to December 2020 were retrospectively analyzed, and their pathological findings and clinical prognosis were summarized. Results　The primary diseases of the 11 patients were gastric cancer in 4 cases and intestinal cancer in 7 cases. The lesion site of gastric cancer in the 4 cases was distal stomach. Among the 7 cases of intestinal cancer, the focus was located in the right colon in 5 cases, the left colon in 1 case and the sigmoid colon in 1 case. Among the 11 patients, the histopathological examination results of the primary tumors in 10 patients showed moderately to poorly differentiated adenocarcinoma, and the pathological stage of 9 patients was T₃ and above. When the 11 patients were found to have recurrence of abdominal wall metastatic cancer, the time from their first operation was 2-49 months, and the median time was 17.9 months, which had no significant correlation with whether the patients underwent radiotherapy and chemotherapy after operation. The histopathological morphology of the metastatic tumors was almost identical to that of the primary tumors. The survival time of the patients with abdominal wall metastasis was 2-20 months, and the median survival time was 8.7 months. One patient was lost to follow-up, and another patient survived so far, and the rest of the patients died due to extensive tumor metastasis. Conclusion　The histological morphology of the implanted metastatic tumors in the abdominal wall is almost identical to that of the primary tumors. Poor differentiation and later pathological staging of the primary tumors indicate that the tumors have entered an advanced stage. It is recommended that the patients undergo regular follow-up and physical examination after surgery to detect implanted metastatic lesions as soon as possible. Clinicians should formulate an appropriate treatment plan according to a patient′s histopathological findings and systemic conditions.]]> 2022/6/10 17:57:16 HOU Guo-qi, TANG Yan, LI Ying-ming 495true ［Abstract］　Objective　To investigate the application effect of multi-mode neuroelectrophysiological monitoring in intraspinal space-occupying surgery. Methods　Forty-five patients with intraspinal space-occupying lesions who were admitted to Department of Orthopaedics, Hefei Hospital Affiliated to Anhui Medical University from May 2019 to April 2021 were selected, and were divided into an observation group(23 cases) and a control group(22 cases) according to their willingness to receive neuroelectrophysiological monitoring during surgery. In the observation group, somatosensory evoked potential(SEP), motor evoked potential(MEP) and free-run electromyography(f-EMG) were used for multi-modal combined monitoring during the operation, while the control group did not receive neurophysiological monitoring. The postoperative intraspinal space-occupying resection rate, Japanese Orthopaedic Association(JOA) score and McCormick grade were compared between the two groups. The intraoperative multi-mode neuroelectrophysiological monitoring and postoperative neurological damage in the observation group were analyzed. Results　The intraspinal space-occupying resection rate in the observation group was higher than that in the control group, and the difference was statistically significant(91.30% vs 54.55%; χ²=7.770, P=0.001). The postoperative JOA scores in both groups showed an upward trend, and the increase was greater in the observation group(P<0.05). The JOA scores of the observation group were higher than those of the control group, and the differences were statistically significant 3 months and 6 months after operation(P<0.05). On the third day after operation, there was no significant difference in the McCormick grading between the two groups(P>0.05). The McCormick grading in both groups was improved, and the McCormick grading in the observation group was better than that in the control group 6 months after operation(P<0.05). In the observation group, except for 1 patient who had a monitoring alarm and did not recover, and his neurological symptoms worsened after surgery, the other 22 patients who did not have a monitoring alarm or recovered from the monitoring alarm did not have neurological damage after surgery. Conclusion　Multi-mode nerve electrophysiological monitoring helps to improve the therapeutic effect and nerve function recovery of intraspinal space-occupying surgery.]]> 2022/6/10 17:57:16 WEI Xiao-fei, JIA Qi-yu 494true ［Abstract］　Objective　To analyze the development status of social living ability and neuropsychological development level in children with functional articulation disorders(FAD) and their correlation. Methods　A total of 87 FAD children who were admitted to Women′s and Children′s Hospital Affiliated to Xiamen University from June 2020 to May 2021 were selected, with average age of (4.38±0.47)years. The “Infant-Junior High School Students′ Social Living Ability Scale” and the “0-6 Years Old Children′s Neuropsychological Development Checklist” were used to evaluate the children′s social living ability and neuropsychological development level, respectively. Pearson correlation analysis was used to explore the correlation between the scores of each item in the two scales. Results　The overall level of social living ability and various ability grades of the 87 FAD children were mainly normal, and the top three marginal and defect proportions were communication(27/87, 31.03%), self-direction(25/87, 28.74%), occupation(18/87, 20.69%). In terms of neuropsychological development, the proportions of critical and low fine motors were relatively high in the 87 FAD children, reaching 31.03% and 32.19%, respectively. The critical and low proportions of language, adaptability and social behavior were 52.87%(46/87), 49.43%(43/87) and 48.28%(42/87), respectively. For gross motor, the proportion of normality was relatively high(75.86%, 66/87). The average developmental quotients(DQ) of fine motor, adaptability and language were all below 86, which belonged to a low level. The results of Pearson correlation analysis showed that the total score of social living ability was negatively correlated with gross motor level(P<0.05), and positively correlated with fine motor, adaptive ability, language, social behavior and total DQ(P<0.05). In the evaluation of various living abilities, the correlation between independent living items and neuropsychological development level was the most significant(fine motor: r=0.657; adaptive ability: r=0.642; language: r=0.709; social behavior: r=0.861; total DQ: r=0.831). Conclusion　Children with FAD not only have problems in articulation, but also their social living ability and neuropsychological development level are affected, and there is a close relationship between social living ability and neuropsychological development level, which needs to be paid attention to.]]> 2022/6/10 17:57:16 LIU Xiao-ying, YANG Yi-na, LIU Wen-long 493true ［Abstract］　Objective　To compare the prognostic effects of rhythm control and heart rate control therapies in heart failure with preserved ejection fraction(HFpEF) patients complicated with atrial fibrillation(AF). Methods　The clinical data of 150 HFpEF patients complicated with AF admitted to Anhui Provincial Hospital Affiliated to Anhui Medical University from January 2017 to September 2019 were retrospectively collected. The patients were divided into rhythm control group(receiving rhythm control therapy protocol, 75 cases) and heart rate control group(receiving heart rate control therapy protocol, 75 cases) according to their treatment intentions. The clinical prognoses(including all-cause death, cardiovascular death and hospitalization for heart failure) within 24 months of follow-up were compared between the two groups. The levels of amino-terminal pro-B-type natriuretic peptide precursor(NT-proBNP), left ventricular ejection fraction(LVEF) and pulmonary artery systolic pressure(PASP) were compared between the two groups before treatment and after 12 months of treatment. Results　During the follow-up period, there were 3 cases(4.00%) of all-cause death in the rhythm control group, and 9 cases(12.00%) in the heart rate control group, with no significant difference between the two groups(HR=0.309, P=0.119). There were 3 cases(4.00%) in the rhythm control group who died of cardiovascular events, and 6 cases(8.00%) in the heart rate control group, with no significant difference between the two groups(HR=0.443, P=0.280). There were 5 cases(6.67%) who were hospitalized due to heart failure in the rhythm control group, and 19 cases(25.33%) in the heart rate control group, and the difference was statistically significant(HR=0.296, P=0.001). After 12 months of treatment, the levels of NT-proBNP and PASP in the rhythm control group were significantly lower than those before treatment(P<0.05). The levels of NT-proBNP, and PASP in the rhythm control group were lower than those in the heart rate control group, and the differences were statistically significant(P<0.05). Conclusion　Compared with heart rate control therapy, rhythm control therapy can reduce the risk of hospitalization for heart failure in HFpEF patients complicated with AF, but the prognosis of all-cause death and the prognosis of cardiovascular death were not significantly different between the two groups.]]> 2022/4/29 9:03:57 DU Chun-yu, HU Li-qun, LI Hong-qi 492true ［Abstract］　Objective　To investigate the effects of long non-coding ribonucleic acid(lncRNA) gastric carcinoma high expressed transcript 1(GHET1) on the proliferation, migration and invasion of human gastric carcinoma cells MGC-803 and their mechanisms. Methods　MGC-803 cells were used as the experimental subjects. The MGC-803 cell models with lncRNA GHET1 overexpression and lncRNA GHET1 silencing were constructed by lentiviral transfection, which were used as the overexpression group and the silence group, respectively, and the corresponding negative control groups were set. Lentiviral transfection efficiency was observed by fluorescence microscopy. The expression levels of lncRNA GHET1 and micro ribonucleic acid-105(miR-105) in each group were detected by real-time fluorescence quantitative polymerase chain reaction(PCR). The cell proliferation ability of each group was detected by plasma cholecystokinin-8(CCK-8) assay. Cell migration and invasion abilities were detected by Transwell assay. Results　Under fluorescence microscope, the viral transfection rate in the cells in each group was higher than 90%, and the results of real-time fluorescence quantitative PCR showed that the relative expression of lncRNA GHET1 in the overexpression group was significantly higher than that in the overexpression control group(P<0.05), and the relative expression of lncRNA GHET1 in the silence group was significantly lower than that in the silence control group(P<0.05), indicating that the MGC-803 cell models of the overexpressing lncRNA GHET1 and the silencing lncRNA GHET1 were successfully established. The results of CCK-8 experiment showed that the proliferation rate of the cells in the overexpression group was faster than that in the overexpression control group, while the proliferation rate of the cells in the silence group was slower than that in the silence control group. The results of Transwell experiment showed that the number of migrating and invasive cells in the overexpression group was more than that in the overexpression control group, while the number of migrating and invasive cells in the silence group was less than that in the silence control group, and the differences were statistically significant(P<0.05). The results of real-time fluorescence quantitative PCR showed that the expression level of miR-105 in the overexpression group was significantly lower than that in the overexpression control group(P<0.05), and the expression level of miR-105 in the silence group was significantly higher than that in the silence control group(P<0.05). Conclusion　lncRNA GHET1 can promote the proliferation, migration and invasion of human gastric carcinoma cells MGC-803, and their mechanisms may be related to the regulation of the expression level of miR-105.]]> 2022/4/29 9:03:57 TANG Mu-xi, LAI Ming-yu, CHENG Ruo-xi 491true ［Abstract］　Objective　To compare the staining effects of six staining methods at the early, middle and late stages of mandibular distraction osteogenesis in beagles, so as to provide reference for researchers to select the appropriate staining methods. Methods　One-year-old male beagles were selected for surgical modeling after anesthesia. The tissues of the distraction osteogenesis area were taken respectively at the early, middle and late stages of distraction osteogenesis in beagles after euthanasia. After decalcification, the sections were stained by hematoxylin and eosin(HE) staining, Masson staining, methylene blue-acidic magenta staining, Von Kossa staining and modified alizarin red S staining, and modified safranin-O fast green staining, respectively, and the effects of staining were compared. Results　HE staining could be used to observe the basic structure of the distraction osteogenesis area. The Masson staining showed sharp color contrast and clear structure, which could clearly distinguish collagen fibers from muscle fibers and mature bones from new bones. The modified safranin-O fast green staining and the methylene blue-acidic magenta staining were poorly differentiated at the early and middle stages of the distraction osteogenesis, but the two staining methods could clearly distinguished mature bones from new bones at the late stage of distraction osteogenesis. The Von Kossa staining and the modified alizarin red S staining were not very effective because the samples had been decalcified. Conclusion　For the staining study of the distraction osteogenesis area, Masson staining has better staining effects at the early, middle and late stages of distraction osteogenesis. The modified safranin-O fast green staining and the methylene blue-acidic magenta staining are only suitable for the late stage of distraction osteogenesis. The Von Kossa staining method and the modified alizarin red S staining method are not suitable for the staining of tissue sections after decalcification.]]> 2022/4/29 9:03:57 ZHAO Zhen-chen, JIANG Wei-dong, SHI Heng-lei, et al. 490true ［Abstract］　Objective　To screen molecular markers related to cervical cancer immunity based on bioinformatics methods. Methods　The data of 24 cases of normal cervical tissues and 28 cases of cervical cancer tissues were obtained from Gene Expression Omnibus(GEO) database and the data of 306 cases of cervical cancer tissues and 3 cases of normal cervical tissues were obtained from The Cancer Genome Atlas(TCGA) database. Immune and stroma scores were performed on the data set by ESTIMATE algorithm of R software, and the relationship between the scores and the prognosis of cervical cancer was analyzed. Differentially expressed genes(DEGs) were screened out according to the immune scores, and their up- and down-regulations were displayed by Venn diagram, and the intersection of the two databases was taken. GO enrichment analysis, KEGG pathway analysis and gene set enrichment analysis(GSEA) were performed on key genes. Protein-protein interaction(PPI) network was constructed. The expression levels of key genes were analyzed by using GEPIA2 and UALCAN online websites and their correlation with survival prognosis of the patients was verified. Results　Based on the data analysis of the TCGA database, the survival of the patients in the high immune score group was better than that in the low immune score group(log-rank test: χ²=4.400, P=0.035). Based on the data of the TCGA database, a total of 1 063 DEGs were obtained by screening according to the level of immune score, including 640 up-regulated genes and 423 down-regulated genes. A total of 1 903 DEGs were screened out based on the data of the GEO database, including 1 698 up-regulated genes and 205 down-regulated genes. Finally, 7 down-regulated genes and 100 up-regulated genes were obtained from the intersection of the two genes. The 107 DEGs were enriched for analysis, and GO enrichment analysis indicated that DEGs were involved in the biological function of immune regulation. KEGG pathway analysis and GSEA results indicated that DEGs were mainly enriched in cytokine receptor interaction signaling pathway and cell adhesion molecule pathway. Thirteen key genes(the number of nodes ≥8) were further screened out by PPI network, and immune cell correlation analysis found that they were significantly correlated with B cells, T cells and other immune cells(P<0.05). The results of survival analysis showed that among these 13 genes, only SELL and CXCL9 were associated with the survival prognosis of cervical cancer patients(P<0.05). The analysis results of GEPIA2 and UALCAN online websites showed that CXCL9 was highly expressed in cervical cancer tissues(P<0.05). However, only the analysis results of UALCAN showed that the survival prognosis of the CXCL9 high expression group was better than that of the CXCL9 low expression group(P<0.05). Conclusion　Cervical cancer DEGs are involved in the process of immune regulation. CXCL9 is highly expressed in cervical cancer and is associated with the prognosis of cervical cancer patients, and may become a potential therapeutic target for cervical cancer patients.]]> 2022/4/29 9:03:57 LUO Yong-jin, HU Xiao-xia, WANG Dan, et al. 489true ［Abstract］　Objective　To analyze the diagnostic performances of B-ultrasound examination and hysteroscopy for the nature of endometrial lesions in patients with postmenopausal bleeding(PMB). Methods　The clinical data of 211 patients who underwent surgery in the Department of Gynecology of Lanzhou University Second Hospital due to PMB from September 2015 to September 2017 were retrospectively collected. All the patients received B-ultrasound examination, hysteroscopy and pathological examination. According to the nature of the patients′ endometrial lesions, they were divided into non-malignant lesion group(152 cases) and malignant tumor group(59 cases). The clinical characteristics were compared between the two groups. The diagnostic performances of B-ultrasound examination and hysteroscopy were compared by using the pathological examination results as the gold standard. Receiver operator characteristic(ROC) curve analysis was used to investigate the performances of the two examinations in diagnosing the nature of endometrial lesions. Results　Compared with the non-malignant lesion group, the malignant tumor group had older age, longer menopause years, thicker endometrium, and higher proportions of complicated hypertension and diabetes mellitus, and the differences were statistically significant(P<0.05). The sensitivity and specificity of B-ultrasound in diagnosing endometrial polyps were 63.83% and 96.34%, respectively; the sensitivity and specificity in diagnosing submucosal myoma of uterus were 48.57% and 99.43%, respectively; the sensitivity and specificity in diagnosing endometrial malignant tumors were 11.86% and 96.05%, respectively. The sensitivity and specificity of hysteroscopy in diagnosing endometrial polyps were 97.87% and 96.95%, respectively;the sensitivity and specificity in diagnosing endometrial malignancies were 96.61% and 96.71%, respectively; the sensitivity and specificity in diagnosing simple hyperplasia endometrium and(or) complex hyperplasia endometrium were 50.00% and 98.95%, respectively; the sensitivity and specificity in diagnosing endometritis were 93.33% and 99.49%, respectively; the sensitivity and specificity in diagnosing submucosal myoma of uterus were 91.43% and 98.86%, respectively. The results of ROC curve analysis showed that endometrial thickness had the value of diagnosing endometrial malignancies［AUC(95%CI)=0.877(0.828-0.926), P=0.001］, with the best cut-off value being 5.50 mm, the sensitivity being 71.19% and the specificity being 88.16%. Conclusion　Older age, longer menopause years, abnormal endometrial thickening, complicated hypertension or diabetes are the risk factors for endometrial malignancies in PMB patients. B-ultrasound can be used as a primary screening test for PMB patients. For the PMB patients with endometrial thickness >5.50 mm indicated by B-ultrasound examination, clinicians should be alert to the possibility of endometrial malignancies. For suspected intrauterine lesions, hysteroscopy can be further performed.]]> 2022/4/29 9:03:57 AN Hui-meng, GUO Yu-zhen 488true ［Abstract］　Objective　To explore the diagnostic value of serum D-dimer(D-D) level combined with Caprini score in pulmonary embolism(PE). Methods　Sixty PE patients who visited doctors in Dongguan Hospital Affiliated to Southern Medical University(Dongguan People′s Hospital) from September 2019 to May 2021 were selected as the PE group, and 60 non-PE patients during the same period were selected as the control group. The D-D level, Caprini score, coagulation function and inflammatory response indexes were compared between the two groups. The receiver operator characteristic(ROC) curve method was used to analyze the value of serum D-D level and Caprini score in the diagnosis of PE. Pearson correlation analysis was used to explore the correlation of D-D level and Caprini score with coagulation function and inflammatory response indexes in the PE patients. Results　The serum D-D level, Caprini score, C-reactive protein(CRP) and white blood cell(WBC) levels in the PE group were higher than those in the control group, and the fibrinogen(FIB), activated partial thromboplastin time(APTT) and prothrombin time(PT) levels in the PE group were lower than those in the control group, and the differences were statistically significant(P<0.05). The results of Pearson correlation analysis showed that the D-D level and Caprini score in the PE patients were positively correlated with WBC and CRP(P<0.05), and negatively correlated with APTT, PT and FIB(P<0.05). The results of ROC curve analysis showed that both D-D level and Caprini score had application value in the diagnosis of PE(P<0.05), and the combined diagnostic efficiency of the two indicators was higher, with a sensitivity of 90.3% and a specificity of 87.5%. Conclusion　Serum D-D level combined with Caprini score has better diagnostic efficacy for PE.]]> 2022/4/29 9:03:57 FANG Nian-xin, LIU Zhen-wei, YE Guan-sheng, et al. 487true ［Abstract］　Objective　To investigate the clinical efficacy of medical castration in treatment of prostate cancer complicated with urinary retention. Methods　The clinical data of 32 prostate cancer patients complicated with urinary retention in Jiangbin Hospital of Guangxi Zhuang Autonomous Region from June 2017 to June 2020 were retrospectively analyzed. The patients were 65 to 95 years old, with a median age of 79.6 years. The clinical stages were stage Ⅱ in 2 cases, stage Ⅲ in 11 cases and stage Ⅳ in 19 cases. All the patients were intolerant or unwilling to accept radical surgery, and did not agree to the treatment of urinary retention by transurethral resection of the prostate or cystostomy for urinary diversion. After the doctors communicated with the patients and their family members, the patients and their family members agreed to choose medical castration treatment and used the combination of maximum androgen blockade and α-blocker therapy. The changes of International Prostatic Symptomatic Score(IPSS), quality of life(QOL) score, maximum urinary flow rate(Qmax) and prostate volume(PV) were observed after treatment. Results　After 16 weeks of treatment, the urinary catheters were successfully removed in 26 of the 32 cases(81.25%), and 2 cases died due to the deterioration of severe pneumonia, and 2 cases failed to be further followed up due to the deterioration of their conditions. The IPSS and QOL scores of the 26 patients whose urinary catheters were successfully removed after treatment were lower than those before treatment, and the differences were statistically significant(P<0.05). The Qmax at 4 weeks after catheter removal was significantly higher than that immediately after catheter removal［(11.1±2.8)ml/s vs (1.2±0.3)ml/s; t=22.737, P=0.000］. The PV of the 28 surviving patients was significantly reduced 12 weeks after treatment compared with that before treatment［(43.5±10.2)cm³ vs (76.0±15.8)cm³; t=6.712, P=0.000］. Conclusion　Medical castration therapy can improve voluntary urination in high-risk and elderly prostate cancer patients complicated with urinary retention.]]> 2022/4/29 9:03:57 LING Kai-nan, YANG Jian-rong, LI Bi-jin, et al. 486true ［Abstract］　Objective　To study the clinical efficacy of three-dimensional(3D) mirror printing combined with minimally invasive percutaneous plate osteosynthesis(MIPPO) technology in the treatment of adult displaced mid-clavicular fractures. Methods　Forty-eight cases of adult patients with displaced mid-clavicular fractures admitted to Liaocheng Hospital of Traditional Chinese Medicine from June 2020 to May 2021 were selected and divided into an observation group and a control group by random number table method, with 24 cases in each group. The observation group was treated with 3D mirror printing combined with MIPPO technology, while the control group was treated with traditional open reduction and internal fixation. The operation time, incision length, intraoperative blood loss, times of fluoroscopy, fracture healing time, and postoperative complications were compared between the two groups. The Constant shoulder joint score was compared between the two groups before and after surgery to evaluate the clinical efficacy. Results　Compared with the control group, the observation group had shorter operation time, shorter incision length, less intraoperative blood loss, less times of fluoroscopy and faster fracture healing, and the differences were statistically significant(P<0.05). Compared with those before surgery, the Constant shoulder joint scores in the two groups were significantly improved after surgery(P<0.05), but there were no significant differences in the Constant shoulder joint scores between the two groups before surgery and 6 months after surgery(P>0.05). No postoperative complications were observed in the observation group, while 2 cases of numbness around the incision occurred in the control group. There was no significant difference in the incidence of postoperative complications between the two groups(P=0.489). Conclusion　Both 3D mirror printing combined with MIPPO technology and traditional open reduction and internal fixation can effectively treat displaced mid-clavicular fractures, but 3D mirror printing combined with MIPPO technology has the advantages of less trauma, shorter operation time, less intraoperative blood loss, less times of intraoperative fluoroscopy and faster fracture healing.]]> 2022/4/29 9:03:57 MU Sheng-kai, LIU Shi-kai, HAO Lian-sheng 485true ［Abstract］　Objective　To explore the expressions of distal-less homeobox 3(DLX3) and Syncytin-1 mRNA in placental calcified tissues of patients with gestational diabetes mellitus(GDM) and their significances. Methods　Thirty GDM patients admitted to Department of Obstetrics, the People′s Hospital of Guangxi Zhuang Autonomous Region from September 2015 to September 2018 were selected as the observation group, and 30 healthy pregnant women were selected as the control group during the same period. The general clinical data and the expression levels of DLX3 and Syncytin-1 mRNA(detected by real-time quantitative polymerase chain reaction) in placental calcified tissues were compared between the two groups, and the influencing factors of the occurrence of GDM were explored. Results　The weight gain during pregnancy in the observation group was (11.15±4.03)kg, and that in the control group was (13.23±2.98)kg, and the difference between the two groups was statistically significant(t=2.069, P=0.044). There were 3 cases of macrosomia in the observation group(10.00%) and 1 case in the control group(3.33%), and there was no significant difference between the two groups(χ²=0.268, P=0.605). The expression level of DLX3 mRNA in the observation group was significantly higher than that in the control group(P<0.05), but there was no significant difference in the expression level of Syncytin-1 mRNA between the two groups(P>0.05). Multivariate logistic regression analysis showed that higher DLX3 mRNA expression level was a risk factor for the occurrence of GDM(OR=8.142, P=0.004). Conclusion　DLX3 is a risk factor for promoting the occurrence of GDM, and it may be involved in the occurrence of GDM and placental calcification.]]> 2022/4/29 9:03:57 WU Hua, FANG Xiang, LI Jing, et al. 484true ［Abstract］　Objective　To observe the clinical application effect of pretreatment with dexmedetomidine on intracranial aneurysm clipping. Methods　Eighty patients with intracranial aneurysm admitted to Zhongshan Traditional Chinese Medicine Hospital from June 2019 to May 2021 were selected, and all the patients underwent open aneurysm clipping under general anesthesia and endotracheal intubation. The patients were divided into observation group and control group by random number table method, with 40 cases in each group. The observation group was treated with dexmedetomidine by intravenous pump before anesthesia induction and tracheal intubation, and the control group was treated with the same amount of normal saline. The levels of cerebral oxygen metabolism indexes at the end of surgery were compared between the two groups. The intraoperative doses of propofol, remifentanil and sevoflurane, and the levels of circulatory function-related indicators before extubation were compared between the two groups. Results　At the end of the operation, the intracranial pressure in the observation group was lower than that in the control group, and the cerebral perfusion pressure and cerebral oxygen uptake rate in the observation group were higher than those in the control group, and the differences were statistically significant(P<0.05). The intraoperative doses of propofol, remifentanil and sevoflurane in the observation group were significantly lower than those in the control group(P<0.05). Before extubation, the heart rate in the observation group was slower than that in the control group, and the systolic blood pressure and diastolic blood pressure in the observation group were lower than those in the control group, and the differences were statistically significant(P<0.05). Conclusion　Pretreatment with dexmedetomidine in intracranial aneurysm clipping can improve cerebral perfusion, reduce the dosage of anesthetics, and help to maintain the stability of the patients′ vital signs during surgery and improve surgical safety.]]> 2022/4/29 9:03:57 CHEN Wei-tao, CHEN Zhi-yi, HUANG Hao-ming 483true ［Abstract］　Objective　To investigate the therapeutic effect of atorvastatin on chronic endometritis(CE) in rats and its related mechanisms. Methods　Thirty-two specific pathogen free(SPF) female Sprague-Dawley(SD) rats were selected and randomly divided into sham operation group, model group, low-dose atorvastatin group(10 mg/kg) and high-dose atorvastatin group(20 mg/kg), with 8 rats in each group. The CE rat model was established by intrauterine injection of phenol mucilage. After 14 days of continuous intragastric administration, the rat uterine tissues were collected, and the histopathological changes of the rat uteruses were observed by hematoxylin and eosin(HE) staining. The expression levels of interleukin-1β(IL-1β) and matrix metalloproteinase-9(MMP-9) mRNA in the rat uterine tissues were detected by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR). The expression levels of IL-1β and MMP-9 in the rat uterine tissues were detected by immunohistochemical method. Results　Compared with those in the model group, the endometrial epithelial cell proliferation, degeneration and necrosis, and inflammatory cell infiltration of the rats in the high-dose atorvastatin group were significantly improved. The mRNA levels and protein positive expression rates of IL-1β and MMP-9 in the rat uterine tissues in the low-dose atorvastatin group and high-dose atorvastatin group were lower than those in the model group, and the differences were statistically significant(P<0.05). Conclusion　Atorvastatin can improve the inflammatory response in the CE rats and its mechanisms may be related to down-regulating the expressions of IL-1β and MMP-9.]]> 2022/3/31 9:14:29 XIE Xin, WANG Jia-wei, ZHAO Yun-xiao, et al. 482true ［Abstract］　Objective　To explore the relationship between deafness gene mutation and the postoperative efficacy of cochlear implant(CI) surgery in Guangxi region, and to provide reference for evaluating the postoperative efficacy of CI. Methods　One hundred and four cases of prelingual deafness patients who underwent CI implantation and deafness gene detection in Guangxi region from July 2012 to December 2019 were selected and divided into mutation group(14 cases) and non-mutation group(90 cases) according to their gene mutations. Categories of Auditory Performance(CAP) and Speech Intelligibility Rating(SIR) scores were used to evaluate the efficacy of the patients 3 months, 6 months and 12 months after surgery. Results　Deafness gene mutation was detected in 14 of 104 patients, and the mutation rate was 13.46%. There were 2 cases of GJB2 gene mutation, including 1 case of c.235delC homozygous mutation and 1 case of c.299delC heterozygous mutation. There were 10 cases of SLC26A4 gene mutation, including 8 cases of c.IVS7-2A>G heterozygous mutation, and 2 cases of c.919-2A>G/c.1614+5G>A compound heterozygous mutation. There was 1 case of OTOF gene c.1273C>T/c.4994T>C compound heterozygous mutation. There was 1 case of TRIOBP gene c.5185-2A>G/c.3299C>A compound heterozygous mutation. The CAP and SIR scores showed an upward trend in the patients in the two groups 3 months, 6 months and 12 months after surgery(P<0.05), but there were no significant differences in the changes between the two groups(P>0.05). Conclusion　CI implantation can significantly improve the hearing of patients with prelingual deafness, but the deafness gene mutation has no significant effect on the short-term efficacy of CI implantation.]]> 2022/3/31 9:14:29 HUANG Lan-cheng, LIN Zuan-ping, TANG Feng-zhu, et al. 481true ［Abstract］　Objective　To analyse the correlation of carotid intima-media thickness(cIMT) and blood homocysteine(Hcy) level with the severity of white matter lesions(WML). Methods　Four hundred and twenty-seven WML patients admitted to Department of Neurology, the Third Affiliated Hospital of Southern Medical University from January 2019 to March 2021 were retrospectively collected, and all of them were diagnosed by cranial MRI. The clinical data of the patients were collected through the hospital medical record system, and the patients were divided into mild WML group(1 point, 258 cases) and moderate-severe WML group(≥2 points, 169 cases) according to the Fazekas scale score. The clinical data were compared between the two groups, and the independent risk factors of moderate-severe WML were analyzed by using binary logistic regression, and the value of cIMT and Hcy in evaluating the severity of WML was analyzed by using receiver operator characteristic(ROC) curve. Results　Compared with the mild WML group, the moderate-severe WML group was older, with a higher proportion of male, higher prevalence rates of hypertension, diabetes and coronary heart disease, lower levels of total cholesterol, triglyceride and very low density lipoprotein(VLDL), higher level of Hcy and thicker cIMT, and the differences were statistically significant(P<0.05). The results of binary logistic regression analysis showed that older age, complicated hypertension, complicated diabetes, higher levels of Hcy and right-sided cIMT were the independent risk factors for moderate-severe WML(P<0.05). The results of ROC curve analysis showed that both Hcy level and right-sided cIMT had value in assessing the severity of WML(P<0.05); and the combination of the two indicators was more effective, with a sensitivity of 69.23% and a specificity of 77.52%. Conclusion　Hcy and right-sided cIMT are the independent risk factors for moderate-severe WML, and the combination of the two indicators can effectively assess the severity of WML.]]> 2022/3/31 9:14:29 KONG Ling-tong, YANG Can-hong, CHEN Jia-yi, et al. 480true ［Abstract］　Objective　To analyse the clinical effect of intracavitary interventional therapy on patients with Trans-Atlantic Inter-Society Consensus(TASC)-Ⅱ grade C and grade D lower extremity arteriosclerosis obliterans(ASO). Methods　The clinical data of 52 patients with lower extremity ASO(57 affected limbs) who were treated in the Department of Interventional Vessel and Pain, Hefei Hospital Affiliated to Anhui Medical University and received intracavitary interventional therapy from January 2017 to August 2019 were collected. The surgical success rate, complication rate, patency rate and limb salvage rate of the patients were analyzed. Results　Fifty-two patients(57 affected limbs) underwent 62 times of intracavitary interventional therapy. Among the 57 affected limbs, the occluded segments of 2 affected limbs failed to open successfully after repeated attempts; the operation on 1 affected limb was discontinued due to acute left heart failure in the patient during the operation; 3 affected limbs were transferred to the orthopedics department for amputation, and the success rate of the operation was 94.74%(54/57). Perioperative complications occurred in 2 cases(3.85%), including 1 case of hematoma at the puncture site and 1 case of postoperative ischemia-reperfusion injury in the affected limb, and no perioperative death. The levels of ankle-brachial index(ABI) of the dorsal pedis artery and the posterior tibial artery one month after operation were significantly higher than those before operation(P<0.05). The first-stage patency rates of the affected limbs were 100.00%, 97.96%, 93.88%, 78.72%, 77.78% and 68.18% 1, 3, 6, 12, 18 and 24 months after operation, respectively. The second-stage patency rates 12 and 24 months after operation were 95.35% and 94.44%, respectively. The limb salvage rates 12 and 24 months after operation were 95.74% and 90.90%, respectively. Conclusion　Intracavitary interventional therapy is a safe and effective treatment for lower extremity ASO with longer and more severe lesions.]]> 2022/3/31 9:14:29 LI Jing, YIN Shi-wu, PAN Sheng-quan, et al. 479true ［Abstract］　Objective　To analyse the effect of prenatal antibiotics exposure on intestinal microbiota in late preterm infants. Methods　Thirteen cases of late preterm infants who were hospitalized in the Second Affiliated Hospital of Guangxi Medical University from March 2020 to May 2021 were selected, with gestational age of 34^0/7- 36^6/7 weeks. Among the 13 preterm infants′ mothers, 5 mothers were treated with antibiotics before delivery(the observation group), and the other 8 mothers were not treated with antibiotics before delivery(the control group). Stool specimens of the premature infants were collected on day 3(D3), day 7(D7) and day 14(D14) after birth, and 16SrRNA high-throughput sequencing technology was used to sequence the bacterial V3-V4 regions, and the composition and diversity of the intestinal microbes were compared between the two groups. Results　The Shannon index increased with the age of the late preterm infants in the two groups. The Shannon index of the observation group was always lower than that of the control group. However, there was significant difference in Shannon index between the observation group and the control group only on day 3 after birth(Z=-2.717, P=0.007). With the increase of the age in days of the late preterm infants, the proportion of Proteobacteria in the control group decreased while that in the observation group increased; the proportion of Firmicutes in the control group increased, while that in the observation group decreased. The level of Parabacteroides increased first and then decreased in the observation group, while the level of Parabacteroides was almost undetectable in the control group. Compared with the control group, the observation group had less colonization of Bifidobacterium, and delayed colonization of anaerobic Bacillus, Enterobacter and Kluyverella on day 3 after birth. Conclusion　Prenatal antibiotics exposure can affect the colonization of intestinal microbiota in late preterm infants and reduce the level of intestinal microbial diversity.]]> 2022/3/31 9:14:29 ZHU Yun-lei, HUANG Qing-mei, HUANG Gui-lian, et al. 478true ［Abstract］　Objective　To investigate the effect of plasma amino-terminal pro-brain natriuretic peptide(NT-proBNP) levels on the prognosis of patients with advanced non-small cell lung cancer(NSCLC). Methods　The clinical data of 128 patients with advanced NSCLC who were admitted to Laiyang Central Hospital of Yantai City from January 2013 to June 2016 were retrospectively collected. The survival curve of the patients was plotted by Kaplan-Meier method, and the survival prognoses of the patients under different stratifications were compared［the NT-proBNP>654.5 ng/L group vs the NT-proBNP≤654.5 ng/L group; the Cyfra21-1>3.5 μg/L group vs the Cyfra21-1≤3.5 μg/L group; the stage ⅢB group vs the stage Ⅳ group; the chronic obstructive pulmonary disease(COPD) group vs the non-COPD group; the pleural effusion group vs the non-pleural effusion group］. Cox regression analysis was used to explore the risk factors affecting the survival and prognosis of the patients with advanced NSCLC. The clinical data were compared between the NT-proBNP>654.5 ng/L group and the NT-proBNP≤654.5 ng/L group. Results　The 5-year overall survival rate of the patients with advanced NSCLC in this study was 7.81%. The results of Kaplan-Meier survival curve analysis showed that the NSCLC patients with NT-proBNP>654.5 ng/L, the NSCLC patients with Cyfra21-1>3.5 μg/L, the NSCLC patients with International Association for the Study of Lung Cancer(IASLC) stage Ⅳ, the NSCLC patients complicated with COPD and the NSCLC patients complicated with pleural effusion had worse survival prognosis(P<0.05). The results of Cox regression analysis showed that the pathological type of adenocarcinoma(HR=1.872), IASLC stage Ⅳ(HR=2.910), and higher NT-proBNP level(HR=1.945) were the independent risk factors affecting the survival and prognosis of the NSCLC patients(P<0.05). Compared with the NT-proBNP≤654.5 ng/L group, the NT-proBNP>654.5 ng/L group had higher body mass index(BMI), triglyceride and serum creatinine levels, and the differences were statistically significant(P<0.05). Conclusion　Higher NT-proBNP levels may be an independent risk factor affecting the survival and prognosis of patients with advanced NSCLC, which deserves the attention of clinicians.]]> 2022/3/31 9:14:29 HAO Yun-xia, GAO Shao-ying, XIN Lin-ze, et al. 477true ［Abstract］　Objective　To observe the clinical application effect of multimodal analgesia on patients with lower limb fractures after surgery. Methods　Sixty patients who underwent elective surgery for lower limb fractures from January 2020 to December 2020 in Guangxi Orthopedic Hospital were selected and divided into M group and I group by random number table method, with 30 cases in each group. Multimodal analgesia was used in the M group［(popliteal sciatic nerve block, adductor canal block combined with patient controlled intravenous analgesia(PCIA) pump)］. However, only PCIA pump was used for postoperative analgesia in the I group. The Numeric Rating Scale(NRS) scores, Ramsay sedation scores, and manual muscle testing(MMT) grades were compared between the two groups in repose and activity 6 hours(T₁), 12 hours(T₂), 24 hours(T₃) and 48 hours(T₄) after operation. The incidence of postoperative complications, salvage analgesia rate and satisfaction with analgesia treatment were compared between the two groups. Results　At T₁-T₄ time points, the NRS scores showed an upward trend in the M group and a downward trend in the I group. At T₁-T₃ time points, the NRS scores of the M group were lower than those of the I group, and the differences were statistically significant(P<0.05 ). At T₁-T₄ time points, the Ramsay sedation scores showed a downward trend in the two groups, while the MMT grades showed an upward trend in the two groups, and there were no statistically significant differences in the changing trends between the two groups(P>0.05). There were 4 cases of dizziness and headache, 3 cases of nausea and vomiting in the I group, and no postoperative complications were observed in the M group. There was a statistically significant difference in the rate of postoperative complications between the two groups(23.33% vs 0.00%; χ²=5.822, P=0.016). There was no significant difference in the salvage analgesia rate between the M group and the I group(0.00% vs 10.00%; χ²=1.404, P=0.236). The satisfaction with analgesia treatment in the M group was better than that in the I group(Z=5.054, P=0.000). Conclusion　The multimodal analgesia of popliteal sciatic nerve block, adductor canal block combined with PCIA pump has good analgesic effect and less adverse reactions in patients with lower limb fractures after surgery, which is beneficial to improving the satisfaction of patients with postoperative analgesia.]]> 2022/3/31 9:14:29 CHEN Xi, WU Bin 476true ［Abstract］　Objective　To explore the application value of Sun′s Procedure in elderly patients with acute Stanford type A aortic dissection. Methods　Forty-four patients with acute Stanford type A aortic dissection who received continuous general anesthesia, deep hypothermic circulatory arrest(DHCA) and unilateral selective cerebral perfusion technique and were treated with Sun′s Procedure in Beijing Anzhen Hospital from September 2018 to September 2019 were selected as the study subjects. The patients with age≥60 years were selected as the elderly group(n=9), and those with age<60 years as the young group(n=35). The intraoperative and postoperative situations and the occurrence of adverse events were compared between the two groups. Results　There was no significant difference between the two groups in the time from onset to operation, the time of cardiopulmonary bypass, the time of aortic occlusion and the DHCA time(P>0.05). The intensive care unit(ICU) stay and the total hospital stay in the elderly group were significantly longer than those in the young group(P<0.05). There were no significant differences in the postoperative adverse events between the two groups(P>0.05). Conclusion　The risk of adverse events in elderly patients with acute Stanford A aortic dissection after receiving Sun′s Procedure is similar to that in young patients, but the elderly patients have longer ICU stay and total hospital stay.]]> 2022/3/31 9:14:30 LUO Jin-long, LI Cheng-nan, PANG Feng, et al. 475true ［Abstract］　Objective　To observe the application effect of continuous iliac fascial space block under multimodal analgesia on orthopedic surgery under general anesthesia. Methods　A total of 100 patients undergoing surgery in Department of Orthopedics, Affiliated Hospital of Guangdong Medical University from May 2019 to May 2021 were selected and divided into observation group and control group according to the random number table method, with 50 cases in each group. The control group received traditional continuous iliac fascial space block, and the observation group received continuous iliac fascial space block under multimodal analgesia. The hemodynamic parameters［systolic blood pressure(SBP), diastolic blood pressure(DBP), mean arterial pressure(MAP), heart rate(HR)］, the operation-related indicators(operation time, postoperative wake-up time, consciousness recovery time), the cognitive function［Mini-Mental State Examination(MMSE) score］, the analgesic effect［Visual Analogue Scale(VAS) score］, the sedative effect(Ramsay score), and the occurrence of adverse reactions were compared between the two groups. Results　There were no significant changes in SBP, DBP, MAP and HR between the two groups at the begin of anesthesia(T₁) and before the start of anesthesia(T₀) time points, and the differences were not statistically significant(P>0.05). The levels of the various indicators increased at 30-minute time point after anesthesia(T₂) and at the end of surgery(T₃) time point, but fell back at 12-hour time point after surgery(T₄), and they were basically close to the levels at T₀ time points. In the observation group, the upward floating trend was small and the recovery was rapid(P<0.05). The postoperative wake-up time and consciousness recovery time of the observation group was shorter than that of the control group(P<0.05). MMSE scores in both groups decreased 24 hours after surgery, while those in the observation group decreased more slightly(P<0.05). The VAS scores and the Ramsay scores showed a downward trend in both groups 2, 12, 24 and 48 hours after surgery, and the VAS scores and the Ramsay scores in the observation group were lower than those in the control group(P<0.05). There was no statistically significant difference in adverse reaction rate between the two groups(P>0.05). Conclusion　Continuous iliac fascial space block under multimodal analgesia assisted orthopedic general anesthesia is conducive to stabilizing hemodynamics, improving the effects of sedation and analgesia, promoting postoperative recovery, improving postoperative cognitive function, and has lower adverse reaction rate.]]> 2022/3/31 9:14:30 LIANG Guang-bin, CHEN Cui-ping, YAN Zhen-yi, et al. 474true ［Abstract］　Objective　To explore the expression levels of melanoma-associated antigen(MAGE)-D4 and calnexin(CANX) mRNA in human gliomas, and to analyze their correlation and clinical significances. Methods　The expressions of MAGE-D4 and CANX mRNA in human glioma tissues were analyzed using the data from Gene Expression Profiling Interactive Analysis(GEPIA) database［163 cases of glioblastoma(GBM) tissues, 518 cases of low-grade glioma(LGG) and 207 cases of normal brain tissues］, and were further verified in clinical samples(62 cases of glioma and 11 cases of normal brain tissues) by reverse transcription-quantitative polymerase chain reaction(RT-qPCR). The correlation between the expression levels of MAGE-D4 and CANX mRNA and the clinical indicators of glioma patients was analyzed. Small interfering RNA(siRNA) technology was used to silence the expressions of MAGE-D4 in glioma cell lines SF126 and U251, and the correlation between the expressions of MAGE-D4 and CANX was investigated. Results　The results of data analysis of GEPIA database showed that the expression levels of MAGE-D4 and CANX mRNA in GBM and LGG tissues were higher than those in normal brain tissues, and the differences were statistically significant(P<0.05). The RT-qPCR test results of the clinical samples also showed that the expression levels of MAGE-D4 and CANX mRNA in glioma tissues were significantly higher than those in normal brain tissues(P<0.05). The expression levels of MAGE-D4 and CANX mRNA were not related to the patient′s age, gender, World Health Organization(WHO) grade, pathological type, Karnofsky Performance Score(KPS), the longest diameter of the tumor, and Ki-67, glial fibrillary acidic protein(GFAP), S-100 and vimentin expression levels(P>0.05). The results of Pearson correlation analysis showed that the expression levels of MAGE-D4 and CANX mRNA in the tissues of the clinical glioma samples were positively correlated(r=0.916, P=0.000). The cell experiment results showed that the expression levels of CANX mRNA in SF126 and U251 cells decreased significantly after they were silenced by MAGE-D4 siRNA(P<0.05). Conclusion　MAGE-D4 and CANX are highly expressed in human gliomas, and their expression levels are positively correlated, suggesting that they may participate in the occurrence and development of gliomas, and have the application prospect of becoming targets for immunotherapy of gliomas.]]> 2022/3/8 10:33:08 XIE Huan, ZHANG Qing-mei, ZHANG Peng-bo, et al. 473true ［Abstract］　Objective　To investigate the expressions of caudal-related homeobox transcription factor 2(CDX2) and mucin 2(MUC2) in gastric intestinal metaplasia tissues and their clinical significances. Methods　A total of 152 patients who underwent endoscopic biopsy in Department of Gastroenterology, General Hospital of Ningxia Medical University from January 2011 to May 2014 were selected and divided into non-atrophic gastritis group(32 cases), atrophic gastritis group(31 cases), low-grade intraepithelial neoplasia group(24 cases), high-grade intraepithelial neoplasia group(5 cases), gastric cancer group(17 cases) and gastric intestinal metaplasia(IM) group(43 cases). The expressions of CDX2 and MUC2 in gastric mucosa tissues were detected by immunohistochemistry method, and the serum level of Helicobacter pylori(Hp)-IgG was detected by enzyme-linked immunosorbent assay(ELISA) method. The consistency of CDX2, MUC2 and Hp-IgG detection results was analyzed. Results　There were significant differences in the positive rates of CDX2 and MUC2 among the six groups(P<0.05). Among the six groups, the positive rates of CDX2 and MUC2 in the IM group were the highest, and were significantly higher than those in the other five groups(P<0.05). The positive rate of Hp-IgG in the IM group was higher than that in the gastritis group, and the difference was statistically significant(65.12% vs 42.50%; χ²=4.270, P=0.039). The results of Kappa consistency analysis showed that the expressions of CDX2 and Hp-IgG were consistent in the IM group(P<0.05), but the expressions of CDX2 and Hp-IgG in the gastritis group were not significantly consistent(P>0.05). The expressions of MUC2 and Hp-IgG in the IM group and the gastritis group were not significantly consistent(P>0.05). Conclusion　The positive expression rates of CDX2 and MUC2 in the IM tissues are higher than those in other gastric mucosal tissues. Hp infection may cause the occurrence of IM by inducing the expression of CDX2.]]> 2022/3/8 10:33:08 YANG Jia-li, WANG Zhen, LIU Fei, et al. 472true ［Abstract］　Objective　To observe the effect of resveratrol(Res) on the proliferation and apoptosis of human prostate cancer PC-3 cells, and to explore its mechanism. Methods　The PC-3 cells were used as the experimental subjects and were divided into group A(12.50 μmol/L Res), group B(25.00 μmol/L Res), group C(50.00 μmol/L Res), group D(100.00 μmol/L Res) and control group(equal volume of complete medium) according to different intervention methods. The methyl thiazolyl tetrazolium(MTT) colorimetric method was used to detect the proliferation of the PC-3 cells in each group. Propidium iodide(PI) fluorescence staining was used to observe the changes of the PC-3 cell nuclei in each group. Flow cytometry was used to detect the apoptosis of the PC-3 cells in each group. Real-time quantitative polymerase chain reaction(RT-qPCR) was used to detect the mRNA expressions of Caspase-3, Caspase-8, Caspase-9, Bax and Bcl-2. Results　The results of the MTT colorimetric method showed that, with the control group as a reference, the growth inhibition rate of the PC-3 cells in each experimental group increased with the extension of the intervention time, and the group B, the group C and the group D showed obvious PC-3 cell growth inhibitory effect after 48-hour intervention. The results of PI fluorescence staining showed that apoptotic morphology appeared in the PC-3 cells after the intervention of Res, and the number of apoptotic bodies increased with the increase of the concentration of Res intervention. The results of flow cytometry showed that the apoptotic rates of the PC-3 cells in the group A, the group B, the group C and the group D were (1.35±0.65)%, (5.17±1.18)%, (10.74±2.24)% and (21.16±3.13)%, respectively, and the apoptotic rate of the PC-3 cells in the control group was (0.77±0.11)%, and the differences were statistically significant(F=64.332, P=0.000). The results of RT-qPCR showed that compared with those in the control group, the relative expressions of Caspase-3, Caspase-9, Caspase-8 and Bax mRNA in the group C and the group D were significantly increased(P<0.05), and the relative expression of Bcl-2 mRNA was significantly decreased(P<0.05). Conclusion　Res can inhibit the proliferation of human prostate cancer PC-3 cells and promote their apoptosis, which may be related to the regulation of the Caspase-3/Bax/Bcl-2 apoptosis signaling pathway.]]> 2022/3/8 10:33:08 LI Jia-xuan, CHEN Mei-ni, GUO Wei 471true ［Abstract］　Objective　To compare the effects of atorvastatin and rosuvastatin on recurrence rate and atrial electrical remodeling after radiofrequency ablation in patients with paroxysmal atrial fibrillation. Methods　One hundred and twenty patients with paroxysmal atrial fibrillation who underwent radiofrequency ablation in Department of Cardiology, Luoyang Central Hospital Affiliated to Zhengzhou University from January 2019 to June 2020 were selected and divided into the atorvastatin group, the rosuvastatin group and the non-statin treatment group by random number table method, with 40 cases in each group. All the three groups received oral omeprazole treatment for 1 month, warfarin or rivaroxaban treatment for at least 2 months, and amiodarone treatment for 3 months. On this basis, the atorvastatin group received atorvastatin treatment, 20 mg each time, once a day, for 3 months, and the rosuvastatin group received rosuvastatin treatment, 10 mg each time, once a day, for 3 months. The non-statin treatment group did not receive any statin. The recurrence rate of atrial fibrillation, P-wave dispersion of electrocardiogram and low-density lipoprotein level were compared among the three groups 6 months after operation. Results　Within 6 months after operation, atrial fibrillation recurred in 8 cases(20.00%) in the atorvastatin group, 10 cases(25.00%) in the rosuvastatin group, and 12 cases(30.00%) in the non-statin treatment group. There was no significant difference in the recurrence rate of atrial fibrillation among the three groups(χ²=1.067, P=0.587). Compared with that before operation, the P-wave dispersion of electrocardiogram in the atorvastatin group reduced significantly 6 months after operation(P<0.05). However, compared with that before operation, the P-wave dispersion of electrocardiogram in the rosuvastatin group and the non-statin treatment group was not significantly different 6 months after the operation(P>0.05). Compared with those before operation, the levels of low-density lipoprotein in the atorvastatin group and the resuvastatin group reduced significantly 6 months after operation(P<0.05). However, compared with that before operation, the level of low-desity lipoprotein in the non-statin treatment group was not significantly different 6 months after the operation(P<0.05). Conclusion　There is no significant difference between atorvastatin and rosuvastatin in the short-term recurrence rate of patients with paroxysmal atrial fibrillation after radiofrequency ablation, but the P-wave dispersion of electrocardiogram of the atorvastatin group decreases more significantly, suggesting that lipophilic atorvastatin is more conducive to improving atrial electrical remodeling.]]> 2022/3/8 10:33:08 GU Yun-fei, ZHANG Xuan, DUAN Ka-dan, et al. 470true ［Abstract］　Objective　To investigate the effect of erythrocyte cold agglutination on various detection parameters of blood cell analysis and the treatment methods. Methods　Seventy-three whole blood specimens with erythrocyte cold agglutination from December 2017 to February 2020 in Fujian Cancer Hospital were selected. The Sysmex XN-9000 automatic blood cell analyzer was used to detect the samples, and the differences in the result parameters of red blood cell(RBC), hematocrit(HCT), mean corpuscular volume(MCV), mean corpuscular hemoglobin(MCH), mean corpuscular hemoglobin concentration(MCHC), white blood cell(WBC), hemoglobin(Hb) and platelet(PLT) were compared before and after water bath at 37 ℃. Results　There were significant differences in the results of RBC, HCT, MCV, MCH and MCHC before and after water bath treatment(P<0.05), but there were no significant differences in the results of WBC, Hb and PLT(P>0.05). Conclusion　The results of RBC, HCT, MCV, MCH and MCHC indexes are distorted in the cold agglutination of specimens, while the results of WBC, Hb and PLT indexes are not affected by cold agglutination. During blood cell analysis, the inspectors should strengthen quality control, find the cold agglutinated specimens in time and treat them with water bath at 37℃ to eliminate interference.]]> 2022/3/8 10:33:08 GAO Yan-ni, CHEN Yan,CUI Zhao-lei, et al. 469true ［Abstract］　Objective　To analyze the expression levels of micro-ribonucleic acid-200b(miR-200b) and micro-ribonucleic acid-655(miR-655) in the serum and intestinal mucosal tissues of patients with ulcerative colitis(UC), and to explore their relationships with disease activity. Methods　Thirty-six UC patients admitted to Department of Gastroenterology, Nanxishan Hospital of Guangxi Zhuang Autonomous Region from October 2016 to June 2019 were selected. According to the improved Mayo scoring system, they were divided into the endoscopic active group(20 cases) and the endoscopic healing group(16 cases), and other 20 healthy volunteers during the same period were selected as the control group. Real-time quantitative polymerase chain reaction(RT-qPCR) method was used to detect the expression levels of miR-200b and miR-655 in intestinal mucosal tissues and serum samples, and these expression levels were compared among the three groups. Results　The expression levels of miR-200b and miR-655 in the intestinal mucosa of the control group were significantly higher than those of the endoscopic active group and the endoscopic healing group(P<0.05), while there was no statistically significant difference between the endoscopic active group and the endoscopic healing group(P>0.05). The serum levels of miR-200b and miR-655 in the endoscopic active group were higher than those in the endoscopic healing group and the control group, and the differences were statistically significant(P<0.05). However, there were no significant differences in the serum levels of miR-200b and miR-655 between the latter two groups(P>0.05). Conclusion　The expression levels of serum miR-200b and miR-655 may indirectly reflect the intestinal mucosal injury in UC patients.]]> 2022/3/8 10:33:08 LUO Wen-jie, ZHANG Qi-fang, ZHANG Hai-lian, et al. 468true ［Abstract］　Objective　To explore the application effect of controlled low central venous pressure(CLCVP) under goal-directed fluid therapy technique on spinal surgery. Methods　Fifty-nine patients who underwent spinal surgery(including posterior spinal canal decompression, intervertebral disc removal, intervertebral bone grafting and pedicle screw internal fixation) in Guangzhou Red Cross Hospital Affiliated to Jinan University from June 2020 to June 2021 were selected. The patients were divided into L group(receiving CLCVP fluid therapy, 20 cases), G group(receiving goal-directed fluid therapy, 19 cases) and LG group(receiving CLCVP under goal-oriented fluid therapy, 20 cases) by the random number table method. All the groups received combined intravenous inhalational anesthesia. The circulatory indicators［including mean arterial pressure(MAP), heart rate(HR), central venous pressure(CVP) and cardiac index(CI)］ and perfusion indicators［including central venous-arterial carbon dioxide partial pressure difference(P_cv-aCO₂), systemic central venous oxygen saturation(S_cvO₂) and arterial blood lactate(Lac)］ were compared among the three groups at the time of entry(T₁), after induction(T₂), before lumbar spinal canal decompression(T₃), at the completion of spinal canal decompression(T₄), and at the end of surgery(T₅). The operation time, duration of anesthesia, fluid input and fluid loss were compared among the three groups. The correlation between mean of CI(CI_m), mean of MAP(MAP_m), mean of CVP(CVP_m) and intraoperative blood loss was analyzed. Results　From T₁ to T₅ time points, HR showed an upward trend in the three groups, and the fluctuation of CI was not significant, and the range of the changes was not statistically significant among the three groups(P>0.05). At the T₂ and T₃ time points, the MAP and the CVP in the G group were significantly higher than those in the L group and the LG group, and the differences were statistically significant(P<0.05). From T₁ to T₅ time points, P_cv-aCO₂ showed a gradual upward trend in the L group, while a trend of rising first and then falling in the G group and the LG group, and the differences in the changes were statistically significant(P<0.05). From T₁ to T₅ time points, the Lac showed a gradual upward trend in the three groups, and the difference in the range of the changes was statistically significant among the three groups(P<0.05). At T₄ and T₅ time points, the Lac level in the L group was significantly higher than that in the G group and the LG group(P<0.05). From T₁ to T₅ time points, S_cvO₂ increased first and then decreased in the three groups, and there were no statistically significant differences in the changes(P>0.05). The input volume of crystal liquid, colloidal fluid, and total fluid input in the G group were significantly greater than those in the L group and the LG group(P<0.05), but there were no significant differences between the L group and the LG group(P>0.05). The intraoperative blood loss in the G group was greater than that in the L group, and the urine output in the G group was greater than that in the L group and the LG group, and the differences were statistically significant(P<0.05). Conclusion　CLCVP under goal-directed fluid therapy technique is beneficial to reduce intraoperative blood loss and improve perfusion in patients undergoing spinal surgery.]]> 2022/3/8 10:33:08 HUANG Hao-ran, CAO Yang, LIANG Bing 467true ［Abstract］　Objective　To observe the changes of corneal nerve fibers and the degree of inflammatory infiltration in patients with Sjgren′s syndrome(SS) by using in vivo confocal microscopy(IVCM), and to explore the correlation between them and dry eye symptoms. Methods　From January 2017 to October 2019, 22 SS patients who were admitted to Department of Ophthalmology, the People′s Hospital of Guangxi Zhuang Autonomous Region, were selected as the observation group, and 22 healthy volunteers during the same period were selected as the control group. The morphology of nerve fibers and Langerhans cell density in the central cornea were observed by using IVCM. The tear film break-up time(BUT), Schirmer′s test(ST), and tear meniscus height(TMH) results of the research subjects were detected and recorded, and their relationships with Ocular Surface Disease Index(OSDI) scale score were analyzed. Results　The detection results of BUT, ST and TMH indexes in the observation group were significantly lower than those in the control group(P<0.05), and the OSDI scale scores in the observation group were significantly higher than those in the control group(P<0.05). Compared with the control group, the observation group had thin subepithelial nerve fibers in the central cornea, increased number of beadings, and increased density of Langerhans cells, and the differences were statistically significant between the two groups(P<0.05). The results of Pearson correlation analysis showed that the OSDI scale scores of the observation group were significantly negatively correlated with BUT, ST and nerve fiber diameter(P<0.05), and were significantly positively correlated with Langerhans cell density(P<0.05), but were not significantly correlated with TMH and the number of nerve fiber beads(P>0.05). Conclusion　IVCM can be used to observe the morphology of corneal subepithelial nerve fibers and the degree of Langerhans cell infiltration. In SS patients, the diameter of corneal subepithelial nerve fibers becomes thin; the number of nerve fiber beads and the Langerhans cell density increase, and these changes are related to dry eye symptoms.]]> 2022/3/8 0:00:00 YANG Zhao, HE Wen-jing, CHEN Li-fei, et al. 466true ［Abstract］　Objective　To investigate the role of contrast-enhanced transesophageal echocardiography(TEE) in assessing left atrial appendage thrombus and function detection and application value in patients with atrial fibrillation before radiofrequency catheter ablation. Methods　Twenty patients with atrial fibrillation who sought medical advice in West China Hospital of Sichuan University from October 2020 to December 2020 and planned to undergo radiofrequency catheter ablation were selected, and all the patients underwent conventional TEE and contrast-enhanced TEE. The left atrial appendage function indexes(left atrial appendage emptying, filling velocity) and the detections of left atrial appendage thrombus in the two methods were assessed. Results　The indexes of left atrial appendage emptying and filling velocity obtained by contrast-enhanced TEE were faster than those obtained by conventional TEE, and the number of the patients with left atrial appendage emptying velocity ≤0.35 m/s detected by contrast-enhanced TEE was less than that detected by conventional TEE, and the differences were statistically significant(P<0.05). The conventional TEE examination showed suspicious thrombus and spontaneous imaging of the left atrial appendage in 5 cases. No obvious filling defects were found in the left atrial appendage after contrast-enhanced angiography. All the 20 patients underwent radiofrequency catheter ablation, and no obvious embolic events occurred during the operation, 3 days after the operation, and 3 months after the operation. Conclusion　Contrast-enhanced TEE examination can improve the accuracy of diagnosis of left atrial appendage thrombus.]]> 2022/3/8 10:33:08 LIU Zhi-yue, LI Qian, HAUNG He, et al. 465true ［Abstract］　Objective　To observe the efficacy of chiropractic and massage combined with acupuncture in treatment of lumbar disc herniation(LDH). Methods　Ninety LDH patients admitted to Zhongshan Hospital of Traditional Chinese Medicine from May 2018 to March 2021 were selected and divided into observation group and control group by random number table method, with 45 cases in each group. The control group was treated with chiropractic and massage, and the observation group was treated with chiropractic and massage combined with acupuncture, with a treatment course of four weeks for both groups. The clinical efficacy was compared between the two groups. The Japanese Orthopaedic Association(JOA) score, the Visual Analogue Scale(VAS) score, the quality of life score, and the values of average surface electromyography(AEMG) during the process of lumbar extension and lumbar flexion were compared between the two groups before and after treatment. Results　The total clinical effective rate of the observation group was higher than that of the control group, and the difference was statistically significant(95.56% vs 80.00%; χ²=5.075, P=0.024). After treatment, the JOA and SF-36 scores increased and the VAS scores decreased in the two groups. Compared with those in the same group before treatment, the scores were significantly different after treatment(P<0.05), and the observation group had more significant changes than the control group(P<0.05). After treatment, the AEMG values increased during the lumbar extension process and decreased during the lumbar flexion process in the two groups. Compared with those in the same group before treatment, the AEMG values were significantly different after treatment(P<0.05), and the observation group had more significant changes than the control group(P<0.05). Conclusion　Chiropractic and massage combined with acupuncture can promote pain relief, improve the mechanical effects of back muscles and lumbar spine function, improve the quality of life, and benefit the prognosis of the patients in the treatment of LDH.]]> 2022/3/8 10:33:08 LIANG Ning-jun 464true ［Abstract］　Objective　To investigate the efficacy and safety of AngioJet mechanical thrombus aspiration in treatment of middle/high-risk acute pulmonary embolism(APE). Methods　The clinical data of 18 middle/high-risk APE patients admitted to the Fourth Affiliated Hospital of Guangxi Medical University from January 2020 to March 2021 were selected. All the patients underwent AngioJet mechanical thrombus aspiration. The incidence of the patients′ intraoperative complications, postoperative clinical efficacy, and the changes in clinical indicators such as respiration, heart rate, transcutaneous oxygen saturation(TCSO₂), mean pulmonary artery pressure, and serum creatinine before and after surgery were analyzed. Results　The patients′ surgical success rate was 100.0%, and the clinical symptom improvement rate was 83.3%(15/18). The success rate of clinical treatment was 94.4%(17/18), and one case died after operation. The results of postoperative angiography showed complete recanalization of the embolic pulmonary artery in 7 cases, partial recanalization in 10 cases, and no recanalization in 1 case. Two cases of arrhythmia occurred during the operation. No pulmonary vascular injury such as pulmonary artery dissection and rupture occurred during the operation. The incidence of intraoperative bleeding was 27.8%(5/18), and minor bleeding occurred in all the cases of intraoperative bleeding. Compared with those before operation, the respiration, heart rate and mean pulmonary artery pressure of the 17 patients who were successfully treated were decreased, and the TCSO₂ was increased after operation, and the differences were statistically significant(P<0.05). Compared with those before treatment, serum creatinine levels did not change significantly after treatment(P>0.05). Conclusion　AngioJet mechanical thrombus aspiration is safe and effective in treatment of middle/high-risk APE. Standardized, gentle operation and rapid aspiration of thrombus are helpful to further improve the surgical safety and improve the prognosis of the patients.]]> 2022/3/8 10:33:08 WEI Li-chun, SU Yi-ming, XU Tai-fu, et al. 463true ［Abstract］　Objective　To explore the correlation of intraoperative regional cerebral oxygen saturation(rScO₂) and nerve injury markers with postoperative delirium(POD) in patients undergoing heart surgery. Methods　Sixty patients undergoing heart valve replacement surgery under cardiopulmonary bypass(CPB) in Henan Provincial Chest Hospital from January 2020 to June 2021 were selected, and 3 of them were excluded. The patients were divided into POD group(15 cases) and non-POD group(NPOD group, 42 cases) according to the occurrence of POD within 3 days after operation. During the operation, a near-infrared spectrometer was used to continuously monitor the patients′ rScO₂ and pulse oximetry(SpO₂). The difference in artery-rScO₂(da-rScO₂) was calculated. The patients′ central venous blood was drawn to determine the serum levels of nerve injury marker S100β protein and neuron-specific enolase(NSE) 10 minutes(min) before induction of anesthesia(T₀), 10 min after induction of anesthesia(T₁), immediately after CPB(T₂), at the end of CPB(T₃), after operation(T₄), when the patients were admitted to the intensive care unit(ICU)(T₅), and 1 day(T₆), 2 days(T₇) and 3 days(T₈) after operation, respectively, using enzyme-linked immunosorbent assay(ELISA) method. The above observation data were compared between the two groups, and the correlation between these observation data and the occurrence of POD was analyzed. Results　There were no significant differences between the two groups in age, weight, height, gender, etiology, comorbidities, history of smoking, history of drinking, as well as basic blood oxygen saturation and baseline value of rScO₂(P>0.05). Compared with the NPOD group, the POD group had longer aortic occlusion time, shorter cerebral perfusion time and CPB time, but the differences were not statistically significant(P>0.05). The duration of intraoperative reduction of rScO₂>25% in the POD group was significantly longer than that in the NPOD group(P<0.05). During CPB, the minimum mean arterial pressure and the minimum rScO₂ in the POD group were lower than those in the NPOD group, while the maximum da-rScO₂ and the duration of da-rScO₂>50%, the duration of da-rScO₂>40%, the duration of rScO₂<50% and the duration of rScO₂<40% in the POD group were significantly greater than those in the NPOD group, and the differences were statistically significant(P<0.05). From T₀ to T₈, the levels of serum nerve injury markers S100β and NSE in the two groups showed an upward trend, and the increase in the POD group was greater. From T₂ to T₈ time points, the levels of serum S100β and NSE in the POD group were higher than those in the NPOD group, and the differences were statistically significant(P<0.05). The results of multivariate logistic regression analysis showed that the longer duration of rScO₂ reduction>25%, the duration of rScO₂<40% and the duration of da-rScO₂>50% during CPB, and higher levels of serum S100β and NSE were the risk factors promoting the occurrence of POD(P<0.05). Conclusion　During CPB, longer duration of rScO₂ reduction>25%, duration of rScO₂<40%, and duration of da-rScO₂>50% are risk factors for promoting the occurrence of POD in patients undergoing heart surgery under CPB, and the elevated levels of nerve injury markers S100β and NSE are also associated with the occurrence of POD.]]> 2022/1/29 11:54:58 ZHOU Jun-hui, GAO Jie, MENG Xian-hui 462true ［Abstract］　Objective　To analyze the expression levels of melanoma-associated antigen family member D4(MAGE-D4) and actin-binding protein CORO1C mRNA in glioma, and to explore their correlation and clinical significances. Methods　The data of six hundred and eighty-one glioma samples［518 cases of low-grade glioma(LGG) and 163 cases of glioblastoma(GBM)］ and the data of 207 cases of normal brain tissue samples were collected through Gene Expression Profiling Interactive Analysis(GEPIA) database, and the expression levels of MAGE-D4 and CORO1C mRNA were compared among them. The clinical samples of 62 cases of glioma tissues and 11 cases of normal brain tissues were further collected, and reverse transcription-quantitative polymerase chain reaction(RT-qPCR) was used to detect the expression levels of MAGE-D4 and CORO1C mRNA, and the expression levels of MAGE-D4 and CORO1C mRNA were compared between the two clinical samples. The correlation between the expression levels of MAGE-D4 and CORO1C mRNA and the clinical indicators of the glioma patients was analyzed. Small interfering RNA(siRNA) technology was used to silence the expression of glioma cell line MAGE-D4, and the changes in the expression of CORO1C mRNA was detected. Results　The results of GEPIA database data analysis showed that the expression levels of MAGE-D4 and CORO1C mRNA in the GBM and LGG tissues were higher than those in the normal brain tissues, and the differences were statistically significant(P<0.05). The RT-qPCR detection results of the clinical samples also showed that the expression levels of MAGE-D4 and CORO1C mRNA in the glioma tissues were significantly higher than those in the normal brain tissues(P<0.05). The expression levels of MAGE-D4 and CORO1C mRNA were not significantly correlated with the patient′s age, gender, World Health Organization(WHO) grade, pathological type, Karnofsky performance score(KPS), the longest diameter of the tumor, and the expression levels of Ki-67, glial fibrillary acidic protein(GFAP), S-100 and Vimentin(P>0.05). The results of Pearson correlation analysis showed that there was a positive correlation between the expression levels of MAGE-D4 and CORO1C mRNA in the clinical glioma sample tissues(r=0. 848, P=0.000). The results of cell experiment showed that the expression levels of CORO1C mRNA in U87 MG and U251 cells decreased significantly after the U87 MG and U251 cells were silenced by MAGE-D4 siRNA(P<0.05). Conclusion　MAGE-D4 and CORO1C mRNA are highly expressed in glioma tissues, and their expression levels are positively correlated. MAGE-D4 and CORO1C may synergistically affect the occurrence and development of glioma, and they have the potential to become targets for molecular targeted therapy of glioma.]]> 2022/1/29 11:54:58 ZHANG Yuan, ZHANG Peng-bo, XIE Huan, et al. 461true ［Abstract］　Objective　To screen methylation markers in gene promoter region of colorectal adenoma using genome-scale methylation chip. Methods　Nine patients with advanced colorectal adenoma(adenoma group) who were hospitalized in the People′s Hospital of Guangxi Zhuang Autonomous Region and the First Affiliated Hospital of Guangxi Medical University from January 2013 to December 2014 were selected. In addition, 20 patients whose inflammatory bowel disease, precancerous lesions and cancer were excluded by colonoscopy were selected as the control group. The colorectal adenoma specimens(diameter ≥2 cm) in the adenoma group were resected under endoscopy, and the colorectal mucosa specimens were taken in the control group(13 cases of colonic mucosa, 7 cases of rectal mucosa). The tissue DNA was extracted and genome-scale methylation chip was used for methylation detection, and the differential methylation markers in gene promoter region were screened by bioinformatics analysis. Results　According to Δβ≥|0.4|, a total of 1 870 differential methylation markers were found in the gene promoter region, among which 1 524 were hypermethylation markers, and 346 were hypomethylation markers, including 929 differential promoter methylation genes. The results of GO analysis showed that the differentially methylated genes of promoters were mainly enriched in annotation items such as chemical synaptic transmission, nervous system development, extracellular matrix tissue, extracellular matrix structural components, RNA polymerase Ⅱ regulatory region sequence-specific DNA binding, and sequence-specific DNA binding, plasma membrane, proteinaceous extracellular matrix and cell membrane. KEGG_Pathyway analysis showed that promoter differential methylation genes were mainly involved in signal transduction pathways such as neural activity ligand-receptor interaction, nicotine addiction, calcium signaling pathway, etc. Conclusion　Screening the methylation markers of colorectal adenomas using genome-scale methylation chip is helpful to explore the pathogenesis of colorectal adenomas and provide clues for the early diagnosis of the disease.]]> 2022/1/29 11:54:58 HE Chun-gang, HUANG Qin-yuan, ZHONG Shi-biao, et al. 460true ［Abstract］　Objective　To explore the efficacy of speckle-tracking echocardiography(STE) combined with plasma miR-30a in evaluating the short-term prognosis of patients with acute coronary syndrome(ACS), and to provide a reference for guiding clinical treatment. Methods　Two hundred and fifty-two ACS patients who were admitted to the Sixth Affiliated Hospital of South China University of Technology from January 2020 to December 2020 were selected, and all of them underwent complete revascularization of the diseased coronary artery. According to the prognosis 1 month after the onset of the disease, the patients were divided into a good prognosis group(199 cases) and a poor prognosis group(53 cases). STE was used to detect left ventricular global longitudinal strain(LV-GLS) and left ventricular ejection fraction(LVEF). Real-time polymerase chain reaction(PCR) method was used to detect the level of plasma miR-30a. Multivariate logistic regression was used to analyze the impact of LV-GLS and plasma miR-30a indicators on the short-term prognosis of ACS patients, and the receiver operating characteristic(ROC) curve method was used to analyze the efficacy of LV-GLS and plasma miR-30a indicators in evaluating the short-term prognosis of ACS patients. Results　The LV-GLS of the good prognosis group was lower than that of the poor prognosis group, and the relative expression of plasma miR-30a in the good prognosis group was lower than that in the poor prognosis group, and the differences were statistically significant(P<0.05). However, there was no significant difference in LVEF indicator between the two groups(P>0.05). The results of Pearson correlation analysis showed that there was a positive correlation between LV-GLS and plasma miR-30a levels in ACS patients(r=0.418, P=0.000). The results of multivariate logistic regression analysis showed that higher levels of LV-GLS(OR=1.415) and plasma miR-30a(OR=2.201) were the risk factors for the short-term poor prognosis of ACS(P<0.05). The results of ROC curve analysis showed that both LV-GLS and plasma miR-30a had the application value of evaluating the short-term prognosis of ACS patients(AUC=0.921, AUC=0.825; P<0.05), and the combination of the two indicators could improve the evaluation efficacy(AUC=0.949, P<0.05). Conclusion　STE combined with plasma miR-30a detection can be used to assess the short-term prognosis of patients with ACS. The combined detection can provide a reference for clinical treatment, and is worthy of promotion and application.]]> 2022/1/29 11:54:58 ZHAO Juan, CHAI Ren-jie, SONG Shi-bo, et al. 459true ［Abstract］　Objective　To summarize the diagnosis and treatment mothods and prognosis of post-hepatectomy portal vein thrombosis(PH-PVT) in 16 cases. Methods　The clinical data of 16 patients with PH-PVT admitted to Beijing Tsinghua Changgung Hospital from February 2015 to February 2021 were retrospectively analyzed, and their clinical characteristics and diagnosis and treatment process were summarized. All the 16 cases were confirmed by enhanced CT examination. Among the 16 cases, there were 7 males and 9 females, aged 34-74(58.44±11.29)years old, including 4 cases of hepatocellular carcinoma(HCC), 8 cases of perihilar cholangiocarcinoma(PHCC), 1 case of cholangiocarcinoma, 1 case of gallbladder cancer(GBca) invading the liver, 1 case of intrahepatic bile duct papilloma and 1 case of intrahepatic bile duct stones. Results　Among the 16 cases, except for 2 patients who were diagnosed with chronic PH-PVT on day 144 and day 351 after hepatectomy, the other 14 patients had the time of diagnosis of PH-PVT from day 1 to day 24 after the operation, with an average of 9th day. After the formation of PVT, 4 patients had no clinical symptoms; 9 patients had unexplained fever; 4 patients had abdominal pain and nausea; 3 patients had abdominal distension, hematemesis, blood in the stool and jaundice symptoms, and 5 patients eventually developed hepatic encephalopathy and liver failure. After anticoagulant, thrombolytic and surgical treatments, 8 cases were improved and discharged, and 8 cases died. Conclusion　There are many risk factors for PH-PVT.PH-PVT can be diagnosed by enhanced CT, and its treatment methods include anticoagulation, thrombolysis and surgical thrombus removal. At present, there is no clear method to predict and prevent PH-PVT, and early detection and early treatment are the keys to improving the prognosis of the patients.]]> 2022/1/29 11:54:58 CHEN Bao-jun, TONG Xuan, FAN Hai-ning, et al. 458true ［Abstract］　Objective　To explore the application effect of transesophageal echocardiography(TEE) on anesthesia for cardiac surgery under cardiopulmonary bypass(CPB). Methods　Thirty-five patients with heart disease who underwent CPB cardiac surgery at Zhaoqing Second People′s Hospital from February 2019 to February 2021 were selected. Among them, there were 12 cases receiving aortic valve replacement, 10 cases receiving mitral valve replacement+aortic valve replacement, and 13 cases receiving mitral valve replacement. TEE monitoring was performed before and during operation to evaluate the effect of the operation, and the gas volume classification was observed after heart resuscitation and before CPB shutdown. The changes in lactic acid and mean arterial pressure(MAP) levels of the patients before operation(T₀), intraoperative 10 min(T₁), intraoperative 30 min(T₂), and intraoperative 60 min(T₃) were observed. The stroke volume(SV), fraction of area change(FAC), cardiac index(CI), and aortic velocity time integral(AVTI) of the patients were compared before and after operation. Results　The valve leaflets of the 35 patients were able to move normally after operation, and no paravalvular leakage was found. The average time from heart resuscitation to CPB shutdown was (25.26±5.01)min, and no complications occurred. In the 35 patients, 27 patients had different degrees of gas in left atrium after heart resuscitation, including 12 cases of grade 1, 10 cases of grade 2, and 5 cases of grade 3. Before CPB shutdown, only 6 cases were classified as grade 1. There were no significant changes in the levels of lactic acid and MAP in the patients at the time points of T₀, T₁, T₂ and T₃(P>0.05). Compared with those before operation, the levels of CI, AVTI, FAC, and SV of the patients increased after operation, and the differences were statistically significant(P<0.05). Conclusion　TEE monitoring during CPB cardiac surgery anesthesia can accurately evaluate the valve conditions and surgical effects in patients with heart disease, and has important guiding significance in terms of intracardiac exhaust and CPB shutdown.]]> 2022/1/29 11:54:58 CHE Long-tong, LI Zhen-xing, ZHOU Shao-yu, et al. 457true ［Abstract］　Objective　To explore the clinical application effect of cardiopulmonary combined with ultrasound target-directed fluid management on the intensive care unit(ICU) patients after cardiac surgery. Methods　Fifty patients undergoing cardiac surgery who were admitted to the ICU of Liuzhou Worker′s Hospital from June 2020 to March 2021 were selected and divided into a study group and a control group by random number table method, with 25 cases in each group. Cardiopulmonary combined with ultrasound target-directed fluid management was used to guide infusion for the patients in the study group, while central venous pressure was used to guide infusion for the patients in the control group. The levels of heart rate, respiration rate, mean arterial pressure(MAP), oxygenation index(PaO₂/FiO₂), blood lactic acid, and left ventricular ejection fraction were compared between the two groups 24 hours after surgery, as well as other clinical indicators such as the dosage of adrenaline and dopamine, total infusion volume and blood transfusion volume in patients within 24 hours after surgery. The second operation rate and 28-day mortality rate were compared between the two groups. Results　The respiratory rate and the heart rate in the study group were lower than those in the control group 24 hours after surgery, and the levels of MAP and PaO₂/FiO₂ in the study group were higher than those in the control group, and the differences were statistically significant(P<0.05). Within 24 hours after surgery, the dosage of adrenaline and dopamine, total infusion volume and blood transfusion volume in the study group were lower than those in the control group, and the differences were statistically significant(P<0.05). The use time of ventilator, ICU stay and hospitalization time in the study group was shorter than that in the control group 24 hours after surgery, and the difference was statistically significant(P<0.05). One case in the study group needed a second operation, and 2 cases in the control group needed a second operation, and there was no significant difference in the second operation rate between the two groups(χ²=0.000, P=1.000). One case died in the control group 28 days after operation and 0 cases died in the study group, and there was no significant difference in the postoperative 28-day mortality rate between the two groups(χ²=0.000, P =1.000). Conclusion　Cardiopulmonary combined with ultrasound target-directed fluid management has a good application effect on ICU patients after cardiac surgery, and it is worthy of clinical recommendation.]]> 2022/1/29 11:54:58 LI Xia, LAI Jun-hua, MA Hua-yi 456true ［Abstract］　Objective　To compare the efficacy of minimally invasive and open Sweet esophagectomy in treatment of Siewert type Ⅱ adenocarcinoma of the esophagogastric junction(AEG). Methods　The clinical data of 137 AEG patients admitted to Anhui Provincial Hospital Affiliated to Anhui Medical University from March 2015 to September 2017 were selected, and the patients were divided into the minimally invasive Sweet esophagectomy(MISE) group(38 cases) and the open Sweet esophagectomy(OSE) group(99 cases) according to the different surgical methods. The intraoperative indicators and postoperative curative effect were compared between the two groups. Results　Compared with the OSE group, the MISE group had less intraoperative blood loss, longer operation time, and more total number of lymph node dissections and number of abdominal lymph node dissections, and the differences were statistically significant(P<0.05). A total of 45 patients(32.85%) in the two groups had postoperative complications of grade 2 and above, and lung infection was the most common complication. The major serious complications of grade 4 and above were mainly severe anastomotic leakage and respiratory failure. The incidence of grade 2-3 complications in the OSE group was higher than that in the MISE group, and the differences were statistically significant(P<0.05). The 1-year survival rates of the MISE group and the OSE group were 92.1% and 87.9%, respectively, and the 3-year survival rates of the MISE group and the OSE group were 68.2% and 59.6%, respectively. There was no significant difference in postoperative survival between the two groups(P=0.383). There were no significant differences in postoperative recurrence and metastasis between the two groups(P>0.05). The results of Cox regression analysis showed that with N₀ stage as the reference, N₃ stage was an independent adverse factor affecting the patients′ survival time(P<0.05). Conclusion　Compared with the OSE procedure, the MISE procedure fails to improve the survival prognosis of the patients with Siewert type Ⅱ AEG. However, the patients receiving MISE procedure have less intraoperative blood loss and less postoperative complications, which is more conducive to rapid recovery.]]> 2022/1/29 11:54:58 LI Huan, MEI Xin-yu 455true ［Abstract］　Objective　To observe the efficacy of probiotics in treatment of refractory primary immune thrombocytopenia(ITP) and their effects on the changes of intestinal flora. Methods　Twenty patients with refractory ITP were randomly divided into probiotics group and control group, with 10 cases in each group. The probiotics group was given danazol+cyclosporine+Bifidobacterium Lactobacillus triple live bacteria tablets orally. The control group was given danazol+cyclosporine+vitamin C tablets orally. Blood samples were collected from the patients, and the levels of tumor necrosis factor-α(TNF-α)、interleukin-12(IL-12) and interferon-γ(IFN-γ) were determined by quantitative enzyme-linked immunosorbent assay(ELISA) method before treatment and 8 weeks after treatment. Fecal samples were collected, and the abundance of Bifidobacterium, Lactobacillus, Coprococcus and Escherichia coli was analyzed by 16SrDNA sequencing, and the intestinal microecology was analyzed. Results　After treatment, the platelet count in the probiotics group was significantly higher than that in the control group(P<0.05); the ratio of Firmicutes/Bacteroidetes in the probiotics group was significantly higher than that in the control group(P<0.05); the abundance of Lactobacillus and the abundance of Bifidobacterium in the probiotics group were significantly higher than those in the control group(P<0.05); the abundance of Escherichia coli in the probiotics group was significantly lower than that in the control group(P<0.05). After treatment, there was no significant difference in the abundance of Coprococcus between the two groups(P>0.05). The levels of TNF-α, IL-12 and IFN-γ in the probiotics group were significantly lower than those in the control group(P<0.05). Conclusion　There is intestinal flora imbalance in refractory ITP. Oral treatment with probiotics is conducive to improve the prognosis of the patients.]]> 2022/1/29 11:54:58 HUANG Xiao-chun, QIN Li-juan, MO Qiu-yu 454true ［Abstract］　Objective　To explore the analgesic and anti-inflammatory effects of Bagui Analgesic Ointment(BAO) on mice. Methods　Kunming female mice were selected as the experimental subjects, and they were divided into blank control group(no drug intervention), Voltaren group(receiving Voltaren ointment intervention) and BAO group(receiving BAO intervention) according to different intervention methods. The analgesic and anti-inflammatory effects of the intervention treatments on mice were evaluated by the hot plate experiment, the acetic-acid-induced twisting experiment and the xylene-induced auricle swelling experiment in mice. Results　From 15 minutes to 45 minutes after administration, the pain threshold of Voltaren group showed a gradually increasing trend, while the pain threshold of BAO group showed an increasing trend at first and then a decreasing trend. The pain thresholds of the two groups at each time point after administration were higher than those of the blank control group, and the differences were statistically significant(P<0.05). At the time point of 45 minutes after administration, the pain threshold of BAO group was lower than that of the Voltaren group, and the difference was statistically significant(P<0.05). Both the Voltaren group and the BAO group had lower number of body twisting than the blank control group, and the differences were statistically significant(P<0.05). There were no significant differences in the number of body twisting and the inhibition rate of body twisting between the Voltaren group and the BAO group(P>0.05). The mass of the swollen auricles in the Voltaren group was smaller than that in the BAO group and the blank control group, and the mass of the swollen auricles in the BAO group was also smaller than that in the blank control group, and the differences were statistically significant(P<0.05). Conclusion　BAO has better analgesic and anti-inflammatory effects, and its effects are equivalent to those of Voltaren ointment.]]> 2022/1/29 11:54:58 ZHAO Li-juan, ZHANG Yan-ju, ZHANG Cheng-hao, et al. 453true ［Abstract］　Objective　To investigate the protective effects and mechanisms of down-regulated expression of miR-27a on alleviating acute lung injury(ALI) after myocardial ischemia-reperfusion(MI/R) in rats. Methods　Forty adult male Sprague-Dawley(SD) rats aged 8-12 weeks were randomly divided into sham operation group(SP group), model group(I/R group), miR-27a inhibitor group(anta-27a+I/R group) and miR-27a inhibitor blank control group(NC+I/R group), with 10 rats in each group. The rat model of ALI caused by MI/R was established by ligation of the left anterior descending branch of the coronary artery for 30 minutes and then reperfusion for 120 minutes. Antagomir-27a and antagomir-NC were injected through tail vein in the anta-27a+I/R group and the NC+I/R group for 3 consecutive days before modeling. The wet/dry weight ratio(W/D) and pathological changes of the lung were compared among the four groups. The expression levels of malondialdehyde(MDA) and superoxide dismutase(SOD) in the lung tissues were compared among the four groups. The expression levels of interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) in the lung tissues and serum were compared among the four groups. Results　Compared with those in the SP group, the lung tissues of rats in the I/R group,the anta-27a+I/R group and the NC+I/R group were seriously injured, and the lung W/D value was increased, and the level of MDA in the lung tissues was increased, and the level of SOD in the lung tissues was decreased, and the levels of IL-6 and TNF-α in the lung tissues and serum were increased, and the differences were statistically significant(P<0.05). Compared with those in the I/R group, the degree of pathological injury of the lung tissues of rats in the anta-27a+I/R group was alleviated, and the lung W/D value was decreased, and the level of MDA in the lung tissues was decreased and the level of SOD in the lung tissues was increased, and the levels of IL-6 and TNF-α in the lung tissues and serum were decreased, and the differences were statistically significant(P<0.05). Conclusion　Down-regulation of miR-27a expression can alleviate ALI induced by MI/R in rats by reducing inflammatory response and oxidative stress.]]> 2022/12/30 11:06:44 GU Wen-yu, XU Gui-ping, CHEN Zhe, et al. 452true ［Abstract］　Objective　To investigate the expression of hsa_circ_0021350 in glioma and its clinical significance, and to preliminarily evaluate its potential application in the diagnosis and treatment of glioma. Methods　Forty glioma tissue specimens(including 22 cases of high-grade gliomas and 18 cases of low-grade gliomas) and 10 normal brain tissue specimens were collected in Department of Neurosurgery, the First Affiliated Hospital of Guangxi Medical University, from January 2016 to May 2022. The ring-like properties of hsa_circ_0021350 were verified by RNase R digestion combined with reverse transcription-polymerase chain reaction(RT-PCR). The amplification products of quantitative real-time PCR(qRT-PCR) were identified by TA clone sequencing. The expression levels of hsa_circ_0021350 were compared among the high-grade gliomas, the low-grade gliomas and the normal brain tissues. The correlation between hsa_circ_0021350 expression and clinicopathological indicators was analyzed in the glioma patients. Results　The divergent primers designed in this study could effectively and specifically amplify hsa_circ_0021350, and hsa_circ_0021350 was ring-shaped and tolerant to RNase R digestion. The experimental results of qRT-PCR showed that the expression levels of hsa_circ_0021350 in the high-grade gliomas and the low-grade gliomas were higher than those in the normal brain tissues, and the expression level of hsa_circ_0021350 in the high-grade gliomas was higher than that in the low-grade gliomas, and the differences were statistically significant(P<0.05). The expression of hsa_circ_0021350 was significantly correlated with the grade of glioma and the positive rate of ki67(P<0.05). Conclusion　Hsa_circ_0021350 is highly expressed in gliomas, especially in high-grade gliomas, and is related to tumor proliferation factor ki67. hsa_circ_0021350 can be used as a potential molecular target for diagnosis and treatment of gliomas.]]> 2022/12/30 0:00:00 ZOU Xiao-qiong, XUE Chun-hong, WANG Ping, et al. 451true ［Abstract］　Objective　To explore the effect and mechanisms of icariin(ICA) to optimize rat adipose-derived stem cells(rASCs) in promoting the survival of ultra-long random skin flaps. Methods　Six-week-old female healthy specific-pathogen-free(SPF) Sprague-Dawley(SD) rats were selected as the experimental animals. The rASCs were extracted and their multilineage differentiation ability and surface markers were identified. Western blot(WB) was used to detect the protein expressions of angiogenesis factors［vascular endothelial growth factor(VEGF), platelet-derived growth factor-BB(PDGF-BB), fibroblast growth factor-2(FGF-2), insulin-like growth factor-2(IGF-2)］ in the rASCs stimulated by ICA at different concentrations(0, 0.1, 1, 10 μM). The rASCs group(untreated rASCs), the ICA+rASCs group(rASCs pretreated with 1 μM ICA), and the blank control group(an equal volume of PBS) were set up, with 3 rats in each group. According to the grouping, the corresponding cell suspensions were injected into the pedicle of the flaps, respectively. The survival rates of the flaps were evaluated 7 days, 14 days and 28 days after treatment. The survival tissues of the flaps were dissected to detect the microvessel density(MVD), collagen content(%) and the protein expressions of angiogenesis factors in each group 28 days after treatment by histological and WB experiments. Results　The rASCs had the ability of adipogenesis, osteogenesis, and cartilage differentiation. The rASCs had high expressions of CD29 and CD44, and no expression of CD45. The results of WB experiment suggested that 1-10 μM ICA could significantly improve the expressions of angiogenesis factors in rASCs(P<0.05). The results of the animal experiments showed that the flap survival area ratios in the rASCs group and the ICA+rASCs group were elevated 7 days and 14 days after treatment compared with those in the blank control group(P<0.05). However, only the flap survival area rate in the ICA+rASCs group was significantly higher than that in the blank control group 28 days after treatment(P<0.05). The results of the histological experiments showed that the rASCs group and ICA+rASCs group had a more tightly arranged flap structure, higher MVD and collagen content than the blank control group(P<0.05). The results of the WB experiments suggested that the protein expression levels of VEGF, PDGF-BB, and FGF-2 in the rASCs group and the ICA+rASCs group were significantly higher than those in the blank control group(P<0.05), and the protein expression levels of VEGF and PDGF-BB in the ICA+rASCs group were higher than those in the rASCs group, and the differences were statistically significant(P<0.05). Conclusion　ICA can elevate the expression levels of angiogenesis factors in rASCs, which may be the mechanism of its optimization of rASCs to promote the survival of ultra-long random skin flaps in rat models.]]> 2022/12/30 11:06:44 LIANG Zhi-jie, HUANG Dong-lin, ZHU Dan-dan, et al. 450true ［Abstract］　Objective　To investigate the correlation between the expression of centromeric protein L(CENPL) and the recurrence of hepatocellular carcinoma after liver transplantation. Methods　A total of 93 patients with recurrent hepatocellular carcinoma who underwent liver transplantation in the Third People′s Hospital Affiliated to Fujian University of Traditional Chinese Medicine, the 900th Hospital of the Joint Logistic Support Force of Chinese People′s Liberation Army and the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2014 to June 2020 were selected as the recurrence group. Forty-six patients with hepatocellular carcinoma who underwent liver transplantation at the same time but did not relapse after operation were selected as the non-recurrence group. Immunohistochemical staining method was used to observe the expressions of CENPL in hepatocellular carcinoma tissues and their adjacent tissues. The correlation between the expression of CENPL and the clinicopathological indicators and survival prognosis was analyzed. Results　Microscopically, CENPL staining was positive in the cancer tissues, mainly expressed in the nucleus, and a small amount of CENPL was expressed in the cytoplasm. The results of multivariate Cox regression analysis showed that China Liver Cancer Staging(CNLC) stage Ⅰ was an inhibitory factor for HCC recurrence after liver transplantation with CNLC stage Ⅱ as the reference［HR(95%CI)=0.46(0.13-0.91), P=0.013］, and the high expression of CENPL was a promoting factor for HCC recurrence after liver transplantation with the low expression of CENPL as the reference［HR(95%CI)=1.68(1.41-2.99), P=0.003］. The median recurrence-free survival(RFS) of the high CENPL expression group was 5(3-22)months, and the median RFS of the low CENPL expression group was 8(5-26)months, and the difference was statistically significant between the two groups(log-rank test: χ²=9.269, P=0.002). The median overall survival(OS) of the high CENPL expression group was 14(8-40)months, and the median OS of the low CENPL expression group was 22(9-37)months, and the difference was statistically significant between the two groups(log-rank test: χ²=4.406, P=0.031). Conclusion　CENPL has predictive value for HCC recurrence after liver transplantation, which is worthy of further study.]]> 2022/12/30 11:06:44 FANG Jian, RUAN Mei, XIA Lei, et al. 449true ［Abstract］　Objective　To evaluate the application effects of different doses of sufentanil combined with ropivacaine on labor analgesia and their influences on maternal and infant outcomes. Methods　Two hundred and ten parturients who underwent painless delivery in Tangshan Maternal and Child Health Hospital from January 2017 to August 2020 were selected. They were divided into group A, group B and group C by random number table method, with 70 cases in each group. Because 14 cases were converted to cesarean section, 196 cases were finally included, including 65 cases in the group A, 66 cases in the group B and 65 cases in the group C. The analgesic regimens of the three groups were different: the group A, sufentanil 0.3 μg/ml combined with 0.1% ropivacaine; the group B, sufentanil 0.4 μg/ml combined with 0.1% ropivacaine; the group C, sufentanil 0.5 μg/ml combined with 0.1% ropivacaine. The Visual Analogue Scale(VAS) scores were compared among the three groups before labor analgesia(T₀), 30 min after labor analgesia(T₁), at the time when the uterus opened to the full extent(T₂) and 2 hours after delivery(T₃). The modified Bromage scores were compared among the three groups 1 hour after delivery. The course of labor and the maternal and neonatal outcomes were compared among the three groups. Results　The first stage of labor in the group C was significantly longer than that in the group A and the group B(P<0.05). There were no significant differences in the second and third stages of labor among the three groups(P>0.05). There were no significant differences in the utilization rate of forceps, the rate of lateral episiotomy, the incidence of birth canal laceration and the incidence of postpartum hemorrhage among the three groups(P>0.05). At the time points from T₀ to T₃, the VAS scores showed a downward trend in the three groups, and the VAS scores in the group B and the group C showed a more significant downward trend than those in the group A(P<0.05). At the time points from T₁ to T₃, the VAS scores in the group C were lower than those in the group B and the group C, and the differences were statistically significant(P<0.05). There were no significant differences in the modified Bromage scores among the three groups(P>0.05). There were no significant differences in birth weight, Apgar scores at the first minute, the fifth minute and the tenth minute, umbilical artery blood pH value, umbilical artery blood partial pressure of carbon dioxide(PCO₂) and umbilical artery blood partial pressure of oxygen(PO₂) among the three groups(P>0.05). Conclusion　0.3 μg/ml, 0.4 μg/ml, 0.5 μg/ml sufentanil combined with 0.1% ropivacaine can be safely and effectively applied in labor analgesia, among which 0.4 μg/ml sufentanil combined with 0.1% ropivacaine analgesia has little effect on labor and is recommended for clinical use.]]> 2022/12/30 11:06:44 LIU Guo-dong, JIA Yu-gang, QIANG Rui-hua, et al. 448true ［Abstract］　Objective　To investigate the effects of Ghrelin on oxidative stress and inflammatory response in the hippocampus of mice with perioperative neurocognitive disorder(PND) after surgery. Methods　Specific-pathogen-free(SPF) healthy male C57BL/6J mice were selected as the experimental animals. Through water maze positioning and navigation training, the learning ability of mice was tested, and the qualified mice were selected and randomly divided into Sham group, PND group, PND+Vehicle group and PND+Ghrelin group, with 12 mice in each group. The mice in the Sham group were anesthetized by intraperitoneal injection of 5% chloral hydrate, and the skin on the tibia side of the calf was incised and sutured. No surgery or treatment was performed, and lidocaine mucilage was administered to relieve pain after the operation. The mice in the PND group were anesthetized by intraperitoneal injection of 5% chloral hydrate and then underwent intramedullary fixation of tibial fractures. After the operation, lidocaine mucilage was administered for postoperative analgesia, and no other therapeutic interventions were required. The mice in the PND+Ghrelin group underwent intramedullary fixation for tibial fractures, and the operation method was the same as that of the PND group. Ghrelin(80 μg/kg) was intraperitoneally injected 2 hours before anesthesia and after recovery, once a day for 7 days. The mice in the PND+Vehicle group were intraperitoneally injected with 0.9% saline(1.6 ml/kg) 2 hours before anesthesia and after recovery, once a day for 7 consecutive days. The other interventions were the same as those in the PND+Ghrelin group. On the 7th day after the operation, the memory ability of the mice in the four groups was tested by the water maze space exploration experiment. The enzyme-linked immunosorbent assay(ELISA) method was used to detect the serum levels of cyclooxygenase-2(COX-2), high-mobility group box-1(HMGB1). The levels of malondialdehyde(MDA), total superoxide dismutase(T-SOD), COX-2 and HMGB1 in the hippocampus were detected by using ELISA method and Western blot method, respectively. Results　The escape latency was longer in the PND group, the PND+Vehicle group and the PND+Ghrelin group than that in the Sham group, and the number of escape platform entries within 60 s, and the proportion of the target quadrant stay time in the PND group, the PND+Vehicle group and the PND+Ghrelin group were lower than those in the Sham group, and the differences were statistically significant(P<0.05). The escape latency in the PND+Ghrelin group was shorter than that in the PND group, and the number of escape platform entries within 60 s and the proportion of the target quadrant stay time in the PND+Ghrelin group were higher than those in the PND group, and the differences were statistically significant(P<0.05). Compared with those in the Sham group, the levels of COX-2 and HMGB1 in the serum and hippocampus in the PND group, the PND+Vehicle group, and the PND+Ghrelin group were increased, and the differences were statistically significant(P<0.05). Compared with those in the PND group, the levels of COX-2 and HMGB1 in the serum and hippocampus in the PND+Ghrelin group were decreased, and the differences were statistically significant(P<0.05). Compared with those in the Sham group, the levels of MDA in the hippocampus in the PND group, the PND+Vehicle group, and the PND+Ghrelin group were increased, and the levels of T-SOD in the PND group, the PND+Vehicle group, and the PND+Ghrelin group were decreased, and the differences were statistically significant(P<0.05). The level of MDA in the hippocampus tissues in the PND+Ghrelin group was lower than that in the PND group, and the level of T-SOD in the hippocampus tissues in the PND+Ghrelin group was higher than that in the PND group, and the differences were statistically significant(P<0.05). Conclusion　Ghrelin has a protective effect on PND mice, and its mechanism may be related to the improvement of postoperative inflammation and oxidative stress.]]> 2022/12/30 11:06:44 ZHAN Qiao-zhi, YAN Wen-jun 447true ［Abstract］　Objective　To explore the clinical application value of in-vivo confocal microscopy(IVCM) in evaluating the morphological changes of corneal nerves in patients with type 2 diabetes. Methods　One hundred and eighty patients with type 2 diabetes diagnosed from December 2017 to December 2019 were selected, and divided into non-diabetic retinopathy(NDR) group(90 cases, 90 eyes) and diabetic retinopathy(DR) group(90 cases, 90 eyes) according to whether they were complicated with diabetic retinopathy. Ninety healthy volunteers(90 eyes) during the same period were selected as the control group. All the research subjects underwent IVCM examination, and the central corneal nerve fiber density(CNFD), corneal nerve branch density(CNBD) and corneal nerve fiber length(CNFL) were compared among the three groups. Results　The IVCM examination showed that the number and branches of corneal nerve fibers in the NDR group and the DR group were less than those in the control group, and the course of corneal nerve fibers was stiff. The levels of CNFD, CNBD and CNFL in the NDR group and the DR group were significantly lower than those in the control group(P<0.05). The levels of CNFD, CNBD and CNFL in the DR group were significantly lower than those in the NDR group(P<0.05). Conclusion　IVCM examination can quickly, non-invasively and accurately detect the early changes of corneal nerve fibers in diabetic patients, and can provide objective scientific basis for the prevention, early diagnosis and early intervention of DR.]]> 2022/12/30 11:06:44 CHEN Li-fei, HUANG Hui, SHEN Chao-lan, et al. 446true ［Abstract］　Objective　To observe the curative effect of percutaneous reduction and screw fixation on treatment of displaced intra-articular calcaneal fractures. Methods　Twenty-two cases(25 affected feet) of displaced intra-articular calcaneal fractures who were treated with percutaneous reduction and screw fixation in Department of Orthopedics, the Affiliated Hospital of Qingdao Binhai University from August 2020 to October 2021 were selected. Among the patients, there were 19 males(22 affected feet) and 3 females(3 affected feet), and their age ranged from 29 to 71(46.82±7.59)years. The causes of the injuries: fall accident from high places(20 affected feet) and traffic accident(5 affected feet). Sanders classification: 13 affected feet of type Ⅱ and 12 affected feet of type Ⅲ. The time from injury to operation was 3-12(7.71±2.73)days. The operation time, intraoperative blood loss, wound healing and postoperative complications were recorded. The length, width and height of the affected heel bone, as well as the changes of Böhler angle and Gissane angle before operation, on the second day after operation and at the final follow-up were analyzed. The Visual Analogue Scale(VAS) score, American Orthopaedic Foot and Ankle Society(AOFAS) ankle-hindfoot score, and Maryland score were used to evaluate the recovery of the patients. Results　The operation was successfully completed on the 22 patients(25 affected feet) in this group. The operation time was (57.15±5.42)min, and the intraoperative blood loss was (13.02±1.76)ml. All the incisions healed by first intention. There were no serious complications such as incision infection, nonunion of fracture, malunion, and neurovascular injury. The Böhler angle, Gissane angle, and the length, height and width of the calcaneus were significantly improved after operation compared with those before the operation(P<0.05). The preoperative VAS scores of the patients were (7.04±0.20)points, which decreased to (4.30±0.20)points on the second day after the operation, and decreased to (1.20±0.20)points at the final follow-up. At the final follow-up, the AOFAS ankle-hindfoot scores were (91.40±6.50)points, and the Maryland scores were (93.40±4.90)points. The scoring results were excellent in 16 feet and good in 9 feet, and the excellent and good rate was 100.00%. Conclusion　Percutaneous reduction and screw fixation is minimally invasive, safe and highly satisfactory in the treatment of calcaneal fractures for the patients. However, it is difficult to obtain anatomic reduction of the articular surface for complex fractures with collapsed articular surface, and further improvement is required.]]> 2022/12/30 0:00:00 FANG Bing, LIU Jian-quan, LI Zhi-shuai 445true ［Abstract］　Objective　To investigate the application value of hepatitis C virus core antigen(HCV-cAg) detection in early diagnosis of hepatitis C infection. Methods　Ninety patients with HCV-cAg positive and 164 patients with hepatitis C virus antibody(HCV-Ab) positive who received treatment in the Affiliated Hospital of Southwest Medical University from January 2020 to July 2021 were selected as the research subjects. The levels of glutamic-pyruvic transaminase(ALT) and hepatitis C virus ribonucleic acid(HCV-RNA) in the patients′ serum were detected, and nucleic acid test results were used as the gold standard to analyze the effectiveness of HCV-cAg detection in the early diagnosis of hepatitis C infection. Results　Among the 90 cases of HCV-cAg positive specimens, 76 cases were positive for HCV-RNA, with the positive rate being 84.44%, and 54 cases were abnormal liver function, with the abnormal rate being 60.00%. Among the 164 cases of HCV-Ab positive, 114 cases(69.51%) were HCV-RNA positive, and 75 cases(45.73%) had abnormal live function. The rate of positive HCV-RNA and abnormal liver function in the HCV-cAg positive group were significantly higher than those in the HCV-Ab positive group(P<0.05), and the sample′s optical density/cut-off(S/CO) value of HCV-cAg was associated with abnormal liver function(P<0.05). Conclusion　The detection results of HCV-cAg can reflect the liver function status, and can be used for early screening and efficacy evaluation of hepatitis C.]]> 2022/12/30 11:06:44 JIANG Ling, LI Sheng, WANG Zhi-yao, et al. 444true ［Abstract］　Objective　To compare the clinical efficacy of AngioJet mechanical thrombus aspiration and catheter thrombectomy combined with thrombolysis in treatment of intermediate/high-risk acute pulmonary embolism(APE). Methods　Thirty-six patients with intermediate/high-risk APE who were admitted to the Fourth Affiliated Hospital of Guangxi Medical University from January 2019 to June 2022 were selected. The patients were divided into thrombus aspiration group(12 cases, treated with AngioJet mechanical thrombus aspiration) and thrombolysis group(24 cases, treated with catheter thrombectomy combined with thrombolysis) according to their treatment intentions. The technical success rate, treatment success rate, operation time, urokinase dosage, incidence of adverse events, and recurrence rate of pulmonary embolism(PE) were compared between the two groups. Results　Compared with the thrombolysis group, the thrombus aspiration group had longer operation time, less amount of urokinase and greater difference in the percutaneous arterial oxygen saturation(SpO₂) before and after operation, and the difference was statistically significant(P<0.05). There were no significant differences in the technical success rate and the treatment success rate between the two groups(91.67% vs 95.83%, 75.00% vs 79.17%; P>0.05). There were no significant differences in the incidence rates of pulmonary vascular injury, renal function injury and arrhythmia between the two groups(P>0.05). There was no bleeding in the thrombus aspiration group, while bleeding occurred in 8 cases(33.33%) in the thrombolysis group, and the difference in the bleeding rate between the two groups was statistically significant(P<0.05). After 3-24(12.50±3.25)months of follow-up, there was no significant difference in the recurrence rate of PE between the two groups(16.67% vs 12.50%; P>0.05). Conclusion　Both AngioJet mechanical thrombus aspiration and catheter thrombectomy combined with thrombolysis are effective and minimally invasive therapies for treatment of intermediate/high-risk APE. For patients with bleeding tendency, AngioJet mechanical thrombus aspiration has more advantages in safety.]]> 2022/12/30 11:06:44 WEI Li-chun, SU Yi-ming, XU Tai-fu, et al. 443true ［Abstract］　Objective　To investigate the clinical characteristics and treatment methods for patients with autoimmune hemolytic anemia(AIHA) secondary to thalassemia intermedia or β-thalassemia major(abbreviation for β-thalassemia). Methods　The clinical characteristics of 42 patients with AIHA secondary to thalassemia intermedia or β-thalassemia major who were admitted to Department of Hematology, the 923rd Hospital of the Joint Logistics Support Force of Chinese People′s Liberation Army from January 2009 to December 2020 were retrospectively analyzed. The effects of different treatment regimens were compared. Results　The main manifestations of the 42 patients were a larger-than-expected decrease in hemoglobin, shorter interval between blood transfusions, and positive anti-human globulin test. Thirty-two patients received hormone therapy, including 20 patients in the standard therapy group and 12 patients in the pulse therapy group. The complete response rate (75.0% vs 83.3%) and the effective rate (91.7% vs 95.0%) were not significantly different between the two groups(P>0.05). One case was relieved after first-line splenectomy. Nine patients received no treatment, and 3 of them had spontaneous complete remission. Three patients who did not respond to hormone therapy or relapsed were relieved after splenectomy. Conclusion　The anti-human globulin test should be performed on the patients with β-thalassemia when the hemolysis is aggravated and the blood transfusion interval is shortened in them. The patients diagnosed with AIHA should be treated with glucocorticoid in time. Splenectomy can be considered for patients with refractory or recurrent thalassemia.]]> 2022/11/30 11:57:24 LIAO Gui-ping, LI Jing, ZHOU Tian-hong, et al. 442true ［Abstract］　Objective　To observe the clinical efficacy of percutaneous spinal endoscopy “isolation zone” technique in treatment of discogenic low back pain. Methods　Forty-five patients with discogenic low back pain admitted to Cangzhou Central Hospital from September 2017 to September 2020 were selected. The patients were treated with percutaneous spinal endoscopic surgery via lateral approach under local anesthesia. Endoscopic removal of nucleus pulposus, annulus fibrosis forming, denervation of ligamentum flavum and decompression of the corresponding segmental nerve roots throughout the process, forming an “isolation zone” around the nerve root without contact with the nucleus pulposus and annulus fibrosis were performed. Visual Analogue Scale(VAS) scores and Oswestry Disability Index(ODI) scores were recorded before surgery and 1, 3, 6, and 12 months after surgery, and the clinical efficacy were evaluated using modified MacNab criteria at the last follow-up. Results　The operations on the 45 cases were successfully completed, and the operation time was 65-125(94.7±17.7)minutes. One case had a spinal cord hypertensive reaction during the operation, and the symptom disappeared 30 minutes after the removal of water pressure and oxygen inhalation. One case had symptoms of femoral nerve palsy after operation, and was treated conservatively with neurotrophy, acupuncture and moxibustion physiotherapy and functional exercise, and the symptoms disappeared 4 weeks after operation. There were no serious complications such as permanent nerve damage and intervertebral space infection in the patients. The VAS scores and ODI scores of the pain in the lower back and lumbosacral region after operation were significantly improved compared with those before operation(P<0.05). According to the modified MacNab criteria, at the last follow-up, the curative effect was excellent in 24 cases, good in 13 cases, and fair in 8 cases, with an excellent and good rate of 82.2%. Conclusion　The percutaneous spinal endoscopy “isolation zone” technique is safe and effective in treatment of discogenic low back pain, and is worthy of clinical promotion.]]> 2022/11/30 11:57:24 WANG Lu, LI Ling-xia, CHENG Cai, et al. 441true ［Abstract］　Objective　To systematically evaluate the effect of ABCB1 genetic polymorphism on the blood concentration of therapeutic drug imatinib in chronic myeloid leukemia and gastrointestinal stromal tumor patients, and to provide evidence-based basis for guiding clinical individualized medication. Methods　PubMed, EMBASE, Web of Sciences, Scopus, China National Knowledge Internet(CNKI) and Wanfang databases were retrieved. The studies on the correlations between ABCB1 C1236T, G2677T/A and C3435T gene polymorphisms of imatinib pharmacokinetic pathway transporters and the blood concentrations in patients treated with imatinib were searched. Literature screening and data extraction were conducted according to the inclusion and exclusion criteria. Meta-analysis was performed by using Review Manager 5.4. Results　A total of 7 studies on the association between ABCB1 gene polymorphism and the blood concentration of imatinib were included(eight hundred and seventy-three patients in total). The results of meta-analysis showed statistically significant difference in the steady-state blood concentration of imatinib between the carriers of T allele and the carriers of C allele in ABCB1 C1236T(WMD=97.44 ng/m, P=0.03), and between the homozygotes+heterozygotes carriers and wild-type carriers in G2677T/A(WMD=318.27 ng/ml, P<0.01), and no significant association was found between C3435T gene polymorphism and the blood concentration of imatinib(P>0.05). Conclusion　The ABCB1 C1236T and G2677T/A mutated genes are associated with the elevated blood concentration of imatinib.]]> 2022/11/30 11:57:24 YIN Ji-yuan, XIA Yan-zhe, CHEN Jie, et al. 440true ［Abstract］　Objective　To analyse the factors affecting postoperative survival and recurrence in patients with intermediate and high-risk gastrointestinal stromal tumors(GIST). Methods　The clinical data of 370 GIST patients admitted to Department of Gastrointestinal Surgery, the First Affiliated Hospital of Nanchang University from January 2012 to December 2021 were retrospectively analyzed. According to the modified National Institutes of Health(NIH) risk classification criteria, there were 255 high-risk GIST patients and 115 intermediate-risk GIST patients. The recurrence-free survival(RFS) and overall survival(OS) of patients undergoing laparoscopic surgery and laparotomy, as well as patients after R0 resection and R1 resection were compared. Cox regression was used to analyze the factors affecting postoperative survival and recurrence in patients with intermediate and high-risk GIST. Results　The immunohistochemical results showed that the positive rate of CD117 was 99.46%(368/370), and the positive rate of CD34 was 77.03%(285/370). The most common primary sites were stomach(48.92%, 181/370) and small intestine(37.30%, 138/370). The RFS of the laparoscopic surgery patients was significantly better than that of the laparotomy patients(P<0.05), but there was no significant difference in OS between the two groups(P>0.05). The RFS of the patients after R0 resection was significantly better than that of the patients after R1 resection(P<0.05), but there was no significant difference in OS between the two groups(P>0.05). The results of multivariate Cox regression analysis showed that risk classification, GIST cell morphology, and targeted drug therapy were independent factors affecting OS of the patients(P<0.05); age, risk classification, degree of radical surgery, Ki-67 index, mitoses, GIST cell morphology and targeted drug therapy were independent factors for RFS in intermediate and high-risk GIST patients(P<0.05). For 302 intermediate and high-risk GIST patients who received targeted drug therapy, the RFS of the patients with targeted therapy duration >3 years was significantly better than that of the patients with duration of medication <1 year and that of the patients with duration of medication from 1 to 3 years, and the differences were statistically significant(P= 0.023). However, there was no significant difference in OS among the three groups(P>0.05). Conclusion　Risk classification, GIST cell morphology, and receiving targeted drug therapy are factors that affect the prognosis of patients with intermediate and high-risk GIST. Prolonging the time of targeted drug therapy is beneficial for better prognosis of the patients.]]> 2022/11/30 11:57:24 JU Hou-qiong, ZHONG Chong-han, LIU Dong-ning, et al. 439true ［Abstract］　Objective　To investigate the value of conventional ultrasound combined with contrast-enhanced ultrasound(CEUS) in the diagnosis of thyroid nodules, and to analyze the characteristics and causes of misdiagnosed nodules in the combined diagnosis. Methods　Four hundred and eighty-one patients with thyroid nodules(522 nodules in total) who were admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2019 to June 2021 were selected. All the patients received conventional ultrasound examination first, and then received conventional ultrasound combined with CEUS examination. Among the 481 patients, there were 130 males and 351 females, aged from 19 to 71 years, with an average of (37.2±15.3)years, and the largest diameter of the lesions was 0.5-3.9(1.8±0.7)cm. The diagnostic efficacy of conventional ultrasound and combined CEUS in benign and malignant thyroid nodules was compared, and the characteristics of the combined diagnosis in the misdiagnosis of thyroid nodules were analyzed. Results　Of the 522 nodules, 203 were confirmed as malignant nodules and 319 as benign nodules by pathological examination. A total of 59 cases of TI-RADS classification and diagnosis of the nodules were revised by conventional ultrasound combined with CEUS, and 6 cases were incorrectly revised. Fifty-three nodules were misdiagnosed by the combined examination, among which 41 benign nodules were misdiagnosed as malignant nodules, and 12 malignant nodules were misdiagnosed as benign nodules. The benign nodules misdiagnosed as malignant nodules all contained two or more than two of the following conventional ultrasound features: hypoechoic, aspect ratio ≥1, ill-defined borders, irregular shape, capsular invasion and microcalcifications. The malignant nodules misdiagnosed as benign nodules showed CEUS features such as isoenhancement, hyperenhancement and ring enhancement. The Kappa consistency analysis test showed that the results of the conventional ultrasound and the conventional ultrasound combined with CEUS were consistent with the results of pathological examinations(P<0.05). However, the specificity, sensitivity and accuracy of the conventional ultrasound combined with CEUS in the differential diagnosis of benign and malignant thyroid nodules were higher than those of the conventional ultrasound(87.15% vs 81.19%; 94.09% vs 84.73%; 89.85% vs 82.57%). Conclusion　Conventional ultrasound combined with CEUS can improve the diagnostic value of thyroid nodules, but benign and malignant nodules have a certain degree of overlap in conventional ultrasound and CEUS features, which may lead to misdiagnosis.]]> 2022/11/30 11:57:24 WEI Liu, LING Bing, ZHENG Hong-yu, et al. 438true ［Abstract］　Objective　To analyse the risk factors of lower extremity deep vein thrombosis(DVT) in patients with renal malignancies after surgery and to construct a predictive model. Methods　The clinical data of 342 patients with renal malignancies who received surgical treatment in First People′s Hospital of Jinan from January 2016 to December 2021 were retrospectively analyzed. According to the occurrence of lower extremity DVT during hospitalization, the patients were divided into DVT group(26 cases) and non-DVT group(316 cases). Multivariate logistic regression was used to analyze the risk factors of lower extremity DVT in patients with renal malignancies after surgery, and the risk factors were screened out for constructing a predictive model and drawing a nomogram. Results　The age of the DVT group was older than that of the non-DVT group, and the proportion of women in the DVT group was greater than that in the non-DVT group, and the differences were statistically significant(P<0.05). There were no significant differences in the body mass index(BMI) level and the proportion of patients with smoking history, diabetes history, coronary heart disease history and hypertension history between the two groups(P>0.05). Compared with the non-DVT group, the DVT group had a higher proportion of tumor-type papillary carcinoma and chromophobe carcinoma, a greater proportion of radical nephroureterectomy, and longer operation time, and the differences were statistically significant(P<0.05). The levels of D-dimmer(D-D) and hypersensitive C-reactive protein(HsCRP) in the DVT group were higher than those in the non-DVT group, and the differences were statistically significant(P<0.05). The results of multivariate logistic regression analysis showed that female gender, older age, longer operation time, higher levels of D-D and HsCRP were the independent risk factors for lower extremity DVT in patients with renal malignancies after surgery(P<0.05). The area under the receiver operator characteristic(ROC) curve of the predictive model was 0.855. Conclusion　Age, gender, operation time, D-D and HsCRP are associated with DVT in patients with renal malignancies after surgery. The predictive model constructed in this study has good accuracy and can provide a reference for the prevention of DVT after renal malignancies.]]> 2022/11/30 11:57:24 LI Jing, SUN Yan 437true ［Abstract］　Objective　To compare the clinical efficacy of decitabine combined with venetoclax and decitabine combined with CAG regimen in treatment of elderly patients with newly treated acute myeloid leukemia(AML). Methods　Twenty-nine elderly patients with newly treated AML who were not suitable for intensive chemotherapy and were admitted to Jingzhou Hospital Affiliated to Yangtze University from May 2016 to February 2022 were selected. According to the patients′ willingness to choose the treatment plans, they were divided into venetoclax group(decitabine+venetoclax, 17 cases) and CAG group(decitabine+CAG regimen, 12 cases). The clinical efficacy and prognosis were compared between the two groups. Results　The patients were followed up for 8.1-70.3 months, with a median follow-up time of 27.7 months. By the end of the follow-up, 8 of the 29 patients survived, and these survivors were in the venetoclax group, of whom 5 patients were still in remission and received consolidation therapy; 3 patients relapsed after remission. Nineteen patients died(including 9 patients in the venetoclax group and 10 patients in the CAG group), and 2 patients were lost to follow-up. The complete remission(CR) rate, morphological complete remission with incomplete blood count recovery(CRi) rate, composite complete remission(CR+Cri) rate and overall response rate(ORR) in the venetoclax group were higher than those in the CAG group, but the differences were not statistically significant(P>0.05). The median overall survival(OS) of the venetoclax group was 10.73 months, and the median OS of the CAG group was 8.57 months, and there was no significant difference in survival prognosis between the two groups(P>0.05). The median progression-free survival(PFS) of the venetoclax group was 7.93 months, and the median PFS of the CAG group was 5.77 months, and there was no significant difference in recurrence between the two groups(P>0.05). Grade 3-4 hematologic adverse reactions occurred in 27 of 29 patients(16 cases in the venetoclax group and 11 cases in the CAG group). The median time of agranulocytosis was 24 days and 25 days in the venetoclax group and the CAG group, respectively, and the median platelet recovery time was 20 days and 18 days in the venetoclax group and the CAG group, respectively, with no significant differences between the two groups(P>0.05). There were no significant differences in the incidence rates of infections and bleeding between the two groups(P>0.05). Conclusion　Both decitabine combined with venetoclax and decitabine combined with CAG regimen have good clinical efficacy and good safety in elderly patients with newly treated AML.]]> 2022/11/30 11:57:24 ZHANG Shi-jia, XU Xuan-xuan, TANG Yuan-yan 436true ［Abstract］　Objective　To explore the potential mechanisms of Spatholobus Suberectus Dunn in treatment of aplastic anemia(AA) by using network pharmacology. Methods　The active components and target genes of Spatholobus Suberectus Dunn were obtained through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP), and the relevant target genes of AA were retrieved through the GeneCards database, and the Venn diagram was used to analyze their intersection. GO functional enrichment analysis and KEGG pathway enrichment analysis were performed on key targets by using R software. The drug-active ingredient-key target-pathway-disease network was constructed by using Cytoscape 3.8.2 software. Results　Twenty-four active ingredients were obtained through TCMSP screening, and their 56 corresponding target genes were identified. One thousand six hundred and sixty-five disease target genes of AA were retrieved from the GeneCards database. The results of Venn diagram analysis showed that there were 21 intersecting target genes. Based on the 21 key target genes, a total of 1 052 enrichment results were obtained by GO functional enrichment analysis, including 1 012 biological processes(BP), 13 cellular components(CC), and 27 molecular functions(MF). A total of 70 signaling pathways were obtained by KEGG pathway enrichment analysis, and the main pathways included apoptosis, p53 signaling pathway, AGE-RAGE signaling pathway in diabetic complications, and disease-related pathways such as viruses, cancer, and infectious diseases. Conclusion　Spatholobus Suberectus Dunn plays a role in the treatment of AA through the interaction of multiple components, multiple targets and multiple pathways, which provides a reference for further exploring the mechanisms of Spatholobus Suberectus Dunn in the treatment of AA.]]> 2022/11/30 11:57:24 TAN Hong-di, LU Yi, WAN Yan-ni, et al. 435true ［Abstract］　Objective　To analyze the efficacy of apparent diffusion coefficient(ADC) value and ADC ratio(rADC) of magnetic resonance in differential diagnosis of low-risk and intermediate-high-risk prostate cancer. Methods　Sixty-two prostate cancer patients admitted to the Seventh People′s Hospital of Zhengzhou from January 2018 to December 2021 were selected, all of whom were confirmed by pathological examination. According to different Gleason scores, the patients were divided into intermediate-high-risk group(Gleason scores ≥7 points, 37 cases) and low-risk group(Gleason scores ≤6 points, 25 cases). The ADC values of cancer foci were measured in all the patients, and the corresponding rADC was calculated with reference to the contralateral side of the cancer foci, bladder urine and gluteus maximus. Spearman rank correlation analysis was used to explore the relationship between ADC value, rADC and Gleason grade of the cancer foci. Receiver operating characteristic(ROC) curve was used to evaluate the diagnostic efficacy of ADC value and rADC in differentiating low-risk prostate cancer from intermediate-high-risk prostate cancer. Results　Compared with those of the low-risk group, the ADC value and rADC of the intermediate-high-risk group were low, and the differences were statistically significant(P<0.05). The results of Spearman rank correlation analysis showed that the ADC value of the prostate cancer patients was negatively correlated with Gleason grade(r_s=-0.639, P=0.000). The rADC on the contralateral side of the cancer foci(rADC_{contralateral side of the cancer foci}), the rADC of the bladder(rADC_bladder), and the rADC of gluteus maximus(rADC_{gluteus maximus}) were all negatively correlated with Gleason grade(r_s=-0.578, P=0.000; r_s=-0.640, P=0.000; r_s=-0.603, P=0.000). The results of ROC curve analysis showed that ADC value, rADC_{contralateral side of the cancer foci}, rADC_bladder, and rADC_{gluteus maximus} could all be used to differentiate low-risk prostate cancer from intermediate-high-risk prostate cancer(P<0.05), and rADC_bladder had the highest diagnostic efficacy［area under the curve(AUC)=0.815］. Conclusion　The ADC value and rADC can be used to differentiate low-risk prostate cancer from intermediate-high-risk prostate cancer, and help to evaluate the biological characteristics of prostate cancer before surgery.]]> 2022/11/30 11:57:24 NIE Yang-ping, YUAN Shuai, LI Yun 434true ［Abstract］　Objective　To analyze the clinical features of colorectal juvenile polyp(JP) in children and the efficacy of endoscopic mucosal resection(EMR) in the treatment of JP. Methods　The clinical data of 40 children with JP admitted to Liuzhou Maternity and Child Healthcare Hospital from January 2017 to May 2021 were retrospectively analyzed. Among the 40 children, there were 31 males and 9 females. The age of the 40 children ranged from 7 months to 12 years, with a median age of 5 years. All the patients had a single colorectal JP. The clinical features and therapeutic prognosis of the patients were summarized. Results　Among the 40 cases, the growth site of JP was in the rectum in 23 cases(57.50%), in the sigmoid colon in 12 cases(30.00%), in the descending colon in 3 cases(7.50%), in the transverse colon in 1 case(2.50%), and in the ascending colon in 1 case(2.50%). There were 2 cases(5.00%) with diameter of polyps ≤0.5 cm, 17 cases(42.50%) with diameter of polyps from 0.6 cm to 1.0 cm, 15 cases(37.50%) with diameter of polyps from 1.1 cm to 2.0 cm, and 6 cases(15.00%) with diameter of polyps >2.0 cm. The minimum diameter was 0.5 cm and the maximum diameter was 5.0 cm. Yamada classification was type Ⅳ in 19 cases(47.50%), type Ⅲ in 11 cases(27.50%), type Ⅱ in 9 cases(22.50%), and type Ⅰ in 1 case(2.50%). Among the 40 specimens, 17 cases(42.50%) had chronic inflammatory changes in their specimens; 18 cases(45.00%) had small abscesses in their specimens, and no atypical hyperplasia and cancerous polyps were found. None of the pediatric patients in this group had serious complications such as infection, enterobrosis and massive hemorrhage, except for 2 cases who occasionally had a small amount of hemafecia within 10 days after surgery, and all the clinical symptoms disappeared 2 months later. The hemoglobin of 9 pediatric patients with mild anemia returned to normal 2 months after surgery. Twenty-two cases underwent reexamination with colonoscopy after surgery, and no recurrence was found. Conclusion　Colorectal juvenile polyps in children are most likely to occur in the rectum and sigmoid colon. EMR plus metal clip has high safety and good efficacy in the treatment of JP, which is worthy of clinical application.]]> 2022/11/30 11:57:24 QIN Yi, KONG Hong-xiang, YE Hui-lan, et al. 433true ［Abstract］　Objective　To investigate the expressions of hypoxia-inducible factor 1A(HIF1A) and melanoma-associated antigen D4(MAGED4) in human glioma, and to analyze the effect of HIF1A on the expression of MAGED4. Methods　Gene Expression Profiling Interactive Analysis(GEPIA) database was used to collect the data from 681 human glioma samples and the data from 207 normal brain tissue samples to compare the expression levels of MAGED4 mRNA and HIF1A mRNA. The mRNA-seq_693 data set and the corresponding clinical information of 693 glioma patients were downloaded from Chinese Glioma Genome Atlas(CGGA) database. The correlation between the expressions of MAGED4 mRNA and HIF1A mRNA, and the correlation between the two expressions and the clinical characteristics of the patients were analyzed. The hypoxic environment in vitro was simulated by using cobalt chloride, and the effect of silencing HIF1A expression in glioma cells on the expression of MAGED4 was investigated by reverse transcription-quantitative real-time polymerase chain reaction(RT-qPCR) and Western blot(WB) experiments. Results　The analysis results based on the GEPIA database showed that the levels of HIF1A mRNA and MAGED4 mRNA in the low-grade glioma(LGG) and the glioblastoma(GBM) tissues were higher than those in the normal brain tissues, and the differences were statistically significant(P<0.05). The analysis results based on the CGGA database showed that the MAGED4 mRNA expression level was positively correlated with the HIF1A mRNA expression level(r=0.322, P=0.000). The proportion of the patients with age<40 years and isocitrate dehydrogenase(IDH) mutation in the high MAGED4 mRNA expression group was greater than that in the low MAGED4 mRNA expression group, and the difference was statistically significant(P<0.05). There were no significant differences in gender, tumor type, World Health Organization(WHO) tumor grade, O6-methylguanie-DNA methyltransferase(MGMT) promoter methylation status and 1p/19q co-deletion between the two groups(P>0.05). U87-MG and U251 cells were transfected with HIF1A siRNA and were cultured for 48 hours under the condition of hypoxia. The results of RT-qPCR and WB experiments showed that the expression level of MAGED4 also decreased significantly after HIF1A was down-regulated. Conclusion　Both HIF1A and MAGED4 are highly expressed in gliomas, and their expressions are positively correlated. HIF1A can regulate the expression of MAGED4 in glioma cells.]]> 2022/11/2 11:21:28 WANG Ping, BI Shui-qing, LIU Chang, et al. 432true ［Abstract］　Objective　To analyze the predictive role of D-dimer level detection on the occurrence of post-thrombotic syndrome(PTS) before treatment of lower extremity deep vein thrombosis(DVT). Methods　The clinical data of 262 patients with lower extremity DVT who were admitted to the Department of Vascular Surgery of Taizhou People′s Hospital Affiliated to Nanjing Medical University from January 2016 to December 2020 were retrospectively analyzed. All the patients were treated with standard thrombolysis and anticoagulation. The patients were followed up for the occurrence of PTS within 1 year after discharge from the hospital, and the clinical data were compared between the PTS group and the non-PTS group. Receiver operator characteristic(ROC) curve was used to analyze the efficacy of D-dimer level before treatment in predicting the occurrence of PTS in the lower extremity DVT patients. Multivariate logistic regression model was used to analyze the risk factors of PTS in lower extremity DVT patients. Results　Seventy-five patients of the 262 lower extremity DVT patients developed PTS within 1 year after discharge from the hospital, with an incidence rate of 28.63%. Compared with the non-PTS group, the PTS group had high levels of body mass index(BMI) and D-dimer before treatment, and the PTS group had a great proportion of patients with lower extremity DVT duration ≥14 days, a great proportion of patients complicated with varicose veins and iliofemoral vein thrombosis, and the differences were statistically significant(P<0.05). The results of ROC curve analysis showed that the level of D-dimer before treatment could effectively predict the occurrence of PTS in patients with lower extremity DVT, and the area under the curve was 0.830(95%CI: 0.769～0.888; P=0.000) and the cut-off value was 3.645 mg/L. The results of multivariate logistic regression analysis showed that BMI≥24 kg/m²(OR=2.46), iliofemoral vein thrombosis(OR=2.16) and D-dimer level ≥3.645 mg/L before treatment(OR=2.92) were the independent risk factors for PTS in the lower extremity DVT patients(P<0.05). Conclusion　The determination of D-dimer level before treatment in patients with lower extremity DVT can help to identify the patients at high risk of PTS, which should arouse the attention of clinicians.]]> 2022/11/2 11:21:28 LI Cai-ying, ZHANG Yu, ZHAO Ying-hai, et al. 431true ［Abstract］　Objective　To investigate the effects of perioperative use of esketamine on the levels of serum neurological injury markers and the incidence of postoperative delirium(POD) in elderly patients after esophageal cancer radical surgery. Methods　Eighty elderly patients who underwent esophageal cancer radical surgery in Henan Provincial Chest Hospital from February 2021 to December 2021 were selected and divided into esketamine group(group E) and opioid group(group O) by random number table method, with 40 cases in each group. At the beginning of anesthesia induction, the group E was given intravenous injection of esketamine 0.5 mg/kg. During the maintenance of anesthesia, the infusing rate of esketamine was 0.5 mg/(kg·h), and the patients were stopped infusing 30 minutes before the operation, and immediately after the operation, patient controlled intravenous analgesia(PCIA) pump was set, and esketamine 1.0 mg/kg+sufentanil 50 μg+butorphanol 12 mg+tropisetron 10 mg was added to the analgesic pump, and diluted to 100 ml with normal saline. In the group O, sufentanil was injected intravenously with 0.2-0.4 μg/kg. During the maintenance of anesthesia, the remifentanil was administered at an infusing rate of 0.1-0.3 μg/(kg·min) and the patients were stopped infusing 5 minutes before the operation, and sufentanil was added to the PCIA pump, and fentanyl 100 μg+butorphanol 12 mg+tropisetron 10 mg was diluted to 100 ml with normal saline. The levels of serum nerve injury marker S100β protein and neuron-specific enolase(NSE) were compared between the two groups 10 minutes before induction of anesthesia, at the end of surgery, and on the 1st, 2nd, and 3rd day after surgery. The levels of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and interleukin-8(IL-8) were compared between the two groups on the 1st day before surgery and on the 1st and 3rd day after surgery. The incidence of POD within 3 days after surgery was compared between the two groups. Results　The incidence of POD within 3 days after surgery in the group E was lower than that in the group O, and the difference was statistically significant(12.50% vs 32.50%; χ²=4.588, P=0.032). Compared with those in the group O, the patients in the group E had less intraoperative propofol dosage and shorter post-anesthesia recovery unit(PACU) stay time, and the differences were statistically significant(P<0.05). During the observation period, the levels of S100β protein and NSE in the two groups showed an upward trend, and the levels of TNF-α, IL-6, and IL-8 showed a trend of first increasing and then decreasing, and the changes in the group E were smaller than those in the group O, and the differences were statistically significant(P<0.05). Conclusion　Perioperative use of esketamine can reduce the levels of S100β protein and NSE and the incidence of POD in elderly patients after esophageal cancer radical surgery, and the mechanisms may be related to the anti-inflammatory effect of esketamine.]]> 2022/11/2 11:21:28 WANG Ni, MA Hong-xia, ZHOU Jun-hui 430true ［Abstract］　Objective　To compare the clinical effects between two-time treatment and single treatment with 577 nm wavelength micropulse laser on severe diabetic macular edema(DME). Methods　Sixty severe DME patients(60 eyes) diagnosed and treated in the People′s Hospital of Guangxi Zhuang Autonomous Region from December 2015 to December 2019 were selected. They were divided into observation group and control group by random number table method, with 30 cases(30 eyes) in each group. The patients in both groups were treated with 577 nm wavelength micropulse laser first. The observation group received two-time treatment with micropulse laser(the second micropulse photocoagulation therapy was performed 6 weeks after the first treatment of micropulse laser), and the control group received only one-time treatment with micropulse laser. The changes of best corrected visual acuity(BCVA) and central macular thickness(CMT) were compared between the two groups before treatment, 6 weeks after treatment and 12 weeks after treatment. Results　After treatment, CMT showed a downward trend in both groups, and the decrease in the observation group was greater(P<0.05). The CMT of the observation group was smaller than that of the control group 12 weeks after treatment, and the difference was statistically significant(P<0.05). After treatment, BCVA was improved in both groups, and the improvement in the observation group was better than that in the control group(P<0.05). The level of BCVA in the observation group was better than that in the control group 12 weeks after treatment, and the difference was statistically significant(P<0.05). There were no complications such as intraocular hypertension, anterior chamber inflammatory exudation, visual field scotoma, subretinal fibrosis, and choroidal neovascularization(CNV) in both groups after treatment. Conclusion　The effect of two-time treatment with micropulse laser is better than that of single treatment with micropulse laser on DME.]]> 2022/11/2 11:21:28 LIU Lu-hong, LI Min, ZENG Si-ming, et al. 429true ［Abstract］　Objective　To investigate the application effect of transverse and longitudinal combined positioning method on minimally invasive resection of breast lumps. Methods　Six hundred and forty-eight breast lump patients who underwent vacuum-assisted minimally invasive breast atherectomy in the Second People′s Hospital of Liangshan Yi Autonomous Prefecture from March 2021 to April 2022 were selected. Among them, there were 24 cases of complex breast cysts, 489 cases of single breast fibroma, and 135 cases of multiple breast fibroma. According to different surgical positioning methods, the patients were divided into the control group(receiving treatment by using transverse positioning method, 306 cases) and the observation group(receiving treatment by using transverse and longitudinal combined positioning method, 342 cases). The operation time, intraoperative blood loss, postoperative complications and residual tumors were compared between the two groups. Results　The operation time of the observation group was shorter than that of the control group［(6.14±1.86)min vs (8.90±2.86)min］, and the intraoperative blood loss of the observation group was less than that of the control group［(4.29±1.38)ml vs (6.94±2.15)ml］, and the differences were statistically significant(P<0.05). In the control group, postoperative hemorrhage occurred in 2 cases, hematoma in 12 cases, and subcutaneous ecchymosis in 13 cases. In the observation group, postoperative hemorrhage occurred in 1 case, hematoma in 8 cases, and subcutaneous ecchymosis in 9 cases. The incidence of postoperative complications in the observation group was significantly lower than that in the control group(3.22% vs 8.82%; P<0.05). The breast color ultrasound was reexamined 3 months after operation, and the results showed that there were 11 cases(3.59%) with residual tumors in the control group and 4 cases(1.17%) with residual tumors in the observation group. There was a statistically significant difference in the residual tumor rate between the two groups after operation(P<0.05). Conclusion　In the minimally invasive resection of breast lumps, the transverse and longitudinal combined positioning method allows the operator to determine the spatial position of the atherectomy probe and the lumps more accurately, which is helpful for accurate excision of the lumps, with short operation time, less bleeding, low rates of complications and residual tumors. The method is worthy of clinical promotion.]]> 2022/11/2 11:21:28 LI Zhi-ling, LIU Cheng-kuan, YANG Hai-tao, et al. 428true ［Abstract］　Objective　To explore the predictive value of inflammatory indicators on the prognosis of patients with esophageal squamous cell carcinoma undergoing radical concurrent chemoradiotherapy. Methods　The clinical data of 154 patients with esophageal cancer who underwent radical concurrent chemoradiotherapy in the Department of Oncology of Suqian Hospital Affiliated to Xuzhou Medical University from January 2016 to December 2017 were retrospectively analyzed. The receiver operating characteristic(ROC) curve was used to analyze the predictive efficacy of systemic immune inflammation index(SII), prognostic nutritional index(PNI), neutrophil to lymphocyte ratio(NLR), platelet to lymphocyte ratio(PLR) and lymphocyte to monocyte ratio(LMR) on the overall survival of the patients before treatment. Survival curves were drawn by using Kaplan-Meier method to compare the survival of different groups. Cox regression was used to analyze the factors affecting the survival and prognosis of the patients, and the screened indicators were used to establish a nomogram prediction model through R language software. Results　The results of ROC curve analysis showed that the levels of SII, NLR, PLR, LMR and PNI before treatment could effectively predict the survival and prognosis of the patients(P<0.05). The results of Kaplan-Meier survival curve analysis showed that the survival prognosis of the SII≤794.8 group was better than that of the SII>794.8 group(P<0.05); the survival prognosis of the NLR≤3.25 group was better than that of the NLR>3.25 group(P<0.05), and the survival prognosis of the PLR≤125.65 group was better than that of the PLR>125.65 group, and the differences were statistically significant(P<0.05). The results of multivariate Cox regression analysis showed that Eastern Cooperative Oncology Group(ECOG) score(HR=1.774), T stage(HR=1.749), lymph node metastasis(HR=2.147), and SII(HR=2.513) were independent factors affecting the survival and prognosis of the patients(P<0.05). The C-index of the nomogram prediction model established based on these indicators was 0.796(95%CI: 0.757-0.871), which had good accuracy. Conclusion　SII is an independent factor affecting the survival and prognosis of the patients with esophageal squamous cell carcinoma undergoing radical chemoradiotherapy. The prediction model established based on SII and other indicators has good accuracy and can be used for clinical reference.]]> 2022/11/2 11:21:28 LIU Zi-hao, LU Xi-yan, JIN Xiao-wei, et al. 427true ［Abstract］　Objective　To analyze the effect of apolipoprotein E(APOE) ε4 genotype on cerebral blood flow in patients with Alzheimer′s disease(AD). Methods　Thirty-one AD patients admitted to Shenzhen Luohu District People′s Hospital from April 2020 to April 2021 were selected as the observation group, and other 25 elderly individuals with normal cognitive function during the same period were selected as the control group. Cognitive function was assessed by the Mini-mental State Examination(MMSE) scale in all the patients. The mean blood flow velocity(MFV) and pulsatility index(PI) of intracranial arteries were detected by transcranial Doppler sonography(TCD). The APOE genotype of the AD patients was detected by quantitative real-time polymerase chain reaction(qRT-PCR), and the effect of APOEε4 genotype on cerebral blood flow in the AD patients was analyzed. Results　The MFV values of the bilateral middle cerebral artery(MCA), anterior cerebral artery(ACA), posterior cerebral artery(PCA), vertebral artery(VA) and basilar artery(BA) in the observation group were lower than those in the control group, and the PI of the observation group was higher than that of the control group, and the differences were statistically significant(P<0.05). The results of Pearson correlation analysis showed that the MMSE score of the AD patients was positively correlated with MFV of intracranial arteries(P<0.05), and negatively correlated with PI(P<0.05). The results of receiver operator characteristic(ROC) curve analysis showed that both MFV and PI of intracranial arteris had application value in the diagnosis of AD(P<0.05), and the AUC of each index was more than 0.70. Among the 31 AD patients, 26 cases underwent APOE gene detection, of whom 11 cases(42.31%) were APOEε4 genotype. The MMSE scores of the APOEε4 genotype group were lower than those of the non-APOEε4 genotype group, and the differences were statistically significant［(19.50±8.62)points vs (24.15±5.14)points; t=2.322, P=0.030］. The MFV of the intracranial arteries in the APOEε4 genotype group was lower than that in the non-APOEε4 genotype group, and the PI in the APOEε4 genotype group was higher than that in the non-APOEε4 genotype group, and the differences were statistically significant(P<0.05). Conclusion　AD patients have cerebral hemodynamic changes characterized by decreased MFV and increased PI. The degree of cognitive impairment is significantly correlated with cerebral hemodynamic changes, and the patients carrying the APOEε4 genotype have more severe cognitive impairment and cerebral hemodynamic changes.]]> 2022/11/2 11:21:28 GUO Qi-wen, CHEN Chun-chun, XU Chun-yan, et al. 426true ［Abstract］　Objective　To analyze the results of urine metabolism and bacterial culture in patients with infectious kidney stones. Methods　Eighty-seven patients with infectious kidney stones who were admitted to First Teaching Hospital of Tianjin University of Traditional Chinese Medicine from January 2015 to January 2018 were selected. According to different stone compositions, the patients were divided into the simple infectious kidney stone group(magnesium ammonium phosphate and/or carbonated apatite, n=32) and the mixed infectious kidney stone group(magnesium ammonium phosphate and/or carbonated apatite+calcium oxalate, n=55). The general clinical data, urine metabolism test results, urine bacterial culture results and recurrence were compared between the two groups. Results　There were no significant differences in gender, age, complicated diabetes mellitus and family history of urolithiasis between the two groups(P>0.05). The proportion of the patients with a history of urinary tract infection and chronic urinary tract infection factors in the simple infectious kidney stone group was greater than that in the mixed infectious kidney stone group, and the difference was statistically significant(P<0.05). The positive rate of Escherichia coli in the mixed infectious kidney stone group was higher than that in the simple infectious kidney stone group, and the positive rate of Proteus species in the mixed infectious kidney stone group was lower than that in the simple infectious kidney stone group, and the differences were statistically significant(P<0.05). The incidence rates of hypercalciuria, hyperoxaluria and hyperuricuria in the mixed infectious kidney stone group were higher than those in the simple infectious kidney stone group, and the differences were statistically significant(P<0.05). The results of reexamination after operation showed that the proportion of patients with recurrent infection and new stones in the simple infectious kidney stone group was higher than that in the mixed infectious kidney stone group, and the differences were statistically significant(71.88% vs 32.73%, 50.00% vs 20.00%; P<0.05). Conclusion　Compared with the patients with simple infectious kidney stones, the patients with mixed infectious kidney stones have a higher incidence of abnormal urine metabolism, which may be related to the cause of the stones. Therefore, in addition to controlling infection, these patients should also be treated for their abnormal urine metabolism.]]> 2022/11/2 11:21:28 QIAO Peng-fei, YI Hu, TIAN Zhen-tao, et al. 425true ［Abstract］　Objective　To investigate the changes of the levels of blood inflammatory markers in patients with cervical cancer and their clinical significances. Methods　The clinical data of 94 patients with cervical cancer(the cervical cancer group) who were admitted to Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region from January 2017 to December 2020 were retrospectively analyzed, and 94 healthy individuals who came to the hospital for health check-up during the same period were selected as the control group. According to the clinical characteristics of the cervical cancer patients, they were further divided into different subgroups: squamous cell carcinoma group(74 cases) and adenocarcinoma group(20 cases); tumor diameter <4 cm group(70 cases) and tumor diameter ≥4 cm group(24 cases); postoperative pathological stageⅠA-ⅠB₂ group(63 cases) and postoperative pathological stageⅠB₃-ⅢC₂ group(31 cases). The levels of C-reactive protein(CRP), albumin(ALB), neutrophil(NEUT), lymphocyte(LY), platelet(PLT), white blood cell(WBC), C-reactive protein to albumin ratio(CAR), neutrophil to lymphocyte ratio(NLR), platelet to lymphocyte ratio(PLR) and prognostic nutritional index(PNI) were compared among the groups. Results　Compared with those in the control group, the levels of ALB and PNI in the cervical cancer group were low, and the levels of CAR, NLR and platelet to lymphocyte ratio(PLR) in the cervical cancer group were high, and the differences were statistically significant(P<0.05). The results of Pearson correlation analysis showed that PLR was positively correlated with NLR(r=0.654, P=0.000), and PNI was negatively correlated with PLR and NLR(r=-0.352, P=0.000; r=-0.250, P=0.000). The level of PLR in the adenocarcinoma group was significantly higher than that in the squamous cell carcinoma group(P<0.05). Compared with that in the tumor diameter <4 cm group, the level of PLT in the tumor diameter ≥4 cm group was high, and the difference was statistically significant(P<0.05). Compared with those in the postoperative pathological stage ⅠA-ⅠB₂ group, the levels of PLR and PLT in the postoperative pathological stageⅠB₃-ⅢC₂ group were high, and the differences were statistically significant(P<0.05). Conclusion　Elevated levels of CAR, NLR and PLR in cervical cancer patients may help in the differential diagnosis of cervical cancer. PLR has certain guiding significance for the pathological staging of cervical cancer.]]> 2022/11/2 11:21:28 LIU Zi-yu, XIONG Chun-qiu, HUANG Fei-feng, et al. 424true ［Abstract］　Objective　To construct a short hairpin ribonucleic acid(shRNA) lentiviral vector targeting to RelA(p65) gene and to verify its function. Methods　Three pairs of RNA interference sequences targeting to RelA gene were designed, and their corresponding shRNA sequences were synthesized. After annealing of shRNA to form double-stranded oligo sequence, the recombinant plasmid was constructed by gene recombination technique. The correct recombinant plasmid was used for lentivirus packaging and titer determination after polymerase chain reaction(PCR) and sequencing identification of the colony were performed. The lentivirus with the best interference effect on RelA gene in HepG2 cell line was screened by Western blot, and the effect of Lenti-shRelA on cell proliferation viability was detected by cell count kit-8(CCK-8). Results　The results of sequencing analysis showed that the recombinant lentivirus vector was consistent with the designed reference sequence, suggesting that the recombinant lentivirus vector was successfully constructed. The titers of the recombinant lentiviruses of Y4056, Y21318, Y21319 and Y21320 were 3.13×10⁸ TU/ml, 2.97×10⁸ TU/ml, 2.51×10⁸ TU/ml and 3.40×10⁸ TU/ml, respectively. After lentiviruses Lenti-shRelA(Y21318, Y21319, Y21320, Y4056) were infected with HepG2 cells, the experimental results of Western blot showed that Y21320 had the best interference effect on RelA gene in HepG2 cell line. The experimental results of CCK-8 showed that RelA knockdown significantly inhibited cell proliferation viability. Conclusion　In this study, the shRNA lentiviral vector targeting to RelA gene is successfully constructed, which can effectively down-regulate the expression of RelA in HepG2 cells and inhibit the cell proliferation, laying a foundation for further research on the mechanism of RelA in the occurrence and development of liver cancer.]]> 2023/10/12 10:25:14 HUO Yun-fei, GAO Ming-hui, DOU Shuang-shuang, et al. 423true ［Abstract］　Objective　To investigate the effect of sivelestat sodium on perioperative acute lung injury(ALI) in patients undergoing acute Stanford type A aortic dissection(AAAD) surgery. Methods　Seventy-two patients with AAAD who underwent emergency surgical treatment in the Operating Room of Henan Provincial Chest Hospital from December 2021 to June 2022 were recruited and divided into sivelestat sodium group(group S) and ulinastatin group(group U) by random number table method, with 36 cases in each group. The patients in the group S were intravenously infused with sivelestat sodium injection via infusion pumps at a rate of 0.2 mg/(kg·h) 10 minutes before anesthesia until the mechanical ventilation was weaned off. The patients in the group U were given ulinastatin intravenously at a dose of 20 000 U/kg 10 minutes before anesthesia until the mechanical ventilation was ended and weaned off. The radial artery blood was collected for blood gas analysis before skin incision and after induction of anesthesia(T₁), immediately after operation(T₂), 24 hours(T₃) and 48 hours(T₄) after operation, and the alveolar-arterial oxygen partial pressure difference(P_A-a DO₂), alveolar oxygenation index(OI) and respiratory index(RI) were calculated. The central venous blood was drawn from T₁ to T₄ time points to determine the levels of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and interleukin-8(IL-8). The peripheral venous blood was drawn 1 day before operation, and 1 day and 3 days after operation, and white blood cell count(WBC), neutrophil count(NEUT), neutrophil percentage(NEUT%) and the level of C-reactive protein(CRP) were determined. The patients′ incidence rates of postoperative pulmonary complications(PPCs) and adverse reactions were recorded. Results　Compared with those in the group U, the patients in the group S had shorter postoperative mechanical ventilation time and intensive care unit(ICU) stay time, and the difference was statistically significant(P<0.05). At the T₂ to T₄ time points, the levels of P_A-a DO₂ and RI in the group S were significantly lower than those in the group U(P<0.05), and the level of OI in the group S was significantly higher than that in the group U(P<0.05), and the serum levels of TNF-α, IL-6 and IL-8 in the group S were significantly lower than those in the group U(P<0.05). Compared with those 1 day before surgery, the levels of WBC, NEUT, NEUT% and CRP in the two groups were significantly increased 1 day and 3 days after operation(P<0.05), and the levels in the group S were lower than those in the group U(P<0.05). The incidence rates of hypercapnia, hypoxemia, newly emerging pulmonary rales and bronchospasm in the group S were lower than those in the group U after operation, and the differences were statistically significant(P<0.05). Conclusion　Sivelestat sodium can inhibit the inflammatory response in patients undergoing AAAD surgery, and reduce perioperative ALI, and lower the incidence of PPCs, and improve the early prognosis of the patients.]]> 2023/10/12 10:25:14 WU Guang-ling, LIU Si-qing, ZHOU Jun-hui, et al. 422true ［Abstract］　Objective　To study the effect of miR-138-5p on the resistance of breast cancer cells to tamoxifen(TAM) and its mechanism. Methods　Breast cancer cells MCF-7 and TAM resistant cell line MCF-7/TAM were cultured in vitro. Normal control group(MCF-7 cells), drug-resistant control group(MCF-7/TAM cells), LV-NC group(MCF-7/TAM cells+lentivirus empty vector) and LV-miR-138-5p mimics group(MCF-7/TAM cells+miR-138-5p mimics lentivirus) were set up. The expression level of miR-138-5p in each group was detected by quantitative real-time polymerase chain reaction(PCR)(qRT-PCR). The inhibition rate of cell proliferation in each group was detected by methyl thiazolyl tetrazolium(MTT) assay. The apoptosis rate in each group was detected by flow cytometry. The number of autophagosomes in each group was detected by monodansylcadaverine(MDC) staining. The protein expression levels of LC3Ⅱ, Beclin-1 and Bcl-2 in each group were detected by Western blot. Results　Compared with those in the normal control group, the expression levels of miR-138-5p, proliferation inhibition rate, apoptosis rate and Bcl-2 protein in the drug-resistant control group and the LV-NC group were significantly decreased(P<0.05), while the expression levels of protein LC3Ⅱ and Beclin-1 and the number of autophages were significantly increased(P<0.05). After overexpression of miR-138-5p, cell proliferation inhibition rate, apoptosis rate and protein Bcl-2 expression were significantly increased(P<0.05), while the expression levels of protein LC3Ⅱ and Beclin-1 and the number of autophagosomes were significantly decreased(P<0.05). Conclusion　Up-regulation of miR-138-5p may decrease the resistance of breast cancer cells to tamoxifen by inhibiting autophagy.]]> 2023/10/12 0:00:00 YAO Hao-ming, LI Ying 421true ［Abstract］　Objective　To compare the clinical application effects of different pretreatment balloons on treatment of coronary artery disease with drug-coated balloon(DCB). Methods　One hundred and fifty patients with angina pectoris of coronary artery disease who were admitted to Cangzhou People′s Hospital from October 2020 to October 2021 were recruited and divided into non-compliant balloon group, spinous balloon group and cut balloon group by random number table method, with 50 cases in each group, and non-compliant balloon, spinous balloon and cut balloon were pretreated in the three groups respectively before DCB was placed. Coronary angiography was performed before operation, immediately after operation, and 6 and 12 months after operation. The success rate of interventional therapy, operation time, one-time balloon passing rate, elastic retraction rate, minimum inner diameter of the lesion vessel, coronary stenosis rate, target vessel restenosis, thrombolysis in myocardial infarction(TIMI) grade 3 immediately after operation, vascular dissection, emergency target vessel reconstruction and major adverse cardiovascular events(MACE) were compared among the three groups. Results　The success rate of interventional therapy in the cut balloon group was higher than that in the non-compliant balloon group and the spinous balloon group immediately after operation, and 6 and 12 months after operation(P<0.017). The proportion of TIMI grade 3 immediately after operation in the cut balloon group was higher than that in the non-compliant balloon group and the spinous balloon group(P<0.017). The incidence rates of elastic retraction, vascular dissection and emergency target vessel reconstruction in the cut balloon group were significantly lower than those in the non-compliant balloon group(P<0.017, P<0.05), but the one-time balloon passing rate in the cut balloon group was lower than that in the non-compliant balloon group and the spinous balloon group(P<0.017). The operation time in the cut balloon group was longer than that in the non-compliant balloon group and the spinous balloon group(P<0.05). The minimum inner diameter of the lesion vessel in the three groups decreased gradually, and the coronary stenosis rate increased gradually. The minimum inner diameter of the lesion vessel in the cut balloon group was larger than that in the non-compliant balloon group and the spinous balloon group 6 and 12 months after operation, and the coronary stenosis rate in the cut balloon group was significantly lower than that in the non-compliant balloon group and the spinous balloon group(P<0.05). The incidence rates of target vessel restenosis and MACE in the cut balloon group were significantly lower than those in the non-compliant balloon group(P<0.017). Conclusion　Compared with that of non-compliant balloon and spinous balloon, the predilation of cut balloon can reduce the incidence of vascular dissection and elastic retraction rate, and improve the immediate effect of coronary intervention, and maintain the patency of the lesion lumen, and reduce the risk of target vessel restenosis and MACE.]]> 2023/10/12 10:25:14 ZHAO Dian-ru, GE Mei-qin, SHEN Qiao-li, et al. 420true ［Abstract］　Objective　To observe the application effect of dynamic accurate positioning software assisted soft channel drainage technology on hypertensive cerebral hemorrhage(HICH). Methods　The clinical data of 254 patients with HICH who were admitted to Daqing Oilfield General Hospital from January 2020 to January 2022 were selected. According to different localization methods of intracranial hematoma, the patients were divided into control group(124 cases, using the traditional surgical puncture method) and observation group(130 cases, using the puncture method assisted by drilling and positioning software). The clinical efficacy, extubation time, duration of hospitalization and the changes in the amount of hematoma, Chinese Stroke Scale of Clinical Neurologic Deficit Grade(CSS) scores, Babbitt index(BI) score, and Glasgow outcome scores(GOS) were compared between the two groups. The occurrence of complications was recorded in the two groups. Results　The total clinical effective rate of the observation group was significantly higher than that of the control group(93.08% vs 81.45%; P<0.05). The extubation time and the duration of hospitalization in the observation group was shorter than that in the control group, and the difference was statistically significant(P<0.05). The amount of hematoma in the observation group was significantly less than that in the control group 1 day and 2 days after operation(P<0.05). After treatment, the CSS scores in both groups were lower than those before treatment, and the BI scores were higher than those before treatment, and the differences were statistically significant(P<0.05), and the CSS scores of the observation group were lower than those of the control group, and the BI scores were higher than those of the control group, and the differences were statistically significant(P<0.05). The GOS of the observation group were higher than those of the control group, and the incidence of pulmonary infection in the observation group was lower than that in the control group, and the differences were statistically significant(P<0.05). Conclusion　Dynamic accurate positioning software assisted soft channel drainage technology is helpful for rapid drainage of hematoma, and promotes the patients′ postoperative neurological function recovery, and improves the clinical efficacy.]]> 2023/10/12 10:25:14 TANG Hai-tao, LIU Dong, XU Yang, et al. 419true ［Abstract］　Objective　To observe the clinical efficacy of external application of Yuying Powder combined with Diclofenac Sodium Sustained-release Tablets in treatment of pain caused by subacute thyroiditis(SAT). Methods　One hundred patients with pain caused by SAT who were admitted to the Department of Endocrinology of Hebei Hospital of Traditional Chinese Medicine from December 2020 to July 2021 were recruited and divided into observation group and control group by random number table method, with 50 cases in each group. The control group was treated with oral Diclofenac Sodium Sustained-release Tablets, while the observation group was treated with external application of Yuying Powder on the basis of the treatment regimen of the control group. Both groups were treated for 2 weeks continuously. The time of thyroid pain disappearance, fever clearance time, thyroid function index, pain mediators index, clinical efficacy and recurrence were compared between the two groups. Results　The total clinical effective rate of the observation group was higher than that of the control group, and the difference was statistically significant(98.00% vs 84.00%; χ²=4.396, P=0.036). The time of thyroid pain disappearance and the fever clearance time in the observation group was shorter than that in the control group, and the difference was statistically significant(P<0.05). After treatment, the levels of erythrocyte sedimentation rate(ESR), free triiodothyronine(FT₃), free thyroxine(FT₄), prostaglandin E₂(PGE₂), 5-hydroxytryptamine(5-HT), bradykinin(BK) and C-reactive protein(CRP) in the observation group were lower than those in the control group, and the level of thyroid-stimulating hormone(TSH) in the observation group was higher than that in the control group, and the differences were statistically significant(P<0.05). There were no significant differences in the incidence of adverse reactions and the recurrence rate between the two groups(P>0.05). Conclusion　The combination of external application of Yuying Powder and oral Diclofenac Sodium Sustained-release Tablets has a good therapeutic effect on SAT-induced pain. It can safely and quickly relieve the patients′ pain symptoms and promote the recovery of thyroid function.]]> 2023/10/12 10:25:14 YU Xiang-hui, REN Xiao-ya, YANG Yu-fei, et al. 418true ［Abstract］　Objective　To observe the effects of infiltration between the popliteal artery and capsule of the posterior knee block through distal approach on perioperative hemodynamics and recovery quality in elderly patients undergoing total knee arthroplasty(TKA). Methods　Eighty-four elderly patients with TKA who were admitted to the Affiliated Hospital of Yan′an University from October 2019 to October 2021 were recruited and divided into observation group and control group by random number table method, with 42 cases in each group. The control group underwent infiltration between the popliteal artery and capsule of the posterior knee block through proximal approach, while the observation group underwent infiltration between the popliteal artery and capsule of the posterior knee block through distal approach. The hemodynamic indexes during the perioperative period［after completion of block(T₁), 10 minutes after operation(T₂), at the time of joint replacement(T₃) and at the end of the operation(T₄)］, quality of recovery, postoperative analgesic effect, range of motion of joints and incidence of adverse reactions were compared between the two groups. Results　At the T₁-T₄ time points, the mean arterial pressure(MAP) and heart rate(HR) in the observation group were lower than those in the control group, and the differences were statistically significant(P<0.05). The time of spontaneous breathing recovery, eye opening time and full waking time in the observation group was shorter than that in the control group, and the difference was statistically significant(P<0.05). The Visual Analogue Scale(VAS) scores at exercise and at resting state in the observation group were lower than those in the control group 12, 24 and 48 hours after operation, and the differences were statistically significant(P<0.05). The ranges of motion of joints in the observation group were greater than those in the control group 12, 24 and 48 hours after operation, and the differences were statistically significant(P<0.05). There was no significant difference in the incidence of postoperative adverse reactions between the two groups(9.52% vs 11.90%; χ²=0.000, P=1.000). Conclusion　Infiltration between the popliteal artery and capsule of the posterior knee block through distal approach can effectively regulate perioperative hemodynamics and improve recovery quality in elderly patients undergoing TKA, and their postoperative analgesic effects are good, which is conducive to rapid recovery of the patients after surgery.]]> 2023/10/12 10:25:14 SU Hong-li, LI Ling-xia, LIU Yan-ling, et al. 417true ［Abstract］　Objective　To investigate the relationship between serum alpha-enolase antibody(ENO1Ab) level and mild cognitive impairment(MCI) in Hashimoto′s thyroiditis(HT) patients with normal thyroid function. Methods　Ninety-eight HT patients with normal thyroid function who were admitted to the Affiliated Hospital of Xuzhou Medical University from February 2022 to February 2023 were recruited and divided into a normal cognitive function group(55 cases, 27 points≤Mini-Mental State Examination(MMSE) scores≤30 points) and an MCI group(43 cases, 21 points≤MMSE scores<27 points) according to the MMSE score. The thyroid function indexes and serum ENO1Ab levels were compared between the two groups. The influencing factors of MCI in the HT patients were analyzed by multivariate logistic regression. Receiver operating characteristic(ROC) curve analysis was used to evaluate the diagnostic efficacy of ENO1Ab for MCI in the HT patients. Results　The age, thyroid peroxidase antibody(TPOAb) and ENO1Ab levels in the MCI group were higher than those in the normal cognitive function group, and the education level in the MCI group was lower than that in the normal cognitive function group, and the differences were statistically significant(P<0.05). The results of correlation analysis showed that MMSE score was negatively correlated with age, TPOAb and ENO1Ab(P<0.05). The results of multivariate logistic regression analysis showed that older age and higher levels of TPOAb and ENO1Ab were independent risk factors for promoting MCI in the HT patients(P<0.05). The results of ROC curve analysis showed that ENO1Ab had application value in diagnosing HT complicated with MCI［AUC(95%CI)=0.745(0.648-0.843), P=0.001］, and its optimal cut-off value was 9.455 ng/ml. The corresponding sensitivity and specificity were 62.80% and 76.40%, respectively. Conclusion　The occurrence of MCI in the HT patients with normal thyroid function is positively correlated with ENO1Ab level, which should be paid attention to by clinicians.]]> 2023/10/12 10:25:14 WANG Yue, SUN Juan, HE Miao-miao, et al. 416true ［Abstract］　Objective　To analyze the pregnancy outcomes and influencing factors of fresh single cleavage and single blastocyst transfer cycle. Methods　The clinical data of patients who underwent a total of 5 543 cycles of cleavage stage and blastocyst stage single embryo transfer(SET) from October 2002 to October 2020 in the Reproductive Center of the Maternal and Child Health Care Hospital of Guangxi Zhuang Autonomous Region were retrospectively analyzed. According to different embryo transfer periods, they were divided into day 3 cleavage embryo group(D3 group, 1 229 cases) and day 5 blastocyst group(D5 group, 4 314 cases). The clinical data and pregnancy outcomes were compared between the two groups, and the influencing factors of pregnancy outcomes in SET cycle were analyzed by binary logistic regression. Results　The clinical pregnancy rate in the SET cycle was 48.13%(2 668/5 543) , and the live birth rate was 39.49%(2 189/5 543) and the multiple birth rate was 2.89%(77/2 668). The results of binary logistic regression analysis showed that age and embryonic development period were the co-influencing factors of SET clinical pregnancy outcomes and live birth outcomes(P<0.05). The results of stratified analysis showed that there were no significant differences in the clinical pregnancy rate(54.55% vs 54.76%) and the live birth rate(49.09% vs 47.21%) between the D3 group and the D5 group, both with age ≤30 years and the number of good quality embryos >3(P>0.05). Conclusion　The selection of single cleavage stage and single blastocyst stage transfer in fresh cycle can obtain better clinical pregnancy rate and live birth rate. The clinical pregnancy rate and live birth rate of single cleavage stage transfer can be similar to those of single blastocyst stage transfer in a suitable population.]]> 2023/10/12 10:25:14 HUANG Ying-qin, HUANG Dong-mei, HUANG Ling, et al. 415true ［Abstract］　Objective　To explore the mechanism of circular ribonucleic acid(cricRNA) circ-FOXM1 reducing the inhibitory effect of crizotinib on lung cancer cells. Methods　A crizotinib-resistant lung cancer cell line H2228/CR was constructed. Quantitative real-time polymerase chain reaction(qRT-PCR) was used to detect the expressions of circ-FOXM1 in BEAS-2B, H2228 and H2228/CR cells. H2228 cells were divided into circ-FOXM1 overexpression group and overexpression control group. H2228/CR cells were divided into circ-FOXM1 silencing group and silencing control group. The half maximal inhibitory concentration(IC₅₀) of the cells was detected by using methyl thiazolyl tetrazolium(MTT) assay method. After the intervention of crizotinib, the apoptosis rate of the cells in each group was detected by flow cytometry, and the number of cell migration in each group was detected by Transwell assay. The expression levels of phosphorylated phosphatidylinositol 3 kinase(p-PI3K), phosphatidylinositol 3 kinase(PI3K), phosphorylated protein kinase B(p-AKT) and protein kinase B(AKT) in the cells of each group were detected by using Western blot assay. Results　The expression level of circ-FOXM1 in the H2228/CR cells was higher than that in the H2228 cells, and the expression level of circ-FOXM1 in the H2228 cells was higher than that in the BEAS-2B cells, and the differences were statistically significant(P<0.05). The IC₅₀ of crizotinib in the cells of the overexpression group was significantly higher than that in the cells of the overexpression control group(P<0.05). The IC₅₀ of crizotinib in the cells of the silencing group was significantly lower than that in the cells of the silencing control group(P<0.05). After the intervention of crizotinib, the apoptosis rate in the overexpression group was lower than that in the overexpression control group, and the number of cell migration, p-PI3K/PI3K ratio and p-AKT/AKT ratio in the overexpression group were higher than those in the overexpression control group, and the differences were statistically significant(P<0.05). The apoptosis rate in the silencing group was higher than that in the silencing control group, and the number of cell migration, p-PI3K/PI3K ratio and p-AKT/AKT ratio in the silencing group were lower than those in the silencing control group, and the differences were statistically significant(P<0.05). Conclusion　Circ-FOXM1 may reduce the inhibitory effect of crizotinib on lung cancer cells by activating PI3K/AKT signaling pathway.]]> 2023/10/12 10:25:14 XIA Yan-zhen, HUO Yin-ping, WANG Long-zhen, et al. 414true ［Abstract］　Objective　To analyse the effect of minimally invasive hematoma puncture combined with ventricle and lumbar cistern drainage in treatment of hypertensive cerebellar hemorrhage rupture into ventricles. Methods　Thirty patients with hypertensive cerebellar hemorrhage rupture into ventricles were selected from Hezhou Hospital of Traditional Chinese Medicine. All the patients received preoperative computed tomography(CT)-guided hematoma puncture combined with ventricle and lumbar cistern drainage, and the hematoma clearance and recovery were observed. Results　Among the 30 cases, 22 cases had better recovery, and 1 case died and 7 cases were disabled, including 5 cases of mild disability and 2 cases of severe disability. Conclusion　Minimally invasive hematoma puncture combined with ventricle and lumbar cistern drainage can rapidly reduce intracranial pressure, accelerate the clearance of hematoma, reconstruct the circulation of cerebrospinal fluid and improve the prognosis in treatment of hypertensive cerebellar hemorrhage rupture into ventricles.]]> 2023/10/12 10:25:14 ZHONG Xiang-qiu, LIU Qian 413true ［Abstract］　Objective　To analyze the clinical characteristics of ineffective esophageal motility(IEM). Methods　Three hundred and thirty-eight patients who underwent high-resolution esophageal manometry and 24-hour esophageal pH monitoring in West China Hospital of Sichuan University from July 2021 to July 2022 due to gastroesophageal reflux disease(GERD)-like symptoms or dysphagia were selected. According to the occurrence of IEM, the patients were divided into normal esophageal motility group(n=264) and IEM group(n=74). The demographic characteristics, data of esophageal manometry, percentage of acid exposure time(AET), clinical symptoms and response to proton pump inhibitor therapy were compared between the two groups. Results　The proportion of males in the IEM group was significantly higher than that in the normal esophageal motility group, and the mean basal esophagogastric junction(EGJ) pressure in the IEM group was significantly lower than that in the normal esophageal motility group(P<0.05). There were no significant differences in age, body mass index and EGJ type between the two groups(P>0.05). There were no significant differences in the incidence rates of acid reflux/heartburn, chest pain, throat discomfort, dysphagia and non-specific symptoms between the two groups(P>0.05). The proportions of total AET>4.0%, upright AET>6.0% and supine AET>2.0% in the IEM group were significantly higher than those in the normal esophageal motility group(P<0.05). There was no statistically significant difference in the effective rate of acid-suppression therapy between the two groups(52.08% vs 47.37%; χ²=0.341, P=0.559). The results of multivariate logistic regression analysis showed that IEM and EGJ type Ⅱ/Ⅲ were independent risk factors for promoting GERD(P<0.05), and higher mean basal EGJ pressure was a protective factor against GERD(P<0.05). Conclusion　IEM is associated with increased exposure to esophageal acid, and the clinical manifestations are not specific, and IEM has no significant effect on the efficacy of proton pump inhibitors.]]> 2023/8/31 17:39:32 LU Yao-yao, ZENG Yu, YANG Jin-lin, et al. 412true ［Abstract］　Objective　To analyze the effect of levels of triglyceride-rich lipoproteins(TGRLs) on clinical outcomes in elderly patients with non-diabetic ST-segment elevation myocardial infarction(STEMI) after percutaneous coronary intervention(PCI). Methods　The clinical data of 311 non-diabetic STEMI patients admitted to Qinghai Province Cardiovascular and Cerebrovascular Disease Specialist Hospital from February 2018 to October 2020 were retrospectively analyzed, all of whom received coronary angiography(CAG) and PCI. The patients′ blood samples were collected before PCI for detection of levels of TGRLs, and the patients were followed up after PCI and their incidence of major adverse cardiovascular events(MACE) and all-cause mortality were observed. Results　The results of multivariate Cox regression analysis showed that higher Global Registry of Acute Coronary Event(GRACE) scores and levels of TGRLs at admission were independent risk factors for MACE(P<0.05); higher levels of TGRLs were an independent risk factor for all-cause mortality(P<0.05). The results of receiver operating characteristic(ROC) curve analysis showed that levels of TGRLs could effectively predict the occurrence of MACE［AUC(95%CI)=0.825(0.749-0.902)］ and all-cause mortality［AUC(95%CI)=0.910(0.858-0.962)］ after surgery(P<0.001), and the best cut-off value for predicting MACE was 0.835 mmol/L, and the corresponding sensitivity and specificity were 73.3% and 79.7%, respectively; the best cut-off value for predicting all-cause mortality was 0.885 mmol/L, and the corresponding sensitivity and specificity were 83.3% and 86.7%, respectively. Conclusion　High level of TGRLs is a risk factor for MACE and all-cause mortality after PCI in elderly non-diabetic STEMI patients, which is helpful to guide the clinical grading management of the patients.]]> 2023/8/31 17:39:32 HAN Xiao-juan, YAN Zhan-guang, HOU Hai-wen 411true ［Abstract］　Objective　To investigate the effect of preoperative aerosol inhalation of penehyclidine hydrochloride on alleviating non-ventilated lung injury during one-lung ventilation(OLV) and its mechanisms of action. Methods　Sixty patients who underwent the elective operation of thoracoscopic radical resection for lung cancer in Luoyang Central Hospital Affiliated to Zhengzhou University from March 2021 to May 2022 were recruited and divided into observation group and control group by random number table method, with 30 cases in each group. Immediately after induction of anesthesia, the patients in the control group inhaled ipratropium bromide by aerosol inhalation, and the patients in the observation group inhaled penehyclidine hydrochloride by aerosol inhalation. Partial pressure of arterial oxygen(PaO₂) and partial pressure of arterial carbon dioxide(PaCO₂) were measured immediately after induction of general anesthesia(T₀), and 0.5 hours(T₁), 1 hour(T₂) and 2 hours(T₃) after OLV. During the operation, the normal lung tissue specimens around the tumor were excised for pathological examination and lung injury scores were calculated. Apoptosis index(AI) was calculated by terminal-deoxynucleotide transferase mediated nick end labeling(TUNEL) method. The expression levels of apoptosis and autophagy marker protein in the lung tissues were detected by Western blot. Results　The morphology and structure of the lung tissues in the control group were obviously damaged under light microscope, while the damage of the lung tissues in the observation group was significantly reduced. During the observation period, PaO₂ showed a decreasing trend and PaCO₂ showed an increasing trend in both groups, and the change range of the observation group was smaller(P<0.05), and the differences between the two groups were statistically significant at the time points of T₁ -T₃(P<0.05). Compared with those in the control group, the lung injury scores, AI, B-lymphocytoma-2 related X protein(Bax), microtubule-associated protein 1 light chain 3(LC3)-Ⅱ, the level of autophagy marker protein Beclin-1 and the ratio of LC3-Ⅱ/LC3-Ⅰin the observation group were significantly decreased(P<0.05), while the levels of B-lymphocytoma-2(Bcl-2) and LC3-Ⅰ protein and the ratio of Bcl-2/Bax were significantly increased(P<0.05). Conclusion　Preoperative aerosol inhalation of penehyclidine hydrochloride can alleviate the non-ventilated lung injury during OLV, and the inhibitions of lung tissue apoptosis and autophagy play important roles.]]> 2023/8/31 17:39:32 KANG Xu-ming, WU Shuang-ting, NI Kun-qiang, et al. 410true ［Abstract］　Objective　To study the expressions of foxhead box protein p3(Foxp3) and interleukin-15(IL-15) in perianal abscess tissues and their correlations with disease recurrence. Methods　Ninety-eight patients with perianal abscess who underwent surgical treatment in Shunyi Hospital of Beijing Hospital of Traditional Chinese Medicine from January 2019 to July 2020 were selected. The intraoperative abscess wall tissues and normal tissues around the abscess wall of the patients were collected. The expressions of Foxp3 and IL-15 were detected by immunohistochemical staining. The correlations of Foxp3 and IL-15 expressions in the perianal abscess tissues with the patients′ clinicopathological features and disease recurrence were analyzed. Results　Foxp3 was mainly expressed in the cell nucleus region, and IL-15 protein was mainly expressed in the cytoplasm and cell membrane. The positive expression rates of Foxp3 and IL-15 protein in the abscess wall tissues were higher than those in the normal tissues, and the differences were statistically significant(P<0.05). The expressions of Foxp3 and IL-15 in the abscess wall tissues were significantly correlated with abscess diameter, healing time and preoperative anal fistula formation(P<0.05), but were not significantly correlated with gender, age, abscess location and course of disease(P>0.05). The results of Cox regression analysis showed that preoperative anal fistula, Foxp3 positive and IL-15 positive were independent risk factors for disease recurrence in patients with perianal abscess(P<0.05). Conclusion　The expressions of Foxp3 and IL-15 are increased in perianal abscess tissues, which is closely related to the patients′ clinicopathological features and disease recurrence.]]> 2023/8/31 17:39:32 ZHANG Li, TIAN Ze-yang, SUN Lin-feng 409true ［Abstract］　Objective　To analyze the correlation between serum alpha 1-antitrypsin(A1AT) level and type 2 diabetes mellitus(T2DM) complicated with non-alcoholic fatty liver disease(NAFLD) and liver fibrosis. Methods　One hundred and seventeen T2DM patients complicated with NAFLD who were admitted to the Second People′s Hospital of Tibet Autonomous Region from March 2019 to March 2020 were enrolled as the T2DM+NAFLD group, and 80 T2DM patients(the T2DM group) and 80 healthy subjects(the control group) matched with the NAFLD+T2DM group in gender and age were enrolled during the same period. The serum levels of A1AT were detected by immunoturbidimetry, and liver fibrosis was evaluated by non-alcoholic fatty liver disease fibrosis score(NAFLDFS). Multivariate logistic regression was used to analyze the influencing factors of T2DM complicated with NAFLD. Receiver operating characteristic(ROC) curve was used to evaluate the diagnostic value of serum A1AT level in T2DM complicated with NAFLD. Spearman rank correlation was used to analyze the correlation between serum A1AT level and NAFLDFS. Results　The results of multivariate logistic regression analysis showed that higher levels of fasting blood glucose(FPG), fasting insulin(FINS), homeostasis model assessment(HOMA)insulin resistant index(HOMA-IR), triglyceride(TG), liver fat content(LFC) and A1AT were independent risk factors for NAFLD in the T2DM patients(P<0.05). The results of ROC curve analysis showed that serum A1AT level had the application value in diagnosing NAFLD in the T2DM patients［AUC(95%CI)=0.793(0.758-0.829), P<0.001］, and its optimal cut-off value was 2.91 g/L, and the corresponding sensitivity and specificity were 0.81 and 0.69, respectively. The results of Spearman rank correlation analysis showed that serum A1AT level in the T2DM complicated with NAFLD patients was positively correlated with NAFLDFS(r_s=0.635, P<0.001). Conclusion　Serum A1AT is up-expressed in the T2DM complicated with NAFLD patients, which can be used as a biochemical marker to assist the diagnosis of NAFLD and to evaluate the degree of liver fibrosis in T2DM patients.]]> 2023/8/31 17:39:32 ZE Na, Mimaciren, Zhaxizhuoma 408true ［Abstract］　Objective　To analyze the clinical characteristics of Clostridium difficile infection(CDI) in children and the risk factors of the disease recurrence. Methods　The clinical data of 138 pediatric patients with CDI who were hospitalized in the Department of Digestive Infection of Shanghai Children′s Hospital from September 2014 to October 2022 were selected. According to the disease recurrence after treatment, the pediatric patients were divided into recurrent group(n=39) and non-recurrent group(n=99). The clinical data were compared between the two groups, and the risk factors of CDI recurrence were analyzed by binary logistic regression. Results　Among the 138 pediatric patients with new-onset CDI, there were 81 males and 57 females, with a median age of 4.80 years. The proportions of the pediatric patients with diarrhea, pseudomembranous colitis and hospital-acquired CDI(HA-CDI) in the recurrent group were higher than those in the non-recurrent group, and the differences were statistically significant(P<0.05). The results of binary logistic regression analysis showed that pseudomembranous colitis and HA-CDI were independent risk factors for disease recurrence in children with CDI(P<0.05). Conclusion　Pseudomembranous colitis and HA-CDI are independent risk factors for disease recurrence in hospitalized children with CDI, which should be paid attention to by clinicians.]]> 2023/8/31 17:39:32 CAO Rong, LI Xiao-lu, WANG Yi-zhong, et al. 407true ［Abstract］　Objective　To explore the mechanism of edaravone in promoting cerebral angiogenesis and nerve function repair in ischemic stroke rats. Methods　Sixty specific pathogen free(SPF) male Sprague-Dawley(SD) rats were selected. Among the 60 SD rats, twelve rats were randomly selected as sham operation group, and the other 48 rats were made into an ischemic stroke model by blocking the blood flow in the middle cerebral artery, and the model rats were divided into ischemic stroke group, edaravone group, inhibitor group and edaravone+inhibitor group, with 12 rats in each group. Cognitive function was detected by Morris water maze, and cerebral infarction size was measured by 2, 3, 5-triphenyltetrazolium chloride(TTC) staining. The whole brain tissues were taken, frozen and sectioned, and the fluorescence intensities of Caveolin-1 /CD34 and doublecortin(DCX)/5-bromo-2′-deoxyuridine(BrdU) protein in the ischemic core area were observed by immunofluorescence method. The ischemic basal ganglia area of the brain tissues was taken. The expression levels of Caveolin-1, vascular endothelial growth factor(VEGF), brain-derived neurotrophic factor(BDNF) and tyrosine kinase receptor B(TrkB) were detected by Western blot. Results　Compared with those in the sham operation group, the rats in the ischemic stroke group had increased neurological function scores, increased escape latency and decreased number of crossing platforms, increased fluorescence intensities of Caveolin-1 and CD34, increased expressions of Caveolin-1 and VEGF proteins, and decreased expressions of BDNF, TrkB, DCX and BrdU proteins, and the differences were statistically significant(P<0.05). Compared with the ischemic stroke group, the edaravone group had reduced neural function scores and escape latency, and increased number of crossing platforms, and decreased cerebral infarction size, and the increased co-expressions of Caveolin-1 and CD34, and further increased expression levels of Caveolin-1 and VEGF, and increased expressions of BDNF, TrkB, DCX and BrdU proteins, and the differences were statistically significant(P<0.05). Compared with those in the edaravone group, the rats in the inhibitor group and the edaravone+inhibitor group had decreased expressions of Caveolin-1 and VEGF proteins, and decreased co-expressions of Caveolin-1 and CD34, and increased cerebral infarction size and escape latency, and the differences were statistically significant(P<0.05). Conclusion　The anti-ischemic stroke effect of edaravone is related to the activation of Caveolin-1/VEGF signaling pathway, and edaravone can improve cognitive function by promoting neuroangiogenesis, restoring blood supply in ischemic area and alleviating nerve function injury in rats.]]> 2023/8/31 17:39:32 WANG Wen-sheng·WANG Yue, SUN Ting 406true ［Abstract］　Objective　To analyse the epidemiological characteristics of acute respiratory tract infection(ARTI) caused by respiratory syncytial virus(RSV) in children in Hainan Province, and to provide reference for the prevention, diagnosis and treatment of RSV infection in children. Methods　The clinical data of 59 312 children with ARTI who were hospitalized in hospitals in Hainan Province from March 2012 to December 2021 were collected. The epidemiological and clinical data of the RSV infection cases were retrospectively analyzed. The immunoglobulin M(IgM) antibodies of nine respiratory pathogens in the patients′ serum including RSV, Mycoplasma pneumoniae, Legionella pneumophila, Chlamydia pneumoniae, Adenovirus, Coxiella burnetii, Parainfluenza virus, Parainfluenza A virus and Parainfluenza B virus were detected by indirect immunofluorescence method. Results　The positive rate of RSV-IgM in the 59 312 children with ARTI was 2.07%(1 226/59 312). From 2016 to 2018, the positive rate of RSV-IgM showed an increasing trend, and reached a high value in 2017 and 2018, which was 4.46% and 5.20%, respectively, and then showed a decreasing trend. The positive rate of RSV-IgM in the male children with ARTI was significantly higher than that in the female children with ARTI(2.21% vs 1.82%; χ²=10.714, P<0.001). The positive rate of RSV-IgM was the highest in summer(2.59%) and the lowest in autumn(1.70%). Among the 1 226 cases of RSV-IgM positive children, 485 cases(39.56%) were simple RSV-IgM positive; 741 cases(60.44%) had mixed infection. Among the 741 cases with mixed infection, 505 cases(41.19%) were infected with one other pathogen, and 191 cases(15.58%) were infected with two other pathogens, and 45 cases(3.67%) were infected with three other pathogens. For the RSV-IgM positive children, the proportion of clinically diagnosed pneumonia was the highest, and cough was the most common clinical symptom. The incidence of fever in children with mixed infection was higher than that in children with single RSV infection, and the difference was statistically significant(P<0.05). Conclusion　The total positive rate of RSV-IgM is 2.07% in hospitalized ARTI children in Hainan Province from 2012 to 2021. The incidence of RSV infection is highest in summer and lowest in autumn. RSV infection occurs in all age groups, with the highest RSV infection rate in infants and the lowest RSV infection rate in school age children. The infection rate of RSV in men is higher than that in women. RSV infection is often accompanied by one pathogen infection, and most of these pathogens are Mycoplasma pneumoniae. The main clinical symptoms of RSV infection are cough, fever, stuffy nose, running nose, wheezing and shortness of breath.]]> 2023/8/31 17:39:32 CHEN Hai-mei, ZHANG Yu-qing, CAI Si-ming, et al. 405true ［Abstract］　Objective　To investigate the clinical characteristics and treatment methods of pyoderma gangrenosum(PG). Methods　From May 2013 to May 2021, 35 patients with pyoderma gangrenosum who were treated in the Outpatient Department and the Ward of the Department of Dermatology of the People′s Hospital of Guangxi Zhuang Autonomous Region were retrospectively analyzed for their general data, causes, rash distribution, rash morphology, misdiagnosis, complications and treatment methods. Results　The ratio of male to female patients was 2.9∶1, and the age ranged from 29 to 72 years, with an average age of (41.5±8.5)years. Fifteen patients had definite predisposing factors, including trauma in 11 cases, insect bite in 3 cases and injection of drug abuse in 1 case. Among the 35 patients, there were 32 cases(91.43%) of ulcerative type, 2 cases(5.71%) of bullous type, 1 case(2.86%) of pustular type. At the initial stage of the disease, the most frequently misdiagnosed disease was bacterial soft tissue infection(32 cases, 91.43%). Skin lesions are usually 2 or more. There were 23 cases(65.71%) of skin lesions in the lower limbs, mainly in the calves. Eight patients(22.86%) were found to have concurrent or subsequent systemic diseases, including 5 patients(14.29%) with inflammatory bowel disease, 2 patients(5.71%) with multiple arthritis, and 1 patient(2.86%) with leukemia. The patients without complications or complicated with inflammatory bowel disease and polyarthritis had a better prognosis, while those with hematological diseases had a worse prognosis. The systemic treatment was based on the size of lesions and complications, and the dose of prednisone was 0.5-1 mg/(kg·d). After the infections were slightly controlled, 28 patients were subsequently given cyclosporine 2.5～5 mg/(kg·d). Conclusion　The clinical manifestations of PG are varied, and it is easy to be misdiagnosed at the initial stage of the disease, and a comprehensive judgment should be made according to clinical manifestations and treatment effects. Moderate to high dose corticosteroids can effectively control PG, and the combined corticosteroid therapy with cyclosporine is safe and effective.]]> 2023/8/31 17:39:32 XU Yu-xin, XIE Zhi, LI Yu-qiu, et al. 404true ［Abstract］　Objective　To investigate the effect of circular ribonucleic acid(CircRNA) septin 9(CircSEPT9) on malignant biological behavior of glioma cells by regulating micro ribonucleic acid-650(miR-650)/heat shock protein B1(HSPB1) axis. Methods　The expressions of CircSEPT9, miR-650 and HSPB1 protein in glioma tissues, tumor-adjacent tissues, human normal glial cells HEB and glioma cell lines LN18, SW1088 and U251 were detected by quantitative real time polymerase chain reaction(qRT-PCR) and Western blot, respectively. U251 cells were divided into control group(NC group), si-NC group, si-CircSEPT9 group, mimic NC group, miR-650 mimic group, si-CircSEPT9+inhibitor NC group, si-CircSEPT9+miR-650 inhibitor group, si-CircSEPT9+pcDNA group, and si-CircSEPT9+pcDNA-HSPB1 group. The expression levels of CircSEPT9 and miR-650 were detected by qRT-PCR. Western blot was applied to detect the expression levels of HSPB1, Cyclin D1, Caspase-3, matrix metalloproteinase(MMP)-2 and MMP-9 proteins. Double luciferase gene experiment was applied to verify the relationship between CircSEPT9 and miR-650, and the relationship between miR-650 and HSPB1. Results　In the glioma tissues and glioma cells, CircSEPT9 and HSPB1 were highly expressed, while miR-650 was low expressed. In the U251 cells, the expression levels of CircSEPT9 and HSPB1 were the highest, and the expression level of miR-650 was the lowest, and the differences were significant(P<0.05). Silencing CircSEPT9 or overexpressing miR-650 was able to inhibit U251 cell proliferation, invasion, migration and the expression levels of Cyclin D1, MMP-2 and MMP-9 proteins, and was able to promote cell apoptosis and Caspase-3 protein expression. The down-regulation of miR-650 or overexpression of HSPB1 could weaken the effect of CircSEPT9 silencing on the U251 cells. CircSEPT9 had a targeting relationship with miR-650, and miR-650 had a targeting relationship with HSPB1. Conclusion　Silencing CircSEPT9 may inhibit the expression of HSPB1 by up-regulating miR-650 through spongification, thereby inhibiting the proliferation, migration and invasion of U251 cells and promoting apoptosis.]]> 2023/8/31 17:39:32 REN Cheng-bo, ZHAO Li-xia, DU Xiao-meng, et al. 403true ［Abstract］　Objective　To investigate the application effect of Congo red staining for frozen sections of nerve specimens on the pathological diagnosis of peripheral neuropathy, and to explore the optimal conditions for experimental operation. Methods　One patient with amyloid peripheral neuropathy who was admitted to the Department of Neurology of Xuanwu Hospital of Capital Medical University in August 2022 was selected to take biopsy specimens of sural nerve. The frozen slices with thicknesses of 6 μm and 8 μm were prepared, and the dyeing time of Highman Congo red dye was set to 5 minutes, 10 minutes, 15 minutes and 20 minutes, respectively. The results of Congo red staining in the frozen sections were observed under ordinary microscope, birefringent polarized light microscope and immunofluorescence microscope, respectively. Results　When the dyeing time was 10 minutes, 15 minutes and 20 minutes, the deposition of specific substances could be observed in the slices of both thicknesses, and the color intensity increased with the increase of slice thickness. When the thickness of the section was 6 μm, the effect of staining for 5 minutes was unsatisfactory. However, when the thickness of the section was 8 μm, the effect of staining for 15 minutes was the best. The staining results observed under light microscope and fluorescence microscope were more conducive to the observer′s diagnosis. Conclusion　The frozen section of nerve specimens with a thickness of 8 μm and the staining time for 15 minutes are the appropriate conditions for Congo red staining, and the staining method has good application value in the pathological diagnosis of amyloid peripheral neuropathy.]]> 2023/8/4 12:14:58 DUO Jian-ying, CHEN hai, XU Min, et al. 402true ［Abstract］　Objective　To analyse the association of serum hypoxic inducible factor-1α(HIF-1α) level with symptomatic cerebral hemorrhage(sICH) and prognosis after intravenous thrombolysis in patients with acute ischemic stroke(AIS). Methods　Two hundred and twenty-nine AIS patients admitted to Xiyuan Hospital of China Academy of Chinese Medical Sciences from January 2019 to February 2021 were recruited, all of whom received intravenous thrombolytic treatment with recombinant tissue plasminogen activator(rt-PA) within 4.5 hours of onset. According to the occurrence of sICH 36 hours after treatment, the patients were divided into sICH group(25 cases) and non-sICH group(204 cases). The modified Rankin Scale(mRS) score was used to assess the patients′ functional outcomes 3 months after treatment. The serum HIF-1α level was determined by sandwich enzyme-linked immunosorbent assay. Results　The National Institute of Health Stroke Scale(NIHSS) scores, fasting blood glucose(FBG) and HIF-1α level in the sICH group were higher than those in the non- sICH group at admission, and the proportion of the patients with diabetes mellitus and previous history of stroke in the sICH group was higher than that in the non- sICH group, with statistically significant differences between the two groups(P<0.05). The results of receiver operating characteristic(ROC) curve analysis showed that serum HIF-1α level had application value in predicting the occurrence of sICH in the AIS patients［AUC(95%CI)=0.886(0.815-0.957), P<0.001］, and the optimal cut-off value was 1.96 ng/ml. The corresponding sensitivity and specificity were 88.0% and 78.4%, respectively. The results of Cox regression analysis showed that serum HIF-1α≥1.96 ng/ml before thrombolysis was a risk factor for sICH in the AIS patients(P<0.05). The results of Spearman rank correlation analysis showed that serum HIF-1α level was positively correlated with mRS scores after treatment for the patients(r_s=0.137, P=0.038). After adjusting the confounding factors such as age, gender, smoking history and history of complications, the results of multiple linear stepwise regression analysis showed that serum HIF-1α level was an independent factor affecting the mRS scores of the AIS patients after treatment(t=2.069, β=0.324, P=0.026). Conclusion　Elevated serum HIF-1α level is associated with an increased risk of secondary sICH and poor short-term prognosis after intravenous thrombolysis of rt-PA in AIS patients.]]> 2023/8/4 12:14:58 QI Jiang-xia, LI Hai-yan, LIU Yang, et al. 401true ［Abstract］　Objective　To analyze the relationship between metabolic parameters of positron emission tomography/computed tomography(PET/CT) and biological characteristics of lung adenocarcinoma and its predictive value for brain metastases. Methods　One hundred and three patients with lung adenocarcinoma who were admitted to Handan Central Hospital from February 2020 to April 2022 were recruited, and all the patients underwent PET/CT examination. The correlation between biological characteristics of lung adenocarcinoma and the maximum value of standard uptake value(SUV) and the maximum value of standard uptake value of lean body mass(SUL) of the patients′ lesions were analyzed. The predictive value of SUV maximum value and SUL maximum value for brain metastases of lung adenocarcinoma were explored through multivariate logistic regression and nomogram analysis. Results　Lymph node metastasis, T stage and differentiation degree were significantly correlated with SUV maximum value and SUL maximum value(P<0.05). The results of multivariate logistic regression analysis showed that T_3-4 stage, and higher levels of SUV maximum value and SUL maximum value were independent risk factors for brain metastases in patients with lung adenocarcinoma(P<0.05). The nomogram prediction model showed that SUV maximum value and SUL maximum value had higher predictive value for brain metastases in patients with lung adenocarcinoma, and the consistency index was 0.785 and 0.811, respectively. The results of decision curve analysis(DCA) showed that the net benefit rate of SUL maximum value in predicting brain metastases in patients with lung adenocarcinoma was better than that of SUV maximum value within the threshold range of 0.35 to 0.71. Conclusion　SUV maximum value and SUL maximum value in the lesions are correlated with the biological characteristics of lymph node metastasis, T stage and differentiation degree of lung adenocarcinoma. PET/CT examination can be used to predict the brain metastases of the patients as early as possible in clinic, so as to provide reference for clinical treatment and improve the prognosis of the patients.]]> 2023/8/4 12:14:58 YOU Zhi-xin, LIU Ai-min, FENG Xiao-wei, et al. 400true ［Abstract］　Objective　To analyze the correlation between serum miR-139-5p expression level and the short-term prognosis in patients with acute coronary syndrome(ACS). Methods　One hundred and seventy-six ACS patients who were admitted to Anhui No.2 Provincial People′s Hospital due to chest pain from January 2021 to September 2021 were recruited, including 100 patients with unstable angina pectoris(UAP)(UAP group) and 76 patients with acute myocardial infarction(AMI)(AMI group). Other 28 patients who were admitted to the hospital due to chest pain and were excluded from coronary heart disease by coronary angiography were selected as control group. On the next day of admission, the venous blood of the patients was drawn to detect the expression levels of serum miR-139-5p and vascular endothelial growth factor receptor-1(VEGFR-1), and the other clinical data of the patients were collected. All the patients were followed up for 6 months to record the occurrence of major adverse cardiovascular events(MACE). Results　Compared with the control group, the UAP group and the AMI group had higher serum expression levels of miR-139-5p and lower serum expression levels of VEGFR-1, and the differences were statistically significant(P<0.05). The serum expression level of miR-139-5p in the AMI group was significantly higher than that in the UAP group(P<0.05), but there was no significant difference in the serum expression level of VEGFR-1 between the two groups(P>0.05). The results of Pearson correlation analysis showed that the serum expression level of miR-139-5p in the ACS patients was negatively correlated with the expression level of VEGFR-1(r=-0.189, P=0.010). The results of Cox regression analysis showed that higher serum expression level of miR-139-5p was a risk factor for MACE development(P<0.05). The results of receiver operating characteristic(ROC) curve analysis showed that the serum expression level of miR-139-5p could effectively predict the occurrence of postoperative MACE ［AUC(95%CI)=0.81(0.73-0.89), P<0.001］, and its optimal cut-off value was 2.08, and the corresponding sensitivity and specificity were 82.83% and 66.28%, respectively. The results of K-M curve analysis showed that the free-MACE survival time of the ACS patients with serum expression level of miR-139-5p≥2.08 was shorter than that of the ACS patients with serum expression level of miR-139-5P<2.08, and the difference was statistically significant(P<0.001). Conclusion　Patients with ACS have a relatively high expression level of serum miR-139-5p, and when the expression level of serum miR-139-5p≥2.08, it indicates that the patients are at a greater risk of developing MACE, which should be concerned by clinicians.]]> 2023/8/4 12:14:58 GAO Yan-lin, CHENG Wei, ZHU Meng-yu, et al. 399true ［Abstract］　Objective　To analyze the correlation of expression levels of long non-coding RNA AC007278.2 and MX dynamin-like GTPase 2(MX2) in the peripheral blood of the systemic lupus erythematosus(SLE) patients with disease activity and complement level. Methods　One hundred and sixty-eight SLE patients(SLE group) admitted to Wuhan Asia General Hospital and Central Command General Hospital of the Chinese People′s Liberation Army from November 2018 to February 2020 were recruited. According to SLE disease activity index-2000(SLEDAI-2000) scores of the patients within 24 hours after admission, 42 patients were inactive, and 45 patients were mildly active, and 42 patients were moderately active, and 39 patients were severely active in disease activity. Eighty healthy people at the same period were recruited as control group. The clinical data of different groups were compared, Pearson correlation analysis was used to explore the correlation between AC007278.2 and MX2 levels and complement C3 and C4 levels as well as disease activity, and multiple linear regression was used to analyze the factors affecting SLE disease activity. Results　The levels of AC007278.2 and MX2 in the SLE group were higher than those in the control group, and the levels of complement C3 and C4, white blood cells(WBC), lymphocytes, platelets(PLT), and hemoglobin(Hb) in the SLE group were lower than those in the control group, and the differences were statistically significant(P<0.05). The results of Pearson correlation analysis showed that AC007278.2 and MX2 levels were negatively correlated with complement C3 and C4 levels in the SLE patients(P<0.05), and were positively correlated with SLEDAI-2000 scores(P<0.05). The results of multiple linear regression analysis showed that AC007278.2(β=0.410) and MX2(β=0.512) were positively correlated with SLE disease activity(P<0.05), while complement C3(β=-0.362) and complement C4(β=-0.528) were negatively correlated with SLE disease activity(P<0.05). Conclusion　The expression levels of AC007278.2 and MX2 in the peripheral blood of SLE patients are elevated and are associated with SLE disease activity and complement C3 and C4.]]> 2023/8/4 0:00:00 FANG Li, YE Ting, LIU Rui 398true ［Abstract］　Objective　To analyze the short-term clinical efficacy of anlotinib combined with programmed death-1(PD-1) inhibitors in treatment of advanced non-small cell lung cancer patients and its effects on serum vascular endothelial growth factor(VEGF) and matrix metalloproteinase-2(MMP-2) levels. Methods　The clinical data of 110 patients with stage ⅢB-Ⅳ non-small cell lung cancer who were admitted to the Third People′s Hospital of Chengdu from February 2018 to February 2022 were selected and divided into combined treatment group(receiving anlotinib combined with PD-1 inhibitors) and anti-PD-1 treatment group(receiving PD-1 inhibitors) according to different treatment methods, with 55 cases in each group. A treatment cycle of 21 days was performed on both groups, and the drugs were withdrawn 7 days after each treatment cycle, and the treatment lasted for 3 cycles. The serum levels of VEGF and MMP-2 were compared between the two groups before treatment, and 1 week, 1 month and 3 months after treatment, and the clinical efficacy at the end of the course of treatment were compared between the two groups. Results　After treatment, the serum levels of VEGF and MMP-2 in the two groups showed a downward trend(P<0.05), and at 1 week, 1 month and 3 months after treatment, the serum levels of VEGF and MMP-2 in the combined treatment group were lower than those in the anti-PD-1 treatment group, and the differences were statistically significant(P<0.05). The objective response rate(ORR) and the disease control rate(DCR) of the combined treatment group were significantly higher than those of the anti-PD-1 treatment group, and the differences were statistically significant(45.45% vs 23.64%, 90.91% vs 76.36%; P<0.05). There was no significant difference in the incidence of total adverse reactions between the two groups(70.91% vs 78.18%; P>0.05). Conclusion　Anlotinib combined with PD-1 inhibitors has significant efficacy in treatment of patients with advanced non-small cell lung cancer, and can reduce their serum VEGF and MMP-2 levels, and the safety of the combination of anlotinib and PD-1 inhibitors is relatively high.]]> 2023/8/4 12:14:58 GAO Hang, YIN Jun, LI Yan-wu 397true ［Abstract］　Objective　To compare the prognostic outcomes between laparoscopic suturing in laparoscopic hysterectomy and transvaginal suturing for vaginal stumps. Methods　The clinical data of 341 patients who underwent hysterectomy in the Department of Gynecology of the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2016 to December 2019 were collected. The patients were divided into transvaginal suturing group(transvaginal group, 98 cases) and laparoscopic suturing group(laparoscopic group, 243 cases) according to different ways to close the vaginal stumps after hysterectomy. The postoperative hospitalization time, pelvic infection, bleeding from the vaginal stumps, urinary system injury, re-suturing, readmission within 30 days after surgery and healing of the vaginal stumps were compared between the two groups. Results　The operations were successfully completed in both groups, and no cases were transferred to open surgery, and no cases were transferred to the Department of Critical Care Medicine for treatment after surgery. There were no significant differences in the operation time and operative blood loss between the two groups(P>0.05). The length of postoperative hospital stay in the laparoscopic group was shorter than that in the transvaginal group, and the readmission rate within 30 days after surgery in the transvaginal group was higher than that in the laparoscopic group, and the differences were statistically significant(P<0.05). The re-suturing rate of bleeding from the vaginal stumps, the pelvic infection rate, the rupture rate of vaginal stumps and the overall incidence of complications in the transvaginal group were significantly higher than those in the laparoscopic group(P<0.05). The proportions of patients with polyps on vaginal stumps in the transvaginal group were higher than those in the laparoscopic group 3 and 6 months after surgery, and the differences were statistically significant(P<0.05). Further analysis showed that postoperative pelvic infection was significantly associated with polyps on vaginal stumps(P<0.05). Conclusion　Laparoscopic suturing for vaginal stumps in laparoscopic hysterectomy is beneficial to reduce the incidence rates of postoperative pelvic infection, rupture of vaginal stumps and polyps on vaginal stumps, which is worthy of clinical recommendation.]]> 2023/8/4 12:14:58 HUANG Wei-rong, FENG Yi-lan, ZHAO Ren-feng 396true ［Abstract］　Objective　To analyze the strategies and clinical effects of postoperative continuous/recurrent reoperation in patients with chronic renal disease secondary hyperparathyroidism(SHPT). Methods　The clinical data of 20 patients with continuous /recurrent SHPT after surgery who were admitted to the First Affiliated Hospital of Guangxi University of Chinese Medicine from January 2013 to November 2021 were retrospectively analyzed. The indications of reoperation of continuous/recurrent SHPT, methods of preoperative imaging localization of parathyroid glands, operative methods, intraoperative determination of intact parathyroid hormone(iPTH) and strategies for exploration and resection of parathyroid glands were summarized. The changes of the indicators including postoperative symptoms, iPTH, blood calcium and blood phosphorus were observed in patients undergoing reoperation. Results　In this study, a total of 32 diseased parathyroid glands were resected in the 20 patients, and the accuracy rate of parathyroid localization reached 93.55%(29/31) through the preoperative radiograph reading analysis with the cooperation of multidisciplinary physicians, and combining with color Doppler ultrasound and computed tomography(CT) scanning. The symptoms of bone pain and skin pruritus disappeared after surgery. The levels of blood iPTH, calcium and phosphorus after operation were significantly decreased compared with those before operation(P<0.05). None of the 20 patients suffered from severe hypocalcemia or other serious complications after surgery, and no continuous/recurrent SHPT occurred during the follow-up. Conclusion　Combining with multiple imaging examinations and joint X-ray film reading through multidisciplinary physicians can improve the accuracy rate of parathyroid location before surgery, and the clinical effects of total parathyroid resection are good in the treatment of continuous/recurrent SHPT.]]> 2023/8/4 12:14:58 LIAO Dan, LIU De-guo, LI Qi-shang 395true ［Abstract］　Objective　To investigate the expressions of von Willebrand factor(vWF) in serum and skin tissues of patients with psoriasis and their clinical significance. Methods　Thirty patients with psoriasis vulgaris who were admitted to Lvliang People′s Hospital from January 2021 to December 2022 were recruited as the psoriasis group, and other 30 surgical patients with normal skin tissues were selected as the control group. The expression levels of the serum vWF in the subjects of the two groups were determined by enzyme-linked immunosorbent assay(ELISA). The expression levels of vWF in the skin tissues of the two groups were detected by immunohistochemical staining. The correlations of the expression levels of serum vWF with the expression intensity of vWF in skin tissues and the psoriasis area and severity index(PASI) scores were analyzed. Results　The expression levels of the serum vWF in the psoriasis group were higher than those in the control group, and the differences were statistically significant(P<0.05). In the epidermis and the inflammatory cells and endothelial cells of the dermis, the expression intensity of vWF in the psoriasis group was higher than that in the control group, and the proportion of moderate and severe expressions in the psoriasis group was greater, and the differences were statistically significant(P<0.05). The results of Pearson correlation analysis showed that the expression levels of the serum vWF in the psoriasis patients were positively correlated with PASI scores(r=0.432; P=0.009). The results of one-way ANOVA analysis showed that there were no significant correlations of the expression levels of the serum vWF in the psoriasis patients with the expression intensities of vWF in the dermis and the epidermis(P>0.05). Conclusion　The expression levels of serum vWF in psoriasis patients are increased, and are positively correlated with PASI scores.]]> 2023/8/4 12:14:58 ZHAO Wen-hua, GUO Ling-na 394true ［Abstract］　Objective　To investigate the expression levels of integrin β4(ITGB4) protein in papillary thyroid carcinoma tissues of elderly patients and their clinical significance. Methods　Eighty elderly patients with papillary thyroid carcinoma who received total thyroidectomy in Haikou Hospital of Traditional Chinese Medicine from August 2019 to August 2020 were recruited, and other 50 patients with nodular goiter who received surgical treatment during the same period were selected as controls. The papillary carcinoma tissues of the thyroid and their para-cancerous tissues, and the nodular goiter lesion tissues were resected during operation and immunohistochemical staining was performed. The ITGB4 protein expression level was detected by Western blot method. The correlations of ITGB4 protein expression level with the clinicopathological features and prognosis of elderly papillary thyroid carcinoma patients were analyzed. Results　The ITGB4 protein was mainly expressed in the cytoplasm. The expression level of ITGB4 protein in the papillary thyroid carcinoma tissues was significantly higher than that in the para-cancerous tissues and the nodular goiter tissues(P<0.05), but there was no significant difference in the expression level of ITGB4 protein between the para-cancerous tissues and the nodular goiter tissues(P>0.05). Compared with those in the patients with TNM stage Ⅰ-Ⅱ, the ITGB4 protein expression levels in the patients with TNM stage Ⅲ -Ⅳ were high. Compared with that in the patients without lymph node metastasis, the ITGB4 protein expression level in the patients with lymph node metastasis was high. Compared with the highly differentiated patients, the medium-differentiated patients and the low-differentiated patients had high ITGB4 protein expression levels, and the expression level in the low-differentiated patients was higher than that in the medium-differentiated patients, and the differences were statistically significant(P<0.05). For the patients with papillary thyroid carcinoma, the recurrence, survival and prognosis of the low ITGB4 expression group were significantly better than those of the high ITGB4 expression group(P<0.05). Conclusion　ITGB4 protein is highly expressed in the elderly patients with papillary thyroid carcinoma, and is significantly correlated with TNM stage, absence of lymph node metastasis, and degree of differentiation, which helps to predict the prognosis of the patients.]]> 2023/8/4 12:14:58 LI Zu-jin, WU Min, SUN Chuan-yan 393true ［Abstract］　Objective　To investigate the correlation between preoperative platelet-to-lymphocyte ratio(PLR) and non-sentinel lymph node(SLN) metastasis in invasive breast cancer. Methods　The clinical data of 280 invasive breast cancer patients with positive sentinel lymph node biopsy who were admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2013 to January 2022 were retrospectively analyzed. The influencing factors of non-SLN metastasis were analyzed by multivariate logistic regression, and the predictive efficacy of PLR for non-SLN metastasis in patients with invasive breast cancer was analyzed by receiver operating characteristic(ROC) curve. Results　The results of ROC curve analysis showed that PLR had the application value of predicting non-SLN metastasis in patients with invasive breast cancer［AUC(95%CI)=0.569(0.508-0.627), P=0.046］, and its optimal cut-off value was 134.85. The results of multivariate logistic regression analysis showed that the number of SLN metastasis more than 1(OR=4.41), vascular infiltration(OR=6.71) and human epidermal growth factor receptor 2(HER2) positive(OR=2.18) were the independent risk factors for non-SLN metastasis(P<0.05). With PLR≤134.85 as reference, the risk of non-SLN metastasis was lower when PLR>134.85(OR=0.56, P<0.05). Conclusion　The level of PLR before treatment is associated with non-SLN metastasis of invasive breast cancer, which should be concerned by clinicians.]]> 2023/7/3 10:09:37 HUANG Kai, WEI Suo-su, HUANG Zhen, et al. 392true ［Abstract］　Objective　To investigate the effect of lactate dehydrogenase(LDH) level on short-term outcomes after vascular replacement surgery in patients with acute type A aortic dissection(ATAAD). Methods　The clinical data of 272 patients with ATAAD who underwent vascular replacement surgery at Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology from October 2020 to March 2022 were selected. The patients were divided into good short-term outcome group(227 cases) and adverse short-term outcome group(45 cases) according to their discharge status. Smooth-fitting curve was used to analyze the relationship between postoperative LDH level and adverse short-term outcomes in the patients. Multivariate logistic regression was used to analyze the factors affecting the adverse short-term outcomes of the patients. Results　The results of multivariate logistic regression analysis showed that higher postoperative LDH level was a risk factor of the adverse short-term prognosis in the patients(P<0.05), and higher postoperative mean blood pressure(MBP) level was a protective factor against adverse short-term outcomes(P<0.05). The smooth-fitting curve showed that postoperative LDH level had a nonlinear relationship with the patients′ adverse short-term outcomes, and the incidence of adverse short-term outcomes increased with the increase of postoperative LDH level. The results of receiver operating characteristic(ROC) curve analysis showed that both postoperative LDH and MBP levels had application value in predicting the short-term outcomes of the ATAAD patients(P<0.05), and combining with the two indicators could further improve the prediction efficiency［AUC(95%CI)=0.752(0.670～ 0.834)］. The sensitivity and specificity were 81.20% and 62.30%, respectively. Conclusion　High postoperative LDH level is a risk factor of adverse short-term outcomes in patients with ATAAD. Monitoring the indicators is helpful for stratifying the risk management of the patients, guiding clinical personalized intervention, and improving the patients′ prognosis.]]> 2023/7/3 10:09:37 HU Shu-xin, ZHOU Hong-min, LI Jun, et al. 391true ［Abstract］　Objective　To analyze the expression levels of sestrin2 mRNA and mammalian target of rapamycin complex 1(mTORC1) mRNA in synovial tissues of patients with osteoarthritis and their correlations with prognosis. Methods　One hundred and twenty patients with osteoarthritis who were admitted to Weifang People′s Hospital from October 2019 to October 2022 were recruited, all of whom underwent knee replacement, and their synovial tissues were collected during the operation. According to the patients′ Lysholm scores 6 months after operation, they were divided into poor prognosis group(<70 points, 31 cases) and good prognosis group(≥70 points, 89 cases). Quantitative real-time polymerase chain reaction(qRT-PCR) was used to detect the levels of sestrin2 mRNA and mTORC1 mRNA in the synovial tissues. The expression levels of joint fluid inflammatory markers［interleukin-8(IL-8), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α)］ were detected using enzyme-linked immunosorbent assay(ELISA) method. Pearson correlation analysis was used to investigate the correlations of sestrin2 mRNA and mTORC1 mRNA levels in the synovial tissues with the inflammatory markers in the articular fluid. Multivariate logistic regression was used to analyze the influencing factors of postoperative poor prognosis in the patients. The efficacy of the sestrin2 mRNA and mTORC1 mRNA levels in the synovial tissues in predicting the prognosis of the osteoarthritis patients was evaluated by receiver operating characteristic(ROC) curve. Results　The levels of mTORC1 mRNA, IL-8, IL-6 and TNF-α in the poor prognosis group were significantly higher than those in the good prognosis group(P<0.05), and the level of sestrin2 mRNA in the poor prognosis group was significantly lower than that in the good prognosis group(P<0.05). The results of Pearson correlation analysis showed that the sestrin2 mRNA level in the synovial tissues of the patients with poor prognosis of osteoarthritis was negatively correlated with IL-8, IL-6 and TNF-α levels in the joint fluid(P<0.05), and the patients′ mTORC1 mRNA level was positively correlated with IL-8, IL-6 and TNF-α levels(P<0.05). The results of multivariate logistic regression analysis showed that the increased expression level of mTORC1 mRNA in the synovium tissues was an independent risk factor for promoting poor prognosis in the osteoarthritis patients(P<0.05), while the higher level of sestrin2 mRNA was a protective factor for inhibiting poor prognosis in the osteoarthritis patients(P<0.05). The results of ROC curve analysis showed that the levels of sestrin2 mRNA and mTORC1 mRNA in the synovial tissues could effectively predict the prognosis of the patients(P<0.05), and the predictive efficacy of the combination of the two indicators was better［AUC(95%CI)=0.906(0.848-0.963), P<0.001］. The sensitivity and specificity were 93.53% and 78.76%, respectively. Conclusion　Sestrin2 mRNA and mTORC1 mRNA in the synovial tissues can be used as important auxiliary indicators for the prognosis evaluation of patients with osteoarthritis.]]> 2023/7/3 10:09:37 FU Dao-tong, LIU Chang-jie, ZHANG Yi-min 390true ［Abstract］　Objective　To observe the medium to long-term clinical efficacy of preserving the left colic artery(LCA) during laparoscopic natural orifice specimen extraction surgery(NOSES) for colorectal cancer. Methods　The clinical data of 155 patients with rectal cancer or sigmoid colon cancer who underwent laparoscopic NOSES in Sichuan Cancer Hospital from July 2016 to December 2021 were retrospectively analyzed. According to whether the LCA was retained or not, the patients were divided into the retained LCA group(80 cases) and the non-retained LCA group(75 cases). The postoperative recurrence, metastasis, and medium to long-term survival were compared between the two groups. Results　The retained LCA group was followed up for 4-65 months, and the non-retained LCA group was followed up for 7-71 months. During the follow-up periods, there were 4 cases(5.1%) of local recurrence or distant metastasis in the retained LCA group and 7 cases(9.5%) of local recurrence or distant metastasis in the non-retained LCA group. There was no significant difference in disease-free survival(DFS) between the two groups(P>0.05). Further stratified analysis was conducted using TNM staging, and there was no statistically significant difference in DFS between the two groups of stage Ⅰ, Ⅱ and Ⅲ patients(P>0.05). There were 2 deaths(2.5%) in the retained LCA group and 2 deaths(2.7%) in the non-retained LCA group. There was no significant difference in overall survival(OS) between the two groups(P>0.05). The results of the further stratified analysis using TNM staging showed that there was no statistically significant difference in OS between the two groups of stage Ⅰ, Ⅱ and Ⅲ patients(P>0.05). Conclusion　Retaining the LCA while completely removing the root lymph nodes of the inferior mesenteric artery does not affect the patients′ medium to long-term oncologic outcomes, as long as the surgical oncologic principles are strictly followed during the laparoscopic NOSES for colorectal cancer.]]> 2023/7/3 10:09:37 FU Chuan-chuan, ZHAO Bin, YI Bo, et al. 389true ［Abstract］　Objective　To investigate the efficacy of cognitive behavioral therapy(CBT) combined with escitalopram in alleviating postoperative anxiety in elderly patients with colorectal cancer and its effect on the occurrence of postoperative delirium(POD). Methods　A total of 110 elderly patients who received laparoscopic radical resection of colorectal cancer in General Hospital of Huainan Oriental Hospital Group from November 2021 to August 2022 were recruited and divided into observation group and control group by random number table method, with 55 cases in each group. The patients in the control group were given escitalopram from the day of admission to the day before surgery, and the observation group received CBT plus the same treatment as the control group. The incidence of POD within three days after surgery was the main observation indicator. The secondary observation indicators included the following: the Hamilton Anxiety Scale(HAMA) scores and Mini-Mental State Examination(MMSE) scores on the day of admission, 1 day before surgery, and 1, 3 and 7 days after surgery; the Numerical Rating Scale(NRS) scores and Ramsay sedation scores within 48 hours after surgery; the incidence of postoperative adverse events; 15-Item Quality of Recovery Questionnaire(QoR-15) scores 1 and 2 days after surgery. The off-bed ambulation time and the length of hospital stay were recorded in the patients. Results　Compared with those on the day of admission, the MMSE scores and HAMA scores in the two groups showed a downward trend, and the HAMA scores of the observation group decreased more than those of the control group, and the HAMA scores of the observation group were lower than those of the control group 1 day before surgery, and 1, 3 and 7 days after surgery, and the differences were statistically significant(P<0.05). There was no significant difference in the range of MMSE scores between the two groups(P>0.05). The postoperative NRS scores showed a downward trend, and the Ramsay sedation scores showed an upward trend in the two groups, but there were no statistically significant differences in the ranges of the changes between the two groups(P>0.05). Within 3 days after surgery, the incidence of POD in the observation group was significantly lower than that in the control group(10.91% vs 30.91%; χ²=6.652, P=0.010), and there were no significant differences in POD severity score and POD duration between the two groups(P>0.05). The QoR-15 scores of the observation group were significantly higher than those of the control group on the first day and the second day after surgery(P<0.05). Compared with the control group, the observation group had earlier off-bed ambulation time, and the difference was statistically significant(P<0.05). Conclusion　CBT combined with escitalopram can reduce perioperative anxiety in elderly patients undergoing laparoscopic radical resection of colorectal cancer, reduce the incidence of POD, and facilitate rapid recovery of the patients.]]> 2023/7/3 10:09:37 XUE Ling, ZOU Xu, XIA Ji-chang, et al. 388true ［Abstract］　Objective　To investigate the correlation between root curvature of impacted anterior maxillary teeth and labial and palatal bone cortex based on cone beam computed tomography(CBCT). Methods　The clinical data of 71 patients with impacted anterior maxillary teeth who were admitted to the Department of Orthodontics of Affiliated Stomatology Hospital of Guizhou Medical University from January 2018 to December 2022 were collected, aged 7-13 years old, including 39 impacted canines and 32 impacted incisors. All the patients were treated with closed intermittent light traction. According to Nolla′s method, the impacted incisors were divided into early tooth age group(10 teeth) and late tooth age group(10 teeth). The Dolphin software was used to measure the length of the root, the curvature of the root, the distance between the root tip and the labial bone cortex, and the distance between the root tip and the palatine bone cortex. Pearson correlation analysis was used to explore the correlation between the indexes. Results　There was no significant correlation between the angle of the root and the distance from the root tip to the labial-palatine cortex before corrective treatment(P>0.05). The angle of the incisor root was positively correlated with the distance from the apical tip to the palatal cortex(P<0.05), and negatively correlated with the distance from the apical tip to the labial cortex(P<0.05). After corrective treatment, the root length and root angle of the incisors were greater than those before corrective treatment, the differences were statistically significant(P<0.05). The changes of the root angle were negatively correlated with the changes of the distance between the root tip and the labial cortex(r=-0.690, P=0.001), and were positively correlated with the distance between the root tip and the palatal cortex(r=0.645, P=0.015) before and after the treatment of the incisor teeth. The root angle and root length of early incisor teeth after corrective treatment were significantly greater than those before treatment(P<0.05), but there were no statistically significant differences in the root angle and root length of late incisor teeth after corrective treatment(P>0.05). Conclusion　Root curvature and root length of the impacted incisors are correlated with the distance from the root tip to the labial and palatal bone cortices.]]> 2023/7/3 10:09:37 TANG Yun, GAO Juan, PENG Ju-xiang, et al. 387true ［Abstract］　Objective　To analyse the correlation between eukaryotic translation initiation factor 4E family member 3(eIF4E3) and the prognosis of hepatocellular carcinoma(HCC) patients and to explore its mechanisms of action. Methods　The expression differences of eIF4E3 in HCC and normal liver tissues were analyzed by using Tumor Immune Estimate Resource(TIMER) database, Gene Set Cancer Analysis(GSCA) database, Hepatocellular Carcinoma Database(HCCDB) and University of Alabama at Birmingham Cancer Data Analysis Portal(UALCAN) database, and the relationship between the transcription level of eIF4E3 and the clinicopathological characteristics of HCC patients was analyzed by using UALCAN database. The GSCA database and Kaplan-Meier Plotter database were used for survival analysis. The Linked Omics database was used to explore the co-expression genes of eIF4E3, and the co-expressed genes were analyzed by using Gene Ontology(GO) and the Kyoto Encyclopedia of Genes and Genomes(KEGG). Finally, the Tumor-Immune System Interactions Database(TISIDB) and the Sanger Box platform were used to analyze the relationship between eIF4E3 and immune invasion in the tumor microenvironment. Results　The expression level of eIF4E3 in the HCC tissues was significantly lower than that in the normal liver tissues(P<0.05). In the HCC patients, the overall survival(OS), disease-free survival(DFS), progression-free survival(PFS) and disease-free interval(DFI) in the low eIF4E3 expression group were significantly shorter than those in the high eIF4E3 expression group(P<0.05). The results of functional enrichment analysis showed that the function of eIF4E3 was mainly enriched in cell adhesion, cell cycle and immune response(P<0.05). The expression level of eIF4E3 was significantly negatively correlated with the infiltration abundances of naive CD4⁺ T cells, resting natural killer(NK) cells, monocytes, resting mast cells and eosinophils(P<0.05), and positively correlated with the infiltration abundances of resting CD4 memory T cells, activated CD4 memory T cells, M1 macrophages, activated dendritic cells, resting dendritic cells and neutrophils(P<0.05). Conclusion　eIF4E3 is a potential tumor suppressor gene, which may affect the prognosis of patients by affecting the immune invasion status in the HCC tumor microenvironment, and has the application prospect as a target of HCC immunotherapy.]]> 2023/7/3 10:09:37 ZU Ling, LI Wan-li, LIU Tian-qi 386true ［Abstract］　Objective　To compare the clinical effects of 561 nm and 532 nm laser photocoagulations on treatment of severe non-proliferative diabetic retinopathy(NPDR). Methods　Forty-six patients(82 eyes) with severe NPDR without macular edema who were admitted to Huizhou Third People′s Hospital from June 2021 to August 2022 were recruited. All of them were treated with panretinal photocoagulation(PRP). The patients were divided into observation group(23 cases, 42 eyes) and control group(23 cases, 40 eyes) by random number table method. The treatment wavelength of laser photocoagulation was 561 nm in the observation group and 532 nm in the control group. The best corrected visual acuity(BCVA), central macular thickness(CMT) and retinal nerve fibre layer(RNFL) thickness were compared between the two groups before treatment and 2 weeks, 1 month, 3 months and 6 months after treatment. The occurrence of adverse reactions was compared between the two groups. Results　After treatment, the values of BCVA in the two groups were significantly increased compared with those before treatment(P<0.05), but there was no significant difference in BCVA at each time point between the two groups(P>0.05). After treatment, the thicknesses of CMT and RNFL showed a downward trend in the affected eyes in both groups(P<0.05), and the thicknesses of CMT in the observation group were lower than those in the control group 2 weeks, 1 month, 3 months and 6 months after treatment, and the thicknesses of RNFL in the observation group were higher than those in the control group, with statistically significant differences between the two groups(P<0.05). There were no significant differences in the incidence rates of secondary macular edema, vitreous hematoma and neovascular glaucoma between the two groups after treatment(P>0.05). Conclusion　In the treatment of severe NPDR, 561 nm laser photocoagulation is safe and effective, and has less effects on the thicknesses of CMT and RNFL than 532 nm laser, which is beneficial to the preservation of visual function.]]> 2023/7/3 10:09:37 DUAN Na, LI Jia-qing, CHEN Meng-ping, et al. 385true ［Abstract］　Objective　To analyze the efficacy of exercise stress echocardiographic indicators combined with serum soluble suppression of tumorigenesis 2(sST2)/left atrial volume index(LAVI) ratio in diagnosis of diastolic heart failure(DHF). Methods　One hundred and twenty patients with chronic heart failure who were admitted to Shengli Oilfield Central Hospital from October 2020 to October 2022 were recruited and divided into DHF group(53 cases) and non-DHF group(67 cases) according to the occurrence of DHF. All the patients underwent exercise load echocardiography to obtain the ratio of LAVI, mitral annulus diastolic velocity(E) to mitral annulus mean early diastolic velocity(e′) ratio, and the variations of E/e ′ under load and at rest(△E/e′) were calculated. The serum levels of sST2 were detected and sST2/LAVI ratio was calculated. Pearson correlation was used to analyze the correlation of echocardiographic indicators and serum levels of sST2 with left ventricular end diastolic pressure(LVEDP) and pulmonary capillary wedge pressure(PCWP). Receiver operating characteristic(ROC) curve analysis was used to study the diagnostic efficacy of DHF. Results　The E/e′ ratio under load in the DHF group was significantly higher than that at rest(P<0.05), but there was no significant difference in the E/e ′ ratio between resting and load states in the non-DHF group(P>0.05). The resting state E/e′ ratio, load state E/e′ ratio, △E/e′ ratio and LAVI level in the DHF group were higher than those in the non-DHF group, while sST2 level and sST2/ LAVI ratio in the DHF group were lower than those in the non-DHF group, and the differences were statistically significant(P<0.05). The results of Pearson correlation analysis showed that resting E/e′ ratio, load E/e′ ratio, △E/e′ ratio and LAVI were positively correlated with PCWP and LVEDP(P<0.05), while sST2 and sST2/ LAVI ratio were negatively correlated with PCWP and LVEDP(P<0.05). The results of ROC curve analysis showed that the △E/e′ ratio had application value in diagnosing DHF［AUC(95%CI)=0.837(0.758-0.898), P<0.05］, and the optimal cut-off value was 1.50, and the corresponding sensitivity and specificity were 83.02% and 83.58%, respectively. The ratio of sST2/ LAVI also had application value in the diagnosis of DHF［AUC(95%CI)=0.804(0.722-0.871), P<0.05］, and the optimal cut-off value was 0.75, and the corresponding sensitivity and specificity were 81.13% and 80.60%, respectively. Combining E/e′ ratio and sST2/ LAVI ratio could further improve the diagnostic efficacy［AUC(95%CI)=0.950(0.895-0.982), P<0.05］, and the sensitivity and specificity were 94.34% and 95.52%, respectively. Conclusion　Compared with the non-DHF patients, the DHF patients have elevated ratio of △E/e′ and decreased ratio of sST2/LAVI, which is strongly correlated with both LVEDP and PCWP. The combination of △E/e′ ratio and sST2/LAVI ratio can provide auxiliary reference for clinical diagnosis of DHF.]]> 2023/7/3 10:09:37 WANG Wei, YAN Ping, LU Rong, et al. 384true ［Abstract］　Objective　To establish the normal reference range of thyroid stimulating hormone(TSH) for healthy adults in Jinchengjiang District of Hechi City. Methods　Two hundred and seventy-three healthy adults who underwent physical examination in Hechi Traditional Chinese Medicine Hospital from January 2015 to August 2022 were selected, and TSH detection was performed on them. The range of normal reference values was determined in the 2.5 to 97.5 percentile range(P_2.5 to P_97.5). Results　The normal reference range of TSH for healthy adults in Jinchengjiang District of Hechi City was (0.48-4.26)μIU/ml, which was similar to the normal reference range (0.4-4.5 μIU/ml) provided by the kit manufacturer, but the range of the former was more narrow. The normal reference values of TSH for males ranged from 0.49 to 4.12 μIU/ml, while the normal reference values of TSH for females ranged from 0.47 to 4.09 μIU/ml. The difference in TSH levels was statistically significant between the males and the females(Z=2.296, P=0.022). There was no significant correlation between the normal reference range of TSH and age(P>0.05). Conclusion　The normal reference range of TSH in healthy adults in Jinchengjiang District of Hechi City can be used as a regional reference standard for healthy adults in Hechi City, which is conducive to improving the accuracy of diagnosis for local thyroid diseases.]]> 2023/7/3 10:09:37 WU Kun, MENG En-ji, QIN Yu-qi, et al. 383true ［Abstract］　Objective　To understand the difference in depression tendency between the children with congenital malformations of external and middle ear, and the normal children of the same age, and to analyse the correlation of the results between the pediatric patients′ Marx microtia dysmorphia classification as well as the stage of operation and the degree of depression. Methods　Seventy-nine children with congenital malformations of external and middle ear who were treated in the inpatient and outpatient departments of Sun Yat-sen Memorial Hospital, Sun Yat-sen University from June 2020 to April 2021 were selected as the disease group, and 30 normal children of the same age were selected as the control group. Zung Self-rating Depression Scale(SDS) was used to analyze the test results of the two groups, and the correlation between the pediatric patients′ Marx microtia dysmorphia classification as well as the stage of operation and the degree of depression. Results　Forty-two children with congenital malformations of external and middle ear had different degrees of depression, and the difference in depression between the disease group and the control group was statistically significant(Z=2.762, P=0.006). There was no significant correlation between depression and age(r=0.102, P=0.293). The degree of depression was correlated with Marx microtia dysmorphia classification(r=0.231, P=0.040). There was no significant difference in the degree of depression among the groups at different surgical stages(H=5.103, P=0.164). Conclusion　Compared with normal children, children with congenital malformations of external and middle ear have significant different proportion of depression tendency. More importantly, the higher the degree of microtia dysmorphia, the more severe the depression. The results show the necessity for children with congenital malformation of external and middle ear to take the treatment of total ear reconstruction operation and the necessity of paying attention to the psychological problems of such children at an early stage.]]> 2023/5/31 10:09:27 WENG Jin, XU Jia-jie, SUN Yi, et al. 382true ［Abstract］　Objective　To analyse the survival prognosis after radical hepatectomy in non-hepatitis B associated with hepatocellular carcinoma(HCC) patients by using modified albumin-bilirubin(mALBI) grading. Methods　The clinical data of 130 non-hepatitis B associated with HCC patients who received radical hepatectomy in the People′s Hospital of Guangxi Zhuang Autonomous Region from March 2013 to March 2022 were collected. According to the results of mALBI scores, they were divided into mALBI 1 grade, mALBI 2a grade, mALBI 2b grade and mALBI 3 grade. The clinical data were compared among the patients with different grades. Cox regression was used to analyze the factors affecting the survival prognosis of the non-hepatitis B associated with HCC patients, and Kaplan-Meier survival curve was used to analyze the difference in survival prognosis among the patients with different mALBI grades. Results　Among the 130 non-hepatitis B associated with HCC patients in this study, 48 cases(36.92%) were mALBI 1 grade, and 39 cases(30.00%) were mALBI 2a grade, and 43 cases(33.08%) were mALBI 2b grade, and 0 cases(0.00%) were mALBI 3 grade. During the follow-up period, 4 cases(8.33%) of mALBI 1 grade died, and 5 cases(12.82%) of mALBI 2a grade died, and 13 cases(30.23%) of mALBI 2b grade died. The survival prognosis in the patients with mALBI 1 grade was significantly better than that in the patients with mALBI 2a grade and in the patients with mALBI 2b grade(log-rank test: χ²=13.560, P=0.005). The results of Cox regression analysis showed that tumor diameter ≥5 cm(aHR=4.710), microvascular invasion(MVI)(aHR=3.460), and higher mALBI scores(aHR=4.990) were independent risk factors for the survival prognosis of the non-hepatitis B associated with HCC patients(P<0.05). Conclusion　mALBI grading can be used to predict the survival prognosis of non-hepatitis B associated with HCC patients after radical hepatectomy, which is conducive to early clinical intervention and improvement of the patients′ prognosis.]]> 2023/5/31 10:09:27 LIU Hong-jun, DONG Xiao-feng, HUANG Wei, et al. 381true ［Abstract］　Objective　To analyze the factors affecting the survival prognosis of the patients with gallbladder cancer(GBC) after radical resection, and to construct a nomogram model for survival prediction. Methods　The clinical data of 1 056 patients who underwent GBC radical resection from 2006 to 2015 were extracted from the SEER database(https://seer.cancer.gov/data-software/) as the training group. The clinical data of 76 patients undergoing GBC radical resection in Anhui Provincial Hospital Affiliated to Anhui Medical University from 2015 to 2020 were collected as the validation group. Based on the data of the training group, Cox regression was used to analyze the factors affecting the survival prognosis of the GBC patients after radical resection, and the nomogram model was constructed based on the analysis. The Bootstrap method was used for self sampling 1 000 times to verify the model internally, and then the validation set data was used to verify the model externally. Results　The results of Cox regression analysis showed that age, tumor diameter, number of lymph nodes dissected, TNM stage, T stage and N stage were the independent factors affecting the survival of the GBC patients after radical resection(P<0.05). Based on the results, the nomogram model was constructed to predict the 1-year, 2-year, and 3-year overall survival rates of the GBC patients after radical resection. The internal verification showed C-index(95%CI)=0.740(0.720-0.760), and the external verification showed C-index(95%CI)=0.707(0.627-0.787). Correction curve showed high consistency between the model prediction and the actual survival situation. Conclusion　The nomogram model constructed based on SEER database has high accuracy and stability in predicting the 1-year, 2-year, and 3-year overall survival rates of the GBC patients after radical resection, which can be used to guide clinical practice.]]> 2023/5/31 10:09:27 WEI Zhen-kang, SHAO Feng, HUANG Qiang 380true ［Abstract］　Objective　To investigate the efficacy of imatinib combined with recombinant human interferon α-2b(IFNα-2b) in the treatment of advanced chronic myeloid leukemia(CML) and its impacts on serum levels of matrix metalloproteinase-2(MMP-2), spalt-like transcription factor 4(SALL4) mRNA and α1-acid glycoprotein(AGP). Methods　Ninety inpatients with advanced CML who were admitted to Shaanxi Provincial People′s Hospital from January 2017 to January 2021 were recruited. The patients were divided into the observation group(treated with imatinib combined with IFNα-2b, 45 cases) and the control group(treated with imatinib, 45 cases). The clinical efficacy, and serum levels of MMP-2, SALL4 mRNA and AGP, and the occurrence of adverse reactions were compared between the two groups. Results　The proportion of the patients who achieved complete hematological remission(CHR) after treatment in the observation group was higher than that in the control group, and the overall efficacy in the observation group was better, and the differences were statistically significant(P<0.05). There were no significant differences in the serum levels of interleukin-6(IL-6), prostaglandin E₂(PGE₂), alkaline phosphatase(ALP), MMP-2, SALL4 mRNA and AGP between the two groups before treatment(P>0.05). After treatment, the levels of IL-6, PGE₂, ALP, MMP-2, SALL4 mRNA and AGP were significantly decreased compared with those before treatment in the two groups(P<0.05), and the levels of the observation group after treatment were lower than those of the control group, and the differences were statistically significant(P<0.05). The incidence of hematological adverse reactions in the observation group was higher than that in the control group［22.22%(10/45) vs 6.67%(3/45); χ²=4.406, P=0.039］. There was no significant difference in the incidence of non-hematological adverse reactions［6.67%(3/45) vs 4.44%(2/45); χ²=0.000, P=1.000］ and there was significant difference in the total incidence of adverse reactions［28.89%(13/45) vs 11.11%(5/45); χ²=4.444, P=0.035］. Conclusion　Imatinib combined with IFNα-2b is more effective than imatinib alone in the treatment of advanced CML, and can reduce serum levels of MMP-2, SALL4 mRNA and AGP. However, imatinib combined with IFNα-2b may increase the risk of hematological adverse reactions.]]> 2023/5/31 10:09:27 WANG Hui, LI Yan-chun, GAO Qiu-ying, et al. 379true ［Abstract］　Objective　To investigate the efficacy of prednisone combined with azithromycin sequential therapy in treatment of severe Mycoplasma pneumoniae pneumonia in children and its effects on serum Toll-like receptor 4(TLR4)/myeloid differentiation factor 88(MyD88)/nuclear factor kappa-B(NF-κB) signaling pathway related proteins and downstream inflammatory factors. Methods　One hundred and twenty children with severe Mycoplasma pneumoniae pneumonia who were admitted to Tangshan Maternal and Child Health Hospital from January 2019 to June 2022 were recruited and divided into the control group(treated with azithromycin, 60 cases) and the observation group(treated with prednisone combined with azithromycin, 60 cases) by random number table method. After 4 weeks of treatment, the therapeutic effects, Clinical Pulmonary Infection Scale(CIPS) scores, levels of TLR4/MyD88/NF-κB signaling pathway related proteins and downstream inflammatory factors were compared between the two groups. Results　The temperature recovery time, cough relief time, sputum relief time, wheezing relief time and lung rales disappearance time of the patients in the observation group was faster than that in the control group, and the length of hospital stay in the observation group was shorter, with statistically significant differences between the two groups(P<0.05). After treatment, the scores of CIPS items such as body temperature, white blood cell count, airway secretions, oxygenation, chest X-ray and sputum culture, and total scores in both groups were decreased compared with those before treatment, and the scores in the observation group were lower than those in the control group, and the differences were statistically significant(P<0.05). After treatment, the levels of TLR4, MyD88, NF-κB, C-reactive protein(CRP), interleukin-6(IL-6) and procalcitonin(PCT) in the two groups were decreased compared with those before treatment, and the above levels in the observation group were lower than those in the control group, and the differences were statistically significant(P<0.05). Conclusion　Prednisone combined with azithromycin sequential therapy can significantly down-regulate the levels of serum TLR4/MyD88/NF-κB signaling pathway related proteins and downstream inflammatory factors, which can improve the clinical therapeutic effect of severe Mycoplasma pneumoniae pneumonia in children.]]> 2023/5/31 10:09:27 YAN Hui, WANG Lan-ying, WU Xiao-lei, et al. 378true ［Abstract］　Objective　To explore the mechanism of Cnidii fructus-Psoralea fructus in treatment of bone metastasis of breast cancer based on network pharmacology. Methods　The chemical components of drugs were retrieved by using Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP) and Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine(BATMAN-TCM), while the corresponding targets of the chemical components were obtained by the Swiss Target Prediction Database. The targets related to bone metastasis of breast cancer were retrieved in GeneCards, and Online Mendelian Inheritance in Man(OMIM) Database. Venny 2.1 online software was used to obtain the common targets of drugs-disease, and then the “drug-compound-target-disease” network diagram was constructed by using the software Cytoscape 3.8.2. The STRING database was used to draw the protein-protein interaction network, and the GO function enrichment and KEGG pathway enrichment analysis were carried out through the DAVID database. Results　Forty active components of Cnidii fructus-Psoralea fructus could regulate 99 targets and 147 pathways to treat bone metastasis of breast cancer. Diosmetin, (E)-2, 3-bis(2-keto-7-methoxy-chromen-8-yl) acrolein, isobavachin, cniforin B, cniforin A, O-isovalerylcolum bianetin, O-acetylcolumbianetin, bavachinin, xanthoxylin N, O-isovalyldihydrocarvinol, ostruthin and osthol could mediate pathways in cancer, endocrine resistance, phosphatidylinositol 3-kinase-serine/threonine kinase(PI3K-Akt), proteoglycans in cancer, microRNAs in cancer, central carbon metabolism in cancer, prolactin, cellular senescence, apoptosis, advanced glycation end products-receptor for advanced glycation end products(AGE-RAGE) signaling pathway in diabetic complications, Ras, mitogen-activated protein kinase(MAPK) and other signaling pathways through epidermal growth factor receptor(EGFR), RAC-alpha serine/threonine protein kinase(Akt1), steroid receptor coactivator(SRC) and other key target proteins to treat the bone metastasis of breast cancer. Conclusion　Cnidii fructus-Psoralea fructus can participate in the treatment of bone metastasis of breast cancer through multiple components, targets and pathways, which provides a theoretical basis for the clinical application of Cnidii fructus-Psoralea fructus and the mechanism of its treatment of bone metastasis of breast cancer.]]> 2023/5/31 10:09:27 KE Chang-hu, YAN Hui, CHEN Qin-hua, et al. 377true ［Abstract］　Objective　To compare the effects of different suture methods on residual ovarian reserve function in patients undergoing laparoscopic ovarian cystectomy. Methods　One hundred and twenty patients with ovarian endometriosis cyst who were admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region from April 2017 to October 2019 were selected and randomly divided into intracapsular multi-point continuous suture group(group A), base continuous U-shaped suture group(group B), extracapsular continuous suture group(group C), extracapsular interrupted suture group(group D), with 30 cases in each group. The operation time and intraoperative blood loss were compared among the four groups. The ovarian reserve function-related indicators［including serum estradiol(E₂), progesterone(P), follicle stimulating hormone(FSH), luteinizing hormone(LH), antral follicle count(F0), inter-ovarian peak systolic velocity(PSV), end diastolic velocity(EDV), resistance index(RI) and pulse index(PI)］ were compared among the four groups before surgery(T₁), 1 month after surgery(T₂), 3 months after surgery(T₃), 6 months after surgery(T₄), and 1 year after surgery(T₅). Results　The operation time of the group B was the shortest, and its intraoperative blood loss was the least among the four groups, and the difference in the operation time and intraoperative blood loss were statistically significant between the group B and the other three groups(P<0.05). From T₂ to T₅ time points, the levels of E₂, P, F0 and PSV in the four groups showed a decreasing trend first and then increased, reaching the lowest values at T₃ time point. The levels of E₂, P, F0 and PSV in the group B were higher than those in the other three groups, and the amplitude differences were statistically significant among the four groups(P<0.05). The levels of FSH, LH, RI, and PI in the four groups showed an increasing trend first and then decreased, reaching the highest values at T₃ time point. The levels of FSH, LH, RI and PI in the group B were lower than those in the other three groups, and the amplitude differences were statistically significant among the four groups(P<0.05). Conclusion　The base continuous U-shaped suture method has the advantages of good hemostatic effect, short operation time and quick recovery of ovarian reserve function after surgery in patients undergoing laparoscopic ovarian cystectomy.]]> 2023/5/31 10:09:27 LIU Yuan-yuan, ZHAO Ren-feng 376true ［Abstract］　Objective　To investigate the application efficacy of acute gastrointestinal injury(AGI) score, triglyceride(TG) combined with high sensitive C-reactive protein/albumin(hs-CRP/Alb) ratio to assess disease severity and recurrence in patients with hypertriglyceridemic acute pancreatitis(HTG-AP). Methods　Eighty-nine HTG-AP patients admitted to Mianyang Hospital Affiliated to School of Medicine, University of Electronic Science and Technology from June 2019 to June 2022 were recruited. Among them, 32 cases suffered from mild acute pancreatitis(MAP)(the MAP group), and 38 cases suffered from moderate to severe acute pancreatitis(MSAP)(the MSAP group) and 19 cases suffered from severe acute pancreatitis(SAP)(the SAP group). The patients were followed up to observe the disease recurrence within 3 months after discharge from the hospital. Multivariate Cox regression analysis was used to explore the factors affecting disease recurrence. Receiver operating characteristic(ROC) curve was used to evaluate the efficacy of AGI, TG combined with hs-CRP/Alb ratio in predicting disease recurrence. Results　The levels of AGI scores, hs-CRP and hs-CRP/Alb ratio in the SAP group were significantly higher than those in the MSAP group and the MAP group(P<0.05), and the Alb level in the SAP group was significantly lower than that in the MSAP group and the MAP group(P<0.05). The levels of AGI scores, hs-CRP and hs-CRP/Alb ratio in the MSAP group were significantly higher than those in the MAP group(P<0.05), while the Alb level in the MSAP group was significantly lower than that in the MAP group(P<0.05). Of the 89 patients, 5 cases were lost to follow up, and 29 cases relapsed during the follow-up, and 55 cases did not relapse. The results of multivariate Cox regression analysis showed that higher levels of AGI scores, TG and hs-CRP/Alb ratio were the independent risk factors of disease recurrence in the HTG-AP patients(P<0.05). The results of ROC curve analysis showed that TG, AGI scores and hs-CRP/Alb ratio all had application value in predicting disease recurrence in the HTG-AP patients(P<0.05), and the combination of the three indicators could further improve the predicting efficacy［AUC(95%CI)=0.965(0.916-1.000), P<0.05］. Conclusion　The AGI score and the increased hs-CRP/Alb ratio are correlated with the severity of HTG-AP. TG and AGI score combined with hs-CRP/Alb ratio can be used to assess the risk of disease recurrence in the HTG-AP patients.]]> 2023/5/31 10:09:27 LIU Cui-hua, LIU Ji-ning, SHENG Jie, et al. 375true ［Abstract］　Objective　To analyze the effect of uniportal-video-assisted thoracoscopic surgery(VATS) on simultaneous treatment of multiple pulmonary nodules in both lungs. Methods　The clinical data of 24 patients with multiple pulmonary nodules in both lungs who were treated with single-port thoracoscopic lobectomy/sublobectomy in the Department of Cardiothoracic Surgery of Shandong Dongying Hospital of Traditional Chinese Medicine(Dongying Shengli Hospital) from January 2020 to December 2021 were retrospectively analyzed, among whom there were 15 males and 9 females, and their age ranged from 51 to 79 (64.5±8.4)years. One lung lobectomy plus one sublobectomy was performed in 3 cases, and bilateral sublobectomy were performed in 21 cases. Results　All the patients underwent successful surgery and were discharged from the hospital. The operation time was 90-254(162.1±50.9)minutes, and the postoperative hospitalization time was 4-13(7.1±2.3)days, and the time of placing postoperative thoracic drainage tube was 2-4(2.8±0.8)days. The postoperative complications included 2 cases of pulmonary infection, 1 case of poor wound healing, 4 cases of cough discomfort and 4 cases of postoperative dyspnea discomfort. Conclusion　It is feasible to treat multiple pulmonary nodules simultaneously in both lungs with single-pore thoracoscope and the therapeutic effect is satisfactory.]]> 2023/5/31 10:09:27 YUAN Xiao-long, YAN Yun-long, LIU Jian, et al. 374true ［Abstract］　Objective　To analyze the risk factors of Mycoplasma pneumoniae necrotizing pneumonia(MPNP) in children. Methods　The clinical data of 151 children with severe Mycoplasma pneumoniae pneumonia(SMPP) who were admitted to the Respiratory Department of Shanxi Children′s Hospital from January 2021 to December 2022 were collected. According to the results of chest computed tomography(CT) reexamination after treatment, the patients were divided into MPNP group(50 cases) and non-MPNP group(101 cases). Multivariate logistic regression was used to analyze the risk factors of MPNP. Results　The duration of the disease before admission, fever time and hospital stay in the MPNP group were longer than those in the non-MPNP group, and the differences were statistically significant(P<0.05). The levels of white blood cells(WBC), neutrophils, C-reactive protein(CRP), erythrocyte sedimentation rate(ESR), procalcitonin(PCT), alanine aminotransferase(ALT), aspartate aminotransferase(AST), lactate dehydrogenase(LDH) and D-dimer in the MPNP group were higher than those in the non-MPNP group, while the levels of total protein(TP), albumin(ALB) and prealbumin(PA) in the MPNP group were lower than those in the non-MPNP group, and the differences were statistically significant(P<0.05). The proportion of the patients with pleural effusion, pleural thickening and rice-water-like bronchoalveolar lavage fluid(BALF), and the proportion of the patients receiving other antibiotics and low molecular weight heparin calcium in the MPNP group were higher than those in the non-MPNP group, and the differences were statistically significant(P<0.05). The results of multivariate logistic regression analysis showed that higher levels of WBC, D-dimer and rice-water-like BALF were independent risk factors for developing MPNP in the children(P<0.05), and higher levels of PA were protective factors for inhibiting MPNP in the children(P<0.05). The results of receiver operating characteristic(ROC) curve analysis showed that WBC, D-dimer and PA all had diagnostic value for MPNP in the children(P<0.05), and the combination of the three indicators could further improve the diagnostic efficiency［AUC(95%CI)=0.932(0.886-0.979)］. The sensitivity and the specificity were 0.920 and 0.851, respectively. Conclusion　WBC, D-dimer, PA and rice-water-like BALF are factors affecting the occurrence of MPNP. Clinicians should pay close attention to the changes of these indicators. For the children suspected to be complicated with necrotizing pneumonia, the relevant examinations should be further improved to clarify the children′s conditions, and early intervention should be carried out to improve the prognosis of the children.]]> 2023/5/31 10:09:27 WANG Zhi-long, HAN Zhi-ying, WANG Wei 373true ［Abstract］　Objective　To investigate the effects of Garcinone E extracted from Mangostema mangostema peel on the migration and invasion of nasopharyngeal carcinoma S18 cells and their related mechanisms. Methods　Different concentrations of Garcinone E were used to treat S18 cells. The cell viability was detected by CCK-8 method, and the migration and invasion ability of S18 cells were detected by Transwell assay. Western blot was used to detect the expression levels of transfer-related proteins［tissue inhibitor of metalloproteinase 1(TIMP1), tissue inhibitor of metalloproteinase 2(TIMP2), matrix metallopeptidase 2(MMP-2), matrix metallopeptidase 9(MMP-9)］, cyclin-related proteins［cyclin-dependent kinase inhibitor 1A(p21), cyclin-dependent kinase 2(CDK2), cyclin-dependent kinase 6(CDK6), cyclin-dependent kinase 7(CDK7), cyclin B1］ and autophagy-related proteins［microtubule-associated protein 1 light chain 3 beta(LC3B), ubiquitin-binding protein p62(SQSTM1/p62), beclin-1, autophagy-related 3(Atg3)］ in S18 cells. Results　Garcinone E could significantly inhibit the proliferation of S18 cells(P<0.05), and its fifty percent inhibitory concentration(IC₅₀)=(3.34±0.03)μmol/L, and could inhibit the migration and invasion ability of S18 cells(P<0.05). Garcinone E could up-regulate the expression levels of TIMP1, TIMP2, p21, LC3B, SQSTM1/p62, beclin-1 and Atg3 proteins in S18 cells(P<0.05), and could down-regulate the expression levels of MMP-2, MMP-9, CDK2, CDK6, CDK7 and cyclin B1(P<0.05). Conclusion　Garcinone E can inhibit the metastasis and invasion ability of nasopharyngeal carcinoma S18 cells, block cell cycle and inhibit autophagy, and can be used as a candidate lead compound for the development of chemotherapy drug for nasopharyngeal carcinoma.]]> 2023/4/30 11:53:10 WEI Dan, ZHANG Han, YIN Fu-qiang, et al. 372true ［Abstract］　Objective　To analyze the changes of gut microbiota and to analyze its metabolic function in patients with Parkinson′s disease and depression(DPD). Methods　Forty-eight patients with DPD(the DPD group) and 47 patients with Parkinson′s disease but without depression(the NDPD group) who were admitted to the Department of Neurology of Guangdong Second Provincial General Hospital from June 2021 to October 2022 were selected. Forty-three healthy spouses of the Parkinson′s disease(PD) patients were selected as the control group. Fecal samples of the research subjects were collected, and the bacterial deoxyribonucleic acid(DNA) in the samples was extracted for 16SrDNA sequencing. The structural differences of the gut microbiota were compared among the three groups, and the different bacterial genus in the DPD group were analyzed. PICRUSt2 software was used to analyze the metabolic function of the gut microbiota sequencing results. Results　Compared with that in the control group, the α diversities of microflora in the DPD group and the NDPD group were significantly increased(P<0.05). The results of β diversity analysis showed that the difference in the composition of gut microbiota between the DPD group and the NDPD group was statistically significant(P<0.05). Compared with that in the control group and the NDPD group, the abundance of Actinobacteria and Synergistetes in the DPD group was increased. There were significant differences in the increased abundances of Actinobacteria and Synergistetes between the DPD group and the control group(P<0.05), but there was no significant difference between the DPD group and the NDPD group(P>0.05). The results of linear discriminant analysis(LDA) showed that Alistipes, Anaerotruncus, Dielma, Holdemania, Cloacibacillus and Collinsella were the characteristic gut microbiota of the DPD patients. The results of PICRUSt2 metabolic function analysis showed that carbon fixation in photosynthetic organism and ascorbic acid resistance were the main metabolic pathways of the gut microbiota of the DPD patients. Conclusion　The gut microbiota of the DPD patients is significantly different from that of the NDPD patients and the healthy people. The characteristic gut microbiota of the DPD patients may participate in the pathogenesis of the diseases through the pathways of protein acylation and ascorbic acid resistance.]]> 2023/4/30 11:53:10 REN Teng-zhu, LI Gui-hua, LIANG Xiao-yu, et al. 371true ［Abstract］　Objective　To construct a pre-trained Swin Transformer model, and to analyze its efficacy in diagnosis of diabetic retinopathy. Methods　In the training dataset of the APTOS 2019 Blindness Detection Competition was downloaded from the Data Modeling and Data Analysis of Competition Platform(https://www.kaggle.com/competitions/aptos2019-blindness-detection). The OpenCV Image Processing Library was used to augment the data by changing the brightness, flipping different angles and equalizing the histogram, and a total of 9 160 color fundus images were obtained as a complete dataset. The pre-trained Swin Transformer model was constructed to classify the grades of pathological changes in the images, and its results were compared with the training results of the four pre-trained Vision Transformer, EfficientNetV2, ResNet-50 and GoogLeNet neural network models. In addition, the impact of pre-training on the model was compared with the non-pre-trained Swin Transformer model with random initialization parameters. Results　The quadratic weighted Kappa value of the pre-trained Swin Transformer model was 0.977, with an accuracy rate being 94.6%. Compared with the accuracy rates in the Vision Transformer, EfficientNetV2, ResNet-50 and GoogLeNet models, the accuracy rates in the pre-trained Swin Transformer model were increased by 1.9%, 2.3%, 5.4% and 7.1%, respectively. Compared with those in the non-pre-trained Swin Transformer model, the accuracy rate in the pre-trained Swin Transformer model was increased by 4.4%, and the training rounds were reduced by nearly 400 times. Conclusion　The pre-trained Swin Transformer model has high diagnostic accuracy for diabetic retinopathy and good clinical application value.]]> 2023/4/30 11:53:10 WANG Gang, WANG Hong-min, WANG Shan-zhi, et al. 370true ［Abstract］　Objective　To investigate the association of serum interleukin-33(IL-33) levels and expression levels of bone turnover markers with fracture risk in elderly women with post-menopausal osteoporosis(PMO). Methods　Two hundred and seventy-seven elderly female PMO patients with fragility fractures due to falls who were admitted to Chongming Hospital Affiliated to Shanghai University of Medicine and Health Sciences from June 2019 to March 2021 were recruited as the fracture group, and other 250 elderly female PMO patients with no fracture records during the same period were included as the control group. The serum IL-33 levels were compared between the two groups. Spearman rank correlation analysis was used to explore the correlation between serum IL-33 levels and the levels of bone turnover markers. Multivariate logistic regression was used to analyze the risk factors of fracture in the PMO women. Receiver operating characteristic(ROC) curve was used to analyze the efficacy of serum IL-33 levels in predicting fractures in the women with PMO. Results　The levels of serum parathyroid hormone(PTH), C-terminal telopeptide of type 1 collagen(CTX-1), procollagen type 1 N-terminal propeptide(P1NP), osteocalcin(OC) and bone-specific alkaline phosphatase(BALP) in the fracture group were higher than those in the control group, and the serum 25-hydroxyvitamin D［25(OH)D］ levels and bone mineral density(BMD) in the fracture group were lower than those in the control group, and the differences were statistically significant(P<0.05). The results of Spearman rank correlation analysis showed that the serum IL-33 level in the fracture group was positively correlated with the serum 25(OH)D level(r_s=0.158, P=0.009), and was negatively correlated with the serum levels of CTX-1(r_s=-0.233, P<0.001), OC(r_s=-0.357, P<0.001) and PTH(r_s=-0.326, P<0.001). The results of multivariate logistic regression analysis showed that higher levels of serum IL-33(OR=0.125) and BMD(OR=0.554) were the protective factors for inhibiting fractures in the PMO women(P<0.05). The results of ROC curve analysis showed that serum IL-33 level has the application value in predicting the risk of fracture in the PMO women(P<0.05). The optimal cut-off value was 6.57 pg/ml, and the corresponding sensitivity and specificity were 94.80% and 91.30%, respectively. Conclusion　Serum levels of IL-33 and the levels of bone turnover markers are associated with fracture risk in a female population with PMO, and monitoring their levels is helpful for clinicians to timely intervene in at-risk patients.]]> 2023/4/30 11:53:10 YIN Le, ZHANG Hao-jie, ZHANG Ping-chao, et al. 369true ［Abstract］　Objective　To analyze the correlation between serum cystatin C(Cys C) level and futile recanalization in patients with anterior circulation large vessel occlusion after mechanical thrombectomy. Methods　One hundred and ten acute ischemic stroke patients with anterior circulation large vessel occlusion who underwent mechanical thrombectomy in the Affiliated Hospital of North China University of Science and Technology from February 2019 to May 2022 were selected, and according to their Modified Rankin Scale(mRS) scores 3 months after operation, the patients were divided into the effective recanalization group(with mRS scores <3 points, 49 cases) and the futile recanalization group(with mRS scores ≥3 points, 61 cases). The general clinical data were compared between the patients in the two groups. Multivariate logistic regression was used to analyze the risk factors of futile recanalization after mechanical thrombectomy in the patients with anterior circulation large vessel occlusion. Receiver operating characteristic(ROC) curve was used to evaluate the predictive value of Cys C for futile recanalization. Results　Higher preoperative serum Cys C level(OR=1.007) and National Institutes of Health Stroke Scale(NIHSS)scores(OR=1.936) were the independent risk factors for futile recanalization in acute ischemic stroke patients with anterior circulation large vessel occlusion after mechanical thrombectomy(P<0.05), and both of the two indicators had the application value of predicting futile recanalization(AUC=0.922, AUC=0.884, P<0.05), and combining the two indicators could further improve the predicting efficiency(AUC=0.962; P<0.05). Conclusion　Serum Cys C level and NIHSS score are associated with futile recanalization after mechanical thrombectomy in patients with anterior circulation large vessel occlusion, which is helpful for clinicians to evaluate the prognosis of the patients and identify the patients with advantages of mechanical thrombectomy.]]> 2023/4/30 11:53:10 LI Guan-yi, ZHANG Chun-yang, LI Hong, et al. 368true ［Abstract］　Objective　To analyse the clinical efficacy of minimally invasive cardiac surgery under total thoracoscopy. Methods　A retrospective analysis was performed on 206 patients who underwent minimally invasive cardiac surgery under total thoracoscopy in the Department of Thoracic and Cardiovascular Surgery of the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2013 to May 2021, including 109 cases of atrial septal defect among whom 32 cases were complicated with moderate to severe tricuspid regurgitation, 58 cases of mitral valve disease among whom 28 cases were complicated with moderate to severe tricuspid regurgitation and 6 cases were complicated with thrombus, 22 cases of simple tricuspid regurgitation and 17 cases of left atrial myxoma. Results　All the operations were performed successfully. One case of enlarged incision was changed to thoracoscopic assisted small incision but there was no secondary thoracotomy for hemostasis. There was no perioperative mortality. There was no residual leakage or perivalvular leakage. The operation time was (268.8±70.6)min. The duration of extracorporeal circulation was (140.3±64.7)min. The duration of ascending aorta occlusion was (98.1±56.3)min. The intraoperative blood loss was (230±140)ml. The amount of plasma used during operation was (232±245)ml. The amount of red blood cells used during operation was (2.1±1.5 )U. The length of stay in the Intensive Care Unit(ICU) was (28.6±22.4)h. The auxiliary time of ventilator was (16.4±10.3)h. The postoperative drainage volume was (598±210)ml. The postoperative plasma transfusion was (446±330)ml. The average time to remove the drainage tube after operation was 4.8 days. The postoperative hospital stay was (12.6±4.7)days. Fifty-six point eight percent(117/206) of the patients did not receive blood transfusion during hospitalization. All the patients were followed up for 1 to 60 months after operation, and the cardiac function of the patients was good, and their New York Heart Association(NYHA) grades were Ⅰ to Ⅱ. Conclusion　Minimally invasive cardiac surgery under total thoracoscopy has the advantages of small damage, easy to accept and quick recovery, and should be recommended for a wider use in clinical practice.]]> 2023/4/30 11:53:10 ZHANG Ze, JIANG Wei, LI Si-cong, et al. 367true ［Abstract］　Objective　To investigate the anatomical features of internal carotid siphon in patients with intracranial aneurysms and their associations with aneurysm ruptures. Methods　A retrospective analysis was performed on 172 patients with intracranial aneurysms who were admitted to Tianjin Huanhu Hospital from October 2019 to November 2021, and theywere divided into a ruptured group(67 cases) and an unruptured group(105 cases) according to their aneurysm ruptures. The general clinical data, the morphology and location of aneurysms, as well as the measurement indicators of aneurysms and the measurement indicators of the internal carotid siphon on the side of intracranial aneurysms were compared between the two groups. Results　There were no significant differences in age, gender, history of hypertension, diabetes, smoking and alcohol drinking between the two groups(P>0.05). The proportion of irregular aneurysm morphology in the ruptured group was significantly higher than that in the unruptured group(47.76% vs 8.57%, P<0.05). The anterior bend angle and the anterosuperior bend angle of the internal carotid siphon in the ruptured group were larger than those in the unruptured group, and the differences were statistically significant(P<0.05). The results of binary logistic regression analysis showed that irregular aneurysm morphology(OR=5.722) and larger anterior bend angle of internal carotid siphon(OR=1.195) were the risk factors to promote aneurysm ruptures(P<0.05). The results of receiver operating characteristic(ROC) curve analysis showed that the likelihood of intracranial aneurysms rupturing increased significantly if the anterior bend angle of the internal carotid siphon was larger than 29.38°. Conclusion　The intracranial irregular aneurysm morphology and the increase of anterior bend angle of internal carotid siphon are independent risk factors for intracranial aneurysm ruptures. Clinicians should pay attention to the screening of high-risk patients and give them timely intervention.]]> 2023/4/30 11:53:10 YANG Xiao, GUO Jing-jing, LIU Song, et al. 366true ［Abstract］　Objective　To investigate the effects of normal range of CD4⁺ T cell levels on insulin resistance in patients with type 2 diabetes mellitus(T2DM). Methods　Two hundred and sixty-nine patients with T2DM who were admitted to the Affiliated Hospital of Xuzhou Medical University from September 2021 to June 2022 were recruited and according to their normal range of CD4⁺ T cell levels the patients were divided into 4 groups: group A(58 cases, 414-670 cells /μl), group B(78 cases, 671-927 cells /μl), group C(74 cases, 928-1 183 cells /μl) and group D(59 cases, 1 184-1 440 cells /μl). The indicators of the subjects including interferon-γ(INF-γ), interleukin-17(IL-17), total cholesterol(TC), triglyceride(TG) and C-reactive protein(CRP) were detected. Oral glucose tolerance test(OGTT) and insulin release test were performed on the patients. Homeostasis Model Assessment-β(HOMA-β), Homeostasis Model Assessment-Insulin Resistance Index(HOMA-IR) and Matsuda Insulin Sensitivity Index(ISI_M) were calculated, and the correlation among all the indicators was analyzed. Results　There were statistically significant differences in CRP, INF-γ, IL-17, glycosylated hemoglobin(HbA_1c), fasting plasma glucose(FPG), HOMA-IR and ISI_M among the four groups(P<0.05). CRP, INF-γ, IL-17, HbA_1c, FPG and HOMA-IR were positively correlated with CD4⁺ T cell levels, while ISI_M was negatively correlated with CD4⁺ T cell levels. The results of Spearman rank correlation analysis showed that HOMA-IR was significantly positively correlated with CD4⁺ T cells, CRP, HbA_1c, FBG, IL-17 and INF-γ(P<0.05), and was significantly negatively correlated with ISI_M(P<0.05). The results of multiple linear regression analysis showed that CD4⁺ T cells, FBG, IL-17, INF-γ levels were positively correlated with HOMA-IR levels(P<0.05). After controlling FPG and the other confounding factors(CRP, HbA_1c, IL-17 and INF-γ), the results of partial correlation analysis showed that HOMA-IR was positively correlated with CD4⁺ T cells(r=0.343, P<0.05). Conclusion　Within the normal range of CD4⁺ T cell levels, the higher the level of CD4⁺ T cells in patients with T2DM, the more severe the insulin resistance and the lower the sensitivity of peripheral tissues to insulin.]]> 2023/4/30 11:53:10 ZHANG Zhen-hua, SUN Juan, HE Miao-miao, et al. 365true ［Abstract］　Objective　To investigate the correlation of salivary cortisol level and periodontal status with coronary atherosclerotic heart disease(CHD). Methods　Eighty-nine patients admitted to Chaohu Hospital of Anhui Medical University from December 2021 to February 2022 due to chest pain and chest tightness were selected and divided into CHD group(44 cases) and non-CHD group(45 cases) according to the results of coronary angiography. The periodontal conditions including calculus index(CI), exploratory bleeding index(BI), exploratory periodontal depth(PD) and attachment loss(AL) were examined in the two groups. The Oral Health Impact Profile-14(OHIP-14) scores and salivary cortisol levels were compared between the two groups. Results　The PD and AL levels and the OHIP-14 scores in the CHD group were higher than those in the non-CHD group, and the differences were statistically significant(P<0.05). The salivary cortisol level in the CHD group was significantly higher than that in the non-CHD group［(9.75±1.63)nmol/L vs (7.41±1.22)nmol/L; t=8.286, P<0.001］. The results of binary logistic regression analysis showed that higher level of salivary cortisol, and higher scores of AL and OHIP-14 were the risk factors for CHD(P<0.05). The results of receiver operating characteristic(ROC) curve analysis showed that salivary cortisol had the application value in diagnosis of CHD［area under the curve(AUC)(95%CI)= 0.893(0.830-0.956), P<0.001］. Conclusion　The level of salivary cortisol and periodontal health status are correlated with the occurrence and development of CHD, and the higher level of salivary cortisol is a risk factor for promoting the occurrence of CHD.]]> 2023/4/30 11:53:10 CHENG Yu, DING Hong-zhong 364true ［Abstract］　Objective　To investigate the expression levels of long non-coding RNA taurine up-regulated gene 1(lncRNA TUG1) and microRNA-26a(miR-26a) in placental tissues of the patients with preeclampsia(PE), and to analyze their roles in the occurrence and development of PE. Methods　One hundred newly diagnosed PE patients(the PE group) admitted to the Department of Obstetrics of Zhuzhou Municipal Central Hospital from May 2019 to May 2021 were selected, including 49 patients with mild PE(the mild PE group) and 51 patients with severe PE(the severe PE group). One hundred healthy pregnant women were included in the control group during the same period based on the matching age and gestational age. The expression levels of lncRNA TUG1 and miR-26a in the placental tissues of the study subjects were detected by quantitative real-time polymerase chain reaction(qRT-PCR). Pearson correlation analysis was used to investigate the correlation between the expression levels of lncRNA TUG1 and miR-26a and the clinical indicators of the PE patients. Results　The expression levels of lncRNA TUG1 in the placental tissues in the mild PE group and the severe PE group were lower than those in the control group, and the expression levels of miR-26a in the mild PE group and the severe PE group were higher than those in the control group, and the differences were statistically significant(P<0.05). Compared with those in the placental tissues of the mild PE group, the expression level of lncRNA TUG1 was lower and the expression level of miR-26a was higher in the placental tissues of the severe PE group, and the differences were statistically significant(P<0.05). The results of Pearson correlation analysis showed that the expression level of lncRNA TUG1 in the placental tissues of the PE patients was negatively correlated with systolic blood pressure, diastolic blood pressure and urinary protein level(P<0.05), and that the expression level of miR-26a was positively correlated with systolic blood pressure, diastolic blood pressure and urinary protein level(P<0.05). The expression level of lncRNA TUG1 was negatively correlated with that of miR-26a(P<0.05). Conclusion　lncRNA TUG1 is low expressed in PE placental tissues, while miR-26a is highly expressed, which is associated with the severity of PE. lncRNA TUG1 may participate in the occurrence and development of PE by targeting miR-26a.]]> 2023/4/30 11:53:10 ZHANG Chan, ZHANG Jing 363true ［Abstract］　Objective　To investigate the effect of low expression of γ3 subunit of laminins(LAMC3) on paclitaxel(PTX) resistance in ovarian cancer(OC) cells. Methods　Western blot was used to detect the expression of LAMC3 in the PTX-resistant OC cells HeyA8-R(H-R) and its parent cells HeyA8(H). Stable transfected cell line H-R-C3 shRNA3 with low expression of LAMC3 and its control cell line H-R-C3 Scramble was constructed by transfection of lentivirus into H-R cells. CCK-8 assay was used to detect the half maximal inhibitory concentration of cells to PTX(IC₅₀). The cell clone formation ability was measured by plate clone formation assay. Western blot was also used to detect the expression levels of key pathway proteins such as multidrug resistance, ferroptosis, cell cycle and apoptosis. Results　LAMC3 was significantly down-regulated in the PTX-resistant OC cells H-R and H-R-C3 shRNA3 compared with its parent cells H and control cells H-R-C3 Scramble. The knockdown of the expression of LAMC3 not only reduced the resistance to PTX but also decreased the clone formation ability of PTX-resistant OC cells. The results of Western blot showed that compared with those in the H-R-C3 Scramble cells, the protein expression levels of multidrug resistance 1/ATP-binding cassette subfamily B member 1(MDR1/ABCB1) decreased, and the solute carrier family 7 member 11(SLC7A11) increased, and the cyclin p21 Waf1/Cip1 and cyclin E2(CCNE2) increased, and the cyclin dependent kinase 2(CDK2) and cyclin dependent kinase 4(CDK4) decreased in the H-R-C3 shRNA3 cells under the concentration gradient treatment of PTX. Moreover, cleaved caspase-9, cleaved caspase-3, cleaved poly ADP-ribose polymerase(cleaved PARP) increased which belonged to subunit proteins when produced during apoptosis. All the differences were statistically significant(P<0.05). Conclusion　Low expression of LAMC3 may reduce the resistance of PTX-resistant OC cells to PTX by affecting multidrug resistance, cell cycle and apoptosis, suggesting that LAMC3 may become a new potential target for drug resistance treatment in OC.]]> 2023/3/30 12:08:59 XIA Lian-ping, YIN Fu-qiang 362true ［Abstract］　Objective　To investigate the relationship between plasma fibrinogen level and enlarged perivascular spaces(EPVS) in patients with acute lacunar cerebral infarction. Methods　The clinical data of 172 acute lacunar cerebral infarction patients with EPVS who were admitted to the Second People′s Hospital of Lianyungang City from January 2021 to June 2022 were retrospectively analyzed. The number and severity of EPVS were evaluated by head magnetic resonance imaging(MRI), which were divided into basal ganglia EPVS(BG-EPVS) and centrum semiovale EPVS(CSO-EPVS). The patients were divided into the mild group(grade 0-1:≤10 EPVS) and the moderate to severe group(grade 2-4: >10 EPVS). The general clinical data were compared between the two groups, and multivariate logistic regression was used to analyze the relationship between plasma fibrinogen level and EPVS progression. Receiver operating characteristic(ROC) curve was used to analyze the efficacy of plasma fibrinogen level in differentiating mild from moderate to severe EPVS. Results　Among the 172 patients, 57 cases(33.14%) were classified as mild BG-EPVS, and 115 cases(66.86%) were classified as moderate to severe BG-EPVS. There were 100 patients(58.14%) with mild CSO-EPVS and 72 patients(41.86%) with moderate to severe CSO-EPVS. Compared with the mild BG-EPVS group, the moderate to severe BG-EPVS group was older, and had more patients with hypertension, and had higher levels of total cholesterol(TC), high-density lipoprotein(HDL) and fibrinogen, with statistically significant differences between the two groups(P<0.05). The age of the moderate to severe CSO-EPVS group was significantly older than that of the mild CSO-EPVS group(P<0.05), but there were no statistically significant differences in the other indicators between the two groups(P>0.05). The results of multivariate logistic regression analysis showed that older age(OR=1.043), complicated with hypertension(OR=2.443), and higher fibrinogen level(OR=2.227) were independent risk factors for the progression of BG-EPVS(P<0.05). The results of ROC curve analysis showed that plasma fibrinogen level could effectively distinguish mild BG-EPVS from moderate to severe BG-EPVS［AUC(95%CI)=0.693(0.611～0.775), P<0.001］. The optimal cut-off value was 4.00 g/L, and the corresponding sensitivity and specificity were 61.70% and 80.70%, respectively. Conclusion　Plasma fibrinogen level is associated with the progression of BG-EPVS in patients with acute lacunar cerebral infarction, and can be used in the differential diagnosis of mild and moderate to severe BG-EPVS.]]> 2023/3/30 12:08:59 SHAO Yun-yun, HE Qian-qian, ZHENG Xue, et al. 361true ［Abstract］　Objective　To analyze the association of serum polyclonal combined free light chain(cFLC) with early improvement of cardiac function and prognosis after percutaneous coronary intervention(PCI) in patients with ST-segment elevation myocardial infarction(STEMI). Methods　Three hundred and thirty-two STEMI patients(the STEMI group) who underwent PCI in Inner Mongolia Autonomous Region People′s Hospital from January 2019 to December 2021 were selected, and 162 patients without coronary heart disease during the same period were selected as the control group. N-Latex FLC system and scattering turbidimetric method were used to determine the serum cFLC levels at admission, 72 hours, 7 days and 30 days after onset. A follow-up was performed on the patients after PCI. Echocardiography was conducted 24 hours and 3 months after admission, and major adverse cardiovascular events(MACEs) were recorded. Results　The serum cFLC level in the STEMI group peaked 7 days after onset, which was higher than that in the control group at admission(P<0.05). The patients in the STEMI group were divided into the low cFLC group(<0.66 mg/dl, n=166) and the high cFLC group(≥0.66 mg/dl, n=166), using the median value of serum cFLC as the threshold 7 days after onset. The peak cardiac troponin I(cTnI), peak N-terminal pro-brain natriuretic peptide(NT-proBNP), peak high-sensitive C-reactive protein(hs-CRP), SYNTAX score, left ventricular end-diastolic diameter(LVEDD), left ventricular end-systolic diameter(LVESD), left ventricular end-diastolic volume(LVEDV) and left ventricular end-systolic volume(LVESV) in the high cFLC group were higher than those in the low cFLC group at admission, and the differences were statistically significant(P<0.05). After PCI, the left ventricular ejection fraction(LVEF) in the low cFLC group was significantly improved compared with that before PCI(Z=3.578, P<0.001), but the LVEF in the high cFLC group was not significantly improved compared with that before PCI(Z=0.612, P=0.544). The results of multivariate Cox regression analysis showed that higher serum cFLC level 7 days after onset was an independent risk factor for MACEs after PCI in the STEMI patients(P<0.05), and the higher serum cFLC level 7 days after onset had application value in predicting the occurrence of MACEs ［AUC(95%CI)=0.924(0.889～0.960)］. Conclusion　Early elevation of serum cFLC in acute STEMI patients is associated with poor improvement of left ventricular function after PCI and the occurrence of MACEs, which has a promising application as a novel biomarker for left ventricular dysfunction.]]> 2023/3/30 12:08:59 MENG Jun-jun, WU Zhen-li, SONG Ming-zhe, et al. 360true ［Abstract］　Objective　To observe the therapeutic effect of microwave combined with sodium aescinate on herpes zoster in middle and elderly patients. Methods　One hundred and twenty patients with herpes zoster admitted to the 63750th Military Hospital of the People′s Liberation Army of China from September 2018 to March 2022 were selected and divided into observation group and control group by random number table method, with 60 cases in each group. The control group received antiviral and nutritional neurotherapy, and the observation group received microwave treatment combined with sodium aescinate on the basis of the treatment plan of the control group. Dermatology Life Quality Index(DLQI) score, Numerical Rating Scale(NRS) score and Verbal Rating Scale(VRS) score were performed on both groups before treatment, and 1 week and 2 weeks after treatment. The pain relief time, herpes scab time, skin fading time and the incidence of post-herpetic neuralgia(PHN) were compared between the two groups. Results　After treatment, the scores of DLQI, NRS and VRS in both groups decreased compared with those before treatment, and the scores of the observation group decreased more significantly(P<0.05). The DLQI, NRS and VRS scores of the observation group were lower than those of the control group 1 week and 2 weeks after treatment, and the differences were statistically significant(P<0.05). The pain relief time, herpes scab time and skin fading time in the observation group were shorter than those in the control group, and the differences were statistically significant(P<0.05). Six cases(10.00%) in the control group developed PHN, and 2 cases(3.33%) in the observation group developed PHN 4 weeks after the skin lesions subsided, and there was no significant difference in the incidence of PHN between the two groups(χ²=1.205, P=0.272). There was no significant difference in the incidence of adverse reactions between the two groups(5.00% vs 3.33%; χ²=0.000, P=1.000). Conclusion　Microwave combined with sodium aescinate treatment can effectively relieve the pain symptoms of herpes zoster, promote the healing of herpes scab and skin lesions, and improve the quality of life of the patients.]]> 2023/3/30 12:08:59 LI Xiao-bin, QUAN Dong, LIU Xuan, et al. 359true ［Abstract］　Objective　To analyze the efficacy of modified age, blood pressure, clinical features, duration, and diabetes(ABCD2) score combined with the detections of serum adenosine deaminase(ADA) and low-density lipoprotein cholesterol levels(LDL-C) in etiological diagnosis of vertigo. Methods　Two hundred and fifty-nine vertigo patients admitted to the Department of Neurology of the Second People′s Hospital of Shanxi Province from September 2019 to September 2022 were selected, including 129 cases of posterior circulation stroke(PCS) patients with isolated vertigo(the PCS group) and 130 patients with peripheral vestibular vertigo(the control group). All the patients were evaluated by modified ABCD2 score, and the serum levels of ADA, LDL-C and other biochemical indicators were detected. Receiver operating characteristic(ROC) curve method was used to analyze the efficacy of modified ABCD2 score combined with the detections of serum ADA and LDL-C levels in etiological diagnosis of vertigo. Results　The patients in the PCS group were older than those in the control group, and the proportion of the patients complicated with hypertension, diabetes and hyperlipidemia in the PCS group was higher than that in the control group, and the levels of serum ADA, total cholesterol(TC), LDL-C, fasting plasma glucose(FPG), white blood cell count and the modified ABCD2 score were higher than those in the control group, and the level of HDL-C in the PCS group was lower than that in the control group, with statistically significant differences between the two groups(P<0.05). The results of ultivariate logistic regression analysis showed that higher modified ABCD2 scores, higher levels of LDL-C, ADA, and concomitant diabetes were the independent risk factors of PCS in the patients with isolated vertigo onset(P<0.05). The results of ROC curve analysis showed that the modified ABCD2 scores, LDL-C and ADA all had the application value in the diagnosis of PCS in the patients with isolated vertigo onset(P<0.05), among which the modified ABCD2 scores had the best diagnostic efficacy［area under the curve(AUC)=0.936］. The combination of the three indicators was performed for further analysis, and the results showed that the diagnostic efficacy of the combination of the three indicators was higher than that of any of the three indicators(AUC=0.990). Conclusion　The modified ABCD2 scores and serum ADA and LDL-C levels of the PCS patients with isolated vertigo are significantly higher than those of the patients with peripheral vestibular vertigo, which is helpful to identify the cause of vertigo.]]> 2023/3/30 12:08:59 REN Yun, XING Chao-xia 358true ［Abstract］　Objective　To analyze the correlation between neutrophil-to-high density lipoprotein cholesterol ratio(NHR) and the severity of coronary artery stenosis in patients with acute coronary syndrome(ACS). Methods　The clinical data of 270 ACS patients admitted to the Department of Cardiology of Subei People′s Hospital from May 2021 to May 2022 were retrospectively collected, and coronary artery angiography(CAG) was performed on the patients. According to different Gensini scores, the patients were divided into low GS group(Gensini scores ≤48 points, 130 cases) and high GS group(Gensini scores>48 points, 140 cases). Binary logistic regression was used to analyze the independent influencing factors of ACS patients with high Gensini scores. Receiver operating characteristic(ROC) curve was used to analyze the differential diagnostic efficacy of NHR in the ACS patients with high and low Gensini scores. Results　The proportion of the patients complicated with diabetes in the high GS group was higher than that in the low GS group. The levels of neutrophils(NEU), platelets(PLT), monocytes(MON) and NHR in the high GS group were higher than those in the low GS group, and the levels of lymphocytes(LYM) and serum albumin(ALB) in the high GS group were lower than those in the low GS group, and the differences were statistically significant(P<0.05). The results of binary logistic regression analysis showed that concomitant diabetes(OR=1.873) and high level of NHR(OR=1.430) were the independent influencing factors promoting the occurrence of ACS in the patients with high Gensini scores(P<0.05), while high levels of LYM(OR=0.585) and ALB(OR=0.931) were the independent influencing factors inhibiting the occurrence of ACS in the patients with high Gensini scores(P<0.05). The results of ROC curve analysis showed that NHR had the application value of differentiating the ACS patients with high Gensini scores from the ACS patients with low Gensini scores［AUC(95%CI)=0.710(0.649-0.770), P<0.001］, and the optimum cut-off value was 7.40, and the corresponding specificity and sensitivity were 83.1% and 51.4%. Conclusion　NHR is positively correlated with the degree of coronary artery stenosis, and has a certain diagnostic value for the severity of coronary artery in ACS patients.]]> 2023/3/30 12:08:59 HUANG Jing, XU Ri-xin, WANG Da-xin 357true ［Abstract］　Objective　To evaluate the safety and effectiveness of left bundle branch pacing(LBBP) in treatment of severe atrioventricular block. Methods　Sixty inpatients with type Ⅱ second-degree atrioventricular block or third-degree atrioventricular block who were treated with permanent pacemaker implantation in the People′s Hospital of Guangxi Zhuang Autonomous Region from March 2019 to January 2020 were selected. The inpatients were divided into LBBP group and right ventricular outflow tract pacing(RVOTP) group by random number table method, with 30 cases in each group. During operation, and 1 week, 1 month, 3 months and 6 months after operation, the ventricular electrode capture threshold, electrode impedance, and R wave amplitude of the two groups were monitored, and the QRS wave width was measured by electrocardiogram. Cardiac color Doppler ultrasound was adopted to detect left ventricular end-diastolic diameter(LVEDd) and left ventricular ejection fraction(LVEF). Chest X-ray was performed to check whether there was electrode dislocation and rountine follow-up was performed to check whether there was pacemaker pocket infection was observed. Results　During the follow-up process, the ventricular electrode capture thresholds showed a downward trend in the two groups, and the levels of capture threshold in the LBBP group were significantly lower than those in the RVOTP group at each time point(P<0.05). The R wave amplitude showed an upward trend, and the electrode impedance showed a downward trend in the two groups, but there were no statistically significant differences in the R wave amplitude and the electrode impedance between two groups(P>0.05). At each time point of follow-up, the QRS width of the LBBP group was significantly narrower than that of the RVOTP group(P<0.05), but there were no significant differences between the two groups in LVEDd and LVEF indicators(P>0.05). There were no pacemaker pocket infection and pacemaker electrode dislocation in the two groups. In the LBBP group, there were 1 case of ventricular septal perforation and 1 case of septal vascular injury. There were no significant differences in the incidence rates of intraoperative and postoperative complications between the two groups(P>0.05). Conclusion　LBBP is safe and effective in the treatment of atrioventricular block, and it is worthy of clinical promotion.]]> 2023/3/30 12:08:59 LU Zheng-de, SHI Lei, SHI Ying, et al. 356true ［Abstract］　Objective　To investigate the application effect of Rh antigen typing and irregular blood group antibody screening in precise blood transfusion. Methods　Two hundred patients who were hospitalized in Qinglong Manchu Autonomous County Hospital and needed clinical blood transfusion from January 1, 2019 to September 30, 2021 were selected, and the age of the patients ranged from 12 to 71(48.43 ± 1.45)years. One hundred blood transfusion cases without five Rh(D, C, c, E, and e) blood group antigens test from January 1, 2019 to August 31, 2020 were selected as the control group, and one hundred blood transfusion cases who underwent compatible blood matching by Rh blood group antigen detection from September 1, 2020 to September 30, 2021 were selected as the experimental group. Before the blood products were put into the warehouse, the anti-human globulin cassette method was used to test the ABO blood groups and the five Rh(D, C, c, E, and e) blood group antigens and irregular antibodies, and the results were input into the Blood Transfusion Information Management System. When in use, the Blood Transfusion Information Management System was used to automatically match blood bags with the ABO blood groups and the five Rh(D, C, c, E, and e) blood group antigens for the recipients in the experimental group. The saline method and the anti-human globulin cassette method were used for conducting cross-matching test and the cross-matching test was carried out to achieve clinical precise blood transfusion. Results　Irregular antibodies were detected in 11 cases(11.00%) in the control group, and 2 cases(2.00%) in the experimental group, and the difference was statistically significant(χ²=6.664, P=0.010). There were no cases of adverse blood transfusion reaction in the experimental group. In the control group, 1 case of adverse blood transfusion reaction occurred, which was caused by the production of the patient′s irregular blood group anti-E antibody. After difficult blood matching and infusion of 4 U of compatible blood, the patient′s hemoglobin concentration rose to the expected level, and the patient recovered and was discharged from the hospital. Compared with those in the control group, the difficult blood matching time and hospital stay were shorter, and the amount of transfused leukocyte-free suspended red blood cells was less in the experimental group, and the differences were statistically significant(P<0.05). Conclusion　The detection of Rh blood group antigens is helpful to achieve clinical precise blood transfusion, and effectively avoids the production of irregular antibodies, and reduces the occurrence of clinical blood transfusion adverse reactions, and improves the safety of clinical blood transfusion treatment, which is worthy of clinical promotion.]]> 2023/3/30 12:08:59 LI Xiu-ling, ZHANG Dong-min, KONG Qing-he, et al. 355true ［Abstract］　Objective　To observe the effect of minimally invasive puncture combined with middle meningeal artery embolization(MMAE) on treatment of chronic subdural hematoma(CSDH). Methods　The clinical data of 52 patients with unilateral CSDH who were admitted to Affiliated Hospital of Yangzhou University from June 2019 to June 2021 were selected and the patients were divided into the observation group(receiving minimally invasive puncture combined with MMAE treatment, 24 cases) and the control group(receiving minimally invasive puncture treatment, 28 cases) according to different surgical methods. The absorption of residual hematoma, reoperation rate and Markwalder′s neurological function grade were compared between the two groups 3 months after surgery. Results　The incidence of residual hematoma 3 months after surgery in the observation group was lower than that in the control group, and the improvement of Markwalder′s neurological function grade in the observation group was better than that in the control group, with statistically significant differences between the two gropus(P<0.05). There were no reoperation cases in the observation group, and 4 cases in the control group had hematoma recurrence and aggravated clinical symptoms, requiring minimally invasive puncture surgery again, and the postoperative effect was good. There was no statistically significant difference in the rate of reoperation between the two groups(P>0.05). Conclusion　Minimally invasive puncture combined with MMAE can effectively promote the absorption of residual hematoma in the treatment for CSDH patients, reduce the risk of reoperation, and help to improve the neurological function of the patients, which is worthy of clinical recommendation.]]> 2023/3/30 12:08:59 CAO De-mao, DUAN Xiao-chun, LIU Zhen-sheng, et al. 354true ［Abstract］　Objective　To construct sorafenib-resistant hepatocellular carcinoma cell line and to explore its drug-resistant mechanism. Methods　HepG2 and Huh7 cells were used to establish sorafenib-resistant cell models by stepwise increase in drug concentrations method. The sensitivity of cells to sorafenib at different concentrations was detected by CCK8 assay. The migration ability of cells was detected by wound healing assay. The apoptosis of cells was analyzed by flow cytometry. The expression levels of apoptosis-related factor caspase-3 and autophagy marker factors P62 and LC3 were detected by real-time quantitative reverse transcription-polymerase chain reaction(RT-PCR) and Western blot. Results　Sorafenib-resistant hepatocellular carcinoma cell lines HepG2-SR and Huh7-SR were obtained.With the increase of sorafenib intervention concentration, the survival rates of drug-resistant cell lines and wild-type cell lines decreased gradually. The half inhibitory concentration(IC₅₀) of HepG2-SR cells was significantly higher than that of HepG2 cells［(15.74±0.38)μM vs (7.27±0.44)μM; t=5.450, P<0.001］. The IC₅₀ of Huh7-SR cells was significantly higher than that of Huh7 cells［(11.73±0.27)μM vs (4.92±0.31)μM; t=4.807, P<0.001］. Compared with the wild-type cell lines, the drug-resistant cell lines showed higher anti-apoptosis and migration ability. The expression levels of caspase-3 and P62 in the drug-resistant cell lines were significantly lower than those in the wild-type cell lines(P<0.05), while the expression level of LC3 in the drug-resistant cell lines was significantly higher than that in the wild-type cell lines(P<0.05). Conclusion　Sorafenib-resistant hepatocellular carcinoma cell lines HepG2-SR and Huh7-SR have stronger anti-apoptosis, anti-reproductive inhibition and migration ability, and higher autophagy level, which provides a basis for studying the mechanism of sorafenib resistance in hepatocellular carcinoma.]]> 2023/2/28 12:21:57 ZENG Qiong-rong, LIU Ze-feng, WANG Wen-juan, et al. 353true ［Abstract］　Objective　To explore the changes and effects of vitamin D levels in children with simple obesity after comprehensive weight loss intervention. Methods　The children were selected as the research subjects from a primary school in Wuhan during October 2020 and March 2021, aged from 7 to 14 years, among whom 43 cases were simple obese children(the obese group) and 50 cases were normal weight(Wt) children(the control group). In the obese group, comprehensive weight loss intervention was carried out for 3 months, including diet, exercise guidance and health education. The levels of serum 25-hydroxyvitamin D［25-(OH)D］, anthropological and metabolic indicators in the normal group and the obese group were detected before and after intervention. Results　Compared with the control group, the obese group had higher Wt, waist circumference(WC), body mass index(BMI), BMI-Z, percentage body fat(PBF), fasting insulin(FINS), homeostasis model assessment of insulin resistance index(HOMA-IR) and triglyceride(TG), and lower fasting blood glucose(FBG) and high-density lipoprotein cholesterol(HDL-C) level before intervention, and the differences were statistically significant(P<0.05). Compared with those before intervention, WC decreased, and the levels of BMI-Z and 25-(OH)D decreased, and the total cholesterol(TC) level increased after intervention, and the differences were statistically significant(P<0.05). The results of Pearson correlation analysis showed that 25-(OH)D was negatively correlated with Wt, WC, BMI, BMI-Z, FINS and HOMA-IR(P<0.05), and positively correlated with HDL-C(P<0.05). The results of multiple linear regression analysis showed that △25-(OH)D was negatively correlated with △WC(P<0.01) and △FBG(P<0.05), that is, the greater the decrease of 25-(OH)D, the smaller the decrease of WC and FBG. Conclusion　Vitamin D is closely related to the level of metabolic indexes in children with simple obesity. The decrease of vitamin D level affects the effect of weight loss in children with simple obesity.]]> 2023/2/28 12:21:57 LI Shi-han, LI Rui-zhen, HE Pei-ling, et al. 352true ［Abstract］　Objective　To observe the effects of different ventilation strategies on mechanical power(MP) and inflammatory factor levels in patients undergoing laparoscopic colorectal cancer surgery. Methods　Sixty patients who underwent laparoscopic colorectal cancer surgery in Guangzhou Red Cross Hospital from May 2021 to November 2021 were selected and divided into conventional ventilation group(group V) and lung protective ventilation group(group P) by random number table method, with 30 cases in each group. Group V: tidal volume(VT)=9 ml/kg, positive end expiratory pressure(PEEP)=0 cmH₂O; Group P: VT=7 ml/kg, PEEP=5 cmH₂O. Peak airway pressure(P_peak), airway plateau pressure(P_plat) and pulmonary dynamic compliance(Cdyn) were recorded 5 minutes after endotracheal intubation(T₁), 10 minutes(T₂) and 60 minutes(T₃) after establishment of CO₂ pneumoperitoneum, and 10 minutes after pneumoperitoneum disappeared(T₄), and MP was calculated. Radial artery blood were collected for blood gas analysis at T₁, T₂, T₃ and when the patients entered the postanesthesia care unit(PACU), and pH, arterial partial pressure of carbon dioxide(PaCO₂) and arterial partial pressure of oxygen(PaO₂) were recorded, and oxygenation index(OI) and alveolar-arterial oxygen differential(P_A-aO₂) were calculated. The levels of lung Clara cell secretory protein(CC-16), interleukin-6(IL-6) and neutrophil elastase(NE) in serum were detected at T₁, T₃ and immediately after operation, respectively. Results　MP showed an increasing trend in both groups at the time points from T₂ to T₄, and the serum levels of CC-16, IL-6 and NE were significantly increased at T₃ and after surgery compared with those at T₁(P<0.05). At the time points of T₂ and T₃, the MP level in group P was lower than that in group V, and the difference was statistically significant(P<0.05). The serum levels of CC-16 and IL-6 in group P were significantly lower than those in group V immediately after operation(P<0.05). The total MP size was positively correlated with the changes of the serum CC-16, IL-6 and NE levels during pneumoperitoneum(P<0.05). Conclusion　The protective mechanism of lung protective ventilation strategy may be related to lower MP.]]> 2023/2/28 12:21:57 WANG Zuo-huan, WANG Lin, CAO Yang 351true ［Abstract］　Objective　To investigate the effect of prophylactic levofloxacin on reducing the risk of infection during CAT treatment in children with acute lymphoblastic leukemia(ALL). Methods　The clinical data of 324 children who were newly diagnosed with ALL and were admitted to the Second Affiliated Hospital of Anhui Medical University from January 2015 to August 2022 were retrospectively analyzed. A total of 264 children who did not take levofloxacin during induction therapy were taken as the control group, and 60 children who received prophylactic levofloxacin were taken as the observation group. The incidence rates of bloodstream infection(BSI), febrile neutropenia(FN), clinically documented infection(CDI), inflammatory indicators and use of antibiotics during CAT treatment were compared between the two groups. Results　The incidence rates of BSI, FN, serious infections, fever and CDI in the observation group were lower than those in the control group. The proportion of the patients using the number of antibiotics ≥3, and the proportion of the patients using cephalosporins and macrolides in the observation group were lower than those in the control group, and the level of procalcitonin(PCT) in the observation group was lower than that in the control group. The duration of use of antibiotics and hospital stay in the observation group were shorter than those in the control group. There were statistically significant differences between the two groups(P<0.05). The results of logistic regression analysis showed that use of prophylactic levofloxacin could effectively reduce the incidence of infections after adjustment for risk grade, gender and age(aOR=2.814, P<0.05). The most common infection in the control group and the observation group was respiratory tract infection, but the incidence of respiratory tract infection in the observation group was lower than that in the control group, and the difference was statistically significant(P<0.05). Conclusion　The use of prophylactic levofloxacin can significantly reduce the incidence rates of BSI, FN, CDI and serious infections in children with ALL during CAT treatment, and reduce the exposure of cephalosporins and macrolides, which is worthy of clinical recommendation.]]> 2023/2/28 12:21:57 TANG Yun-yun, WANG Ning-ling, LIU Kang-kang, et al. 350true ［Abstract］　Objective　To evaluate the efficacy of vertebral bone marrow fat fraction(FF) detected by 3.0T magnetic resonance imaging(MRI) iterative decomposition of water and fat with echo asymmetrical and least-squares estimation quantitation sequence(IDEAL-IQ) in diagnosis of osteoporosis. Methods　Fifty-one healthy people undergoing physical examination in Zhongshan Hospital, Fudan University(Xiamen Branch) from February 2021 to February 2022 were selected, including 25 males and 26 females. Bone mineral density(T value) was measured by dual-energy X-ray absorptiometry(DXA) and vertebral bone marrow FF value was measured by 3.0T MRI IDEAL-IQ on the same day. According to the results of DXA examination, the study subjects were divided into normal bone group(T value≥-1.0 SD, 25 cases) and osteoporosis group(T value≤-2.5 SD, 26 cases). FF value was compared between the two groups, and the correlation between FF value and T value was analyzed. Receiver operating characteristic(ROC) curve was used to evaluate the efficacy of FF value in the diagnosis of osteoporosis. Results　Compared with the normal bone group, the osteoporosis group had older age and larger FF value, and smaller T value, with statistically significant differences between the two groups(P<0.05). The results of Spearman rank correlation showed that FF value was negatively correlated with T value(r_s=-0.642, P<0.001). The results of ROC curve analysis showed that FF value can be used to diagnose osteoporosis［AUC(95%CI)=0.922(0.879-0.965), P<0.001］. The best cutoff value was 50.42%, and its corresponding sensitivity and specificity were 88.10% and 82.60%, respectively. Conclusion　The FF value of vertebral bone marrow detected by 3.0T MRI IDEAL-IQ is negatively correlated with T value, which has the application value in the diagnosis of osteoporosis.]]> 2023/2/28 12:21:57 HUANG Guo-qiang, TANG Qi-ying, WANG Fu-nan, et al. 349true ［Abstract］　Objective　To analyse the clinical efficacy of laparoscopic cystic duct V cutting and molding in treatment of type Ⅱ Mirizzi syndrome. Methods　The clinical data of 6 patients with type Ⅱ Mirizzi syndrome who were admitted to the Department of Hepatobiliary Surgery of Taizhou People′s Hospital from January 2019 to January 2022 were retrospectively analyzed, and the diagnostic methods, the surgical methods, and the short- and long-term treatment effects of the patients were analyzed. Results　All the 6 patients were successfully treated with laparoscopic cystic duct V cutting and molding to repair the biliary duct defects, and decompressed by the support of T tube. After intraoperative biliary endoscopic exploration, there were no biliary tract injury, no postoperative biliary leakage, no recurrence of stones, no occurrence of small gallbladder and no biliary tract stenosis up to now. Conclusion　Laparoscopic cystic duct V cutting and molding has a good therapeutic effect on type Ⅱ Mirizzi syndrome.]]> 2023/2/28 12:21:57 TIAN Ye, CAO Xiao-fei, ZHOU Jing 348true ［Abstract］　Objective　To analyse the clinical manifestations of patent foramen ovale(PFO) in children and the indications of interventional therapy and its therapeutic effect. Methods　A total of 21 pediatric cases of PFO treated by interventional therapy in Linyi People′s Hospital from January 2017 to December 2019 were selected. The patients′ clinical manifestations and the effect of interventional therapy were analyzed. Results　The results of transthoracic echocardiography(TTE) showed that compared with those before intervention, the inner diameter of the right ventricle(RV) and the mediolateral diameter and anteroposterior diameter of the right atrium were decreased at the time of follow-up of 1 week,1 month, 3 months, 6 months and 12 months after surgery, and the differences were statistically significant(P<0.05). The symptoms disappeared in 7 of 9 children with migraine, and the symptoms of chest tightness and palpitation in 6 cases were completely disappeared. Conclusion　The interventional therapy is effective and safe for children with PFO. The operation technique is apt to be used widely and worthy of clinical recommendation.]]> 2023/2/28 12:21:57 SUN Sheng-tao, LI Yin-feng, MA Yun-xia, et al. 347true ［Abstract］　Objective　To explore the effects of obstructive sleep apnea hypopnea syndrome(OSAHS) on maternal and infant outcomes in the pregnant women in the second and third trimesters. Methods　One hundred and fifty-six pregnant women who were admitted to Liuzhou Worker′s Hospital Affiliated to Guangxi Medical University from March 2020 to March 2021 were screened for respiratory and sleep disorders, and according to the screened results, the pregnant women were assigned into the OSAHS group(n=42) and the non-OSAHS group(n=114). The clinical characteristics, maternal and infant outcomes were compared between the two groups. Results　There was no significant difference in the proportion of the patients with a history of hypertension, a history of diabetes, hypothyroidism and family history of OSAHS(P>0.05). The prevalence rates of OSAHS in the pregnant women of different age groups(<25 years old, 25-34 years old and ≥35 years old) at the expected delivery periods were 16.7%, 23.7% and 38.9%, respectively, showing an upward trend. The prevalence rate of OSAHS increased with the increase of body mass index(BMI) in the second and third trimesters of pregnancy. The incidence rates of hypertensive disorder complicating pregnancy(HDCP) in the third trimester, gestational diabetes mellitus(GDM) in the OSAHS group(47.6% and 47.6%, respectively) were significantly higher than those in the non-OSAHS group(8.8% and 29.8%, respectively)(P<0.05), and there were no significant differences in the incidence rates of fetal growth restriction(FGR), oligohydramnios, stillbirth or stillborn foetus, preterm birth, cesarean section and postpartum hemorrhage between the two groups(P>0.05). The incidence of low birth weight infants in the OSAHS group(28.6%) was higher than that in the non-OSAHS group(8.8%)(P<0.05). There was no significant difference in the incidence rate of neonatal asphyxia between the two groups(P>0.05). Conclusion　With the increase of BMI level, the prevalence of OSAHS during pregnancy increases gradually. OSAHS of pregnant women is closely related to the occurrence of HDCP, GDM and low birth weight infants. Therefore, the knowledge of dissemination and screening about OSAHS during pregnancy should be strengthened, and the OSAHS during pregnancy should be improved through guiding the life-style changes of the patients and performing medical intervention when necessary.]]> 2023/2/28 12:21:57 ZHANG Li-min, LIU Li, YANG Jin-li 346true ［Abstract］　Objective　To observe the efficacy of biofeedback(BF) combined with prucalopride in treatment of senile functional constipation(FC). Methods　Eighty-four elderly patients with FC who were admitted to Suzhou Science and Technology Town Hospital from July 2020 to December 2021 were selected and randomly divided into observation group and control group by random number table method, with 42 cases in each group. The observation group was treated with BF therapy combined with prucalopride, while the control group was treated with prucalopride for 6 consecutive weeks. The clinical efficacy, constipation score, frequency of weekly total spontaneous complete bowel movement(SCBM), recurrence rate and the occurrence of adverse reactions were compared between the two groups. Results　Compared with those before treatment, the constipation scores in the observation group and the control group after treatment were decreased, and the frequency of weekly total SCBM was increased, and the differences were statistically significant(P<0.05), and the observation group improved more significantly than the control group(P<0.05). The total effective rate of the observation group was higher than that of the control group, and the difference was statistically significant(83.33% vs 61.90%, χ²=4.850, P=0.028). During 3 months of the follow-up, 5 cases(11.90%) relapsed in the observation group and 12 cases(28.57%) in the control group. There was a statistically significant difference in the recurrence rate between the two groups(χ²=30.612, P<0.001). There was no significant difference in the incidence of adverse reactions between the two groups(7.14% vs 9.52%, χ²=0.000, P=1.000). Conclusion　BF combined with prucalopride can effectively treat FC in the elderly patients, with a low recurrence rate and good safety.]]> 2023/2/28 12:21:57 CHENG Xiu-qin, WANG Liang-jing, PAN Shu-bo, et al. 345true ［Abstract］　Objective　To explore the injury manifestations and severity of mucociliary clearance function on nasal mucosa at molecular level in allergic rhinitis(AR). Methods　The tissue biopsies of inferior turbinate in 40 patients who underwent surgery for deviation of nasal septum in our hospital from March 2021 to March 2022 were selected, including 21 cases of AR patients(AR group, n=21) and 19 cases of non-AR patients(control group, n=19). Immunofluorescence was used to observe and evaluate the markers of mucous secretion—Mucin 5AC(MUC5AC) and the markers of ciliary structure—acetylated alpha-tubulin(acet. α-tubulin) at the protein level. Results　Compared with those in the control group, the total fluorescence intensity(TFI) of MUC5AC was increased by 32.365%, and the length of cilia was shortened by nearly half, and the scores of ciliary shedding were increased by nearly 3 times, and the TFI of cilia was decreased by 24.555% in the AR group. Conclusion　The obvious increase of mucous secretion as well as the increase of diverging, shortening, sparse and deciduous cilia may be the molecular basis leading to the impairment of the rhinal mucociliary clearance function in AR patients, which further aggravates the severity of the symptoms of AR.]]> 2023/2/10 0:00:00 GUO Mu-tao, ZHOU Sui-zi, QIU Qian-hui 344true ［Abstract］　Objective　To compare the application effects of different materials in eardrum repair under otoendoscope. Methods　Seventy-one patients(71 ears) with tympanic membrane perforation who underwent tympanic membrane repair under otoendoscope in Quanzhou First Hospital from January 2020 to December 2021 were selected. According to the different materials used for eardrum repair, the patients were divided into the temporal muscle fascia group(20 cases), the tragus cartilage group(41 cases) and the perichondrium of tragus group(10 cases). The operation time, the length of hospital stay, the postoperative hearing recovery and tympanic membrane healing were compared among the three groups. Results　There were no significant differences in the mean threshold of air conductance, the reduction value of mean air bone conductance and the operation time among the three groups(P>0.05). There was statistically significant difference in the length of hospital stay among the three groups(P<0.05), and the temporal muscle fascia group had the longest hospital stay. Re-perforated tympanic membrane occurred in 2 cases(10.00%, 2/20), 0 cases(0.00%, 0/41) and 3 cases(30.00%, 3/10) in the temporal muscle fascia group, the tragus cartilage group and the perichondrium of tragus group, respectively, with a statistically significant difference among the three groups(χ²=11.425, P=0.003). Conclusion　The application of tragus cartilage has a good postoperative effect on eardrum repair under otoendoscope, which can reduce the rate of postoperative re-perforation, and the surgery is easy to operate and extend the application.]]> 2023/2/10 17:50:33 ZHANG Xiao-dong, NIAN Zhong-zhu, LIN Wen, et al. 343true ［Abstract］　Objective　To investigate the in-vitro activity of Mango(Mangifera indica L.) leaf extract against human immunodeficiency virus 1(HIV-1), and to provide reference for the research and development of Traditional Chinese Medicine resources for the treatment of acquired immune deficiency syndrome(AIDS). Methods　The effects of intervention drugs on the activity of TZM-bl cells and MT-2 cells were evaluated by adenosine triphosphate(ATP) assay. Two cell-virus infection models of TZM-bl-HIV-1_ⅢB and MT-2-HIV-1_ⅢB were constructed, and the virus activity was detected by luciferase activity detection reagent, so as to evaluate the anti-HIV-1 activity of intervention drugs. The inhibition of Mango leaf extract on cytopathic effect(CPE) in MT-2-HIV-1_ⅢB cell system was observed. Half toxic concentration(CC₅₀), half inhibitory concentration(IC₅₀) and selection index(SI) of Mango leaf extract were calculated. Results　In the TZM-bl-HIV-1_ⅢB and MT-2-HIV-1_ⅢB cell-virus infection models, Mango leaf extract showed anti-HIV-1 activity in a dose-dependent manner. In the TZM-bl-HIV-1_ⅢB model, the CC₅₀, IC₅₀ and SI of Mango leaf extract were (320.00±29.44)μg/ml, (8.86±0.26)μg/ml and 36.14, respectively. In the MT-2-HIV-1_ⅢB model, the CC₅₀, IC₅₀ and SI of Mango leaf extract were (174.13±22.36)μg/ml, (15.23±9.99)μg/ml and 11.44, respectively. Conclusion　Mango leaf extract has low cytotoxicity in vitro and significant anti-HIV-1 activity, which has potential development and utilization value.]]> 2023/2/10 0:00:00 WANG Qian, YE Li, WANG Jie, et al. 342true ［Abstract］　Objective　To observe the clinical efficacy of press-needle combined with Traditional Chinese Medicine in treatment of chronic rhinitis with heat accumulation in the lung channel. Methods　Sixty patients suffering from chronic rhinitis with heat accumulation in the lung channel who were admitted to Department of Otolaryngology, Affiliated Hospital of Nanjing University of Chinese Medicine from April 2021 to April 2022 were selected and divided into an observation group and a control group by random number table method, with 30 cases in each group. The observation group was treated with press-needle combined with oral Traditional Chinese Medicine, while the control group was treated with budesonide nasal spray. Both of the two groups were treated continuously for 28 days. The symptoms and signs scores were compared between the two groups before treatment and on the 14th day and 28th day after treatment, and the clinical efficacy was evaluated in the two groups. Results　The symptoms and signs scores showed a downward trend in the two groups after treatment, and 14 and 28 days after treatment, the scores in the two groups were significantly lower than those in the same group before treatment(P<0.05), but there were no significant differences between the two groups(P>0.05). The clinical effects of the two groups were similar, and there was no significant difference in the total effective rate between the two groups(90.00% vs 93.33%, P>0.05). Conclusion　Press-needle combined with Traditional Chinese Medicine has a good clinical efficacy in treatment of chronic rhinitis with heat accumulation in the lung channel.]]> 2023/2/10 17:50:33 QIU Xin-ying, XUE Shan-shan, SUN Xin-chen, et al. 341true ［Abstract］　Objective　To investigate the expression and clinical significance of hsa_circ_0001785 in serum of breast cancer patients. Methods　Forty-three patients with breast cancer(the breast cancer group), and 54 patients with benign breast tumor(the benign tumor group) who were admitted to the Department of Thyroid and Breast Surgery of the Fifth Affiliated Hospital of Guangzhou Medical University from September 2021 to March 2022 were selected, and 45 healthy subjects who received physical examination were selected as the healthy control group. The relative expression level of hsa_circ_0001785 in serum was detected by quantitative real-time polymerase chain reaction(qPCR) and was compared among the three groups. The correlation between the expression level of hsa_circ_0001785 and the clinicopathologic features of breast cancer patients were analyzed. The diagnostic efficiency of hsa_circ_0001785 for breast cancer was analyzed by receiver operating characteristic(ROC) curve. Results　The expression level of serum hsa_circ_0001785 in the breast cancer group was significantly higher than that in the benign tumor group and the healthy control group(P<0.05), but there was no significant difference between the benign tumor group and the healthy control group(P>0.05). The results of ROC curve analysis showed that serum hsa_circ_0001785 had the application value for diagnosis of breast cancer［area under the curve(AUC)=0.780, P<0.05］, and the diagnostic efficiency of serum hsa_circ_0001785 was better than that of carcinoembryonic antigen(CEA) and cancer antigen 125(CA125). The expression level of serum hsa_circ_0001785 in the patients with breast cancer was not significantly associated with their age, tumor type, TNM stage, lymph node involvement, distant metastasis, estrogen receptor(ER), progesterone receptor(PR) and human epidermal growth factor receptor 2(HER2)(P>0.05). Conclusion　Hsa_circ_0001785 is highly expressed in serum of breast cancer patients and has potential as a molecular marker for diagnosis of breast cancer.]]> 2023/2/10 17:50:33 ZHUANG Xiao-xu, ZENG Yuan-xian, XIAN Zhong-ren, et al. 340true ［Abstract］　Objective　To investigate the analgesic effects of erector spinae plane block(ESPB) after thoracoscopic lobectomy by the deep surface of erector spinae muscles block approach and the rhomboid muscle and erector spine muscle space block approach, so as to provide a basis for clinical selection of better ESPB pathway. Methods　From December 1, 2021 to May 30, 2022, 90 patients who underwent elective surgery of video-assisted thoracoscopic lobectomy under plugging device bronchial intubation and general anesthesia in Yantai Yuhuangding Hospital were selected. The patients were divided into group E, group R and group C by random number table method, with 30 cases in each group. The deep surface of erector spinae muscles block approach was used in the group E, and the rhomboid muscle and erector spine muscle space block approach was used in the group R, and the group C was used as control group without any operation of ESPB. The Visual Analogue Scale(VAS) scores were compared among the three groups at resting state and active or coughing state 6 hours(T₁), 12 hours(T₂), 24 hours(T₃) and 48 hours(T₄) after operation. The postoperative rescue analgesia and the occurrence of adverse reactions were compared among the three groups. Results　At the time points from T₁ to T₄, the VAS scores showed a trend of increase first and then decrease in the three groups at resting state and active or coughing state. At the time points of T₁, T₂ and T₃, the VAS scores in the group E at resting state and active or coughing state were significantly lower than those in the group R and the group C(P<0.05). At the time point of T₁, the VAS scores of the group R were lower than those of the group C at resting state and active or coughing state, and at the time point of T₂, the VAS scores of the group R were significantly lower than those of the group C only at active or coughing state, and the differences were significant(P<0.05). There were no significant differences in VAS scores at the time point of T₄ among the three groups(P>0.05). Compared with that in the group R and the group C, the frequency of postoperative rescue analgesia in the group E was less and the scores of analgesia satisfaction in the group E were higher than those in the group R and the group C, and the differences were statistically significant(P<0.05). There were 11 cases of nausea and vomiting in the group E(36.67%), 12 cases of nausea and vomiting in the group R(40.00%) and 17 cases of nausea and vomiting in the group C(56.57%), and the difference was not statistically significant among the three groups(χ²=2.790, P=0.248). Conclusion　ESPB of the two approaches can relieve the pain of patients after thoracoscopic lobectomy to some extent, and can reduce the use of postoperative analgesics with less complications, of which the analgesic effect of the deep surface of erector spinae muscles block approach is better than that of the rhomboid muscle and erector spine muscle space block approach 24 hours after operation.]]> 2023/2/10 17:50:33 WANG Jie, LI Ke-zhong 339true ［Abstract］　Objective　To study the application effect of digital preoperative planning on the placement of pedicle screws in patients with severe lumbar vertebra degeneration. Methods　Forty-seven patients who received lumbar pedicle screw placement in Wuhan Orthopaedics Hospital of Integrated Traditional Chinese and Western Medicine from May 2018 to May 2020 were selected, including 24 patients with lumbar spinal stenosis and 23 patients with degenerative lumbar spondylolisthesis. The patients were divided into the observation group(with digital preoperative planning, 22 cases) and the control group(without digital preoperative planning, 25 cases) according to whether they received digital preoperative planning. The patients in both groups received transforaminal lumbar interbody fusion(TLIF). The time of the placement of pedicle screws, the blood loss during the time of the placement of pedicle screws, the time of intraoperative fluoroscopy and the accuracy of the placement of pedicle screws were compared between the two groups. Results　The operation was successfully completed in the patients of the two groups, and there were no postoperative complications such as nerve injury, cerebrospinal fluid leakage and incision infection. A total of 152 nails were placed in 22 patients in the observation group and 172 nails were placed in 25 patients in the control group. The time of per nail placement and the time of intraoperative fluoroscopy in the observation group was shorter than that in the control group, and the blood loss during the time of per nail placement was less than that in the control group, with statistically significant differences between the two groups(P<0.05). The overall accuracy of nail placement in the observation group was significantly better than that in the control group(P<0.05). Conclusion　For patients with severe lumbar joint degeneration, digital preoperative planning can provide reference for nail placement, which is helpful to shorten intraoperative time of nail placement, reduce intraoperative blood loss and fluoroscopy time, and improve the accuracy of nail placement.]]> 2023/2/10 17:50:33 WANG Xiao-zhen, ZHANG Xiao-tong, WU Zhe-dong, et al. 338true ［Abstract］　Objective　To evaluate the clinical effect of Dyad Flow self-adhesive flow resin for preventive resin restoration(PRR) of pit and fissure caries in children′s permanent molars. Methods　Seventy-eight children with pit and fissure caries in the first permanent molar(184 teeth in total) who were treated in the Department of Stomatology of the People′s Hospital of Guangxi Zhuang Autonomous Region from June 2016 to June 2018 were selected. The patients ranged in age from 6 to 14 years, and were all prepared with PRR technology. The patients were divided into observation group and control group by random number table method, with 39 cases(95 teeth) in the observation group who were filled with Dyad Flow self-adhesive flow resin and 39 cases(89 teeth) in the control group who were filled with Beautifil Flow Plus F03 reinforced flowable composite. The time consuming of material filling operations in the two groups was recorded. The outpatient follow-up was performed on the patients 6, 12, 24, and 36 months after treatment. The evaluation criteria of modified United States Public Health Service(USPHS) was used to evaluate the treatment effects of the two groups, and the retention of filling materials was compared between the two groups. Results　There were no significant differences between the two groups in terms of filling integrity, surface texture and secondary caries 6, 12, 24, and 36 months after treatment(P>0.05). The edge coloration in the control group was significantly better than that in the observation group 36 months after treatment(P<0.05). The results of survival analysis showed that the material retention of the control group was slightly better than that of the observation group, but the difference was not statistically significant between the two groups(log-rank test: χ²=3.565, P=0.059). The material-filling operation time in the observation group was shorter than that in the control group, and the difference was statistically significant［(59.52±0.67)s vs (137.56±0.73)s; t=78.557, P<0.001］. Conclusion　Dyad Flow self-adhesive flow resin and Beautifil Flow Plus F03 reinforced flowable composite have similar effects on PRR of pit and fissure caries in permanent molars, but Dyad Flow self-adhesive flow resin has shorter filling operation time and is more suitable for children.]]> 2023/2/10 17:50:33 SHI Chun-mei, HUANG Cheng-cheng, LUO Ju-fen, et al. 337true ［Abstract］　Objective　To investigate the efficacy of intravitreal injection of anti-vascular endothelial growth factor(VEGF) in treatment of wet age-related macular degeneration(wAMD) and its effect on inflammatory factors of aqueous humor. Methods　One hundred and twelve patients with wAMD who were admitted to the First Affiliated Hospital of Henan University of Science and Technology from January 2018 to September 2020 were selected and divided into observation group and control group by random number table method, with 56 cases in each group. The observation group was treated with intravitreal injection of anti-VEGF(ranibizumab), and the control group received photodynamic therapy(PDT). The visual acuity improvement after treatment was compared between the two groups. The intraocular pressure(IOP), choroidal neovascularization(CNV) area, central macular thickness(CMT) and the levels of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and interleukin-10(IL-10) in aqueous humor were compared between the two groups before and 3 months after treatment. The occurrence of complications was observed in the two groups. Results　The level of IOP, the CNV area and the CMT value in the two groups were significantly decreased 3 months after treatment compared with those before treatment(P<0.05), but there were no significant differences between the two groups(P>0.05). The levels of TNF-α, IL-6 and IL-10 in the aqueous humor of the control group had no significant changes compared with those before treatment(P>0.05). The levels of TNF-α and IL-6 in the aqueous humor of the observation group were significantly lower than those before treatment(P<0.05), and lower than those of the control group, and the differences were statistically significant(P<0.05). Three months after treatment, there was no significant difference in visual acuity improvement between the two groups(P>0.05). Six months after treatment, the visual acuity improvement in the observation group was significantly better than that in the control group(P<0.05). No serious complications such as retinal detachment occurred in the two groups, and there was no significant difference in the incidence of complications(5.36% vs 7.14%; P>0.05). Conclusion　Compared with PDT, anti-VEGF intravitreal injection in the treatment of wAMD can more effectively control the disease, reduce the inflammatory reaction of aqueous humor, and improve the visual acuity of the patients.]]> 2023/2/10 17:50:33 XIE Qi-lian, WEI Jing, SHANG Li-xiao 336true ［Abstract］　Objective　To investigate the diagnostic value of Vesical Imaging-Reporting and Data System(VI-RADS) score for muscle invasive bladder cancer. Methods　Fifty-nine bladder cancer patients(60 lesions) admitted to Chuzhou Hospital Affiliated to Anhui Medical University from May 2018 to July 2022 were selected, all of which were urothelial carcinoma. All the patients underwent multiparameter magnetic resonance imaging(mp-MRI) examination. Two radiologists independently scored the examined images of the patients according to the VI-RADS scoring standard, and compared the imaging results with the pathological results. The receiver operating characteristic(ROC) curve was used to analyze the diagnostic efficacy of VI-RADS score for muscle invasive bladder cancer. Results　Among the 60 lesions, there were 38 cases of non-muscle invasive bladder cancer(NMIBC) and 22 cases of muscle invasive bladder cancer(MIBC). The VI-RADS scores were 1 point in 6 cases, 2 points in 21 cases, 3 points in 17 cases, 4 points in 12 cases, and 5 points in 4 cases. The VI-RADS score results from the two radiologists were in good agreement(Kappa=0.755, P<0.001). The results of ROC curve analysis showed that VI-RADS score could effectively diagnose muscle invasive bladder cancer［area under the curve(AUC)(95%CI)=0.880(0.790-0.971), P<0.001］. The optimal cut-off value was 3.5, with a sensitivity of 63.64% and a specificity of 94.74%. Conclusion　VI-RADS score has good diagnostic efficacy for muscle invasive bladder cancer, which is worthy of clinical promotion.]]> 2023/2/10 17:50:33 CHU Yu-lu, MENG Qing-tao, WANG Xue-qiang, et al. 335true ［Abstract］　Objective　To investigate the application value of preoperative Capillary Index Score(CIS) in evaluating the prognosis of patients undergoing thrombectomy for anterior circulation large vessel occlusion(LVO). Methods　The clinical data of 106 patients with acute anterior circulation LVO who were admitted to the Second Affiliated Hospital of Guilin Medical University and underwent mechanical thrombectomy from January 2016 to July 2021 were selected. CIS was calculated according to the preoperative cerebral angiography data, including 44 cases of poor CIS(pCIS, CIS: 0-1 points, pCIS group) and 62 cases of favorable CIS(fCIS, CIS: 2-3 points, fCIS group). The patients with poor prognosis［modified Rankin Scale(mRS) score>2 points］ and the risk factors of malignant cerebral edema 90 days after mechanical thrombectomy were analyzed. Results　Compared with the fCIS group, the pCIS group had a smaller proportion of patients with hypertension and coronary heart disease, lower Alberta Stroke Program Early CT Score(ASPECTS), and higher National Institute of Health Stroke Scale(NIHSS) score at admission, greater proportion of patients on whom remedial measures were taken during the operation, higher incidence of postoperative malignant cerebral edema, and higher rate of poor prognosis and mortality 90 days after surgery, and the differences were statistically significant(P<0.05). The results of binary logistic regression analysis showed that high NIHSS score at admission［OR(95%CI)=1.214(1.102, 1.338)］ and pCIS ［OR(95%CI)=4.924(1.780, 13.620)］ were the risk factors for poor prognosis 90 days after mechanical thrombectomy(P<0.05). pCIS［OR(95%CI)=21.670(6.593, 71.226)］, modified thrombolysis in cerebral infarction(mTICI) grade 0～2a ［OR(95%CI)=50.334(3.865, 655.525)］ and time from ictus to revascularization(TIR)>4.5 h［OR(95%CI)=5.557(1.392, 22.180)］ were the risk factors for postoperative malignant cerebral edema(P<0.05). Conclusion　Anterior circulation LVO patients with pCIS have a poor prognosis and a high incidence of malignant cerebral edema. Therefore, mechanical thrombectomy should be carefully considered.]]> 2023/2/10 17:50:33 YANG Ming-hao, TANG Jian-xun, HU Li-gang, et al. 334true ［Abstract］　Objective　To investigate the dynamic changes and clinical significance of a disintegrin and metalloproteinase with a thrombospondin type 1 motif member 13(ADAMTS13) after intravenous thrombolysis in patients with acute cerebral infarction(ACI). Methods　The clinical data of eighty-three patients with mild and moderate ACI who received intravenous thrombolytic therapy with recombinant tissue plasminogen activator(rt-PA) in the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2020 to December 2021 were collected. According to the National Institutes of Health Stroke Scale(NIHSS) scores of the patients at admission, they were divided into mild group(NIHSS scores≤5 points, 44 cases) and moderate group(5 pointsResults　Compared with those in the mild group and the moderate group before thrombolysis, the ADAMTS13 levels in the mild group and the moderate group showed a downward trend after thrombolysis, and there was no significant difference in ADAMTS13 level between the two groups at each time point(P>0.05). The results of multivariate logistic regression analysis showed that the ADAMTS13 level before thrombolysis was not significantly correlated with the severity of the patients′ condition at admission［OR(95%CI)=0.280(0.036-2.196), P=0.226］, and a higher ADAMTS13 level 72 hours after thrombolysis was a protective factor against adverse outcomes in the ACI patients 90 days after treatment［OR(95%CI)=0.556(0.005-0.991), P=0.049］. The results of receiver operating characteristic(ROC) curve analysis indicated that the ADAMTS13 level 72 hours after thrombolytic therapy had the application value of predicting 90-day prognosis of the ACI patients after treatment［area under the curve(AUC)(95%CI)=0.693(0.547-0.839), P=0.021］, with an optimal cut-off value of 5.860, and the corresponding sensitivity and specificity were 76.90% and 56.50%, respectively. Conclusion　Serum ADAMTS13 levels in ACI patients show a downward trend in the early stage after intravenous thrombolysis, and a higher ADAMTS13 level 72 hours after thrombolysis is a protective factor that inhibits the adverse prognosis of the ACI patients 90 days after treatment.]]> 2023/12/30 8:18:06 JIANG Bing-jian, LU Huan-huan, HUANG Shu-xuan, et al. 333true ［Abstract］　Objective　To investigate the application value of contrast-enhanced ultrasonography(CEUS) combined with fecal calprotectin(FC) detection in assessing the activity of Crohn′s disease(CD). Methods　The clinical data of 45 CD patients who were admitted to the University of Hong Kong-Shenzhen Hospital from January 2021 to June 2023 were retrospectively analyzed. According to CD activity index(CDAI) scores, the patients were divided into remission group(<150 points, 18 cases) and active stage group(150-450 points, 27 cases). All the patients underwent routine intestinal ultrasonography and CEUS, and FC levels were detected by double antibody sandwich method. Peak intensity(PI), area under the curve(AUC) and FC level of the CEUS indicators were analyzed using receiver operating characteristic(ROC) curve to assess the activity of CD. Results　The indicators of PI and AUC, and the FC level in the remission group were lower than those in the active stage group, and the differences were statistically significant(P<0.05). The results of Pearson correlation analysis showed that CDAI score was positively correlated with the indicators of PI(r=0.506, P<0.001) and AUC(r=0.544, P<0.001), and the FC(r=0.364, P=0.014) level. The results of ROC curve analysis showed that the indicators of PI and AUC, and the FC level all had application value in assessing the activity of CD(P<0.05), and the combined assessing efficacy of the three indicators was further improved［AUC(95%CI)=0.908(0.815-1.000), P<0.001］, and the sensitivity and the specificity were 96.30% and 83.33%, respectively. Conclusion　CEUS combined with FC detection can effectively assess the activity of CD, which helps clinicians judge the patients′ conditions and timely adjust the appropriate treatment plans.]]> 2023/12/30 8:18:06 CHEN Jing, DONG Zhen-yu, YANG Dong-ye, et al. 332true ［Abstract］　Objective　To screen the diagnostic marker genes of rheumatoid arthritis(RA) and analyze the possible immune infiltration mechanism based on bioinformatics and machine learning, and to provide reference for the clinical treatment of RA. Methods　The gene expression profiles were downloaded from the Gene Expression Omnibus(GEO) database. GSE55235 and GSE77298 were used as the combined chip training set, and GSE55457 was used as the independent validation dataset. The differentially expressed genes(DEGs) were screened using R software, and Gene Ontology(GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analysis were performed for these DEGs. Three machine learning algorithms were further applied to screen the diagnostic genes and perform the external validation and receiver operating characteristic(ROC) curve analysis. Finally, the xCell method was used to calculate the infiltration of immune cell types in the RA. The infiltration of immune cells in RA was analyzed by using xCell algorithm. Results　A total of 704 DEGs of RA were screened. The results of enrichment analysis revealed that these DEGs were mainly involved in some related immune functions, such as leukocyte-mediated immunity, activation of immune response, and leukocyte migration, and some inflammatory pathways, such as chemokine signaling pathway, Leishmaniasis and Rheumatoid arthritis. Four diagnostic genes, including C-X-C motif chemokine ligand 13(CXCL13), leucine rich repeat containing 15(LRRC15), syndecan 1(SDC-1) and Y-box binding protein 3(YBX3), were screened using machine learning. The results of the immune infiltration analysis showed that the expression levels of B cells, CD4⁺ T cells, dendritic cells and monocytes were significantly up-regulated in RA. Conclusion　Multiple genes and pathways are involved in the occurrence and development of RA. CXCL13, LRRC15, SDC-1 and YBX3 may be the potential biomarkers for the diagnosis of RA. Moreover, B cells, CD4⁺ T cells, dendritic cells and monocytes may play an important role in the occurrence of RA.]]> 2023/12/30 0:00:00 LI Ling-qin, ZHOU Rui-jiao, ZHANG Yan-ni, et al. 331true ［Abstract］　Objective　To analyze the correlation of serum long non-coding RNA(lncRNA) ROR and micro ribonucleic acid-145(miR-145) levels with the occurrence of atrial fibrillation(AF) in chronic heart failure(CHF) patients. Methods　One hundred and eight CHF patients admitted to Handan First Hospital from March 2019 to December 2022 were recruited. The patients were divided into AF group(n=46) and non-AF group(n=62) according to the occurrence of AF. During the same period, 50 healthy individuals who matched gender and age with the CHF patients were included as the control group. Quantitative real-time polymerase chain reaction(qRT-PCR) was used to detect the expression levels of serum lncRNA ROR and miR-145. Pearson correlation analysis was used to explore the correlation of serum lncRNA ROR and miR-145 expression levels with cardiac function indicators. Multivariate logistic regression was used to analyze the influencing factors of AF occurrence in the CHF patients. The predictive efficacy of serum lncRNA ROR and miR-145 in the occurrence of AF was analyzed for the CHF patients using receiver operating characteristic(ROC) curve. Results　Compared with those in the control group, the levels of lncRNA ROR in the non-AF group and the AF group were up-regulated, and the AF group had higher levels of lncRNA ROR than the non-AF group, with a statistically significant difference(P<0.05). Compared with those in the health group, the levels of miR-145 in the non-AF group and the AF group were down-regulated, and the AF group had lower levels of miR-145 than the non-AF group, with a statistically significant difference(P<0.05). The results of Pearson correlation analysis showed that serum lncRNA ROR levels were positively correlated with left atrial diameter(LAD), left ventricular end diastolic diameter(LVEDD), and left ventricular end systolic diameter(LVESD)(P<0.05), and negatively correlated with left ventricular ejection fraction(LVEF)(P<0.05). The level of miR-145 was negatively correlated with LAD, LVEDD and LVESD(P<0.05), and positively correlated with LVEF(P<0.05). The results of multivariate logistic regression analysis showed that New York Heart Association(NYHA) classification cardiac function class Ⅳ, decreased LVEF, and up-regulated lncRNA ROR level were independent risk factors for promoting the occurrence of AF in the CHF patients(P<0.05), while up-regulated miR-145 level was an independent protective factor for inhibiting the occurrence of AF in the CHF patients(P<0.05). The results of ROC curve analysis showed that the expression levels of serum lncRNA ROR and miR-145 could effectively predict the occurrence of AF in the CHF patients, and the combination of the two indicators had better predictive efficacy［AUC(95%CI)=0.814(0.730-0.897), P<0.001］, and the sensitivity and the specificity were 89.80% and 72.00%, respectively. Conclusion　Serum lncRNA ROR and miR-145 are closely related to the occurrence of AF in CHF patients. The combined detection of the two indicators has higher predictive value for the occurrence of AF.]]> 2023/12/30 8:18:06 WU Hai-tao, LI Hong-jing, MIAO Yan-li, et al. 330true ［Abstract］　Objective　To explore the protective mechanism of human adipose mesenchymal stem cell-derived exosomes(Exosome-HADSCs) combined with astragalus polysaccharides(APS) against myocardial injury. Methods　Human adipose mesenchymal stem cells(HADSCs) were cultured in vitro, and exosomes were extracted and identified using electron microscopy and Western blot. Control group, Exo group, APS group and Exo+APS group were set up. The effects of Exosome-HADSCs combined with APS on vascular formation and cell migration of human umbilical vein endothelial cells(HUVECs) were analyzed using the angiogenesis test and the scratch test. In the AC16 cardiomyocyte injury model, control group, cobalt chloride(CoCl₂) group, CoCl₂+Exo group, CoCl₂+APS group and CoCl₂+Exo+APS group were set up to compare the cell survival rate, apoptosis rate and the expression levels of apoptosis-related proteins among all the groups. Results　Compared with that in the control group, the number of vascular mesh formation in HUVECs in the Exo+APS group was significantly increased(P<0.05), and the number of vascular mesh formation in the Exo+APS group was larger than that in the Exo group and the APS group, and the differences were statistically significant(P<0.05). Compared with that in the control group, the cell migration rate in the Exo+APS group was increased, and the cell migration rate in the Exo+APS group was higher than that in the Exo group and the APS group,and the differences were statistically significant(P<0.05). Exosome-HADSCs combined with APS could significantly improve the survival rate of AC16 cells damaged by CoCl₂ and decrease the apoptosis rate of AC16 cells damaged by CoCl₂, and the effects of Exosome-HADSCs combined with APS were more significant than the effects of Exosome-HADSCs and the effects of APS alone(P<0.05). Compared with those in the CoCl₂ group, the expressions of apoptosis-related proteins B-cell lymphoma-2 associated x protein(Bax) and cysteine aspartate specific protease-3(caspase-3) in the CoCl₂+Exo+APS group were significantly decreased(P<0.05), and the expression of anti-apoptotic protein B-cell lymphoma-2(Bcl-2) was significantly increased(P<0.05), and the expression levels of phosphatidylinositol-3-kinase(PI3K), phosphated phosphatidylinositol-3-kinase(p-PI3K) and serine-threonine kinase(Akt) were significantly increased(P<0.05). Conclusion　Exosome-HADSCs combined with APS have protective effects on myocardial injury, which may be related to promoting angiogenesis and endothelial cell migration, improving cell survival rate, and reducing myocardial cell apoptosis through PI3K/Akt signaling pathway.]]> 2023/12/30 8:18:06 GUO Cai-ru, LIU Xin-xin, NIU Yu-jie, et al. 329true ［Abstract］　Objective　To investigate the expressions of orphan receptor G protein coupled receptor 15(GPR15) and high mobility group proteome A1(HMGA1) in colorectal cancer(CRC) tissues and their clinical prognostic value. Methods　Eighty-nine CRC patients who underwent radical surgery in Affiliated Hospital of Yangzhou University from January 2019 to January 2020 were selected, and their intraoperative CRC tissues and the cancer-adjacent tissues were collected. Quantitative real-time polymerase chain reaction(qRT-PCR) and immunohistochemistry staining were used to detect the expressions of GPR15 and HMGA1 in the tissues. The correlation between the expressions of GPR15 and HMGA1 in the CRC tissues and the clinicopathological characteristics of the patients was analyzed. The Kaplan-Meier method was used to analyze the correlation between the expressions of GPR15 and HMGA1 in the CRC tissues and the patients′ survival prognosis. Cox regression was used to analyze the influencing factors of prognosis in the CRC patients. Results　The relative expression levels of GPR15 and HMGA1 mRNA in the CRC tissues were significantly higher than those in the cancer-adjacent tissues(P<0.05). The positive expression rates of GPR15 and HMGA1 proteins in the CRC tissues were significantly higher than those in the cancer-adjacent tissues(P<0.05). The positive expression rates of GPR15 and HMGA1 in the CRC tissues with TNM stage Ⅲ, low differentiation, and combined lymph node metastasis were higher than those in the CRC tissues with TNM stage Ⅰ-Ⅱ, high differentiation and non-lymph node metastasis, respectively(P<0.05). The survival prognosis of the GPR15 negative group was significantly better than that of the GPR15 positive group(log-rank test: χ²=9.124, P=0.003). The survival prognosis of the HMGA1 negative group was significantly better than that of the HMGA1 positive group(log-rank test: χ²=10.140, P=0.001). Tumor TNM stage Ⅲ, low differentiation, combined lymph node metastasis, GPR15 positive and HMGA1 positive were the independent risk factors promoting poor prognosis of the CRC patients(P<0.05). Conclusion　The expression levels of GPR15 and HMGA1 are increased in CRC tissues, which are closely related to adverse clinicopathological characteristics of CRC patients. GPR15 and HMGA1 can be used as new tumor markers to evaluate the prognosis of the CRC patients.]]> 2023/12/30 8:18:06 WANG Peng-hui, HU Jie, PAN Rong-yan 328true ［Abstract］　Objective　To analyze the influencing factors of acute radiation-induced skin injury caused by hypofractionated radiotherapy after breast-conserving surgery, and to construct its risk prediction model. Methods　The clinical data of 170 patients with breast cancer who underwent breast-conserving surgery for breast cancer in Tangshan People′s Hospital from October 2020 to September 2022 were selected, and the 170 patients were divided into model group(119 cases) and validation group(51 cases) according to a ratio of 7∶3. According to the occurrence of acute radiation-induced skin injury, the patients of the model group were further divided into injury group(89 cases) and non-injury group(30 cases). The clinical data were compared between the injury group and the non-injury group, and the risk factors were analyzed using multivariate logistic regression, and the risk prediction model was constructed using nomogram. Results　The results of multivariate logistic regression analysis showed that body mass index(BMI)≥28 kg/m², history of diabetes, T stage of tumor at T₂, triple negative breast cancer, the form of radiotherapy being sequential tumor bed electron line supplement radiotherapy, higher CD4⁺ T lymphocyte apoptosis rate and hemoglobin(Hb) level were independent risk factors for the patients with acute radiation-induced skin injury(P<0.05). Based on these indicators, a nomogram risk prediction model was constructed. The results of receiver operating characteristic(ROC) curve analysis showed that the area under the curve(AUC) for predicting the risk of acute radiation-induced skin injury was 0.942(95%CI: 0.890-0.974) for the model group, with the sensitivity of 97.14% and the specificity of 86.24%, and AUC for predicting the risk of acute radiation-induced skin injury was 0.926(95%CI: 0.880-0.966) for the validation group, with the sensitivity of 86.36%, and the specificity of 90.40%. Conclusion　BMI, history of diabetes, T stage of tumor, triple negative breast cancer, the form of radiotherapy, CD4⁺ T lymphocyte apoptosis rate and Hb level are the factors affecting acute radiation-induced skin injury caused by hypofractionated radiotherapy after breast-conserving surgery. The risk prediction model of nomogram constructed based on this basis has good clinical application value.]]> 2023/12/30 0:00:00 WANG Jian-ting, SHAO Rui-yu, WANG Jing, et al. 327true ［Abstract］　Objective　To investigate the clinical application value of orbital color Doppler imaging(OCDI) in diagnosis of central retinal artery occlusion. Methods　The clinical data of 34 patients(34 eyes) with central retinal artery occlusion who were admitted to the Second Affiliated Hospital of Army Medical University from January 2015 to September 2021 were selected, including 15 patients(15 eyes) aged <60 years and 19 patients(19 eyes) aged ≥60 years. Other 30 healthy subjects(30 eyes) during the same period were included as the control group. The hemodynamics indicators of the central retinal artery in the affected eyes and the healthy eyes of the patients were detected using OCDI, including peak systolic velocity(PSV), end diastolic velocity(EDV), resistance index(RI) and pulsatility index(PI). Kappa consistency test was used to analyze the consistency between the findings of fluorescein fundus angiography(FFA) and the findings of OCDI. The clinical features of the patients with central retinal artery occlusion whose OCDI was negative were analyzed. Results　The PSV and EDV levels in the affected eyes of the patients with central retinal artery occlusion were lower than those in the healthy eyes, and the RI and PI levels in the affected eyes were higher than those in the healthy eyes, and the differences were statistically significant(P<0.05). The PI level in the affected eyes of the central retinal artery occlusion patients aged ≥60 years was significantly higher than that in the affected eyes of the patients in the control group(P<0.05), but there was no significant difference in the PI level between the affected eyes and the healthy eyes of the central retinal artery occlusion patients(P>0.05). There were no significant differences in the indicators of PSV, EDV and RI among the affected eyes, the healthy eyes and the control group(P>0.05). For the central retinal artery occlusion patients aged <60 years, the levels of PSV and EDV in the affected eyes were significantly lower than those in the healthy eyes and the control group(P<0.05), and the levels of PI and RI were higher than those in the control group(P<0.05), but there was no statistically significant difference between the patients and the healthy eyes(P>0.05). OCDI and FFA had good consistency in the diagnosis of central retinal artery occlusion(Kappa=0.807, P<0.001). The time from onset to visiting the doctor in the patients with negative OCDI diagnosis was later than that of the patients with positive OCDI diagnosis(P<0.05), and the difference was statistically significant(P<0.05). There were no significant differences in PSV, EDV and RI between the affected eyes and the healthy eyes of the patients with negative OCDI diagnosis(P>0.05). Conclusion　The hemodynamics of central retinal artery in patients with central retinal artery occlusion are significantly changed. OCDI examination is helpful for early diagnosis of central retinal artery occlusion.]]> 2023/12/30 8:18:06 HUANG Chan-juan, LI Pei-jing, YAUN Rong-di 326true ［Abstract］　Objective　To study the relationship between serum deoxyribonucleic acid(DNA) methyltransferase 1(DNMT1) and long non-coding ribonucleic acid(RNA) human maternally expressed gene 3(lncRNA MEG3) expression levels and Parkinson′s disease(PD). Methods　One hundred and six patients with PD who were admitted to the Department of Neurology of Qinghai Red Cross Hospital from March 2020 to January 2023 were recruited as the PD group, and 103 cases undergoing healthy physical examination during the same period were selected as the control group. The clinical data were compared between the two groups to analyze the correlation between the serum DNMT1 and lncRNA MEG3 expression levels and clinical indicators in the PD patients. Multivariate logistic regression was used to analyze the factors affecting the occurrence of PD, and receiver operating characteristic(ROC) curve was used to evaluate the diagnostic value of the serum DNMT1 and lncRNA MEG3 levels in PD. Results　The serum DNMT1 level in the PD group was higher than that in the control group, and the total cholesterol(TC), triglyceride(TG), low density lipoprotein-cholesterol(LDL-C) and lncRNA MEG3 levels in the PD group were lower than those in the control group, and the differences were statistically significant(P<0.05). The results of Pearson correlation analysis showed that the serum DNMT1 level of the PD patients was negatively correlated with TC, TG and LDL-C levels(P<0.05), and was positively correlated with the Unified Parkinson′s Disease Rating Scale(UPDRS) Ⅰ scores, UPDRS Ⅱ scores, UPDRS Ⅲ scores and Hoehn-Yahr(H-Y) staging(P<0.05). The lncRNA MEG3 level was positively correlated with TC, TG and LDL-C levels(P<0.05), and was negatively correlated with UPDRS Ⅰ scores, UPDRS Ⅱ scores, UPDRS Ⅲ scores and H-Y staging(P<0.05). The results of multivariate logistic regression analysis showed that higher serum DNMT1 level was an independent risk factor for promoting PD occurrence(P<0.05), and higher lncRNA MEG3 level was an independent protective factor for inhibiting PD occurrence(P<0.05). The results of ROC curve analysis showed that the serum DNMT1 and lncRNA MEG3 levels had application value in the diagnosis of PD(P<0.05), and the combination of the two indicators could further improve the diagnostic efficiency［AUC(95%CI)=0.906(0.865-0.947)］. The sensitivity and specificity were 80.21% and 89.34%, respectively. Conclusion　Serum DNMT1 expression is high in PD patients, and lncRNA MEG3 expression is low. The combined detection of the two indicators is helpful for clinical diagnosis of PD.]]> 2023/12/30 8:18:06 MA Ying, LI Qin-fu, QI Li-xia 325true ［Abstract］　Objective　To analyze the diagnostic effects of polymerase chain reaction(PCR) fluorescence probe method, BD960 liquid culture method and direct smear fluorescence staining method on mycobacterium infection. Methods　From January 2021 to April 2021, 104 patients with suspected mycobacterium who visited the doctors infection in Chest Hospital of Guangxi Zhuang Autonomous Region were selected as the research subjects. The BD960 liquid culture method was used as the reference to evaluate the detection efficiency of PCR-fluorescence probe method and direct smear fluorescence staining method in detection of mycobacteria. Results　In the specimens of the 104 patients with suspected mycobacterial disease, there were significant differences in the detection results between the BD960 liquid culture method and PCR-fluorescent probe method, and between the BD960 liquid culture method and the direct smear fluorescence staining method(P=0.013, P<0.001). Conclusion　The PCR-fluorescent probe method and the BD960 liquid culture method can quickly diagnose Mycobacterium tuberculosis(MTB) and nontuberculosis mycobacteria(NTM). However, when MTB and NTM grow together, only MTB can be expressed, which has detection limitations and needs to be further improved. The detection rate of the direct smear fluorescence staining method is low, but the detection method is simple, rapid and cheap, and is suitable for preliminary identification in basic laboratories.]]> 2023/12/30 8:18:06 LEI Zhen, YANG Xiao-bing, TANG Liu-sheng 324true ［Abstract］　Objective　To compare the birth weight and placental weight percentile values of newborns with different genders and gestational weeks in Guangxi, and to compare them with the current standards and to plot the percentile curves. Methods　A total of 11 731 singleton pregnancies delivered in Maternity and Child Health Care Hospital of Guangxi Zhuang Autonomous Region during May 2019 and December 2021 were collected. The birth weight and placental weight percentile curves of the newborns with different genders and gestational weeks were plotted using generalized additive models for location, scale and shape(GAMLSS) package in R software, and their percentile values were specified and compared with the China′s current standard and the international reference standard. Results　The birth weight and placental weight percentile curves of newborns with different genders and gestational weeks in Guangxi and their percentile values were obtained, including 6 264 male newborns and 5 467 female newborns. With the increase of gestational weeks, the birth weight and placental weight of the newborns increased gradually, but the percentile values of the placental weight increased gently. Compared with those in the China current standard, the 10th percentiles(P₁₀) of the newborns′ birth weight in Guangxi were large during 31 and 38 weeks of gestation, and were small during 39 and 41 weeks of gestation. Similarly, compared with those in the China current standard, the 90th percentiles(P₉₀) of the newborns′ birth weight in Guangxi were large during 34 and 37 weeks of gestation, and were small during 38 and 41 weeks of gestation. For 33 to 41 weeks of gestation, the P₁₀ value of birth weight of the newborns in Guangxi was higher than that in the international reference standard, while the P₉₀ value of birth weight of the newborns in Guangxi was lower than that in the international reference standard. The developmental peak of placenta in male fetuses occurred earlier than that in female fetuses. Conclusion　The percentile values of the newborns′ birth weight in Guangxi are different from those in the current standards. The local standards should be adopted to plot the relevant scales to avoid diagnostic bias. In addition, the kinetics of placental development are also related to fetal genders.]]> 2023/12/30 8:18:06 WEI Hong-wei, MIAO Li-mei, QIN Yu-qin, et al. 323true ［Abstract］　Objective　To analyze the application effect of Glisson tip transected hepatectomy in paediatric liver tumour surgery. Methods　The clinical data of 18 paediatric patients with primary liver tumour who were admitted to the Department of General Surgery of Shanxi Children′s Hospital from October 2019 to October 2022 were retrospectively analyzed, and the treatment results and prognosis of the patients were summarized and analyzed. Results　The 18 paediatric patients underwent the corresponding hepatic segmentectomy or liver lobectomy successfully by using Glisson tip transected hepatectomy, and no perioperative deaths occurred. The operative time was (204.52±46.84)min, and the intraoperative blood loss was (245.73±56.86)ml, and the intraoperative blood transfusion volume was (200.84±50.57)ml. The levels of platelets(PLT) and total bile acid(TB) of the paediatric patients were decreased 1 week after surgery compared with those before surgery, and the differences were statistically significant(P<0.05). There were no significant differences in white blood cells(WBC), red blood cells(RBC), haemoglobin(Hb), alpha-fetoprotein(AFP), albumin(ALB), aspartate aminotransferase(AST), alanine aminotransferase(ALT) and lactate dehydrogenase(LDH) before and after operation(P>0.05). The postoperative serum AFP levels of 7 paediatric patients with hepatoblastoma were significantly higher than their preoperative AFP levels ［234.00(44.00, 550.00) vs 140.00(23.00, 495.00); Z=4.846, P=0.018］. By June 2023, the growth and development of 10 paediatric patients with benign liver tumors were normal, and no recurrence occurred, with a survival rate of 100%. Among the 8 paediatric patients with malignant liver tumors, 1 case with hepatoblastoma recurred 6 months after surgery and was treated with C5VD chemotherapy for 3 courses, and abdominal computed tomography(CT) examination showed a significant reduction in tumor volume. There was no recurrence in the other 7 cases. Conclusion　Glisson tip transected hepatectomy is safe and effective in the treatment of primary liver tumors in paediatric patients.]]> 2023/11/30 11:41:23 CHEN Jin-yu, XI Hong-wei 322true ［Abstract］　Objective　To study the clinical efficacy and safety of drug-eluting bead transarterial chemoembolization(DEB-TACE) combined with systemic antitumor regimen in treatment of unresectable primary hepatic carcinoma(PHC). Methods　The clinical data of 99 patients with unresectable PHC who received DEB-TACE treatment in Nanchong Central Hospital from January 2021 to December 2022 were retrospectively analyzed. According to different treatments of combined systemic anti-tumor regimen, the patients were divided into control group(receiving simple DEB-TACE treatment, 44 cases), combined group A(receiving DEB-TACE combined with targeted therapy, 24 cases), and combined group B(receiving DEB-TACE combined with immunotherapy, 16 cases) and combined group C(receiving DEB-TACE combined with immunotherapy plus targeted therapy, 15 cases). The clinical efficacy, survival prognosis and adverse reactions were compared among the four groups. Results　The objective response rate(ORR) and disease control rate(DCR) in the combined group A, the combined group B and the combined group C were higher than those in the control group, and the overall survival(OS) time and the progression-free survival(PFS) time in the combined group A, the combined group B and the combined group C were longer than those in the control group, and the differences were statistically significant(P<0.05). There were no significant differences in ORR, DCR, OS time and PFS time among the combined group A, the combined group B and the combined group C(P>0.05). Compared with the control group, the combined group A, the combined group B and the combined group C all showed the characteristic adverse reactions related to the treatment drugs. After symptomatic treatments, the symptoms related to the adverse reactions were alleviated or disappeared, and all the adverse reactions were not above grade Ⅲ. Conclusion　DEB-TACE combined with systemic anti-tumor regimen has good clinical efficacy and safety in the treatment of moderate and advanced PHC.]]> 2023/11/30 11:41:23 LIANG Guo-dong, PENG Yong, CONG Peng 321true ［Abstract］　Objective　To observe the effect of subcutaneous tissue plastic surgery combined with super tension-reduced suture in reducing postoperative scar formation in patients with thyroid nodules. Methods　Four hundred patients with thyroid nodules who underwent open thyroid surgery in Nanxishan Hospital of Guangxi Zhuang Autonomous Region from July 2020 to July 2021 were recruited and divided into control group and observation group by random number table method, with 200 cases in each group. The observation group was treated with subcutaneous tissue plastic surgery combined with super tension-reduced suture. The control group was treated with the traditional intermittent suture. The pain degrees 24 hours and 48 hours after surgery, incidence of postoperative incicison flap edema, and Vancouver Scar Scale(VSS) scar index scores 6 months and 1 year after surgery were compared between the two groups. Results　There were no surgical site infections and dehiscence of the incision in both groups, and the incision healed up to grade A. There was no significant difference in the incidence of postoperative incision flap edema(P>0.05). There were no significant differences in Visual Analogue Scale(VAS) scores between the two groups 24 hours and 48 hours after surgery(P>0.05). The VSS scar index scores of the observation group were lower than those of the control group 6 months and 1 year after surgery, and the differences were statistically significant(P<0.05). Conclusion　Subcutaneous tissue plastic surgery combined with super tension-reduced suture can effectively reduce postoperative scar formation in patients with thyroid nodules, which is worthy of clinical recommendation.]]> 2023/11/30 11:41:23 CHEN Jia-hua, ZHANG Qin-qin, ZHANG Mi, et al. 320true ［Abstract］　Objective　To study the mechanism of action of peptidylarginine deiminase 4(PAD4)-mediated citrullinated histone H3(H3cit) in sepsis-associated acute kidney injury(AKI). Methods　Thirty C57BL/6J mice were randomly divided into control group, model group and drug administration group, with 10 mice in each group. The drug administration group was intragastric administration of PAD4 inhibitor BB-Cl-amidine for 7 days, and the other two groups were given the same volume of normal saline. The AKI model was constructed by intraperitoneal injection of lipopolysaccharide(LPS) in the mice in the model group and the drug administration group 7 days later. The mice were sacrificed 24 hours later, and the levels of serum creatinine(SCr), blood urea nitrogen(BUN) and H3cit, urinary microalbumin(UALb)/creatinine(/Cr) ratio, and the levels of malondialdehyde(MDA) and superoxide dismutase(SOD) in the renal tissues were detected in each group. The correlation of the levels of SCr, BUN and UALb/Cr ratio with H3cit level was analyzed. Hematoxylin and eosin(HE) staining was used to detect the pathological status of kidney injury in mice of each group. The expression levels of PAD4 and H3cit in kidney were detected by Western blot. HEK293T cells were used for the experiment, and the blank group, LPS group and LPS+BB-Cl-amidine group were set up. The expression levels of PAD4 and H3cit protein in each group were detected by Western blot method, and the cell proliferation levels in each group were detected by CCK-8 method. Results　In the animal experiments, compared with those in the control group, the levels of serum SCr, BUN and H3cit in the model group were increased, and the urinary UALb/Cr ratio was increased, and the MDA level in the renal tissues was increased, and the SOD level in the renal tissues was decreased, and the renal pathological injury was aggravated, and the expressions of PAD4 and H3cit protein were increased, and the differences were statistically significant(P<0.05). Compared with those in the mice of the model group, the levels of serum SCr, BUN and H3cit were decreased, and the urinary UALb/Cr ratio was decreased, and the MDA level in the renal tissues was decreased, and the SOD level in the renal tissues was increased, and the renal pathological injury was relieved, and the expression of H3cit protein was decreased in the mice of the drug administration group, and the differences were statistically significant(P<0.05). The level of H3cit was positively correlated with the levels of SCr, BUN and UALb/Cr ratio(P<0.05). In the cell experiment, compared with those in the blank group, the expressions of PAD4 and H3cit protein in the LPS group were increased, and the level of cell proliferation in the LPS group was decreased, and the differences were statistically significant(P<0.05). Compared with those in the LPS group, the expression of H3cit protein was decreased and the level of cell proliferation was increased in the LPS+BB-Cl-amidine group, and the differences were statistically significant(P<0.05). Conclusion　PAD4-mediated H3cit can promote the development of sepsis-associated AKI, and inhibition of PAD4 activity can effectively reduce kidney cell injury.]]> 2023/11/30 11:41:23 WEI Ze-feng, WANG Zi-qiang, ZHENG Jin-hua, et al. 319true ［Abstract］　Objective　To analyze the effects of oral appliances on the treatment of different severities of obstructive sleep apnea(OSA). Methods　Seventy-four patients with OSA or primary snoring who were admitted to the Department of Orthodontics of the People′s Hospital of Guangxi Zhuang Autonomous Region from December 2009 to October 2019 were selected. According to the results of polysomnography(PSG), the patients were divided into mild OSA group(18 cases), moderate OSA group(18 cases), severe OSA group(21 cases) and primary snoring group(17 cases). All the four groups were treated with mandibular advancement oral appliances. The changes of sleep indexes and respiratory indexes before and after treatment were compared among the four groups. Results　Compared with those before treatment, the proportion of stage N3 in the mild OSA group was increased, and the arousal index(ArI) in the moderate OSA group was decreased, and the proportion of stage N1 in the severe OSA group was decreased, and the differences were statistically significant(P<0.05). Compared with those before treatment, the apnea index(AI), hypopnea index(HI), apnea hypopnea index(AHI), the longest duration of apnea and the longest duration of hypopnea decreased and the lowest arterial oxygen saturation(LSpO₂) during sleep increased in the mild OSA group. The AI, HI, AHI and the longest apnea time decreased and LSpO₂ during sleep increased in the moderate OSA group. The AI, HI, AHI and the longest apnea time and the longest duration of hypopnea decreased in the severe OSA group, while the LSpO₂ during sleep and average arterial oxygen saturation(ASpO₂) in awake state in the severe OSA group increased, and the differences were statistically significant(P<0.05). Conclusion　Oral appliance treatment can promote sleep improvement in the patients with mild and moderate OSA. After treatment, the respiratory parameters of the patients with different severities of OSA are improved.]]> 2023/11/30 11:41:23 RAO Ming-cong, ZHONG Rong-bing, ZHANG Qiu-ping, et al. 318true ［Abstract］　Objective　To compare the therapeutic effects between simple decompression and decompression internal fixation fusion for recurrent lumbar disc herniation(RLDH). Methods　The clinical data of 73 patients with RLDH who were admitted to the Sixth Affiliated Hospital of Xinjiang Medical University from October 2017 to August 2021 were selected, and the patients were divided into simple decompression group(31 cases) and decompression internal fixation fusion group(42 cases) according to the surgical procedures they received. The operation time, intraoperative blood loss, bed rest time, incidence of cerebrospinal fluid leakage, and Visual Analogue Scale(VAS) scores and Oswestry disability index(ODI) scores before surgery and 3 days, 3 months, 6 months and 1 year after surgery were compared between the two groups. Results　The operation time and the bed rest time in the simple decompression group were shorter than those in the decompression internal fixation fusion group, and the intraoperative blood loss in the simple decompression group was less than that in the decompression internal fixation fusion group, and the differences were statistically significant(P<0.05). There was no statistically significant difference in the incidence of cerebrospinal fluid leakage between the two groups(P>0.05). There was no significant difference in clinical effective rate between the two groups(74.19% vs 71.43%; χ²=0.069, P=0.793). The VAS scores and ODI scores showed a downward trend in the two groups after surgery, and the differences were statistically significant compared with those in the same group before surgery(P<0.05). However, the differences in the VAS scores and ODI scores were not statistically significant between the two groups at each time point(P>0.05). Conclusion　Both simple decompression and decompression internal fixation fusion can effectively treat RLDH. Simple decompression has more advantages in shortening the operation time and bed rest time, and in reducing the intraoperative blood loss.]]> 2023/11/30 11:41:23 HU Yong-sheng, ZHANG Xiao-xuan, SONG Xing-hua, et al. 317true ［Abstract］　Objective　To analyze the clinical characteristics and risk factors of the gout patients complicated with bone erosion. Methods　The clinical data of 590 gout patients who were diagnosed and treated in the Department of Rheumatology and Immunology of the Affiliated Yantai Yuhuangding Hospital of Qingdao University from January 2022 to February 2023 were retrospectively analyzed. According to the results of ultrasonography, the patients were divided into bone erosion group(223 cases) and non-bone erosion group(367 cases). The clinical characteristics of the gout patients complicated with bone erosion were analyzed. Multivariate logistic regression was used to analyze the risk factors of the gout patients complicated with bone erosion, and the predictive efficacy of the studying indicators in the gout patients complicated with bone erosion was analyzed using receiver operating characteristic(ROC) curve. Results　Among the 590 gout patients, 223 cases(37.80%) were complicated with bone erosion, with the first metatarsophalangeal joint being the most common predilection site(74.44%), and the single joint was the most involved site(65.92%). The results of multivariate logistic regression analysis showed that long course of the disease, high frequency of attack and high level of cystatin C(CysC) were the independent risk factors for promoting the occurrence of gout complicated with bone erosion(P<0.05). The results of ROC curve analysis showed that disease duration, attack frequency and CysC level could effectively predict the occurrence of gout complicated with bone erosion(P<0.05), and the combination of these three indicators could further improve the prediction efficiency［AUC(95%CI)=0.933(0.911-0.955), P<0.001］, and the sensitivity and specificity were 87.00% and 87.74%, respectively. Conclusion　The first metatarsophalangeal joint is the mainly involved site in the gout patients complicated with bone erosion. For the patients with prolonged disease course, frequent attacks and increased CysC level, active intervention should be given to prevent disease progression.]]> 2023/11/30 11:41:23 ZHANG Fu-yun, ZHAO Na, TANG Yan-chun 316true ［Abstract］　Objective　To compare the therapeutic effects between transdeltoid approach and deltoid-pectoralis major space approach in treatment of proximal humerus fractures in elderly patients. Methods　Fifty elderly patients with proximal humerus fractures who underwent open reduction and internal fixation surgery in the Fifth People′s Hospital of Qinghai Province from February 2021 to January 2023 were recruited. The control group included 25 patients who underwent deltoid-pectoralis major space approach. The observation group included 25 patients who underwent transdeltoid approach. The operation time, intraoperative blood loss, duration of hospital stay, fracture healing time, incision healing time, postoperative complications, postoperative Constant-Murley scores and Visual Analogue Scale(VAS) scores of shoulder joints were compared between the two groups. Results　The operation time and the incision healing time in the observation group were shorter than those in the control group, and the intraoperative blood loss in the observation group was less than that in the control group, and the differences were statistically significant(P<0.05). There were no significant differences in the fracture healing time and the duration of hospital stay between the two groups(P>0.05). The VAS scores in the observation group were lower than those in the control group immediately after operation and 7 days after operation, and the Constant-Murley scores of the shoulder joints in the observation group were higher than those in the control group, and the differences were statistically significant(P<0.05). There were no significant differences in the VAS scores and Constant-Murley scores of the shoulder joints 3 months after operation between the two groups(P>0.05). There was no significant difference in the incidence of complications between the two groups(12.00% vs 16.00%; χ²=0.000, P=1.000). Conclusion　The treatment of proximal humerus fracture by transdeltoid approach can reduce intraoperative blood loss, relieve wound pain, shorten incision healing time, facilitate the early implementation of functional exercise after operation, and improve shoulder joint function.]]> 2023/11/30 11:41:23 ZHAO Huan-rong, MA Zhi-hua, MA Jun-jun, et al. 315true ［Abstract］　Objective　To observe the application effect of neuromuscular electrical stimulation combined with programmed lower limb rehabilitation training in elderly patients with intertrochanteric fracture of femur. Methods　Eighty-six elderly patients with intertrochanteric fracture of femur who received surgical treatment in Beijing Rehabilitation Hospital, Capital Medical University from January 2019 to September 2021 were recruited and divided into control group and observation group by random number table method, with 43 cases in each group. The control group received routine rehabilitation treatment, while the observation group received additional neuromuscular electrical stimulation combined with programmed lower limb rehabilitation training on the basis of routine rehabilitation treatment. Fracture healing, complications, bone metabolic markers, motor function recovery and quality of life were compared between the two groups. Results　The fracture healing time, time getting out of bed, partial weight-bearing time and full weight-bearing time in the observation group were shorter than those in the control group, and the differences were statistically significant(P<0.05). The incidence of postoperative complications in the observation group was significantly lower than that in the control group(4.65% vs 18.60%; χ²=4.074, P=0.044). At 1 week, 3 months and 6 months after surgery, the Visual Analogue Scale(VAS) scores in the observation group were lower than those in the control group, and the Harris scores and Barthel index(BI) scores in the observation group were higher than those in the control group, and the differences were statistically significant(P<0.05). At 3 and 6 months after surgery, the levels of osteocalcin(OC) and amino-terminal propeptide of type Ⅰ precollagen(PINP) in the observation group were higher than those in the control group, and the level of C-terminal telopeptide of type Ⅰ collagen(CTX) in the observation group was lower than that in the control group, and the differences were statistically significant(P<0.05). Conclusion　Neuromuscular electrical stimulation combined with programmed lower limb rehabilitation training can help to promote fracture healing and motor function recovery in elderly patients with intertrochanteric fracture of femur after surgery, reduce complications and improve quality of life. The mechanisms of these actions may be related to the regulation of bone metabolic markers.]]> 2023/11/3 19:25:14 LI Wei, WANG Ye, LI Nan, et al. 314true ［Abstract］　Objective　To investigate the relationship between serum soluble CXC chemokine ligand 16(sCXCL16) level and the progression and prognosis of diabetic kidney disease(DKD) in elderly patients with type 2 diabetes mellitus(T2DM). Methods　Fifty-five patients with DKD(DKD group) and 54 patients with simple T2DM(T2DM group) who were admitted to Chuiyangliu Hospital Affiliated to Tsinghua University from April 2017 to January 2020 were recruited as the study subjects, and 55 healthy subjects undergoing physical examination were selected as the control group. Serum sCXCL16 level was compared among the three groups, and the relationship of the serum sCXCL16 level with DKD progression and prognosis was analyzed. Results　The serum sCXCL16 level in the DKD group and the T2DM group was higher than that in the control group, and the serum sCXCL16 level in the DKD group was higher than that in the T2DM group, and the differences were statistically significant(P<0.05). For the DKD patients, the serum sCXCL16 level in the macroalbuminuria subgroup was higher than that in the microalbuminuria subgroup(P<0.05). The results of Spearman rank correlation analysis showed that serum sCXCL16 level was positively correlated blood urea nitrogen(BUN), creatinine(Cr) and urinary albumin excretion(UAE), homeostasis model assessment of insulin resistance(HOMA-IR), 24-hour proteinuria, tumor necrosis factor-α(TNF-α) and hypersensitive C-reactive protein(hs-CRP)(P<0.05). The results of multivariate logistic regression analysis showed that serum sCXCL16>18.50 ng/ml was an independent risk factor for DKD in elderly T2DM patients(P<0.05). The results of receiver operating characteristic(ROC) curve analysis showed that serum sCXCL16 level could effectively differentiate T2DM from DKD［ AUC(95%CI)=0.866(0.801-0.931), P<0.001］, and could effectively predict end-stage renal disease(ESRD) in the DKD patients［ AUC(95%CI)=0.854(0.747-0.962), P<0.001］. The results of multivariate Cox regression analysis showed that serum sCXCL16 level ≥20.02 ng/ml was an independent risk factor for ESRD in the DKD patients(P<0.05). Conclusion　Elevated serum sCXCL16 level in elderly T2DM patients is closely related to the occurrence and development of DKD, and the monitoring of serum sCXCL16 level is helpful for clinicians to evaluate the patients′ condition.]]> 2023/11/3 19:25:14 ZENG Zhen, PENG Fu-song, WANG Hong-jiang 313true ［Abstract］　Objective　To observe the clinical effect of anterior cervical sternocleidomastoid approach on treatment of unilateral thyroid carcinoma. Methods　The clinical data of 97 patients who underwent surgical treatment of unilateral thyroid carcinoma in the Affiliated Huai′an No.1 People′s Hospital of Nanjing Medical University from August 2022 to December 2022 were selected and divided into three groups according to different surgical approaches: the cervical group(24 cases, receiving anterior cervical sternocleidomastoid approach), the median group(50 cases, receiving traditional median incision approach) and the endoscopic group(23 cases, receiving non-inflatable axillary approach endoscopy). The perioperative related indicators, postoperative cosmetic effects and complications were compared among the three groups. Results　There were no significant differences in the number of lymph nodes dissected and the number of lymph node metastases among the three groups(P>0.05). There were no significant differences in the operation time, intraoperative blood loss and postoperative drainage volume between the cervical group and the median group(P>0.05). The length of hospital stay in the cervical group was significantly shorter than that in the endoscopic group(P<0.05). Compared with the cervical group and the median group, the endoscopic group had longer operation time and retention time of the drainage tube, and larger intraoperative blood loss and postoperative drainage volume, and the differences were significant(P<0.05). The Visual Analogue Scale(VAS) scores in the endoscopic group were higher than those in the median group and the cervical group 1 day after surgery, and the differences were significant(P<0.05). There were no significant differences in the Non-Surgical Symposium(NSS) scores between the endoscopic group and the cervical group(P>0.05), and their NSS scores were significantly higher than those in the median group 3 months after surgery(P<0.05). The total postoperative complication rate in the cervical group was significantly lower than that in the median group(8.33% vs 36.00%, P<0.05), but there was no significant difference between the cervical group and the endoscopic group(8.33% vs 21.74%, P>0.05). Conclusion　Compared with the traditional median incision approach and the non-inflatable axillary approach endoscopy, the anterior cervical sternocleidomastoid approach can ensure the safety and effectiveness for the patients in the treatment of unilateral thyroid carcinoma, while also taking into account the aesthetic effect of the incision, reducing postoperative complications for the patients, and is worthy of clinical recommendation.]]> 2023/11/3 19:25:14 TANG Jie, LI Zhi, WEI Wan, et al. 312true ［Abstract］　Objective　To explore the analgesic mechanism of Zhuang Medicine meridian massage in neuropathic pain(NPP) rats. Methods　Forty-five Sprague-Dawley(SD) rats were randomly divided into model group, normal group, Zhuang Medicine meridian massage group, sham massage group, and sham operation group, with 9 rats in each group. An L₅ spinal nerve ligation(SNL) model was established in the other three groups except the normal group and the sham operation group. Interventions were started on the first day after operation in the sham massage group(gently touching the rats′ hind limbs) and the Zhuang Medicine meridian massage group for 14 consecutive days, while the other three groups did not receive massage or touching intervention. The paw withdrawal mechanical thresholds(PWMT) and the paw withdrawal thermal latency(PWTL) were measured in the rats in each group before operation and 1, 3 and 14 days after operation. On the 14th day after operation, the expression of caspase-1 in the dorsal horn of the L_4-6 spinal cord in the rats was detected by real-time quantitative polymerase chain reaction(RT-qPCR), and the expression of interleukin-18(IL-18) in the serum was detected by enzyme-linked immunosorbent assay(ELISA), and the expression of lactate dehydrogenase(LDH) in the serum was detected by microplate method. Results　There were no significant differences in PWMT and PWTL among the five groups before operation(P>0.05). Compared with those in the normal group, the PWMT and PWTL in the other four groups were significantly decreased on the 1st day and the 3rd day after operation(P<0.05). On the 14th day after operation, the PWMT and PWTL in the Zhuang Medicine meridian massage group were higher than those in the model group and the sham massage group, and the differences were significant(P<0.05). On the 14th day after operation, the expression levels of caspase-1 in the spinal dorsal horn tissues and the serum IL-18 and LDH in the model group were significantly higher than those in the normal group(P<0.05). Compared with those in the model group and the sham massage group, the expression levels of caspase-1 in the spinal cord tissues and the serum IL-18 and LDH in the Zhuang Medicine meridian massage group were lower, and the differences were significant(P<0.05). Conclusion　Zhuang Medicine meridian massage can alleviate pain sensitivity in the SNL model rats, and its mechanism is related to down-regulating the expression of caspase-1 in the dorsal horn of the spinal cord and inhibiting the immune inflammatory reaction mediated by pyroptosis.]]> 2023/11/3 19:25:14 LIANG Ying-ye, WANG Tian-yi, LU Dong-ming, et al. 311true ［Abstract］　Objective　To explore the effects of micro ribonucleic acid-630(miR-630) on the occurrence and development of clear cell renal cell carcinoma(ccRCC) and their mechanisms. Methods　Eighty-six patients with ccRCC who were admitted to Affiliated Hospital of Hebei University of Engineering from July 2020 to July 2021 were selected. The tumor tissues and the adjacent normal tissues(more than 5 cm away from the tumor focus) were taken during the operation, and the expression levels of miR-630 in the tumor tissues and the adjacent normal tissues were detected by using real-time quantitative polymerase chain reaction(RT-qPCR). The correlations of the expression levels of miR-630 in the tumor tissues and the adjacent normal tissues with the clinicopathological features of the ccRCC patients were analyzed. The correlation between the expression level of miR-630 and the expression level of apoptosis marker protein in the ccRCC tissues was analyzed. The 786-O cells were selected for experiment, and miR-630 NC group, miR-630 mimic group and miR-630 inhibitor group(They were the control, overexpression and inhibition groups of miR-630, respectively) were designed. The effects of miR-630 expression on cell proliferation activity, apoptosis rate and the expression level of apoptosis marker protein were analyzed. Results　The level of miR-630 in the ccRCC tissues was significantly higher than that in the adjacent normal tissues(P<0.05). High levels of miR-630 were associated with larger tumor diameter, high clinical stage and lymphatic metastasis(P<0.05). Compared with those in the adjacent normal tissues, the expression levels of caspase-3 protein in the ccRCC tissues were significantly decreased(P<0.05), and the levels of B cell lymphoma leukemia-2(Bcl2) protein in the ccRCC tissues were significantly increased(P<0.05). The expression level of miR-630 in the ccRCC tissues was negatively correlated with the expression level of caspase-3 protein(r=-0.489, P=0.011), and positively correlated with the expression level of Bcl2 protein(r=0.516, P=0.004). The overexpression of miR-630 could significantly promoted cell proliferation activity, inhibited cell apoptosis rate and the expression level of caspase-3 protein, and promoted the expression level of Bcl2 protein(P<0.05). Reducing the expression of miR-630 could significantly inhibit cell proliferation activity, promoted cell apoptosis and the expression level of caspase-3 protein, and inhibited the expression level of Bcl2 protein(P<0.05). Conclusion　The level of miR-630 is up-regulated in ccRCC tissues, and high level of miR-630 can inhibit cell apoptosis, and is associated with high clinical stage and lymphatic metastasis in ccRCC patients.]]> 2023/11/3 19:25:14 TAN Chao, WANG Yang, SU Jian-nan, et al. 310true ［Abstract］　Objective　To analyze the correlation of cell classification and inflammatory factor levels in bronchoalveolar lavage fluid(BALF) with the prognosis of pediatric patients suffering from Mycoplasma pneumoniae pneumonia(MPP) complicated with airway mucus plugs. Methods　The clinical data of 168 pediatric patients with MPP who received bronchoalveolar lavage in Jiamusi Central Hospital from February 2018 to February 2022 were selected. According to whether the presence of airway mucus plugs was detected under bronchoscopy, the pediatric patients were divided into MPP complicated with airway mucus plug group(78 cases) and simple MPP group(90 cases). According to the results of imaging reexamination after 2 months of disease course, the patients in the MPP complicated with airway mucus plug group were divided into slowly resolving pneumonia group(35 cases) and completely resolving pneumonia group(43 cases). The level of cell classification in BALF was determined by Rayleigh Giemsa staining method. Enzyme-linked immunosorbent assay was used to detect the levels of interleukin(IL)-2, IL-4, IL-8, and IL-10 in BALF. Multivariate logistic regression was used to analyze the influencing factors of slowly resolving pneumonia in the pediatric patients suffering from MPP complicated with airway mucus plugs. Receiver operating characteristic(ROC) curve was used to analyze the predictive value of each index for the pediatric patients suffering from MPP complicated with airway mucus plugs. Results　The percentage of macrophages, IL-4 and IL-10 levels in BALF in the MPP complicated with airway mucus plug group were lower than those in the simple MPP group, while the percentage of neutrophils, IL-2 and IL-8 levels in BALF in the MPP complicated with airway mucus plug group were higher than those in the simple MPP group, and the differences were significant(P<0.05). The results of multivariate logistic regression analysis showed that longer duration of fever and higher percentage of neutrophils in BALF were independent risk factors for slowly resolving pneumonia in the pediatric patients suffering from MPP complicated with airway mucus plugs(P<0.05), and higher percentage of macrophages in BALF was a protective factor against slowly resolving pneumonia(P<0.05). The results of ROC curve analysis showed that the combination of the duration of fever, percentage of macrophages in BALF and percentage of neutrophils in BALF could effectively predict the occurrence of slowly resolving pneumonia in the pediatric patients suffering from MPP complicated with airway mucus plugs(P<0.05), and the sensitivity and specificity were 89.00% and 75.00%, respectively. Conclusion　The combination of the duration of fever, percentage of macrophages in BALF and percentage of neutrophils in BALF can effectively predict the prognosis of the pediatric patients suffering from MPP complicated with airway mucus plugs and guide clinical treatments for them.]]> 2023/11/3 19:25:14 YANG Li-wei, QIU You-xi, YANG Song 309true ［Abstract］　Objective　To investigate the risk factors for central venous catheter-related thrombosis(CRT) in malignant tumor patients and to construct a risk prediction model. Methods　The clinical data of 2 096 patients with malignant tumors who received central venous catheter(CVC) catheterization at the Third Affiliated Hospital of Kunming Medical University from January 2019 to June 2022 were selected. The patients were divided into CRT group(with CRT formation, n=178) and control group(without CRT formation, n=1 918) according to their different CRT formations. Multivariate logistic regression was used to analyze the risk factors affecting CRT formation, and a nomogram model was established. Receiver operating characteristic(ROC) curve and calibration graph were used to analyze the predictive efficiency of the model. Results　The incidence of CRT in the 2 096 cases in this study was 8.50%(178/2 096). The results of multivariate logistic regression analysis showed that TNM stage Ⅱ, stage Ⅲ and stage Ⅳ were the risk factors for promoting CRT formation if TNM stage Ⅰ was used as a reference(P<0.05). Complicated infections, hyperlipidemia, history of thrombosis/hypercoagulability, use of hormones, and higher D-dimer levels were risk factors for CRT formation(P<0.05). The results of ROC curve analysis showed that the constructed nomogram model had good prediction efficiency［AUC(95%CI)=0.856(0.824-0.889), P<0.001］. The results of internal verification of Bootstrap method showed that the concordance index(C-index) was 0.824. The results of calibration diagram and clinical decision curve indicated that the nomogram model had better clinical application value. Conclusion　TNM stage, complicated infection, use of hormones, hyperlipidemia, history of thrombosis/hypercoagulability, and D-dimer level are independent influencing factors for CRT after CVC catheterization in patients with malignant tumors. The nomogram model constructed in this study has better prediction efficiency and has certain clinical guiding value for prevention and treatment of CRT.]]> 2023/11/3 19:25:14 WANG Xue-xing, HE Yuan, CHU Jie, et al. 308true ［Abstract］　Objective　To analyze the application value of serum testosterone, estradiol(E₂) and testosterone/E₂ ratio in evaluation of spermatogenic dysfunction. Methods　The clinical data of 750 male patients who visited the Reproductive Center of Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region from June 2020 to December 2021 were selected. Among the 750 male patients, semen quality was normal in 300 cases(normal control group), and spermatogenic dysfunction in 450 cases. According to the total number of sperm, the spermatogenic dysfunction patients were divided into mild oligospermia group, severe oligospermia group and non-obstructive azoospermia group, with 150 cases in each group. The percentile distribution of sex hormone index levels in the normal semen patients was analyzed. Serum follicle stimulating hormone(FSH), total testosterone(TT), E₂, free testosterone(FT), TT/E₂ ratio and FT/E₂ ratio were compared among the four groups. Results　The mean values of TT and FT in the male patients with normal semen quality were 504.50 ng/dl and 10.28 ng/dl, respectively, and the 5th percentile values were 273.28 ng/dl and 6.70 ng/dl, respectively. The mean values of TT/E₂ and FT/E₂ ratios in the male patients with normal semen quality were 15.75 and 0.32, respectively, and the 20th percentile values were 10.06 and 0.23, respectively. As the severity of spermatogenic dysfunction worsens, the levels of FSH showed an upward trend while the levels of E₂, TT and FT showed a downward trend. There were significant differences in the levels of TT, FT and E₂, TT/E₂ ratio and FT/E₂ ratio between the non-obstructive azoospermia group and the normal control group(P<0.05). The level of FSH in the non-obstructive azoospermia group was significantly higher than that in the mild oligospermia group and the severe oligospermia group(P<0.05). The level of FT and FT/E₂ ratio in the non-obstructive azoospermia group were significantly lower than those in the mild oligospermia group and the severe oligospermia group(P<0.05). Compared with the normal control group and the mild oligospermia group, the level of FSH in the severe oligospermia group was increased, while the levels of TT and FT, TT/E₂ ratio and FT/E₂ ratio in the severe oligospermia group were decreased, and the differences were statistically significant(P<0.05). The level of FSH in the mild oligospermia group was higher than that in the normal control group, and the level of FT in the mild oligospermia group was lower than that in the normal control group, and the differences were statistically significant(P<0.05). Conclusion　In the evaluation of spermatogenic dysfunction, serum FT is an important indicator to be concerned about, and the lower limits of TT and FT reference values are 273.28 ng/dl and 6.70 ng/dl, respectively. The TT/E₂ ratio <10.06 can be used as the therapeutic threshold value.]]> 2023/11/3 19:25:14 GAN Xian-you, XU Kong-rong, LING Xiao-bin, et al. 307true ［Abstract］　Objective　To explore the effect of dental pulp stem cells-conditioned medium(DPSCs-CM) combined with layered double hydroxide(LDH) nanomaterials on photoaging HaCaT cells. Methods　DPSCs-CM was prepared and LDH nanomaterials were synthesized. The control group［no pretreatment, no exposure to ultraviolet B(UVB)］, model group(no pretreatment, exposure to UVB), DPSCs-CM group(undergoing UVB irradiation after 24-hour pretreatment with DMEM complete medium containing DPSCs-CM), LDH group(undergoing UVB irradiation after 24-hour pretreatment with DMEM complete medium containing LDH) and DPSCs-CM+LDH group(undergoing UVB irradiation after 24-hour pretreatment with DMEM complete medium containing DPSCs-CM and LDH mixed in equal proportion) were set up. The content of malondialdehyde(MDA), the activity of superoxide dismutase(SOD) and the level of reactive oxygen species(ROS) of HaCaT cells were detected in each group. The migration ability of HaCaT cells in each group was detected by using scratch wound healing assay. The expression levels of p53 protein and p16 protein of HaCaT cells in each group were detected by using Western blot method. Results　The average particle size of the LDH nanomaterials was 65.52 nm, with a polydispersity index(PDI) of 0.109 and a Zeta potential of 38.1 mV, indicating that LDH nanomaterials had low cytotoxicity, uniform particle size distribution, and good dispersion and stability. Compared with the model group, the DPSCs-CM group, LDH group and DPSCs-CM+LDH group had the low level of ROS and low content of MDA, high activity of SOD, the enhanced migration ability of HaCaT cells and reduced expression levels of intracellular p53 protein and p16 protein, and the differences were statistically significant(P<0.05). The intervention effect of the DPSCs-CM+LDH group was the best. Conclusion　DPSCs-CM combined with LDH nanomaterials can effectively prevent photoaging of HaCaT cells, and its effect is better than that of single DPSCs-CM intervention or LDH nanomaterials intervention, which provides a reference for the research and development of skin photoaging treatment methods.]]> 2024/9/29 12:29:45 QIU Liuliu, XIE Shenxia, YANG Xiaomei, LU Xiaoling 306true ［Abstract］　Objective　To compare the application effects of remimazolam and propofol combined with sufentanil in elderly patients undergoing painless gastroscopy and their impacts on recovery quality. Methods　A total of 134 elderly patients undergoing painless gastroscopy in the Affiliated Hospital of Guizhou Medical University from January 2022 to June 2022 were recruited and divided into control group and observation group by random number table method, with 67 cases in each group. The anesthesia regimen of propofol combined with sufentanil was used in the control group, and the anesthesia regimen of remimazolam combined with sufentanil was used in the observation group. The onset time of anesthesia, time of operating gastroscope, awakening time, Quality of Recovery-15(QoR-15) scale scores on the first day after examination, as well as the heart rate(HR), respiratory rate(RR), pulse oxygen saturation(SpO₂) and mean arterial pressure(MAP) at the time of entry into the operating room(T₁), at the start time of sufentanil administration(T₂), at the start time of remimazolam/propofol administration(T₃),at the time when the insertion tube of the gastroscope was being inserted(T₄), at the time of 3 minutes after the insertion tube of the gastroscope was inserted(T₅), at the time of 5 minutes after the insertion tube of the gastroscope was inserted(T₆), and at the time when the insertion tube of the gastroscope was withdrawn(T₇) between the two groups. The occurrence of adverse reactions was compared between the two groups. Results　On the first day after examination, the total QoR-15 scale scores in the observation group were significantly higher than those in the control group(P<0.05), and among the five dimensions of the QoR-15 scale, the pain scores, physical comfort scores and physical independence scores in the observation group were higher than those in the control group, and the mood state scores in the observation group were lower than those in the control group, and the differences were statistically significant(P<0.05). There were no significant differences in psychological support scores between the two groups(P>0.05). From the time point of T₄ to the time point of T₇, the MAP levels in the observation group were significantly higher than those in the control group(P<0.05). From the time point of T₄ to the time point of T₆, the SpO₂ levels in the observation group were significantly higher than those in the control group(P<0.05). There were no significant differences in HR and RR levels between the two groups at each observation time point(P>0.05). There was no significant difference in the time of operating gastroscope and the onset time of anesthesia between the two groups(P>0.05). The observation group woke up faster than the control group, and the difference was statistically significant(P<0.05). The proportion of the patients using vasoactive drugs and the proportion of the patients with drug addition times more than 1, and the incidence rates of hypotension, respiratory depression, nausea and vomiting, choking cough, dizziness, delirium and injection pain in the observation group were lower than those in the control group, and the differences were statistically significant(P<0.05). Conclusion　The application of remimazolam combined with sufentanil shows good effects on the elderly patients undergoing painless gastroscopy, with stable hemodynamics and low incidence of adverse reactions during the examination, which helps to improve the patients′ recovery quality.]]> 2024/9/29 12:29:45 1, CHEN Yuanyang¹, WU Hongqi¹, JIANG Ke^1,2, ZOU Xiaohua^1,2]]> XU Hechen¹, CHEN Yuanyang¹, WU Hongqi¹, JIANG Ke^1,2, ZOU Xiaohua^1,2 305true ［Abstract］　Objective　To explore the characteristics of attention bias in Wilson′s disease(WD) patients with depressive tendencies and its relationship with rumination. Methods　A total of 51 WD patients admitted to Affiliated Hospital of Institute of Neurology, Anhui University of Chinese Medicine from January 2022 to January 2023 were recruited. According to Beck Depression Inventory(BDI) scores, the patients were divided into depression group(≥5 points, 30 cases) and non-depression group(≤4 points, 21 cases). The results of Ruminative Responses Scale(RRS) scores and emotional Stroop paradigm were compared between the two groups, and the correlation between the observation indicators was analyzed. Results　The dimension scores of rumination symptoms, introspection and forced thinking in RRS and the total scores of RRS in the depression group were higher than those in the non-depression group, and the differences were statistically significant(P<0.05). The response time to negative words in the depression group was significantly longer than that in the non-depression group(P<0.05). There was no significant difference in the response time to neutral words and positive words between the two groups(P>0.05). In the WD patients, the response time to negative words was positively correlated with BDI scores, total RRS scores and the dimension scores of rumination symptoms in RRS(P<0.05), and there was no significant correlation of the response time to positive words and the response time to neutral words with BDI scores, total RRS scores and each dimension scores of RRS(P>0.05). The results of multi-factor linear regression analysis showed that BDI scores, total RRS scores and the dimension scores of rumination symptoms in RRS were the influencing factors of the response time of the WD patients to negative words(P<0.05). Conclusion　The WD patients with depressive tendencies tend to pay too much attention to negative emotional information. Rumination may be one of the causes of negative emotional attention bias in the WD patients with depressive tendencies.]]> 2024/9/29 0:00:00 1,2, BAI Xue^1,2, WANG Gongqiang^2,3, MA Xinfeng², LIN Kang², JIN Ping², SHI Beibei^1,2, HAN Yongzhu^2,3]]> WEN Xiao^1,2, BAI Xue^1,2, WANG Gongqiang^2,3, MA Xinfeng², LIN Kang², JIN Ping², SHI Beibei^1,2, HAN Yongzhu^2,3 304true ［Abstract］　Objective　To explore the effects of iron overload on T lymphocyte subsets and survival prognosis in patients with newly diagnosed myelodysplastic syndrome(MDS). Methods　The clinical data of 58 patients with MDS who were admitted to Yuncheng Central Hospital Affiliated to Shanxi Medical University from January 2018 to December 2023 were retrospectively analyzed, and the study subjects were divided into iron overload group［serum ferritin(SF)≥1 000 ng/mL, 27 cases］ and non-iron overload group(SF<1 000 ng/mL, 31 cases) according to their SF levels. The general clinical data, lymphocyte subsets and survival prognosis were compared between the two groups. Results　The levels of SF and transferrin saturation in the iron overload group were higher than those in the non-iron overload group, and the levels of peripheral blood CD3⁺ T lymphocytes, CD4⁺ T lymphocytes and CD8⁺ T lymphocytes in the iron overload group were lower than those in the non-iron overload group, and the differences were statistically significant(P<0.05). The median survival time was 8 months in the iron overload group and 30 months in the non-iron overload group. The overall survival time of the non-iron overload group was significantly better than that of the iron overload group(log-rank test: χ²=5.368, P=0.020). The results of multivariate Cox regression analysis showed that iron overload［HR(95%CI)=3.50(1.46-8.39), P=0.005］ and the Revised International Prognostic Scoring System(IPSS-R) risk classification at very high risk［HR(95%CI)=6.28(1.15-34.27), P=0.034］ were independent risk factors affecting the survival prognosis of the patients with MDS(P<0.05). Conclusion　Iron overload is a risk factor for poor survival prognosis in MDS patients, and iron overload can affect the level of T lymphocyte subsets, which deserves the attention of clinicians.]]> 2024/9/29 12:29:45 1, YU Wenjun², LI Liang³, JIAO Xiaolin¹, ZHAO Dan¹, MA Yaofang¹, TAO Yongming³]]> DUAN Lixiang¹, YU Wenjun², LI Liang³, JIAO Xiaolin¹, ZHAO Dan¹, MA Yaofang¹, TAO Yongming³ 303true ［Abstract］　Objective　To explore the effects of active circulatory breathing technique combined with humidified high-flow oxygen therapy on respiratory muscle function and pulmonary aspiration in stroke patients undergoing tracheotomy. Methods　A total of 70 stroke patients receiving tracheotomy in Cangzhou People′s Hospital from May 2022 to May 2023 were recruited and divided into observation group and control group by random number table method, with 35 cases in each group. The control group received humidified high-flow oxygen therapy, while the observation group received active circulatory breathing technique combined with humidified high-flow oxygen therapy. The sputum viscosity, respiratory muscle function, lung function, Penetration-Aspiration Scale(PAS) scores and Functional Independence Measurement(FIM) scores were compared between the two groups. Results　After the treatment of 2 weeks, the sputum viscosity was significantly improved compared with that before treatment in both groups(P<0.05), and the proportion of the patients with sputum viscosity of Ⅰ-Ⅱ degrees in the observation group was higher than that in the control group, and the differences were statistically significant(P<0.05). After treatment, the levels of maximum inspiratory pressure(MIP), maximum expiratory pressure(MEP), forced expiratory volume in 1 second(FEV₁), forced vital capacity(FVC) and FEV₁/FVC in both groups were significantly increased compared with those before treatment(P<0.05), and the levels of MIP, MEP, FEV₁, FVC and FEV₁/FVC in the observation group were higher than those in the control group, and the differences were statistically significant(P<0.05). Compared with those in both groups before treatment, the PAS scores and the incidence of pulmonary aspiration in both groups after treatment were significantly decreased(P<0.05), and the FIM scores in both groups after treatment were significantly increased(P<0.05). After treatment, the PAS scores and the incidence of pulmonary aspiration in the observation group were lower than those in the control group, and the FIM scores in the observation group were higher than those in the control group, and the differences were statistically significant(P<0.05). During the treatment period, no adverse events related to the treatment measures were observed in both groups. Conclusion　Active circulatory breathing technique combined with humidified high-flow oxygen therapy can improve respiratory muscle function and lung function, reduce sputum viscosity and the incidence of pulmonary aspiration, and improve the patients′ quality of life.]]> 2024/9/29 12:29:45 1, JIN Liang², DAI Yan³, QI Xiaoru⁴, YU Hongyuan¹, JIN Ai⁵]]> WANG Ling¹, JIN Liang², DAI Yan³, QI Xiaoru⁴, YU Hongyuan¹, JIN Ai⁵ 302true ［Abstract］　Objective　To construct and assess a Nomogram model for lung cancer patients with concurrent deep vein thrombosis(DVT). Methods　The clinical data of 330 cases of lung cancer who were admitted to the Second People′s Hospital of Hefei Affiliated to Bengbu Medical University from January 2022 to January 2023 were retrospectively analyzed, and the patients were divided into DVT group(33 cases) and non-DVT group(297 cases) according to the occurrence of DVT at admission. LASSO regression analysis and multivariate logistic regression analysis were used to screen the risk factors of the lung cancer patients with concurrent DVT to construct a Nomogram model, and the model was internally validated to assess its accuracy, consistency and clinical utility. Results　Compared with the non-DVT group, the DVT group was older and had a greater proportion of smokers and users of antiangiogenic drugs, and had higher levels of leukocytes, neutrophils, red blood cell distribution width(RDW), neutrophil-lymphocyte ratio(NLR), lactate dehydrogenase(LDH), brain natriuretic peptide(BNP), D-dimer, prothrombin time(PT), C-reactive protein(CRP) and neuron-specific enolase(NSE), and lower levels of hemoglobin and albumin, and the differences were statistically significant(P<0.05). With the occurrence of DVT as the dependent variable, five risk factors of lung cancer complicated with DVT, including hypertension, smoking history, antiangiogenic drugs, D-dimer and albumin were screened by using LASSO regression and multivariate logistic regression and a Nomogram model was constructed based on these screened risk factors. The results of receiver operating characteristic(ROC) curve analysis indicated that the model had good discriminative ability［AUC(95%CI)=0.880(0.811-0.950)］. The calibration curves showed that the predicted results of the model had a good fit with the actual observed values. The results of Hosmer-Lemeshow goodness-of-fit test(χ²=6.469, P=0.595) further proved that the model had good consistency. The results of decision curve analysis(DCA) showed that the model had a relatively large potential for clinical application when the threshold probability was in the range of 0.07-0.95. The results of clinical impact curve(CIC) analysis showed that within the range of threshold probability, the number of the patients who were predicted to have DVT was always greater than the number of the patients who actually had DVT, suggesting that the Nomogram model could effectively identify the high-risk patients with DVT. Conclusion　The Nomogram model of lung cancer complicated with DVT constructed in this study has good predictive ability and clinical utility, which can help clinicians better identify high-risk patients and take appropriate intervention measures in time to improve the patients′ prognosis.]]> 2024/9/29 0:00:00 1, TANG Ming¹, CHANG Xindong¹, HE Mingfei¹, LIU Ying², YIN Shiwu¹]]> FANG Qingqing¹, TANG Ming¹, CHANG Xindong¹, HE Mingfei¹, LIU Ying², YIN Shiwu¹ 301true ［Abstract］　Objective　To observe the therapeutic effect of surgical excision combined with limbal stem cell transplantation on treatment of pterygium. Methods　A total of 180 patients with pterygium who were admitted to Qidong Hospital of TCM from January 2020 to December 2022 were recruited and divided into the Ologen collagen matrix implantation group(receiving surgical excision combined with Ologen collagen matrix implantation treatment, 145 eyes, 90 cases) and the limbal stem cell transplantation group(receiving surgical excision combined with limbal stem cell transplantation treatment, 147 eyes, 90 cases) by using random number table method. The postoperative refractive status, tear film function, comfort level, wound healing time, clinical efficacy and recurrence were compared between the two groups. Results　After surgery, the uncorrected visual acuity(UCVA) and tear break-up time(BUT) showed an increasing trend in both groups, while corneal astigmatism(CA) showed a decreasing trend, and the differences in the changing amplitudes between the two groups were statistically significant(P<0.05). At 6 months after surgery, the UCVA and BUT of the Ologen collagen matrix implantation group were lower than those of the limbal stem cell transplantation group, and the CA of the Ologen collagen matrix implantation group was higher than that of the limbal stem cell transplantation group, and the differences were statistically significant between the two groups(P<0.05). At 6 months after surgery, the comfort scores of the limbal stem cell transplantation group were significantly better than those of the Ologen collagen matrix implantation group in four dimensions, including photophobic tears, foreign body sensation, eye pain and hyperemia of the bulbar conjunctiva(P<0.05). The wound healing time of the limbal stem cell transplantation group was significantly shorter than that of the Ologen collagen matrix implantation group［(2.85±1.06)d vs (3.04±0.31)d; t=2.073, P=0.039］. The clinical efficacy rate of the limbal stem cell transplantation group was significantly higher than that of the Ologen collagen matrix implantation group(98.64% vs 93.10%; χ²=5.678, P=0.017). The recurrence rate at 6 months after surgery in the limbal stem cell transplantation group was lower than that in the Ologen collagen matrix implantation group(0.00% vs 4.14%; χ²=4.325, P=0.038). Conclusion　Surgical excision combined with limbal stem cell transplantation is superior to surgical excision combined with Ologen collagen matrix implantation in treatment of pterygium, and surgical excision combined with limbal stem cell transplantation is worth recommending.]]> 2024/9/29 0:00:00 WU Qin, YANG Chunlin, LU Yaoyao, CAO Donglei 300true ［Abstract］　Objective　To explore the effect of warming acupuncture combined with intrauterine perfusion of platelet-rich plasma(PRP) on the outcome of freeze-thaw embryo transfer in patients with thin endometrium of kidney deficiency and blood stasis type. Methods　One hundred and fifty-four patients with thin endometrium of kidney deficiency and blood stasis type who underwent in vitro fertilization and embryo transfer(IVF-ET) in the Reproductive Medicine Center of Qinzhou Maternal and Child Health Hospital from January 2020 to December 2022 were recruited. According to the patients′ treatment intentions, they were divided into group A(27 cases, receiving hormone replacement cycle), group B(43 cases, receiving hormone replacement cycle+warming acupuncture treatment), group C(48 cases, receiving hormone replacement cycle+intrauterine perfusion of PRP treatment) and group D(36 cases, receiving hormone replacement cycle+warming acupuncture+intrauterine perfusion of PRP treatment). On the third day after administering progesterone to transform the endometrium, 1 to 2 high-quality cleavage stage embryos were transplanted. The endometrial thickness on the day when progesterone was administered to transform the endometrium, clinical pregnancy rate, biochemical pregnancy rate and early abortion rate were compared among the four groups. Results　After treatment, the endometrial thickness in group B, group C and group D on the day when progesterone was administered to transform the endometrium increased compared with those in the previous cycle, and the differences were statistically significant(P<0.05), and the endometrial thickness in group B, group C and group D on the day when progesterone was administered to transform the endometrium was significantly greater than that in group A(P<0.05). However, there was no statistically significant difference among group B, group C and group D(P>0.05). There was no statistically significant difference in the number of transplanted embryos among the four groups. There were statistically significant differences in the clinical pregnancy rate and biochemical pregnancy rate among the four groups(P<0.05), and the clinical pregnancy rate and biochemical pregnancy rate in group D were the highest, which were 44.44% and 61.11%, respectively. There was no significant difference in the early abortion rate among the four groups(P>0.05). Conclusion　Warming acupuncture combined with intrauterine perfusion of PRP helps to improve the endometrial thickness and the outcome of freeze-thaw embryo transfer in patients with thin endometrium of kidney deficiency and blood stasis type.]]> 2024/9/29 12:29:45 LIN Chunlian, LIU Yanmei, ZHANG Zhao, WU Hongbo 299true ［Abstract］　Objective　To analyse the antigen detection results of 7 respiratory viruses［including respiratory syncytial virus(RSV), human adenovirus(HAdV), influenza virus-A(IFV-A), influenza virus-B(IFV-B), human parainfluenza virus-1(HPIV-1), human parainfluenza virus-2(HPIV-2) and human parainfluenza virus-3(HPIV-3)］ in pediatric patients with acute respiratory tract infection(ARTI) in Zhongshan area of Guangdong Province from 2016 to 2022. Methods　The clinical data of 60 265 pediatric patients with ARTI who were admitted to Boai Hospital of Zhongshan from January 2016 to December 2022 were retrospectively collected. Among the patients, 49 116 cases(81.50%, aged 29 days to 14 years) underwent pathogen examination and were included in this analysis. Nasopharyngeal swabs were used to collect the specimens on the day of admission or the next day. The antigens of RSV, HAdV, IFV-A, IFV-B, HPIV-1, HPIV-2 and HPIV-3 were detected by using direct immunofluorescence method. Results　Of the 49 116 pediatric patients with ARTI, 11 210 cases(22.82%) tested positive for at least 1 viral antigen. A total of 11 462 cases tested positive for viral antigens, and the detectable rates in descending order were 13.84% in RSV, 3.14% in IFV-A, 2.45% in HAdV, 1.76% in HPIV-3, 1.15% in IFV-B, 0.68% in HPIV-1 and 0.32% in HPIV-2. The detectable rate of RSV was the highest in 2020(19.15%) and the lowest in 2022(7.74%). The detectable rate of IFV-A in 2021 was 0.00%, and in the other observed years, the detectable rate of IFV-A fluctuated between 2.32% and 4.33%. The detectable rate of HAdV was the highest in 2019(4.43%) and the lowest in 2021(0.39%). The detectable rates of IFV-B, HPIV-1, HPIV-2 and HPIV-3 were low in each observed year. There were significant differences in the detectable rates of 7 viruses in different seasons(P<0.05). RSV infection were more common in summer and winter. HAdV infection was more common in summer. IFV-A infection occurred more frequently in winter and less frequently in autumn. The detectable rates of RSV and HPIV-3 were the highest in the age group of 29 days-<1 year, which were 22.32% and 2.90%, respectively. The detectable rates of IFV-A and IFV-B were the highest in the age group of 6-14 years, which were 6.30% and 3.17%, respectively. The detectable rate of HAdV was the highest in the age group of 3 years-<6 years(4.30%), and the detectable rate of HPIV-1 was the highest in the age group of 1 year-<3 years(0.85%). There were no significant differences in the detectable rates of HPIV-2 among different age groups(P>0.05). The detectable rate of RSV in males was higher than that in females, and the difference was statistically significant(P< 0.05). There were no statistically significant differences in the detectable rates of the other 6 viruses between different genders in the pediatric patients(P>0.05). RSV was mainly detectable in the pediatric patients with acute asthmatic bronchitis(28.17%). The detectable rates of IFV-A, HAdV and IFV-B were the highest in the pediatric patients with acute upper respiratory tract infection, and the detectable rates of HAdV, IFV-A and IFV-B were 3.66%, 4.91% and 1.70%, respectively. However, the detectable rate of HPIV-3 in the pediatric patients with acute upper respiratory tract infection was lower(1.20%). Conclusion　RSV, IFV-A and HAdV are the main pathogens for the pediatric patients with ARTI in Zhongshan area of Guangdong Province. Infants and males are the high-incidence groups of respiratory virus infection.]]> 2024/9/29 12:29:45 1,2,WANG Guilan^1,3, HUANG Juan³, WANG Bingjie³, LIU Xiangteng³]]> HUANG Ren^1,2,WANG Guilan^1,3, HUANG Juan³, WANG Bingjie³, LIU Xiangteng³ 298true ［Abstract］　Objective　To analyze the correlation of serum soluble CD73(sCD73) and S100 calcium-binding proteins A8/A9(S100A8/A9) levels with clinicopathological features and therapeutic prognosis of programmed death receptor-1(PD-1)/programmed death-ligand 1(PD-L1) inhibitors in patients with advanced endometrial cancer. Methods　A total of 142 patients with advanced endometrial cancer who were treated with PD-1/PD-L1 inhibitors in the First Hospital of Qiqihar from March 2020 to February 2021 were recruited. A total of 5 mL of fasting elbow venous blood was collected from the patients in the morning after admission and their blood serum were collected, and the patients′ serum levels of sCD73 and S100A8/A9 were detected by using enzyme-linked immunosorbent assay(ELISA). During the follow-up period, 58 patients died(death group) and 84 patients survived(survival group). The efficacy of serum sCD73 and S100A8/A9 levels in predicting the patients′ survival outcomes were evaluated by using receiver operating characteristic(ROC) curve. According to the optimal cut-off values of the ROC curve analysis, the patients were divided into sCD73 high expression group, sCD73 low expression group, S100A8/A9 high expression group and S100A8/A9 low expression group. The factors affecting the survival prognosis of the advanced endometrial cancer patients treated with PD-1/PD-L1 inhibitors were analyzed by using Cox regression. The correlation of serum sCD73 and S100A8/A9 expression levels with the patients′ clinicopathological features were analyzed. Results　The serum levels of sCD73 and S100A8/A9 in the survival group were lower than those in the death group, and the differences were statistically significant(P<0.05). The results of ROC curve analysis showed that serum sCD73 and S100A8/A9 could effectively predict the survival outcomes of the advanced endometrial cancer patients treated with PD-1/PD-L1 inhibitors(P<0.05), and the optimal cut-off values were 5.30 μg/L and 3.06 μg/mL, respectively. The survival prognosis in the sCD73 low expression group and the S100A8/A9 low expression group was better than that in the sCD73 high expression group and the S100A8/A9 high expression group, respectively(log-rank test: χ²=91.367, P<0.001; χ²=69.852, P<0.001). The results of multivariate Cox regression analysis showed that higher serum levels of sCD73［HR(95%CI)=2.511(1.497-4.214), P<0.001］ and S100A8/A9［HR(95%CI)=1.806(1.106-2.948), P=0.018］ were independent risk factors for poor survival prognosis in the patients. The proportions of poor differentiation in the sCD73 high expression group and the S100A8/A9 high expression group were significantly greater than those in the sCD73 low expression group and the S100A8/A9 low expression group, respectively(P<0.05). Conclusion　High levels of serum sCD73 and S100A8/A9 in the patients with advanced endometrial cancer may indicate poor tumor differentiation and are risk factors for poor survival prognosis in the patients treated with PD-1/PD-L1 inhibitors.]]> 2024/9/29 0:00:00 1, SHAO Kai², YANG Fan³, CAI Yujie¹, YANG Liyuan¹]]> JIA Tianhan¹, SHAO Kai², YANG Fan³, CAI Yujie¹, YANG Liyuan¹ 297true ［Abstract］　Objective　To investigate and analyze FZD4 gene mutations in paediatric patients with familial exudative vitreoretinopathy(FEVR) in Chongqing area, and to analyze the relationship between genotype and phenotype. Methods　A total of 90 pedigrees(300 cases) of the paediatric patients who were diagnosed with FEVR after neonatal eye disease screening in Chongqing Health Center for Women and Children from January 2019 to December 2022 were recruited. All the members of the 90 pedigrees came from Chongqing area. All the paediatric patients underwent fundus examination by using wide-field fundus imaging system of RetCamIII, and 10 pathogenic genes of FEVR(including FZD4, NDP, LRP5, TSPAN12, ZNF408, CFTR, ADGRG2, SLC9A3, KIF11 and CAPN5) were detected in the genomic DNA of leukocytes by high-throughput RNA sequencing(RNA-Seq). The relationship between genotype and phenotype in the patients was analyzed. Results　Among the 90 probands, 4 cases(4.44%) were found to have FZD4 gene mutations, including 2 cases of spontaneous mutations and 2 cases of paternal source mutations, and these types of mutations were non-synonymous mutations. All the 4 paediatric patients had binocular diseases. According to FEVR staging, 3 eyes were in stage 1, 3 eyes in stage 2A and 2 eyes in stage 2B. According to FEVR zoning, 2 eyes were located in zone 2 and 6 eyes in zone 3. There was 1 case in combination with CAPN5 gene mutation. The chromosomal mutation sites discovered in this study included chr11-86662209, chr11-86663485, chr11-86665923 and chr11-86662893. Conclusion　This study expands the mutation spectrum of FZD4 gene and confirms that there may be inconsistency between genotype and phenotype in patients with FEVR.]]> 2024/8/30 0:00:00 ZHOU Wenke, HE Liying, TAO Xueying, CEN Chao, GONG Liyan, LI Qi, LI Chunmei, LIU Ya 296true ［Abstract］　Objective　To explore the effect of cancer-testis antigen OY-TES-1 expression on the polarization of M2 macrophages in glioblastoma(GBM). Methods　Human GBM cell U251 and human peripheral blood mononuclear cell THP-1 were cultured in vitro to construct a stable transfection cell line U251 that stably down-regulated OY-TES-1(U251-SH-OY-TES-1 group), and U251 cells transfected with unrelated sequences were used as the controls(U251-SH-NC group). Forty-eight hours later, the supernatant of the two groups was co-cultured with THP-1 cells. THP-1 cells to which the supernatant of the U251-SH-NC group was added were taken as the SH-NC group, and THP-1 cells to which the supernatant of the U251-SH-OY-TES-1 group was added were taken as the SH-OY-TES-1 group. The relative expression levels of OY-TES-1 mRNA and protein in the two groups were detected by using real-time fluorescence quantitative polymerase chain reaction(RT-qPCR) and Western blotting, respectively, and the transfection efficiency was determined. The expression levels of human interleukin-10(IL-10) and human transforming growth factor-β(TGF-β) in the culture solution were detected by enzyme-linked immunosorbent assay(ELISA). The expression level of CD206 was detected by flow cytometry. Results　The detection results of RT-qPCR and Western blotting method showed that the relative expressions of OY-TES-1 mRNA and protein in the U251-SH-OY-TES-1 group were lower than those in the U251-SH-NC group, and the differences were statistically significant(P<0.05). The results of ELISA showed that the levels of TGF-β and IL-10 in the culture solution of the two groups increased with the increase of culture time. On day 0 and day 2, the levels of TGF-β in the culture solution of the SH-OY-TES-1 group were significantly lower than those of the SH-NC group(P<0.05), and there were no significant differences in the levels of IL-10 on day 0 and day 2 between the two groups(P>0.05). On day 4, the levels of TGF-β and IL-10 in the culture solution of the SH-OY-TES-1 group were significantly lower than those of the SH-NC group(P<0.05). The results of flow cytometry showed that compared with that in the SH-NC group, the level of CD206 in the SH-OY-TES-1 group decreased, and the difference was statistically significant(P<0.05). Conclusion　The high expression of OY-TES-1 in GBM can promote the polarization of M2 macrophages.]]> 2024/8/30 0:00:00 1, LIANG Guo¹, LI Feng¹, NONG Weixia^1,2, ZHANG Qingmei^1,2, LUO Bin¹, XIE Xiaoxun^1,2]]> ZHAO Zhenkai¹, LIANG Guo¹, LI Feng¹, NONG Weixia^1,2, ZHANG Qingmei^1,2, LUO Bin¹, XIE Xiaoxun^1,2 295true ［Abstract］　Objective　To explore the application value of a novel indicator of HROX index in early diagnosis and treatment of severe pneumonia in children. Methods　The clinical data of 198 pediatric patients with pneumonia who were admitted to Suzhou Wujiang District Children′s Hospital from February 2020 to December 2022 were retrospectively collected, including 98 cases of severe pneumonia(severe pneumonia group) and 100 cases of common pneumonia(common pneumonia group). The general clinical data, laboratory indicators, imaging indicators and HROX index［HROX index=heart rate×respiratory rate/oxygen saturation(SpO₂)］ were compared between the two groups. The efficacy of HROX index in early diagnosis of severe pneumonia in children and in predicting the need for oxygen therapy in pediatric patients during hospitalization was analyzed. Results　Compared with the common pneumonia group, the severe pneumonia group had higher incidence rates of shortness of breath, wheezing, inspiratory three-concave sign and pleural effusion, and longer hospital stay, higher levels of C-reactive protein(CRP), procalcitonin(PCT), interleukin-6(IL-6), lactate dehydrogenase(LDH), D-dimer and HROX index, and lower levels of neutrophil percentage(NEU) and hemoglobin(Hb), and the differences were statistically significant(P<0.05). The results of multivariate logistic regression analysis showed that higher HROX index［OR(95%CI)=1.211(1.038-1.414)］ and PCT［OR(95%CI)=1.570(1.142-2.157)］, IL-6［OR(95%CI)=1.028(1.001-1.057)］ and D-dimer［OR(95%CI)=18.697(1.091-320.405)］ levels were independent risk factors for promoting the occurrence of severe pneumonia(P<0.05). The results of receiver operating characteristic(ROC) curve analysis showed that HROX index was helpful for early diagnosis of severe pneumonia［AUC(95%CI)=0.907(0.863-0.951), P<0.001］, and could predict the need for oxygen therapy during hospitalization in the pediatric patients［AUC(95%CI)=0.902(0.857-0.947), P=0.003］. Conclusion　HROX index helps clinicians diagnose severe pneumonia early and predicts the need for oxygen therapy during hospitalization in pediatric patients.]]> 2024/8/30 0:00:00 YAN Qiuli, ZHANG Fangyuan, CHEN Yingqing, SHI Jinjin, NIU Wensi 294true ［Abstract］　Objective　To observe the clinical effectiveness of open preperitoneal repair in treatment for adults with small umbilical hernia. Methods　The clinical data of 88 patients with small umbilical hernia who were admitted to Jin Xiang People′s Hospital Affiliated to Jining Medical University from January 2018 to October 2022 were retrospectively analyzed, and the patients were divided into observation group(receiving open preperitoneal repair, n=42) and control group(receiving traditional suture repair, n=46) according to different surgical methods. The duration of surgery, amount of intraoperative blood loss, postoperative Visual Analogue Scale(VAS) score, incision healing grade, length of hospital stay, as well as the postoperative seroma, localized paresthesia, chronic pain and recurrence were compared between the two groups. Results　The operations of the 88 patients were successfully completed. The duration of surgery in the observation group was significantly longer than that in the control group［(51.24±12.64)min vs (42.04±11.88)min; t=3.419, P=0.001］. The postoperative VAS scores showed a downward trend in both groups(P<0.05). The VAS scores of the observation group were significantly lower than those of the control group on the 1st and the 2nd day after surgery(P<0.05), and there were no significant differences in the VAS scores between the two groups on the 3rd day after surgery(P>0.05). There were no significant differences in the amount of intraoperative blood loss, incision healing grade, as well as the incidence rates of postoperative seroma, chronic pain and localized paresthesia between the two groups(P>0.05). The median follow-up time was 19 months in the observation group and 17 months in the control group. During the follow-up period, there were no cases of recurrence in the observation group and 5 cases relapsed in the control group, and the difference was statistically significant between the two groups(log-rank test: χ²=4.833, P=0.027). Conclusion　Open preperitoneal repair is safe and effective in treatment for adults with small umbilical hernia, and is worthy of clinical recommendation.]]> 2024/8/30 10:29:46 YANG Yingxin, SHAN Changling, ZHANG Lei, WANG Zhiguo, WANG Songsong, ZHANG Hua 293true ［Abstract］　Objective　To observe the clinical efficacy of magnetic resonance imaging(MRI) localization of seminal vesicle combined with F4.8 visual puncture super-mini nephroscope in treating seminal vesicle diseases. Methods　The clinical data of 17 patients with hematospermia who were admitted to Department of Urology, the Seventh Affiliated Hospital of Southern Medical University from January 2021 to March 2023 were retrospectively analyzed. MRI was performed on the seminal vesicles of all the patients before operation, and the seminal vesicle lumen was examined and surgically treated with F4.8 visual puncture super-mini nephroscope. The changes in semen-related indicators before and after surgery, and the sympton improvement and the occurrence of complications after surgery were recorded. Results　The operations of all the 17 patients were successfully completed, and the duration of surgery was 43-57(48.65±4.94)minutes. The intraoperative findings indicated that there were 3 cases of prostatic utricle calculi, 8 cases of seminal vesicle calculi, 1 case of seminal vesicle cysts, 2 cases of ejaculatory duct cysts, 1 case of seminal vesicle polyps and 2 cases of seminal vesiculitis. There were no complications such as rectal injury, external urethral sphincter injury and peripheral organ injury during the operation. The patients were reexamined 3 months after surgery, and all the patients had no gross hematospermia, and the improvement rate of the symptoms such as lower abdominal distending pain and ejaculation pain was 94.12%(16/17). After reexamination, the postoperative semen-related indicators(semen volume, semen pH value, sperm concentration, total sperm motility, seminal plasma fructose, red blood cells and white blood cells) were significantly improved compared with the preoperative semen-related indicators. After surgery, there were no complications such as retrograde ejaculation, urinary incontinence, urinary stammering, decrements in sexual function and epididymitis. Conclusion　The treatment of seminal vesicle diseases with MRI localization of seminal vesicle combined with F4.8 visual puncture super-mini nephroscope has the advantages of less trauma, fewer complications and good clinical effect, and is worthy of clinical promotion and application.]]> 2024/8/30 10:29:46 ZOU Feng, ZHANG Guofei, ZHANG Wansong, QIU Chunming, SUN Canbiao, YANG Yong, ZHAN Shiyue 292true ［Abstract］　Objective　To observe the application effect of group social interaction intervention combined with precise family intervention in paediatric patients with autism spectrum disorder(ASD). Methods　A total of 52 paediatric patients with ASD who were admitted to the Department of Rehabilitation of Xuzhou Children′s Hospital Affiliated to Xuzhou Medical University from May 2022 to December 2022 were recruited and divided into control group and observation group by random number table method, with 26 cases in each group. The control group received routine group social interaction intervention training, and the observation group received routine group social interaction intervention combined with precise family intervention. The intervention time was 6 months in both groups. The scores of Childhood Autism Rating Scale(CARS), Autism Treatment Evaluation Checklist(ATEC), and Neuropsychological Development Examination Table for Children and Psychoeducational Profile-Third Edition(PEP-3) were compared between the two groups before and after intervention. Results　After intervention, ATEC and CARS scores in both groups were significantly lower than those before intervention(P<0.05), and the ATEC and CARS scores in the observation group were lower than those in the control group, and the differences were statistically significant(P<0.05). The scores of adaptive ability, language and individual-social interaction behavior of the Neuropsychological Development Examination Table for Children, and the scores of social interaction ability, self-care ability of daily living and cognitive ability of PEP-3 in both groups were significantly higher compared with those before intervention(P<0.05), and the scores in the observation group were higher than those in the control group, and the differences were statistically significant(P<0.05). After intervention, the gross motor and fine motor scores of the Neuropsychological Development Examination Table for Children in both group were significantly higher compared with those before intervention(P<0.05), but there were no statistically significant differences between the two groups(P>0.05). Conclusion　Group social interaction intervention combined with precise family intervention can effectively improve the social interaction ability, cognitive ability and self-care ability of daily living in paediatric patients with ASD.]]> 2024/8/30 10:29:46 1, GONG Zunke², BAO Kexiu¹, WU Ye¹, WANG Min¹]]> GONG Tiantian¹, GONG Zunke², BAO Kexiu¹, WU Ye¹, WANG Min¹ 291true ［Abstract］　Objective　To explore the correlation of long noncoding RNA(lncRNA) X-inactive specific transcript(XIST)(lncRNA-XIST) and lncRNA-MALAT1 with insulin resistance and pregnancy outcome after ovulation induction therapy in infertile patients with polycystic ovary syndrome(PCOS). Methods　A total of 125 infertile patients with PCOS who underwent ovulation induction therapy in Tangshan Maternal and Child Health Hospital from February 2020 to January 2022 were recruited as PCOS infertility group, and other 125 healthy women who underwent physical examination during the same period were selected as control group. The serum levels of lncRNA-XIST and lncRNA-MALAT1, and homeostasis model assessment-insulin resistance(HOMA-IR) index were compared between the two groups. Pearson correlation analysis was used to explore the correlation of serum levels of lncRNA-XIST and lncRNA-MALAT1 with HOMA-IR. Multivariate logistic regression analysis was used to explore the factors affecting the pregnancy outcome after ovulation induction therapy in infertile patients with PCOS. Results　The serum levels of lncRNA-XIST and HOMA-IR in the PCOS infertility group were higher than those in the the control group, and the lncRNA-MALAT1 level in the PCOS infertility group was lower, and the differences were statistically significant(P<0.05). HOMA-IR was positively correlated with serum lncRNA-XIST level(r=0.689, P<0.001), and negatively correlated with serum lncRNA-MALAT1 level(r=-0.771, P<0.001). The cumulative clinical pregnancy rate of the PCOS infertility patients after ovulation induction therapy was 43.20%(54/125). The results of multivariate logistic regression analysis showed that higher luteinizing hormone(LH)/follicle stimulating hormonec(FSH) ratio［OR(95%CI)=1.121(1.005-1.278)］ and serum level of lncRNA-XIST［OR(95%CI)=1.252(1.014-1.449)］ were independent risk factors for promoting non-pregnancy in the PCOS infertility patients after treatment(P<0.05), and higher serum levels of anti-Müllerian hormone(AMH)［OR(95%CI)=0.518(0.348-0.771)］ and lncRNA-MALAT1［OR(95%CI)=0.394(0.238-0.652)］ were protective factors for promoting pregnancy in the PCOS infertility patients after treatment(P<0.05). Conclusion　Serum lncRNA-XIST expression is high and lncRNA-MALAT1 expression is low in PCOS infertility patients, and the levels of the two indicators are closely related to insulin resistance and pregnancy outcome after ovulation induction therapy.]]> 2024/8/30 10:29:46 LEI Jinmei, FENG Yuhuan, ZHANG Ou, GAO Shang, CHEN Wei 290true ［Abstract］　Objective　To analyze the clinical characteristics and gene mutation characteristics of proline-rich transmembrane protein 2(PRRT2) gene mutation-related epilepsy in children. Methods　The clinical data of 17 pediatric patients with PRRT2 gene mutation-related epilepsy who were admitted to Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region from January 2015 to August 2020 were retrospectively analyzed, and their clinical characteristics and gene mutation characteristics were summarized and analyzed. Results　Among the 17 pediatric patients, 9 cases were male and 8 cases were female, and the median onset age of the patients was 5 months. There were various types of epileptic seizures, and 10 cases had cluster seizures at the beginning of the disease. The patients were diagnosed with benign infantile epilepsy(BIE) in 8 cases, benign familial infantile epilepsy(BFIE) in 4 cases, infantile spasms(IS) in 1 case, and BFIE+paroxysmal kinesigenit dyskinesia(PKD) in 1 case. After monotherapy with anti-seizure medications, the condition of the most pediatric patients was well controlled. Up to the last follow-up, 16 patients′ seizures were relieved, and 1 patient still had seizures. Cognitive development was delayed in 4 patients. The results of genetic testing showed that 8 cases of the 17 cases had global deletion of PRRT2 gene, and 9 cases had point mutation of PRRT2 gene, all of which were frameshift mutations, and the mutation site of 8 cases was c.640_641insC. There were 7 cases of familial inherited mutation and 2 cases of de novo mutation. Conclusion　The majority of PRRT2 gene mutation-related epilepsy are in onset within 6 months after birth in pediatric patients, and cluster seizures are the prominent features at the beginning of the disease. There are various types of epileptic seizures with varying degrees of severity. Monotherapy with anti-seizure medications can effectively control epileptic seizures. A small number of the pediatric patients have different degrees of cognitive retardation. The site of c.640_641insC may be the hot spot mutation site of PRRT2 gene.]]> 2024/8/30 10:29:46 RUAN Yiyan, CHEN Yuyi, WANG Jinqiu, CHEN Yin, FENG Juntan, WEI Fengping, SONG Lingli, LIANG Lusi 289true ［Abstract］　Objective　To systematically evaluate the value of tumor mutation burden(TMB) predicting the efficacy of immune checkpoint inhibitors(ICIs) in patients with gastrointestinal malignancies. Methods　A total of 963 papers of the relevant literature in PubMed, Web of Science and Cochrane Library databases from the time of establishment to December 1 2021 were searched, and 12 papers of the relevant literature(including 1 126 cases of gastrointestinal malignancies treated with ICIs) were finally included for Meta-analysis. The main evaluation indicators included objective response rate(ORR), progression-free survival(PFS), and overall survival(OS). Results　In the patients treated with ICIs, the patients with high TMB had longer OS［HR(95%CI)=0.71(0.51-0.97), P=0.031］, and higher ORR［OR(95%CI)=4.22(2.58-6.90), P<0.001］ compared with those with low TMB. However, there was no significant difference in PFS between the two group of the patients［HR(95%CI)=0.77(0.48-1.23), P=0.276］. Conclusion　The gastrointestinal malignancy patients with high TMB are more likely to benefit from the treatment of ICIs.]]> 2024/8/30 10:29:46 1, CHEN Zhixin², SHEN Yongqi¹, QIN Junyun¹, LIN Deshuai¹, LIU Jin′e¹, MO Que¹, HAN Chaowen¹]]> LAI Xiangli¹, CHEN Zhixin², SHEN Yongqi¹, QIN Junyun¹, LIN Deshuai¹, LIU Jin′e¹, MO Que¹, HAN Chaowen¹ 288true ［Abstract］　Objective　To observe the therapeutic effect of AcoStream Thrombectomy System combined with catheter directed thrombolysis(CDT) in treatment of lower extremity deep venous thrombosis(LDVT) with iliac vein compression syndrome(IVCS). Methods　The clinical data of 71 patients having LDVT with IVCS who were admitted to First People′s Hospital of Shangqiu from June 2020 to December 2022 were retrospectively analyzed. The patients were divided into observation group(receiving treatment of AcoStream Thrombectomy System combined with CDT, 29 cases) and control group(receiving treatment of CDT, 42 cases) according to different treatment regimens. The stent implantations, changes in hemoglobin(Hb) values before and after surgery, dosage of urokinase, length of hospital stay, peak time of D-dimer, clearance rate of thrombus, difference in thigh circumference, difference in calf circumference and Villalta scores were compared between the two groups. Results　The dosage of urokinase in the observation group was lower than that in the control group, and the changes in Hb values before and after surgery in the observation group were larger than those in the control group, and the length of hospital stay and the peak time of D-dimer in the observation group were shorter than those in the control group, and the differences were statistically significant(P<0.05). The thrombolysis grade in the observation group was better than that in the control group, and the clearance rate of thrombus in the observation group was higher than that in the control group, and the Villalta scores in the observation group were lower than those in the control group, and the differences were statistically significant(P<0.05). The difference in thigh circumference and the difference in calf circumference 6 months after operation in both groups were significantly decreased compared with those before operation in both groups(P<0.05), but the differences were not statistically significant between the two groups(P>0.05). Conclusion　AcoStream Thrombectomy System combined with CDT is definitely effective in treating LDVT with IVCS, with lower risk of hemorrhage and significant symptom relief for the patients.]]> 2024/8/30 0:00:00 SUO Feifei, LI Jingjing, ZHAI Gang, PANG Hongyong, TIAN Ke 287true ［Abstract］　Objective　To survey the prevalence rate of nosocomial infection in a specialized hospital for geriatric diseases in Guangxi from 2013 to 2022, and analyze the results. Methods　An annual survey on nosocomial infection was conducted in Jiangbin Hospital of Guangxi Zhuang Autonomous Region every year from 2013 to 2022. All the clinically hospitalized patients were selected as the survey subjects from 0:00 to 24:00 on a certain day in July. The survey subjects included the patients who were discharged from the hospital or transferred to other hospitals on the survey day, and those who were admitted to the hospital were excluded from the survey on the same day. The data of nosocomial infection monitoring and management in each year were retrospectively analyzed and summarized. Results　From 2013 to 2022, among the 11 078 patients receiving the survey, 223 cases of nosocomial infection occurred, totaling 234 times in 223 cases. The overall prevalence rate of the nosocomial infection was 2.01%(223/11 078), and the incidence rate of 234 times in 223 cases was 2.11%(234/11 078). The rate of infection times in infected cases was highest in 2014(3.72%), and lowest in 2021(1.14%), showing an overall downward trend. The clinical department with the highest prevalence rate of nosocomial infection was the Department of Respiratory Medicine(13.44%), and the infection site was mainly in the lower respiratory tract, followed by the urinary tract, upper respiratory tract and gastrointestinal tract. A total of 190 strains of pathogenic bacteria were in the infected patients detected in this survey, including 135 strains of Gram-negative bacteria(71.05%), 44 strains of Gram-positive bacteria(23.16%) and 11 strains of fungi(5.79%). The results of multivariate logistic regression analysis showed that age ≥ 65 years［OR(95%CI)=1.790(1.345-2.382), P<0.001］ and use of antibiotics［OR(95%CI)=1.912(1.445-2.530), P<0.001］ were independent risk factors for the nosocomial infection(P<0.05). Conclusion　The prevalence rate of nosocomial infection in the hospital shows a downward trend, and the prevention and control work of nosocomial infection in the hospital has achieved certain results. However, it is still necessary to strengthen managements for key departments, susceptible infection sites and high-risk populations.]]> 2024/8/30 10:29:46 WEI Jinhua, WU Juan, ZHENG Dan 286true ［Abstract］　Objective　To explore the effect of preoperative neutrophil to prealbumin ratio index(NPRI) level on the occurrence of short-term complications in patients undergoing laparoscopic radical surgery for colorectal cancer. Methods　The clinical data of 302 patients who were admitted to Jiangsu Taizhou People′s Hospital Affiliated to Nanjing Medical University from June 2015 to June 2017 and underwent laparoscopic radical surgery for colorectal cancer were retrospectively analyzed. The patients were divided into complication group(n=49) and non-complication group(n=253) according to the occurrence of short-term complications after surgery. Multivariate logistic regression was used to analyze the factors affecting the occurrence of short-term complications after surgery in the patients. The receiver operating characteristic(ROC) curve was used to analyze the efficacy of preoperative NPRI level in predicting the occurrence of short-term complications after surgery. The relationship between preoperative NPRI level and clinical features, as well as the severity of postoperative complications in the patients was analyzed. Results　Of the 302 patients, 49 patients developed one complication or more complications for a total of 59 complications. According to the Clavien-Dindo grading system, among the 59 complications, 27 complications were grade Ⅰ, and 11 complications were grade Ⅱ, and 6 complications were grade Ⅲa, and 9 complications were grade Ⅲb, and 6 complications were grade Ⅳ. The results of multivariate logistic regression analysis showed that a history of abdominal surgery［OR(95%CI)=2.708(1.077-6.810)］, long operation time［OR(95%CI)=1.060(1.037-1.084)］, CEA≥ 5 ng/mL［OR(95%CI)=3.245(1.395-7.549)］ and higher preoperative NPRI level［OR(95%CI)=1.530(1.230-1.904)］ were independent risk factors for the occurrence of short-term complications in the patients after receiving laparoscopic radical surgery for colorectal cancer. The results of ROC curve analysis showed that preoperative NPRI level could effectively predict the occurrence of short-term complications in the patients after receiving laparoscopic radical surgery for colorectal cancer［AUC(95%CI)=0.781(0.700-0.848), P<0.001］, and the optimal cut-off value was 3.94, with the corresponding sensitivity and specificity being 59.20% and 85.40%, respectively. Compared with the low NPRI group(<3.94), the high NPRI group(≥3.94) had older age, lower preoperative hemoglobin level, longer operation time, and a greater proportion of patients with tumor located in the colon, tumor maximum diameter ≥5 cm and tumor pathological stage Ⅲ, and the differences were statistically significant(P<0.05). Compared with the low NPRI group, the high NPRI group had a higher incidence of postoperative complications(χ²=47.725, P<0.05) and a higher risk of serious complications(χ²=4.639, P=0.031). Conclusion　Higher preoperative NPRI level is a risk factor for short-term complications in patients undergoing laparoscopic radical surgery for colorectal cancer and is associated with the severity of complications.]]> 2024/8/3 0:00:00 1, HU Haoran², ZHOU Yaxing³, YUAN Yin³, WANG Honggang³]]> XIA Yong¹, HU Haoran², ZHOU Yaxing³, YUAN Yin³, WANG Honggang³ 285true ［Abstract］　Objective　To analyze the clinical significance of prenatal sequential screening for hereditary deafness gene carriers. Methods　A total of 9 391 pregnant women who underwent mutational site screening for hereditary deafness genes in Beijing Obstetrics and Gynecology Hospital, Capital Medical University from May 2022 to December 2022 were selected. The hereditary deafness genes were detected by using microfluidic chip method and the carrying rate of these hereditary deafness genes in the pregnant women was analyzed. The spouses of the pregnant women with GJB2 gene and SLC26A4 gene variants detected were screened for the same pathogenic genes by using targeted high-throughput sequencing. When both sides of the spouses were detected with the same pathogenic gene variants, prenatal diagnosis of the pathogenic gene was recommended for their fetus. For the pregnant women with GJB3 gene variant, audiological evaluation, genetic counseling and follow-up were recommended. For the pregnant women with mitochondrial 12S rRNA gene variant detected, genetic counseling and medication guidance were provided. Results　Among the 9 391 pregnant women, 1 002 cases were found to be carriers of hereditary deafness gene mutation, and the carrying rate was 10.67%. The carrying rates of GJB2, SLC26A4, GJB3 and mitochondrial 12S rRNA mutations were 7.93%, 1.99%, 0.28% and 0.15%, respectively. The mutations were verified by Sanger sequencing and the coincidence rate was 99.80%. The spouses of 293 carriers of GJB2 gene or SLC26A4 gene mutations were screened sequentially. Among these spouses, 4 couples were found to carry the disease-causing mutation site of the same gene. After sequencing of amniotic fluid cells, 1 case was found to carry GJB2 c.235 del C homozygous mutation, and 1 case was found to carry SLC26A4 c.919-2 A>G homozygous mutation and the other 2 cases were found to carry heterozygous mutations. Conclusion　Prenatal sequential screening of deafness gene carriers can not only screen the carriers of hereditary deafness gene mutations, but also identify the individuals who are sensitive to drug-induced deafness, thereby achieving early diagnosis, early intervention and timely warning of deafness in fetuses.]]> 2024/8/3 12:02:39 LI Shanshan, ZHANG Meng, CHEN Yujiao, YAN Yousheng, WANG Yipeng 284true ［Abstract］　Objective　To explore the application value of ¹⁸F-DOPA and ¹⁸F-FP-CIT contrast agents in diagnosing early Parkinson′s disease(PD) and assessing the severity of the disease in positron emission tomography/computed tomography(PET/CT). Methods　The clinical data of 119 PD patients(PD group) admitted to Department of Neurology, Sichuan Academy of Medical Sciences·Sichuan Provincial People′s Hospital from January 2022 to March 2023 were retrospectively analyzed. Other 28 individuals receiving healthy physical examination and matching the age and gender of the patients in the PD group during the same period were selected as the control group. Both the PD group and the control group received PET/CT examination. In the PD group, 50 cases received a contrast agent of ¹⁸F-DOPA(¹⁸F-DOPA-PD group) and 69 cases received a contrast agent of ¹⁸F-FP-CIT(¹⁸F-FP-CIT-PD group). In the control group, 14 cases received a contrast agent of ¹⁸F-DOPA(¹⁸F-DOPA- control group) and 14 cases received a contrast agent of ¹⁸F-FP-CIT(¹⁸F-FP-CIT- control group). The general clinical data, Visual Analogue Scale scores, and striatum caudate nucleus and putamen uptake indices were compared among all groups. The correlation of striatum caudate nucleus and putamen uptake indices with age, course of disease and Unified Parkinson′s Disease Rating Scale Part Ⅲ(UPDRS Ⅲ) scores in the PD patients was explored. Results　The striatum caudate nucleus and putamen uptake indices in the ¹⁸F-DOPA-PD group and the ¹⁸F-FP-CIT-PD group were significantly lower than those in the control group(P<0.05), and the decreases in the striatum caudate nucleus and putamen uptake indices on the contralateral side of the onset side were more significant than those on the onset side(P<0.05). Regardless of whether ¹⁸F-DOPA contrast agent or ¹⁸F-FP-CIT contrast agent was used, the striatum caudate nucleus and putamen uptake indices in the patients with mid- to late-stage PD were lower than those in the patients with early-stage PD, and the differences were statistically significant(P<0.05). In the ¹⁸F-DOPA-PD group, the uptake indices of the striatum caudate nucleus, anterior putamen, middle and posterior section of the putamen on the onset side and the contralateral side were negatively correlated with the patients′ age, course of disease and UPDRS Ⅲ scores(P<0.05). In the ¹⁸F-FP-CIT-PD group, the uptake indices of the striatum caudate nucleus, anterior putamen, middle and posterior section of the putamen on the onset sides and the contralateral sides of the patients were negatively correlated with the patients′ course of disease and UPDRS Ⅲ scores(P<0.05), but were not significantly correlated with the patients′ age(P>0.05). Conclusion　Both ¹⁸F-DOPA and ¹⁸F-FP-CIT contrast agents can reflect the degeneration of dopaminergic neurons in the brain of PD patients in PET/CT, which are helpful for early screening of PD patients and assessment of the disease progression.]]> 2024/8/3 0:00:00 1, DENG Yujiao², XIONG Chanyu¹, LUO Yongjie^1,3]]> TANG Yazhou¹, DENG Yujiao², XIONG Chanyu¹, LUO Yongjie^1,3 283true ［Abstract］　Objective　To analyze the effects of reproductive tract infections on the expressions of matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9(MMP-9) and the adverse maternal-infant outcomes in pregnant women with term premature rupture of membrane(tPROM) and in pregnant women with preterm premature rupture of membrane(pPROM). Methods　A total of 110 pregnant women with premature rupture of membrane(PROM) who saw a doctor in Taihe Hospital, Hubei University of Medicine from June 2021 to June 2023 were recruited as the study subjects. According to the situation of reproductive tract infections and gestational weeks, the patients were divided into pPROM infection group(34 cases), pPROM non-infection group(26 cases), tPROM infection group(34 cases) and tPROM non-infection group(16 cases). The general data, the levels of MMP-2 and MMP-9 expressions in cervical secretions, and adverse maternal-infant outcomes were compared among the four groups. Multivariate logistic regression was used to analyse the influencing factors of adverse maternal-infant outcomes in the PROM pregnant women. Results　There were no significant differences in age, gravidity, body mass index, smoking history and alcohol drinking history among the four groups(P>0.05). The levels of MMP-2 and MMP-9 expressions in cervical secretions were significantly different among the four groups(P<0.05), and the levels of MMP-2 and MMP-9 expressions in cervical secretions in the pPROM infection group were higher than those in the pPROM non-infection group and the tPROM non-infection group, and the levels of MMP-2 and MMP-9 expressions in cervical secretions in the tPROM infection group were higher than those in the tPROM non-infection group, and the differences were statistically significant(P<0.05). The overall incidence rates of chorioamnionitis and adverse maternal-infant outcomes were significantly different among the four groups(P<0.05), and the incidence rate of chorioamnionitis in the pPROM infection group was higher than that in the tPROM infection group and the tPROM non-infection group, and the overall incidence rate of adverse maternal-infant outcomes in the pPROM infection group was higher than that in the pPROM non-infection group and the tPROM non-infection group, and the differences were statistically significant(P<0.05). The results of multivariate logistic regression analysis showed that reproductive tract infections and higher levels of MMP-2 and MMP-9 expressions in cervical secretions were independent risk factors of adverse maternal-infant outcomes in the PROM pregnant women(P<0.05). Conclusion　Reproductive tract infections can increase the levels of MMP-2 and MMP-9 expressions in cervical secretions of pregnant women with tPROM and in cervical secretions of pregnant women with pPROM. Reproductive tract infections and higher levels of MMP-2 and MMP-9 expressions in cervical secretions are independent risk factors of adverse maternal-infant outcomes in PROM pregnant women.]]> 2024/8/3 12:02:39 1, CHENG Mumo², CHEN Lingling¹, XIE Zonglin¹, YAN Xinyu¹, ZHANG Zhijun¹]]> LI Jie¹, CHENG Mumo², CHEN Lingling¹, XIE Zonglin¹, YAN Xinyu¹, ZHANG Zhijun¹ 282true ［Abstract］　Objective　To observe the application effect of ultrasound-guided “bow tie sign” fascia iliaca block technique on analgesia after hip joint surgery. Methods　A total of 60 patients with hip fractures who underwent elective total hip replacement or artificial femoral head replacement in Tongling People′s Hospital from January 2020 to May 2023 were recruited and divided into control group and observation group by random number table method, with 30 cases in each group. The control group received postoperative intravenous analgesia, and the observation group underwent ultrasound-guided “bow tie sign” fascia iliaca block technique for analgesia based on the analgesia regimen of the control group. Postoperative opioid consumption, time of ambulation, length of hospital stay and Harris scores at discharge were compared between the two groups. The scores of passive Visual Analogue Scale(PVAS) and initiative Visual Analogue Scale(IVAS) were compared between the two groups at the time points of 2 hours(T₀), 6 hours(T₁), 12 hours(T₂), 24 hours(T₃) and 48 hours(T₄) after surgery. The sleep satisfaction, analgesia satisfaction and postoperative complication rate were compared between the two groups. Results　Compared with the control group, the observation group had less opioid dosage added within 48 hours after surgery, earlier time of ambulation, shorter length of hospital stay, and higher Harris scores at discharge, and the differences were statistically significant(P<0.05). The postoperative PVAS scores and IVAS scores showed a downward trend in both groups, and the magnitudes of decreases in the postoperative PVAS scores and IVAS scores were greater in the observation group(P<0.05). From the time point of T₁ to the time point of T₄, the PVAS scores and IVAS scores of the observation group were significantly lower than those of the control group(P<0.05). The sleep quality satisfaction rates 24 hours and 48 hours after surgery in the observation group were higher than those in the control group, and the overall satisfaction rate of postoperative analgesia in the observation group was higher than that in the control group, and the differences were statistically significant(P<0.05). There was no significant difference in the total incidence rate of postoperative complications between the two groups(P>0.05). Conclusion　Ultrasound-guided “bow tie sign” fascia iliaca block technique can achieve better analgesic effect after hip joint surgery, reduce opioid consumption, improve postoperative sleep quality of the patients, and facilitate postoperative rehabilitation of the patients.]]> 2024/8/3 12:02:39 1, WANG Hui¹, CHEN Gang², WANG Ping¹, ZHAO Shihao¹, WANG Fangqing²]]> WU Gang¹, WANG Hui¹, CHEN Gang², WANG Ping¹, ZHAO Shihao¹, WANG Fangqing² 281true ［Abstract］　Objective　To explore the application value of ultrasound in evaluating the degree of gastrointestinal wall congestion in patients with heart failure. Methods　A total of 32 patients with heart failure who were admitted to Beijing Anzhen Hospital, Capital Medical University from August 2023 to October 2023 were recruited as the heart failure group, and 33 healthy people without heart diseases during the same period were selected as the control group. The thickness of gastric wall, the thickness of the submucosa of gastric wall and the thickness of intestinal wall, inferior vena cava diameter, hepatic vein diameter, portal vein diameter and superior mesenteric vein diameter were measured by using gastrointestinal ultrasound and abdominal ultrasound in both groups. Echocardiography was performed in the heart failure group. Left ventricular ejection fraction(LVEF), right ventricular tricuspid annular plane systolic excursion(TAPSE) and pulmonary artery systolic pressure were measured. The clinical data were compared between the two groups, and the correlation of gastrointestinal wall thickness with abdominal vein diameter and cardiac function indicators in the patients of the heart failure group was analyzed. Results　The thickness of gastric wall, the thickness of the submucosa of gastric wall and the thickness of intestinal wall in the patients of the heart failure group were thicker than those in the patients of the control group, and the inferior vena cava diameter, hepatic vein diameter, portal vein diameter and superior mesenteric vein diameter in the patients of the heart failure group were wider than those in the patients of the control group, and the differences were statistically significant(P<0.05). In the patients of the heart failure group, LVEF was 45.5(25.0, 57.7)%, and TAPSE was 16.0(11.0, 18.0)mm, and pulmonary artery systolic pressure was 43.5(28.5, 59.0)mmHg. In the patients of the heart failure group, gastric wall thickness was negatively correlated with TAPSE(P<0.05) and positively correlated with portal vein diameter(P<0.05), and the thickness of the submucosa of gastric wall was negatively correlated with TAPSE(P<0.05) and positively correlated with pulmonary artery systolic pressure, inferior vena cava diameter, hepatic vein diameter and portal vein diameter(P<0.05), and intestinal wall thickness was negatively correlated with TAPSE(P<0.05) and positively correlated with inferior vena cava diameter, hepatic vein diameter and portal vein diameter(P<0.05). In the patients of the heart failure group, there was no significant correlation of the gastric wall thickness, the thickness of the submucosa of gastric wall and the intestinal wall thickness with LVEF(P>0.05). Conclusion　Ultrasound can monitor the thicknesses of the gastrointestinal wall and the submucosa of gastric wall in patients with heart failure in real time, which can be used as an imaging method to evaluate the degree of gastrointestinal wall congestion in patients with heart failure.]]> 2024/8/3 12:02:39 ZHENG Ye, QIN Huai, HAO Ruyi, ZHANG Shuang, ZHAO Qing, CHEN Jie 280true ［Abstract］　Objective　To compare the analgesic effects of ultrasound-guided intertransverse process block(ITPB) and paravertebral nerve block(PVB) on perioperative analgesia in two-port video-assisted thoracoscopic surgery. Methods　Seventy patients who underwent elective two-port thoracoscopic partial lung resection in Yancheng No.1 People＇s Hospital from November 2022 to June 2023 were recruited and divided into observation group and control group by random number table method, with 35 cases in each group. After excluding cases, 34 cases were ultimately included in each group for analysis. The observation group underwent ITPB and the control group underwent PVB. Changes in values of heart rate and mean arterial pressure before and after skin incision, intraoperative analgesic consumption, Visual Analogue Scale(VAS) scores for resting state and active state 30 minutes(T₁), 4 hours(T₂), 8 hours(T₃), 16 hours(T₄) and 24 hours(T₅) after extubation, morphine equivalent consumption, the number of analgesic pump compressions and the times of remedial analgesia 24 hours after surgery, and the occurrence of adverse reactions were compared between the two groups. Results　The nerve block time in the observation group was shorter than that in the control group, and the difference was statistically significant(P<0.05). There were no significant differences in the changes in values of heart rate and mean arterial pressure before and after skin incision, and intraoperative dosage of fentanyl and dosage of remifentanil between the two groups(P>0.05). There were no significant differences in the morphine equivalent consumption, the number of analgesic pump compressions and the times of remedial analgesia 24 hours after surgery between the two groups(P>0.05). The postoperative VAS scores for resting state and active state in the two groups increased as time progressed, but there were no significant differences in the VAS scores between the two groups at each time point(P>0.05). The incidence rate of hematoma after puncture in the observation group was significantly lower than that in the control group(P<0.05), and there were no significant differences in the incidence rates of intraoperative hypotension and postoperative nausea and vomiting between the two groups(P>0.05). Conclusion　The analgesic effect of ultrasound-guided ITPB during the perioperative period in two-port video-assisted thoracoscopic surgery is comparable to that of PVB, and the risk of hematoma after puncture in ultrasound-guided ITPB is lower and the safety is better. Ultrasound-guided ITPB can be used as an alternative therapy for PVB.]]> 2024/8/3 12:02:39 1, DU Haiyun², CHEN Qian¹, QIAN Bin^1,2]]> ZHOU Xinyue¹, DU Haiyun², CHEN Qian¹, QIAN Bin^1,2 279true ［Abstract］　Objective　To analyze the predictive value of peripheral blood CD4⁺CD25⁺ and CD8⁺CD28⁺ regulatory T cell levels for the prognosis of early cervical cancer(CC) patients after laparoscopic radical resection. Methods　A total of 204 patients with early CC who underwent laparoscopic radical resection in Danzhou People′s Hospital from September 2018 to September 2020 were enrolled. According to the prognosis of the patients during postoperative follow-up period, they were divided into poor prognosis group(43 cases) and good prognosis group(161 cases). The clinical data were compared between the two groups. Spearman rank correlation was used to analyze the correlation between peripheral blood CD4⁺CD25⁺ regulatory T cell levels and CD8⁺CD28⁺ regulatory T cell levels. Multivariate logistic regression was used to analyze the influencing factors of poor prognosis in patients with early CC after laparoscopic radical resection. Receiver operating characteristic(ROC) curve was used to evaluate the predictive value of peripheral blood CD4⁺CD25⁺ and CD8⁺CD28⁺ regulatory T cell levels for the poor prognosis of early CC patients after laparoscopic radical resection. Results　The CD4⁺CD25⁺ regulatory T cells, carcinoembryonic antigen(CEA) and carbohydrate antigen 125(CA125) levels, the proportion of positive postoperative margin and the proportion of intraoperative parametrial infiltration in the poor prognosis group were higher than those in the good prognosis group. The CD8⁺CD28⁺ regulatory T cell levels in the poor prognosis group were lower than those in the good prognosis group, and the differences were statistically significant(P<0.05). The results of Spearman rank correlation analysis showed that there was a negative correlation between peripheral blood CD4⁺CD25⁺ regulatory T cell levels and CD8⁺CD28⁺ regulatory T cell levels in early CC patients(r_s=-0.478, P<0.05). The results of multivariate logistic regression analysis showed that higher CEA, CA125 and CD4⁺CD25⁺ regulatory T cell levels were independent risk factors for promoting the occurrence of poor prognosis in the early CC patients after laparoscopic radical resection(P<0.05), and higher CD8⁺CD28⁺ regulatory T cell levels were independent protective factors for inhibiting the occurrence of poor prognosis in early CC patients after laparoscopic radical resection(P<0.05). The results of ROC curve analysis showed that peripheral blood CD4⁺CD25⁺ and CD8⁺CD28⁺ regulatory T cell levels could effectively predict poor prognosis of early CC patients after laparoscopic radical resection(P<0.05). The combination of the two indicators could further improve the predictive efficacy［AUC(95%CI)=0.939(0.898-0.979), P<0.001］, with sensitivity and specificity being 86.00% and 88.20%, respectively. Conclusion　Peripheral blood CD4⁺CD25⁺ and CD8⁺CD28⁺ regulatory T cell levels are related to the poor prognosis of early CC patients after laparoscopic radical resection, and both indicators can effectively predict the poor prognosis of early CC patients after laparoscopic radical resection.]]> 2024/8/3 12:02:39 1, HUANG Dan¹, ZHANG Jianjun², HUA Haiqin³]]> ZENG Hairong¹, HUANG Dan¹, ZHANG Jianjun², HUA Haiqin³ 278true ［Abstract］　Objective　To explore the effect of Ras homolog gene family member A(RhoA)/cofilin pathway activation on hippocampal synaptic plasticity and the mechanistic role of RhoA/cofilin pathway activation in aluminum toxicity-induced learning and memory impairments. Methods　Twenty rats were randomly selected from 30 specific pathogen-free Sprague-Dawley rats and were injected intraperitoneally with maltol-aluminum solution for 2 months to establish a rat model of chronic aluminum intoxication. The aluminum-intoxicated model rats were divided into aluminum intoxication model group(10 rats) and RhoA inhibitor group(10 rats), and the latter group was injected intraperitoneally with Rhosin hydrochloride for 30 days. The remaining 10 normal rats from the 30 specific pathogen-free SD rats were used as blank control group. The learning and memory abilities of the rats were tested by Morris water maze experiment. Transmission electron microscopy was used to observe changes in the ultrastructure of synapses in the CA1 region of the hippocampus of the rats. Real-time fluorescent quantitative polymerase chain reaction(RT-qPCR) and immunohistochemical staining were used to detect the localized expressions of RhoA, cofilin, post-synaptic density protein-95(PSD-95) and synaptophysin(SYN) in the CA1 region of the hippocampal tissues of the rats. Results　The results of Morris water maze experiment showed that the latency period of the rats in the aluminum intoxication model group was significantly longer than that in the blank control group(P<0.05), and the latency period of the rats in the RhoA inhibitor group was significantly shorter than that in the aluminum intoxication model group(P<0.05). The results of RT-qPCR showed that compared with those in the blank control group, the level of RhoA mRNA expression in CA1 region of the hippocampal tissues in the aluminum intoxication model group increased, while the levels of cofilin mRNA, PSD-95 mRNA and SYN mRNA expressions decreased, and the differences were statistically significant(P<0.05). Compared with those in the aluminum intoxication model group, the level of RhoA mRNA expression in the CA1 region of the hippocampal tissues of the rats in the RhoA inhibitor group decreased, and the levels of cofilin mRNA, PSD-95 mRNA and SYN mRNA expressions increased, and the differences were statistically significant(P<0.05). The results of immunohistochemical staining showed that compared with the blank control group, the aluminum intoxication model group had an increase in the percentage of RhoA-positive cells in the CA1 region of the hippocampus, while the aluminum intoxication model group had decreases in the percentages of cofilin-, PSD-95-, and SYN-positive cells, and the differences were statistically significant(P<0.05). Compared with the aluminum intoxication model group, the RhoA inhibitor group had a decrease in the percentage of RhoA-positive cells in the CA1 region of the hippocampus, while the RhoA inhibitor group had increases in the percentages of cofilin-, PSD-95-, and SYN-positive cells, and the differences were statistically significant(P<0.05). The results of the observations by using transmission electron microscopy revealed that compared with those in the blank control group, the number of synapses was less, and the thickness of the postsynaptic dense substance became thinner, and the width of the synaptic cleft became narrower in the aluminum intoxication model group, and the differences were statistically significant(P<0.05). The results of the observations by using transmission electron microscopy revealed that compared with those in the aluminum intoxication model group, the number of synapses was more, and the thickness of the postsynaptic dense substance became thicker, and the width of the synaptic cleft became wider in the RhoA inhibitor group, and the differences were statistically significant(P<0.05). Compared with those in the blank control group, the mitochondrial morphology in the aluminum intoxication model group changed significantly, and the mitochondria in the RhoA inhibitor group slightly dilated and the membrane structure remained intact, and the mitochondrial morphology and synaptic ultrastructure in the RhoA inhibitor group were better than those in the aluminum intoxication model group. Conclusion　Aluminum intoxication can disrupt hippocampal synaptic plasticity through the activation of the RhoA/cofilin signaling pathway, subsequently affecting learning and memory abilities.]]> 2024/8/3 12:02:39 GUO Jianxiong, LIU Wenjing, WANG Xiaoyi, CHENG Houzhi, ZHANG Lifeng, LIAO Suchan, LI Yanli, HUANG Junjie 277true ［Abstract］　Objective　To explore the short-term efficacy and safety of drug-eluting bead transarterial chemoembolization(DEB-TACE) combined with conventional transarterial chemoembolization(C-TACE) in treatment of huge hepatocellular carcinoma. Methods　The clinical data of 44 patients with huge hepatocellular carcinoma who received interventional therapy in Affiliated Hospital of Guilin Medical University and Guangxi Medical University Cancer Hospital from August 2017 to September 2020 were retrospectively analyzed. Among them, 27 patients receiving DEB-TACE combined with C-TACE treatment were selected as observation group, and 17 patients receiving DEB-TACE treatment alone were selected as control group. Modified Response Evaluation Criteria in Solid Tumors(mRECIST) were used to evaluate the short-term efficacy of the patients in the two groups. The changes of biochemical indicators before and after operation and the occurrence of postoperative adverse reactions were compared between the two groups. Results　There were no significant differences in objective response rate(ORR) and disease control rate(DCR) between the two groups 1 month and 3 months after surgery(P>0.05). The levels of total bilirubin(TBIL), alanine aminotransferase(ALT), aspartate aminotransferase(AST), prothrombin time(PT), white blood cell(WBC) and blood urea nitrogen(BUN) in both groups showed a trend of first increasing and then decreasing after operation, while the level of platelet(PLT) showed a trend of first decreasing and then increasing, and the levels of alpha fetoprotein(AFP), albumin(ALB), hemoglobin(Hb) and creatinine(CREA) showed a downward trend, and there was no statistically significant difference in the changing amplitude of each indicator between the two groups(P>0.05). There were no statistically significant differences in the incidence rates of adverse reactions such as fever, abdominal pain, nausea and vomiting, constipation and bone marrow suppression between the two groups(P>0.05). Conclusion　The short-term efficacy of DEB-TACE combined with C-TACE in treatment of huge hepatocellular carcinoma is not superior to that of DEB-TACE alone.]]> 2024/8/3 12:02:39 1, ZHAO Chang²]]> MIAO Ying¹, ZHAO Chang² 276true ［Abstract］　Objective　To analyze the cognitive function characteristics and changes in cerebral blood flow in young and middle-aged patients with mild cognitive impairment(MCI) and white matter hyperintensities(WMH). Methods　A total of 70 WMH patients, aged 35-65 years, admitted to Department of Neurology, Affiliated Hospital of Yangzhou University from February 2023 to December 2023 were recruited. Neuropsychological scale score, brain magnetic resonance imaging(MRI) and transcranial Doppler(TCD) examinations were performed in all the patients, and the patients′ general clinical data were collected. According to the Beijing Version of Montreal Cognitive Assessment(MoCA) scores, the study subjects were divided into MCI group(with MoCA scores of 20-24 points, 36 cases) and control group(with MoCA scores of 25-30 points, 34 cases). The clinical baseline data, cognitive subdomain scores and TCD indicators were compared between two groups. Logistic regression analysis was used to explore the correlation between pulsatility index(PI) of middle cerebral artery(MCA) and the occurrence of MCI. Results　The visual space and execute function, naming, attention, language, abstract thinking and delayed memory ability of MoCA in the control group were significantly better than those in the MCI group(P<0.05). The long delayed recall scores and total scores of Auditory Verbal Learning Test-Huashan Version(AVLT-H) in the MCI group were lower than those in the control group, and the differences were statistically significant(P<0.05). The scores of Verbal Fluency Test(VFT), Symbol Digit Modalities Test(SDMT) and Clock Drawing Test(CDT) in the MCI group were lower than those in the control group. The duration of Trail Making Test A(TMT-A) and Trail Making Test B(TMT-B) in the MCI group was longer than that in the control group, and the difference was statistically significant(P<0.05). The PI level of MCA in the MCI group was higher than that in the control group, and the difference was statistically significant(P<0.05). There were no statistically significant differences in the other TCD indicators(P>0.05). After adjusting for gender, history of hypertension, history of diabetes, history of hyperlipidemia, history of smoking and history of alcohol drinking, the results of logistic regression analysis showed that the increased PI level of MCA was a risk factor for promoting the occurrence of MCI［OR(95%CI)=348.877(2.046-59 419.483), P=0.026］. Conclusion　The cognitive subdomain functions of language, visual space, memory, execution, attention and processing speed in young and middle-aged MCI patients with WMH are all reduced. The elevated PI level of MCA helps to identify the high-risk population.]]> 2024/7/2 0:00:00 1,2, ZHANG Yu¹, YANG Suxin^1,2, ZHANG Teng^1,2, ZHAO Li^1,2, TANG Tieyu¹, XU Xiaotian¹]]> YAN Yidan^1,2, ZHANG Yu¹, YANG Suxin^1,2, ZHANG Teng^1,2, ZHAO Li^1,2, TANG Tieyu¹, XU Xiaotian¹ 275true ［Abstract］　Objective　To study the mechanism of action of Ziyuglycoside Ⅰ on knee osteoarthritis in rats. Methods　Eight male Sprague-Dawley(SD) suckling rats were selected to extract chondrocytes, and the cell proliferation rate was detected by CCK-8 assay to determine the optimal drug concentration. The chondrocytes were randomly divided into control group, inflammation group and treatment group. The control group was cultured in complete medium, and the inflammation group was cultured in medium containing 10 μg/mL interleukin-1β(IL-1β), and the treatment group was cultured in medium containing 10 μg/mL IL-1β and the optimal concentration of Ziyuglycoside Ⅰ . Calcein-AM/PI staining was used to observe the growth of chondrocytes in each group. Real-time fluorescent quantitative polymerase chain reaction(RT-qPCR) was used to detect the relative expressions of interleukin-6(IL-6) and matrix metalloproteinase 13(MMP13) mRNA in chondrocytes in each group. Twenty-four male SD rats about 8 weeks old were randomly divided into control group, inflammation group and treatment group, with 8 rats in each group. Osteoarthritis models were established for the inflammation group and the treatment group. After 4 weeks of completing the osteoarthritis models, the inflammation group and the control group were injected with equal volume of normal saline into the joint cavity, and the treatment group was injected with the optimal concentration of Ziyuglycoside Ⅰ solution into the joint cavity, and the drugs were administered once a week for 4 weeks. The tissue structure of the knee joints in each group was observed by using hematoxylin and eosin(HE) staining and safranine O-solid green staining. Immunohistochemical staining was used to observe the expressions of IL-6 and MMP13 proteins in the knee joint tissues of each group. Results　The results of CCK-8 assay showed that no obvious cytotoxicity was observed in all the experimental concentrations, and the chondrocyte proliferation rate was the highest when the concentration of Ziyuglycoside Ⅰ was 20 mg/L. The results of Calcein-AM/PI staining showed that the cell proliferation rate in the inflammation group and the treatment group was lower than that in the control group, and the cell proliferation rate in the treatment group was higher than that in the inflammation group, and the differences were statistically significant(P<0.05). The results of RT-qPCR detection showed that the relative expression levels of IL-6 and MMP13 mRNA in the inflammation group and the treatment group were higher than those in the control group, and the relative expression levels of IL-6 and MMP13 mRNA in the treatment group were lower than those in the inflammation group, and the differences were statistically significant(P<0.05). The results of HE staining and safranine O-solid green staining showed that the tissue structure of the knee joints in the control group was more complete than that in the inflammation group and the treatment group, and compared with the inflammation group, the treatment group showed a reduction in knee joint tissue damage. The results of immunohistochemical staining showed that the inflammation group had the highest expression levels of IL-6 and MMP13 proteins and the treatment group had higher expression levels of IL-6 and MMP13 proteins than the control group. Conclusion　Ziyuglycoside Ⅰ can play a role in anti-inflammatory effects by maintaining chondrocyte vitality, reducing the release of inflammatory factors and reducing the degradation of extracellular matrix(ECM) of chondrocytes, and has certain medicinal potential in treatment of knee osteoarthritis.]]> 2024/7/2 0:00:00 1, ZHOU Anyuan¹,WANG Yonglin¹, PEI Xiaomin¹, ZHONG Qiuling¹, YANG Yuan^1,2]]> WANG Zhewei¹, ZHOU Anyuan¹,WANG Yonglin¹, PEI Xiaomin¹, ZHONG Qiuling¹, YANG Yuan^1,2 274true ［Abstract］　Objective　To explore the relationship between serum tumor necrosis factor-related apoptosis-inducing ligand(TRAIL) level and immunotherapy response in patients with advanced non-small cell lung cancer(NSCLC). Methods　The clinical data of 70 patients with advanced NSCLC who were admitted to Tangshan People′s Hospital from January 2019 to May 2020 were retrospectively analyzed. The serum TRAIL level was determined by using enzyme-linked immunosorbent assay(ELISA) within 24 hours before immunotherapy. Immunotherapy response was assessed by using objective response rate(ORR) and clinical benefit rate(CBR). The relationship of the patients′ serum TRAIL levels with immunotherapy response, lung function, emphysema and clinical prognosis was analyzed. Results　After immunotherapy, 23 patients with NSCLC achieved objective remission and 46 patients achieved clinical benefit. The serum TRAIL level in patients with objective remission was significantly higher than that in patients without objective remission［27.77(23.13, 39.13)pg/mL vs 12.36(8.76, 18.15)pg/mL; Z=-4.508, P<0.001］. The serum TRAIL level in patients with clinical benefit was significantly higher than that in patients without clinical benefit［23.13(16.99, 30.63)pg/mL vs 11.75(8.76, 15.56)pg/mL; Z=-4.887, P<0.001］. The results of Spearman rank correlation analysis showed that serum TRAIL level was positively correlated with forced expiratory volume in 1 second(FEV₁)/forced vital capacity(FVC)(r_s=0.288, P=0.016), and negatively correlated with total emphysema ratio(r_s=-0.257, P=0.032) and lobar emphysema rate(LER)(r_s=-0.324, P=0.006). The results of multivariate logistic regression analysis showed that taking serum TRAIL level >27.46 pg/mL as a reference, patients with serum TRAIL level <18.15 pg/mL had a significantly increased risk of not achieving objective remission and clinical benefit after immunotherapy(P<0.05). The results of receiver operating characteristic(ROC) curve analysis showed that serum TRAIL level could effectively predict the response of NSCLC patients to immunotherapy(P<0.05). The overall survival(OS) and progression-free survival(PFS) prognoses of the high TRAIL level group(serum TRAIL≥18.15 pg/mL) were significantly better than those of the low TRAIL level group(serum TRAIL<18.15 pg/mL)(P<0.05). Conclusion　Low serum TRAIL level is associated with non-response to immunotherapy and poor clinical prognosis in advanced NSCLC patients, and monitoring this indicator helps to screen NSCLC patients who can benefit from immunotherapy.]]> 2024/7/2 12:19:14 1, ZHANG Zhen′an¹, WANG Jihua², ZHOU Yuan¹, LIANG Fengxia¹]]> ZHANG Yajun¹, ZHANG Zhen′an¹, WANG Jihua², ZHOU Yuan¹, LIANG Fengxia¹ 273true ［Abstract］　Objective　To analyze the effects and treatment costs of different progesterone administration regimens on patients undergoing preimplantation genetic testing for frozen-thawed embryo transfer(PGT-FET). Methods　The clinical data of 169 infertile patients who underwent PGT-FET in the Reproductive Medicine Center of Taihe Hospital, Shiyan City from August 2020 to July 2022 were retrospectively analyzed. According to the progesterone administration regimens adopted by the patients, the patients were divided into group A(oral administration of progesterone capsules, 82 cases) and group B(vaginal administration of progesterone softgel capsules, 87 cases). The hormonal indicators and oocyte harvest on the day of injection of human chorionic gonadotropin(HCG), endometrial thickness and receptivity indicators［resistance index(RI), pulse index(PI) and peak systolic velocity/end diastolic velocity(S/D)］ on the day of injection of HCG and on the day of embryo transfer, embryo transfer, pregnancy outcomes, vaginal bleeding and cost-effectiveness ratio were compared between the two groups. Results　There were no significant differences between the two groups in luteinizing hormone(LH) on the day of injection of HCG, estradiol(E₂) on the day of injection of HCG, progesterone(P) on the day of injection of HCG, number of oocyte harvest, number of MⅡoocytes, and endometrial thickness and uterine artery S/D, RI, PI on the day of injection of HCG and on the day of embryo transfer, the number of transferred blastocysts, the rate of high-quality transferred blastocysts, biochemical pregnancy rate, clinical pregnancy rate, first trimester abortion rate and continue pregnancy rate(P>0.05). The rate of early vaginal bleeding and the rate of late vaginal bleeding in the group A were significantly lower than those in the group B(P<0.05). The cost-effectiveness ratio of the group A(6.72) was lower than that of the group B(8.41). Conclusion　In PGT-FET patients, the clinical effect of oral administration of progesterone capsules and the protective effect of oral administration of progesterone capsules on endometrial receptivity are similar to those of vaginal administration of progesterone softgel capsules, but the former treatment can reduce the rate of vaginal bleeding and treatment costs.]]> 2024/7/2 0:00:00 1, ZHAO Lei¹, ZHAO Yu², LIU Qianru³]]> HE Chenhui¹, ZHAO Lei¹, ZHAO Yu², LIU Qianru³ 272true ［Abstract］　Objective　To explore the effects of virtual reality(VR) technology-based brain visual training on visual function defects and visual quality in glaucoma patients. Methods　A total of 98 glaucoma patients treated in 363 Hospital from March 2021 to February 2023 were selected and divided into observation group and control group according to random number table method, with 49 cases in each group. The control group was given routine visual function training, and the observation group was given VR technology-based brain visual training on the basis of the control group. Both groups were continuously trained for 3 months. The visual acuity, intraocular pressure, visual function defects, retinal nerve fiber layer(RNFL) thickness, optic disc volume(DV), National Eye Institute Visual Functioning Questionnaire-25(NEI VFQ-25) score, Glaucoma Quality of Life-15(GQL-15) score were compared between the two groups. Results　After 1 month and 3 months of training, the visual acuity, RNFL thickness, DV and NEI VFQ-25 scores in the observation group were higher than those in the control group, while the intraocular pressure and GQL-15 scores in the observation group were lower than those in the control group, and the differences were statistically significant(P<0.05). Before training and 1 month after training, there were no statistically significant differences in the defect rates of inhibition, perceptual eye position, stereoscopic vision, fixation stability and visual noise between the two groups(P>0.05). After 3 months of training, the defect rates of inhibition, perceptual eye position, stereoscopic vision, fixation stability and visual noise in the observation group were significantly lower than those in the control group(P<0.05). Conclusion　VR technology-based brain visual training can improve the visual acuity, visual function, RNFL thickness and DV of glaucoma patients, and reduce their intraocular pressure, and improve their visual quality and quality of life.]]> 2024/7/2 12:19:14 ZHOU Yu, LI Man, FENG Yi 271true ［Abstract］　Objective　To compare the efficacy of bilateral thoracic sympathectomy at different segments of T₃ and T₄ under thoracoscopy for treatment of primary palmar hyperhidrosis(PPH). Methods　The clinical data of 125 patients with PPH who underwent thoracic sympathectomy under thoracoscopy in the Department of Thoracic Surgery of Xuanwu Hospital Capital Medical University from January 2017 to March 2023 were retrospectively collected. The patients were divided into T₃ group(60 cases) and T₄ group(65 cases) according to thoracic sympathectomy at different segments. The T₃ group underwent bilateral thoracic sympathectomy at segment of T₃ under thoracoscopy, and the T₄ group underwent bilateral thoracic sympathectomy at segment of T₄ under thoracoscopy. The clinical data, the recurrence rate of PPH and the occurrence of compensatory hyperhidrosis(CH) at different time points after the operation, and the patients′ satisfaction with the operation at 12 months after the operation were compared between the two groups. Results　There were no statistically significant differences in age, gender, family history, duration of symptoms, PPH grade, duration of surgery, postoperative complications and length of hospital stay between the two groups(P>0.05), and the surgical effectiveness rate was 100% in both groups. At 1 month, 6 months and 12 months after surgery, there were no significant differences in the recurrence rates of PPH between the two groups(P>0.05). At 1 month after surgery, there were no significant differences in the incidence of CH and the severity of CH between the two groups(P>0.05). At 6 months and 12 months after surgery, the incidence of CH in the T₃ group was significantly higher than that in the T₄ group(P<0.05), and there was significant difference in the severity of CH between the two groups(P<0.05). At 12 months after surgery, there was no significant difference in the patients′ satisfaction with the operation between the two groups(P>0.05). Conclusion　Both bilateral thoracic sympathectomy at segment of T₃ under thoracoscopy and bilateral thoracic sympathectomy at segment of T₄ under thoracoscopy have good efficacy in the treatment of PPH. Compared with bilateral thoracic sympathectomy at segment of T₄, bilateral thoracic sympathectomy at segment of T₃ results in higher incidence rates of CH at 6 months and 12 months after surgery.]]> 2024/7/2 12:19:14 WANG Tengteng, ZHI Xiuyi, LIU Baodong, ZHANG Peilong 270true ［Abstract］　Objective　To systematically evaluate the efficacy of acupoint therapy for endometriosis(EMT). Methods　The literature related to the treatment of EMT with different Traditional Chinese Medicine(TCM) external therapeutic methods represented by acupoint therapy was searched in PubMed, the Cochrane Library, Embase, and Chinese National Knowledge Infrastructure(CNKI) databases. The searched papers were collected in each database from its establishment time to June 1, 2023. Finally, 66 papers were included, all of which were randomized controlled trial(RCT) studies, including 6 therapeutic combinations: simple TCM therapy, simple Western medicine therapy, simple acupoint therapy, TCM combined with acupoint therapy, Western medicine combined with acupoint therapy, Western medicine and TCM combined with acupoint therapy. Network Meta-analysis was used to evaluate the effects of the therapeutic methods on major outcome indicators including cancer antigen 125(CA125), estradiol(E₂), follicle-stimulating hormone(FSH), luteinizing hormone(LH), prostaglandin F_2α(PGF_2α) and Visual Analogue Scale(VAS) score. Results　The results of the consistency model analysis showed that in terms of the improvement of CA125 level, the effect of Western medicine combined with acupoint therapy was significantly better than that of simple TCM therapy and simple Western medicine therapy(P<0.05), and there was no statistically significant difference in the improvement of CA125 level among the other therapeutic groups(P>0.05). In terms of the improvement of LH level, the effect of Western medicine and TCM combined with acupoint therapy was significantly better than that of simple Western medicine therapy(P<0.05), and there was no statistically significant difference in the improvement of LH level among the other therapeutic groups(P>0.05). In terms of the improvement of PGF_2α level, the effect of Western medicine combined with acupoint therapy was significantly better than that of simple TCM therapy, simple Western medicine therapy, simple acupoint therapy, TCM combined with acupoint therapy, and Western medicine and TCM combined with acupoint therapy(P>0.05), and the effect of TCM combined with acupoint therapy and Western medicine and TCM combined with acupoint therapy was significantly better than that of simple Western medicine therapy(P<0.05). There was no statistically significant difference in the improvement of PGF_2α level among the other therapeutic groups(P>0.05). There were no statistically significant differences in the improvements of E₂ and FSH levels, and VAS scores among the other therapeutic groups(P>0.05). Conclusion　Acupoint therapy, as an adjuvant therapy, can effectively promote the improvement of key indicators of EMT.]]> 2024/7/2 12:19:14 1, HE Shenglan², DU Zifei³, LYU Hang⁴, WANG Yong³]]> YUAN Xiaoyi¹, HE Shenglan², DU Zifei³, LYU Hang⁴, WANG Yong³ 269true ［Abstract］　Objective　To compare the differences in responses to laparoscopic surgery under intubation general anesthesia among patients with different constitutions of Traditional Chinese Medicine. Methods　One hundred and nineteen patients who underwent laparoscopic abdominal surgery under intubation general anesthesia in Nanning Hospital of Traditional Chinese Medicine from January 2019 to September 2022 were recruited. According to the Classification and Judgment of Traditional Chinese Medicine Constitutions(ZYYXH-T157-2009), the patients were divided into gentle constitution group(50 cases), Yang deficiency constitution group(36 cases) and Qi deficiency constitution group(33 cases). The mean arterial pressure, heart rate, propofol dosage during anesthesia maintenance and the incidence of adverse reactions were compared among the three groups 5 minutes before anesthesia induction(T₁), at intubation(T₂), and 5 minutes after intubation(T₃). Results　From the time point of T₁ to the time point of T₃, the mean arterial pressure and heart rate showed a downward trend in the three groups. At the time point of T₂, the mean arterial pressure in the Yang deficiency constitution group was significantly higher than that in the gentle constitution group and the Qi deficiency constitution group(P<0.05). There was no significant difference in the mean arterial pressure among the three groups at the time point of T₁ and the time point of T₃(P>0.05). There was no significant difference in the heart rate among the three groups at the time point of T₁ to the time point of T₃(P>0.05). The propofol dosage in the gentle constitution group and the Yang deficiency constitution group was significantly higher than that in the Qi deficiency constitution group(P<0.05), but there was no statistically significant difference between the gentle constitution group and the Yang deficiency constitution group(P=0.100). The incidence of hypotension in the Qi deficiency constitution group was significantly higher than that in the gentle constitution group and the Yang deficiency constitution group(P<0.05). The incidence of nausea and vomiting in the gentle constitution group was significantly lower than that in the Yang deficiency constitution group and the Qi deficiency constitution group(P<0.05). There was no significant difference in the incidence of bradycardia among the three groups(P>0.05). Conclusion　Patients with different constitutions of Traditional Chinese Medicine have different responses to laparoscopic surgery under intubation general anesthesia, and clinicians should conduct classified management for the patients.]]> 2024/7/2 12:19:14 TANG Xiaoxiao, PENG Li, ZHANG Yongchao 268true ［Abstract］　Objective　To explore the effect of continuous positive airway pressure(CPAP) ventilation on respiratory function in patients undergoing thoracoscopic lung surgery. Methods　A total of 60 patients who underwent elective thoracoscopic lung surgery in the People′s Hospital of Guangxi Zhuang Autonomous Region from June 2021 to December 2022 were selected and divided into observation group and control group by random number table method, with 30 cases in each group. After exclusions, 29 cases were included in each of the two groups for analysis. During one-lung ventilation, the observation group received CPAP ventilation on the surgical side of the lung, with a pressure of 5 cmH₂O, while the control group received no special intervention measures on the surgical side of the lung. Arterial blood gas analysis was performed at the time point of two-lung ventilation for 20 minutes(T₀), the time point of one-lung ventilation for 30 minutes(T₁) and the time point of one-lung ventilation for 1 hour(T₂), and oxygenation index(OI) level was compared between the two groups at each time point. The neutrophil ratio and white blood cell count were compared between the two groups before operation and 24 hours after operation. The incidence of pulmonary complications within 72 hours after operation was compared between the two groups. Results　At the time points from T₀ to T₂, OI in both groups showed a downward trend(P<0.05), and at the time points of T₁ and T₂, OI levels in the control group were lower than those in the observation group, and the differences were statistically significant(P<0.05). After operation, the white blood cell count and the neutrophil ratio in the two groups were significantly higher than those before operation(P<0.05), and there were no statistically significant differences between the two groups(P>0.05). The incidence of postoperative pulmonary complications in the observation group was lower than that in the control group, and the difference was statistically significant(13.79% vs 37.93%; χ²=0.033=4.406, P=0.036). Conclusion　The implementation of CPAP ventilation in the surgical side of the lung in the patients undergoing thoracoscopic lung surgery can effectively improve the oxygenation of the patients with one-lung ventilation and reduce the incidence of postoperative pulmonary complications.]]> 2024/7/2 12:19:14 HUANG Yuting, YU Meigang, MO Yijie, MA Li 267true ［Abstract］　Objective　To compare the effects of dexmedetomidine and propofol on sedation in patients with short-term mechanical ventilation after elective surgery. Methods　A total of 97 patients who were returned to the intensive care unit(ICU) with tracheal intubation after surgery under general anesthesia in Peking University International Hospital from April 1, 2022 to November 30, 2023 were recruited. All the patients underwent short-term(<24 hours) tracheal intubation mechanical ventilation, continuous sedation and analgesia. The patients were divided into observation group(49 cases, intervention with dexmedetomidine) and control group(48 cases, intervention with propofol) by using random number table method. The use of antihypertensive drugs(nicardipine hydrochloride) during ICU observation was recorded in the two groups. The vital signs indicators［heart rate(HR), respiratory rate(RR), mean arterial pressure(MAP)］, plasma catecholamine［epinephrine(E), norepinephrine(NE)］ levels before sedation(T₀), 1 hour after sedation(T₁) and 2 hours after tracheal extubation(T₂) between the two groups and between the subgroups using antihypertensive drugs, and the incidence of delirium was recorded in the two groups. Results　At the time points from T₀ to T₂, HR and MAP showed a downward trend and then an upward trend, and RR showed a stable trend and then an upward trend, and E showed an upward trend and then a downward trend and NE showed a continuous upward trend in the two groups, and there was statistically significant difference in the changing amplitude of NE between the two groups(P<0.05), and at the time point of T₂, the NE level in the observation group was significantly lower than that in the control group(P<0.05). During ICU period, there were 2 cases of delirium in the observation group and 2 cases in the control group, and there was no significant difference in the incidence of delirium between the two groups(4.08% vs 4.17%; P=1.000). There were 12 patients in the observation group and 11 patients in the control group who used antihypertensive drugs during ICU period, and there was no statistically significant difference in the utilization rate of antihypertensive drugs between the two groups(24.49% vs 22.92%; χ²=0.033, P=0.885). For the patients using antihypertensive drugs, there were no significant differences in the duration of antihypertensive medication use and the total dose of antihypertensive drugs between the two groups(P>0.05). Among the patients using antihypertensive drugs, E in the observation group showed an upward trend and then a downward trend, while E in the control group showed an upward trend, and there was statistically significant difference in the changing amplitude of NE between the two subgroups(P<0.05), and at the time point of T₂, the NE level of the observation group was significantly lower than that of the control group(P<0.05). In the patients who did not use antihypertensive drugs, the levels of HR, MAP and NE at the time point of T₂ in the observation group were lower than those in the control group, and the differences were statistically significant between the two groups(P<0.05). Conclusion　The use of dexmedetomidine for sedation results in less blood pressure fluctuations, lower plasma catecholamine levels and stronger inhibition of the sympathetic nervous system in patients before and after extubation.]]> 2024/7/2 12:19:14 1,2, YUAN Hongxun², ZHAO Huiying¹, WANG Zheng², AN Youzhong¹]]> QIAO Li^1,2, YUAN Hongxun², ZHAO Huiying¹, WANG Zheng², AN Youzhong¹ 266true ［Abstract］　Objective　To explore the expression of miR-145-5p in colorectal cancer and its regulatory effect on guanosine triphosphate(GTP)-binding protein 2(GTPBP2). Methods　The cancer tissues and cancer-adjacent tissues of 44 patients with colorectal cancer who underwent radical resection of colorectal cancer in Shangqiu First People′s Hospital, Xinxiang Medical College from January 2022 to December 2022 were selected. Normal human colorectal mucosa cell FHC and colorectal cancer cell HCT116 were selected for basic experiments. Fluorescent quantitative polymerase chain reaction(qPCR) was used to detect the expression levels of miR-145-5p and GTPBP2 mRNA in cancer tissues, cancer-adjacent tissues and the cells. Western blot method was used to detect the expression level of GTPBP2 protein in the cells. Dual luciferase reporter gene assay was used to verify the targeting relationship between miR-145-5p and GTPBP2 gene. The correlation between the expression level of miR-145-5p in colorectal cancer tissues and the clinicopathological features of the patients was analyzed. Results　Compared with that in the cancer-adjacent tissues, the expression level of miR-145-5p in cancer tissues was significantly decreased(t=8.189, P<0.001), and the mRNA expression level of GTPBP2 was significantly increased(t=11.230, P<0.001). The results of Pearson correlation analysis showed that the expression level of miR-145-5p in colorectal cancer tissues was negatively correlated with the mRNA expression level of GTPBP2(r=-0.503, P<0.001). The expression level of miR-145-5p was significantly associated with distant metastasis, lymph node metastasis and TNM stage of colorectal cancer(P<0.05), but was not significantly associated with the patients′ gender, age, lesion location, clinical T stage and tumor diameter(P>0.05). Compared with that in the FHC cells, the expression level of miR-145-5p in the HCT116 cells was significantly decreased(P<0.001), and the mRNA and protein expression levels of GTPBP2 were significantly increased(P<0.001). Overexpression of miR-145-5p could down-regulate the expressions of GTPBP2 mRNA and protein in the HCT116 cells. The results of dual luciferase reporter gene assay suggested a targeting relationship between miR-145-5p and GTPBP2 gene. Conclusion　The expression of miR-145-5p is low in colorectal cancer and miR-145-5p is significantly associated with distant metastasis, lymph node metastasis, and TNM stage and it may affect the occurrence and development of colorectal cancer through targeted regulation of GTPBP2 expression.]]> 2024/5/31 0:00:00 WANG Yongfu, ZHENG Ziheng, XU Chunjin 265true ［Abstract］　Objective　To explore the effect of ratio of follicles in diameter of 16-18 mm on in vitro fertilization and embryo transfer(IVF-ET) outcomes in polycystic ovary syndrome(PCOS) patients receiving antagonist protocols on human chorionic gonadotropin(HCG) trigger day in antagonist protocols. Methods　The clinical data of PCOS patients undergoing IVF-ET assisted pregnancy in Women′s Hospital of Nanjing Medical University from January 2016 to December 2021 were retrospectively analyzed. The patients were divided into three groups(group A, group B and group C) according to the ratio of follicles in diameter of 16-18 mm in bilateral ovaries on the HCG trigger day using a tertile method. Group A had a ratio <0.43(262 cases), and group B had a ratio between 0.43 and 0.56(267 cases), and group C had a ratio ≥0.56(247 cases). The general clinical data, ovulation induction cycles, embryo development, first frozen-thawed embryo transfer(FET) outcomes after IVF-ET cycle, and pregnancy and perinatal outcomes were compared among the three groups. Results　The values of baseline luteinizing hormone(LH) and baseline LH to baseline follicle-stimulating hormone(FSH) ratio in group B were significantly higher than those in group A and group C(P<0.05), and the differences were statistically significant(P<0.05). The total dose of gonadotropin(Gn) in group A was higher than that in group B and group C, and estradiol(E₂) level on the HCG trigger day in group A was higher than that in group B and group C, and the differences were statistically significant(P<0.05). The number of retrieved oocytes, 2-pronucleus(PN) embryos, cleavage-stage embryos, and blastocysts, as well as the number of transferable embryos in group B and group C were higher than those in group A, and the differences were statistically significant(P<0.05). The rate of blastocyst formation in group C was higher than that in group A and group B, and the rate of blastocyst formation with high scores in group C was higher than that in group B, and the differences were statistically significant(P<0.05). There were no significant differences among the three groups in terms of the number of transferred embryo, the days of transferred embryos, endometrial preparation protocols and endometrial thickness(P>0.05). There were no significant differences among the three groups in embryo implantation rate, biochemical pregnancy rate, clinical pregnancy rate, miscarriage rate, live birth rate, gestational diabetes mellitus incidence and gestational hypertension incidence(P>0.05). Conclusion　In the antagonist protocols, the ratio of follicles in diameter of 16-18 mm on HCG trigger day can reflect the developmental potential of oocytes. When the ratio is greater than or equal to 0.56, better retrieved oocytes, 2PN embryos, cleavage-stage embryos, blastocyst formation and transferable embryos are achieved, serving as a reference indicator for HCG trigger timing in PCOS patients to a certain extent. However, the ratio does not affect the subsequent first FET pregnancy outcome.]]> 2024/5/31 12:04:37 PENG Lijun, XIE Qijun, YANG Ye, WANG Xinyu, HU Qingyun, LING Xiufeng 264true ［Abstract］　Objective　To explore the heterogeneity of human umbilical cord mesenchymal stem cells(hUCMSCs) and to analyse the subpopulation based on single-cell ribonucleic acid(RNA) sequencing results. Methods　Two strains of hUCMSCs(named hUCMSCs-1 and hUCMSCs-2, respectively) presented by Zhengzhou Aobo Cell Medicine Laboratory Co., Ltd were selected, and the stemness genes［sex-determining region Y-box 2(Sox2) and Nanog］ were detected by real-time fluorescence quantitative polymerase chain reaction(qPCR). The two strains of hUCMSCs were induced into osteoblasts. After 3 days, the expression of osteogenic gene alkaline phosphatase(Alp) was detected. After 7 days, alkaline phosphatase staining was performed, and after 14 days, alizarin red staining was performed. The two strains of hUCMSCs were induced into chondrocytes, and the expression of chondrogenic gene collagen type Ⅱ alpha 1(COL2A1) was detected 21 days later. Rat chondrocytes were stimulated with 10 ng/mL interleukin-1β(IL-1β) for 24 hours. After co-culture with hUCMSCs-1 and hUCMSCs-2, the expressions of anabolic gene aggrecan(ACAN) and catabolic gene matrix metalloproteinase 3(MMP3) in the cartilage cells were detected. Single-cell RNA sequencing of hUCMSCs-1 was performed, and subpopulations were grouped according to their function. Bioinformatics analysis methods were used to depict heat maps of marker genes and enriched signaling pathways of each subpopulation. Results　There were significant differences in the levels of Sox2, Nanog, Alp and COL2A1 expressions between the two strains of hUCMSCs(P<0.05). After 7 days of osteogenic induction, the alkaline phosphatase staining of hUCMSCs-1 was darker than that of hUCMSCs-2. After 14 days of osteogenic induction, the results of alizarin red staining showed that the number of calcium nodules in hUCMSCs-1 was more than that in hUCMSCs-2. After the intervention of IL-1β, there were significant differences in MMP3 expression levels between the cartilage cells co-cultured with the two strains of hUCMSCs(P<0.05), but there was no significant difference in ACAN expression(P>0.05). Based on the results of single-cell RNA sequencing, hUCMSCs-1 could be divided into three functional subpopulations: C1, C2 and C3. The signal pathway of C1 subpopulation was enriched in the interaction of extracellular matrix receptors, which was related to the anabolism of chondrocytes. The signal pathway of C2 subpopulation was enriched in cell senescence and apoptosis, which was related to cell self-renewal. The signal pathway of C3 subpopulation was enriched in DNA replication and meiosis, which was related to the cell cycle. Conclusion　Heterogeneity exists in hUCMSCs coming from different individual sources and these hUCMSCs may differ in their osteogenic, chondrogenic, and anti-catabolic abilities. Single-cell RNA sequencing can better classify hUCMSCs according to their functional characteristics, which helps to improve the clinical efficacy of mesenchymal stem cell therapy.]]> 2024/5/31 12:04:37 1,2, QI Yuxin^1,2, BI Yifei³, ZHANG Bingjun², DAI Liming², ZHANG Xiaoling^1,2]]> LIU Kai^1,2, QI Yuxin^1,2, BI Yifei³, ZHANG Bingjun², DAI Liming², ZHANG Xiaoling^1,2 263true ［Abstract］　Objective　To analyse the expressions of YTH domain N6-methyladenosine RNA binding protein F1(YTHDF1) and eukaryotic initiation factor 3C(EIF3C) in epithelial ovarian cancer(EOC) and their clinical significance. Methods　The clinical data of 130 patients who were itially diagnosed with EOC and treated in the Affiliated Hospital of Xuzhou Medical University from January 2016 to January 2020 were retrospectively analyzed, and the normal ovarian tissues of 70 patients who underwent surgical treatment for benign ovarian cysts during the same period were selected as controls. Real-time quantitative polymerase chain reaction(RT-qPCR) was used to detect the levels of YTHDF1 mRNA and EIF3C mRNA expressions in the tissues. Immunohistochemical staining was used to detect the expressions of YTHDF1 protein and EIF3C protein in the tissues. The correlation between the levels of YTHDF1 mRNA and EIF3C mRNA expressions was explore by using Pearson correlation analysis. The correlation between the expressions of YTHDF1 protein and EIF3C protein and the clinicopathological characteristics of EOC patients were analyzed. Kaplan-Meier method was used to plot survival curve. Cox regression was used to analyze the influencing factors of prognosis in the EOC patients. Results　The levels of YTHDF1 mRNA and EIF3C mRNA expressions in the EOC tissues were higher than those in the normal ovarian tissues, and the differences were statistically significant(P<0.05). The positive rates of YTHDF1 protein and EIF3C protein expressions in the EOC tissues were higher than those in the normal ovarian tissues, and the differences were statistically significant(P<0.05). The results of Pearson correlation analysis showed that the expression of YTHDF1 mRNA was positively correlated with that of EIF3C mRNA in the EOC tissues(r=0.802, P<0.001). The expressions of YTHDF1 protein and EIF3C protein in the EOC tissues were significantly correlated with International Federation of Gynecology and Obstetrics(FIGO) stage, pathological grade and lymph node metastasis(P<0.05). The survival prognosis of the negative YTHDF1 patients was better than that of the positive YTHDF1 patients(log rank test: χ²=6.120, P=0.013). The survival prognosis of the negative EIF3C patients was better than that of the positive EIF3C patients(log rank test: χ²=10.610, P=0.001). The results of Cox regression analysis showed that FIGO stage Ⅲ, pathological grade Ⅲ, lymph node metastasis, higher levels of carbohydrate antigen 125(CA125), and positive expressions of YTHDF1 protein and EIF3C protein were independent risk factors for poor prognosis of the EOC patients. Conclusion　The expressions of YTHDF1 and EIF3C are elevated in the EOC tissues, which is associated with adverse clinicopathological characteristics. YTHDF1 and EIF3C are potential biomarkers for evaluating the prognosis of EOC.]]> 2024/5/31 12:04:37 1, WANG Guanghui², CHEN Ting³, ZHANG Jia³, ZHAO Na³]]> FENG Shixiang¹, WANG Guanghui², CHEN Ting³, ZHANG Jia³, ZHAO Na³ 262true ［Abstract］　Objective　To analyze the the efficacy of fibrinogen degradation products(FDP) and D-dimer(D-D) combined with platelet(PLT) in predicting survival prognosis of patients with trauma-induced coagulopathy(TIC). Methods　The clinical data of 182 trauma patients admitted to the First Hospital of Kunming from February 2020 to February 2023 were retrospectively collected. According to the occurrence of TIC, the patients were divided into TIC group(85 cases) and non-TIC group(97 cases). The clinical data were compared between the two groups, and the effects of FDP, D-D and PLT levels in the TIC patients at admission on the occurrence of death within 30 days after admission were explored by using multivariate logistic regression analysis, and the predictive efficacy of the above indicators was analyzed by using receiver operating characteristic(ROC) curve. Results　The levels of injury severity score(ISS), prothrombin time(PT), activated partial thromboplastin time(APTT), prothrombin time ratio(PTR), international normalized ratio(INR) and death rate in the TIC group were higher than those in the non-TIC group, while the levels of fibrinogen(Fib), PLT and hemoglobin(Hb) in the TIC group were lower than those in the non-TIC group, and the differences were statistically significant(P<0.05). Among the TIC patients, 59 cases survived and 26 cases died within 30 days after admission. The FDP and D-D levels in the survival group were significantly lower than those in the death group(P<0.05), and the PLT level in the survival group was significantly higher than that in the death group(P<0.05). There was no statistically significant difference in Fib level between the two groups(P>0.05). After adjusting the factors of age, gender, length of hospital stay and ISS, the results of multivariate logistic regression analysis showed that the elevated levels of FDP［OR(95%CI)=1.021(1.007-1.036)］ and D-D［OR(95%CI)=1.087(1.027-1.250)］ were risk factors for promoting death in the patients(P<0.05), and the elevated level of PLT［OR(95%CI)=0.990(0.983-0.997)］ was a protective factor for inhibiting death in the patients(P<0.05). The results of ROC curve analysis showed that FDP, D-D and PLT could effectively predict the occurrence of death within 30 days after admission in the TIC patients(P<0.05), and the predictive efficacy of combining these three indicators was higher［AUC(95%CI)=0.823(0.720-0.925), P<0.001］. Conclusion　Detection of FDP, D-D and PLT indicators at admission is helpful to evaluate the survival prognosis of TIC patients, which is worth the attention of clinical physicians.]]> 2024/5/31 12:04:37 WANG Yaxin, QIAN jing, YANG Wendi, LANG Di, MA Yongxin, ZHANG Hongwei, LI Haisheng, ZHAO Xiaoli 261true ［Abstract］　Objective　To explore the diagnostic value of circulating tumor cells(CTCs) combined with integrin β1(ITG β1) in pathological nipple discharge(PND) patients with intraductal malignant lesions of mammary glands. Methods　The clinical data of 212 patients with PND who were admitted to Beijing Tongzhou Maternal and Child Health Hospital and Beijing Chaoyang Hospital, Capital Medical University from January 2022 to September 2023 were retrospectively analyzed. The research subjects were divided into benign lesion group(187 cases) and malignant lesion group(25 cases) according to the results of pathological examination of mammary ducts. The number of CTCs in the PND patients was detected by using subtraction enrichment and immunostaining-fluorescence in situ hybridization(SE-iFISH), and the serum ITG β1 level was detected by using enzyme-linked immunosorbent assay(ELISA). Multivariate logistic regression was used to analyze the influencing factors of intraductal malignant lesions of mammary glands in the PND patients. Receiver operating characteristic(ROC) curve was used to analyze the diagnostic efficacy of the number of CTCs and serum ITG β1 level on intraductal malignant lesions of mammary glands in the PND patients. Results　There were significant differences in age, body mass index(BMI), menstrual status, type of discharge and breast mass between the two groups(P<0.05). The number of CTCs in the malignant lesion group was larger than that in the benign lesion group, and the serum ITG β1 level in the malignant lesion group was higher than that in the benign lesion group, and the differences were statistically significant(P<0.05). The results of multivariate logistic regression analysis showed that age more than 50 years, BMI more than 24 kg/m², blood discharge, more CTCs and higher serum ITG β1 level were independent risk factors for developing intraductal malignant lesions of mammary glands in the PND patients(P<0.05). Both the number of CTCs and the serum ITG β1 level had the application value in the diagnosis of intraductal malignant lesions of mammary glands in the PND patients(P<0.05), and the combined detection of the two indicators had better diagnostic efficacy［AUC(95%CI)=0.961(0.925-0.983), P<0.001］, and the sensitivity and specificity were 96.00% and 84.49%, respectively. Conclusion　The number of CTCs and serum ITG β1 level are significantly elevated in PND patients with intraductal malignant lesions of mammary glands. The number of CTCs combined with serum ITG β1 level has better diagnostic value for intraductal malignant lesions of mammary glands in the PND patients.]]> 2024/5/31 12:04:37 1, ZHANG Chao², LI Zhi¹, YU Man³, REN Xinling³, TIAN Ziru³, SHI Lihui¹]]> ZHANG Teng¹, ZHANG Chao², LI Zhi¹, YU Man³, REN Xinling³, TIAN Ziru³, SHI Lihui¹ 260true ［Abstract］　Objective　To systematically evaluate the clinical efficacy and safety of platelet-rich plasma(PRP) in treatment of alopecia areata(AA). Methods　China National Knowledge Infrastructure(CNKI), Wanfang medical online, VIP network, Sinomed database, PubMed database, EMBASE database, Cochrane Library database and Web of Science database were searched. During the period from the establishment of the databases to April 2023, the papers were searched and the relevant clinical studies on the treatment of AA with PRP were included, and the evaluation indicators included study indicators involving the Severity of Alopecia Tool(SALT) score or the SALT 6-point score. The efficacy and safety of PRP in treatment of AA were systematically evaluated. Results　A total of 7 studies were included for Meta-analysis. After PRP treatment, the SALT score of the patients decreased significantly(P<0.05). SALT score decreased in both PRP treatment group and steroid treatment group, but there was no statistically significant difference in the SALT score between the two groups［SMD(95%CI)=-0.41(-1.22-0.40), I²=81.00%, P=0.33］. Five studies had mentioned the occurrence of adverse reactions during the treatment of AA with PRP. These adverse reactions were mainly manifested as transient injection site pain, local erythema, burning sensation and edema, which could be recovered within a short time, and AA patients were generally well tolerated for PRP treatment. Conclusion　PRP is safe and effective in treating AA.]]> 2024/5/31 12:04:37 JIANG Yuwei, LIANG Wen, FANG Qiang, LIAO Haiquan, YANG Bin 259true ［Abstract］　Objective　To explore the protective effect of Ramulus Mori(Sangzhi) alkaloids tablets(SZ-A) on obese mice and the impact of SZ-A on gut microbiota. Methods　Male C57BL/6 mice of specific pathogen free(SPF) grade were selected and an obese mouse model was induced in these mice by feeding them with a high-sugar and high-fat diet for 12 weeks. The successfully modeled mice were randomly divided into model group(M group), positive control group(PC group), low-dose SZ-A group(SZ-L group), medium-dose SZ-A group(SZ-M group) and high-dose SZ-A group(SZ-H group), with 10 mice in each group. Additionally, 10 normal mice were set as the blank group(N group). The PC group was given simvastatin suspension(2.6 mg/kg) by gavage. The SZ-L group, the SZ-M group and the SZ-H group were orally administered with SZ-A at doses of 20 mg/kg, 40 mg/kg and 80 mg/kg, respectively. The N group and the M group were given equal volume of distilled water by gavage. All the groups were given intragastric administration once a day for 12 weeks. Lee′s index, body mass, biochemical indicators, pathological change of inguinal white adipose tissues(iWAT), gut microbiota α and β diversity, and relative abundance of gut microbiota were compared among the groups. Results　There were statistically significant differences in Lee′s index, body mass, and serum total cholesterol(TC), triglyceride(TG), high-density lipoprotein cholesterol(HDL-C) and low-density lipoprotein cholesterol(LDL-C) levels among the groups(P<0.05). Adipocytes in the N group were closely arranged, with a large number and small volume of adipocytes in the same field of view. The volume of adipocytes in the M group increased significantly. Compared with that in the M group, the volume of adipocytes in the PC group and the SZ-L group decreased slightly. The number of adipocytes in the SZ-M group and the SZ-H group increased obviously and the cell volume decreased obviously, tending towards those in the N group. The Ace index in the SZ-H group was significantly higher than that in the M group, the PC group and the SZ-L group(P<0.05). The Chao index in the SZ-H group was significantly higher than that in the PC group and the SZ-L group(P<0.05). The Shannon index in the SZ-H group was significantly higher than that in the M group, the PC group, the SZ-L group and the SZ-M group(P<0.05). The Coverage index in each group approached 1. The results of PCoA analysis showed that the separation degrees of the N group, the M group, the PC group, the SZ-L group, the SZ-M group and the SZ-H group were relatively good, and the horizontal linear distance between the N group and the M group was relatively far, and the microbial community structure in the SZ-L group, the SZ-M group and the SZ-H group gradually tended towards that in the N group. The results of non-metric multidimensional scaling(NMDS) analysis showed a Stress value of 0.131. At the phylum level, compared with the M group, the SZ-H group exhibited significantly lower relative abundances of Firmicutes and Patescibacteria(P<0.05), and significantly higher relative abundance of Bacteroidota(P<0.05). At the genus level, compared with the M group, the SZ-H group showed significantly higher relative abundance of norank_f_Muribaculaceae(P<0.05), and significantly lower relative abundances of unclassified_f_Lachnospiraceae, norank_f_norank_o_Clostridia_UCG-014 and Desulfovibrio(P<0.05). Conclusion　SZ-A can partially resist the increase of body mass and the expansion of adipose tissues induced by a high-sugar and high-fat diet in obese mice, improves their lipid metabolism, enhances microbial species diversity, and increases the relative abundances of partial microbial species.]]> 2024/5/31 0:00:00 1, WU Chunwei², CHEN Hailong¹, WANG Ruolin¹, PENG Jin¹, HE Ze²]]> CAO Lei¹, WU Chunwei², CHEN Hailong¹, WANG Ruolin¹, PENG Jin¹, HE Ze² 258true ［Abstract］　Objective　To analyze the clinical, CT imaging and pathological features of 7 cases of lymphocytic interstitial pneumonia(LIP). Methods　The clinical and imaging data of 7 cases of LIP proved pathologically were retrospectively analyzed. All the cases underwent chest thin-section CT examination. Results　All the 7 LIP patients were female, among whom, 4 cases had the manifestation of diffuse patchy ground-glass opacities on their CT images. Among the 4 cases of diffuse patchy ground-glass opacities, 3 cases accompanied with grids and small nodules. Among the 7 LIP patients, 3 cases had the manifestation of focally distributed patchy ground-glass opacities. Among the 3 cases of focally distributed patchy ground-glass opacities, 1 case showed locally mixed small nodules. Among the 7 LIP patients, 5 cases had multiple thin-walled pulmonary cysts containing air, 4 cases accompanied with mediastinal lymph node enlargement. Hematoxylin and eosin(HE) staining of pathological biopsy tissues mainly showed diffuse lymphocyte infiltration in lung interstitium, and the immunohistochemical staining mainly indicated the increase of CD3 cells and polyclonal CD20 cells. Conclusion　LIP is a rare disease, which tends to occur in female patients with Sjgren′s syndrome. When the lungs show patchy ground-glass opacities and changes in multiple thin-walled pulmonary cysts containing air on chest high-resolution CT, and when the pathological manifestation is diffuse lymphocyte infiltration in the lung interstitium, LIP is highly suggested.]]> 2024/5/31 12:04:37 1,2, YANG Xinguan², JIANG Chunrong²]]> LIU Siqiu^1,2, YANG Xinguan², JIANG Chunrong² 257true ［Abstract］　Objective　To explore the expression of miR-4516 in nasopharyngeal carcinoma and its clinicopathological significance. Methods　The clinicopathological data of 60 patients with nasopharyngeal Epstein-Barr virus-related non-keratosis carcinoma(nasopharyngeal carcinoma group) who were admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region from February 2010 to October 2016 were retrospectively analyzed, and 15 patients with chronic nasopharyngeal mucosa inflammation during the same period were selected as control group. Real-time quantitative polymerase chain reaction(RT-PCR) was used to detect the expression level of micro ribonucleic acid-4516(miR-4516) in the mucosal lesion tissues of the two groups and the results were compared between the two groups. The correlation between miR-4516 expression level and the clinicopathological features and survival prognosis of the nasopharyngeal carcinoma patients was analyzed. Receiver operating characteristic(ROC) curve was used to analyze the diagnostic efficacy of miR-4516 in diagnosis of nasopharyngeal carcinoma metastasis. Results　The expression level of miR-4516 in the nasopharyngeal carcinoma group was higher than that in the control group, and the difference was statistically significant(Z=4.477, P<0.001). The expression level of miR-4516 in the mucosal lesion tissues of the nasopharyngeal carcinoma patients with metastasis was higher than that in the patients without metastasis, and the difference was statistically significant(P<0.05). The expression level of miR-4516 was not significantly associated with the patients′ gender, age, tumor site, T stage, and clinical stage(P>0.05). The results of ROC curve analysis showed that miR-4516 could be used in diagnosing the metastasis of nasopharyngeal carcinoma［AUC(95%CI)=0.696(0.541-0.851), P=0.037］, and the optimal cut-off value was 6.28, and the corresponding sensitivity and specificity were 0.63 and 0.75, respectively. The survival prognosis of the low miR-4516 expression group was significantly better than that of the high miR-4516 expression group(log-rank test: χ²=4.230, P=0.040). Conclusion　The expression level of miR-4516 is up-regulated in nasopharyngeal carcinoma tissues, which can be used as a molecular marker to predict metastasis and clinical prognosis evaluation of nasopharyngeal carcinoma.]]> 2024/5/31 12:04:37 1, LI Yanyan¹, ZHANG Lixing¹, LIU Xia¹, ZHANG Shaojie², MO Xianglan¹, YIN Wu¹]]> HUANG Yongta¹, LI Yanyan¹, ZHANG Lixing¹, LIU Xia¹, ZHANG Shaojie², MO Xianglan¹, YIN Wu¹ 256true ［Abstract］　Objective　To analyze the familial variation of SDH gene in three pedigrees with hereditary head and neck paraganglioma. Methods　Three patients with head and neck paraganglioma(probands) who were admitted to Department of Otolaryngology, Head and Neck Surgery, West China Hospital of Sichuan University from January 2022 to December 2023 and their family members were selected. The peripheral venous blood of 200 μL was collected from the study subjects, and their genomic DNA were extracted and sequenced, and the candidate variants were sequenced by using Sanger sequencing and were analyzed for pathogenicity. Results　The three pedigrees were diagnosed with paraganglioma caused by heterozygous variations of SDHD NM_003002.4:c.1A>G, SDHD NM_003002.4:c.274G>T and SDHB NM_003000.3:c.689G>A, respectively, all of which were previously reported variations. Conclusion　Conducting genetic testing on patients with head and neck paraganglioma can clarify the cause of the disease and provide reference for genetic counseling.]]> 2024/4/30 0:00:00 1,2, Mailudan Ainiwaer¹, LU Yu^1,2, CHEN Fei¹]]> GENG Jia^1,2, Mailudan Ainiwaer¹, LU Yu^1,2, CHEN Fei¹ 255true ［Abstract］　Objective　To analyze the correlation between baseline serum hexokinase 1 antibody titer and vision improvement after anti-vascular endothelial growth factor(VEGF) treatment in patients with ischemic macular edema secondary to retinal vein occlusion(RVO-ME). Methods　A total of 53 patients who were diagnosed with ischemic RVO-ME and received initial anti-VEGF treatment in Xuanwu Hospital Capital Medical University from June 2017 to February 2020 were recruited, including 23 cases of ischemic central retinal vein occlusion(CRVO)(CRVO group) and 30 cases of ischemic branch retinal vein occlusion(BRVO group). In addition, 30 cataract patients who underwent phacoemulsification in Xuanwu Hospital Capital Medical University during the same period were selected as the control group. All the subjects underwent baseline serum hexokinase 1 antibody titer detection, routine ophthalmic examination and optical coherence tomography(OCT) examination. All the ROV-ME patients received intravitreal injection of anti-VEGF drugs for treatment according to the “3+ on-demand treatment protocol(pro re nata, PRN)”. After 12 months of follow-up, the influencing factors of vision improvement in patients with ischemic RVO-ME after anti-VEGF treatment was analyzed by using multiple linear regression. Results　The baseline logarithm of the minimum resolution angle unit for best corrected visual acuity(logMAR BCVA) in the CRVO group was higher than that in the control group and the BRVO group, and the baseline central retinal thickness(CRT) and baseline serum hexokinase 1 antibody titer in the CRVO group and the BRVO group were higher than those in the control group, and the baseline CRT and baseline serum hexokinase 1 antibody titer in the CRVO group were higher than those in the BRVO group, and the differences were statistically significant(P<0.05). In the RVO-ME patients, baseline serum hexokinase 1 antibody titer was positively correlated with BCVA improvement 6 months(r=0.377, P=0.005), 9 months(r=0.362, P=0.008) and 12 months(r=0.465, P<0.001) after follow-up, and was positively correlated with the transverse length reduction value of ellipsoid zone(EZ) band interruption 12 months after follow-up(r=0.401, P=0.001). The results of multiple linear regression analysis showed that baseline logMAR BCVA and baseline serum hexokinase 1 antibody titer were influencing factors for BCVA improvement in ischemic RVO-ME patients 12 months after anti-VEGF treatment(P<0.05). Conclusion　Hexokinase 1 antibody is a novel serum biomarker associated with vision improvement after anti-VEGF treatment in patients with ischemic RVO-ME.]]> 2024/4/30 0:00:00 WANG Xizhe, LIU Dachuan, ZHANG Lu, WANG Yenan, LI Zhen, FANG Wei 254true ［Abstract］　Objective　To explore the expressions of fat mass and obesity-associated protein(FTO) and gasdermin D(GSDMD) in gastric cancer(GC) tissues and their mechanisms of action. Methods　The differential expressions of FTO and GSDMD in GC tissues were analyzed by using the Tumor Immune Estimation Resource(TIMER) 2.0 database. Forty-five GC tissues and the corresponding paracancer tissues(3-5 cm away from the tumor margin) which were surgically excised from the patients in the Second Affiliated Hospital of Guangxi Medical University from January 2022 to March 2023 were collected, and the patients′ clinicopathological data were collected. Immunohistochemical staining method was used to detect the expressions of FTO and GSDMD in the tissues, and their correlations with the patients′ clinicopathological features were analyzed. AGS cells with low expression of FTO were constructed by transfecting low expression of FTO lentivirus(FTO low expression group), and the cells transfected with empty vector lentivirus were set up as the control group. Real-time fluorescence quantitative polymerase chain reaction(RT-PCR) was used to detect the levels of FTO mRNA and GSDMD mRNA expressions in the cells in the two groups. Cell scratch wound healing assay and cell migration test were used to analyze the cell migration ability in the two groups. Results　The analysis results based on TIMER 2.0 database and the immunohistochemical staining results showed that FTO and GSDMD were highly expressed in GC tissues(P<0.05). The high expressions of FTO and GSDMD were closely related to the depth of tumor invasion and lymph node metastasis(P<0.05). The results of cell experiment showed that the level of GSDMD mRNA expression in the FTO low expression group was significantly lower than that in the control group(P<0.05), and the scratch healing rate was lower and the number of cell migration was less in the FTO low expression group, and the differences were statistically significant(P<0.05). Conclusion　FTO may promote the metastasis of GC cells by regulating the expression of GSDMD.]]> 2024/4/30 11:24:22 HUANG Shanpei, WEI Erdan, ZHU Liye, NING Qiting, CHEN Xingmei, CHEN Ni, WU Jiangni, QIU Xinze, HUANG Jie′an, LIU Shiquan 253true ［Abstract］　Objective　To explore the effect of transforming growth factor-β(TGF-β) signaling pathway on osteogenic differentiation of odontogenic myxoma(OM) mesenchymal stem cells(OMMSC). Methods　Two patients with OM who were admitted to Stomatological Hospital Affiliated to Medical School of Nanjing University from 2018 to 2020 were selected. The patients′ partial lesion specimens were obtained through surgery for OMMSC isolation and culture. In addition, 5 young patients who received dental implant therapy in the hospital were selected to extract bone marrow from the jaw bone for jaw bone mesenchymal stem cells(JBMSC) isolation and culture. The cells were identified by cell proliferation assay, flow cytometry and alizarin red S staining. After 7 days of osteogenic culture, real-time fluorescence quantitative polymerase chain reaction(RT-PCR) was used to detect the TGF-β signaling pathway and the expression of osteogenesis-related genes. The effects of small molecule drug SB431542 and TGF-β1 interventions on osteogenic differentiation ability of OMMSC and JBMSC were observed. Results　OMMSC and JBMSC obtained by isolation had spindle-shaped morphology, and expressed the surface markers of mesenchymal stem cells, and had osteogenic differentiation ability, which were consistent with the characteristics of mesenchymal stem cells. Compared with JBMSC, OMMSC had stronger proliferation ability. The results of RT-PCR showed that TGF-β/Smad signal-related factors were highly expressed in OMMSC(P<0.05), and the expression of osteogenesis-related factor osteopontin(OPN) was decreased(P<0.05). The results of alizarin red S staining showed that SB431542 could significantly promote the osteogenic differentiation of OMMSC, while TGF-β1 inhibited the osteogenic differentiation of OMMSC. Conclusion　In this study, OMMSC are successfully isolated, and TGF-β signal-related factors are highly expressed in OMMSC, and inhibiting TGF-β signal transduction pathway can enhance the osteogenic ability of OMMSC, which provides reference for improving bone defects in patients with OM.]]> 2024/4/30 11:24:22 1, WANG Zhongshan², SUN Guowen³, CHEN Sheng⁴, TONG Xin³, QIN Haiyan⁵]]> CHEN Xinglin¹, WANG Zhongshan², SUN Guowen³, CHEN Sheng⁴, TONG Xin³, QIN Haiyan⁵ 252true ［Abstract］　Objective　To observe the effect of retinal photocoagulation treatment on ocular accommodation function in patients with myopia. Methods　Forty patients undergoing retinal photocoagulation treatment in the Third People′s Hospital of Huizhou City from July 2022 to July 2023 were recruited. The patients′ distance visual acuity, near visual acuity and ocular accommodation function［including amplitude of accommodation(AMP), binocular positive relative accommodation(PRA), binocular negative relative accommodation(NRA) and binocular cross-cylinder(BCC)］ were examined before retinal photocoagulation treatment and 1 week, 2 weeks, 3 weeks and 1 month after retinal photocoagulation treatment, respectively. Results　There were no significant changes in distance visual acuity, near visual acuity and BCC before and after retinal photocoagulation treatment. AMP and NRA decreased significantly 1 week after treatment(P<0.05), and then gradually increased. However, the patients′ AMP had not returned to the preoperative level 1 month after treatment(P<0.05), and there was no significant difference between the level of NRA 3 weeks after surgery and that before surgery(P>0.05). PRA decreased significantly 1 week after treatment(P<0.05), and then recovered gradually, and there was no significant difference between the level of PRA 1 week after surgery and that before surgery(P>0.05). Conclusion　After 1 week of retinal photocoagulation treatment, the indicators of AMP, NRA and PRA in the patients′ ocular accommodation function decreased, and gradually recovered thereafter. It is recommended that myopic patients receiving retinal photocoagulation treatment choose to undergo myopia correction surgery after 3 weeks of the treatment.]]> 2024/4/30 11:24:22 LI Wentao, WEN Yanmei, ZHONG Li′ai, ZHANG Runmei, YANG Ziying, DUAN Na, LIAO Rui 251true ［Abstract］　Objective　To explore the mechanism of SNHG3 promoting proliferation, migration, invasion and epithelial-mesenchymal transition(EMT) of hepatoma cells by regulating the micro ribonucleic acid(miR)-186-5p/zinc finger E-box binding homeobox 1(ZEB1) axis. Methods　Human-derived hepatoma(HepG2) cells were transfected with small interfering RNA(siRNA) to knock out SNHG3 and transfected with pcDNA3.1(+)/SNHG3 to overexpress SNHG3, and real-time fluorescent quantitative polymerase chain reaction(FQ-PCR) was used to detect whether the transfections were successful. HepG2 cell proliferation was detected by colony formation assay. Cell scratch wound healing assay was used to detect the migration rate of HepG2 cells. Transwell assay was used to detect the number of HepG2 cell invasion. Dual-luciferase reporter assay was used to verify the targeting relationship between SNHG3 and miR-186-5p as well as the targeting relationship between miR-186-5p and ZEB1. The expression levels of SNHG3, ZEB1, E-cadherin, N-cadherin, matrix metalloproteinase-2(MMP-2), vimentin, matrix metalloproteinase-9(MMP-9) and Snail were detected by using FQ-PCR and Western blot. Results　The expression of SNHG3 in hepatocellular carcinoma(HCC) tissues was higher than that in normal tissues, and the survival prognosis of the HCC patients with low/moderate SNHG3 expression was better than that of the HCC patients with high SNHG3 expression, and the differences were statistically significant(P<0.05). The results of cloncolony formation assay showed that compared with that in the pcDNA3.1(+) group, the proliferation of HepG2 cells in the pcDNA3.1(+)/SNHG3 group was significantly increased(P<0.05). The results of cell scratch wound healing assay showed that the migration rate of HepG2 cells in the pcDNA3.1(+)/SNHG3 group was significantly higher than that in the pcDNA3.1(+) group(P<0.05). The results of Transwell assay showed that the number of HepG2 cell invasion in the pcDNA3.1(+)/SNHG3 group was significantly higher than that in the pcDNA3.1(+) group(P<0.05). The results of dual-luciferase reporter assay showed that after co-transfection with miR-186-5p mimic and pGL6-SNHG3-WT, the luciferase activity was lower than the other groups, and after co-transfection with miR-186-5p mimic and pGL6-ZEB1-WT, the luciferase activity was lower than the other groups, and the differences were statistically significant(P<0.05). The results of FQ-PCR and Western blot showed that when SNHG3 was overexpressed, the relative expressions of E-cadherin mRNA and protein were significantly downregulated, while the relative expressions of N-cadherin, MMP-2, vimentin, MMP-9, and Snail mRNA and protein, and the relative expressions of ZEB1 protein were significantly upregulated, and after SNHG3 was knocked out, the relative expressions of E-cadherin mRNA and protein were significantly upregulated, while the relative expressions of N-cadherin, MMP-2, vimentin, MMP-9 and Snail mRNA and protein, and the relative expression of ZEB1 protein were significantly downregulated, and the differences were statistically significant(P<0.05). Conclusion　SNHG3 can promote the proliferation, migration, invasion and EMT of HCC cells by regulating the miR-186-5p/ZEB1, indicating that SNHG3 is a potential therapeutic target for HCC.]]> 2024/4/30 11:24:22 CHEN Xiaolan, SU Yayong, LIU Shuangping, WANG Lihui, XU Chengrun 250true ［Abstract］　Objective　To explore the correlation of obesity with postoperative complications and re-operation in patients with vestibular schwannoma(VS). Methods　The clinical and imaging data of 377 patients with VS who underwent retrosigmoid approach surgery in the First Teaching Hospital of Xinjiang Medical University from June 2013 to June 2023 were retrospectively analyzed. According to body mass index(BMI), the patients were divided into obese group(BMI≥28 kg/m², 91 cases) and non-obese group(BMI<28 kg/m², 286 cases). The clinical data, the incidence rate of postoperative complications and the re-operation rate were compared between the two groups. The influence of BMI on postoperative cerebrospinal fluid leakage and re-operation was explored by using multivariate logistic regression analysis. Results　Compared with the non-obese group, the obese group had higher proportion of male sex, patients complicated with diabetes mellitus and hypertension, and the difference was statistically significant(P<0.05). There were no significant differences in age, hospitalization time, operation side, tumor nature, tumor volume grade and operation time between the two groups(P>0.05). The incidence rate of postoperative cerebrospinal fluid leakage and the re-operation rate in the obese group were higher than those in the non-obese group, and the differences were statistically significant(P<0.05). There were no significant differences in the incidence rates of intracranial hemorrhage, incision infection, intracranial infection, deep vein thrombosis and other postoperative complications between the two groups(P>0.05). After adjusting for age, sex, tumor volume grade and tumor nature, the results of multivariate logistic regression analysis showed that higher BMI level was a risk factor for postoperative cerebrospinal fluid leakage［OR(95%CI)=1.168(1.051-1.299), P=0.004］ and re-operation［OR(95%CI)=1.160(1.053-1.277), P=0.003］. Conclusion　Obesity is closely related to postoperative cerebrospinal fluid leakage and re-operation in VS patients, and the increased BMI is a risk factor for postoperative cerebrospinal fluid leakage and re-operation.]]> 2024/4/30 11:24:22 LI Wenzhuang, WANG Zening, LI Yandong, ZHU Guohua, Maidinaimu Mahemuti, Maimaitili Mijiti, Geng Dangmurenjiafu 249true ［Abstract］　Objective　To analyze the correlation of preoperative left atrial appendage(LAA) opening parameters with the size of the occluders placed intraoperatively in atrial fibrillation(AF) patients undergoing left atrial appendage occlusion(LAAO) and the factors influencing the occurrence of postoperative peri-device leak(PDL). Methods　The clinical data of 83 patients with non-valvular AF who were successfully treated with LAAO in Second Hospital of Shanxi Medical University from January 2019 to December 2022 were retrospectively analyzed.The patients were divided into PDL group(23 cases) and non-PDL group(60 cases) according to the occurrence of postoperative PDL. The clinical data and LAA opening parameters were compared between the patients in the two group. Pearson correlation was used to analyze the correlation of LAA opening parameters with the size of the occluders placed intraoperatively in the non-PDL group. Logistic regression was used to analyze the factors influencing the occurrence of postoperative PDL. Results　The patients in the PDL group were significantly younger than those in the non-PDL group(P<0.05). There were no statistically significant differences in gender and combined underlying diseases between the two groups(P>0.05). The long diameter, short diameter, circumference and area of the LAA opening in the PDL group were greater than those in the non-PDL group, and the differences were statistically significant(P<0.05). The results of Pearson correlation analysis showed that the long diameter, short diameter, circumference and area of the LAA opening in the non-PDL group were positively correlated with the size of the placed occluders(P<0.05). The results of multivariate logistic regression analysis showed that older age was an independent protective factor for the occurrence of postoperative PDL(P<0.05), and larger short diameter of LAA opening was an independent risk factor for the occurrence of postoperative PDL(P<0.05). Conclusion　LAA opening parameters are positively correlated with the size of occluders, and the LAA opening area has the best correlation with the size of the occluders, which provides guidance for the selection of occluders in clinical practice. Older age is an independent protective factor for the occurrence of PDL after LAAO and larger short diameter of LAA opening is an independent risk factor for the occurrence of PDL after LAAO.]]> 2024/4/30 11:24:22 1, WANG Xinwen², YANG Min¹, ZHAO Jimin¹, WANG Jie¹, DING Jinxiang¹, GAO Nanlin¹]]> CHAI Xianing¹, WANG Xinwen², YANG Min¹, ZHAO Jimin¹, WANG Jie¹, DING Jinxiang¹, GAO Nanlin¹ 248true ［Abstract］　Objective　To observe the clinical efficacy of repetitive transcranial magnetic stimulation(rTMS) combined with citalopram hydrobromide in treatment of post-stroke sleep disorder(PSSD). Methods　The clinical data of 94 patients with PSSD who were treated in the First Affiliated Hospital of Xinxiang Medical University from April 2021 to January 2023 were retrospectively analyzed. The patients were divided into control group and combination group according to different treatment plans, with 47 cases in each group. The control group was treated with citalopram hydrobromide and the combination group was treated with rTMS combined with citalopram hydrobromide, and both groups were treated continuously for 8 weeks. The total effective rate, sleep quality at different time points, negative emotion, motor threshold(MT) and the levels of inflammatory factors were compared between the two groups. Results　The total effective rate after treatment in the combination group was significantly higher than that in the control group(95.74% vs 78.72%; χ²=6.114, P=0.013). After treatment, the Pittsburgh Sleep Quality Index(PSQI), Insomnia Severity Index(ISI), Beck Depression Inventory(BDI), Beck Anxiety Inventory(BAI) scores, and the levels of interleukin(IL)-6, tumor necrosis factor-α(TNF-α) and IL-2 showed a downward trend while MT showed an increasing trend, and the differences were statistically significant(P<0.05). After 4 and 8 weeks of treatment, the PSQI, ISI, BDI and BAI scores as well as the levels of IL-6, TNF-α and IL-2 in the combination group were lower than those in the control group, and MT in the combination group was higher than that in the control group, and the differences were statistically significant(P<0.05). Conclusion　The combination of rTMS and citalopram hydrobromide is significantly effective in the treatment of PSSD patients, which can reduce inflammation, increase MT, and improve sleep disorder and negative emotion.]]> 2024/4/30 11:24:22 LI Jingyan, ZHANG Ping 247true ［Abstract］　Objective　To analyze the effects of etomidate and ciprofol on ventricular-arterial coupling(VAC) in elderly hypertension patients during induction of general anesthesia. Methods　A total of 57 elderly hypertension patients who underwent surgical treatment under general anesthesia in Guangzhou Red Cross Hospital from May 2023 to November 2023 were recruited and divided into etomidate group(group E, 28 cases) and ciprofol group(group C, 29 cases) by random number table method. Mean arterial pressure(MAP), heart rate(HR), left ventricle stroke volume(LVSV), effective arterial elastance(E_a), left ventricle end-systolic elastance(E_es) and E_a to E_es ratio(E_a/E_es) were recorded in the two groups before anesthesia induction(T₀), 1 minute after anesthesia induction(T₁), 2 minutes after anesthesia induction(T₂), before tracheal intubation(T₃) and 1 minute after completion of tracheal intubation(T₄). The postoperative extubation time, length of stay at post-anesthesia care unit(PACU), incidence of adverse reactions, use of atropine and phenylephrine and the number of patients with E_a/E_es>1.0 were recorded in the two groups. Results　Compared with those at T₀, E_a/E_es increased at T₁-T₃, and MAP, HR and LVSV decreased at T₁-T₃, and HR increased at T₄, and E_a increased at T₁ and T₂, and E_es decreased at T₂-T₄ in the group E. Compared with those at T₀, E_a/E_es increased at T₁-T₄, and MAP, HR, LVSV and E_es decreased at T₁-T₄, and E_a increased at T₁-T₃ and decreased at T₄ in the group C, and the differences were statistically significant(P<0.05). Compared with the group C, the group E had high HR and LVSV at T₁-T₄, low E_a/E_es at T₁-T₄, high MAP at T₃ and T₄, high E_es at T₁, T₃ and T₄, and low E_a at T₂, and the differences were statistically significant(P<0.05). There were no statistically significant differences in the postoperative extubation time, length of stay at PACU, incidence of adverse reactions, use of atropine and phenylephrine and the proportion of patients with E_a/E_es>1.0 between the two groups(P>0.05). Conclusion　Both etomidate and ciprofol have good safety in the induction of anesthesia in elderly patients with hypertension, but etomidate can better maintain the VAC.]]> 2024/4/30 11:24:22 1, CAO Yang^1,2, PENG Weilong², LUO Jianhua²]]> HUANG Jingxiang¹, CAO Yang^1,2, PENG Weilong², LUO Jianhua² 246true ［Abstract］　Objective　To compare the therapeutic effects between Da Vinci robot surgery by subxiphoid approach and video-assisted thoracoscopic surgery(VATS) by subxiphoid approach for anterior mediastinal tumors. Methods　A total of 56 patients with anterior mediastinal tumors who were admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2020 to January 2023 were recruited and divided into robot-assisted thoracoscopic surgery(RATS) group(n=20) and VATS group(n=36) according to different surgical methods. All the patients were operated using subxiphoid approach. The operation time, intraoperative blood loss, 48-hour drainage volume, catheter retention time, total hospitalization cost, postoperative hospital stay, Visual Analogue Scale(VAS) score and complications were compared between the two groups. Results　In the VATS group, 1 patient underwent a conversion to thoracotomy due to the close adhesion of the innominate vein to the thymus, and 1 patient underwent a conversion to thoracotomy due to the close adhesion of the thymus to the pericardium pleura and the left upper lung. In the RATS group, no patients underwent a conversion to thoracotomy or extended incision length. The operations were successful in the patients in both groups. The operation time of the RATS group was significantly shorter than that of the VATS group(P<0.05), but there were no significant differences in the intraoperative blood loss, 48-hour drainage volume and catheter retention time between the two groups(P>0.05). The postoperative VAS scores in both groups showed a downward trend. The VAS scores of the RATS group on day 1, day 2 and day 3 after surgery were lower than those of the VATS group, and the differences were statistically significant(P<0.05). There was no significant difference in the incidence of complications between the two groups(5.88% vs 0.00%; P=0.525). The hospitalization cost of the RATS group was higher than that of the VATS group, and the difference was statistically significant(P<0.05). There was no significant difference in postoperative hospital stay between the two groups(P>0.05). Conclusion　RATS is a safe and feasible method for the treatment of anterior mediastinal lesions. Compared with VATS, RATS has positive significance in reducing the patients′ postoperative pain and is conducive to their rapid recovery.]]> 2024/4/30 11:24:22 1, ZHOU Yifan², XU Ruihong², SHEN Bin², LUO Jinlong², LI Xiangwei², LIU Songtao², SUN Siyuan¹]]> SUN Zhendong¹, ZHOU Yifan², XU Ruihong², SHEN Bin², LUO Jinlong², LI Xiangwei², LIU Songtao², SUN Siyuan¹ 245true ［Abstract］　Objective　To explore the application value of major histocompatibility complex(MHC)-Ⅱ combined with MHC-Ⅰ immunohistochemical staining in diagnosis of idiopathic inflammatory myopathy(IIM). Methods　A total of 29 specimens were collected from the patients who received inmuscle biopsy in the Department of Neurology of Xuanwu Hospital Capital Medical University during March 2010 and April 2018, and the clinical data of the patients were collected through the hospital electronic medical record system, including 4 types of IIM［dermatomyositis(DM)(5 cases), polymyositis(PM)(5 cases), sporadic inclusion body myositis(IBM)(4 cases) and necrotizing antoimmune myopathy(NAM)(5 cases)］ and 2 types of non-inflammatory myopathy(NIM)［muscular dystrophy(MD)(5 cases), dysferlinopathy myopathy(5 cases)］. Hematoxylin-eosin(HE) staining and MHC-Ⅰ and MHC-Ⅱ immunohistochemical staining were performed on the specimens. Results　The results of immunohistochemical staining showed that the positive rate of MHC-Ⅰ in the muscle specimens of PM, DM and IBM patients was 100.0%, and the positive rate of MHC-Ⅰ in the muscle specimens of NAM and MD patients was 80.0%, and the positive rate of MHC-Ⅰ in the muscle specimens of dysferlinopathy myopathy patients was 20.0%. The positive rate of MHC-Ⅱ in the muscle specimens of PM and IBM patients was 40.0% and 50.0%, respectively. The immunohistochemical staining of MHC-Ⅱ in the muscle specimens of the patients with other types of diseases was negative. Conclusion　MHC-Ⅱ staining has higher specificity than MHC-Ⅰ staining in distinguishing IIM from NIM, and MHC-Ⅱ combined with MHC-Ⅰ immunohistochemical staining has better clinical application value in the diagnosis of different types of myopathy.]]> 2024/4/3 11:46:24 DUO Jianying, DI Li, LU Yan, WANG Min, HUANG Yue, ZHU Wenjia, WEN Xinmei, XU Min, CHEN Hai, DA Yuwei 244true ［Abstract］　Objective　To analyze the clinicopathological features and prognostic influencing factors of SMARCA4-deficient non-small-cell lung cancer(NSCLC) patients. Methods　The clinical data of 106 patients with SMARCA4-deficient NSCLC(SMARCA4-deficient group) and 212 patients with non-SMARCA4-deficient NSCLC(non-SMARCA4-deficient group) who were admitted to Dongnan Hospital of Xiamen University and Zhangzhou Municipal Hospital of Fujian Province from January 2016 to June 2021 were retrospectively analyzed. Survival curve was plotted by Kaplan-Meier method, and comparison between groups was performed by log-rank test. Cox regression was used to analyze the factors influencing the 2-year overall survival of the SMARCA4-deficient NSCLC patients. Results　Compared with those in the non-SMARCA4-deficient group, the patients in the SMARCA4-deficient group had older age, higher proportion of male and smoking history, longer tumor diameter, higher Ki-67 index, lower proportion of EGFR mutation, lower proportion of squamous cell carcinoma, and higher proportion of poorly differentiated carcinoma, and the differences were statistically significant(P<0.05). Among patients with SMARCA4-deficient NSCLC, 95.28%(101/106) of the patients had complete deficiency of BRG1 protein, and 8.20%(5/61) of the patients were CK7-negative, and 85.11%(80/94) of the patients were CK5/6 -negative, and 77.36%(82/106) of the patients were TTF-1 -negative, and 80.19%(85/106) of the patients were P40-negative, and 66.67%(16/24) of the patients were CD34 -negative, and 56.52%(13/23) of the patients were SALL4-negative.The 2-year survival of the patients with non-SMARCA4-deficient NSCLC was better than that of the patients with SMARCA4-deficient NSCLC(log-rank test: χ²=126.837, P<0.001). Male gender, increase of age, having smoking history, and longer tumor diameter were independent risk factors for shortening the survival time of the SMARCA4-deficient NSCLC patients. Conclusion　SMARCA4-deficient NSCLC is a type of tumor that differs from non-SMARCA4-deficient NSCLC in that it has a poorer prognosis and a higher tumor aggressiveness.]]> 2024/4/3 11:46:24 1, ZHANG Ping², WANG Minjie¹, LAN Jiaqi³, LIN Bingqi¹]]> TANG Yu¹, ZHANG Ping², WANG Minjie¹, LAN Jiaqi³, LIN Bingqi¹ 243true ［Abstract］　Objective　To analyze the clinical features and prognosis of the acute exacerbation of chronic obstructive pulmonary disease(AECOPD) patients with different inflammatory phenotypes. Methods　The clinical data of 255 patients with AECOPD who were admitted to the Department of Respiratory Medicine of Northern Jiangsu People′s Hospital from January 2021 to April 2022 were retrospectively analyzed. The patients were divided into eosinophil(EOS) group(87 cases), neutrophil group(92 cases), mixed granulocyte group(30 cases) and agranulocytosis group(46 cases) according to their initial peripheral blood cell count results at admission. The clinical features and the readmission rates of the patients admitted to hospital for AECOPD within one year after discharge were compared among the four groups. Results　There were statistically significant differences in white blood cell count(WBC), EOS percentage(EOS%), EOS, neutrophil(NE) percentage(NE%), NE, lymphocyte(LY) percentage(LY%), LY, neutrophil count to lymphocyte count ratio(NLR), C-reactive protein(CRP), procalcitonin(PCT) and albumin(ALB) levels among the patients in the four groups(P<0.05). The neutrophil group had higher WBC, NE%, NE, NLR, CRP and PCT levels, and lower EOS%, EOS, LY%, LY and ALB levels compared with the EOS group. The mixed granulocyte group had higher WBC, NE%, NE and NLR levels, and lower LY%, LY and ALB levels than the EOS group, and the mixed granulocyte group had higher EOS% and EOS levels than the neutrophil group. Compared with the neutrophil group and the mixed granulocyte group, the agranulocytosis group had lower WBC, NE%, NE, NLR and PCT levels, and higher LY% and LY levels. EOS% and EOS levels in the agranulocytosis group were lower than those in the EOS group and the mixed granulocyte group, while CRP level in the agranulocytosis group was lower than that in the neutrophil group. The differences were statistically significant(P<0.05). There were statistically significant differences in the pulmonary function indexes among the patients in the four groups(P<0.05). The neutrophil group had lower forced expiratory volume in one second as a percentage of the predicted value(FEV₁%pred) and forced expiratory volume in one second(FEV₁)/forced vital capacity(FVC) levels than the EOS group. FEV₁%pred and FEV₁/FVC levels in the agranulocytosis group were higher than those in the neutrophil group, while FEV₁/FVC level in the agranulocytosis group was higher than that in the mixed granulocyte group. The differences were statistically significant(P<0.05). There were statistically significant differences in the hospitalized days, utilization rates of intravenous corticosteroids and antibiotics among the patients in the four groups(P<0.05). The neutrophil group had more hospitalized days and higher utilization rate of intravenous corticosteroids than the EOS group, and the neutrophil group had higher utilization rate of antibiotics than the EOS group and the agranulocytosis group. The differences were statistically significant(P<0.05). Kaplan-Meier survival curves showed that the readmission rates of the patients admitted to hospital for AECOPD were ranked from high to low in the neutrophil group(61.06%), the mixed granulocyte group(54.78%), the agranulocytosis group(45.23%) and the EOS group(42.89%), and the log-rank test showed statistically significant differences among the 4 groups(χ²=10.816, P=0.013). Conclusion　There are significant differences in inflammatory indicators, nutritional status, pulmonary function, hospitalized days, therapeutic effect and prognosis among the AECOPD patients with four different inflammatory phenotypes based on peripheral blood cell count. The AECOPD patients with neutrophilic type show more severe clinical features and have a higher rate of readmission to hospital for AECOPD within one year after discharge than those with eosinophilic type.]]> 2024/4/3 11:46:24 ZHU Yajing, LIU Juan, QIN Yan, WANG Lijun 242true ［Abstract］　Objective　To explore the effects of illumination intensity on eye biological parameters, retinal structure, and expression levels of lumican and matrix metalloproteinase-2(MMP-2) in scleral tissues of optical defocus myopia guinea pigs. Methods　A total of 80 healthy 3-week-old guinea pigs were selected, regardless of gender. The models of guinea pigs with right-eye optical defocused low to moderate myopia were constructed by concave lens induction method. Among them, 60 guinea pigs were randomly selected from 74 successfully modeled guinea pigs and divided into group A, group B, group C and group D, with 15 guinea pigs in each group. The group A was exposed to outdoor sufficient sunlight for 3 hours every day and the average illumination was 60 000 lux. The group B was placed in a cool outdoor place for 3 hours every day and the average illumination was 10 000 lux. The group C were placed at the indoor north window for 3 hours every day and the average illumination was 2 000 lux. The group D was raised indoors normally every day with an average illumination of 300 lux. In the rest of the time, all the guinea pigs were given the same indoor feeding with an average illumination of 300 lux, normal food and water intake, and continuous illumination treatment for 6 months. The ocular biological parameters(axial length and diopter) of the guinea pigs were compared among different groups, and the retinal pathology and ultrastructure of the guinea pigs were observed, and the expression levels of lumican and MMP-2 in the scleral tissues were detected. Results　Compared with those before light exposure, the diopter increased in different degrees in each group after 1 month, 3 months and 6 months of light exposure, and the group A showed varying degrees of increase in axial length at 3 and 6 months of light exposure, while the group B, the group C and the group D showed varying degrees of increase in axial length at 1, 3, and 6 months of light exposure, and the differences were significant(P<0.05). After 1 month of light exposure, the ocular diopter and axial length in the group A and the group B were lower than those in the group D, and after 3 and 6 months of light exposure, the ocular diopter and axial length in the group A were lower than those in the group B, the group C and the group D, and the ocular diopter and axial length in the group B were lower than those in the group D, and the differences were significant(P<0.05). The number of apoptotic cells in the group A and the group B was less than that in the group C and the group D, and the number of apoptotic cells in the group A was less than that in the group B, and the differences were significant(P<0.05). The damage degree of retinal structure in the group A and the group B was milder than that in the group C and the group D, and the damage degree in the group A was milder than that in the group B. After 6 months of light exposure, compared with those in the group C and the group D, the expression levels of lumican were higher and the expression levels of MMP-2 were lower in the group A and the group B, and the expression levels of lumican in the group A were higher than those in the group B, and the expression levels of MMP-2 in the group A were lower than those in the group B, and the differences were significant(P<0.05). Conclusion　Outdoor high-intensity light exposure can up-regulate lumican expression and down-regulate MMP-2 expression in the scleral tissues of guinea pigs, and can stabilize scleral structure, which can slow down the growth of ocular axial length and myopia progression.]]> 2024/4/3 11:46:24 FU Junda, ZHU Haiping, YANG Wanchen, JIA Na, SUN Yijun 241true ［Abstract］　Objective　To analyze the effect of median nerve repetitive peripheral magnetic stimulation(rPMS) combined with conventional rehabilitation training on upper limbs and hand function in pediatric patients with spastic hemiplegia. Methods　A total of 61 pediatric patients with spastic hemiplegia who were admitted to the Department of Rehabilitation of Xuzhou Children′s Hospital from June 2022 to June 2023 were recruited. The pediatric patients were divided into observation group(30 cases) and control group(31 cases) by random number table method. The pediatric patients in both groups received conventional rehabilitation training. The observation group received median nerve rPMS before the conventional rehabilitation training, and the control group received median nerve magnetic pseudo-stimulation before the conventional rehabilitation training. Caroll Upper Extremity Functional Test(UEFT), Box and Block Test(BBT) and Fudan Infant Hand Function Assessment(Chinese version)—Mini-Manual Ability Classification System(Mini-MACS) were used to evaluate the effects of upper limbs and hand function in pediatric patients with spastic hemiplegia before treatment and 4 weeks after treatment, respectively. Results　Compared with those before treatment, the grasping ability and coordination of the pediatric patients in the two groups were significantly improved after treatment, and their UEFT total scores and BBT scores were significantly elevated, and the differences were statistically significant(P<0.05). After treatment, the improvement of grasping ability in the observation group was better than that in the control group, and the UEFT total scores and BBT scores in the observation group were higher than those in the control group, and the differences were statistically significant(P<0.05). There was no significant difference between pre-therapeutic Mini-MACS grading and post-therapeutic Mini-MACS grading in the two groups(P>0.05). After treatment, the Mini-MACS grading in the observation group was significantly better than that in the control group(P<0.05). Conclusion　Median nerve rPMS combined with conventional rehabilitation training can effectively improve upper limbs and hand function in pediatric patients with spastic hemiplegia.]]> 2024/4/3 11:46:24 WANG Min, QIU Aizhen, WU Ye, MENG Lulu 240true ［Abstract］　Objective　To explore the correlation of changes in serum miR-133a levels with thyroid hormone and lipid metabolism in hyperthyroidism patients. Methods　A total of 100 patients with new onset of dominant hyperthyroidism who were admitted to the 983rd Hospital of the Chinese People′s Liberation Army Joint Logistics Support Force from October 2020 to August 2022 were recruited as the hyperthyroidism group, of whom 28 patients received standard methimazole treatment. Other 100 healthy individuals who underwent physical examination during the same period were selected as the control group. The clinical data such as gender, age, thyroid hormone, liver function and biochemical indicators of the research subjects were collected. The serum miR-133a level was detected by using real-time fluorescence quantitative polymerase chain reaction(PCR), and the correlation of serum miR-133a level with thyroid hormone and lipid metabolism levels was analyzed. Results　Compared with those in the control group, the levels of free thyroxine(FT₄), free triiodothyronine(FT₃), thyroid-stimulating hormone receptor antibody(TRAb), diastolic blood pressure, alanine aminotransferase(ALT), total bilirubin(TBIL) and direct bilirubin(DBIL) in the hyperthyroidism group were increased, while the levels of body mass index(BMI), waist circumference, total cholesterol(TC), triglyceride(TG), low-density lipoprotein cholesterol(LDL-C) and thyroid-stimulating hormone(TSH) in the hyperthyroidism group were decreased, and the differences were statistically significant(P<0.05). The serum miR-133a level in the hyperthyroidism group was significantly lower than that in the control group(Z=9.796, P<0.001). In the hyperthyroidism group, the serum miR-133a level was negatively correlated with ALT, FT₃ and FT₄ levels(P<0.05), and was positively correlated with TC, LDL-C and TSH levels(P<0.05). After receiving treatment with methimazole, the hyperthyroidism patients had significantly decreased FT₃ and FT₄ levels(P<0.05), and significantly increased TC, LDL-C, TSH and serum miR-133a levels(P<0.05). The changes in serum miR-133a levels before and after treatment were negatively correlated with the changes in FT₃(r_s=-0.728, P<0.001) and FT₄ levels(r_s=-0.377, P=0.048), but were not significantly correlated with the changes in TC, TG, LDL-C and TSH levels(P>0.05). Conclusion　The serum miR-133a levels in hyperthyroidism patients are down-regulated, which may be related to the lipid metabolism disorders caused by elevated thyroid hormone.]]> 2024/4/3 11:46:24 HUANG Baole, WU Feng, YUE Yue, ZHENG Guiling 239true ［Abstract］　Objective　To explore the relationship between serum uric acid(sUA) levels and patch characteristics of culprit vessels in patients with acute coronary syndrome(ACS). Methods　The clinical data of 268 patients with ACS who were admitted to the Department of Cardiovascular Medicine of Yantai Yuhuangding Hospital, Qingdao University from January 2018 to October 2022 were retrospectively analyzed, and the patients were divided into high sUA group(≥6 mg/dL, 117 cases) and low sUA group(<6 mg/dL, 151 cases) according to sUA levels. The clinical data as well as coronary artery angiography and optical coherence tomography(OCT) examination data were compared between the two groups. The relationship between sUA levels and patch characteristics of culprit vessels was analyzed by using multivariate regression. Results　The body mass index level of the high sUA group was higher than that of the low sUA group, and the proportion of males and patients complicated with hyperlipidemia in the high sUA group was higher than that in the low sUA group, and the patients in the high sUA group were younger than those in the low sUA group, and the differences were statistically significant(P<0.05). The levels of hemoglobin, creatinine, triglyceride, lipoprotein a and homocysteine in the high sUA group were higher than those in the low sUA group, and the differences were statistically significant(P<0.05). The rate of collateral circulation formation in the low sUA group was higher than that in the high sUA group, and the difference was statistically significant(P<0.05). However, there were no statistically significant differences in the other coronary artery angiography indexes between the two groups(P>0.05). The rate of luminal stenosis, proximal lumen reference area, distal lumen reference area, red thrombosis rate and macrophage infiltration rate in the high sUA group were higher than those in the low sUA group, and the differences were statistically significant(P<0.05). The results of multivariate regression analysis showed that the increase of sUA level was a risk factor for promoting red thrombosis［OR(95%CI)=1.490(1.071-2.075),P=0.018］ and the occurrence of macrophage infiltration［OR(95%CI)=1.278(1.045-1.563), P=0.017］, and was positively correlated with proximal lumen reference area［β(95%CI)=0.492(0.155-0.828), P=0.004］. Conclusion　The increase of sUA level is a risk factor for promoting red thrombosis and the occurrence of macrophage infiltration, which deserves the attention of clinicians.]]> 2024/4/3 11:46:24 1, LIU Weifeng², WANG Hua², REN Mengmeng², YU Liangliang², ZHONG Lin²]]> SU Xiutian¹, LIU Weifeng², WANG Hua², REN Mengmeng², YU Liangliang², ZHONG Lin² 238true ［Abstract］　Objective　To observe the effects of blood flow restriction(BFR) training with different contraction modes on the function and morphology of vastus medialis oblique(VMO) in patients with knee osteoarthritis(KOA). Methods　A total of 108 patients with KOA who were admitted to Kunming Municipal Hospital of Traditional Chinese Medicine from June 2021 to June 2023 were recruited and divided into observation group A, observation group B and control group by using random number table method, with 36 cases in each group. The control group received isotonic contraction training of VMO, and the observation group A received isometric contraction training of VMO combined with BFR training, and the observation group B received isotonic contraction training of VMO combined with BFR training. Visual Analogue Scale(VAS) scores, thigh circumference, VMO thickness, and VMO electromyographic values［including root-mean-square(RMS) value and integrated electromyography(iEMG)］ were compared among the three groups before and after treatment. Results　After 3 weeks of treatment, VAS scores of the left knee and the right knee in the three groups were significantly decreased compared with those before treatment(P<0.05), and the circumferences of the left thigh and the right thigh, VMO thickness, iEMG value and RMS value were significantly increased compared with those before treatment(P<0.05), and the improvements of the indicators in the observation group B and the observation group A were better than those in the control group(P<0.05), and the indicators in the observation group B were more significantly improved(P<0.05). Conclusion　BFR training can effectively improve joint pain, increase VMO thickness and thigh circumference in KOA patients. Under the condition of low load BFR, the rehabilitation treatment effect of isotonic contraction training is better than that of isometric contraction training.]]> 2024/4/3 11:46:24 1, FENG Yu², CHEN Xiaoxia¹, CHEN Yuefeng³, LI Weiren¹, HUANG Li¹, YAO Na¹]]> LI Pengcheng¹, FENG Yu², CHEN Xiaoxia¹, CHEN Yuefeng³, LI Weiren¹, HUANG Li¹, YAO Na¹ 237true ［Abstract］　Objective　To explore the effect of gestational diabetes mellitus(GDM) on neonatal left ventricular function. Methods　Ninety-eight full-term neonates delivered by gestational diabetes mellitus(GDM) mothers who were admitted to the Department of Neonatology of Qinzhou Maternal and Child Health Care Hospital from January 2022 to July 2022 were selected as the GDM group. According to the blood glucose control levels of the mothers during pregnancy, the patients in the GDM group were further divided into the blood glucose control meeting standards group(63 cases) and the blood glucose control failing to meet standards group(35 cases). Three hundred and eighty-three full-term neonates delivered by mothers without GDM who were admitted to the same hospital during the same period were selected as the control group. The serum myocardial enzyme profile, septal thickness and systolic peak strain level of each ventricular septal segment(basal ventricular septum, medial ventricular septum and apical ventricular septum) were compared between the neonates in the two groups. Results　There were no significant differences in neonatal lactate dehydrogenase(LDH), creatine kinase(CK), creatine kinase lsoenzyme-MB(CK-MB) and cardiac troponin I(cTnI) between the GDM group and the control group(P>0.05). Compared with those in the control group, the thickness of the neonatal ventricular septum increased, and the septal strain of basal, middle and apical segments were lower in the GDM group, and the differences were statistically significant(P<0.05). Compared with those in the blood glucose control meeting standards group, the thickness of the neonatal ventricular septum increased, and the septal strain of basal, middle and apical segments were lower than those in the blood glucose control failing to meet standards group, and the differences were statistically significant(P<0.05). Conclusion　The ventricular septa of the neonates delivered by the mothers with GDM are thicker than those of the normal full-term neonates, and these neonates delivered by the GDM mothers have myocardial mechanical changes in their left ventricles, and the changes are more obvious in the neonates delivered by the GDM mothers with blood glucose control failing to meet standards. Clinicians should pay more attention to the perinatal health care of the mothers with GDM to help them control blood glucose level.]]> 2024/4/3 11:46:24 1, LIANG Meixin², HUANG Peilu¹, BI Lei¹, HUANG Guosheng¹, HUANG Haiyan¹]]> LU Anfeng¹, LIANG Meixin², HUANG Peilu¹, BI Lei¹, HUANG Guosheng¹, HUANG Haiyan¹ 236true ［Abstract］　Objective　To explore the clinical application value of optimization of magnetic resonance gradient echo T^*₂ weighted imaging(GRE-T2^*₂WI) sequence parameters in patients with acute ischemic stroke(AIS). Methods　A total of 50 AIS patients were selected from the People′s Hospital of Wuzhou during June 2017 and December 2018, including 38 males and 12 females, aged 41 to 92(65.50±5.90)years. GRE-T2^*₂WI examination was performed on each patient using four parameters, and four groups of images were obtained. Group A was the control group, and group B had the reduced matrix, number of excitation(NEX) and time of repetition(TR) on the basis of group A, and group C adopted parallel acquisition technique and had the reduced NEX, TR and echo time(TE) on the basis of group B, and group D had the further reduced TR, TE and matrix on the basis of group C. The scanning time, image quality score, signal-to-noise ratio(SNR) and contrast-to-noise ratio(CNR) of the images were compared among the four groups. Results　The scanning time in group A, group B, group C and group D was 81 seconds, 40 seconds, 15 seconds and 11 seconds, respectively. There were significant differences in the image quality scores, SNR and CNR of the parameters among the four groups(P<0.05). The image quality scores in group A, group B, group C and group D were (3.56±0.28)points, (3.34±0.34)points, (3.00±0.32)points and (1.68±0.40)points, respectively. There were significant differences in the image quality scores between group C and group D(P<0.05). There were no significant differences in the image quality scores between group C and group A as well as between group C and group B(P>0.05). Group D had the highest level of SNR, and there were significant differences in the levels of SNR between group C and group A as well as between group C and group B(P<0.05). There was no significant difference in the level of SNR between group C and group D(P>0.05). Group C had the highest level of CNR, and there were no significant differences in the levels of CNR between group C and group A as well as between group C and group D(P>0.05). There was significant difference in the level of CNR between group C and group B(P<0.05). Conclusion　After adopting parallel acquisition technique and appropriately reducing matrix NEX, TR and TE parameters, the scanning time of GRE-T^*₂WI is significantly shortened, and its image quality meets the diagnostic requirements, which has great application value in the diagnosis and treatment of AIS patients.]]> 2024/4/3 0:00:00 1, YANG Weizhen¹, CHEN Peng^1,2, LI Ruixiong¹, YAN Yejing¹, WANG Tianzhao¹]]> LI Yanhua¹, YANG Weizhen¹, CHEN Peng^1,2, LI Ruixiong¹, YAN Yejing¹, WANG Tianzhao¹ 235true ［Abstract］　Objective　To explore the effects of different surgical approaches on levels of postoperative circulating tumor cells(CTCs) in patients with early papillary thyroid carcinoma(PTC). Methods　The clinical data of 109 patients with early PTC who were admitted to the Second Nanning People′s Hospital from December 2017 to March 2022 were retrospectively analyzed. Among them, 78 patients underwent traditional open surgery(group A), and 14 patients(group B) underwent endoscopic thyroidectomy via axillary approach, and 17 patients(group C) underwent endoscopic thyroidectomy via submental vestibular approach. The differences in perioperative indicators, postoperative hospital stay, and postoperative CTCs types and levels were compared among the three groups. Results　The intraoperative bleeding volume and the number of lymph nodes dissected in group A were greater than those in group B and group C, and the postoperative extubation time and the duration of postoperative hospital stay in group A were longer than those in group C, and the differences were statistically significant(P<0.05). The postoperative extubation time in group B was significantly longer than that in group C(P<0.05). There was no significant difference in the operation time among the three groups(P>0.05). There was no significant difference in the positive rate of CTCs among the three groups(P>0.05). There were significant differences in total CTCs count and mixed CTCs count among the three groups(P<0.05), among which group C had the highest levels of postoperative counts. Conclusion　The three surgical approaches have good efficacy and safety for early PTC. Different approaches have a certain effect on the level of postoperative CTCs, but the effects of these differences on the prognosis of the patients still need to be further verified.]]> 2024/4/3 0:00:00 WANG Huiling, PAN Xiaoming, LYU Mian, HUANG Yonghong, XU Changqiang 234true ［Abstract］　Objective　To explore the application effect of robot-assisted high-selection puncture technique in percutaneous vertebroplasty. Methods　The clinical data of 70 patients with osteoporotic vertebral compression fracture who were admitted to Beijing Haidian Hospital from July 2020 to January 2022 were retrospectively analyzed. All the patients underwent percutaneous vertebroplasty(PVP) and were divided into observation group(robot-assisted, 35 cases) and control group(C-arm fluoroscopy-assisted, 35 cases) according to different adjuvant treatment methods. The operation-related indicators, preoperative and postoperative pain Visual Analogue Scale(VAS) scores, lumbar Oswestry Disability Index(ODI) scores, Japanese Orthopaedic Association(JOA) scores, vertebral Cobb angle, length of hospital stay, and postoperative complications were compared between the two groups. Results　The intraoperative fluoroscopy times and invisible blood loss in the observation group were less than those in the control group, and the operation time in the observation group was shorter than that in the control group, and the differences were statistically significant(P<0.05). Compared with those in the two groups before operation, the VAS scores and ODI scores were low, and the JOA scores were high, and the Cobb angle of vertebral body was small after operation, and the differences were statistically significant(P<0.05). After operation, the VAS scores and ODI scores in the observation group were lower than those in the control group, and the JOA scores in the observation group were higher than those in the control group, and the Cobb angle of vertebral body in the observation group was smaller than that in the control group, and the differences were statistically significant(P<0.05). The incidence rate of complications in the observation group was lower than that in the control group, and the length of hospital stay in the observation group was shorter than that in the control group, and the differences were statistically significant(P<0.05). Conclusion　For patients with osteoporotic vertebral compression fracture, robot-assisted high-selection puncture technique can be chosen for performing PVP on them and helps the operation go smoothly, improves the lumbar function of the patients, reduces the impact of low back pain on the patients′ normal life, and the safety of the operation is relatively high. This method is worthy of promotion.]]> 2024/3/15 0:00:00 SHI Yan, WEI Zhiling, MENG Lingxin, SONG Huawei 233true ［Abstract］　Objective　To analyze the clinicopathological features of 9 cases of lung adenocarcinoma with signet-ring cell-like morphology. Methods　The clinical data of 9 cases of lung adenocarcinoma with signet-ring cell-like morphology who were admitted to Affiliated Hospital of North Sichuan Medical College and Nanchong Central Hospital from January 2013 to December 2019 were retrospectively analyzed, and their pathology, imageology and clinical features, treatment regimens and survival prognosis were analyzed. Results　Among the 9 patients, there were 3 males and 6 females, aged 34-74 years, with a median age of 53 years. The patients′ TNM stages included stage Ⅰ in 1 case, stage Ⅱ in 1 case, stage Ⅲ in 5 cases and stage Ⅳ in 2 cases. The proportion of signet-ring cell components ranged from 10% to 100%, and 4 cases suffered from lung adenocarcinoma with simple signet-ring cell components. All the 9 cases showed the immunophenotypes of transcription termination factor-1(TTF-1)(+) and cytokeratin 7(CK7)(+)/cytokeratin 20(CK20)(-). Five patients underwent surgical resection, and 3 patients received non-surgical treatment, and 1 patient lost the opportunity for surgery due to the tumor invasion into the main bronchus, pulmonary artery, superior vena cava and pericardium. The overall survival time was 6-61 months. Conclusion　Lung adenocarcinoma with signet-ring cell-like morphology is a rare type of adenocarcinoma that can exhibit the immunophenotypes of TTF-1(+) and CK7(+)/CK20(-) and the diagnosis of this disease mainly relies on pathological examination. At present, there is not a standard treatment regimen for this disease, and the survival prognosis of the patients is poor, and targeted therapy may have the opportunity to improve the patients′ prognosis.]]> 2024/3/15 19:12:41 1,2, HE Fang^1,2, GONG Haiying³, JIAN Shunhai⁴, WU Cailiang⁵, JIN Xiuli⁶, JIANG Li^1,2]]> HE Liping^1,2, HE Fang^1,2, GONG Haiying³, JIAN Shunhai⁴, WU Cailiang⁵, JIN Xiuli⁶, JIANG Li^1,2 232true ［Abstract］　Objective　To analyze the early predictive value of triglyceride-glucose(TyG) index, glucose-to-lymphocyte ratio(GLR) combined with bedside index for severity in acute pancreatitis(BISAP) score on the severity of hypertriglyceridemic acute pancreatitis(HTG-AP). Methods　The clinical data of 166 patients with HTG-AP who were admitted to the Wuhan Third Hospital from May 2018 to May 2023 were retrospectively analyzed, including 113 cases in the non-severe group and 53 cases in the severe group. The clinical data collected within 24 hours of admission were compared between the two groups. Multivariate logistic regression was used to analyze the independent influencing factors of severe HTG-AP. Receiver operating characteristic(ROC) curve was used to analyze the efficacy of TyG index, GLR and BISAP score in predicting severe HTG-AP. Results　The proportion of the patients with hypertension and diabetes in the severe group was higher than that in the non-severe group, and the hospital stay in the severe group was longer than that in the non-severe group, and the levels of lymphocytes(LYM) and calcium(Ca²⁺) in the severe group were lower than those in the non-severe group, and the levels of red blood cell volume distribution width(RDW), D-dimer(DDI), C-reactive protein(CRP), fasting plasma glucose(FPG), total cholesterol(TC), triglyceride(TG), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C), TyG index, GLR level, and the BISAP scores in the severe group were higher than those in the non-severe group, and the differences were statistically significant(P<0.05). The results of multivariate logistic regression analysis showed that higher levels of TyG index, GLR and BISAP score were the independent risk factors for promoting the occurrence of severe HTG-AP(P<0.05). The results of ROC curve analysis showed that TyG index, GLR and BISAP score all had application value in predicting the occurrence of severe HTG-AP(P<0.05), and BISAP score combined with TyG index or GLR had better prediction efficacy than a single index. Conclusion　TyG index, GLR and BISAP score were correlated with the severity of HTG-AP. TyG index or GLR combined with BISAP score had higher early prediction value for the severity of HTG-AP.]]> 2024/3/15 19:12:41 1, TIAN Xia², BAO Sachula¹, WU Junyi¹, HAN Zheng², LIU Meng²]]> LI Shuangping¹, TIAN Xia², BAO Sachula¹, WU Junyi¹, HAN Zheng², LIU Meng² 231true ［Abstract］　Objective　To observe the application effect of remimazolam on elderly patients undergoing heart valve replacement surgery under cardiopulmonary bypass(CPB). Methods　Three hundred and thirty-four elderly patients admitted to People′s Hospital of Zhengzhou for elective cardiac valve replacement surgery under CPB from June 2022 to June 2023 were recruited and divided into remimazolam group(group R) and propofol group(group P) by random number table method, with 167 cases in each group, and according to the exclusion criteria, 160 and 159 cases were finally included in the group R and the group P, respectively. The group R was injected with remimazolam intravenously at 0.2-0.3 mg/kg during anesthesia induction, and was pumped with remimazolam intravenously at 0.5-1.0 mg/(kg·h) during anesthesia maintenance. The group P was injected with propofol intravenously at 1.0-2.0 mg/kg during anesthesia induction, and was pumped with propofol intravenously at 4-10 mg/(kg·h) during anesthesia maintenance. The rest of the anesthesia induction and maintenance were consistent in both groups. The occurrence of postoperative delirium(POD) was assessed by using confusion assessment method-intensive care unit(CAM-ICU) within 3 days after surgery. The indicators related to surgery and anesthesia, the occurrence of POD, Visual Analogue Scale(VAS) score, Ramsay Sedation Scale score, and intraoperative and postoperative adverse events were compared between the two groups. Results　Compared with those in the group P, the patients in the group R had shorter postoperative anesthesia recovery time, shorter tracheal catheter removal time, shorter intensive care unit(ICU) stay time and hospital stay time, and the differences were statistically significant(P<0.05). There were no significant differences in the incidence rate, severity and duration of POD between the two groups(P>0.05). There were no significant differences in the postoperative VAS scores and Ramsay Sedation Scale scores between the two groups(P>0.05) at postoperative different time points. The incidence rates of intraoperative sinus bradycardia and hypotension in the group R were lower than those in the group P, and the differences were statistically significant(P<0.05). Conclusion　Compared with that of propofol, the application of remimazolam in the induction and maintenance of general anesthesia in elderly patients undergoing heart valve replacement surgery under CPB does not increase the incidence rate of POD, and it is safe and helpful for rapid postoperative recovery of the patients.]]> 2024/3/15 19:12:41 FAN Qing, ZHU Shuangshuang, MENG Huifang, ZHANG Xungong 230true ［Abstract］　Objective　To construct Wharton′s jelly(WJ)-modified polycaprolactone(PCL) electrospinning artificial blood vessel scaffolds and to analyze their performances. Methods　The WJ tissues were decellularized by using trypsin method, and histological staining and biochemical component quantification analysis were employed to analyze the efficiency of WJ tissue decellularization and the differences in the biochemical components of the WJ tissues before and after decellularization. Enzyme-linked immunosorbent assay(ELISA) was used to detect the differences in the levels of bioactive factors of the WJ tissues before and after decellularization. The processed WJ was mixed with PCL to prepare spinning solution, and the electrospinning technique was utilized to fabricate tubular WJ/PCL electrospinning artificial blood vessel scaffolds, and their nano-fiber structures, fiber diameter distributions, biomechanical properties and blood compatibilities were analyzed. Results　Trypsin-based decellularization effectively removed cellular nuclear components from WJ matrix, preserving most of the biochemical components of the WJ matrix［including glycosaminoglycan(GAG), proteins, fibers, hyaluronic acid, and hydroxyproline］ and the bioactive factors of the WJ matrix［including vascular endothelial growth factor(VEGF), platelet-derived growth factor(PDGF), transforming growth factor-beta 1(TGF-β1), and fibroblast growth factor-2(FGF-2)］. The electrospinning WJ/PCL artificial blood vessel scaffolds prepared by the electrospinning technique exhibited a typical nano-fiber structure, and their characteristic peaks of both WJ and PCL were verified by Fourier-transform infrared spectroscopy(FTIR). WJ/PCL electrospinning artificial blood vessel scaffolds demonstrated good biomechanical properties and blood compatibilities, effectively inhibiting platelet adhesion. Conclusion　WJ is an ideal natural material that can be used in combination with synthetic materials like PCL to prepare electrospinning artificial blood vessel scaffolds with desirable biological properties.]]> 2024/3/15 0:00:00 HE Fangling, JIANG Tingbo 229true ［Abstract］　Objective　To analyze the application value of FIRST simplified frailty scale score in patients with newly diagnosed multiple myeloma(NDMM). Methods　The clinical data of 131 patients with NDMM who were admitted to the Third Hospital of Shanxi Medical University from July 1, 2014 to December 31, 2021 were retrospectively analyzed. The patients were divided into frailty group(50 cases) and non-frailty group(81 cases) according to different FIRST simplified frailty scale scores. The therapeutic effect, cumulative incidence of early grade ≥3 infection and survival prognosis were compared between the patients in the two groups. Subgroup analysis was performed for the patients receiving bortezomib-based induction therapy scheme. Results　There was no significant difference in the proportion of the patients with therapeutic effect being more than or equal to very good partial response(VGPR) between the two groups(45.68% vs 36.00%, P>0.05). There were 26 cases(52.00%) and 27 cases(33.33%) of early grade ≥3 infection in the frailty group and the non-frailty group, respectively. The prognosis of the patients related to early grade ≥3 infection in the frailty group was worse than that in the non-frailty group(log-rank test: χ²=4.472, P=0.020). The median overall survival(OS) time and median progression-free survival(PFS) time in the frailty group was shorter than that in the non-frailty group(36 months vs 81 months; 16 months vs 34 months), and the survival prognosis of the non-frailty group was significantly better than that of the frailty group(P<0.05). Among the 100 patients receiving the therapy of bortezomib-based induction scheme, both the median OS time and the median PFS time in the frailty group was shorter than that in the non-frailty group(34 months vs 84 months; 15 months vs 33 months), and the survival prognosis of the non-frailty group was significantly better than that of the frailty group(P<0.05). Conclusion　FIRST simplified frailty scale score has relatively good application value for patients with NDMM.]]> 2024/3/15 19:12:41 1,2, WANG Xinwei¹, YANG Hui², LI Linyu², ZHAO Jie², HE Shaolong², TIAN Weiwei²]]> YAO Qiong^1,2, WANG Xinwei¹, YANG Hui², LI Linyu², ZHAO Jie², HE Shaolong², TIAN Weiwei² 228true ［Abstract］　Objective　To explore the current status of literature researches on rat models of primary hepatocellular carcinoma, and to summarize and evaluate these literature researches. Methods　The phrases of “primary hepatocellular carcinoma” and “rat model” were used as key words to retrieve the literature in the China National Knowledge Infrastructure(CNKI) and Wanfang databases. The retrieved papers were indexed by the two databases from January 1996 to December 2021 and 115 pieces of valid literature were included finally. The modeling methods, types and dosages of modeling drugs, modeling cycle and evaluation of the modeling results of the included literature were summarized and analyzed. Results　The most commonly used type of primary hepatocellular carcinoma modeling rat was Wistar rat. The main modeling inducers included 7 inducers such as the aqueous solution prepared with diethylnitrosamine(DEN), DEN+sterilized drinking water, DEN+carbon tetrachloride(CCL₄), DEN+normal saline, DEN+standard feed, 2-acetylaminofluorene(2-AAF) and aflatoxin B₁(AFB₁), among which the aqueous solution prepared with DEN was the most commonly used, accounting for 63.5%. There were 6 types of modeling methods, including free drinking method, intraperitoneal injection method, intraperitoneal injection+free drinking method, gastric gavage method, subcutaneous injection+free drinking method and intraperitoneal injection+gastric gavage, among which free drinking method was the most commonly used method, accounting for 35.7%. The modeling cycle was 10 weeks to 24 weeks, and the modeling results were judged mainly through objective evaluation method, accounting for 47.8%. Conclusion　There are various methods for modeling primary hepatocellular carcinoma rats, and there are not unified standard methods at present. Researchers should choose the appropriate modeling method according to their own experimental needs.]]> 2024/3/15 19:12:41 XU Liang, WANG Yaqi, QIAO Jianliang 227true ［Abstract］　Objective　To observe the therapeutic effect of nephron sparing surgery on giant renal hamartoma. Methods　The clinical data of 35 patients with giant renal hamartoma who were admitted to the Urinary Surgery Center of the First Teaching Hospital of Xinjiang Medical University for nephron sparing surgery from January 2015 to June 2022 were collected. In all the cases, sufficient renal parenchyma was retained while the tumors were removed by transabdominal open surgery or laparoscopic resection, and the surgical effects of the patients were summarized and analyzed. Results　All the operations were completed successfully in the 35 cases. In the patients undergoing laparoscopic resection, there were no cases of conversion to open surgery and no ruptured bleeding. The operation time was 65-265(139.82±54.94)minutes and the blood loss was 20-1 100(279.72±310.42)millilitres.The cold ischemia time was 15-65(36.43±13.92)minutes in 22 cases of open surgery. The warm ischemia time was 20-40(26.47±4.96)minutes in 13 cases of laparoscopic surgery. The serum creatinine value was 36-110(69.99±17.54)μmol/L 3 to 5 days after surgery. The postoperative extubation time was 3-11(4.84±1.63)days, and the postoperative hospitalization time was 4-12(6.71±1.73)days. The renal emission computed tomography(ECT) score of bilateral renal function examination was performed in 18 cases after surgery. The glomerular filtration rate(GFR) of the affected kidneys was 28.7-57.6(37.38±8.15)mL·min^-1·(1.73 m²)^-1, and during the follow-up of 3-87(46.16±26.97)months, their renal function was normal and no tumor recurrence was found. Conclusion　Nephron sparing surgery is safe and feasible for treatment of giant renal hamartoma in technologically mature medical centers.]]> 2024/3/15 19:12:41 HOU Yakun, ZHOU Xingyu, Baihetiya·Azati, Mulati·Rexiati, WANG Yujie, WANG Wenguang 226true ［Abstract］　Objective　To compare the early postoperative visual quality between femtosecond laser small incision lenticule extraction(SMILE) and wavefront aberration guided laser in situ keratomileusis(WG-FS-LASIK) in patients with low myopia without astigmatism. Methods　Fifteen patients(30 eyes, SMILE group) undergoing SMILE treatment and 15 patients(30 eyes, WG-FS-LASIK group) undergoing WG-FS-LASIK treatment in the Optometry Center of the People′s Hospital of Guangxi Zhuang Autonomous Region from March 2022 to June 2022 were selected. All the patients had no astigmatism before surgery and their best corrected visual acuities requiring for the minimum spherical lens power were less than or equal to -3.00 D. Visual acuity, total aberrations(coma, trefoil and spherical aberration), objective scatter index(OSI), modulation transfer function cut-off frequency(MTF_cut-off), Streeler ratio(SR), and predictable visual acuity(PVA)(including PVA 100%, PVA 20% and PVA 9%) were measured before surgery, and 1 day, 1 week, and 1 month after surgery. The measurement results of the observation indicators were compared between the two groups. Results　The equivalent spherical lens and visual acuities were significantly improved in the two groups after surgery compared with those before surgery(P<0.05), but there was no significant difference at each time point between the two groups(P>0.05). The OSI, SR and PVA 9% were improved in both groups after surgery compared with those before surgery(P<0.05). The OSI levels in the WG-FS-LASIK group were significantly decreased 1 day, 1 week and 1 month after surgery compared with those in the SMILE group(P<0.05). One month after surgery, PVA 9% in the WG-FS-LASIK group was significantly higher than that in the SMILE group(P<0.05). The postoperative coma differences in the SMILE group and the WG-FS-LASIK group showed a trend of increasing first and then decreasing, but the changing range of the WG-FS-LASIK group was smaller. The postoperative coma differences in the WG-FS-LASIK group were less than those in the SMILE group 1 day, 1 week and 1 month after surgery, and the differences were statistically significant(P<0.05). Conclusion　Both SMILE and WG-FS-LASIK can improve early postoperative visual quality in patients with low myopia without astigmatism, but the patients undergoing WG-FS-LASIK treatment have better early visual recovery and better nighttime visual quality improvement.]]> 2024/3/15 19:12:41 KONG Min, LUO Wuqiang, LI Lili, XIAO Xin, CHEN Qi, LIN Enwei, LIN Hong, GAN Yu 225true ［Abstract］　Objective　To explore the effect of micro ribonucleic acid-3189-3p(miR-3189-3p) on prognosis of patients with colorectal cancer(CRC) and its mechanism of action. Methods　Based on the data of CancerMIRNome database and ENCORI database, the differences in the expressions of miR-3189-3p in CRC tissues and normal tissues were analyzed, and the effect of the level of miR-3189-3p expression on the survival prognosis of the patients was explored. Hsa-miR-3189-3p mimics and miR-3189-3p NC were transfected into LOVO cells, respectively. The effects of miR-3189-3p on the expression levels of the key proteins(vimentin and N-cadherin) of epithelial-mesenchymal transition(EMT) in the LOVO cells were explored by using Western blot assay. The effects of miR-3189-3p on the migration and invasion ability of the LOVO cells were explored by using scratch assay and Transwell assay. Results　The level of miR-3189-3p expression in the CRC tissues was significantly higher than that in the normal tissues(P<0.05). The survival prognosis of the CRC patients with high expression of miR-3189-3p was better than that of the CRC patients with low expression of miR-3189-3p(P<0.05). After overexpression of miR-3189-3p, the migration and invasion ability of the LOVO cells were significantly weakened(P<0.05), and the expression levels of vimentin and N-cadherin proteins were significantly decreased(P<0.05). Conclusion　MiR-3189-3p may affect the survival prognosis of the patients by regulating the migration, invasion and EMT of CRC cells.]]> 2024/3/15 19:12:41 1, LIANG Haoyuan¹, LI Lintao¹, JIANG Kaihai¹, HUANG Tianfu¹, LI Guangsheng¹, HUANG Xusen²]]> CAO Yusheng¹, LIANG Haoyuan¹, LI Lintao¹, JIANG Kaihai¹, HUANG Tianfu¹, LI Guangsheng¹, HUANG Xusen² 224true ［Abstract］　Objective　To explore the value of global registry of acute coronary events(GRACE) score combined with white blood cell(WBC) count to mean platelet volume ratio(WMR) in predicting the occurrence of in-hospital major adverse cardiovascular events(MACE) after emergency percutaneous coronary intervention(PCI) in acute ST-segment elevation myocardial infarction(STEMI) patients. Methods　The clinical data of 349 STEMI patients who received emergency PCI treatment in Xiangyang Central Hospital from January 2021 to March 2022 were retrospectively analyzed. According to the occurrence of in-hospital MACE, the patients were divided into MACE group(n=47) and non-MACE group(n=302). The clinical data of the patients in different groups were compared. Cox regression was used to analyze the risk factors for the occurrence of in-hospital MACE after PCI in the STEMI patients, and ROC curve analysis was used to evaluate the value of GRACE score combined with WMR in predicting the occurrence of in-hospital MACE after PCI in the STEMI patients. Results　Compared with the non-MACE group, the MACE group were older, and had lower systolic blood pressure, diastolic blood pressure and left ventricular ejection fraction(LVEF), and higher heart rate, rates of Killip grade≥Ⅱ, GRACE score, WBC, WMR, neutrophil-to-lymphocyte ratio(NLR), fasting plasma glucose(FPG), creatinine(Cr), alanine aminotransferase(ALT), aspartate transaminase(AST), and creatine kinase MB isoenzyme(CK-MB), and the differences were statistically significant(P<0.05). The results of multivariate Cox regression analysis showed that higher levels of GRACE score and WMR were independent risk factors for promoting the occurrence of in-hospital MACE in the STEMI patients(P<0.05). The results of ROC curve analysis showed that both GRACE score and WMR were effective in predicting the occurrence of in-hospital MACE after PCI in the STEMI patients(P<0.05), and the combined prediction efficiency of the two indicators was better［AUC(95%CI)=0.824(0.750-0.897), P<0.001］, and the sensitivity and specificity were 74.50% and 80.50%, respectively. Conclusion　The combination of GRACE score and WMR has good predictive value for in-hospital MACE in STEMI patients after PCI, which helps clinical workers to make more accurate risk stratification and treatment decisions.]]> 2024/2/29 11:51:54 1,2, LI Bin², LU Ming², REN Yong², ZHOU Qing², WU Xuan²]]> ZHANG Wanyao^1,2, LI Bin², LU Ming², REN Yong², ZHOU Qing², WU Xuan² 223true ［Abstract］　Objective　To explore the expression of engulfment and cell mobility 3(ELMO3) in pancreatic cancer tissues and its clinical significance. Methods　One hundred and twenty-eight pancreatic carcinoma specimens were collected from the General Hospital of Xinjiang Military Region(63 cases) and Luoyang Central Hospital Affiliated to Zhengzhou University(65 cases) between January 2000 and December 2007, and other 11 cases of normal pancreatic tissue specimens adjacent to pancreatic carcinoma(about 3 cm) were collected as controls. Western blot method and immunohistochemical staining method were used to detect the expressions of ELMO3 in pancreatic carcinoma tissues and adjacent normal pancreatic tissues, and the correlation between ELMO3 expression and the clinicopathological indicators and the prognosis of the pancreatic carcinoma patients were analyzed. Results　The positive rate of ELMO3 in the pancreatic carcinoma tissues was higher than that in the adjacent normal pancreatic tissues, and the difference was statistically significant(83.59% vs 27.27%; χ²=16.199, P<0.001). The results of Western blot detection showed that the ELMO3 expression level in the pancreatic carcinoma tissues was significantly higher than that in the adjacent normal pancreatic tissues(t=1.205, P=0.001). The expression of ELMO3 was significantly associated with tumor diameter, differentiation degree, TNM stage and lymph node metastasis in the pancreatic carcinoma patients(P<0.05). The median survival time of the ELMO3-positive group was shorter than that of the ELMO3-negative group(11 months vs 35 months), and the survival prognosis of the ELMO3-negative group was better than that of the ELMO3-positive group(log-rank test: χ²=58.602, P<0.001). The results of Cox regression analysis showed that ELMO3-positive expression, distant metastasis and TNM stage Ⅲ/Ⅳ were independent risk factors for poor survival prognosis of the pancreatic carcinoma patients(P<0.05). Conclusion　ELMO3-positive expression is associated with malignant clinicopathological characteristics of pancreatic carcinoma and poor prognosis.]]> 2024/2/29 11:51:54 1, GUO Cairu², LIU Mengmeng³, LIU Jiangwei⁴, REN Guoyin⁵]]> JIA Fuxin¹, GUO Cairu², LIU Mengmeng³, LIU Jiangwei⁴, REN Guoyin⁵ 222true ［Abstract］　Objective　To analyze coronary computed tomography angiography(CCTA) high-risk plaque characteristics and correlation of the degree of coronary stenosis with abnormal coronary flow reserve fraction in patients with obstructive coronary artery disease(CAD). Methods　The clinical data of 139 patients who underwent CCTA and were diagnosed with CAD in the First Affiliated Hospital of Hebei North University from January 2017 to June 2018 were retrospectively analyzed, and 205 coronary arteries were enrolled. All the enrolled coronary arteries were divided into abnormal computed tomography(CT)-derived fractional flow reserve(FFR_CI) group(FFR_CI≤0.80, 117 vessels) and normal FFR_CI group(FFR_CI>0.80, 88 vessels) according to different values of FFR_CI. The characteristics of high-risk plaques and the degree of coronary stenosis were compared between the two groups. The effects of the degree of coronary stenosis and the characteristics of high-risk plaques on FFR_CI abnormalities were analyzed by using logistic regression. Results　The incidence rates of positive remodeling(PR) and low attenuation plaque(LAP) in the abnormal FFR_CI group were significantly higher than those in the normal FFR_CI group(P<0.05). There was a statistically significant difference in the degree of coronary artery stenosis between the two groups(P<0.05). Severe coronary stenosis, LAP and PR were independent risk factors of promoting abnormal FFR_CI(P<0.05). Conclusion　CCTA severe coronary artery stenosis, LAP and PR are closely related to abnormal FFR_CI, which is helpful to guide clinical practice to better identify patients with hemodynamic abnormalities.]]> 2024/2/29 11:51:54 1, CHEN Rong², YANG Zhixiang², YANG Yue², PANG Zhiying¹, JIA Peng³, CUI Shujun¹, WANG Dawei⁴]]> YANG Fei¹, CHEN Rong², YANG Zhixiang², YANG Yue², PANG Zhiying¹, JIA Peng³, CUI Shujun¹, WANG Dawei⁴ 221true ［Abstract］　Objective　To explore the value of serum eotaxin(Eot) and human β-defensin-1(hBD-1) in the differential diagnosis of active pulmonary tuberculosis(ATB), and to analyze the relationship between them and clinical efficacy. Methods　A total of 162 ATB patients admitted to Panzhihua Central Hospital from March 2020 to October 2022 were selected(ATB group), and 81 healthy individuals undergoing physical examination(healthy group) and 81 patients with latent tuberculosis infection(LTBI group) were selected during the same period. Serum Eot and hBD-1 levels were detected and compared among the three groups. Receiver operating characteristic(ROC) curve was used to analyze their differential diagnostic value for LTBI and ATB. All the patients in the ATB group were treated with 2HRZE/4HR regimen, and these patients were divided into effective group and ineffective group according to their clinical efficacy, and the serum Eot and hBD-1 levels were compared between the two groups. The risk factors affecting the efficacy of the ATB patients were analyzed using multivariate logistic regression analysis. The predictive value of serum Eot and hBD-1 for efficacy were analyzed using ROC curve. Results　The levels of serum Eot in the ATB group and the LTBI group were higher than those in the healthy group, and the levels of serum hBD-1 in the ATB group and the LTBI group were lower than those in the healthy group, and the differences were significant(P<0.05). The levels of serum Eot in the ATB group were higher than those in the LTBI group, and the levels of serum hBD-1 in the ATB group were lower than those in the LTBI group, and the differences were significant(P<0.05). The results of ROC curve analysis showed that the levels of serum Eot and hBD-1 had the differential diagnostic value for LTBI and ATB(P<0.05), and the combination of the two indicators had better differential diagnostic efficacy［AUC(95%CI)=0.834(0.696-0.969), P<0.001］, with sensitivity and specificity being 83.33% and 82.72%, respectively. In the 162 patients with ATB, the effective rate was 76.54%(124/162) and the ineffective rate was 23.46%(38/162). Smoking history, tuberculosis complicated with tuberculous cavity and the levels of Eot in the ineffective group were significantly higher than those in the effective group(P<0.05), and the levels of hBD-1 in the ineffective group were significantly lower than those in the effective group(P<0.05). The results of multivariate logistic regression analysis showed that smoking history, tuberculosis complicated with tuberculous cavity, higher level of Eot and lower level of hBD-1 were independent risk factors for ineffective treatment of ATB(P<0.05). The results of ROC curve analysis showed that both serum Eot and hBD-1 levels could effectively predict the efficacy of the ATB patients(P<0.05), and the combined predictive efficacy of the two indicators was better［AUC(95%CI)=0.842(0.720-0.940), P<0.001］, with sensitivity and specificity being 81.58% and 82.26%, respectively. Conclusion　Increased serum Eot levels and decreased hBD-1 levels in ATB patients are helpful to distinguish LTBI from ATB, and higher Eot levels and lower hBD-1 levels are independent risk factors for ineffective treatment in the ATB patients. The combined detection of serum Eot and hBD-1 has a higher reference value in predicting the efficacy of the ATB patients.]]> 2024/2/29 11:51:54 1, FENG Jing¹, GONG Chengli¹, DAI Shiqiong², LENG Wenhua³, YANG Wenjuan¹]]> QIU Guoyuan¹, FENG Jing¹, GONG Chengli¹, DAI Shiqiong², LENG Wenhua³, YANG Wenjuan¹ 220true ［Abstract］　Objective　To analyze the correlation of serum neutrophil-to-lymphocyte ratio(NLR), hypersensitive C-reactive protein(hs-CRP) and chitinase-3-like protein 1(CHI3L1) levels with stroke-associated pneumonia in elderly patients with acute cerebral infarction(ACI). Methods　Four hundred and eighty-seven elderly patients with ACI who were admitted to Taixing People′s Hospital from August 2020 to March 2022 were recruited and divided into SAP group(n=139) and non-SAP group(n=348) according to the occurrence of SAP. The clinical data were compared between the two groups, and multivariate logistic regression analysis was used to explore the factors affecting the occurrence of SAP in the ACI patients. The efficiency of serum NLR, hs-CRP and CHI3L1 levels in predicting the development of SAP in the ACI patients was evaluated using receiver operating characteristic(ROC) curve. Results　The results of multivariate logistic regression analysis showed that higher levels of baseline of National Institutes of Health Stroke Scale(NIHSS) scores, NLR, hs-CRP and CHI3L1 and occurrence of dysphagia were independent risk factors for SAP in the ACI patients(P<0.05), while higher baseline of diastolic blood pressure(DBP) was a protective factor against SAP in the ACI patients(P<0.05). The results of ROC curve analysis showed that serum NLR, hs-CRP and CHI3L1 levels could all effectively predict the occurrence of SAP in the ACI patients(P<0.05), and the combination of the three indicators could further improve the prediction efficiency［AUC(95%CI)=0.825(0.783-0.868), P<0.001］, and the sensitivity and specificity were 77.04% and 78.28%, respectively. Conclusion　The elevated levels of serum NLR, hs-CRP and CHI3L1 are all independent influencing factors for the occurrence of SAP in elderly patients with ACI, and the combined detection of the three indicators has higher predictive value.]]> 2024/2/29 11:51:54 YU Baiyang, XU Wu 219true ［Abstract］　Objective　To explore the effect of circular RNA forkhead box protein O3(CircFoxo3) regulating micro ribonucleic acid-130a-5p(miR-130a-5p)/TEA domain transcription factor 1(TEAD1) axis on myocardial cell apoptosis in heart failure(HF) rats. Methods　Sprague-Dawley(SD) rats were divided into control group, HF group, si-NC group, si-CircFoxo3 group, agomir NC group, miR-130a-5p agomir group, si-CircFoxo3+antagomir NC group and si-CircFoxo3+miR-130a-5p antagomir group, with 18 rats in each group. Except for the rats in the control group, the rats in the other groups were injected with doxorubicin hydrochloride intraperitoneally to construct HF rat models. After successful modeling, medication was administered once every 3 days for 3 weeks. Quantitative reverse transcription polymerase chain reaction(qRT-PCR) was applied to detect the expression levels of CircFoxo3 and miR-130a-5p in the myocardial tissues. Color Doppler ultrasound was applied to detect the levels of left ventricular end systolic diameter(LVESD), left ventricular end diastolic diameter(LVEDD), and left ventricular ejection fraction(LVEF) in the rats. Enzyme-linked immunosorbent assay(ELISA) method was applied to detect the levels of human stromelysin-2(ST_r) and N-terminal pro-brain natriuretic peptide(NT-pro BNP) in the serum of the rats. Haematoxylin-eosin(HE) staining and Masson staining were used to detect the pathological changes and the myocardial fibrosis in the myocardial tissues of the rats. TUNEL staining was applied to detect the level of cardiomyocyte apoptosis. Western blot method was applied to detect the expression levels of TEAD1, B cell lymphoma-2(Bcl-2), Bcl-2-associated X protein(Bax), and cleaved aspartate-specific cysteine protease-3(cleaved caspase-3) in the myocardial tissues of the rats. Double luciferase reporter gene experiment was applied to verify the relationship between CircFoxo3 and miR-130a-5p, and between miR-130a-5p and TEAD1. Results　Compared with those in the control group, the rats in the HF group had pathological injury of myocardial tissues and severe myocardial fibrosis, elevated levels of LVESD and LVEDD, elevated levels of CircFoxo3, TEAD1, Bax and cleaved caspase-3 protein expressions in the myocardial tissues, elevated levels of ST_r and NT-pro BNP in the serum, elevated cardiomyocyte apoptosis rate, and decreased levels of LVEF, miR-130a-5p and Bcl-2 protein, and the differences were significant(P<0.05). Compared with those in the HF group and the si-NC group, the rats in the si-CircFoxo3 group had mitigated pathological injury of myocardial tissues, decreased levels of LVESD and LVEDD, decreased expression levels of CircFoxo3, TEAD1, Bax and cleaved caspase-3 protein expression in the myocardial tissues, decreased levels of ST_r and NT-pro BNP in the serum, decreased cardiomyocyte apoptosis rate, and elevated levels of LVEF, miR-130a-5p and Bcl-2 protein, and the differences were significant(P<0.05). Compared with those in the HF group and the agomir NC group, the rats in the miR-130a-5p agomir group had improved pathological injury and myocardial fibrosis in the myocardial tissues,decreased levels of LVESD and LVEDD, decreased levels of TEAD1, Bax, cleaved caspase-3 protein expressions in the myocardial tissues, decreased levels of ST_r and NT-pro BNP in the serum, decreased cardiomyocyte apoptosis rate, and elevated levels of LVEF, miR-130a-5p and Bcl-2 protein, and the differences were significant(P<0.05). Compared with those in the si-CircFoxo3 group and the si-CircFoxo3+antagomir NC group, the rats in the si-CircFoxo3+miR-130a-5p antagomir group had aggravated pathological injury and myocardial fibrosis in the myocardial tissues, elevated levels of LVESD and LVEDD, elevated levels of TEAD1, Bax, cleaved caspase-3 protein expressions in the myocardial tissues, elevated levels of ST_r and NT-pro BNP in the serum, elevated cardiomyocyte apoptosis rate, and decreased levels of LVEF, miR-130a-5p and Bcl-2 protein, and the differences were significant(P<0.05). CircFoxo3 had a targeted relationship with miR-130a-5p, and miR-130a-5p had a targeted relationship with TEAD1. Conclusion　Silencing CircFoxo3 may inhibit the expression of TEAD1 via sponging miR-130a-5p upwards, thereby inhibiting cardiomyocyte apoptosis in HF rats.]]> 2024/2/29 11:51:54 1, LE Jinhai², CHEN Xinyu¹, LIU Jianhe¹, LIU Yuemei¹, LI Jun³, LI Su¹]]> CAO Yanhua¹, LE Jinhai², CHEN Xinyu¹, LIU Jianhe¹, LIU Yuemei¹, LI Jun³, LI Su¹ 218true ［Abstract］　Objective　To observe the anticoagulant application effect of nafamostat mesilate(NM) on continuous blood purification(CBP) in patients with high bleeding risk. Methods　The clinical data of 34 critically ill patients with high bleeding risk who needed CBP treatment in the Department of Critical Care Medicine of the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2023 to March 2023 were retrospectively collected. The patients were divided into NM group(10 cases), sodium citrate group(10 cases) and low molecular weight heparin group(14 cases) according to the use of anticoagulants. The filter service life and the occurrence of clinically significant bleeding events and other adverse events were compared among the three groups. Results　The filter service life was 40.00(17.00, 51.00)hours, 36.00(22.00,60.00) hours and 51.00(45.00,61.50)hours in the NM group, the sodium citrate group and the low molecular weight heparin group, respectively, and there was no significant difference among the three groups(H=2.096, P=0.351). During the anticoagulation process, there were no clinically significant bleeding events in the NM group, while there were 3 cases(30.00%) and 4 cases(28.57%) of clinically significant bleeding events in the sodium citrate group and the low molecular weight heparin group, respectively. The difference in the incidence of clinically significant bleeding events among the three groups was statistically significant(χ²=14.171, P=0.001). Six cases of secondary thrombocytopenia occurred in the low molecular weight heparin group. The adverse reactions related to NM infusion, such as allergic reactions, hyperkalemia and agranulocytosis did not occur in the NM group. There were no adverse reactions such as accumulation of citric acid and citric acidosis in the sodium citrate group. Thromboembolic events were not observed in the patients in the three groups. Conclusion　The anticoagulant effect of NM is comparable to that of sodium citrate and low molecular weight heparin, and the incidence of clinically significant bleeding events of NM is lower, so the safety of application of NM in the patients with high bleeding risk is good.]]> 2024/2/29 0:00:00 1, XIANG Shulin^1,2, REN Yanwen¹, HU Kunlin¹, PANG Jing¹, MO Li¹, XIONG Bin^1,2]]> LU Wenting¹, XIANG Shulin^1,2, REN Yanwen¹, HU Kunlin¹, PANG Jing¹, MO Li¹, XIONG Bin^1,2 217true ［Abstract］　Objective　To analyze the correlation of serum amyloid A(SAA) with pulmonary diffusion function and exercise limitation in elderly patients with chronic obstructive pulmonary disease(COPD). Methods　A total of 188 elderly patients with stable COPD who were admitted to Chongqing University Fuling Hospital from January 2020 to July 2021 were recruited. All the patients underwent pulmonary function test(PFT) and cardiopulmonary exercise testing(CPET) evaluations. The patients with transfer factor for carbon monoxide(T_LCO) of lungLCO≥LLN were included in the normal diffusion function group(86 cases). SAA levels were determined using enzyme-linked immunosorbent assay(ELISA). Pearson correlation analysis was used to investigate the correlation between SAA level and peak oxygen uptake(VO_2peak). Multivariate logistic regression was used to analyze the influencing factors of pulmonary diffusion function in the elderly patients with COPD. Multivariate linear regression model was used to analyze the correlation of SAA level with Z-score of forced expiratory volume in the first second(FEV_1Z-score) and FEV₁ as a percentage of predicted value(ppFEV₁) in the elderly COPD patients. Receiver operating characteristic(ROC) curve was used to analyze the predictive efficacy of SAA level on pulmonary diffusion dysfunction in the elderly patients with COPD. Results　The results of multivariate logistic regression analysis showed that lower ppFEV₁, Z-score of forced vital capacity(FVC_Z-score), (FEV₁/FVC)_Z-score were independent risk factors for promoting pulmonary diffusion dysfunction in the elderly COPD patients(P<0.05), and higher FVC as a percentage of predicted value(ppFVC), FEV₁/FVC, and Z-score of alveolar ventilation(VA_Z-score) were independent protecting factors for inhibiting pulmonary diffusion dysfunction in the elderly COPD patients(P<0.05). The SAA level in the abnormal diffusion function group was higher than that in the normal diffusion function group［(135.09±58.05)ng/mL vs (78.10±32.81)ng/mL, t=8.079, P<0.001］. The results of ROC curve analysis showed that SAA level had application value in predicting pulmonary diffusion dysfunction in the elderly COPD patients［AUC(95%CI)=0.797(0.735-0.860), P<0.001］, and the optimal cut-off value was 85.07 ng/mL. The corresponding sensitivity and specificity were 77.50% and 69.80%, respectively. The results of multivariate linear regression analysis showed that for each unit decrease in FEV_1Z-score and ppFEV₁, the SAA level of the patients in the abnormal diffusion function group was increased by 0.009(95%CI: -0.013--0.005)ng/mL and 0.149(95%CI: -0.210--0.088)ng/mL. The results of Pearson correlation analysis showed that SAA level was negatively correlated with VO_2peak in the elderly patients with COPD(r=-0.512, P<0.001). Conclusion　In elderly COPD patients, SAA level is associated with FEV_1Z-score, and detecting the SAA level can help to identify the elderly COPD patients with pulmonary diffusion dysfunction. In addition, there is a negative correlation between SAA level and VO_2peak, suggesting that the mechanism leading to FEV₁ reduction may help to explain the exercise limitation in the elderly COPD patients with pulmonary diffusion dysfunction.]]> 2024/2/29 11:51:55 OU Yang, ZHONG Canye, ZHANG Shutang, WANG Wei 216true ［Abstract］　Objective　To explore the effect of the changes in microstructure of arcuate fasciculus on mild cognitive function in patients with arcuate fasciculus lacunar cerebral infarction in the walking area based on magnetic resonance diffusion tensor imaging(DTI) technique. Methods　A total of 64 patients with arcuate fasciculus lacunar cerebral infarction in the walking area who were admitted to People′s Hospital of Shuangyashan City between September 2022 and June 2023 were selected. According to the Montreal Cognitive Assessment(MoCA) scale scores, the patients were divided into the mild cognitive impairment(MCI) group(35 cases with MoCA scale scores ≥16 and <26) and the non-MCI group(29 cases with MoCA scale scores ≥26). During the same period, 20 healthy examinees who matched the age, gender, and education years of the patients were included as the control group. All the research subjects underwent head nuclear magnetic scan plus DTI examination, and the DTI dispersion indicator levels of ipsilateral arcuate fasciculus were compared among different lesions as well as the MoCA scale scores between the MCI group and the non-MCI group. Results　In the MCI group, when the number of lesions was N<2, the FA level and MoCA scale scores of the left arcuate fasciculus were higher than those when 2≤N<4 and N≥4, while the MoCA scale scores of the right arcuate fasciculus were higher than those when 2≤N<4 and N≥4; when the number of lesions was 2≤N<4, the MoCA scale scores of the left and right arcuate fasciculus were higher than those when N≥4, and the differences were statistically significant(P<0.05). In the non-MCI group, when the number of lesions was N<2, the FA levels of the left and right arcuate fasciculus were higher than those when N≥4, while the MD levels were lower than those when N≥4, and the MoCA scale scores of the left arcuate fasciculus were higher than those when 2≤N<4 and N≥4, while the MoCA scale scores of the right arcuate fasciculus were higher than those when N≥4; when the number of lesions was 2≤N<4, the MD levels of the left and right arcuate fasciculus were lower than those when N≥4, and the differences were statistically significant(P<0.05). Conclusion　The changes in microstructure of arcuate fasciculus in patients with lacunar cerebral infarction are associated with MCI. The more lesions in lacunar cerebral infarction, the lower the FA level, and the more severe arcuate fasciculus injury and the lower MoCA scale scores. For the patients with arcuate fasciculus lacunar cerebral infarction in the walking area, head nuclear magnetic scan plus DTI examination can be used as important methods to evaluate MCI and prevent Alzheimer′s disease(AD) by examining the changes in microstructure of arcuate fasciculus and combining them with MoCA scale score.]]> 2024/2/29 0:00:00 1,2, ZHANG Jian¹]]> HAN Songlin^1,2, ZHANG Jian¹ 215true ［Abstract］　Objective　To explore the application value of minimally invasive fascial closure device in pediatric thoracoscopic diaphragm surgery. Methods　The clinical data of 7 cases of diaphragmatic hernia and 1 case of diaphragmatic distension who were admitted to Zhangzhou Affiliated Hospital of Fujian Medical University from January 2019 to November 2022 were retrospectively analyzed. A minimally invasive fascial closure device was used to assist the patients with diaphragmatic hernia repair or diaphragmatic folding under thoracoscope. Results　The surgical process went smoothly, and all the patients were placed with thoracic drainage tube after surgery. Four cases of neonates needed to be assisted by ventilators after surgery, and 3 cases of the four neonates had their ventilators removed on the 2nd, 4th and 5th day after surgery, and 1 case of the four neonates with chromosomal abnormalities developed recurrent pneumothorax and wheezing on the third day after removal of the thoracic drainage tube and tracheal intubation. The tubes were re-inserted, and pulmonary hemorrhage occurred on the 21st day after surgery. On the 24th day after surgery, the patient was voluntarily discharged at the request of the patient′s family members. Except for 1 case who was discharged voluntarily, the other 7 cases had their thoracic drainage tubes removed from 2 days to 11 days after surgery, with an average of 4.4 days. Among the 7 cases, 2 cases had their thoracic drainage tubes removed more than 7 days(9 and 11 days, respectively). The length of postoperative hospitalization was 8-17 days, with an average of 11.8 days. Chest X-rays were re-examined before discharge, and abnormal results were not found. The patients were followed up for 6-12 months after surgery, and no recurrence was observed on the re-examined chest X-rays. Conclusion　It is safe and effective to use minimally invasive fascial closure device assisted under thoracoscope to repair diaphragmatic hernia or diaphragmatic folding in pediatric patients.]]> 2024/2/29 0:00:00 JIAN Ziwei, WANG Bingjie, CHEN Wenyou, CHEN Jinrong 214true ［Abstract］　Objective　To compare the application effects of superficial cervical plexus block combined with cervical 5 nerve root block and superficial cervical plexus block combined with sternoclavicular fascia plane block in internal fixation of clavicular fractures. Methods　A total of 60 patients with unilateral midclavicular fractures who underwent elective internal fixation of clavicular fractures in Beijing Friendship Hospital, Capital Medical University from March 2021 to December 2023 were recruited. The patients were divided into observation group(receiving superficial cervical plexus block combined with cervical 5 nerve root block) and control group(receiving superficial cervical plexus block combined with sternoclavicular fascia plane block) by using random number table method, with 30 cases in each group. The operation time, intraoperative blood loss, sufentanil and propofol dosages, as well as the effectiveness of nerve block, intraoperative heart rate(HR), mean arterial pressure(MAP) level, postoperative Visual Analogue Scale(VAS) scores, modified Bromage Scale(MBS) scores for upper limb motor function after nerve block, and incidence of complications were compared between the two groups. Results　The intraoperative dosages of sufentanil and propofol in the observation group were lower than those in the control group, and the difference was statistically significant(P<0.05). There were no statistically significant differences between the two groups in terms of the operation time, intraoperative blood loss, and the time of first oral analgesic medication after operation(P>0.05). The levels of HR and MAP at different time points during operation in the two groups kept stable, and there were no statistically significant differences between the groups(P>0.05). The effectivenesses of nerve block were good in all the patients. The VAS scores in both groups showed a trend of first decreasing and then increasing at 30 minutes, 4 hours, 12 hours and 24 hours after operation. At all the time points, the VAS scores in the observation group were significantly lower than those in the control group(P<0.05). The MBS scores at the state of elbow flexion, wrist flexion, and finger flexion in both groups showed an upward trend at 30 minutes after nerve block and 4 hours, 12 hours and 24 hours after operation, and there were no statistically significant differences between the groups(P>0.05). One case of mild Horner syndrome and 1 case of partial diaphragmatic paralysis occurred in the observation group after operation, while no complications occurred in the control group. Conclusion　Compared with those in superficial cervical plexus block combined with sternoclavicular fascia plane block, the intraoperative dosages of sufentanil and propofol in superficial cervical plexus block combined with cervical 5 nerve root block are lower. However, both anesthesia methods can better preserve the motor function of the affected upper limbs, and the incidence rates of complications such as phrenic nerve block and recurrent laryngeal nerve block are low.]]> 2025/1/6 0:00:00 LI Peihan, WANG Xuejuan, LI Chengwen, LI Li 213true ［Abstract］　Objective　To investigate the current situation on insomnia in elderly inpatients in several hospitals in Yunnan Province and to analyze the influencing factors through the self-developed comprehensive geriatric assessment system. Methods　The comprehensive geriatric assessment data of 1 635 patients admitted to hospitals at all levels in Yunnan Province from September 2021 to February 2024 were derived from the comprehensive geriatric assessment system. According to insomnia status, the patients were divided into non-insomnia group(556 cases), latent insomnia group(596 cases) and insomnia group(483 cases). The contents of comprehensive geriatric assessment were compared among all the groups, and the influencing factors of insomnia in the elderly inpatients were analyzed by using multivariate logistic regression. Results　There were statistically significant differences in activities of daily living, anxiety status, depression status, cognitive function, nutritional status, urinary incontinence, constipation, family care index, social support status, delirium, fall risk, chronic pain, gait and balance function, and frail condition among the three groups(P<0.05). Mild impairment of daily living ability, probable anxiety, definite anxiety, definite depression, malnutrition, mild-moderate family dysfunction, delirium and fall risk were independent risk factors for the occurrence of insomnia in the elderly inpatients(P<0.05). Conclusion　Mild impairment of daily living ability, probable anxiety, definite anxiety, definite depression, malnutrition, mild-moderate family dysfunction, delirium and fall risk are independent risk factors for the occurrence of insomnia in the elderly inpatients. It is suggested to strengthen the screening and management of the above factors.]]> 2025/1/6 10:33:01 1,3, LI Yan², HUANG Hong², LI Jie⁴, DING Yinju¹, XIAO Fei¹, YI Dan¹, HUANG Fang¹]]> DAI Jingrong^1,3, LI Yan², HUANG Hong², LI Jie⁴, DING Yinju¹, XIAO Fei¹, YI Dan¹, HUANG Fang¹ 212true ［Abstract］　Objective　To explore the effect and acting mechanism of ulinastatin on postoperative delirium(POD) in elderly patients undergoing total hip arthroplasty(THA). Methods　A total of 192 elderly patients undergoing elective surgery of THA were recruited in the Fifth Clinical Medical College of Henan University of Chinese Medicine(People′s Hospital of Zhengzhou) from August 2022 to December 2023. The patients were divided into ulinastatin group(group U) and normal saline group(group N) by using random number table method, with 96 cases in each group. In group U, 5 000 U/kg of ulinastatin was injected intravenously 10 minutes before anesthesia induction until the end of surgery, and in group N, the same volume of normal saline was injected intravenously until the end of surgery. The patients were assessed for POD by using confusion assessment method-intensive care unit(CAM-ICU) and Richmond Agitation-Sedation Scale(RASS) within 3 days after surgery. Blood samples were collected 48 hours after surgery, and peripheral blood mononuclear cells(PBMCs) were isolated. The concentration of ferric ion(Fe²⁺), the content of malondialdehyde(MDA) and the activity of superoxide dismutase(SOD) in PBMCs were determined by using the colorimetric method. The expression levels of long-chain acyl-CoA synthetase 4(ACSL4) and glutathione peroxidase 4(GPX4) were determined by using Western blot method. The clinical data of the patients were collected. Results　In group U, the operation of 1 patient was canceled before surgery, and 1 patient underwent a second operation to stop bleeding after surgery, and 94 patients were finally included. In group N, 1 patient voluntarily dropped out of the study after surgery, 2 patients underwent a second operation to stop bleeding after surgery, and 93 patients were finally included. Within 3 days after surgery, the incidence of POD in group U was significantly lower than that in group N(20.21% vs 35.48%; χ²=5.431, P=0.020). There were no significant differences in POD severity scores and POD duration between the two groups(P>0.05). Compared with that in group N, the length of postoperative hospitalization in group U was significantly shortened(P<0.05). There were no significant differences in operation time, total anesthesia time, blood loss volume, fluid intake and the duration of stay in postanesthesia care unit(PACU) between the two groups(P>0.05). Compared with group N, group U had a decrease in Fe²⁺ concentration and MDA content, an increase in SOD activity, a decrease in the expression of ACSL4 protein, and an increase in the expression of GPX4 protein in PBMCs 48 hours after surgery, and the differences were statistically significant(P<0.05). There was no significant difference in the incidence of adverse events between the two groups(P>0.05). Conclusion　Ulinastatin has a neuroprotective effect, which can improve neurological dysfunction and reduce the incidence of POD in elderly patients undergoing THA. The mechanism of action may be related to inhibiting ferroptosis in PBMCs and anti-oxidative stress.]]> 2025/1/6 0:00:00 ZHANG Xungong, WU Xiaoshun 211true ［Abstract］　Objective　To explore the effect of bacterial lysates on treatment of childhood allergic rhinitis and the regulating effect of the bacterial lysates on serum interferon-γ(IFN-γ) and interleukin-4(IL-4) levels. Methods　Sixty pediatric patients with allergic rhinitis who were admitted to Department of Otolaryngology, Affiliated Hospital of Zunyi Medical University from January 2023 to January 2024 were recruited and divided into observation group and control group by using random number table method, with 30 cases in each group. The control group was treated with mometasone furoate combined with montelukast sodium, and the observation group was treated with bacterial lysates on the basis of the treatment regimen of the control group. Both groups were treated continuously for 3 months. Serum IFN-γ, IL-4 and immunoglobulin E(IgE) levels, and symptom and sign scores were compared between the two groups before and after treatment, and clinical efficacy and adverse reactions were compared between the two groups. Results　Before treatment, there were no significant differences in serum IFN-γ, IL-4 and IgE levels between the two groups(P>0.05). After treatment, serum INF-γ levels in both groups were significantly increased compared with those before treatment(P<0.05), while serum IL-4 and IgE levels in both groups were significantly decreased compared with those before treatment(P<0.05), and the level changes in the observation group were more significant than those in the control group(P<0.05). Before treatment, there were no significant differences in the symptom scores of runny nose, nasal congestion, nasal itching and sneezing, and the sign scores of nasal mucosal swelling and inferior turbinate edema between the two groups(P>0.05). After treatment, the scores of various symptoms and signs in both groups decreased compared with those before treatment, and the scores in the observation group were lower than those in the control group, and the differences were statistically significant(P<0.05). The total effective rate of the observation group was higher than that of the control group, and the difference was statistically significant(96.67% vs 73.33%; χ²=4.706, P=0.030). There was no significant difference in the incidence of adverse reactions between the two groups(20.00% vs 23.33%; χ²=0.098, P=0.754). Conclusion　Bacterial lysates are helpful for improving the clinical treatment effect of childhood allergic rhinitis, increasing serum INF-γ level, reducing serum IL-4 and IgE levels and improving clinical symptoms, and have a good safety.]]> 2025/1/6 10:33:01 XIE Tianhong, ZHU Wenya, WANG Shifei 210true ［Abstract］　Objective　To explore the effects of cardiopulmonary rehabilitation training combined with enhanced external counterpulsation(EECP) on cardiopulmonary reserve function, vascular endothelial function and athletic ability in chronic obstructive pulmonary disease(COPD) patients complicated with non-high-risk stable angina pectoris. Methods　A total of 80 COPD patients complicated with non-high-risk stable angina pectoris who were admitted to Beijing Rehabilitation Hospital Affiliated to Capital Medical University from January 2020 to June 2022 were selected and randomly divided into control group(n=40) and observation group(n=40). The control group received basic treatment and cardiopulmonary rehabilitation training, while the observation group was supplemented with EECP intervention on the basis of the intervention in the control group. Both groups were treated for 5 weeks. The cardiopulmonary reserve function, vascular endothelial function, athletic ability, angina attack and quality of life were compared between the two groups. Results　After intervention, forced expiratory volume in 1 second/forced vital capacity(FEV₁/FVC), maximal voluntary ventilation(MVV) and left ventricular ejection fraction(LVEF) in both groups were significantly elevated(P<0.05), and these indicators in the observation group were higher than those in the control group(P<0.05). After intervention, the levels of nitric oxide(NO) were significantly increased(P<0.05), while the levels of endothelin-1(ET-1) and endothelial cell-specific molecule-1(ESM-1) were significantly decreased in both groups(P<0.05), and the level of NO in the observation group was significantly higher than that in the control group(P<0.05), and the levels of ET-1 and ESM-1 in the observation group were significantly lower than those in the control group(P<0.05). After intervention, the total exercise time, the exercise tolerance and the spending time of ST segment depression by 0.1 mV were elevated, while the downward amplitude of ST segment was significantly decreased in both groups(P<0.05), and the total exercise time, the exercise tolerance and the spending time of ST segment depression by 0.1 mV in the observation group were significantly higher than those in the control group(P<0.05), and the downward amplitude of ST segment in the observation group was lower than that in the control group(P<0.05). After intervention, the frequency and intensity of angina attacks were significantly decreased(P<0.05), and the scores of Seattle Angina Questionnaire(SAQ) scale were significantly increased in both groups(P<0.05), and the frequency and intensity of angina attacks in the observation group were significantly lower than those in the control group(P<0.05), and the SAQ scores in the observation group were significantly higher than those in the control group(P<0.05). After intervention, the scores of World Health Organization Quality of Life-BREF(WHOQOL-BREF) in physiological and psychological fields were significantly decreased in both groups(P<0.05), and the scores of WHOQOL-BREF in the observation group were significantly higher than those in the control group(P<0.05), but there was no significant change in the scores of environmental and social relations fields(P>0.05). Conclusion　Cardiopulmonary rehabilitation training combined with EECP can effectively improve cardiopulmonary reserve function, vascular endothelial function and athletic ability in COPD patients complicated with non-high-risk stable angina pectoris, and alleviate angina attack and improve quality of life.]]> 2025/1/6 10:33:01 SUN Xiaojing, WANG Lizhong, ZHANG Zhenying, ZHANG Liu, XU Zhiping, YANG Cheng 209true ［Abstract］　Objective　To construct a random forest model for the diagnosis of chronic kidney disease(CKD) based on retinal blood flow density indicators to promote the early diagnosis and personalized prevention and treatment of CKD. Methods　Twenty-seven patients with CKD(44 eyes) who were admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region from February 2022 to December 2023 were recruited as the case group, and 47 healthy people(71 eyes) were recruited as the control group during the same period. Retinal blood flow density indicators were obtained by using optical coherence tomography angiography(OCTA). A random forest model used for diagnosing CKD was constructed based on retinal blood flow density indicators and general clinical indicators, and the interpretability of the model was provided by Shapley importance analysis and Shapley method. Results　The whole superficial capillary plexus(SCP) blood flow density and the lower half SCP blood flow density in the case group were higher than those in the control group, and the differences were statistically significant(P<0.05), while there were no statistically significant differences in the other indicators of retinal blood flow density between the two groups(P>0.05). The accuracy of the model was 86.96%, and the sensitivity of the model was 100.00%, and the specificity of the model was 72.73%. The upper half SCP blood flow density, the whole SCP blood flow density, Early Treatment Diabetic Retinopathy Study(ETDRS) region of the SCP blood flow density, the lower half SCP blood flow density, the whole deep capillary plexus(DCP) blood flow density, and the upper half DCP blood flow density were the main contributory indicators in the predictive model. Conclusion　The random forest model based on retinal blood flow density indicators can effectively assist the diagnosis of CKD.]]> 2025/1/6 10:33:01 1, LEI Daizai², YANG Yingxia³, HONG Yiyi², HU Xiangyu^1,2, YE Kun⁴, XU Fan^1,2]]> LAN Lin¹, LEI Daizai², YANG Yingxia³, HONG Yiyi², HU Xiangyu^1,2, YE Kun⁴, XU Fan^1,2 208true ［Abstract］　Objective　To compare the clinical effects of different surgical methods for treating perennial allergic rhinitis(AR) with nasal anatomical variations and(or) inflammation. Methods　The clinical data of 150 patients suffering from perennial AR with nasal anatomical variations and(or) inflammation who were admitted to Donghui Hospital of Guigang City and Jixi People′s Hospital from December 2021 to August 2023 were retrospectively analyzed. According to different surgical methods, the patients were divided into control group(receiving submucosal inferior turbinate bone resection or septoplasty+submucosal inferior turbinate bone resection, 75 cases) and observation group(receiving selective vidian nerve transection+submucosal inferior turbinate bone resection, or selective vidian nerve transection+septoplasty+submucosal inferior turbinate bone resection, 75 cases). Visual Analogue Scale(VAS) scores were used to evaluate the nasal symptoms of the patients in the two groups before surgery and at 6 and 12 months after surgery. The clinical effects and the incidence of postoperative complications were compared between the two groups at 6 and 12 months after surgery. Results　The VAS scores for postoperative nasal congestion, nasal itching, runny nose, and sneezing in both groups showed a decreasing trend, and the reductions of these VAS scores in the observation group were greater than those in the control group(P<0.05). The VAS scores for nasal congestion, nasal itching, runny nose and sneezing in the observation group were lower than those in the control group at 6 and 12 months after surgery, and the differences were statistically significant(P<0.05). The clinical effects in the two groups were equivalent at 6 months after surgery(P>0.05), while the total clinical effective rate of the observation group at 12 months after surgery was significantly higher than that of the control group(97.33% vs 72.00%; χ²=18.538, P<0.001). In the observation group, there was 1 case of palatal numbness, 3 cases of headache, and no cases of dry eye syndrome and nasal bleeding. There were no surgical-related complications in the control group.There was no statistically significant difference in the incidence of complications between the two groups(5.33% vs 0.00%; P=0.056). Conclusion　Selective vidian nerve transection+submucosal inferior turbinate bone resection, or selective vidian nerve transection+septoplasty+submucosal inferior turbinate bone resection has significant clinical efficacy and good safety in treatment of perennial AR, and is worthy of clinical recommendation.]]> 2025/1/6 10:33:01 1, MA Jia², QI Sen¹, WANG Xu¹, GAN Jinxia¹, SUN Lu¹, MENG Baili¹]]> XU Junfang¹, MA Jia², QI Sen¹, WANG Xu¹, GAN Jinxia¹, SUN Lu¹, MENG Baili¹ 207true ［Abstract］　Objective　To explore the acting mechanism of patient-derived organoids(PDO)-pancreatic cancer primary tumor-derived immune cells based on single-cell ribonucleic acid(RNA) sequencing. Methods　Single-cell RNA sequencing datasets of pancreatic cancer was obtained from the Genome Expression Omnibus(GEO) database, and the relevant clinical data of 185 cases of pancreatic cancer patients were downloaded from the Cancer Genome Atlas(TCGA) database. After quality control, dimensionality reduction, clustering and cellular annotation, the differences in the composition and distribution of immune cells on pancreatic cancer primary tumors and PDO were analyzed, and the differentially expressed genes(DEGs) of immune cells were screened. Molecular interactions between immune cells were analyzed using CellChat, and the immune cell communication patterns were recognized. Subsequently, Gene Ontology(GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis were performed for neutrophils DEGs. Finally, the effects of expression levels of FFAR2 and RASGEF1C genes on survival prognosis of the patients were analyzed. Results　In this study, 22 cell populations were successfully identified and further categorized into 11 types of different cells, among which there were 4 types of immune cells, including neutrophils. The single-cell RNA sequencing data of immune cells were extracted to obtain 30 141 cells, including 2 282 cells from PDO model and 7 319 cells from primary tumors. There were significant differences in the composition and number of immune cells in the samples from different sources. The molecular interactions between immune cells and the communication patterns of immune cells were analyzed through CellChat, and the transforming growth factor-beta(TGF-β) signaling pathway and neutrophils were identified as playing an important role in the development of pancreatic cancer. In addition, the study found that neutrophils could activate TGF-β and other signaling patterns and pathways at the same time. DEGs of neutrophils were mainly involved in biological processes such as regulation of immunoreaction, activation of immune response transport, activation of innate immune response, and were enriched in signaling pathways such as primary immune deficiency and asthma. The less studied FFAR2 and RASGEF1C genes in neutrophil DEGs were selected for survival analysis, and the results suggested that the high expressions of FFAR2 and RASGEF1C were helpful to improve the survival prognosis of the pancreatic cancer patients. Conclusion　This study confirms the difference of immune cells between primary tumors and PDO in pancreatic cancer, and lays the foundation for studies on organoid in pancreatic cancer.]]> 2025/1/6 10:33:01 1, LU Heming², CAO Tingting¹]]> XU Yingcui¹, LU Heming², CAO Tingting¹ 206true ［Abstract］　Objective　To analyse the value of osteopontin(OPN) in nasal secretions in diagnosis of eosinophilic chronic rhinosinusitis with nasal polyps(CRSwNP). Methods　A total of 80 patients with CRSwNP who underwent endoscopic sinus surgery and 30 patients who underwent nasal septoplasty(control group) in Renmin Hospital of Wuhan University from January to June 2023 were recruited. The CRSwNP patients were divided into eosinophilic CRSwNP(eCRSwNP) group(eosinophils to subepithelial infiltrating immune cells ratio≥10%) and non-eosinophilic CRSwNP(neCRSwNP) group(eosinophils to subepithelial infiltrating immune cells ratio<10%) according to eosinophilic granulocyte count in tissues, with 40 cases in each group. OPN levels in the patients′ nasal polyp/turbinate tissues and nasal secretions were detected, and the clinical data of the CRSwNP patients were collected, and the correlation between the OPN level in nasal secretions and the clinical feature was investigated by using Pearson correlation analysis. Receiver operating characteristic(ROC) curve was used to analyze the diagnostic value of OPN in eCRSwNP. Results　Compared to the control group, both the eCRSwNP group and the neCRSwNP group exhibited significantly elevated levels of OPN in nasal polyp tissues and nasal secretions, and the eCRSwNP group had higher OPN levels than the neCRSwNP group, and the differences were statistically significant(P<0.05). The OPN levels in the CRSwNP patients′ nasal secretions were positively correlated with peripheral blood eosinophil count(r=0.715, P<0.001), percentage of peripheral blood eosinophils(r=0.571, P<0.001), eosinophil count in nasal polyp tissues(r=0.521, P<0.001), Lund-Mackay scores(r=0.421, P=0.006) and Lund-Kennedy scores(r=0.386, P=0.013). The eCRSwNP group had higher peripheral blood eosinophil count, percentage of peripheral blood eosinophils, Lund-Mackay scores and Lund-Kennedy scores than the neCRSwNP group, and the differences were statistically significant(P<0.05). However, there were no significant differences in serum total IgE, nasal fractional exhaled nitric oxide(FnNO), and Visual Analogue Scale(VAS) scores between the two groups(P>0.05). The results of ROC curve analysis indicated that the OPN level in nasal secretions, peripheral blood eosinophil count, percentage of peripheral blood eosinophils, Lund-Mackay scores and Lund-Kennedy scores were helpful in the diagnosis of eCRSwNP, and the diagnostic efficiency of the OPN level in nasal secretions was higher［AUC(95%CI)=0.837(0.763-0.997), P<0.001］, with a sensitivity of 95.42% and a specificity of 65.37%. Conclusion　The OPN level in nasal secretions can be used as a biomarker in the diagnosis of eCRSwNP.]]> 2024/12/12 0:00:00 LIU Peiqiang, XU Yu 205true ［Abstract］　Objective　To observe the efficacy of posterior atlantoaxial screw rod reduction and internal fixation combined with transplantation of the muscle-vessel complex of the atlantoaxial spinous process in treatment of type ⅡC odontoid fractures. Methods　The clinical data of 95 patients with type ⅡC odontoid fractures who were admitted to the 910th Hospital of Joint Logistic Force of Chinese People′s Liberation Army from January 2020 to October 2022 were retrospectively analyzed. The patients were divided into control group(receiving posterior atlantoaxial screw rod reduction and internal fixation combined with iliac bone grafting, 47 cases), and observation group(receiving posterior atlantoaxial screw rod reduction and internal fixation combined with transplantation of the muscle-vascular complex of the atlantoaxial spinous process, 48 cases). The operation-related indicators, postoperative fracture healing rate, fracture end and neck stability and atlantoaxial rotation degree were compared between the two groups. Visual Analogue Scale(VAS) scores, American Spinal Injury Association(ASIA) scores, neck disability index(NDI) and Japanese Orthopaedic Association(JOA) scores were compared between the two groups before operation, 3 months after operation and 6 months after operation. The complications were compared between the two groups. Results　Compared with the control group, the observation group had shorter operation time, less blood loss during operation, and shorter time to fracture healing, and the differences were statistically significant(P<0.05). The fracture healing rate in the observation group was significantly higher than that in the control group 6 months after operation［93.75%(45/48) vs 78.72%(37/47); χ²=4.540, P=0.033］. Six months after operation, dynamic X-ray films of cervical flexion and extension showed good stability of the fracture ends and necks in all the patients in the observation group, while four patients in the control group had poor stability of the fracture ends. There was no significant difference in the atlantoaxial rotation degree between the observation group and the control group 3 months after fracture healing［(84.13±6.32)°vs (82.96±7.10)°; t=0.849, P=0.398］. The postoperative VAS scores showed a downward trend in both groups(P<0.05), but there was no significant difference in the magnitude of the change between the groups(P>0.05). Compared with those before operation, the ASIA scores 3 and 6 months after operation did not change significantly in both groups(P>0.05), and NDI showed a downward trend in both groups after operation(P<0.05), and NDI scores in the observation group was significantly lower than that in the control group 3 and 6 months after operation(P<0.05). The JOA scores showed an upward trend after operation in both groups(P<0.05), and the JOA scores in the observation group were significantly higher than those in the control group 3 and 6 months after operation(P<0.05). The incidence of complications in the observation group was lower than that in the control group, but the difference was not statistically significant between the two groups［4.17%(2/48) vs 14.89%(7/47); χ²=2.058, P=0.151］. Conclusion　The posterior atlantoaxial screw rod reduction and internal fixation combined with transplantation of the muscle-vascular complex of the atlantoaxial spinous process can shorten the operation time, reduce intraoperative blood loss, promote fracture healing and facilitate the recovery of physiological function of cervical vertebrae in the treatment of type ⅡC odontoid fractures.]]> 2024/12/12 8:08:13 HONG Qingnan, ZHENG Gengyang, LI Da, LU Yizhe, ZHAO Feng 204true ［Abstract］　Objective　To analyze the expressions of long non-coding RNA(lncRNA) ribonuclease P RNA component H1(RPPH1) and anterior gradient protein 3(AGR3) in colorectal cancer(CRC) tissues and their clinical significance. Methods　A total of 98 patients with CRC who were admitted to XD Group Hospital from January 2017 to January 2020 were recruited. During the operation, CRC tissues and their paracancer tissues(at a distance of more than 3 cm from the edge of the cancer tissue, and no cancer cells being found by pathological examination) were collected. The expression levels of lncRNA RPPH1 were detected by using real-time quantitative polymerase chain reaction(PCR). The expression of AGR3 was detected by using immunohistochemical staining. The correlations of the expressions of lncRNA RPPH1 and AGR3 in the CRC tissues with the patients′ clinicopathological features and survival prognosis were analyzed. Results　The expression level of lncRNA RPPH1 in the CRC tissues was significantly higher than that in the paracancer tissues(P<0.05), and the positive rate of AGR3 in the CRC tissues was significantly higher than that in the paracancer tissues［65.31%(64/98) vs 10.20%(10/98), P<0.001］. The expressions of lncRNA RPPH1 and AGR3 in the CRC tissues were significantly correlated with tumor TNM stage, tumor differentiation degree and lymph node metastasis(P<0.05). The survival prognosis of the low lcRNA RPPH1 expression group was significantly better than that of the high lcRNA RPPH1 expression group(log-rank test: χ²=9.222, P=0.002). The survival prognosis of the AGR3-negative group was significantly better than that of the AGR3-positive group(log-rank test: χ²=8.090, P=0.005). The results of multivariate Cox regression analysis showed that lymph node metastasis, tumor TNM stage Ⅲ, high expression of lncRNA RPPH1 and AGR3 positive were independent risk factors of poor survival prognosis for the CRC patients(P<0.05). Conclusion　The increased expression level of lncRNA RPPH1 and positive expression of AGR3 in CRC tissues are associated with adverse clinicopathological features and poor prognosis of the patients.]]> 2024/12/12 8:08:13 PING Sheng, MOU Yagang, CHENG Xuan, LI Xiang, ZHUANG Yunlong 203true ［Abstract］　Objective　To analyze the expression of differentially expressed miRNA in osteosarcoma(OS) patients and its correlation with survival prognosis. Methods　The bioinformatics analysis method was used to identify differentially expressed miRNA in GSE69470 and GSE70367 datasets. Sixty-three OS patients who visited their doctors in Zhang Ye People′s Hospital Affiliated to Hexi University from August 2018 to January 2019 were recruited. Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR) was used to detect the expression levels of differentially expressed miRNA in OS tissues and their paracancer tissues, and the expression level of differentially expressed miRNA in the OS tissues was compared with that in the paracancer tissues. The patients were followed up by telephone or outpatient reexamination methods and their survival situation was recorded. The patients were followed up for 5 years until January 2024. The Kaplan-Meier method was used to analyze the correlation between the differentially expressed miRNA expression in OS tissues and the survival prognosis of the OS patients. Results　Eight differentially expressed miRNA were obtained by bioinformatics analysis, including microRNA(miR)-543, miR-495-3p, miR-433-3p, miR-381-3p, miR-301a-3p, miR-199b-5p, miR-125b-5p and miR-218. The results of RT-qPCR showed that the relative expression levels of miR-543, miR-495-3p and miR-125b-5p in the OS tissues were lower than those in the paracancer tissues, while the relative expression levels of miR-433-3p, miR-381-3p, miR-301a-3p and miR-199b-5p in the OS tissues were higher than those in the paracancer tissues, and the differences were statistically significant(P<0.05). There was no statistically significant difference in the expression level of miR-218 between the OS tissues and the paracancer tissues(P>0.05). The patients were grouped according to the median relative expression of each miRNA: 31 cases in the high expression group(>median value) and 32 cases in the low expression group(≤median value).The analysis results of Kaplan-Meier method showed that the survival prognosis in the miR-543 high expression group, the miR-495-3p high expression group and the miR-125b-5p high expression group were better than that in the miR-543 low expression group, the miR-495-3p low expression group and the miR-125b-5p low expression group, respectively, and that the survival prognosis in the miR-433-3p low expression group, the miR-381-3p low expression group, the miR-301a-3p low expression group and the miR-199b-5p low expression group was better than that in the miR-433-3p high expression group, the miR-381-3p high expression group, the miR-301a-3p high expression group and the miR-199b-5p high expression group, respectively, and the differences were statistically significant(P<0.05). Conclusion　The relative expression levels of miR-543, miR-495-3p and miR-125b-5p in the OS tissues are lower than those in the paracancer tissues, while the relative expression levels of miR-433-3p, miR-381-3p, miR-301a-3p and miR-199b-5p in the OS tissues are higher than those in the paracancer tissues. The above 7 kinds of differentially expressed miRNA are associated with the survival prognosis of the OS patients.]]> 2024/12/12 8:08:13 LIU Qi, QIAN Jun, CAO Juan, YANG Qian 202true ［Abstract］　Objective　To explore the differences in therapeutic effects of cognitive behavioral therapy for insomnia(CBTI) in chronic insomnia disorder patients with different personality traits. Methods　Sixty patients with chronic insomnia disorder who were admitted to the Second People′s Hospital of Guizhou Province from January 2018 to August 2021 were recruited. According to the results of the Eysenck Personality Questionnaire(EPQ) revised by Gong Yaoxian, the patients were divided into neuroticism group, extraversion group and psychoticism group, with 20 cases in each group. All the three groups received CBTI for 8 weeks. Sleep diaries were used to assess the changes in subjective and objective sleep quality indicators in the patients of the three groups before treatment(T₀), at the end of treatment(T₁), and three months after the end of treatment(T₂). The relevant indicators of polysomnography(PSG) and serum cortisol levels were compared among the three groups at the time points of T₀ and T₁. Results　According to the sleep diaries, sleep latency(SL), wake after sleep onset(WASO), sleep efficiency(SE) and total sleep time(TST) in the neuroticism group after treatment did not change significantly compared with those before treatment(P>0.05). After treatment, SL and WASO gradually decreased, while SE and TST gradually increased in the extraversion group and the psychoticism group, and there were significant differences compared with those at the time point of T₀(P<0.05). Compared with the neuroticism group, the extraversion group and the psychoticism group had shorter ST, longer TST and higher SE at T₁ and T₂ time points, and the differences were statistically significant(P<0.05). However, there was no statistically significant difference in each indicator between the extraversion group and the psychoticism group(P>0.05). According to the PSG results, there was no significant difference in the change of each indicator in the neuroticism group before and after treatment(P>0.05). In the extraversion group, compared with those at the time point of T₀, WASO and the proportion of N2 stage decreased, while SE and the proportion of N3 stage, TST and the proportion of rapid eye movement(REM) stage increased significantly at the time point of T₁(P<0.05). In the psychoticism group, comopared with those at the time point of T₀, TST, SE and the proportion of N3 stage increased, and WASO decreased at the time point of T₁, and the differences were statistically significant(P<0.05). The results of inter-group comparison showed that there were no statistically significant differences in SL, WASO, TST, SE, and proportions of N1 stage, N2 stage and N3 stage among the three groups at the time point of T₀(P>0.05). At the time point of T₁, compared with the neuroticism group, the extraversion group had a decrease in the proportion of N2 stage and an increase in the proportion of REM, and compared with the neuroticism group, the psychoticism group had decreases in WASO and the proportion of N2 stage and increases in SE and the proportion of N3 stage, and the differences were significant(P<0.05). Compared with that at the time point of T₀, the serum cortisol level in the neuroticism group increased while the serum cortisol level in the psychoticism group decreased at the time point of T₁, and the differences were significant(P<0.05), but the serum cortisol level of the extraversion group after treatment did not change significantly compared with that before treatment(P>0.05). The results of inter-group comparison showed that the serum cortisol levels in the three groups did not change significantly compared with those before treatment(P>0.05). Conclusion　There are significant differences in therapeutic effects of CBTI in chronic insomnia disorder patients with different personality traits. The effects of CBTI in the insomnia patients with psychopathic personality and extraverted personality are better, but the effect of CBTI in the insomnia patients with neurotic personality is unsatisfactory.]]> 2024/12/12 8:08:13 1, WANG Ying¹, FANG Chao¹, SHANG Yuanhao², WANG Ziyun², HU Jin²]]> FENG Xia¹, WANG Ying¹, FANG Chao¹, SHANG Yuanhao², WANG Ziyun², HU Jin² 201true ［Abstract］　Objective　To explore the effect of hyperbaric oxygen combined with Yangxue Jieyu Xingnao Decoction on sleep quality of patients with sleep disorders after acute cerebral infarction. Methods　A total of 160 patients with sleep disorders after acute cerebral infarction who were admitted to Hai′an People′s Hospital from January 2022 to December 2022 were recruited. The patients were divided into control group and observation group by random number table method, with 80 cases in each group. The control group was given hyperbaric oxygen treatment, and the observation group was given hyperbaric oxygen combined with Yangxue Jieyu Xingnao Decoction treatment. Both groups were given continuous treatment for 4 weeks, and the therapeutic efficacy was compared between the two groups after the end of treatment. Results　The Pittsburgh Sleep Quality Index(PSQI) rating scale scores and Epworth Sleepiness Scale(ESS) scores after treatment were lower than those before treatment in the two groups, and the PSQI scores and ESS scores in the observation group were lower than those in the control group after treatment, and the total sleep time(TST) and rapid eye movement sleep time(RT) after treatment was longer than that before treatment in the two groups, and the sleep latency(SL) after treatment was shorter than that before treatment in the two groups, and the TST and RT in the observation group was longer than that in the control group, and the SL in the observation group was shorter than that in the control group after treatment. The serum levels of norepinephrine(NE) and 5-hydroxytryptamine(5-HT) after treatment were higher than those before treatment in the two groups, and the serum levels of NE and 5-HT in the observation group were higher than those in the control group after treatment, and the differences were statistically significant(P<0.05). The total effective rate of the observation group was higher than that of the control group(93.75% vs 78.75%; χ²=7.589, P=0.006). During the treatment, there were no serious adverse reactions such as allergy, liver and kidney injury in both groups. Conclusion　Hyperbaric oxygen combined with Yangxue Jieyu Xingnao Decoction can effectively improve the sleep quality of patients with sleep disorders after acute cerebral infarction, and this combined treatment has good safety.]]> 2024/12/12 8:08:13 1, YANG Xiaoli², LYU Xia¹, JIANG Li¹]]> WU Xiaopei¹, YANG Xiaoli², LYU Xia¹, JIANG Li¹ 200true ［Abstract］　Objective　To examine the pregnant women with pernicious placenta previa(PPP) using uterine arcuate artery ultrasound, and to establish a risk prediction model for postpartum hemorrhage using ultrasound parameters based on logistic regression. Methods　A total of 103 PPP pregnant women who gave birth in the Tenth Affiliated Hospital of Southern Medical University(Dongguan People′s Hospital) from June 2019 to June 2022 were selected and their postpartum hemorrhage was followed up, and the PPP pregnant women were divided into hemorrhage group(The amount of blood lost within the first 24 hours after delivery≥1 000 mL was difined as the occurrence of postpartum hemorrhage) and non-hemorrhage group(The amount of blood lost within the first 24 hours after delivery<1 000 mL was defined as the absence of postpartum hemorrhage) according to the amount of postpartum hemorrhage. The general data and the uterine arcuate artery ultrasound parameters were compared between the two groups, and multivariate logistic analysis was conducted on postpartum hemorrhage, and a risk prediction model was established and evaluated, and the value of the risk prediction model in predicting postpartum hemorrhage was analyzed by plotting receiver operating characteristic(ROC) curve. Results　The peak systolic velocity(PSV) in the hemorrhage group was higher than that in the non-hemorrhage group, and the resistance index(RI) and pulsatility index(PI) in the hemorrhage group were lower than those in the non-hemorrhage group(P<0.05). The uterine arcuate artery ultrasound parameters of PSV,RI and PI were the influencing factors of postpartum hemorrhage(P<0.05). A logistic regression model was established: logit(P)=-40.002+1.386×PSV-0.926×RI-0.783×PI, with good fitting degree. The area under the curve(AUC) for predicting postpartum hemorrhage was 0.860 based on the fitted model, which was larger than that for predicting postpartum hemorrhage by using single indicator of PSV(χ²=2.355, P=0.019), RI(χ²=2.332, P=0.020) and PI(χ²=2.472, P=0.013). Conclusion　The risk prediction model established for postpartum hemorrhage caused by PPP in pregnant women using uterine arcuate artery ultrasound parameters based on logistic regression can effectively determine the risk of postpartum hemorrhage, and the risk prediction model is helpful for early prevention and treatment in clinical practice.]]> 2024/12/12 8:08:13 HUANG Qiaoru, LI Zilin, LIU Yingfeng, YIN Wanping, CHEN Jinghua, HUANG Lishan, ZHOU Qianjun 199true ［Abstract］　Objective　To explore the clinical value of iterative model reconstruction(IMR) combined with low-tube-voltage imaging in evaluating mesenteric artery. Methods　Sixty patients undergoing total abdominal computed tomography(CT) arterial imaging(CTA) examination in the Second Affiliated Hospital of Guangxi Medical University from January 2020 to December 2022 were recruited and divided into control group(n=30) and trial group(n=30) by lottery method. The imaging of the control group was reconstructed by using 120 kV tube voltage combined with iDose⁴. The imaging of the trial group was reconstructed by using 100 kV tube voltage combined with IMR. The CT value, noise value(standard deviation, SD), signal-to-noise ratio(SNR), contrast-to-noise ratio(CNR) of the examined images, and subjective scores of the image quality and the evaluation indicators for radiation dose［volume CT dose index(CTDI_VOL), dose-length product(DLP) and effective radiation dose(ED)］ were compared between the two groups. The subjective scores of the image quality were performed independently by two physicians. Intraclass correlation coeficient(ICC) consistency test was used to evaluate the consistency of the results. Results　The noise value(SD) of the superior mesenteric artery and the noise value(SD) of the inferior mesenteric artery in the trial group were lower than those in the control group, and the CT value, SNR and CNR in the trial group were higher than those in the control group, and the differences were statistically significant(P<0.05). The results of ICC consistency test showed that the image quality scores performed independently by the two physicians in the same group were well consistent(P<0.05). The ultimate subjective scores of image quality in the trial group were significantly higher than those in the control group(P<0.05). The levels of CTDI_VOL, DLP and ED in the trial group were lower than those in the control group, and the differences were statistically significant(P<0.05). Conclusion　IMR combined with low-tube-voltage imaging can improve the image quality of mesenteric artery and reduce ED in the patients, which is worthy of clinical application.]]> 2024/12/12 8:08:13 MENG Yuanyi, QIN Ming′an, YU Xiaoping, XIA Zhenyuan, MO Xinxin, LU Bingfeng 198true ［Abstract］　Objective　To analyze the expression of TSC22D1 in oropharyngeal squamous cell carcinoma(OPSCC) and to explore its correlation with human papilloma virus(HPV) infection(p16+). Methods　The clinical data and tissue specimens of 55 patients with OPSCC were retrospectively analyzed, including 27 cases of tonsil cancer, 25 cases of tongue root cancer and 3 cases of soft palate cancer. The tissue specimens of 12 cases of tongue squamous cell carcinoma(TSCC) were collected for analysis, and 11 cases of specimens of tonsillitis and 2 cases of specimens of paracarcinomatous tongue epithelium were used as controls. Immunohistochemical staining was used to detect the expressions of TSC22D1 and p16. The relationship between the expression of TSC22D1 and the clinicopathological indicators of OPSCC patients was analyzed. Results　TSC22D1 was continuously expressed in the columnar epithelial zone at the opening of Von Ebner′s glandular ducts as well as in the basal layer of the transformation zone in the paracarcinoma tongue, and was only sporadically expressed in basal cells in the epithelial tissues of the paracarcinoma tongue, and was densely expressed in some TSCCs and carcinomas of the tongue root. There was no significant difference in the expression of TSC22D1 between TSCC tissues and the tissues of tongue root carcinomas(P>0.05). TSC22D1 was continuously expressed in the reticular epithelium of tonsils and was positively expressed in some tonsillar carcinomas. The positive expression rate of TSC22D1 in non-cancerous tonsil tissues(tonsillitis tissues) was higher than that in cancerous tonsil tissues, and the difference was statistically significant(P<0.05). The low differentiation rate of TSC22D1-positive OPSCC was significantly higher than that of TSC22D1-negative OPSCC(P<0.05), and the TSC22D1 expression was not significantly associated with the patients′ gender, age, number of cigarettes smoked, ethnicity, site of disease, N stage, T stage, clinical stage and HPV infection(p16+)(P>0.05). Conclusion　TSC22D1 may be a susceptible regional marker of OPSCC, and its expression reduces in carcinogenesis of oropharynx. TSC22D1 may not be significantly associated with the expression of p16.]]> 2024/12/12 8:08:13 1, CHEN Pengning¹, CHEN Xinyu¹, CHEN Guanxi¹, LUO Lin¹, ZHOU Bosen¹, ZHOU Mengyu², RUAN Ping³, YU Dahai²]]> LIU Nengming¹, CHEN Pengning¹, CHEN Xinyu¹, CHEN Guanxi¹, LUO Lin¹, ZHOU Bosen¹, ZHOU Mengyu², RUAN Ping³, YU Dahai² 197true ［Abstract］　Objective　To observe the therapeutic effect of gallbladder-preserving lithotomy in cholelithiasis under transumbilical endoscope and the application effect of absorbable clips in the surgical procedure. Methods　The clinical data of 7 patients with cholelithiasis who underwent gallbladder-preserving lithotomy under transumbilical endoscope in the People′s Hospital of Guangxi Zhuang Autonomous Region from August 2020 to August 2021 were retrospectively analyzed, and their surgical effects were summarized. Results　The operations of the 7 patients were successfully completed, and the operation time was 121-275 minutes. There were 6 cases with intraoperative blood loss ≤10 mL and 1 case with intraoperative blood loss >20 mL. Four to ten absorbable clips were used during the operations. The closure time of gallbladders was 29-93 minutes. The time of first anal exhaust or first defecation after the operations was 24-36 hours. Mild postoperative pain occurred in 2 cases, and the pain could be relieved by itself. The duration of hospitalization was 6-10 days, and no complications such as fever, biliary fistula and poor closure were found during hospitalization. The follow-up evaluations showed good gallbladder function in the patients. Conclusion　The treatment of cholelithiasis with gallbladder-preserving lithotomy under transumbilical endoscope and the application of absorbable clips in the surgical procedure are safe and feasible, and worthy of clinical promotion.]]> 2024/12/12 0:00:00 1, YIN Mengjie², HUANG Pengyu², HUANG Zongsheng², GUO Xianwen², LIANG Liexin², LIANG Yunxiao²]]> HUANG Yin¹, YIN Mengjie², HUANG Pengyu², HUANG Zongsheng², GUO Xianwen², LIANG Liexin², LIANG Yunxiao² 196true ［Abstract］　Objective　To explore the application value of laparoscopic debridement of pregnancy lesions in type Ⅱ unruptured corneal pregnancy. Methods　Twenty patients with type Ⅱ unruptured corneal pregnancy who were admitted to the Department of Gynecology of the People′s Hospital of Guangxi Zhuang Autonomous Region from October 2016 to October 2020 were selected. There were 15 cases with no fetal heart beat, 9 cases with pregnancy mass <3 cm, and 11 cases with pregnancy mass≥3 cm. All the patients were diagnosed by B-ultrasound and laparoscopy, and underwent laparoscopic debridement of pregnancy lesions. The amount of intraoperative blood loss, operation time, incidence of complications, changes of human chorionic gonadotropin(HCG) and hemoglobin(Hb) before and after operation, and postoperative pregnancy in the patients were analyzed. Results　The operation time of the 20 patients was (78.70±28.78)min, and their intraoperative blood loss was (54.00±39.80)mL. Preoperative fetal heart beat had no significant effect on intraoperative blood loss and operation time(P>0.05). The intraoperative blood loss in the preoperative pregnancy mass <3 cm group was less than that in the preoperative pregnancy mass ≥3 cm group, and the difference was statistically significant(P<0.05), but there was no significant difference in the operation time between the two groups(P>0.05). There were no complications such as uterine perforation and peripheral organ damage during the operation in all the 20 patients. The postoperative vaginal bleeding time lasted for (4.45±1.85)days. The time to resume diet after operation was (3.30±0.87)days, and the postoperative hospital stay was (4.00±0.65)days. The levels of HCG and Hb in the patients 2 days after operation were significantly lower than those before operation(P<0.05). Eleven patients had the intention of pregnancy, of which 6 cases(54.55%, 6/11) had normal pregnancy again and gave birth successfully. Conclusion　In type Ⅱ unruptured corneal pregnancy, laparoscopic debridement of pregnancy lesions protects the endometrium better, preserves the integrity of the myometrium, reduces the risk of reoperation, and has certain clinical application value.]]> 2024/12/12 8:08:13 YANG Yangfan, LIU Niping 195true ［Abstract］　Objective　To explore the clinical application value of “four taxonomies classification method” of pulmonary ground-glass opacity(GGO) lesions based on thin-section computed tomography(TSCT) scanning of the chest. Methods　The clinical data of 1 541 patients who underwent thoracoscopic surgery in Xuanwu Hospital Capital Medical University from January 2020 to December 2022 were retrospectively analyzed, including 2 005 pulmonary GGO lesions. The pulmonary GGO lesions were classified by using “four taxonomies classification method”: type Ⅰ, pure GGO(p-GGO) without vacuolar sign and(or) bronchiole sign; type Ⅱ, p-GGO with vacuolar sign and(or) bronchiole sign; type Ⅲ, heterogeneous GGO(h-GGO) of solid components visible only in the lung window; type Ⅳ, part-solid GGO(ps-GGO) of solid components visible in both the lung window and the mediastinal window. The relationship between the imaging characteristics of pulmonary GGO lesions［maximum diameter, type of lesions, occurrence site, classification and consolidation-to-tumor ratio(CTR)］ and the results of postoperative pathological examination was analyzed. Results　In the 1 541 patients, the time from the discovery of pulmonary GGO to receiving surgical treatment was 0.10-156.00 months, with an average of 11.23 months. During this period, there were no significant changes in 1 255 pulmonary GGO lesions(62.59%), and the maximum diameter increased in 536 pulmonary GGO lesions(26.73%), and the density increased in 45 pulmonary GGO lesions(2.24%), and the maximum diameter increased with increases in density in 169 pulmonary GGO lesions(8.43%). The maximum diameter of preoperative pulmonary GGO was (13.21±7.90)mm. The characteristics of the lesions were mainly single lesion, with a total of 1 098 lesions(54.76%), and the lesions were mostly distributed in the upper lobe of the right lung(757 lesions, 37.76%). Types of pulmonary GGO: 248 GGOs of type Ⅰ(12.37%), 682 GGOs of type Ⅱ(34.01%), 862 GGOs of type Ⅲ(42.99%) and 213 GGOs of type Ⅳ(10.62%), 950 GGOs with CTR≤25%(47.38%), 616 GGOs with 25%75%(6.23%). Among the pulmonary GGO lesions, there were 186 benign lesions(9.28%) and 1 819 malignant lesions(90.72%), including 744 glandular prodromal lesions and 1 075 infiltrative lesions. The analysis results showed that the results of postoperative pathological examination of the pulmonary GGO lesions were correlated with the maximum diameter of GGO, type of lesions, occurrence site, classification, CTR and the changes in GGO lesions during follow-up(P<0.05). The results of correspondence analysis showed that compared with the CTR of pulmonary GGO lesions, the scattered spots of pulmonary GGO lesions were more similar to the scattered spots of postoperative pathological malignant changes［atypical adenomatous hyperplasia(AAH), adenocarcinoma in situ(AIS), microinvasive adenocarcinoma(MIA) and invasive adenocarcinoma(IA)］ in the two-dimensional distribution map, suggesting that the imaging characteristics of pulmonary GGO lesions based on the “four taxonomies classification method” had good corresponding relations with postoperative pathological malignant changes. Conclusion　The “four taxonomies classification method” based on TSCT is helpful for clinicians to better evaluate the risk degree of early-stage lung cancer with GGO as the main imaging manifestation.]]> 2024/10/30 20:36:43 1, ZHANG Yi¹, GAO Yan², WANG Tengteng¹, LI Yuanbo¹, ZHANG Peilong¹, WANG Leiming³, WEI Xiuqin⁴]]> SU Lei¹, ZHANG Yi¹, GAO Yan², WANG Tengteng¹, LI Yuanbo¹, ZHANG Peilong¹, WANG Leiming³, WEI Xiuqin⁴ 194true ［Abstract］　Objective　To observe the effect of intravenous infusion of low-dose lidocaine in improving rebound pain after brachial plexus block. Methods　A total of 80 patients with unilateral upper limb fracture who underwent elective open reduction and internal fixation under brachial plexus block anesthesia were recruited in the 904th Hospital of Joint Logistics Support Force of Chinese People′s Liberation Army from July 2023 to November 2023. The patients were divided into the control group(after successful brachial plexus block, receiving intravenous infusion of normal saline) and the observation group(after successful brachial plexus block, receiving intravenous infusion of lidocaine at a dose of 100 mg) by using random number table method, with 40 cases in each group. In the control group, there was 1 case of incomplete block and 1 case was lost to follow up after operation. In the observation group, there was 1 case of incomplete block. After the patients with incomplete block and loss to follow up after operation were excluded, 38 cases were included in the control group and 39 cases in the observation group finally. The occurrence and duration of rebound pain, drug consumption of the analgesic pump, time of sensory block, rate of remedial analgesics and adverse reactions were compared between the two groups within 48 hours of nerve block. The Numerical Rating Scale(NRS) scores of resting state and activity state were compared between the two groups at different time points before and after surgery. Results　Compared with the control group, the observation group had lower incidence of rebound pain(17.95% vs 39.47%), less drug consumption of the analgesic pump［(105.62±11.21)mL vs (114.29±15.45)mL］, and the differences were statistically significant(P<0.05). There was no significant difference in the time of sensory block between the two groups(P>0.05). Among the patients with rebound pain, the duration of rebound pain in the observation group was shorter than that in the control group, and the difference was statistically significant［(2.43±1.27)h vs (3.87±1.19)h; t=2.589, P=0.018］. Compared with the control group, the observation group had lower NRS scores of resting state 12 hours and 24 hours after nerve block, and lower NRS scores of activity state 12 hours after nerve block, and the differences were statistically significant(P<0.05), and there were no statistically significant differences in NRS scores between the two groups at the other time points(P>0.05). The rate of remedial analgesics within 48 hours after operation in the observation group was significantly lower than that in the control group(P<0.05), and there were no significant differences in the incidence rate of nausea and vomiting and the incidence rate of dizziness between the two groups(P>0.05). Conclusion　Intravenous infusion of low-dose lidocaine can reduce the incidence rate and duration of rebound pain after brachial plexus block, reduce drug consumption of the analgesic pump and rate of remedial analgesics after operation, with fewer adverse reactions, which is conducive to rapid recovery of the patients.]]> 2024/10/30 20:36:43 1, ZHAO Yajie², ZHAI Ruirui², ZHOU Maitao³]]> LI Jiaying¹, ZHAO Yajie², ZHAI Ruirui², ZHOU Maitao³ 193true ［Abstract］　Objective　To observe the effect of transparent cap assisted endoscopic injection of polidocanol foam sclerosing agent at a high dose in treatment of grade Ⅱ-Ⅲ internal hemorrhoids. Methods　A total of 98 patients with grade Ⅱ-Ⅲ internal hemorrhoids who received the treatment of transparent cap assisted endoscopic injection of polidocanol foam sclerosing agent in Chang An Hospital from October 2022 to October 2023 were recruited and divided into conventional-dose group and high-dose group by using random number table method, with 49 cases in each group. The injection dose of polidocanol foam sclerosing agent in the conventional-dose group was 0.5-2.0 mL/injection site, and the injection dose of polidocanol foam sclerosing agent in the high-dose group was more than 2.0-4.0 mL/injection site. The therapeutic effect, treatment-related indicators, the occurrence of postoperative complications and the recurrence were compared between the two groups. The serum inflammatory indicators［tumor necrosis factor-alpha(TNF-α), C-reactive protein(CRP), interleukin-6(IL-6)］, anorectal dynamics indicators［rectal maximum tolerated volume(MTV), rectal resting pressure(RRP), anal maximum squeeze pressure(MSP)］, anal continence function score, anal sensory function score, and EuroQol five-dimensional questionnaire(EQ-5D) score were compared between the two groups before treatment, 1 month after treatment and 3 months after treatment. Results　The difference in therapeutic efficacy between the two groups was statistically significant(P<0.05), and the proportion of the patients cured in the high-dose group was higher than that in the conventional-dose group(79.59% vs 59.18%). The length of hospital stay in the high-dose group was significantly shorter than that in the conventional-dose group(P<0.05). After treatment, the levels of TNF-α, CRP, IL-6, MTV, MSP, anal continence function scores and anal sensory function scores in both groups showed a downward trend, while the levels of RRP and EQ-5D scores in both groups showed an upward trend, and the changes were greater in the high-dose group(P<0.05). At 1 month and 3 months after treatment, the levels of TNF-α, CRP, IL-6, MTV, MSP, anal continence function scores and anal sensory function scores in the high-dose group were lower than those in the conventional-dose group, while the RRP level and EQ-5D scores in the high-dose group were higher than those in the conventional-dose group, and the differences were statistically significant(P<0.05). There was no significant difference in the total incidence of postoperative complications between the two groups(10.20% vs 6.12%; χ²=0.544, P=0.461). The follow-up was conducted for 6 months after treatment, and there were no cases of recurrence in the high-dose group, while there were 6 cases of recurrence in the conventional-dose group, and the recurrence rate in the high-dose group was significantly lower than that in the conventional-dose group(0.00% vs 12.24%; χ²=6.391, P=0.011). Conclusion　Transparent cap assisted endoscopic injection of polidocanol foam sclerosing agent at a high dose can improve the cure rate in treatment of grade Ⅱ-Ⅲ internal hemorrhoids, inhibit the inflammatory response, promote the recovery of anorectal dynamics, improve the anal function, improve the quality of life, and reduce the recurrence rate, and is worthy of clinical recommendation.]]> 2024/10/30 20:36:43 SHI Jie, LI Wenzhong, ZHOU Yi, ZHOU Qingwen 192true ［Abstract］　Objective　To explore the application effect of magnetic resonance imaging(MRI) tracking iron metabolism in rat brain based on transferrin receptor(TfR), and to provide reference for clinical development of methods to monitor the therapeutic effect of Alzheimer′s disease. Methods　Twelve healthy adult Sprague-Dawley(SD) rats were selected and randomly divided into experimental group and control group, with 6 rats in each group. In the rats of the experimental group, TfR lentiviruses were injected into the striatum of the left cerebral hemisphere(TfR transfection group), and empty vector EGFP lentiviruses were injected into the striatum of the right cerebral hemisphere(EGFP transfection group). In the rats of the control group, the same volume of normal saline was injected into both sides of the striatum of the cerebral hemisphere(normal group). Two weeks later, transferrin(Tf)-conjugate of ultra-small super-paramagnetic iron oxide nanoparticles(USPIO)(Tf-USPIO) tracer was injected into the femoral vein of the rats at dose of 15 mg/kg, and MRI examination was performed for the rats before the injection and 1 day after the injection. After the MRI examination was completed, the rats were anesthetized with sodium pentobarbital and were sacrificed by using cervical dislocation method, and the brain striatum tissues of the rats were collected for immunofluorescence staining and Prussian blue staining. Real-time quantitative polymerase chain reaction(PCR) was used to detect the expression of TfR mRNA of the brain striatum tissues in rats. Results　The results of real-time quantitative PCR showed that the expression level of TfR mRNA of the brain tissues in the TfR transfection group was significantly higher than that in the EGFP transfection group and the normal group(P<0.05). The results of immunofluorescence staining and Prussian blue staining showed that TfR lentiviruses were successfully transfected into the brain tissues of the rats and the iron accumulation in the brain of Alzheimer′s disease was successfully simulated. Before and after the injection of Tf-USPIO tracer, there were no significant changes in the MRI examination results of the rats in the control group, and obvious signal changes were observed in the left striatum of the rats in the experimental group and no obvious signal changes were observed in the right striatum of the rats in the experimental group. Conclusion　Tf-USPIO tracer can specifically bind to TfR in brain tissues and can be displayed through MRI, providing a reference for the clinical development of methods to monitor the therapeutic effect of Alzheimer′s disease.]]> 2024/10/30 0:00:00 1, LI Ning¹, SHANG Yuchun¹, HU Beiquan²]]> ZHANG Yesen¹, LI Ning¹, SHANG Yuchun¹, HU Beiquan² 191true ［Abstract］　Objective　To explore the relationship of serum CBL proto-oncogene like 1(CBLL1) and matrix metalloproteinase 3(MMP3) levels with hemorrhagic transformation(HT) and prognosis after intravenous thrombolysis in acute ischemic stroke(AIS) patients. Methods　A total of 264 AIS patients who received intravenous thrombolytic therapy in Third Hospital of Heilongjiang Province from January 2020 to December 2022 were recruited. According to the occurrence of HT after intravenous thrombolysis, the patients were divided into HT group(42 cases) and non-HT group(222 cases). The clinical data, the levels of serum CBLL1 and MMP3 before treatment and the prognosis 3 months after treatment were compared between the two groups. Multivariate logistic regression was used to analyze the influencing factors of HT occurrence after intravenous thrombolysis in the AIS patients. Receiver operating characteristic(ROC) curve was used to analyze the efficacy of serum CBLL1 and MMP3 levels in predicting the occurrence and prognosis of HT after intravenous thrombolysis in the AIS patients. Results　Compared with those in the non-HT group, the National Institutes of Health Stroke Scale(NIHSS) scores, serum CBLL1 and MMP3 levels, and the incidence of adverse prognosis［modified Rankin Scale(mRS)≥3 points］ in the HT group were high, and the differences were statistically significant(P<0.05). The results of multivariate logistic regression analysis showed that relatively high NIHSS scores［OR(95%CI)=1.298(1.028-1.639)］ and serum CBLL1［OR(95%CI)=1.320(1.023-1.704］ and MMP3［OR(95%CI)=1.406(1.103-1.793］ levels were independent risk factors for occurrence of HT after intravenous thrombolysis in the AIS patients(P<0.05). The results of ROC curve analysis showed that the combined detection of serum CBLL1 and MMP3 levels could effectively predict the occurrence of HT after intravenous thrombolysis［AUC(95%CI)=0.936(0.892-0.965), P<0.001］ and the prognosis［AUC(95%CI)=0.941(0.901-0.985), P<0.001］ in the AIS patients. Conclusion　The elevated serum CBLL1 and MMP3 levels in AIS patients are associated with the occurrence of HT and poor prognosis after intravenous thrombolysis, which helps clinicians identify the high-risk patients and give early interventions.]]> 2024/10/30 20:36:43 1, JIN Hongwen², SHE Bixia²]]> MU Xiaochun¹, JIN Hongwen², SHE Bixia² 190true ［Abstract］　Objective　To observe the cardiopulmonary protective effect of dexmedetomidine combined with opioids on patients undergoing cardiopulmonary bypass surgery during the perioperative period in order to provide clinical guidance for rational application of anesthetic drugs. Methods　One hundred and twenty patients who underwent single valve replacement under general anesthesia and hypothermia cardiopulmonary bypass in the People′s Hospital of Guangxi Zhuang Autonomous Region from May 2016 to March 2018 were recruited and divided into sufentanil group(S group), fentanyl group(F group), sufentanil+dexmedetomidine group(SD group) and fentanyl+dexmedetomidine group(FD group) by random number table method, with 30 cases in each group. The patients′ intraoperative conditions were compared among the four group. The inflammatory factors, creatine kinase isoenzyme-MB(CK-MB) and cardiac troponin I(cTnI) levels were detected 1 day before entering the operation room(T₀), 1 day after operation(T₁), 3 days after operation(T₂), and 7 days after operation(T₃). Oxygenation index(OI), alveolar arterial pressure difference［P(A-aDO₂)］ and the other indexes of lung function were calculated. Results　The dosage of sufentanil in the SD group was significantly lower than that in the S group(P<0.05) and the dosage of fentanyl in the FD group was significantly lower than that in the F group(P<0.05). The dosages of propofol and midazolam in the SD group were lower than those in the other three groups, and the differences were statistically significant(P<0.05). At T₁ to T₃ time points, the levels of white blood cell(WBC), percentage of neutrophils(N%), C-reactive protein(CRP) and hypersensitive C-reactive protein(hs-CRP) in the SD group and the FD group were significantly lower than those in the S group and the F group, respectively(P<0.05). At T₁ time point, the levels of CRP and hs-CRP in the SD group were significantly lower than those in the FD group(P<0.05). The levels of WBC, N%, CRP and hs-CRP in the SD group were significantly lower than those in the FD group at T₂ and T₃ time points(P<0.05). At T₁ to T₃ time points, the levels of CK-MB and cTnI in the SD group and the FD group were significantly lower than those in the S group and the F group, respectively(P<0.05). At T₁ time point, the level of cTnI in the SD group was significantly lower than that in the FD group(P<0.05). At T₂ time point, the levels of CK-MB and cTnI in the SD group were significantly lower than those in the FD group(P<0.05). At T₃ time point, the level of CK-MB in the SD group was significantly lower than that in the FD group(P<0.05). Conclusion　Dexmedetomidine combined with sufentanil or fentanyl regimen can reduce inflammatory stress response and cardiopulmonary organ function injury, and dexmedetomidine combined with sufentanil regimen is more effective.]]> 2024/10/30 20:36:43 1,2, SONG Siming¹, LU Liuyu¹, JIANG Yuhua¹, LI Jun¹, MA Li¹]]> GONG Zheng^1,2, SONG Siming¹, LU Liuyu¹, JIANG Yuhua¹, LI Jun¹, MA Li¹ 189true ［Abstract］　Objective　To compare the effects of iterative model reconstruction(IMR) algorithm and hybrid iterative reconstruction(iDose⁴) algorithm on the image quality of low-dose left atrial pulmonary vein CT. Methods　A total of 30 patients undergoing radiofrequency ablation due to atrial fibrillation in the Second Affiliated Hospital of Guangxi Medical University from January 2020 to December 2020 were recruited. The recruited patients received low-dose left atrial pulmonary vein CT examination with tube voltage of 80 kV and tube current of 350 mA. After scanning, the images were reconstructed by using IMR algorithm(IMR group) and iDose⁴ algorithm(iDose⁴ group), and the image quality in the two groups was subjectively scored. The region of interest(ROI) was delineated at the opening of the right superior pulmonary vein(RSPV), the right inferior pulmonary vein(RIPV), the left superior pulmonary vein(LSPV) and the left inferior pulmonary vein(LIPV), and the ROI was delineated for the left atrium, descending aorta and chest wall fat at the central level of the left atrium. The areas with uniform density in the ROI were selected and delineated, and the CT value and noise value［standard deviation(SD) value］ in each ROI were recorded. The contrast-to-noise ratio(CNR) and signal-to-noise ratio(SNR) of the left atrium, RSPV, RIPV, LSPV and LIPV were calculated, and the objective scores were compared between the two groups. Results　The subjective evaluations of the images in the IMR group and the iDose⁴ group by two radiologists were highly consistent, with intra-class correlation coefficient(ICC) being 0.895(P<0.001) and 0.920(P<0.001), respectively. The IMR group had significantly higher subjective scores of image quality than the iDose⁴ group［(4.50±0.57)points vs (3.86±0.68)points; t=3.898, P<0.001］. The image noise values(SD values) of the left atrium, RSPV, RIPV, LSPV and LIPV in the IMR group were lower than those in the iDose⁴ group, and the SNR and CNR in the IMR group were higher than those in the iDose⁴ group, and the differences were statistically significant(P<0.05). The CT values of the left atrium, RSPV, RIPV, LSPV and LIPV in the IMR group were lower than those in the iDose⁴ group, and the differences were statistically significant(P<0.05). Conclusion　Compared with iDose⁴ algorithm, IMR algorithm can significantly reduce noise, improve SNR and CNR in low-dose left atrial pulmonary vein CT images, thereby improving the image quality. IMR algorithm is worth promoting in clinical application.]]> 2024/10/30 20:36:43 DU Guoxiang, LIANG Zhimei, LI Min, LUO Yingyi, LU Bingfeng, LI Weixiong 188true ［Abstract］　Objective　To explore the influence of early blood glucose indicators on survival prognosis of sepsis patients in intensive care unit(ICU). Methods　The clinical data of 251 patients with sepsis who were admitted to the ICU of Fourth People′s Hospital of Langfang from January 2018 to January 2024 were retrospectively analyzed. According to the patients′ survival prognosis, they were divided into survival group(n=165) and death group(n=86). The general clinical data, treatment-related indicators and blood glucose indicators were compared between the two groups. The influence of blood glucose indicators on survival prognosis of the patients was analyzed by using multivariate logistic regression. Results　Compared with the survival group, the death group had older age, higher Acute Physiology and Chronic Health Evaluation(APACHE) Ⅱ scores, higher Sequential Organ Failure Assessment(SOFA) scores, more failing organs, higher proportion of patients receiving drugs for raising blood pressure and proportion of patients receiving renal replacement therapy, longer mechanical ventilation time, and greater coefficient of variation(CV) of blood glucose values and stress hyperglycemia ratio(SHR), and the differences were statistically significant(P<0.05). After adjusting for general clinical data, disease severity and treatment-related indicators, the results of multivariate logistic analysis showed that higher SHR level was a risk factor of death for sepsis patients in ICU(P<0.05), while CV of blood glucose values had no significant effect on prognosis of sepsis patients in ICU(P>0.05). Conclusion　Higher SHR level is a risk factor of death for sepsis patients in ICU, which is worthy of clinicians′ attention.]]> 2024/10/30 20:36:43 1, LI Yunwei²]]> ZUO Yanyan¹, LI Yunwei² 187true ［Abstract］　Objective　To explore the mechanism of Ban′s Culuan Zhuyun Decoction(CLZYD) in treatment of polycystic ovarian syndrome(PCOS) based on network pharmacology and molecular docking technology. Methods　The active pharmaceutical compounds and effect targets of CLZYD were searched in TCMSP and BATMANTCM databases. The disease targets of PCOS were searched in GeneCards, DisGeNET, UniProt and Drugbank databases, and the drug-disease common target interaction network was constructed. The Cytoscape software was used to construct a drug-compound-target-disease network, and a protein-protein interaction(PPI) network was constructed by using STRING platform. The Gene Ontology(GO) function enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis were performed by using the Metascape gene function analysis platform, and the core effect targets and the core active pharmaceutical compounds were verified by using molecular docking. Results　A total of 184 active pharmaceutical compounds, 362 targets of active drug compounds, 1 807 disease targets and 165 drug-disease common targets were obtained. The results of drug-compound-target-disease network analysis showed that the core active pharmaceutical compounds were quercetin, kaempferol and β-sitosterol. The results of PPI network analysis showed that the core effect targets included RAC-α serine/threonine-protein kinase(AKT1), tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), interleukin-1β(IL-1β) and tumor protein 53(TP53). The results of GO function enrichment analysis showed that the biological processes of CLZYD for the treatment of PCOS mainly involved response to hormone, membrane raft and transcription factor binding. The results of KEGG pathway enrichment analysis showed that the mechanism of CLZYD for the treatment of PCOS might be related to pathways of cancer, AGE/RAGE signaling pathway in diabetic complications and chemical carcinogensis-receptor activation. The results of molecular docking showed that quercetin, kaempferol and β-sitosterol had good binding activity with AKT1, TNF-α, IL-6, IL-1β and TP53. Conclusion　CLZYD can exert therapeutic effects on PCOS through multiple compounds, targets and pathways.]]> 2024/10/30 0:00:00 1, WANG Ruize², WU Liming¹, LIU Yunjia¹, ZHOU Huanglei¹, LI Mingxing¹, HUANG Yulin¹, WU Yuanyuan³, BAI Lin¹, WAN Chenyanghao⁴, LUO Yang¹, HE Guozhen¹]]> YUE Xiaolei¹, WANG Ruize², WU Liming¹, LIU Yunjia¹, ZHOU Huanglei¹, LI Mingxing¹, HUANG Yulin¹, WU Yuanyuan³, BAI Lin¹, WAN Chenyanghao⁴, LUO Yang¹, HE Guozhen¹ 186true ［Abstract］　Objective　To explore the clinical efficacy of Shenqi Qiangjing Decoction in treating oligoasthenozoospermia patients and its effect on in vitro fertilization(IVF) outcomes. Methods　A total of 209 patients with oligoasthenozoospermia who were admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region and the First Affiliated Hospital of Guangxi University of Chinese Medicine from October 2020 to December 2022 were recruited. According to the patients′ treatment intentions, they were divided into observation group(receiving Shenqi Qiangjing Decoction treatment, 108 cases) and control group(receiving L-carnitine treatment, 101 cases). After 3 months of treatment, IVF-assisted pregnancy was performed on all the patients. The semen quality and IVF outcomes were compared between the two groups. Results　After treatment, sperm DNA fragmentation index(DFI) was significantly decreased in both groups(P<0.05), and the sperm DFI in the observation group was lower than that in the control group, and the difference was statistically significant(P<0.05). There was no statistical significance in sperm acrosome integrity rate in the control group before and after treatment(P>0.05), and sperm acrosome integrity rate in the observation group after treatment was significantly higher than that before treatment(P<0.05), and the observation group had higher sperm acrosome integrity rate than the control group, and the differences were statistically significant(P<0.05). There were no significant differences in the sperm progressive motility rate, sperm concentration, total sperm count and normal sperm morphology rate between the two groups after treatment(P>0.05). The fertilization rate and excellent embryo rate in the observation group were significantly higher than those in the control group(P<0.05), and there were no significant differences in cleavage rate and clinical pregnancy rate between the two groups(P>0.05). Conclusion　Shenqi Qiangjing Decoction can improve the semen quality of the patients with oligoasthenozoospermia and optimize the IVF outcomes, and improve some indicators more effectively than L-carnitine.]]> 2024/10/30 20:36:43 1, LI Dawen², BIN Bin³, LU Haiwang³, PANG Taisen²]]> CHEN Qigui¹, LI Dawen², BIN Bin³, LU Haiwang³, PANG Taisen² 185true ［Abstract］　Objective　To evaluate the value of ferroptosis-related alternative splicing events in predicting the prognosis of gastric cancer and to explore their impacts on the tumor microenvironment and response to the treatment. Methods　Based on the transcriptome data of gastric cancer from The Cancer Genome Atlas(TCGA) database, ferroptosis-related alternative splicing events were screened. A nomogram model for predicting the prognosis of gastric cancer was constructed using LASSO regression and multivariate Cox regression. The predictive ability of the model and its underlying mechanisms were verified by combining functional enrichment analysis, assessment of immune cell infiltration and in vitro experiments. Results　It was finally determined that a nomogram model for predicting the prognosis of gastric cancer was constructed based on 12 alternative splicing events related to ferroptosis. According to the median risk scores, the patients were divided into high-risk group(203 patients) and low-risk group(203 patients). The low-risk group exhibited the characteristics of immune activation, with a significantly improved prognosis(P<0.05), and was more likely to benefit from targeted therapy and immunotherapy. The in vitro experiments confirmed that the high expression of the splicing factor SF3A2 promoted cell proliferation by inhibiting ferroptosis. Conclusion　Ferroptosis-related alternative splicing events have good efficiency in predicting the prognosis and treatment response of gastric cancer, providing a potential basis for formulating individualized treatment strategies.]]> 2025/9/30 10:04:06 *, GUO Lei, FAN Xiaopeng, GUO Peng]]> LI Zhiyong, LI Yuxi^*, GUO Lei, FAN Xiaopeng, GUO Peng 184true ［Abstract］　Objective　To analyze the expression levels of factors related to T-cell receptor(TCR) signaling pathway in peripheral blood mononuclear cells(PBMCs) of patients with sepsis-related acute respiratory distress syndrome(ARDS). Methods　Twenty-two patients with sepsis-related ARDS who were treated in the Second Affiliated Hospital of Guangxi Medical University from March 2022 to November 2022 were recruited as case group, and 21 healthy volunteers were recruited as control group during the same period. A total of 8 mL of peripheral venous blood was collected from each research subjects, and PBMCs were isolated, and the mRNA expression levels of TCR signaling pathway-related factor CD247, linker for activation of T cells(LAT) and interleukin-2-inducible T-cell kinase(ITK) were detected by using real-time fluorescent quantitative polymerase chain reaction(RT-qPCR). The expression levels of serum interleukin-6(IL-6), interleukin-17A(IL-17A) and interleukin-10(IL-10) were detected by using enzyme-linked immunosorbent assay(ELISA). Broncho-alveolar lavage fluid(BALF) was collected from the case group for Swiss-Giemsa staining to observe the inflammatory cells. Results　Compared with those in the control group, the expression levels of CD247, LAT and ITK mRNA in PBMCs in the case group were significantly decreased(P<0.05), and the levels of serum IL-6, IL-10 and IL-17A in the case group were significantly increased(P<0.05). In the BALF of the patients with sepsis-related ARDS, significant increases in neutrophils, lymphocytes and red blood cells could be observed, and the volume of macrophages increased, and necrotic and desquamated alveolar epithelial cells were observed. Conclusion　Expressions of TCR signaling pathway-related factors in PBMCs of patients with sepsis-related ARDS are significantly decreased, which might be one of the mechanisms of sepsis progression.]]> 2025/9/30 10:04:06 1, YANG Hui¹, YANG Yanli², PENG Liang¹, FENG Jihua³, ZHANG Jianfeng⁴]]> ZENG Xiaoliang¹, YANG Hui¹, YANG Yanli², PENG Liang¹, FENG Jihua³, ZHANG Jianfeng⁴ 183true ［Abstract］　Objective　To observe the clinical effects of endotracheal surface anesthesia with tetracaine under bronchoscopic direct vision combined with general anesthesia using a laryngeal mask in bronchoscopy. Methods　A total of 160 patients who underwent elective bronchoscopy in NORINCO General Hospital from June 2024 to December 2024 were recruited and randomly divided into observation group(undergoing endotracheal surface anesthesia with tetracaine under bronchoscopic direct vision combined with general anesthesia using a laryngeal mask) and control group(undergoing endotracheal surface anesthesia with lidocaine under bronchoscopic direct vision combined with general anesthesia using a laryngeal mask) by using random number table method, with 80 patients in each group. The levels of heart rate(HR), mean arterial pressure(MAP) and pulse oxygen saturation(SpO₂) were compared between the two groups before anesthesia(T₀), 1 minute after the start of the examination(T₁), 5 minutes after the start of the examination(T₂) and 2 minutes after the removal of the laryngeal mask(T₃). The choking cough scores during examination, cough scores during anaesthetic awakening period, incidence of itching in the airway, and the dosages of etomidate and propofol mixed liquids and remifentanil were compared between the two groups. The occurrence of adverse reactions in the patients during anaesthetic awakening period was recorded. Results　At the time points from T₀ to T₃, there were no significant changes in the levels of SpO₂, MAP and HR in the observation group(P>0.05), and the levels of MAP and HR in the control group showed a trend of first increasing and then decreasing(P<0.05), while the level of SpO₂ showed no significant changes(P>0.05). At the time points of T₁ and T₂, the levels of MAP and HR in the observation group were lower than those in the control group, with statistically significant differences(P<0.05). The dosages of etomidate and propofol mixed liquids and remifentanil during the examination in the observation group were significantly less than those in the control group(P<0.05). Compared with the control group, the observation group showed better choking cough condition during examination and better coughing condition during anaesthetic awakening period, and had a lower incidence of itching in the airway, with statistically significant differences between the two groups(P<0.05). The incidence rates of hypertension and arrhythmia in the observation group were significantly lower than those in the control group(11.25% vs 26.25%, χ²=5.932, P=0.015; 10.00% vs 31.25%, χ²=11.063, P=0.001). No hypoxemia or bronchospasm occurred in either group. Conclusion　Endotracheal surface anesthesia with tetracaine under bronchoscopic direct vision combined with general anesthesia using a laryngeal mask can effectively inhibit cardiovascular stress responses and airway responses in bronchoscopy, reduce the incidence of adverse reactions during anaesthetic awakening period, and has clinical promotion and application value.]]> 2025/9/30 10:04:06 1, SHU Tiankun¹, NIE Huiyong², WANG Shuang¹, QUAN Pei¹, HUANG Jing¹]]> ZHOU Ying¹, SHU Tiankun¹, NIE Huiyong², WANG Shuang¹, QUAN Pei¹, HUANG Jing¹ 182true ［Abstract］　Objective　To explore the relation of serum semaphorin 3A(SEMA3A) and semaphorin 4D(SEMA4D) with risk stratification and prognosis in pediatric patients with acute lymphoblastic leukemia(ALL). Methods　A total of 128 pediatric patients with ALL who were admitted to General Hospital of Ningxia Medical University from March 2019 to March 2023 were recruited. According to the risk stratification of ALL in these pediatric patients, they were divided into low-risk group(31 patients), medium-risk group(62 patients) and high-risk group(35 patients). The levels of serum SEMA3A and SEMA4D were measured using enzyme-linked immunosorbent assay(ELISA). According to the prognosis of the pediatric patients at 12 months after treatment, they were divided into poor prognosis group(26 patients) and good prognosis group(102 patients). The clinical data were compared between the two groups. Spearman rank correlation was used to analyze the correlation of the levels of SEMA3A and SEMA4D with the risk stratification of the ALL pediatric patients. Multivariate logistic regression was used to analyze the influencing factors of poor prognosis in the pediatric patients with ALL. Results　The serum SEMA3A levels in the low-risk group, the medium-risk group and the high-risk group decreased successively, while their serum SEMA4D levels increased successively, with statistically significant differences(P<0.05). The results of Spearman rank correlation analysis showed that the SEMA3A level was negatively correlated with the risk stratification of ALL in the pediatric patients(r_s=-0.689, P<0.001), while the SEMA4D level was positively correlated with the risk stratification of ALL in the pediatric patients(r_s=0.452, P<0.001). Compared with the good prognosis group, the poor prognosis group was older, and had higher levels of white blood cell count(WBC) and SEMA4D, and lower levels of SEMA3A, with statistically significant differences(P<0.05). There were statistically differences in immunoclassification and risk stratification between the two gropus(P<0.05). The results of multivariate logistic regression analysis showed that older age, higher WBC level, higher SEMA4D level, immunoclassification of T-ALL and high-risk types in risk stratification were independent risk factors for poor prognosis in the pediatric patients with ALL(P<0.05), while higher SEMA3A level was an independent protective factor for poor prognosis in the pediatric patients with ALL(P<0.05). Conclusion　The levels of serum SEMA3A and SEMA4D in pediatric patients with ALL are closely related to risk stratification and prognosis. A decrease in SEMA3A levels and an increase in SEMA4D levels can raise the risk of poor prognosis.]]> 2025/9/30 10:04:06 TANG Changchao, ZUO Feng, BAI Hua, WEI Xueli 181true ［Abstract］　Objective　To explore the expressions of kinesin family member 18B(KIF18B) and anti-silencing function 1B(ASF1B) in endometrial carcinoma(EC) tissues, and to analyze the association of the expressions of KIF18B and ASF1B in endometrial carcinoma tissues with clinicopathological characteristics and survival prognosis. Methods　The expression levels of KIF18B, ASF1B mRNA and protein in normal tissues and uterine corpus endometrial carcinoma(UCEC) tissues were analyzed by using bioinformatics methods. The clinical data of 226 patients with EC who were admitted to Xi′an People′s Hospital(Xi′an Fourth Hospital) from July 2016 to July 2021 were collected. During the operation, the EC tissues and their adjacent tissues(3 to 5 cm away from the edge of the EC tissues) of the EC patients were collected. The expression levels of KIF18B mRNA and ASF1B mRNA in the EC tissues and their adjacent tissues were detected by using real-time fluorescent quantitative polymerase chain reaction(FQ-PCR). The expressions of KIF18B and ASF1B proteins in the EC tissues and their adjacent tissues were detected by using immunohistochemical staining(SP method). After the operation, the patients were followed up for 3 years and their survival prognosis was recorded. Results　The Cancer Genome Atlas(TCGA) database showed that the levels of KIF18B mRNA and ASF1B mRNA in the UCEC tissues were significantly higher than those in the normal tissues(P<0.05). Clinical Proteomic Tumor Analysis Consortium(CPTAC) database showed that the expression level of ASF1B protein in the UCEC tissues was significantly higher than that in the normal tissues(P<0.05). The levels of KIF18B mRNA and ASF1B mRNA in the EC tissues were significantly higher than those in their adjacent tissues(P<0.05). The positive expression rates of KIF18B protein and ASF1B protein in the EC tissues were significantly higher than those in their adjacent tissues(P<0.05). The expressions of KIF18B and ASF1B proteins were associated with the depth of myometrial infiltration, International Federation of Gynecology and Obstetrics(FIGO) stage and lymph node metastasis(P<0.05). The results of multivariate Cox regression analysis showed that FIGO stage Ⅲ, positive expression of KIF18B protein, and positive expression of ASF1B protein were independent risk factors for death in the EC patients(P<0.05). The survival prognosis of the patients with negative expressions of KIF18B and ASF1B proteins was significantly better than that of the patients with positive expressions of KIF18B and ASF1B proteins(log-rank test: χ²=8.518, P=0.004; log-rank test: χ²=9.993, P=0.002). Conclusion　In EC tissues, both KIF18B and ASF1B are highly expressed, and both are closely associated with the depth of myometrial infiltration, FIGO stage, lymph node metastasis and survival prognosis.]]> 2025/9/30 10:04:06 YE Xiaolin, LIANG Xinli, ZHANG Chao 180true ［Abstract］　Objective　To explore the levels of serum microRNA-128-3p(miR-128-3p) and microRNA-211-5p(miR-211-5p) in epilepsy patients and to analyze their correlations with the the degree of electroencephalogram(EEG) abnormalities. Methods　A total of 120 epilepsy patients admitted to Qingdao Chengyang People′s Hospital from March 2022 to June 2023 were recruited as epilepsy group, and other 120 healthy individuals who underwent physical examination during the same period were selected as control group. According to the EEG examination results of these epilepsy patients, they were further divided into mild abnormality group(41 patients), moderate abnormality group(46 patients), and severe abnormality group(33 patients). The levels of serum miR-128-3p and miR-211-5p were determined by using quantitative real-time fluorescence polymerase chain reaction(qRT-PCR). The correlations of serum miR-128-3p and miR-211-5p levels with the degree of EEG abnormalities were analyzed by using Spearman rank correlation. The receiver operating characteristic(ROC) curve was used to analyze the efficacy of serum miR-128-3p and miR-211-5p levels in diagnosing severe EEG abnormality. Results　The levels of serum miR-128-3p and miR-211-5p in the epilepsy group were significantly lower than those in the control group(P<0.05). The levels of serum miR-128-3p and miR-211-5p in the epilepsy patients of the severe abnormality group and the moderate abnormality group were significantly lower than those in the epilepsy patients of the mild abnormality group(P<0.05), and the levels of miR-128-3p and miR-211-5p in the severe abnormality group were lower than those in the moderate abnormality group, with statistically significant differences(P<0.05). The results of Spearman rank correlation analysis showed that the levels of serum miR-128-3p and miR-211-5p were negatively correlated with the degree of EEG abnormalities(r_s=-0.484, P<0.001; r_s=-0.579, P<0.001). The results of ROC curve analysis showed that the levels of serum miR-128-3p and miR-211-5p could effectively diagnose severe EEG abnormality(P<0.05), and the diagnostic efficacy of the combination of the two indicators was higher than that of a single indicator(Z_{combination of the two indicators-miR-128-3p}=2.850, P=0.004; Z_{combination of the two indicators-miR-211-5p}=2.562, P=0.010), with a sensitivity of 81.82% and a specificity of 79.31%. Conclusion　The levels of miR-128-3p and miR-211-5p in the serum of epilepsy patients are decreased, which are negatively correlated with the degree of EEG abnormalities. The combination of the two indicators can better diagnose severe EEG abnormality in the patients.]]> 2025/9/30 10:04:06 1, KONG Hui², WANG Rui³]]> YU Haiyan¹, KONG Hui², WANG Rui³ 179true ［Abstract］　Objective　To observe the clinical efficacy of transumbilical single-incision plus one-port laparoscopy with VICRYL baseball suture technique in treating giant uterine fibroids with a diameter ≥10 cm. Methods　A retrospective analysis was conducted on the clinical data of the patients with giant uterine fibroids who were admitted to the People′s Hospital of Langfang City from September 2021 to June 2023. In the patients, 52 patients who received the treatment of transumbilical single-incision plus one-port laparoscopy with VICRYL baseball suture technique were selected as observation group, while other 52 patients who received conventional four-port laparoscopic surgery by using a matching method at a ratio of 1∶1 were selected as control group. The perioperative indicators, postoperative Visual Analogue Scale(VAS) scores, trauma stress indicators［superoxide dismutase(SOD), reactive oxygen species(ROS), C-reactive protein(CRP), glutathione peroxidase(GSH-Px)］, patient subjective satisfaction with surgical incision and the occurrence of complications were compared between the two groups. Results　The duration of operation in the observation group was shorter than that in the control group, and time to first anal exhaust and time to first ambulation after operation in the observation group was earlier than that in the control group, with statistically significant differences(P<0.05). There were no statistically significant differences in intraoperative blood loss and hospital stay between the two groups(P>0.05). The VAS scores at 6 hours, 9 hours, 12 hours, 24 hours and 32 hours after operation in the observation group were significantly lower than those in the control group(P<0.05). On the first and third days after operation, the levels of CRP and ROS in the observation group were lower than those in the control group, while the levels of GSH-Px and SOD in the observation group were higher than those in the control group, with statistically significant differences(P<0.05). The rate of patient subjective satisfaction with surgical incision in the observation group was significantly higher than that in the control group(98.08% vs 75.00%; χ²=11.886, P=0.001). There was no statistically significant difference in the incidence of complications between the two groups(3.85% vs 1.92%; χ²=0.000, P=1.000). Conclusion　The transumbilical single-incision plus one-port laparoscopy with VICRYL baseball suture technique is a safe and feasible treatment for giant uterine fibroids with a diameter ≥10 cm, with good patient subjective satisfaction with surgical incision, and benefits for early postoperative recovery of the patients.]]> 2025/9/30 10:04:06 ZHAO Yanhong, GAO Jianhong, XU Donglei, SUN Peipei, ZHANG Jing 178true ［Abstract］　Objective　To compare the effects of flexible negative pressure aspiration sheath and intelligent pressure-controlled aspiration sheath respectively combined with ureteroscopic laser lithotripsy and stone extraction in treatment of upper urinary tract stones smaller than 2 cm. Methods　A total of 160 patients with upper urinary tract stones smaller than 2 cm who were admitted to the First People′s Hospital of Yulin from June 2023 to June 2024 were recruited and divided into group A(receiving flexible negative pressure aspiration sheath combined with ureteroscopic laser lithotripsy and stone extraction) and group B(receiving intelligent pressure-controlled aspiration sheath combined with ureteroscopic laser lithotripsy and stone extraction) by using random number table method, with 80 patients in each group. The operation time, stone clearance rate, length of hospital stay and the levels of postoperative white blood cell count(WBC), C-reactive protein(CRP) and procalcitonin(PCT) were compared between the two groups. The occurrence of complications was recorded in the two groups. Results　The operation time in group B was shorter than that in group A, and the postoperative WBC and CRP levels in group B were lower than those in group A, and the differences were statistically significant(P<0.05). There were no statistically significant differences in the length of hospital stay and postoperative PCT level between the two groups(P>0.05). The stone clearance rate in group A was significantly higher than that in group B at 1 month after the operation(83.75% vs 70.00%; χ²=4.254, P=0.039), but there was no statistically significant difference in the stone clearance rate between the two groups at 3 months after the operation(93.75% vs 88.75%; χ²=1.252, P=0.263). There was no statistically significant difference in the incidence of postoperative complications between the two groups(P>0.05). Conclusion　The applications of flexible negative pressure aspiration sheath and intelligent pressure-controlled aspiration sheath in ureteroscopic laser lithotripsy and stone extraction are safe and effective in treatment of upper urinary tract stones smaller than 2 cm. Intelligent pressure-controlled aspiration sheath combined with ureteroscopic laser lithotripsy and stone extraction is superior to flexible negative pressure aspiration sheath combined with ureteroscopic laser lithotripsy and stone extraction in shortening the operation time and controlling postoperative inflammation.]]> 2025/9/30 10:04:06 LIU Mingqi, LIANG Bin, ZHANG Zhenqiang, DANG Bowen, LIU Xingze, YE Jinhan, HUANG Beiyuan, CHEN Guan, TAN Baofei, PANG Guijian, XU Wei, LIU Chengbei 177true ［Abstract］　Objective　To compare and analyze the effects of single-photon emission computed tomography-computed tomography(SPECT-CT) and conventional computed tomography(CT)-guided implantation of ¹²⁵I seeds on salivary gland function and survival prognosis in patients with radioiodine-refractory differentiated thyroid cancer(RAIR-DTC). Methods　A total of 116 patients with RAIR-DTC who were admitted to Changde Hospital, Xiangya School of Medicine, Central South University(the First People′s Hospital of Changde City) from February 2016 to February 2019 were selected and divided into observation group and control group according to random envelope method, with 58 patients in each group. The control group underwent conventional CT-guided implantation of ¹²⁵I seeds, while the observation group underwent SPECT-CT-guided implantation of ¹²⁵I seeds. The clinical efficacy, salivary gland function, European Quality of Life Five-Dimension Five-Level Scale(EQ-5D-5L) scores, incidence of adverse reactions and survival prognosis within 5 years after treatment were compared between the two groups. Results　The disease control rate of the observation group was significantly higher than that of the control group(P<0.05). At 3 months(T₁) and 6 months(T₂) after treatment, the maximum concentration rate(MAR) of the parotid gland, the maximum secretion rate(MSR) of the parotid gland after acid stimulation, the MAR of the submandibular gland, and the MSR of the submandibular gland were significantly lower than those before treatment(T₀) in the two groups(P<0.05). At the time points of T₁ and T₂, the MAR of the parotid gland, the MSR of the parotid gland, the MAR of the submandibular gland, and the MSR of the submandibular gland in the observation group were significantly higher than those in the control group(P<0.05). The EQ-5D-5L scores in the two groups at the time points of T₁ and T₂ were significantly higher than those at the time point of T₀(P<0.05). The EQ-5D-5L scores at the time points of T₁ and T₂ in the observation group were significantly higher than those in the control group(P<0.05). The incidence of adverse reactions in the observation group was significantly lower than that in the control group(P<0.05). After the treatment, the 5-year survival rate was 89.66% in the observation group, and 75.86% in the control group. The survival prognosis of the observation group was significantly better than that of the control group(log-rank test: χ²=3.867, P=0.035). Conclusion　Compared with conventional CT-guided implantation of ¹²⁵I seeds, SPECT-CT-guided implantation of ¹²⁵I seeds has a definite therapeutic effect in treatment of patients with RAIR-DTC. The SPECT-CT-guided implantation of ¹²⁵I seeds can effectively improve the salivary gland function, quality of life and survival prognosis of the patients.]]> 2025/9/30 10:04:06 YUAN Hui, RUAN Yi 176true ［Abstract］　Objective　To investigate mechanisms of Danggui Shaoyao Powder in treating premature ovarian insufficiency(POI). Methods　The rat model of POI was constructed by intraperitoneal injection of cyclophosphamide(CTX). The blank group, model group, gonadotropin-releasing hormone analogue(GnRHa) group(with intraperitoneal injection of GnRHa at 0.1 mg/kg on the 10th and 5th days before constructing the model) and Danggui Shaoyao Powder group(with gavage administration of Danggui Shaoyao Powder at 10 mL/kg after constructing the model) were set up, with 6 rats in each group, and continuous intervention for 14 days. The morphological structure of rat ovarian tissues was observed by using hematoxylin-eosin(HE) staining and transmission electron microscopy. The levels of serum follicle-stimulating hormone(FSH), estradiol(E₂), and anti-Müllerian hormone(AMH) were detected by using enzyme-linked immunosorbent assay(ELISA). The expression levels of key factors in the transforming growth factor β1(TGFβ1)-Smad2 signaling pathway in ovarian tissues were detected by using Western blot assay and reverse transcription-polymerase chain reaction(RT-PCR) assay. Results　In the rats of the model group, the levels of serum E₂ and AMH were decreased significantly, while the level of FSH was increased significantly, with an increase in atretic follicles, granulosa cells aligned loosely, severe edema of organelles, disordered tissue spaces, cell membrane damage, and irregular depression of cell nuclei in ovarian tissues, indicating that the ovarian function was severely impaired. After the interventions with Danggui Shaoyao Powder and GnRHa, the levels of serum E₂ and AMH in the POI model rats were rebounded, while the level of FSH was decreased, with reduced vacuoles in ovarian tissues, increased ovarian follicles at all levels, damages to cell membrane and nuclear membrane similar to those in the model group, and alleviated swelling of organelles. The expression levels of phosphorylated transforming growth factor β receptor 1(p-TGFβR1) and phosphorylated Smad2(p-Smad2) protein in the ovarian tissues of the model group were significantly increased(P<0.05), and the expression levels of TGFβ1, transforming growth factor β receptor 1(TGFβR1), transforming growth factor β receptor 2(TGFβR2), Smad2 protein and mRNA were significantly increased(P<0.05), and the expression levels of Smad6 and Smad7 proteins and mRNA were significantly decreased(P<0.05), indicating that the TGFβ1-Smad2 signaling pathway was overactivated. After the intervention with Danggui Shaoyao Powder, the expression levels of Smad2 and p-Smad2 proteins were significantly decreased(P<0.05), and the expression levels of TGFβ1, TGFβR1, TGFβR2 proteins and mRNA were significantly decreased(P<0.05). The expression levels of Smad6 and Smad7 proteins and mRNA were significantly increased(P<0.05). The indicator changes after the intervention of Danggui Shaoyao Powder were similar to those after the intervention of GnRHa. Conclusion　Danggui Shaoyao Powder may exert its therapeutic effect on POI by inhibiting the abnormal activation of the TGFβ1-Smad2 signaling pathway.]]> 2025/8/29 20:37:53 WANG Jing, ZHAO Rui, CHEN Yang, ZHANG Xiaojuan, XIA Xiaoyan 175true ［Abstract］　Objective　To analyze the application effect of carotid endarterectomy(CEA) combined with endovascular therapy in patients with Hasan classification type A or B chronic internal carotid artery occlusion(CICAO). Methods　A retrospective analysis was conducted on the clinical data of 34 patients with Hasan classification type A or B CICAO who were admitted to Department of Neurosurgery, the First Teaching Hospital of Xinjiang Medical University from June 2020 to June 2024. All the patients underwent CEA combined with endovascular therapy. The recanalization of the occluded blood vessels, the occurrence of perioperative complications and recovery of neurological function were observed. Results　In the 34 patients, the occluded blood vessels were successfully recanalized in 28 patients, with a recanalization rate of 82.35%. The recanalization rate of the patients with Hasan classification type A was 89.47%(17/19), and that of the patients with Hasan classification type B was 73.33%(11/15). There was no statistically significant difference in the recanalization rate between the patients with Hasan classification type A and the patients with Hasan classification type B(P>0.05). In the 28 patients with successful recanalization of the occluded vessels, perioperative complications occurred in 6 patients, including 3 cases of Hasan classification type A and 3 cases of Hasan classification type B. In the 28 patients with successful recanalization of the occluded vessels, 25 patients had good prognosis, including 14 cases of type A and 11 cases of type B, and 3 patients had poor prognosis, all of whom were the patients with Hasan classification type A. There were 2 cases of postoperative vascular restenosis, both of whom were the patients with Hasan classification type A. In 6 patients with failed recanalization of the occluded vessels, there were 2 cases of type A and 4 cases of type B. Conclusion　The success rate of CEA combined with endovascular therapy is relatively high in patients with Hasan classification type A or B CICAO, with less perioperative complications, good neurological function recovery and vessel patency after operation. CEA combined with endovascular therapy is a safe and effective treatment method.]]> 2025/8/29 0:00:00 ZHANG Xiaojie, MAIMAITILI Aisha, KAHEERMAN Kadeer, WANG Kai, NIZHAMIDINGJIANG Rexiati, JIANG Shihao, GUO Ke, CHENG Xiaojiang 174true ［Abstract］　Objective　To summarize the clinical data of 5 patients with papillary thyroid carcinoma(PTC) who underwent da Vinci robot-assisted thyroidectomy via combining transoral vestibular approach with bilateral axillo-breast approach(BABA), and to observe the clinical effectiveness of the combined operation. Methods　Five PTC patients with clinical stage of cN_1b and cervical lymph node metastasis(LNM) who were admitted to Department of General Surgery, Tangdu Hospital, Air Force Medical University from March 2023 to March 2024 were selected. The patients underwent thyroidectomy and lymph node dissection in the areas of Ⅳ, Ⅴ and Ⅵ of the neck via transoral robotic thyroidectomy vestibular approach(TORTVA), and then lymph node dissection in the areas of Ⅱ and Ⅲ of the neck was performed through BABA. The intraoperative indicators, postoperative complications and cosmetic effects were analyzed. Results　The operations in all five patients were successful, and the operation time was (444.6±82.9)min. In central neck dissection, the number of resected lymph nodes and the number of positive lymph nodes were (15.6±8.6) and (5.0±2.0)nodes, respectively. In lateral neck dissection, the number of resected lymph nodes and the number of positive lymph nodes were (20.4±8.3) and (3.0±1.4)nodes, respectively. Two patients experienced mild salivation after operation, which healed spontaneously about 1 month later. None of the patients had hematoma or obvious subcutaneous emphysema after operation. One week after operation, the cosmetic satisfaction scores of the patients were (4.6±0.5)points, and the patients were satisfied with the cosmetic effect. Conclusion　Combining transoral vestibular approach with BABA for da Vinci robot-assisted thyroidectomy is safe and effective in treatment of PTC, with good cosmetic effect and less postoperative complications.]]> 2025/8/29 20:37:53 YANG Xiaozhou, YANG Xiaojun, HE Xianli, TANG Haili, ZHAO Huadong 173true ［Abstract］　Objective　To analyze the expressions of acidic leucine-rich nuclear phosphoprotein 32 family member B(ANP32B) and RAS oncogene family member 10(RAB10) in bone marrow of pediatric patients with acute myeloid leukemia(AML) and their relationship with clinical characteristics and survival prognosis. Methods　A total of 96 pediatric patients with AML who were admitted to Shanxi Children′s Hospital from March 2019 to March 2021 were recruited as AML group. Other 50 children who underwent bone marrow puncture for the examination of other diseases during the same period and were excluded from malignant hematological diseases were selected as control group. The levels of ANP32B and RAB10 in bone marrow were detected by using quantitative polymerase chain reaction(qPCR). The therapeutic effect was evaluated after the end of the first course of treatment. The survival prognosis of the pediatric patients with AML during the 3-year follow-up period was recorded. Cox regression was used to analyze the influencing factors of survival prognosis in the pediatric patients with AML. Results　The ANP32B level in the AML group was significantly higher than that in the control group［(3.12±0.43) vs (1.05±0.22), t=31.898, P<0.001］, and the RAB10 level in the AML group was significantly higher than that in the control group［(2.85±0.37) vs (0.95±0.21), t=33.569, P<0.001］. The levels of ANP32B and RAB10 in the pediatric patients with AML who did not achieve complete remission in the first course of treatment and were stratified as high risk were significantly increased(P<0.05). The follow-up time of the pediatric patients was 4-36(28.21±4.16)months, and their 3-year overall survival rate was 66.67%. The survival prognosis of the ANP32B low-expression group(≤3.12, 50 cases) was significantly better than that of the ANP32B high-expression group(>3.12, 46 cases)(log-rank test: χ²=9.420, P=0.001). The survival prognosis of the RAB10 low-expression group(≤2.85, 49 cases) was significantly better than that of the RAB10 high-expression group(>2.85, 47 cases)(log-rank test: χ²=19.720, P<0.001). The survival prognosis of the ANP32B+RAB10 low co-expression group was significantly better than that of the ANP32B+RAB10 high co-expression group(log-rank test: χ²=56.590, P<0.001). The results of multivariate Cox regression analysis showed that risk stratification as high risk, non-complete remission in the first course of treatment, high expression of ANP32B, and high expression of RAB10 were independent risk factors for death in the pediatric patients with AML(P<0.05). Conclusion　The levels of ANP32B and RAB10 in the bone marrow of pediatric patients with AML are elevated, and the levels are related to risk stratification, whether there is complete remission in the first course of treatment, and survival prognosis.]]> 2025/8/29 20:37:53 1, YANG Chao², ZHANG Xiaodong², ZHANG Xianhui³]]> HE Minhui¹, YANG Chao², ZHANG Xiaodong², ZHANG Xianhui³ 172true ［Abstract］　Objective　To observe the efficacy of transcutaneous electrical acupoint stimulation(TEAS) using a medium-frequency electrotherapy device combined with intratympanic injection of methylprednisolone for treatment of sudden sensorineural hearing loss(SSNHL). Methods　A total of 120 patients with SSNHL who were admitted to Department of Otolaryngology, Suzhou Hospital of Anhui Medical University from June 2023 to November 2024 were recruited. The patients were divided into control group and observation group by using random number table method, with 60 patients in each group. The control group received intratympanic injection of methylprednisolone for treatment, while the observation group received TEAS using a medium-frequency electrotherapy device combined with intratympanic injection of methylprednisolone for treatment. The treatment courses in both groups were 4 weeks. The clinical efficacy, average hearing threshold, Tinnitus Disability Scale scores, Vertigo Rating Scale scores, results of speech-recognition test, hemorheological indicators and occurrence of adverse reactions were compared between the two groups. Results　The total clinical effective rate of the observation group was significantly higher than that of the control group, with statistically significant difference between the two groups(95.00% vs 83.33%; χ²=4.227, P=0.040). After 4 weeks of treatment, the average hearing threshold, Tinnitus Disability Scale scores, Vertigo Rating Scale scores, high-shear whole blood viscosity, low-shear whole blood viscosity, plasma viscosity, hematocrit and erythrocyte aggregation index in the observation group were lower than those in the control group, and the correct rate of speech recognition and erythrocyte deformation index in the observation group were higher than those in the control group, with statistically significant differences between the two groups(P<0.05). There was no statistically significant difference in the total incidence of adverse reactions between the two groups(P>0.05). Conclusion　TEAS using a medium-frequency electrotherapy device combined with intratympanic injection of methylprednisolone is significantly effective in treatment of SSNHL. It can effectively improve the hearing level and hemorheological indicators, relieve vertigo symptoms, and has good safety.]]> 2025/8/29 0:00:00 1, TIAN Chunhui², LIU Hongfeng¹]]> ZHANG Yameng¹, TIAN Chunhui², LIU Hongfeng¹ 171true ［Abstract］　Objective　To explore the correlation of expression levels of serum spalt-like transcription factor 4(SALL4) and homeobox B7(HOXB7) in myelodysplastic syndrome(MDS) patients with their disease severity and prognosis. Methods　A total of 116 patients with MDS who were admitted to Handan Central Hospital from March 2019 to March 2023 were recruited as MDS group, and 120 healthy individuals who underwent physical examinations during the same period were selected as control group. According to the Revised International Prognostic Scoring System(IPSS-R) scores, the MDS patients were divided into low-intermediate-risk group(≤4.5 points, 45 cases), high-risk group(>4.5-6.0 points, 37 cases), and very high-risk group(>6.0 points, 34 cases). The expression levels of serum SALL4 and HOXB7 in the patients were detected by using enzyme-linked immunosorbent assay(ELISA). Multivariate Cox regression was used to analyze the factors influencing the survival prognosis of the patients. The efficacy of serum SALL4 and HOXB7 in predicting the survival of the MDS patients within 1 year after the onset of the disease was analyzed by using receiver operating characteristic(ROC) curve. Results　The levels of serum SALL4 and HOXB7 in the MDS group were significantly higher than those in the control group(P<0.05). In the MDS patients, compared with those in the low-intermediate-risk group, the levels of serum SALL4 and HOXB7 in the high-risk group and the very high-risk group were significantly increased(P<0.05), and the levels of serum SALL4 and HOXB7 in the very high-risk group were higher than those in the high-risk group, and the differences were statistically significant(P<0.05). The results of multivariate Cox regression analysis showed that higher IPSS-R scores［HR(95%CI)=3.325(1.288-8.586)］ and levels of serum SALL4［HR(95%CI)=3.684(1.598-8.491)］ and HOXB7［HR(95%CI)=3.561(1.393-9.106)］ were independent risk factors for promoting death in patients with MDS(P<0.05). The results of ROC curve analysis showed that both serum SALL4 and HOXB7 could effectively predict the survival prognosis of the patients(P<0.05), and the predictive efficacy of combination of the two indicators［AUC(95%CI)=0.920(0.854-0.962)］ was higher than that of a single indicator, with a sensitivity of 80.00% and a specificity of 88.73%. Conclusion　The levels of serum SALL4 and HOXB7 increase in the patients with MDS, which are closely related to the disease progression and survival prognosis of the MDS patients.]]> 2025/8/29 20:37:53 ZHANG Weiwei, JIAO Lihui, CHAI Lijuan, ZHANG Jing, WANG Jie, LYU Junnan, LI Zhihua, MA Xiaojun 170true ［Abstract］　Objective　To observe the effect of free-drinking water training combined with low-frequency pulse electrical stimulation in treatment of post-stroke dysphagia(PSD). Methods　One hundred and twelve patients with PSD who were admitted to the Central Hospital of Wuhan from March 2022 to March 2024 were recruited and divided into observation group and control group by random number table method, with 56 patients in each group. In addition to the conventional rehabilitation training methods such as ice water stimulating the soft palate and tongue root, perioral muscle training, respiratory function training and pronunciation training, the control group was treated with low-frequency pulse electrical stimulation, while the observation group was treated with free-drinking water training on the basis of the same treatment as the control group. The treatment course was 2 months in both groups. The results of water swallow test(WST), Dysphagia Handicap Index(DHI) scores, Eating Disorder Quality of Life Scale(EDQoL) scores, Barthel Index scores and Eating Assessment Tool-10(EAT-10) scores were compared between the two groups. The anterior displacement distance of the hyoid bone, the upward displacement distance of the hyoid bone, the anterior displacement distance of the thyroid cartilage and the upward displacement distance of the thyroid cartilage were measured by using video-fluoroscopic swallowing study(VFSS). The levels of serum brain-derived neurotrophic factor(BDNF), substance P(SP), neuron-specific enolase(NSE), nerve growth factor(NGF) and S100β protein were determined by using enzyme-linked immunosorbent assay(ELISA). Results　The effective rate of rehabilitation therapy in the observation group was significantly higher than that in the control group(85.71% vs 69.64%; χ²=4.171, P=0.041). After the rehabilitation therapy, the scores of the emotional, physiological and functional dimensions of DHI, as well as the EDQoL scores and EAT-10 scores in the observation group were significantly lower than those in the control group(P<0.05), and the Barthel index scores in the observation group were significantly higher than those in the control group(P<0.05). After the rehabilitation therapy, the anterior displacement distance of the thyroid cartilage, the upward displacement distance of the thyroid cartilage, the anterior displacement distance of the hyoid bone, the upward displacement distance of the hyoid bone in the observation group were significantly greater than those in the control group(P<0.05). After the rehabilitation therapy, the levels of serum BDNF, SP and NGF in the observation group were higher than those in the control group, while the levels of S100β protein and NSE in the observation group were lower than those in the control group, with statistically significant differences between the two groups(P<0.05). Conclusion　Free-drinking water training combined with low-frequency pulse electrical stimulation can effectively improve the patients′ PSD, promote the recovery of neurological function, and improve their living quality.]]> 2025/8/29 20:37:53 ZHONG Xiuwen, YOU Fei, MA Zhaoyang, XIAO Pei, ZHENG Jinkun, LIU Lian, CHEN Qiao 169true ［Abstract］　Objective　To compare the effectiveness, safety and economy of tenofovir disoproxil fumarate(TDF) and other oral nucleos(t)ide analogues(NAs) in blocking mother-to-child transmission of hepatitis B virus(HBV) by using rapid health technology assessment(HTA) method, and to provide reference for rational administration of medication in clinic. Methods　The English databases including PubMed, Embase and the Cochrane Library, and the Chinese databases including China National Knowledge Infrastructure(CNKI), Wanfang Med Online and VIP were systematically searched. At the same time, the official websites and related databases of HTA agencies at home and abroad were searched. The comprehensive HTA reports, systematic review(SR)/Meta-analysis and pharmaceutical economics(PE) studies on the clinical effectiveness and safety of TDF blocking mother-to-child transmission of HBV were summarized and analyzed. Results　A total of 15 papers were included, including 11 papers on SR/Meta analysis and 4 papers on PE studies. The results of effectiveness evaluation showed that TDF was superior to lamivudine(LAM) and telbivudine(LDT) in the effectiveness indicators of inhibiting maternal HBV replication and preventing mother-to-child transmission of HBV. The results of safety evaluation showed that there was no significant difference in the incidence of adverse reactions between TDF and LAM as well as between TDF and LDT(P>0.05). The economic evaluation revealed that TDF had economic advantages in China and South Africa. Conclusion　The effectiveness of TDF in blocking mother-to-child transmission of HBV is superior to that of LAM and LDT, and the safety of TDF is comparable to that of the other NAs. TDF has economic advantages in China.]]> 2025/8/29 20:37:53 TAN Sitao, LIU Xiaoxia, HUANG Hai, ZHANG Li, HE Ying, CHEN Xiaoyu 168true ［Abstract］　Objective　To explore the application value of collateral circulation grading based on magnetic resonance dynamic susceptibility contrast-enhanced perfusion-weighted imaging(DSC-PWI) raw images in patients with acute anterior circulation large vessel occlusive cerebral infarction. Methods　The clinical data of 114 patients with acute cerebral infarction in the supplying area of the middle cerebral artery caused by unilateral anterior circulation large vessel occlusion who were admitted to Wuzhou Gongren Hospital from January 2020 to April 2024 were retrospectively analyzed. All the patients were diagnosed through head magnetic resonance angiography(MRA), among whom 76 patients received digital subtraction angiography(DSA) examination. The consistency of the results of collateral circulation grading obtained by two evaluators was analyzed. The consistency of the results of collateral circulation grading assessed by using DSC-PWI raw images and DSA was analyzed. The correlation between collateral circulation assessed by using DSC-PWI raw images with the perfusion deficit volume, infarct core volume, and ratio of the mismatch area between the perfusion deficit volume and the infarct core volume(mismatch ratio) was compared. Results　There was good consistency in the assessment of collateral circulation grading results based on DSC-PWI raw images by 2 assessors(Kappa=0.821, P<0.001). There was good consistency in the assessment of collateral circulation grading results based on DSC-PWI raw images and DSA(Kappa=0.801, P<0.001). The perfusion deficit volume and infarct core volume in the good collateral circulation group were smaller than those in the poor collateral circulation group, and the mismatch ratio in the good collateral circulation group was greater than that in the poor collateral circulation group, with statistically significant differences between the two groups(P<0.05). The results of Spearman rank correlation analysis showed that collateral circulation grading based on DSC-PWI raw images was negatively correlated with the infarct core volume(r_s=-0.613, P<0.001), perfusion deficit volume(r_s=-0.382, P<0.001), and was positively correlated with the mismatch ratio(r_s=0.320, P<0.001). Conclusion　DSC-PWI raw images can be used to assess the collateral circulation of acute anterior circulation large vessel occlusive cerebral infarction.]]> 2025/8/29 0:00:00 1, XIE Jinlan¹, CHE Jinlian², MENG Yinying¹, CHEN Weihua¹]]> YAN Da¹, XIE Jinlan¹, CHE Jinlian², MENG Yinying¹, CHEN Weihua¹ 167true ［Abstract］　Objective　To analyze the occurrence characteristics and changing trends of birth defects in perinatal infants in Nanning City, Guangxi from 2017 to 2023, with the aim of providing reference for scientifically preventing and controlling birth defects of perinatal infants and promoting the health of mothers and infants. Methods　The case data of birth defects reported by various midwifery institutions in Nanning City from January 2017 to December 2023 were collected for summary and analysis. Results　From 2017 to 2023, there were 720 715 perinatal infants in Nanning City, among whom 21 888 perinatal infants were born with birth defects. The incidence of birth defects was 303.70/10 000, and the overall incidence showed an increasing trend year by year(χ²=2 291.576, P<0.001). The birth defect rate of male infants was significantly higher than that of female infants(331.01/10 000 vs 272.35/10 000; χ²=209.548, P<0.001). The birth defect rate in urban areas was significantly higher than that in rural areas(591.01/10 000 vs 176.05/10 000; χ²=8 976.240, P<0.001). The age of the delivery women was significantly associated with the occurrence of birth defects of the perinatal infants(χ²=203.659, P<0.001). From 2017 to 2023, congenital heart diseases, polydactyly and other malformations of the outer ear ranked the top 3 birth defect rates of the perinatal infants, and their incidence rates were on the rise. From 2020 to 2022, the incidence of severe thalassemia ranked among the top 10 in the incidence of birth defects of the perinatal infants. From 2017 to 2023, in Nanning City, the number of cases of major fatal and disabling birth defects had generally shown a decreasing trend, accounting for 17.13% to 39.24% of the birth defects of all the perinatal infants. Conclusion　The incidence of perinatal birth defects in Nanning City from 2017 to 2023 showed an upward trend, which may be related to the change in the age structure of the reproductive population and the implementation of screening services. It is suggested that health education be actively carried out, the management system for the prevention and control of birth defects in the whole city be improved, and the quality of the newborn population be enhanced.]]> 2025/8/29 20:37:53 MAO Liangqin, PENG Yuanjun, TAO Xing, HUANG Yongquan, LIANG Mingzhen, XIE Bingyan, WEI Jinlu, WEI Mingxiao, JIANG Wu, HE Ningyu 166true ［Abstract］　Objective　To analyze the clinical features and influencing factors of ulcerative colitis(UC) complicated with cytomegalovirus(CMV) infection. Methods　The clinical data of 228 patients with UC who were admitted to Department of Gastroenterology, General Hospital of Ningxia Medical University from January 2018 to June 2024 were retrospectively analyzed. The patients were divided into CMV-positive group(22 patients) and CMV-negative group(206 patients) according to their CMV infection results. The general data, laboratory indicators, endoscopic manifestations, treatment plans and effects were compared between the two groups. The influencing factors of UC complicated with CMV infection were explored through multivariate logistic regression analysis. Results　The proportions of the patients with fever, receiving hormone therapy in the past 1 month, receiving antibiotic therapy in the past 1 month as well as with deep chisel-like ulcer and irregular ulcer as the morphological characteristics of mucosa in the CMV-positive group were significantly higher than those in the CMV-negative group(P<0.05). The proportion of the patients with normal mucosa in the CMV-positive group was significantly lower than that in the CMV-negative group(P<0.05). There were significant differences in disease severity and Mayo endoscopic score distribution between the two groups(P<0.05). The levels of hemoglobin(Hb) and albumin(ALB) in the CMV-positive group were lower than those in the CMV-negative group, and the levels of neutrophil-to-lymphocyte ratio(NLR), erythrocyte sedimentation rate(ESR) and C-reactive protein(CRP) in the CMV-positive group were higher than those in the CMV-negative group, with statistically significant differences between the two groups(P<0.05). There were no significant differences in the proportions of the patients receiving treatments of biological agents, immunosuppressants and surgery between the two groups(P>0.05). Compared with those in the CMV-negative group, the proportion of the patients receiving treatments of glucocorticoids in the CMV-positive group was higher, and the proportion of the patients responding effectively to the glucocorticoid treatment in the CMV-positive group was lower, with statistically significant differences between the two groups(P<0.05). The results of multivariate logistic regression analysis showed that deep chisel-like ulcer was an independent risk factor for UC complicated with CMV infection［OR(95%CI)=35.513(3.932-320.759), P=0.001］, and higher Hb level was an independent protective factor for inhibiting UC complicated with CMV infection［OR(95%CI)=0.951(0.912-0.991), P=0.016］. Conclusion　Patients with severe UC are susceptible to complication with CMV infection. Hb level and deep chisel-like ulcer observed under endoscope are significantly associated with CMV infection. Clinicians need to strengthen the monitoring of such patients and give antiviral therapy promptly to improve the prognosis.]]> 2025/7/31 13:22:15 1,2, ZHANG Li′na¹, ZHAO Guihong^1,2, SU Rong¹, YANG Jiali¹, WANG Fang¹, YANG Shaoqi¹]]> LIU Jiaxin^1,2, ZHANG Li′na¹, ZHAO Guihong^1,2, SU Rong¹, YANG Jiali¹, WANG Fang¹, YANG Shaoqi¹ 165true ［Abstract］　Objective　To analyze the clinical efficacy of Nanhu gel in treatment of rheumatoid arthritis(RA) and to identify its active ingredients. Methods　Using Franz diffusion devices with female nude mouse skin as the barrier and 75% ethanol solution as the receiving medium, thin-layer chromatography(TLC) was employed to identify active ingredients in the transdermal absorption solution of Nanhu gel. Sixty RA patients admitted to Southern Medical University Hospital of Integrated Traditional Chinese and Western Medicine from May 2017 to December 2021 were recruited and randomly divided into blank gel group, Nanhu gel group and Votalin(diclofenac sodium) group using random number table method, with 20 patients in each group. Blank gel, Nanhu gel, and Votalin cream were topically applied to the most significantly swollen joints(single joint) in the patients of each group, 0.5-1.0 g per time(adjusting the dosage according to the joint circumference), massaging the joints where the medicine was applied for 1 to 2 minutes, once daily, for 7 consecutive days. The clinical efficacy, the onset time of the medication, Visual Analogue Scale(VAS) scores, joint circumference and inflammatory indicators were compared among the three groups. The occurrence of adverse reactions in the three groups during the treatment period was recorded. Results　The results of TLC identification suggested that emodin, celastrol and myrrh essential oil in Nanhu gel could exert therapeutic effects through permeating the skin. After the treatment, the VAS scores in the Nanhu gel group were significantly lower than those in the Votalin group and the blank gel group(P<0.05), and the onset time of the medication in the Nanhu gel group was significantly faster than that in the Votalin group and the blank gel group(P<0.05). The total clinical effective rates in the Nanhu gel group, the Votalin group and the blank gel group were 90.00%, 80.00% and 70.00%, respectively, and the differences were statistically significant among the three groups(P<0.05). There were no statistically significant differences in the joint circumference, erythrocyte sedimentation rate(ESR) and C-reactive protein(CRP) levels among the three groups before and after treatment(P>0.05). No drug-related adverse reactions were observed in the three groups during the treatment period. Conclusion　Nanhu gel can effectively relieve the clinical symptoms of RA patients and has the advantages of good safety and rapid onset of action. Emodin, celastrol and myrrh essential oil are its main active ingredients.]]> 2025/7/31 0:00:00 1,2, WANG Yuyu¹, WANG Jin¹, ZHAO Xiaofeng¹, ZUO Fangfang¹, XUAN Ya′nan¹, CHEN Ensheng¹, XIAO Changhong^1,2, LIU Qiang²]]> YUAN Yi^1,2, WANG Yuyu¹, WANG Jin¹, ZHAO Xiaofeng¹, ZUO Fangfang¹, XUAN Ya′nan¹, CHEN Ensheng¹, XIAO Changhong^1,2, LIU Qiang² 164true ［Abstract］　Objective　To analyze the clinicopathological characteristics and survival of 85 patients with mesothelioma in a single center and to explore the influencing factors of prognosis in the mesothelioma patients. Methods　The clinicopathological data of 85 patients with mesothelioma who were admitted to Liaoning Cancer Hospital and Institute from April 2003 to April 2021 were retrospectively analyzed. Results　Among the 85 patients with mesothelioma, there were 42 males and 43 females, with the main onset age ranging from 60 to 70 years. There were 60 cases of pleural mesothelioma and 25 cases of peritoneal mesothelioma. There was a correlation between diseased site and histopathological type(χ²=9.671, P=0.008). There were 56 cases of epithelioid mesothelioma, 9 cases of sarcomatoid mesothelioma, and 20 cases of biphasic mesothelioma. There were 18 patients with lymph node metastasis and 67 patients without lymph node metastasis. The histopathological subtypes of 56 cases of epithelioid mesothelioma were mainly tubular papillary(53.57%), including 29 cases(51.79%) of low-grade epithelioid mesothelioma and 27 cases(48.21%) of high-grade epithelioid mesothelioma. There was a correlation between histopathological grading of epithelioid mesothelioma and lymph node metastasis(χ²=5.589, P=0.018). The overall survival of 85 patients with mesothelioma ranged from 3 to 32 months, with a median survival time of 16.0 months. The 1-year overall survival rate was 70.10%, and the 2-year overall survival rate was 36.50%. There were significant differences in survival prognosis among the mesothelioma patients with different histopathological types and different treatment methods(P<0.05). The mesothelioma patients without lymph node metastasis had significantly better survival prognosis than those with lymph node metastasis(P<0.05). Among the patients with pleural mesothelioma, the patients without lymph node metastasis had significantly better survival prognosis than those with lymph node metastasis(P<0.05), and there was significant difference in survival prognosis among the patients treated with different methods(P<0.05). In the patients with peritoneal mesothelioma, there was significant difference in survival prognosis among the patients with different histopathological types and there was significant difference in survival prognosis among the patients treated with different methods(P<0.05). Conclusion　Pleural mesothelioma is more common than peritoneal mesothelioma, with epithelioid mesothelioma being the main type and sarcomatoid mesothelioma being the least. Patients with epithelioid mesothelioma have a better survival prognosis, while patients with sarcomatoid mesothelioma have a poorer survival prognosis, and patients with lymph node metastasis have a poorer prognosis. The therapeutic effect of surgery combined with chemotherapy is relatively good. Epithelioid mesothelioma has diverse histopathological subtypes, and the tubular papillary subtype is in the majority, with similar proportions of high-grade and low-grade cases. The patients with low-grade epithelioid mesothelioma have a longer median survival time than those with high-grade epithelioid mesothelioma.]]> 2025/7/31 13:22:15 1,2,3, LIU Man³, HUANG Bo²]]> MA Siyuan^1,2,3, LIU Man³, HUANG Bo² 163true ［Abstract］　Objective　To explore the restorative effect of strontium-loaded nanotube-based pure titanium(Ti) implants on alveolar bone defects. Methods　Thirty 3-month-old Sprague-Dawley(SD) rats were selected for experiment and were randomly divided into blank group, nanotube group and strontium-loaded nanotube group, with 10 rats in each group. Smooth cylindrical implants without screw threads(with a diameter of 4.5 mm and a length of 8 mm) were made of pure Ti. The implants in the three groups were treated differently. For the implants in the blank group, no treatment was given after conventional polishing. The implants of the nanotube group were treated using hydrofluoric acid solution with a mass fraction of 0.5%, with graphite as the cathode and the implants as the anode, and anodic oxidation treatment at a voltage of 20 V for 1 hour. The implants of the strontium-loaded nanotube group were treated with 0.02 mol/L strontium hydroxide solution in a sealed water bath at 200 ℃ for 1 hour on the basis of the treatment of the nanotube group. The incisors of the left mandible of the rats were extracted, and the prepared alveolar sockets were rinsed with normal saline. The implants were placed in the alveolar sockets about 8 mm deep from the edge of the gingiva, approximately at the position of the root of the first molar. Then, the wound was sutured using 5-0 absorbable sutures. Five rats in each group were sacrificed at the 4th and 12th weeks after operation, respectively, and mandibular specimens were taken for detection. The elemental composition of the implant surface was analyzed using an energy spectrum analysis tool of Energy Dispersive Spectrometer built in the electron microscope. The surface roughness of the implants was detected by using atomic force microscopy. Micro-computed tomography, Masson staining and immunohistochemistry staining were used for histological evaluation. Results　Compared with those in the blank group, the Ti content detected on the surface of the implants was decreased, while the oxygen(O) content and fluorine(F) content detected on the surface of the implants were increased in the nanotube group and the strontium-loaded nanotube group. The F content detected on the surface of the implants in the strontium-loaded nanotube group was higher than that in the nanotube group. Compared with that in the blank group, the surface roughness of the implants in the nanotube group and the strontium-loaded nanotube group was increased significantly(P<0.05), and the surface roughness of the strontium-loaded nanotube group was greater than that of the nanotube group(P<0.05). Compared with those in the blank group, bone volume/total volume(BV/TV), bone surface/bone volume(BS/BV) and trabecular thickness(Tb.Th) in the nanotube group and the strontium-loaded nanotube group were significantly increased at the 4th week after operation(P<0.05). At the 12th week after operation, compared with those in the blank group and the nanotube group, BV/TV, BS/BV and Tb.Th in the strontium-loaded nanotube group were significantly increased, while there were no statistically significant differences in BV/TV, BS/BV and Tb.Th between the nanotube group and the blank group(P>0.05). At the 4th and 12th weeks after operation, there were no statistically significant differences in trabecular number(Tb.N) and trabecular spacing(Tb.Sp) among the three groups(P>0.05). The Masson staining results showed that a large amount of thick mature bone tissues were formed around the bone defect area of the strontium-loaded nanotube group, while the nanotube group and the blank group had less bone mass and lacked integrity in the formation of new bones. At the 12th week after operation, compared with those in the blank group, the positive staining areas of osteopontin(OPN) and bone morphogenetic protein 2(BMP2) in the bone defect site of the rats in the nanotube group and the strontium-loaded nanotube group were increased, and the positive staining areas of OPN and BMP2 in the bone defect site of the rats in the strontium-loaded nanotube group were larger than those in the nanotube group, with statistically significant differences(P<0.05). Conclusion　Strontium-loaded nanotube-based pure Ti implants can promote the restorations in alveolar bone defects.]]> 2025/7/31 13:22:43 1, SONG Liye², CHENG Bingkun³, TIAN Xiaomei¹, LIU Yingqi¹]]> HE Jiahuan¹, SONG Liye², CHENG Bingkun³, TIAN Xiaomei¹, LIU Yingqi¹ 162true ［Abstract］　Objective　To analyze the clinical data of 176 pediatric patients with central airway stenosis and to summarize the etiological distribution and clinical features of central airway stenosis in the pediatric patients, providing reference for the diagnosis and treatment of central airway stenosis in pediatric patients. Methods　The clinical data of 176 pediatric patients with central airway stenosis who were admitted to the First Affiliated Hospital of Guangzhou Medical University from January 2018 to December 2023 were retrospectively analyzed. The clinical data included the pediatric patients′ general data, causes and locations of central airway stenosis, results of the first pulmonary function test, and results of computed tomography(CT) and bronchoscopy during their hospitalization, and results of blood gas analysis and clinical manifestation at admission. Results　Among the 176 pediatric patients with central airway stenosis, 120 pediatric patients were male(68.18%) and 56 pediatric patients were female(31.82%). The median age was 17 months. The main causes of congenital central airway stenosis(25.00%, 44/176) were congenital pulmonary airway malformation(18.18%, 32/176), among them, the pediatric patients aged 12-35 months accounted for the highest proportion(37.50%, 12/32). The main causes of acquired central airway stenosis(75.00%, 132/176) were tracheal intubation or tracheotomy(35.23%, 62/176), airway foreign body(17.05%, 30/176) and airway infection(15.91%, 28/176) in sequence, among them, the proportion of the pediatric patients aged ≥36 months who underwent tracheal intubation or tracheotomy was the highest(43.55%, 27/62). The subglottic stenosis(39.20%, 69/176) and the stenosis in the right main bronchus or the right middle bronchus, and the left main bronchus(37.50%, 66/176) accounted for a relatively high proportion. The main cause of subglottic stenosis was tracheal intubation or tracheotomy(78.26%, 54/69). Pulmonary ventilation function test was performed on 7 pediatric patients, and the results indicated obstructive ventilation dysfunction in varying degrees. Blood gas analysis was performed on 109 pediatric patients, and the results showed that partial pressure of oxygen(PaO₂) decreased in 44 cases(40.37%), partial pressure of carbon dioxide(PaCO₂) increased in 35 cases(32.11%), pH decreased in 29 cases(26.61%), actual bicarbonate ion concentration increased in 38 cases(34.86%), and base excess increased in 22 cases(20.18%). The main clinical manifestations of the pediatric patients were cough, wheezing, shortness of breath and three-concave sign. The detection rates of central airway stenosis by using CT and using bronchoscopy were 80.46% and 100.00%, respectively. Conclusion　The proportions of the pediatric patients with different etiologies in different age groups and the main causes of each stenosis location in the central airway are different. The main causes of acquired central airway stenosis are tracheal intubation or tracheotomy, airway foreign body and airway infection in sequence.]]> 2025/7/31 0:00:00 TANG Sixiang, WU Shangzhi, LI Hongwei, LIN Junxing, XU Qingyun, LIN Yuneng, XU Jiaxing, LONG Jitong, HUANG Zhanhang, CHEN Dehui 161true ［Abstract］　Objective　To observe the effect of estrogen combined with Zhuang acupuncture therapy in treatment of thin endometrium on frozen-thawed embryo transfer for assisted pregnancy. Methods　A total of 137 patients with thin endometrium who received frozen-thawed embryo transfer for assisted pregnancy in Center for Reproductive Medicine and Genetics, the People′s Hospital of Guangxi Zhuang Autonomous Region from March 2022 to June 2024 were recruited. The patients were divided into control group(receiving conventional estrogen treatment regimen for preparing the endometrium, 69 patients) and observation group(receiving conventional estrogen treatment regimen plus Zhuang acupuncture therapy for preparing the endometrium, 68 patients). During the treatment process, 9 patients were excluded from the control group and 8 patients from the observation group. Eventually, 60 patients were included in each group for the analysis. The endometrial thickness, the grade of endometrial blood flow, serum estradiol(E₂) and progesterone(P) levels, and pregnancy outcomes were compared between the two groups. The occurrence of adverse reactions was recorded. Results　After the treatment, the endometrium in the observation group was thicker than that in the control group, and the difference was statistically significant(P<0.05). After the treatment, the endometrial blood flow grade in the observation group was significantly better than that in the control group(P<0.05). There were no statistically significant differences in the levels of serum E₂ and P between the two groups before and after the treatment(P>0.05). The implantation rate and clinical pregnancy rate in the observation group were higher than those in the control group, and the differences were statistically significant(P<0.05). No serious adverse reactions occurred in all the patients during the treatment period. Conclusion　Estrogen combined with Zhuang acupuncture therapy can effectively improve endometrial thickness and increase clinical pregnancy rate.]]> 2025/7/31 13:22:43 1, LIU Liling¹, QIN Jie¹, HUANG Taishuai¹, HE Zhou²]]> CHI Yan¹, LIU Liling¹, QIN Jie¹, HUANG Taishuai¹, HE Zhou² 160true ［Abstract］　Objective　To explore the expressions of serum 8-hydroxy-2′-deoxyguanosine(8-OHdG) and stromal cell-derived factor-1(SDF-1) in pediatric patients with neonatal respiratory distress syndrome(NRDS) and their clinical significance. Methods　A total of 108 pediatric patients with NRDS(NRDS group) and 52 normal neonates(control group) who were admitted to Shangluo Central Hospital from July 2021 to May 2024 were recruited. The pediatric patients with NRDS were divided into mild NRDS group(22 patients), moderate NRDS group(50 patients) and severe NRDS group(36 patients) according to their disease severity. Serum 8-OHdG and SDF-1 levels were detected by using enzyme-linked immunosorbent assay. Cox regression was used to analyze the influencing factors of prognosis in the pediatric patients with NRDS. The efficacy of 8-OHdG and SDF-1 levels in predicting death of the pediatric patients with NRDS was analyzed by using receiver operating characteristic(ROC) curve. Results　The level of 8-OHdG in the NRDS group was higher than that in the control group［(13.81±3.90)ng/mL vs (3.70±1.47)ng/mL］, and the level of SDF-1 in the NRDS group was higher than that in the control group［(12.46±1.62)ng/mL vs (8.23±1.32)ng/mL］, with statistically significant differences between the two groups(P<0.05). The levels of 8-OHdG and SDF-1 in the mild NRDS group, the moderate NRDS group and the severe NRDS group were gradually increased(P<0.05). The mortality rate of the 108 pediatric patients with NRDS within 28 days after admission was 25.00%(27/108). The results of multivariate Cox regression analysis showed that severe NRDS, and higher 8-OHdG and SDF-1 levels were independent risk factors for death in the pediatric patients with NRDS(P<0.05). The results of ROC curve analysis showed that the levels of 8-OHdG and SDF-1 were helpful for predicting death of the pediatric patients with NRDS(P<0.05). The predictive efficacy of the combination of the two indicators［AUC(95%CI)=0.925(0.859-0.967)］ was higher than that of a single indicator(P<0.05), with a sensitivity of 92.59 % and a specificity of 77.78 %. Conclusion　The elevated serum 8-OHdG and SDF-1 levels are closely related to the disease severity and the increase of death risk in pediatric patients with NRDS. The combination of the two indicators is more effective than a single indicator in predicting prognosis of the pediatric patients with NRDS.]]> 2025/7/31 0:00:00 YANG Lijun, SUN Xu′e, CHEN Tingting 159true ［Abstract］　Objective　To explore the association of serum beta-hydroxybutyrate(β-HB) and glycated hemoglobin(HbA₁c) with occurrence of cognitive dysfunction in diabetic ketoacidosis(DKA) pediatric patients complicated with cerebral edema. Methods　A total of 148 DKA pediatric patients complicated with cerebral edema who were admitted to Tangshan Central Hospital from March 2020 to March 2023 were recruited. After the pediatric patients were admitted to the hospital for treatment and their vital signs were stable, the cognitive function of the pediatric patients was evaluated by using Wechsler Intelligence Scale for Children, Fourth Edition(WISC-Ⅳ), and the pediatric patients were divided into cognitive dysfunction group(WISC-Ⅳ scores <80 points, 26 patients) and control group(WISC-Ⅳ scores ≥80 points, 122 patients) according to the evaluation results. The levels of serum β-HB and HbA₁c, and the other clinical data were compared between the two groups. The correlation of serum β-HB and HbA₁c with WISC-Ⅳ scores were analyzed by using Pearson correlation. Multivariate logistic regression was used to analyze the risk factors of cognitive dysfunction in the DKA pediatric patients complicated with cerebral edema. The efficacy of serum β-HB and HbA₁c levels in predicting cognitive dysfunction in the DKA pediatric patients complicated with cerebral edema was evaluated by using receiver operating characteristic(ROC) curve. Results　Compared with those in the control group, the levels of fasting plasma glucose(FPG), lactic acid(Lac), total cholesterol(TC), HbA₁c and β-HB in the cognitive dysfunction group were increased, while the levels of blood sodium and blood pH in the cognitive dysfunction group were decreased, with statistically significant differences between the two groups(P<0.05). The results of Pearson correlation analysis showed that the levels of serum β-HB and HbA₁c were negatively correlated with the WISC-Ⅳ scores(r=-0.634, P<0.001; r=-0.590, P<0.001). The results of multivariate logistic regression analysis showed that higher levels of β-Hb ［OR(95%CI)=2.119(1.090-4.118)］ and HbA₁c［OR(95%CI)=2.423(1.083-5.423)］ were independent risk factors for cognitive dysfunction in the pediatric patients(P<0.05), while higher blood sodium level ［OR(95%CI)=0.590(0.363-0.960)］ was an independent protective factor for inhibiting cognitive dysfunction(P<0.05). The results of ROC curve analysis showed that serum β-Hb and HbA₁c could effectively predict cognitive dysfunction in the DKA pediatric patients complicated with cerebral edema(P<0.05), and the predicting efficacy of the combined detection of the two indicators［AUC(95%CI)=0.930(0.867-0.969)］ was better than that of a single indicator, and the sensitivity and specificity were 88.46% and 91.80%, respectively. Conclusion　The levels of serum β-HB and HbA₁c are elevated in DKA pediatric patients complicated with cerebral edema, which can predict the risk of cognitive dysfunction in DKA pediatric patients complicated with cerebral edema. The prediction is helpful for clinicians to intervene early and improve the prognosis of the pediatric patients.]]> 2025/7/31 13:22:43 1, ZHANG Liguang², FAN Aihong²]]> MI Yanru¹, ZHANG Liguang², FAN Aihong² 158true ［Abstract］　Objective　To analyze the clinical characteristics and surgical treatment effects of 33 patients with thoracic giant tumors. Methods　A retrospective analysis was conducted on the clinical data of 33 patients with thoracic giant tumors who underwent surgical treatment in the Department of Thoracic Surgery of Aerospace Center Hospital from March 2015 to June 2023. The clinicopathological characteristics, selection of surgical incisions and prognosis of the patients were summarized. Results　Among the 33 patients, 11 patients were male and 22 patients were female. There were 7 asymptomatic patients and 26 symptomatic patients. The main part of the tumors was located in the left chest in 3 patients, in the right chest in 1 patient, and in the mediastinum in 29 patients. The postoperative pathological results showed that there were 19 cases of benign tumors and 14 cases of malignant tumors. The operations of 19 patients were completed through the median incision of the chest and longitudinal splitting sternums. The surgical options for 7 patients were to enter the chest through the posterolateral incision. Three patients underwent minimally invasive thoracoscopic surgery. The surgical options for 4 patients were low-collar arc-shaped incision. One patient with malignant tumor died during the perioperative period. Re-expansion pulmonary edema occurred in 2 cases after the operation. Thirteen patients with malignant tumors received adjuvant chemotherapy or radiotherapy after the operation, among whom 4 patients died due to tumor recurrence and metastasis. The patients were followed up until January 2024, and 28 patients survived healthily. Conclusion　Surgical operation is an effective method for treating thoracic giant tumors, which can make the most patients achieve good long-term outcomes. However, due to the particularity of thoracic giant tumors, thorough assessment and preparation are required before the operation. During the operation, the safety of the heart and other important organs must be ensured. After the operation, attention should be paid to the occurrence of serious complications.]]> 2025/7/31 13:22:43 REN Dewang, MIAO Libin, WANG Zhiyuan, LU Weiqiang, MA Runyang, DOU Xuejun 157true ［Abstract］　Objective　To explore the effects of proactive multifaceted thermal insulation measures on mothers and their babies in late-preterm caesarean section. Methods　Two hundred postpartum women who underwent caesarean section in the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2022 to May 2024 were recruited, with gestational age of 34⁺²～36⁺⁶(35.42±1.21) weeks. The postpartum women were divided into observation group and control group by random number table method, with 100 patients in each group. The control group adopted conventional thermal insulation measures, while the observation group adopted proactive multifaceted thermal insulation measures. The perioperative body temperature, duration of the operation, intraoperative blood loss, postoperative related indicators and the occurrence of complications of the postpartum women, and the indicators of their newborns′ condition were compared between the two groups. Results　At the beginning of anesthesia, 30 minutes after the operation, at the time of delivery and 30 minutes after returning to the ward, the body temperature of the postpartum women in the observation group was significantly higher than that in the control group(P<0.05). The intraoperative blood loss and the blood loss of the postpartum women 2 hours after delivery in the observation group were less than those in the control group. The blood oxygen saturation of the postpartum women in the observation group was higher than that in the control group 30 minutes after returning to the ward. The duration of the operation and the hospital stay of the postpartum women in the observation group were shorter than those in the control group. The ambulant time after operation and the postoperative exhaust time in the observation group was earlier than that in the control group. The differences in the above indicators were significant between the two groups(P<0.05). The rectal temperature, pH of umbilical artery blood and trace blood glucose levels of the newborns in the observation group were higher than those in the control group, with statistically significant differences between the two groups(P<0.05). The incidence rates of shivering, postpartum hemorrhage and intraoperative hypothermia in the observation group were significantly lower than those in the control group(P<0.05). Conclusion　Proactive multifaceted thermal insulation measures can reduce the incidence of hypothermia in the postpartum women undergoing caesarean section, reduce their blood loss, shorten the duration of the operation, promote rapid recovery of the postpartum women after the operation, and improve the physiological indicators of their newborns. Proactive multifaceted thermal insulation measures have clinical application value.]]> 2025/7/31 13:22:43 1, ZHONG Yunxiang², LU Yanqun¹, ZHANG Chun¹, MO Jiacheng³, LONG Bo⁴, ZHANG Shanshan¹]]> WEI Yanfen¹, ZHONG Yunxiang², LU Yanqun¹, ZHANG Chun¹, MO Jiacheng³, LONG Bo⁴, ZHANG Shanshan¹ 156true ［Abstract］　Objective　To analyze the therapeutic effect of repetitive transcranial magnetic stimulation(rTMS) combined with conventional speech rehabilitation training on post-stroke dysarthria patients. Methods　A total of 126 patients with post-stroke dysarthria who were admitted to Ruijin Hospital, Shanghai Jiao Tong University School of Medicine from June 2022 to March 2024 were recruited. The patients were divided into observation group and control group by using random number table method, with 63 patients in each group. The control group was given conventional speech rehabilitation training, while the observation group was given rTMS combined with conventional speech rehabilitation training. Both groups were treated continuously for 4 weeks.The total effective rates were compared between the two groups and the changes in articulating function, acoustic indicators and daily living ability were compared between the two groups before and after treatment. The occurrence of adverse reactions during the treatment period was recorded in both groups. Results　The total effective rate of the observation group was significantly higher than that of the control group(P<0.05). After treatment, the scores of the dimensions of reflex, breathing, lip movement, tongue movement, jaw movement, soft palate movement, laryngeal control and speech comprehension, and the total scores in the modified Frenchay Dysarthria Assessment Scale in both groups were higher than those before treatment, and the scores in the observation group were higher than those in the control group after treatment, and the differences were statistically significant(P<0.05). After treatment, the voice loudness, clarity of articulations and modified Barthel Index scores in the two groups were higher than those before treatment, and these indicators in the observation group were higher than those in the control group after treatment, and the longest sound duration in the two groups was longer than that before treatment, and the longest sound duration in the observation group was longer than that in the control group after treatment, with statistically significant differences(P<0.05). During the treatment period, neither group experienced any adverse reactions. Conclusion　The combination of rTMS and conventional speech rehabilitation training is significantly effective in treatment of patients with post-stroke dysarthria. The combined treatment can effectively improve dysarthria, prolong the longest sound duration, and improve voice loudness, clarity of articulations and daily living ability.]]> 2025/7/31 13:22:43 1, WANG Zhenni², ZHANG Weiming¹, XIAO Zihao², GAO Yi², SHEN Qin², ZHAO Na²]]> FANG Chenjun¹, WANG Zhenni², ZHANG Weiming¹, XIAO Zihao², GAO Yi², SHEN Qin², ZHAO Na² 155true ［Abstract］　Objective　To analyze the mechanism of splicing factor DEAD-box helicase 3 Y-linked(DDX3Y) promoting the proliferation of gastric cancer cells and growth of organoids from males through down-regulating IL-6/JAK/STAT3 signaling pathway. Methods　The expression levels of DDX3Y in gastric cancer tissues and their adjacent tissues, as well as the relationship between the expressions of DDX3Y and the patients′ prognosis were analyzed by using bioinformatics methods. The gastric cancer tissues and their adjacent tissues(>5 cm away from the gastric cancer tissues) of the male gastric cancer patients who were admitted to the First Affiliated Hospital of Soochow University were collected for immunohistochemical staining. Human immortalized gastric mucosal epithelial cells(GES-1) and male-derived gastric cancer cells(SNU-1, NUGC-3, KATOIII, MKN74, HGC-27 and NCI-N87) were cultured. The expression levels of DDX3Y in different cell lines were detected by using Western blot method. SNU-1 and HGC-27 cells were transfected with lentivirus to construct DDX3Y-high-expression/DDX3Y-low-expression cells(OE-DDX3Y/shDDX3Y), and EdU cell proliferation assays were performed. The organoids were established by using tumor tissues of the male gastric cancer patients, and lentiviral transfection was used to further generate organoids with DDX3Y-high-expression/DDX3Y-low-expression. The effects of inhibiting IL-6/JAK/STAT3 signaling pathway on organoid diameter and viability was analyzed. The possibility of DDX3Y binding to IL-6 precursor mRNA was predicted by using the RBPmap online website. Results　DDX3Y mRNA expression level in the adjacent cancer tissues was significantly higher than that in the gastric cancer tissues(P<0.05). The survival prognosis of the patients with high expression of DDX3Y mRNA was significantly better than that of the patients with low expression of DDX3Y mRNA(log-rank test: χ²=7.856, P=0.005). Compared with that in GES-1 cells, the expression of DDX3Y protein in HGC-27 cells was significantly higher(P<0.05). Compared with that in HGC-27 cells, expression of DDX3Y protein in SNU-1 cells was significantly lower(P<0.05). In the SNU-1 cells, the expression level of DDX3Y protein in the OE-DDX3Y group was significantly higher than that in the high expression control group(P<0.05), and the proportion of EdU staining positive cells in the OE-DDX3Y group was significantly lower than that in the high expression control group(P<0.05). In the HGC-27 cells, the expression level of DDX3Y protein in the shDDX3Y-1 group was significantly lower than that in the low expression control group(P<0.05), and the proportion of EdU staining positive cells in the shDDX3Y group was significantly higher than that in the low expression control group(P<0.05). Compared with that in the high expression control group, the organoid diameter in the OE-DDX3Y group was significantly decreased(P<0.05), and the vitality in the OE-DDX3Y group was significantly reduced(P<0.05). After inhibiting the IL-6/JAK/STAT3 signaling pathway, the organoid diameter in the OE-DDX3Y group was further decreased(P<0.05), and the vitality in the OE-DDX3Y group was further reduced(P<0.05). Compared with that in the low expression control group, the organoid diameter in the shDDX3Y group was significantly increased(P<0.05), and the vitality in the shDDX3Y group was significantly increased(P<0.05). After inhibiting the IL-6/JAK/STAT3 signaling pathway, the organoid diameter in the shDDX3Y group was significantly decreased(P<0.05), and the viability in the shDDX3Y group was significantly reduced(P<0.05). The prediction results of the RBPmap online website suggested that DDX3Y was very likely to bind to the IL-6 precursor mRNA(Z-score>3, with a significant binding signal). Conclusion　Downregulation of splicing factor DDX3Y promotes male gastric cancer proliferation and organoid growth. The alternative splicing of IL-6 regulated by the splicing factor DDX3Y can further promote tumor progression by influencing the IL-6/JAK/STAT3 signaling pathway.]]> 2025/7/4 0:00:00 ZHOU Yajing, LI Dongbao, WANG Pengbo, DUAN Kaipeng, DONG Chao, LI Weikang, SUN Xiaotong, XU Chengxiang, ZHOU Jin 154true ［Abstract］　Objective　To analyze the risk factors for poor prognosis in patients with malignant tumor-related cerebral infarction. Methods　The clinical data of 83 patients with malignant tumor-related cerebral infarction who were admitted to the First Affiliated Hospital of Guangxi Medical University from January 2012 to December 2022 were retrospectively analyzed. The patients were divided into poor prognosis group(>2 points, 43 cases) and good prognosis group(≤2 points, 40 cases) according to their modified Rankin Scale(mRS) scores at discharge. The clinical data were compared between the two groups. The factors influencing the prognosis of the patients were analyzed through multivariate logistic regression, and the efficacy of the research indicators in predicting the prognosis of the patients was analyzed by using receiver operating characteristic(ROC) curve. Results　The results of multivariate logistic regression analysis showed that higher platelet count［OR(95%CI)=0.991(0.983-0.998), P=0.019］ and serum prealbumin level［OR(95%CI)=0.986(0.975-0.998), P=0.022］ were independent protective factors for a good prognosis of malignant tumor-related cerebral infarction. The elevated systemic inflammation response index(SIRI)［OR(95%CI)=1.285(1.027-1.607), P=0.028］ was an independent risk factor for a poor prognosis of malignant tumor-related cerebral infarction. The results of ROC curve analysis showed that platelet count, serum prealbumin and SIRI could effectively predict the occurrence of poor prognosis of malignant tumor-related cerebral infarction(P<0.05), and the predictive efficacy of the combination of the three indicators was better than that of a single indicator［AUC(95%CI)=0.800(0.697-0.889)］, with a sensitivity of 74.4% and a specificity of 72.5%. Conclusion　SIRI, platelet count and serum prealbumin levels are associated with the prognosis of the patients with malignant tumor-related cerebral infarction. The combined detection of the three indicators is helpful for predicting the prognosis of the patients.]]> 2025/7/4 11:15:38 1, LIANG Huo¹, LU Qiuhong¹, HUANG Qiuhui¹, CEN Gengyu¹, LIANG Zhijian^1,2]]> WANG Xue¹, LIANG Huo¹, LU Qiuhong¹, HUANG Qiuhui¹, CEN Gengyu¹, LIANG Zhijian^1,2 153true ［Abstract］　Objective　To analyze the effect of autonomic nervous function on postoperative rehabilitation of gastric cancer patients. Methods　The clinical data of 72 patients with gastric cancer who received laparoscopic radical gastrectomy in Department of General Surgery, Affiliated Hospital of Nanjing University of Chinese Medicine(Jiangsu Province Hospital of Chinese Medicine) from November 2022 to May 2024 were retrospectively analyzed. After admission, the standard deviation of normal-to-normal intervals(SDNN) values were detected to evaluate the patients′ autonomic nervous function. According to SDNN values on the first day before surgery, the patients were divided into low SDNN group(SDNN<102 ms, 32 patients) and normal SDNN group(SDNN: 102-180 ms, 40 patients). The postoperative recovery and inflammatory indicators were compared between the two groups. Results　The time of first exhaust, the time of first consumption of liquid food, and the time of postoperative removal of drainage tube in the normal SDNN group was earlier than that in the low SDNN group, and the postoperative hospitalization days in the normal SDNN group were less than those in the low SDNN group, with statistically significant differences between the two groups(P<0.05). The Visual Analogue Scale(VAS) scores on the 2nd day and the 3rd day after surgery were lower than those on the 1st day after surgery in both groups, and the VAS scores on the 3rd day after surgery were lower than those on the 2nd day after surgery in the normal SDNN group, with statistically significant differences(P<0.05). There were no statistically significant differences in VAS scores between the two groups at different time points(P>0.05). The levels of white blood cell(WBC), high-sensitivity C-reactive protein(hs-CRP) and interleukin-6(IL-6) on the 1st day and the 3rd day after surgery were higher than those before surgery in both groups, and the levels of WBC on the 3rd day after surgery were lower than those on the 1st day after surgery in both groups, with statistically significant differences(P<0.05). The level of WBC on the 3rd day after surgery in the normal SDNN group was significantly lower than that in the low SDNN group(P<0.05). Conclusion　Good autonomic nervous function helps gastric cancer patients resist surgical stress, promotes postoperative gastrointestinal function recovery, and improves postoperative pain and inflammatory state. SDNN is expected to become a preoperative pre-rehabilitation indicator to assess the postoperative rehabilitation status of the patients and guide clinical practice.]]> 2025/7/4 0:00:00 XU Pengyan, NAN Haiou, CAI Yuling, LI Jiayu, GE Miaomiao, WANG Gang, JIANG Zhiwei 152true ［Abstract］　Objective　To analyze the expressions of proviral integration site for Moloney murine leukemia virus 2(PIM2) and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3(PFKFB3) in tissues of patients with non-small cell lung cancer(NSCLC) and the association of these expressions with clinicopathological features and survival prognosis. Methods　A total of 98 patients with NSCLC who were admitted to the First People′s Hospital of Guangyuan from January 2020 to March 2021 were selected. The clinical data of the patients were collected. During the operation, NSCLC tissues and pericarcinomatous tissues(>3 cm away from the cancer tissues) were collected. The expressions of PIM2 and PFKFB3 were detected by immunohistochemical staining. The patients were followed up for 3 years after the operation. The survival status of the patients was recorded. Cox regression was used to analyze the factors influencing the survival prognosis of the NSCLC patients. The survival curve was plotted using Kaplan-Meier method, and log-rank test was used for inter-group comparison. Results　The positive expression rates of PIM2 and PFKFB3 in NSCLC tissues were significantly higher than those in pericarcinomatous tissues(71.43% vs 14.29%, 68.73% vs 16.33%; P<0.05). The expressions of PIM2 and PFKFB3 were related to clinical stage, lymph node metastasis and degree of differentiation(P<0.05), but were not associated with age, gender, tumor diameter and pathological type(P>0.05). The results of multivariate Cox regression analysis showed that clinical stages Ⅲ and Ⅳ, lymph node metastasis, positive expression of PIM2 and positive expression of PFKFB3 were independent risk factors for death in the NSCLC patients(P<0.05). The 3-year overall survival rate of the 98 NSCLC patients was 54.08%. The survival prognosis of the patients with negative expressions of PIM2 and PFKFB3 was better than that of the patients with positive expressions of PIM2 and PFKFB3(log-rank test: χ²=5.777, P=0.016; χ²=5.422, P=0.020). Conclusion　Positive expressions of PIM2 and PFKFB3 are associated with the adverse clinicopathological features and poor prognosis of the patients.]]> 2025/7/4 11:15:38 1, HUANG Qinrong¹, YANG Jinping², HUANG Na³, YANG Li³]]> WEI Jiali¹, HUANG Qinrong¹, YANG Jinping², HUANG Na³, YANG Li³ 151true ［Abstract］　Objective　To observe the clinical effectiveness of laparoendoscopic single-site nipple-areola-sparing subcutaneous mastectomy(NSM) combined with prosthetic implant breast reconstruction in treatment of breast cancer. Methods　The clinical data of 101 patients with breast cancer who were admitted to the Tenth Affiliated Hospital, Southern Medical University(Dongguan People′s Hospital) from August 2020 to August 2023 were retrospectively analyzed. According to different surgical methods received by the patients, they were divided into observation group(receiving laparoendoscopic single-site NSM combined with prosthetic implant breast reconstruction, 39 cases) and control group(receiving traditional open surgery combined with prosthetic implant breast reconstruction, 62 cases). The perioperative indicators, serum tumor marker levels, prognosis and cosmetic outcome of the breast were compared between the two groups. Results　The operative duration, surgical incision length and length of stay in hospital in the observation group were shorter than those in the control group, and the number of incisions and intraoperative blood loss in the observation group were less than those in the control group, with statistically significant differences between the two groups(P<0.05). There was no statistically significant difference in the incidence of complications between the two groups(P>0.05). There were no statistically significant differences in the levels of serum tumor markers between the two groups before and after operation(P>0.05). As of October 2024, no local recurrence occurred in both groups. In the control group, 2 patients had distant tumor metastasis, including 1 case of supraclavicular lymph node metastasis and 1 case of bone metastasis. No distant tumor metastasis occurred in the observation group. There were no death patients in both groups during the follow-up period. The proportion of excellent and good Harris Scale scores in the observation group was higher than that in the control group, and the overall situation of cosmetic outcome of the breast in the observation group was better than that in the control group, with statistically significant difference between the two groups(P<0.05). Conclusion　Laparoendoscopic single-site NSM combined with prosthetic implant breast reconstruction is minimally invasive, with good prognosis and favorable cosmetic outcome of the breast after operation, and is recommended for clinical use in treatment of breast cancer.]]> 2025/7/4 11:15:38 1,2, DENG Runshu¹, YUAN Yongxian¹, DENG Dingmei¹, WEN Runyao¹, HE Guangning¹]]> YE Xian^1,2, DENG Runshu¹, YUAN Yongxian¹, DENG Dingmei¹, WEN Runyao¹, HE Guangning¹ 150true ［Abstract］　Objective　To analyze the clinical features of kidney disease pediatric patients complicated with posterior reversible encephalopathy syndrome(PRES). Methods　The clinical data of 8 kidney disease pediatric patients complicated with PRES who were admitted to the Affiliated Women′s and Children′s Hospital, School of Medicine, University of Electronic Science and Technology of China from August 2017 to August 2024 were retrospectively analyzed. The clinical manifestations, auxiliary examination results, treatment and prognosis of the pediatric patients were summarized. Results　Glucocorticoids had been used in 8 pediatric patients, and immunosuppressants had been used in 7 pediatric patients. The main clinical manifestations of the pediatric patients were hypertension, headache, dizziness, convulsions, consciousness disorders and visual disturbances. Cranial magnetic resonance imaging(MRI) showed that the lesions mainly involved the parieto-occipital cortex and subcortex(7 cases), and possibly involved the frontal lobe(5 cases), temporal lobe(2 cases), semioval center(2 cases), and bilateral ventricles(1 case). After symptomatic treatment, both the clinical manifestations and MRI examination results of the pediatric patients improved. As of December 2024, there were no relapses of PRES in the 8 pediatric patients during the follow-up period. Conclusion　The kidney disease pediatric patients can be complicated with PRES. The complicated PRES is associated with primary diseases, hypertension, and use of glucocorticoids and immunosuppressants to a certain extent in the kidney disease pediatric patients. Cranial MRI examination is helpful for early diagnosis. Timely and reasonable treatment can minimize the damage to the nervous system.]]> 2025/7/4 11:15:38 YANG Qin, FENG Shipin 149true ［Abstract］　Objective　To compare the clinical efficacy of modified subxiphoid and subcostal incision procedure and inflatable subxiphoid approach for thymic tumor resection. Methods　The clinical data of 80 patients with thymic tumors who were admitted to Baoding No.2 Central Hospital from June 2021 to February 2024 were retrospectively analyzed. According to different treatment methods, the patients were divided into control group(undergoing inflatable subxiphoid approach for thymic tumor resection, 38 cases) and observation group(undergoing modified subxiphoid and subcostal incision procedure for thymic tumor resection, 42 cases). The operation-related indicators, pulmonary function indicators, inflammatory indicators, postoperative Visual Analogue Scale(VAS) scores, the number of compressions of the analgesic pump, clinical efficacy and the occurrence of postoperative adverse reactions were compared between the two groups. Results　The operation time, time of postoperative indwelling catheter and length of stay in hospital in the observation group were shorter than those in the control group, and the intraoperative blood loss and postoperative drainage volume in the observation group were less than those in the control group, with statistically significant differences between the two groups(P<0.05). The levels of forced vital capacity(FVC) and forced expiratory volume in 1 second(FEV₁) after operation in the observation group were higher than those in the control group, and the levels of erythrocyte sedimentation rate(ESR), C-reactive protein(CRP), and interleukin-6(IL-6) after operation in the observation group were lower than those in the control group, with statistically significant differences between the two groups(P<0.05). At 4 hours and 24 hours after operation, the VAS scores in the observation group were lower than those in the control group, and the number of compressions of the analgesic pump after operation in the observation group was less than that in the control group, with statistically significant differences between the two groups(P<0.05). The total clinical effective rate of the observation group was significantly higher than that of the control group(90.48% vs 73.68%; χ²=3.869, P=0.048), and the incidence of postoperative adverse reactions in the observation group was significantly lower than that in the control group(7.14% vs 23.68%; χ²=4.281, P=0.038). Conclusion　Compared with the inflatable subxiphoid approach, the modified subxiphoid and subcostal incision procedure has less intraoperative blood loss, faster recovery for the patients, higher total clinical effective rate, and lower incidence of adverse reactions in resection of thymic tumors. The modified subxiphoid and subcostal incision procedure is worthy of clinical recommendation.]]> 2025/7/4 0:00:00 MA Xingang, YUAN Xin, WANG Yanbing, CHEN Xiaofeng, YAN Yingguang, WANG Xingang 148true ［Abstract］　Objective　To analyze the clinical characteristics of autoimmune encephalitis(AE) in children. Methods　A retrospective analysis was conducted on the clinical data of 36 pediatric patients with AE who were admitted to Weifang People′s Hospital from May 2020 to September 2024. Their clinical manifestations, auxiliary examination results, treatments and prognosis were summarized and analyzed. Results　Among the 36 patients, 20 patients were male(55.56%) and 16 patients were female(44.44%). The minimum age of the patient at disease onset was 1 year, the maximum age of the patient at disease onset was 13 years and 6 months, and the average age of the patient at disease onset was (7.21±3.88)years. There were 16 patients(44.44%) with acute onset(<1 week) and 20 patients(55.56%) with subacute onset(1 week to 3 months). Twenty-one patients(58.33%) had prodromal infection symptoms before disease onset. Epileptic seizures were the most common symptom［19 patients(52.78%)］. The cerebrospinal fluid(CSF) properties and transparency of 36 patients were basically normal. The number of nucleated cells in the CSF was increased(>5×10⁶/L) in 21 patients(58.33%), and the CSF chlorine was increased(>130 mmol/L) in 3 patients(8.33%), and abnormal CSF protein occurred in 14 patients(38.89%), and abnormal CSF glucose occurred in 7 patients(19.44%). No pathogens were found in the CSF specimens of all the patients. Among the 36 patients, anti-N-methyl-D-aspartate receptor(NMDAR) antibody was positive in 10 patients(27.78%), and anti-myelin oligodendrocyte glycoprotein(MOG) antibody was positive in 7 patients(19.44%), and anti-contactin-associated protein-like 2(CASPR2) antibody was positive in 6 cases(16.67%). Twenty-eight patients(77.78%) had electroencephalogram(EEG) abnormalities. Thirty-five patients underwent cerebral magnetic resonance imaging(MRI) examinations, among whom 14 patients(40.00%) showed abnormal manifestations. Among the patients who received immunotherapy, 68.75%(22/32) of them had a complete recovery without any neuropsychiatric sequelae. Conclusion　School age period is the peak period for the onset of AE in children. Its clinical manifestations mostly present with acute or subacute onset, often accompanied by a history of prodromal infection, and epileptic seizures are the most common symptom. The children with anti-NMDAR encephalitis account for the highest proportion of the children with AE. The abnormal rate of EEG in AE children is relatively high, but the abnormities lack specificity. Early standardized immunotherapy has a significant effect on most AE children.]]> 2025/7/4 0:00:00 1, WANG Qianyun², SHAO Xianli², ZHANG Fan¹, ZHAO Huafeng²]]> XU Li¹, WANG Qianyun², SHAO Xianli², ZHANG Fan¹, ZHAO Huafeng² 147true ［Abstract］　Objective　To analyze the effect of non-invasive prenatal testing(NIPT) on detection of sex chromosome aneuploidies(SCAs) and to consider the relevant ethical issues. Methods　The clinical data of 17 211 pregnant women who received genetic counseling and prenatal karyotype analysis in the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2005 to December 2023 were retrospectively analyzed. The changes in the detection rates of SCAs before and after implementation of NIPT, as well as the distribution of indications for SCAs in prenatal diagnosis were analyzed. The factors influencing the detection of 47,XXX were explored. The pregnancy options of pregnant women with different types of SCAs were summarized. Results　Before implementation of NIPT(before 2015), the detection rate of SCAs was 0.35%(20/5 680), and the detection rate of 47,XXX was 0.07%(4/5 680). After implementation of NIPT, the detection rate of SCAs rose to 1.35%(156/11 531), and the detection rate of 47,XXX rose to 0.24%(28/11 531). The detection rates of 47,XXX from 2005 to 2023 were positively correlated with the total detection rate of SCAs(r=0.812, P<0.001), the age of pregnant women(r=0.719, P<0.001), and the detection quantity of NIPT(r=0.812, P<0.001). Among the 152 pregnant women with prenatal diagnosis results of SCAs, high risk of NIPT was the main indication, accounting for 40.79%(62/152), followed by simple advanced age, accounting for 15.13%(23/152). A total of 134 pregnant women with SCAs were followed up, among whom 71 pregnant women(52.99%) chose the termination of pregnancy. Among the 71 pregnant women choosing the termination of pregnancy, the rate of termination of pregnancy in the pregnant women whose fetuses carried 47,XXX was the highest(70.83%, 17/24), followed by that in the pregnant women whose fetuses carried 45,X(66.67%, 22/33). Conclusion　The implementation of NIPT significantly increases the detection rate of SCAs, but its application in screening and diagnosis involves complex ethical issues. Clinical genetic counseling service should be actively carried out to reduce unnecessary terminations of pregnancy due to the patients′ cognitive biases.]]> 2025/7/4 11:15:38 ZHANG Peng, MO Weiying, MENG Minghui, ZHANG Hongyan 146true ［Abstract］　Objective　To explore the effect of anti-tumor natural product Garcinone E on autophagy function of ovarian cancer cells and its mechanism. Methods　Ovarian cancer cells HeyA8 and SKOV3 were selected for experiments. Ovarian cancer cells were intervened with different concentrations of Garcinone E. Cell viability was detected by CCK-8 assay, and autophagic vesicle formation was detected by monodansylcadaverine(MDC) staining assay. The HeyA8 cell line and SKOV3 cell line stably expressing stubRFP-sensGFP-LC3 lentivirus were constructed to detect the changes in cellular autophagic flux. Co-localization of lysosomes with sensGFP-LC3 in ovarian cancer cells was detected by using lysosomal staining assay. Western blot assay was applied to detect the expression levels of cell cycle-related proteins p21, cyclin-dependent kinase 4(CDK4), cyclin-dependent kinase 7(CDK7), protein kinase B(Akt), microtubule-associated protein 1-light chain 3B(LC3B) and autophagy-related protein p62 in ovarian cancer cells. Results　The viability of ovarian cancer cells was significantly decreased(P<0. 05) after intervention with Garcinone E. The half maximal inhibitory concentration(IC₅₀) of Garcinone E on HeyA8 cells and SKOV3 cells were 5.93 μmol/L and 8.14 μmol/L, respectively. The MDC staining assay revealed an increase in the number of autophagosomes in the ovarian cancer cells after intervention with Garcinone E. Fluorescence microscopy revealed that Garcinone E blocked the autophagic flux in HeyA8 cells and SKOV3 cells stably transfected with stubRFP-sensGFP-LC3 lentivirus. The results of lysosomal staining assay showed that Garcinone E blocked the binding of autophagosomes to lysosomes. The results of Western blot assay showed that the intervention with Garcinone E up-regulated the protein expression levels of LC3B, p62 and p21 in ovarian cancer cells(P<0. 05) and down-regulated the protein expression levels of Akt, CDK4 and CDK7(P<0. 05). Conclusion　Garcinone E can inhibit the autophagic flux of ovarian cancer cells and block the cell cycle of ovarian cancer cells, which has an anti-ovarian cancer effect and the potential to serve as a lead compound in the development of chemotherapeutic drugs for ovarian cancer.]]> 2025/6/5 0:00:00 1, LEI Shunmei¹, WANG Chunlai¹, DAI Yiping¹, WEI Dan¹, YIN Fuqiang², LIU Xia¹]]> SUN Xuemei¹, LEI Shunmei¹, WANG Chunlai¹, DAI Yiping¹, WEI Dan¹, YIN Fuqiang², LIU Xia¹ 145true ［Abstract］　Objective　To observe the therapeutic effect of transurethral 1 470-nm wavelength laser prostatectomy with small-piece removal without morcellation in a tissue pulverizer for treatment of high-risk benign prostatic hyperplasia(BPH). Methods　The clinical data of 100 patients with high-risk BPH who were admitted to Guangdong Provincial People′s Hospital from July 2023 to July 2024 were retrospectively analyzed. The patients were divided into observation group(receiving transurethral 1 470-nm wavelength laser prostatectomy with small-piece removal without morcellation in a tissue pulverizer, 50 cases) and control group(receiving transurethral 1 470-nm wavelength laser enucleation of the prostate in collocation with a tissue pulverizer, 50 cases) according to different surgical methods. The surgical-related indicators, postoperative recovery, International Prostate Symptom Scores(IPSS) and quality of life(QOL) scores before surgery and 3 months after surgery, and the incidence of complications within 3 months after surgery were compared between the two groups. Results　The operation time in the observation group was shorter than that in the control group, and the amount of intraoperative flushing fluid used in the observation group was less than that used in the control group, with statistically significant differences between the two groups(P<0.05). However, there was no statistically significant difference in the decrease in hemoglobin levels between the two groups on the first day after surgery(P>0.05). There was no statistically significant difference in the postoperative flushing time and indwelling time of the urinary catheter between the two groups(P>0.05). The IPSS and QOL scores in both groups 3 months after surgery were lower than those before surgery, and the differences were statistically significant(P<0.05). However, there were no significant differences in IPSS and QOL scores between the two groups before surgery and 3 months after surgery(P>0.05). No complications occurred in the observation group. One case of urethral stricture occurred in the control group(2.00%). There was no significant difference in the incidence of complications between the two groups(χ²=0.126, P=0.277). Conclusion　The transurethral 1 470-nm wavelength laser prostatectomy with small-piece removal without morcellation in a tissue pulverizer is effective and safe in treatment of high-risk BPH, and can simplify the surgical process and effectively shorten the surgical time.]]> 2025/6/5 0:00:00 1, TAO Yingxu¹, DENG Jiwang¹, LI Guobang¹, ZHONG Ruijuan², WEN Xingqiao¹]]> LI Xiangping¹, TAO Yingxu¹, DENG Jiwang¹, LI Guobang¹, ZHONG Ruijuan², WEN Xingqiao¹ 144true ［Abstract］　Objective　To explore the correlation between vaginal dysbacteriosis and intrauterine adhesion(IUA). Methods　Thirty patients with IUA who were treated in the Third Xiangya Hospital of Central South University from January 1, 2022 to May 31, 2022 were recruited as IUA group. Other 30 healthy women who underwent hysteroscopy at the same time to exclude IUA were selected as control group. A sample of secretions was collected at the midvagina using a sterile swab. The variable region of the 16S rRNA gene was amplified through polymerase chain reaction, and high-throughput sequencing was performed on the MiSeq Illumina sequencing platform. The sequencing data were processed through bioinformatics analysis method to analyze the bacterial diversity. Results　The results of bacterial diversity analysis showed that the bacterial diversity in the IUA group was lower than that in the control group. The grade-abundance distribution curve suggested that the bacterial abundance in the midvagina was higher and the species were more evenly distributed in the control group. The Alpha diversity analysis results showed that the Chao index, ACE index, pedigree diversity index, Shannon′s index and the number of observed species in the IUA group were significantly lower than those in the control group(P<0.05), while the Simpson′s index in the IUA group was significantly higher than that in control group(P<0.05). The relative abundance of Firmicutes in the IUA group was significantly higher than that in the control group(P<0.05), while the relative abundances of Actinomyces, Bacteroides, Proteus, Fusobacteria, Planctomycetes, verrucous microorganisms and G.oxydans in the IUA group were significantly lower than those in the control group(P<0.05). The relative abundances of Lactobacillus prophages Lj928, Klebsiella pneumoniae and Roseburia inulinivorans in the IUA group were significantly higher than those in the control group(P<0.05), while the relative abundances of Atopobium vaginae, Bifidobacteria, Pseudomonas, Prevotella, Aeromonas veronii, Shewanella xiamenensis, Bacteroides melaninogenicus, Porphyromonas and Phascolarctobacteriumin the IUA group were significantly lower than those in the control group(P<0.05). Conclusion　The richness and diversity of vaginal flora decrease significantly in IUA patients, which may be one of the mechanisms of IUA. Improving vaginal dysbacteriosis may contribute to the prevention and treatment of IUA.]]> 2025/6/5 10:27:59 1,2, FAN Jieru¹, HUANG Huan¹, ZHAO Xingping¹, SHU Changfa¹, WANG Haiyan¹]]> SHEN Rongjiong^1,2, FAN Jieru¹, HUANG Huan¹, ZHAO Xingping¹, SHU Changfa¹, WANG Haiyan¹ 143true ［Abstract］　Objective　To explore the efficacy of staged laparoscopic Fowler-Stephens orchidopexy via direct inguinal triangle approach for high undescended testis. Methods　Two hundred pediatric patients with high undescended testes who were treated in the Department of Pediatric Surgery of Linyi Central Hospital from February 2020 to February 2024 were recruited. The pediatric patients were divided into control group(receiving laparoscopic testicular descent and fixation via direct inguinal triangle approach) and observation group(receiving staged laparoscopic Fowler-Stephens orchidopexy via direct inguinal triangle approach) by random number table method, with 100 cases in each group. During the treatment process, 11 cases dropped out in the control group, and 89 cases were eventually included. During the treatment process, 2 cases dropped out in the observation group, and 98 cases were eventually included. Ultimately, 75 pairs of the pediatric patients were successfully matched after conducting match at a ratio of 1∶1 through propensity score matching(PSM). The changes in testicular volume, sex hormone levels and testicular function were compared between the two groups before surgery and 6 months after surgery. The therapeutic effects and the occurrence of complications were compared between the two groups. Results　Compared with that before operation, there was no significant change in testicular volume between the two groups after operation(P>0.05), and there were no statistically significant differences in testicular volume and clinical efficacy between the two groups after operation(P>0.05). Compared with those before operation, the levels of follicle-stimulating hormone(FSH) and estradiol(E₂) decreased and the levels of testosterone(T), anti-Müllerian hormone(AMH), and inhibin B(INHB) increased in both groups after operation, with statistically significant differences(P<0.05), but there were no statistically significant differences between the two groups after operation(P>0.05). The total occurrence rates of complications in the observation group and the control group were 8.00% and 12.00%, respectively, with no statistically significant difference between the two groups(P>0.05). Conclusion　The staged laparoscopic Fowler-Stephens orchidopexy via direct inguinal triangle approach is safe and effective in treatment of high undescended testis.]]> 2025/6/5 0:00:00 1,2, WANG Qian³, YANG Binyi¹, LU Hongting¹]]> CAO Qingwei^1,2, WANG Qian³, YANG Binyi¹, LU Hongting¹ 142true ［Abstract］　Objective　To compare the effects of high-flux hemodialysis(HFHD), hemodiafiltration(HDF) and continuous hemodialysis(CHD) on clearance of mid-molecule substance and macromolecular substance related to renal function in patients with renal failure. Methods　A total of 100 patients with renal failure who were admitted to the 983rd Hospital of Joint Logistic Support Force of PLA from January 2021 to December 2022 and received regular hemodialysis treatment were recruited. These patients′ mid-molecule substance and macromolecular substance related to renal function remained at sustained high levels. After fully informing the patients of their conditions, they were divided into CHD group(33 cases), HDF group(33 cases) and HFHD group(34 cases) according to their treatment wishes. The levels of small-molecule substance related to renal function(creatinine, urea nitrogen, phosphorus), mid-molecule substance and macromolecular substance related to renal function(albumin, β₂-microglobulin, C-reactive protein), and oxidative stress indicators［malondialdehyde(MDA), superoxide dismutase(SOD), catalase, thioredoxin］ were compared among the three groups before adjusting the dialysis modes and 6 months after adjusting the dialysis modes. The occurrence of complications in the two groups were recorded. Results　After adjusting the dialysis modes, the levels of creatinine, urea nitrogen and phosphorus in the three groups were significantly lower than those before adjusting the dialysis modes(P<0.05), but there were no statistically significant differences in the levels of creatinine, urea nitrogen and phosphorus among the three groups after adjusting the dialysis modes(P>0.05). After adjusting the dialysis modes, the levels of albumin, SOD, catalase and thioredoxin in the three groups were significantly increased compared with those before adjusting the dialysis modes(P<0.05), and the levels of β₂-microglobulin, C-reactive protein and MDA in the three groups were significantly decreased compared with those before adjusting the dialysis modes(P<0.05). After adjusting the dialysis modes, the levels of β₂-microglobulin, C-reactive protein and MDA in the HDF group were significantly lower than those in the CHD group and the HFHD group(P<0.05). After adjusting the dialysis modes, the levels of SOD, catalase and thioredoxin in the HDF group were significantly higher than those in the CHD group and the HFHD group(P<0.05). After adjusting the dialysis modes, there was no statistically significant difference in the level of albumin among the three groups(P>0.05). The total incidence of complications in the CHD group, the HDF group and the HFHD group was 18.18%, 12.12% and 14.71% respectively, and there was no statistically significant difference in the total incidence of complications among the three groups(χ²=0.478, P=0.788). Conclusion　Among the three dialysis modes of HFHD, HDF and CHD, HDF is the most effective in clearing the mid-molecule substance and macromolecular substance related to renal function and is the most effective in improving the oxidative stress state, and has good safety.]]> 2025/6/5 10:27:59 1, LIU Ping²]]> Yawei¹, LIU Ping² 141true ［Abstract］　Objective　To observe the effect of pulsed radiofrequency(PRF) combined with intravenous drip of lidocaine in treatment of acute herpetic neuralgia(AHN). Methods　A total of 60 patients with AHN who were admitted to Department of Interventional Vascular Pain Management, the Second People′s Hospital of Hefei Affiliated to Wannan Medical College from April 2023 to June 2024 were recruited. The patients were divided into observation group and control group by using random number table method, with 30 cases in each group. The observation group received PRF combined with intravenous drip of lidocaine(once a day for 7 days), while the control group received PRF combined with intravenous drip of normal saline treatment. The patients were given tramadol sustained-release tablets orally, 100 mg each time, once every 12 hours. If the patients′ pain levels were reduced to mild pain, the dose of oral tramadol sustained-release tablets was changed to 50 mg each time, once every 12 hours. When the patients′ Visual Analogue Scale(VAS) scores were less than 3 points, the oral tramadol sustained-release tablets were discontinued. The VAS scores, Hospital Anxiety and Depression Scale(HADS) scores［including Hospital Anxiety Scale(HA) scores and Hospital Depression Scale(HD) scores］ and Pittsburgh Sleep Quality Index(PSQI) scores were compared between the two groups at admission(T₀), 3 days after surgery(T₁), 1 week after surgery(T₂), 1 month after surgery(T₃) and 3 months after surgery(T₄). The dosage of tramadol during hospitalization(T₀-T₂ time points), postoperative adverse reactions and the occurrence of post-herpetic neuralgia(PHN) were compared between the two groups. Results　The postoperative VAS scores and PSQI scores showed a downward trend in the two groups(P<0.05). The VAS scores of the observation group were significantly lower than those of the control group at the time points of T₃ and T₄(P<0.05), and the PSQI scores of the observation group were lower than those of the control group at the time points of T₁, T₂, T₃ and T₄(P<0.05). The HA scores and HD scores showed a downward trend in the two groups(P<0.05), but there were no significant differences in the HA scores and HD scores between the two groups at each time point(P>0.05). The dosage of tramadol in the observation group was significantly lower than that in the control group during hospitalization(P<0.05). There was no significant difference in the cumulative incidence of PHN between the two groups at the time point of T₃(P>0.05), while the cumulative incidence of PHN in the observation group was significantly lower than that in the control group at the time point of T₄(P<0.05). The total incidence of adverse reactions in the observation group was higher than that in the control group, but the difference was not statistically significant(26.67% vs 10.00%; χ²=2.783, P=0.095). Conclusion　PRF combined with intravenous drip of lidocaine is safe and effective in treatment of AHN, and can reduce the risk for the occurrence of PHN.]]> 2025/6/5 10:27:59 1,2, TANG Ming¹, WANG Yiwen¹, QIAN Chenhuiyu³, PAN Shengquan¹, YIN Shiwu¹]]> CHANG Xindong^1,2, TANG Ming¹, WANG Yiwen¹, QIAN Chenhuiyu³, PAN Shengquan¹, YIN Shiwu¹ 140true ［Abstract］　Objective　To analyze the effects of early postoperative intra-articular injection of platelet-rich plasma(PRP) into the knee joint on inflammatory cytokine levels and knee joint function in patients with Schatzker type Ⅴ and Schatzker type Ⅵ tibial plateau fractures. Methods　A total of 40 patients with Schatzker type Ⅴ and Schatzker type Ⅵ tibial plateau fractures who were admitted to the First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology from January 2023 to December 2024 were recruited. The patients were divided into observation group and control group by using random number table method, with 20 cases in each group. All the patients underwent open reduction and internal fixation with plate and screws for tibial plateau fractures. In the observation group, 10 mL of PRP was injected into the knee joint at the 1st week, 3rd week and 5th week after surgery. In the control group, 10 mL of normal saline was injected into the knee joint at the 1st week, 3rd week and 5th week after surgery. The knee joint fluid was collected from the patients at the 3rd week(before injecting PRP/normal saline), 5th week(before injecting PRP/normal saline), and 7th week after surgery to detect the levels of interleukin-1β(IL-1β), tumor necrosis factor-α(TNF-α), and interleukin-6(IL-6). The knee joint function was assessed by using the Hospital for Special Surgery Knee Score(HSS) at the 3rd month after surgery. Results　The levels of IL-6 and TNF-α in the observation group were significantly lower than those in the control group at the 3rd week, 5th week, and 7th week after surgery(P<0.05). Compared with those at the 3rd week after surgery, the levels of IL-1β and TNF-α in the observation group were significantly reduced at the 5th week and 7th week after surgery(P<0.05). Compared with those at the 3rd week after surgery, the levels of IL-6 in the observation group were significantly reduced at the 7th week after surgery(P<0.05). The observation group had higher scores in six aspects including pain, function, range of motion, muscle strength, flexion deformity and stability, and higher total HSS scores than the control group, with statistically significant differences between the two groups(P<0.05). Conclusion　Early postoperative intra-articular injection of PRP into the knee joint in patients with Schatzker type Ⅴ and Schatzker type Ⅵ tibial plateau fractures can reduce the inflammatory cytokine levels in the knee joint fluid and improve knee joint function.]]> 2025/6/5 0:00:00 1, ZHANG Wenlong², WANG Zhifeng¹, ZHANG Zhiyan³]]> WANG Jizhu¹, ZHANG Wenlong², WANG Zhifeng¹, ZHANG Zhiyan³ 139true ［Abstract］　Objective　To explore the clinical value of fecal calprotectin(FCP) in assisting diagnosis of upper gastrointestinal inflammatory diseases in children. Methods　The clinical data of 165 pediatric patients who visited their doctors in Shanghai Children′s Medical Center, Shanghai Jiao Tong University School of Medicine and Department of Gastroenterology, Guizhou Branch of Shanghai Children′s Medical Center, Shanghai Jiao Tong University School of Medicine from June 2023 to June 2024 were retrospectively analyzed. The pediatric patients were divided into upper gastrointestinal tract inflammatory disease group(66 cases) and functional gastrointestinal disease group(99 cases). Fecal samples were collected for detection of FCP, and the differences between groups were compared. Receiver operating characteristic(ROC) curve was used to evaluate the efficacy of FCP in diagnosing upper gastrointestinal tract inflammatory disease in the children. Results　The level of FCP in the upper gastrointestinal tract inflammatory disease group［78.38(16.64,175.70)μg/g］ was higher than that in the functional gastrointestinal disease group［14.90(5.00, 33.76)μg/g］, with a statistically significant difference between the two groups(Z=5.230, P<0.001). FCP could assist diagnosis of upper gastrointestinal tract inflammatory disease in the children［AUC(95%CI)=0.74(0.66-0.82), P<0.001］ with the optimum cut-off value of 96.32 μg/g, and the diagnostic sensitivity and specificity were 50.00% and 91.90%, respectively. Conclusion　FCP, as a non-invasive indicator, has clinical value in the auxiliary diagnosis of upper gastrointestinal tract inflammatory disease in children.]]> 2025/6/5 10:27:59 1,2, LIU Lianhua², WU Ying^3,4, TENG Teng^5,6, DENG Zhaohui¹]]> WANG Wenyu^1,2, LIU Lianhua², WU Ying^3,4, TENG Teng^5,6, DENG Zhaohui¹ 138true ［Abstract］　Objective　To analyze the clinicopathological features of 5 cases of aggressive natural killer(NK)-cell leukemia(ANKL). Methods　The clinicopathological data of 5 patients with ANKL who were admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region from October 2015 to July 2021 were retrospectively analyzed. Immunohistochemical staining was used to detect the expressions of cytoplasmic CD3, CD45RO, CD56 and granzyme B, and T-cell intracellular antigen-1(TIA-1), CD4, CD5, CD8, CD20, myeloperoxidase(MPO), E-cadherin and Ki-67. The expression of Epstein-Barr virus(EBV)-encoded small RNA(EBER) was detected by using in situ hybridization method. Results　Among the 5 patients with ANKL, 4 patients were male and 1 patient was female, aged 16-85 years. The patients presented with high fever(5/5), accompanied by hepatosplenomegaly(3/5), elevated lactate dehydrogenase(5/5), peripheral pancytopenia(5/5) and coagulation dysfunction(5/5). The results of bone marrow biopsy revealed that the tumor cells showed interstitial or patchy infiltration, with medium-sized cell volume and pleomorphic cells, and showed signs of apoptosis and mitosis in the tissue hematoxylin-eosin(HE) slices. The results of immunohistochemical staining showed that cytoplasmic CD3, CD45RO, CD56, granzyme B and TIA-1 were positive, while CD4, CD5, CD8, CD20, MPO and E-cadherin were negative. The Ki-67 proliferation index was approximately 80%. The detection results of in situ hybridization showed that the tumor cells were EBER positive. One patient underwent chemotherapy and survived for 3 months. Four patients gave up treatment and had a survival period of 1-3 months. Conclusion　ANKL is a rare systemic NK cell tumor, which is closely related to EBV infection. The disease progresses rapidly and needs to be differentiated from other mature T- and NK-cell lymphomas/leukemia.]]> 2025/6/5 10:27:59 1, HUANG Yongta¹, LUO Yu², GUO Wenwen¹, MO Xianglan¹]]> LIU Xia¹, HUANG Yongta¹, LUO Yu², GUO Wenwen¹, MO Xianglan¹ 137true ［Abstract］　Objective　To analyze the etiology and antibiotic resistance profile of diarrheagenic Escherichia coli(DEC) in children with diarrhea in a hospital in Guilin City. Methods　A total of 793 fresh fecal specimens from diarrhea children aged 0-14 years who were treated in the Children′s Outpatient Department of Guilin Maternal and Child Health Hospital from January 2022 to December 2023 were collected for isolation and culture of Escherichia coli. The virulence genes of the bacterial strains were detected by using polymerase chain reaction(PCR) to determine different pathogenic types of DEC. The sensitivity of the bacterial strains to 19 kinds of antibiotics in 10 categories was determined by using the broth microdilution method. Results　DEC was detected in 49 fecal specimens of the 793 fecal specimens(6.18%). Among the different pathogenic types of DEC, enteroaggregative Escherichia coli(EAEC) had the highest proportion(24 cases, 48.98%), followed by enteropathogenic Escherichia coli(EPEC)(23 cases, 46.94%). One case of enterohemorrhagic Escherichia coli or Shiga toxin-producing Escherichia coli(EHEC/STEC) carrying stx2f gene and one case of enterotoxigenic Escherichia coli(ETEC) carrying both stIa and stIb genes were detected. No enteroinvasive Escherichia coli(EIEC) was detected. The results of drug sensitivity test of 49 cases of DEC showed that the drug with the highest resistance rate was ampicillin(59.18%), followed by cefazolin(36.73%). All the strains were sensitive to meropenem, tigecycline, amikacin and colistin and 40.82% of the strains were resistant to more than three categories of the antibiotics. Conclusion　DEC is one of the main pathogenic bacteria causing diarrhea in children in Guilin City, and among the different pathogenic types of DEC, EAEC and EPEC are predominant. The drug resistance rates of the strains are relatively high, and the rational use of antibiotics should be emphasized in clinical practice.]]> 2025/6/5 10:27:59 1, LIU Zhibing², YE Yanzhen¹, LIANG Yong², MA Li¹, TANG Guorong²]]> QIN Wenzhen¹, LIU Zhibing², YE Yanzhen¹, LIANG Yong², MA Li¹, TANG Guorong² 136true ［Abstract］　Objective　To explore the mechanism of Ginkgo biloba leaf extract EGb761 improving sensitivity of colorectal cancer(CRC) cells to cisplatin(DDP). Methods　Human CRC cells RKO were selected for experiment, and control group, EGb761 group, DDP group and EGb761+DDP group were set up according to different intervention methods. The cholecystokinin-8(CCK-8) method was used to detect the effects of EGb761 and DDP on the viability of RKO cells. Real-time fluorescent quantitative polymerase chain reaction(RT-qPCR) and Western blot experiments were used to detect the expressions of sphingosine kinase 1(SphK1) and apoptosis-related factor cysteinyl aspartate-specific proteinase 3(Caspase 3), and B-cell lymphoma 2(BCL2). Flow cytometry experiment was used to detect the apoptosis level of RKO cells. The RKO cell lines with low expression of SphK1 were constructed through lentiviral transfection, and the effects of knocking down SphK1 and EGb761 intervention on the expression levels of apoptosis-related factors were analyzed. The main active ingredients of Ginkgo biloba leaves were searched from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP), and their abilities to bind to SphK1 were explored through molecular docking. Results　The results of CCK-8 experiment showed that EGb761 could inhibit the proliferation of RKO cells and enhance the sensitivity of RKO cells to DDP. The results of RT-qPCR experiment and Western blot experiment showed that compared with those in the control group, the mRNA and protein expression levels of Caspase 3 in the EGb761 group, the DDP group and the EGb761+DDP group were significantly increased(P<0.05), and the mRNA and protein expression levels of Caspase 3 in the EGb761+DDP group were higher than those in the EGb761 group and the DDP group(P<0.05). Compared with those in the control group, the mRNA and protein expression levels of BCL2 in the EGb761 group, the DDP group and the EGb761+DDP group were significantly decreased(P<0.05), and the mRNA and protein expression levels of BCL2 in the EGb761+DDP group were lower than those in the EGb761 group and the DDP group(P<0.05). The results of flow cytometry experiment showed that compared with the control group, the apoptosis rates in the EGb761 group, the DDP group and the EGb761+DDP group were significantly increased(P<0.05), and the apoptosis rates in the EGb761+DDP group were higher than those in the EGb761 group and the DDP group(P<0.05). EGb761 could inhibit the expression of SphK1 protein in the RKO cells, and the inhibitory effect was dose-dependent(P<0.05). The RKO cell lines with low expression of SphK1 were successfully constructed by lentiviral transfection method. The experimental results showed that knocking down SphK1 could significantly enhance the sensitivity of RKO cells to DDP, while the actions of EGb761 on enhancing the sensitivity of RKO cells to DDP and promoting apoptosis depended on the inhibition of SphK1. The results of molecular docking showed that the main active ingredients of bilobalide, ranunculin, sesamin, (+)-catechin, (-)-catechin and isorhamnetin in Ginkgo biloba leaves had strong abilities to bind to SphK1. Conclusion　Ginkgo biloba leaf extract EGb761 promotes apoptosis by inhibiting SphK1 expression, thereby enhancing the sensitivity of CRC cells to DDP.]]> 2025/4/30 0:00:00 NING Qiting, CHEN Xingmei, HUANG Shanpei, ZHU Liye, QIU Xinze, LIU Shiquan 135true ［Abstract］　Objective　To explore the effects of Garcinone C on lysosomal damage of nasopharyngeal carcinoma(NPC) cells and their related mechanisms. Methods　NPC cells HONE1 and S18 were selected for experiment, and Garcinone C was used as intervention treatment. CCK-8 assay was used to detect cell proliferation viability, and the phosphate ester method was used to detect acid phosphatase activity. The expression levels of lysosomal-associated membrane protein 1(LAMP1), galectin-3(Gal-3) and cathepsin D(CTSD) were detected by using immunofluorescence assay, and their localizations were observed. The expression levels of CTSD, LAMP1, Gal-3, Ras-related protein Rab-7a(Rab7a) and vesicle-associated membrane protein 8(VAMP8) were detected by using Western blot. Results　Garcinone C could inhibit the viability of NPC cells and the activity of acid phosphatase(P<0.05), and promote an increase in the expression levels of LAMP1, Gal-3 and CTSD in the cytoplasm(P<0.05). After intervention with Garcinone C, the expression levels of CTSD, LAMP1, Gal-3 and Rab7a proteins in NPC cells were significantly increased(P<0.05), while the expression level of VAMP8 protein was significantly decreased(P<0.05). These regulatory effects were dose-dependent. Conclusion　Garcinone C may play a therapeutic role by damaging lysosomes of NPC cells. Garcinone C is expected to be a candidate lead compound in the development of chemotherapy drugs for NPC.]]> 2025/4/30 0:00:00 1, YIN Fuqiang², WANG Chunlai¹, SUN Xuemei¹, ZHANG Jinyan³, LIU Xia¹]]> DAI Yiping¹, YIN Fuqiang², WANG Chunlai¹, SUN Xuemei¹, ZHANG Jinyan³, LIU Xia¹ 134true ［Abstract］　Objective　To analyze the expressions of miR-129-5p and chromobox homolog 4(CBX4) in hepatocellular carcinoma(HCC) tissues and their clinical significance. Methods　The clinical data of 120 HCC patients were collected from the Affiliated Hospital of Inner Mongolia Medical University between August 2018 and August 2021. Bioinformatics method was used to analyze the CBX4 protein level in HCC tissues and normal tissues and the targeted regulatory relationship between miR-129-5p and CBX4. The cancerous tissues and their para-cancerous tissues(≥5 cm from the cancerous tissues) were collected from the patients. Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR) was used to detect the levels of miR-129-5p and CBX4 mRNA in the tissues. According to the average level of miR-129-5p in the cancerous tissues, the patients were divided into low miR-129-5p expression group and high miR-129-5p expression group. The expression of CBX4 protein was detected by using immunohistochemical staining. According to the expressions of CBX4 protein in the cancerous tissues, the patients were divided into CBX4 positive expression group and CBX4 negative expression group. The survival of the patients during the follow-up period of 3 years was recorded. Pearson correlation was used to analyze the correlation between miR-129-5p and CBX4 mRNA level in the cancerous tissues. Kaplan-Meier method was used to plot the survival curves, and log-rank test was used for comparison between groups. Cox regression was used to analyze the prognostic factors of the HCC patients. Results　The results of bioinformatics analysis showed that the level of CBX4 protein in the HCC tissues was significantly higher than that in the normal tissues(P<0.05), and there was a targeted regulatory relationship between miR-129-5p and CBX4 protein. The positive expression rate of CBX4 protein in the HCC patients′cancerous tissues was significantly higher than that in the HCC patients′ para-cancerous tissues［65.00%(78/120)vs 24.17%(29/120), χ²=40.492, P<0.05)］. The level of miR-129-5p in the cancerous tissues was lower than that in their para-cancerous tissues, while the level of CBX4 mRNA in the cancerous tissues was higher than that in their para-cancerous tissues, with statistically significant differences between the cancerous tissues and their para-cancerous tissues(P<0.05). There was a negative correlation between miR-129-5p level and CBX4 mRNA level in the cancerous tissues(r=-0.676, P<0.05). The proportion of the patients with clinical stages Ⅲ-Ⅳ, low tumor differentiation and vascular invasion in the low miR-129-5p expression group(<0.72) was significantly higher than that in the high miR-129-5p expression group(≥0.72)(P<0.05). The proportion of the patients with clinical stages Ⅲ-Ⅳ, low tumor differentiation and vascular invasion in the CBX4 positive expression group was significantly higher than that in the CBX4 negative expression group(P<0.05). The survival prognosis of the high miR-129-5p expression group was better than that of the low miR-129-5p expression group(log-rank test: χ²=19.048, P<0.001). The survival prognosis of the CBX4 negative expression group was better than that of the CBX4 positive expression group(log-rank test: χ²=11.977, P=0.001). The results of multivariate Cox regression analysis showed that high expression of miR-129-5p was an independent protective factor for the patients′ survival(P<0.05), while positive expression of CBX4 and clinical stages Ⅲ-Ⅳ were independent risk factors for the patients′ death(P<0.05). Conclusion　The level of miR-129-5p decreases and the level of CBX4 increases in HCC tissues, which are related to clinical stage, degree of tumor differentiation, vascular invasion and survival prognosis.]]> 2025/4/30 0:00:00 1, QI Qige¹, WANG Zexin²]]> JI Xiaolei¹, QI Qige¹, WANG Zexin² 133true ［Abstract］　Objective　To observe the application effect of implanting prosthesis by referring to transverse acetabular ligament positioning method in total hip arthroplasty(THA). Methods　The clinical data of 126 patients who underwent THA in Huangshi Central Hospital from January 2022 to October 2023 were retrospectively analyzed. According to the intraoperative acetabular prosthesis positioning methods, the patients were divided into observation group(using the refferring to transverse acetabular ligament positioning method, 64 cases) and control group(using the freehand visual inspection positioning method with a positioning rod, 62 cases). The operation time, intraoperative blood loss, hip function(Harris score), deviation of hip rotation center and the occurrence of complications were compared between the two groups. Results　The operation time of the observation group was shorter than that of the control group, and the intraoperative blood loss of the observation group was less than that of the control group, with statistically significant differences between the two groups(P<0.05). The vertical deviation of the hip rotation center in the observation group was significantly smaller than that in the control group(P<0.05), and there was no significant difference in the horizontal deviation between the two groups(P>0.05). The good reconstruction rate of the hip rotation center in the observation group was higher than that in the control group, and the difference was statistically significant(P<0.05). There were no significant differences in Harris scores between the two groups before operation(P>0.05), and the Harris scores in both groups showed an increasing trend after operation(P<0.05). At 1 month and 6 months after operation, the Harris scores of the observation group were significantly higher than those of the control group(P<0.05). The total incidence of postoperative complications in the observation group was lower than that in the control group, and there was statistically significant difference between the two groups(4.69% vs 16.13%; χ²=4.496, P=0.034). Conclusion　Implanting the prosthesis by referring to the transverse acetabular ligament positioning method in THA is helpful for reducing the deviation of hip rotation center after reconstruction and is beneficial to rehabilitation of the patients′ hip joint function.]]> 2025/4/30 0:00:00 1, SUN Farui², LI Bo², FENG Shaosheng¹, YE Hengyi¹, CHENG Gege¹]]> HUANG Chunyi¹, SUN Farui², LI Bo², FENG Shaosheng¹, YE Hengyi¹, CHENG Gege¹ 132true ［Abstract］　Objective　To observe the application effectiveness of musculoskeletal ultrasound-guided injection of platelet-rich plasma(PRP) in rehabilitation treatment of lateral epicondylitis(LE) patients after surgery. Methods　A total of 106 patients with LE who underwent arthroscopic-assisted radiofrequency ablation in the Second Hospital of Tangshan from March 2022 to March 2023 were recruited. The patients were divided into control group(53 cases) and observation group(53 cases) by using random number table method. After surgery, the control group received routine rehabilitation training, while the observation group received the same routine rehabilitation traing in combination with musculoskeletal ultrasound-guided injection of PRP. Both of the two groups were treated for 4 consecutive weeks. The pain intensity, levels of serum inflammatory factors, elbow joint function and upper limb function, pain-free grip strength(PFG) and activities of daily living(ADL) were compared between the two groups before rehabilitation treatment, at the 2nd week of rehabilitation treatment and at the 4th week of rehabilitation treatment. The excellent and good rate of elbow joint function recovery was compared between the two groups at the 6th month after surgery. The occurrence of complications during the postoperative rehabilitation treatment period was recorded in the two groups. Results　The Numeric Rating Scale(NRS) scores for assessing pain under forearm external rotation, pain under forearm internal rotation and resting pain in both groups at the 2nd week of rehabilitation treatment and at the 4th week of rehabilitation treatment were significantly lower than those before rehabilitation treatment(P<0.05), and the scores in the observation group were significantly lower than those in the control group after rehabilitation treatment(P<0.05). The levels of serum C-reactive protein(CRP), interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) in both groups at the 2nd week of rehabilitation treatment and at the 4th week of rehabilitation treatment were significantly lower than those before rehabilitation treatment(P<0.05), and the levels in the observation group were significantly lower than those in the control group after rehabilitation treatment(P<0.05). The Mayo elbow performance scores(MEPS), PFG scores and ADL scale scores in both groups at the 2nd week of rehabilitation treatment and at the 4th week of rehabilitation treatment were significantly higher than those before rehabilitation treatment(P<0.05), and the scores in the observation group were significantly higher than those in the control group after rehabilitation treatment(P<0.05). The disabilities of the arm, shoulder and hand(DASH) scores in both groups at the 2nd week of rehabilitation treatment and at the 4th week of rehabilitation treatment were significantly lower than those before rehabilitation treatment(P<0.05), and the scores in the observation group were significantly lower than those in the control group after rehabilitation treatment(P<0.05). The excellent and good rate of elbow joint function recovery in the observation group was significantly higher than that in the control group(92.45% vs 77.36%, χ²=4.711, P=0.030). During the postoperative rehabilitation treatment period, there were no complications such as incision infection, vascular and nerve damage, local swelling of the elbow joints and pain aggravation in both groups. Conclusion　The musculoskeletal ultrasound-guided injection of PRP combined with routine rehabilitation training can significantly improve LE patients′ upper limb function and elbow joint function after surgery, reduce their pain intensity and inflammatory state, and is safe and effective.]]> 2025/4/30 9:48:46 ZHU Lina, LI Haize, LIU Yajuan, LIU Hongxin 131true ［Abstract］　Objective　To analyze the characteristics of lung ultrasound(LUS) images of common lung diseases in newborns. Methods　A total of 466 newborns with suspected lung diseases who were born in Dehong Hospital Affiliated to Kunming Medical University(Dehong Prefecture People′s Hospital) from March 2018 to January 2024 were recruited. The newborns received both chest X-ray examination and LUS examination, with the time interval between the two examinations equal to or less than 15 minutes. Based on clinical manifestations, the diagnostic results of the two examinations were consistent. The characteristics of LUS images in different types of newborns were concluded and summarized. Results　Among the 466 newborns, there were 88 cases of transient tachypnea of newborn(TTN), 69 cases of neonatal respiratory distress syndrome(NRDS), 29 cases of neonatal acute respiratory distress syndrome(ARDS), 57 cases of meconium aspiration syndrome(MAS), 89 cases of neonatal pneumonia(NP), 6 cases of neonatal pulmonary hemorrhage(NPH), 20 cases of bronchopulmonary dysplasia(BPD), 3 cases of neonatal pulmonary atelectasis, 8 cases of neonatal pneumothorax, and 97 case of non-pulmonary diseases. The characteristics of LUS images of normal lungs in newborns: no abnormalities in pleural line and A-line, presence of lung slippage; manifestation of beach sign in M-mode ultrasound; absence of alveolar-interstitial syndrome(AIS), dense B-line, white lung and lung consolidation; possible B-line/comet-tail sign. The characteristics of LUS images of TTN: having abnormality in pleural line, abnormality in A-line, beach sign; having one or more than one feature of AIS/dense B-line/white lung, bipulmonary dots, B-line/comet-tail sign; possible lung islands and pleural effusion. The characteristics of LUS images of NRDS, ARDS, MAS, NP, NPH and BPD: having one of the manifestations of lung consolidation, fragment sign, ground-glass sign, snowflake sign, pulmonary pulsation and beach sign, and having the possible features of B-line/comet-tail sign, AIS/dense B-line/white lung, bipulmonary dots, abnormality in pleural line, abnormality in A-line and pleural effusion; and having the common manifestation of lung consolidation in LUS images. The LUS images of neonatal pulmonary atelectasis were manifested as lung consolidation, but no bipulmonary dots and pleural effusion were observed. The characteristics of LUS images of neonatal pneumothorax: presence of abnormality in lung slippage, stratosphere sign and possible bipulmonary dots. Conclusion　LUS can be used for the examination and diagnosis of common lung diseases in newborns. However, the same disease may have multiple characteristics of ultrasound images simultaneously, and the same characteristics of ultrasound images may also indicate different diseases. Clinicians should make the diagnosis for the newborn patients based on their clinical manifestations in combination with other examination results.]]> 2025/4/30 9:48:46 1,2, YANG Mingjie³, HUANG Na^1,2,4, ZHANG Meiling², XU Fang⁵, DUAN Shengluan⁵, LI Rui², GUO Yunhua³]]> FENG Zaili^1,2, YANG Mingjie³, HUANG Na^1,2,4, ZHANG Meiling², XU Fang⁵, DUAN Shengluan⁵, LI Rui², GUO Yunhua³ 130true ［Abstract］　Objective　To observe the effects of repetitive peripheral magnetic stimulation(rPMS) combined with conventional rehabilitation training on lower limb motor function after treatment of botulinum toxin type A(BoNT-A) in children with bilateral spastic cerebral palsy. Methods　Sixty pediatric patients with bilateral spastic cerebral palsy who were admitted to Xuzhou Children′s Hospital Affiliated to Xuzhou Medical University from June 2023 to June 2024 were recruited. All the pediatric patients were treated with BoNT-A after enrollment. The pediatric patients were divided into observation group(undergoing rPMS combined with conventional rehabilitation training) and control group(undergoing conventional rehabilitation training) by using random number table method, with 30 cases in each group. The pediatric patients′ balance function was assessed by using Pediatric Balance Scale(PBS) scores, and their lower limb motor function was assessed by using the Gross Motor Function Measure-88 Dimensions D and E(GMFM-88 D/E) scores, and their walking ability was assessed by using 1-Minute Walk Test(1MWT) before treatment of BoNT-A and 3 months after rehabilitation therapy, respectively. Results　Before treatment of BoNT-A, there were no significant differences in PBS scores, 1-minute walking distance, GMFM-88 D/E scores and total scores between the two groups(P>0.05). After 3 months of rehabilitation therapy, the PBS scores, GMFM-88 D/E scores and total scores in both groups were higher than those before treatment of BoNT-A, and the PBS scores, GMFM-88 D/E scores and total scores in the observation group were higher than those in the control group, and the 1-minute walking distance in both groups was longer than that before treatment of BoNT-A, and the 1-minute walking distance in the observation group was longer than that in the control group, with statistically significant differences between the two groups(P<0.05). Conclusion　After treatment of BoNT-A, the pediatric patients with bilateral spastic cerebral palsy who are assisted with rPMS combined with conventional rehabilitation training can get the improvement in their walking stability and lower limb motor function.]]> 2025/4/30 0:00:00 WANG LI, LI Xinjian, WU Ye, QIU Aizhen, ZHANG Mengran 129true ［Abstract］　Objective　To observe the application effect of Goals-Activity-Motor Enrichment(GAME) therapy in upper limb functional rehabilitation for cerebral palsy pediatric patients with spastic hemiplegia at preschool age. Methods　A total of 56 cerebral palsy pediatric patients with spastic hemiplegia at preschool age who were admitted to Beijing Children′s Hospital, Capital Medical University from August 2023 to July 2024 were recruited. The pediatric patients were divided into observation group and control group by random number table method, with 28 cases in each group. In the control group, the training was stopped in 2 patients due to their illness, and 1 patient was transferred to another place outside Beijing for continuous rehabilitation. In the observation group, 3 patients were transferred to other places outside Beijing for continuous rehabilitation. Finally, 25 cases in the observation group and 25 cases in the control group were included in this study. The pediatric patients in both groups received rehabilitation treatment through the combination of hospital rehabilitation and family training. The control group received constraint-induced movement therapy(CIMT). According to the principle of GAME therapy, the training program of the observation group was designed and the training was carried out. The rehabilitation treatment lasted for 3 months. Carroll Upper Extremity Function Test(UEFT) scores and Pediatric Evaluation of Disability Inventory(PEDI) standard scores were compared between the two groups before and after treatment. Results　Before treatment, the UEFT scores and PEDI standard scores were not significantly different between the two groups(P>0.05). After treatment, the UEFT scores and PEDI standard scores in both groups were elevated compared with those before treatment, and the UEFT scores and PEDI standard scores in the observation group were higher than those in the control group after treatment, with statistically significant differences between the two groups(P<0.05). Conclusion　The application of GAME therapy in upper limb functional training for cerebral palsy pediatric patients with spastic hemiplegia at preschool age can better improve the upper limb function and improve their self-care ability.]]> 2025/4/30 9:48:46 XU Xiaomeng, WANG Yan, LI Yiqing, LI Fengyan, ZHANG Chen, LI He, LI Haitian 128true ［Abstract］　Objective　To explore the application value of resting-state functional magnetic resonance imaging in observation of brain structure in patients with obstructive sleep apnea syndrome(OSAS). Methods　A total of 36 patients with OSAS who were admitted to the Second People′s Hospital of Hefei from June 2022 to June 2024 were recruited as observation group, and 36 subjects undergoing healthy physical examination as control group. Polysomnography(PSG) was used to monitor the research subjects′ sleep quality. The changes of cerebral gray matter volume(GMV) in different brain regions of the observation group were analyzed by scanning the research subjects′ heads with resting-state functional magnetic resonance imaging and using voxel-based morphometry(VBM) to obtain GMV. The research subjects were assessed by using Epworth Sleepiness Scale(ESS), Montreal Cognitive Assessment(MoCA), Mini-Mental State Examination(MMSE), Hamilton Depression Scale(HAMD) and Hamilton Anxiety Scale(HAMA). Pearson correlation analysis was used to explore the correlation between the GMV of right superior frontal gyrus and MoCA scores. Results　The apnoea hypopnoea index(AHI) and number of wake after sleep onset(WASO) in the observation group were larger than those in the control group, and the average oxygen concentration in the blood, minimum oxygen concentration in the blood and proportion of deep sleep phase in the observation group were lower than those in the control group, with statistically significant differences between the two groups(P<0.05). There was no significant difference in the proportion of light sleep phase between the two groups(P>0.05). The HAMD scores, HAMA scores and ESS scores in the observation group were higher than those in the control group, while the MoCA scores and MMSE scores in the observation group were lower than those in the control group, with statistically significant differences between the two groups(P<0.05). Compared with that in the control group, the GMV of bilateral superior temporal gyrus, bilateral thalamus, right superior frontal gyrus, left postcentral gyrus, and right precentral gyrus in the observation group was significantly decreased, and no brain regions with increased GMV were found. The GMV of the right superior frontal gyrus was positively correlated with the MoCA scores(r=0.380, P=0.011). Conclusion　Resting-state functional magnetic resonance imaging is helpful for early detection of brain structural and functional abnormalities in OSAS patients, which guides early clinical intervention and improves the patients′ prognosis.]]> 2025/4/30 0:00:00 YUAN Ye, SHENG Mao, WANG Song, CUI Ping, LIAN Hu, WU Hui, ZHONG Xing, XIA Jinxiang, LI Chuanmin 127true ［Abstract］　Objective　To explore the effects of emergency oocyte vitrification technique on embryo quality and developmental potential in assisted reproductive technology. Methods　The clinical data of 55 patients(55 cycles, egg freezing group) who underwent emergency oocyte vitrification in the Reproduction Hospital of Guangxi Zhuang Autonomous Region from April 2010 to May 2023 were retrospectively analyzed, and other 201 patients(201 cycles) who underwent intracytoplasmic sperm injection(ICSI) using fresh oocytes during the same period were randomly selected as control group. The results of ICSI after resuscitation of frozen oocytes were analyzed, and fertilization, embryo development and clinical pregnancy outcomes were compared between the two groups. Results　A total of 55 cycles of oocytes were frozen, of which 12 cycles were still frozen without being thawed, and the remaining 43 cycles had a total of 450 oocytes and all of them were thawed, of which 400 oocytes survived, and the recovery rate was 88.89%. The number of transplanted embryos in the egg freezing group was larger than that in the control group, but the high-quality embryo rate, blastocyst formation rate and available blastocyst rate were lower than those in the control group, with significant differences between the two groups(P<0.05). There were no significant differences in fertilization rate and normal cleavage rate between the two groups(P>0.05). The cycle rate of untransplantable embryos, clinical pregnancy rate and ectopic pregnancy rate in the egg freezing group were lower than those in the control group, but the differences were not statistically significant(P>0.05). The transplanting rate of the egg freezing group was significantly lower than that of the control group(P<0.05). There were no significant differences in abortion rate, live birth rate, gestational week, birth weight and birth defect rate between the two groups(P>0.05). Conclusion　Emergency oocyte vitrification may cause some damages to the embryos, but their clinical outcomes are still similar to those of fresh oocytes. Emergency oocyte vitrification is a remedy for the absence of sperms on the day of egg retrieval and an effective way to preserve female fertility.]]> 2025/4/30 9:48:46 GONG Fangqiang, HUANG Hua, LAO Yanping, SHI Li, NIU Xiangli 126true ［Abstract］　Objective　To analyze the clinical efficacy of percutaneous endocardial septal radiofrequency ablation(PESA) in treatment of hypertrophic obstructive cardiomyopathy(HOCM). Methods　The clinical data of 11 patients with HOCM who received PESA treatment in Department of Cardiovascular Medicine, Yantai Yuhuangding Hospital Affiliated to Qingdao University from September 2020 to June 2022 were retrospectively analyzed. The results of echocardiography, Kansas City Cardiomyopathy Questionnaire(KCCQ) scores, sudden cardiac death index(SCDI), New York Heart Association(NYHA) cardiac function grades in the patients were compared before surgery and 1 to 3 days after surgery, 1 to 3 months after surgery and 6 to 9 months after surgery and the occurrence of complications was recorded. Results　Among the 11 patients, there were 2 males and 9 females with an average age of (64.00±10.20)years. Compared with those before surgery, the patients′ left ventricular outflow tract pressure gradient(LVOTPG) levels were significantly decreased(P<0.05), while their interventricular septum thickness(IVST), left ventricular mass index(LVMI), left atrial diameter(LAD), left ventricular posterior wall thickness(LVPW), left ventricular end-diastolic diameter(LVEDD), left ventricular ejection fraction(LVEF), early diastolic mitral inflow velocity(E), early diastolic mitral annular velocity(e′), and E/e′ ratio did not change significantly after surgery(P>0.05). Compared with those before surgery, the patients′ physical limitation scores, self-evaluation scores, symptom scores, self-awareness scores, psychological scores, social function scores, clinical scores and total scores were significantly increased after surgery(P<0.05). Compared with those before surgery, the patients′ NYHA cardiac function grades were significantly improved(P<0.05), and their SCDI levels were significantly decreased(P<0.05). One case of complete left bundle branch block and 1 case of atrial premature contraction were observed during postoperative follow-up. Conclusion　PESA can significantly improve HOCM patients′ clinical symptoms and enhance their quality of life with fewer complications, providing a safe and effective option for HOCM treatment.]]> 2025/3/28 8:07:34 1, LIU Yugang², WANG Hua², WANG Guangqiang², REN Mengmeng², ZHONG Lin²]]> LUAN Mingwei¹, LIU Yugang², WANG Hua², WANG Guangqiang², REN Mengmeng², ZHONG Lin² 125true ［Abstract］　Objective　To analyze the effects of female age at the time of oocyte cryopreservation on embryonic development and clinical pregnancy outcomes. Methods　The clinical data of women who underwent oocyte vitrification freezing and thawing, and received intracytoplasmic sperm injection(ICSI) assisted pregnancy cycle in Women′s Hospital of Nanjing Medical University from January 2013 to May 2024 were retrospectively analyzed. According to the women′s age at the time of oocyte retrieval, they were divided into women <35 years old group(67 cases) and women ≥35 years old group(21 cases). The general clinical data, embryonic development and clinical pregnancy outcomes were compared between the two groups. Results　The level of body mass index(BMI) in the women ≥35 years old group was significantly higher than that in the women <35 years old group(P<0.05). There were no significant differences in basal follicle-stimulating hormone(FSH), basal estradiol(E₂), basal progesterone(P), basal luteinizing hormone(LH), basal testosterone(T) and superovulation protocols between the two groups(P>0.05). The level of LH on human chorionic gonadotropin(HCG) trigger day in the women≥35 years old group was significantly higher than that in the women <35 years old group(P<0.05), while there were no significant differences in the levels of E₂ and P on HCG trigger day between the two groups(P>0.05). There were no statistically significant differences between the two groups in oocyte survival rate, normal fertilization rate, oocyte degeneration rate after ICSI, 2 pronuclei(2PN) cleavage rate, usable embryo rate, high-quality embryo rate and blastocyst formation rate(P>0.05). After adjusting for confounders including BMI, endometrial thickness on HCG trigger day, embryo transfer stage, number of transferred embryos, and number of high-quality embryos transferred, the results of multivariate logistic regression analysis showed that compared with those <35 years old, the women ≥35 years old had significantly lower probabilities of fresh embryo live birth and cumulative live birth(P<0.05). Conclusion　Increasing the female age at the time of oocyte cryopreservation may reduce the rate of live birth after embryo transfer.]]> 2025/3/28 8:07:34 HU Qingyun, JI Hui, ZHANG Junqiang, ZHAO Chun, LANG Peng, LING Xiufeng 124true ［Abstract］　Objective　To screen the biomarkers related to early recurrence of hepatocellular carcinoma(HCC) and to analyze their significance in predicting the HCC patients′ prognosis. Methods　Based on the previous studies, 16 differentially expressed genes(DEGs) were screened, including human leukocyte antigen-A(HLA-A), interleukin-23 alpha(IL-23A), tumor protein 63(TP63), arachidonate 15-lipoxygenase type B(ALOX15B), budding uninhibited by benzimidazoles 1(BUB1), C-X-C motif chemokine receptor 2(CXCR2), C-C motif chemokine ligand 20(CCL20), C-type lectin domain family 4 member C(CLEC4C), protein tyrosine kinase 7(PTK7), myeloperoxidase(MPO), interleukin-1 beta(IL-1β), matrix metallopeptidase 9(MMP9), G antigen 2A(GAGE2A), G antigen 2E(GAGE2E), deleted in malignant brain tumors 1(DMBT1), and forkhead box M1(FOXM1). The Cancer Genome Atlas(TCGA) database was searched with the keyword “TCGA-LIHC”, and a total of 374 hepatocellular carcinoma tissues and 50 control tissues(all belonging to cancer-adjacent tissues) were obtained. The RNA databases and their clinical data were downloaded. The expression differences of DEGs in the hepatocellular carcinoma tissues and the cancer-adjacent tissues were analyzed. Gene Ontology(GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis were performed on the DEGs. A protein-protein interaction networks(PPI) was constructed by using the STRING online database. The surv_cutpoint function in the R 4.1.2 software package “survminer” was applied to obtain the optimum cut-off value, and the the optimum cut-off value was used to distinguish the high expression group from the low expression group. The relationship between the DEGs and the patients′ survival prognosis was analyzed by using Kaplan-Meier survival curves. Cox regression was used to analyze the factors affecting the HCC patients′ prognosis. Results　Compared with those in the cancer-adjacent tissues, HLA-A, IL-23A, TP63, ALOX15B, BUB1, CCL20, PTK7, MMP9, GAGE2A, DMBT1 and FOXM1 in the hepatocellular carcinoma tissues were highly expressed, while MPO, IL-1β, CXCR2 and CLEC4C in the hepatocellular carcinoma tissues were low expressed, and the differences were statistically significant(P<0.05). There was no significant difference in the expression of GAGE2E between the hepatocellular carcinoma tissues and the cancer-adjacent tissues(P>0.05). The results of GO enrichment analysis indicated that the changes in biological process(BP) of DEGs were significantly enriched in neutrophil migration, granulocyte chemotaxis and neutrophil chemotaxis. The changes in cellular components(CC) were predominantly enriched in secretory granule membranes, phagocytic vesicles and phagocytic vesicle membranes. The changes in molecular functions(MF) were enriched in receptor-ligand activity, cytokine receptor binding and cytokine activity. The results of KEGG pathway enrichment analysis revealed that the DEGs were primarily enriched in the tumor necrosis factor(TNF) signaling pathway, interleukin-17(IL-17) signaling pathway and rheumatoid arthritis pathway. The results of PPI analysis showed that the top 5 protein pairs with the highest protein-protein interaction scores were MPO-MMP9(scores: 0.969), CCL20-IL-1β(scores: 0.958), CCL20-CXCR2(scores: 0.949), MMP9-IL-1β(scores: 0.949), and IL-1β-MPO(scores: 0.860). The results of Kaplan-Meier survival curve analysis indicated that the survival prognoses of the high expression groups of BUB1, MMP9, CCL20, PTK7 IL-1β and FOXM1 were significantly better than those of their corresponding low expression groups(P<0.05). The results of multivariate Cox regression analysis indicated that T₃/T₄ stage, tumor-bearing status and high FOXM1 expression were independent risk factors for overall survival(OS) of the HCC patients(P<0.05). Conclusion　DEGs associated with early recurrence of HCC are mainly involved in the pathways related to inflammation and immune responses. FOXM1 may serve as a biological marker for predicting the survival prognosis of the HCC patients.]]> 2025/3/28 8:07:34 1,2, CHEN Kehe^1,2, CAO Yuhua², PAN Deng², WANG Junping², OU Mei², ZHONG Wenhe², GAO Ting², WEI Haiming²]]> LIANG Meng^1,2, CHEN Kehe^1,2, CAO Yuhua², PAN Deng², WANG Junping², OU Mei², ZHONG Wenhe², GAO Ting², WEI Haiming² 123true ［Abstract］　Objective　To analyze the therapeutic effects of primary motor cortex(M1) high-frequency combined with supplementary motor area(SMA) low-frequency repetitive transcranial magnetic stimulation(rTMS) assisting with drug in treatment of elderly Parkinson′s disease based on neurotransmitter and computed tomography(CT) cerebral blood flow perfusion and their mechanisms. Methods　One hundred and six patients with elderly Parkinson′s disease who were admitted to Yan ′an University Xianyang Hospital from September 2021 to June 2023 were recruited and divided into observation group and control group by random number table method, with 53 cases in each group. Both groups were given pramipexole tablets, and on this basis, the control group was given sham stimulation+memantine, while the observation group was given rTMS+memantine. Both groups were treated for 4 weeks. The Unified Parkinson′s Disease Rating Scale(UPDRS) scores, Mini-Mental State Examination(MMSE) scores, Hamilton Rating Scale for Anxiety(HAMA) scores, Hamilton Rating Scale for Depression(HAMD) scores, P300 amplitude and latency, neurotransmitter indicators［dopamine, 5-hydroxytryptamine, glutamic acid and gamma-aminobutyric acid(GABA)］, cerebral blood flow perfusion and the occurrence of adverse reactions were compared between the two groups. Results　After treatment, the UPDRS-Ⅱ scores, UPDRS-Ⅲ scores, UPDRS total scores, HAMA scores, HAMD scores and P300 latency in the observation group were lower than those in the control group, and the MMSE scores, P300 amplitude, dopamine, 5-hydroxytryptamine, glutamic acid, GABA, and the levels of cerebral blood flow perfusion in the temporal lobe, parietal lobe and frontal lobe in the observation group were higher than those in the control group, with statistically significant differences between the two groups(P<0.05). Conclusion　M1 high-frequency combined with SMA low-frequency rTMS assisting with drug can effectively improve the motor and non-motor symptoms in treatment of the patients with elderly Parkinson′s disease, regulate the levels of neurotransmitters, increase cerebral blood flow perfusion, and has good safety.]]> 2025/3/28 8:07:34 ZHANG Ruijuan, HE Lei 122true ［Abstract］　Objective　To explore the predictive value of C-reactive protein-albumin-lymphocyte(CALLY) index combined with neutrophil-to-lymphocyte ratio(NLR) for refractory Mycoplasma pneumoniae pneumonia(RMPP) and mechanical ventilation in children. Methods　A total of 236 pediatric patients with Mycoplasma pneumoniae pneumonia(MPP) who were hospitalized and treated in Department of Pediatrics, Tongzhou Maternal and Child Health Care Hospital of Beijing from January 2023 to June 2024 were selected.According to the occurrence of RMPP, the pediatric patients were divided into RMPP group(82 cases) and non-RMPP group(154 cases). The pediatric patients were divided into mechanical ventilation group(43 cases) and non-mechanical ventilation group(193 cases) according to whether mechanical ventilation was required for them during treatment. Multivariate logistic regression was used to analyze the influencing factors of RMPP. The value of CALLY index, NLR and their combination in predicting RMPP and mechanical ventilation was analyzed by using receiver operating characteristic(ROC) curve. Results　The patients of the RMPP group were older than those of the non-RMPP group, and the levels of C-reactive protein, procalcitonin, lactate dehydrogenase, white blood cell count, neutrophil count and NLR in the RMPP group were higher than those in the non-RMPP group. The hospitalization time of the RMPP group was longer than that of the non-RMPP group, and the levels of albumin, lymphocyte count and CALLY index in the RMPP group were lower than those in the non-RMPP group. The above differences were statistically significant between the two groups(P<0.05). There were significant differences in antibiotic use, mechanical ventilation rate and treatment effect between the two groups(P<0.05). Older age and higher levels of NLR, lactate dehydrogenase and procalcitonin were independent risk factors for the occurrence of RMPP(P<0.05), and higher CALLY index was independent protective factor for the occurrence of RMPP(P<0.05). Both CALLY index and NLR were helpful in predicting RMPP(P<0.05), and the combination of the two indicators had a higher predictive efficacy［AUC(95%CI)=0.927(0.886-0.957)］ than a single indicator(CALLY index or NLR)(P<0.05), with a sensitivity of 79.32% and a specificity of 92.91%. The NLR of the mechanical ventilation group was higher than that of the non-mechanical ventilation group, and the CALLY index of the mechanical ventilation group was lower than that of the non-mechanical ventilation group, with statistically significant differences between the two groups(P<0.05). Both CALLY index and NLR were helpful in predicting mechanical ventilation(P<0.05), and the combination of the two indicators had a higher predictive efficacy［AUC(95%CI)=0.828(0.774-0.874)］ than a single indicator(CALLY index or NLR)(P<0.05), with a sensitivity of 83.71% and a specificity of 77.68%. Conclusion　CALLY index combined with NLR can be used as an effective indicator for predicting RMPP and mechanical ventilation in children. The combined detection of CALLY index and NLR can help to identify high-risk pediatric patients early, guide clinical decision-making and improve prognosis.]]> 2025/3/28 8:07:34 ZHU Yuxin, YANG Li 121true ［Abstract］　Objective　To construct and validate a Nomogram model for predicting the outcome of facial nerve function(FNF) after acoustic neuroma(AN) surgery. Methods　The clinical data of 124 patients with AN who were admitted to Department of Neurosurgery, the First Teaching Hospital of Xinjiang Medical University from September 2020 to December 2023 were collected. All the patients underwent tumor resection through the retrosigmoid sinus approach. Multivariate logistic regression analysis was used to screen factors affecting the outcome of FNF after AN surgery, and these factors were incorporated to construct a Nomogram model. Internal validation was conducted for the Nomogram model. Results　Among the 124 patients with AN, the prognosis of FNF after surgery was good in 57 cases(good prognosis group) and poor in 67 cases(poor prognosis group). Compared with the good prognosis group, the poor prognosis group was older, and had larger tumor diameter, anterior tumor length and posterior tumor length, and higher proportion of patients with moderate to severe tumor adhesion to facial nerve and cystic tumor in nature, with statistically significant differences between the two groups(P<0.05). The results of multivariate logistic regression analysis showed that older age［OR(95%CI)=1.078(1.025-1.134)］, larger tumor diameter［OR(95%CI)=2.231(1.274-3.906)］, cystic tumor in nature［OR(95%CI)=7.601(2.212-26.124)］, moderate tumor adhesion to facial nerve［OR(95%CI)=11.182(3.023-41.364)］ and severe tumor adhesion to facial nerve［OR(95%CI)=86.653(13.414-559.774)］ were independent risk factors for poor prognosis of FNF in the AN patients after surgery(P<0.05). Age, tumor nature, tumor diameter, and the severity of tumor adhesion to facial nerve were used as 4 variables to construct the Nomogram model. The results of receiver operating characteristic(ROC) curve analysis showed that area under the curve(AUC) of the model was 0.920(95%CI: 0.869-0.971), indicating that the model had relatively high predictive efficiency. The calibration degree of the model was validated by Bootstrap resampling method(1 000 times), and the calibration curve was found to be close to the ideal curve, indicating that the model had relatively good predictive value. The analytic results of decision-making analysis curve(DAC) showed that the DAC was above the None line and All line at the threshold range of 5%-65%, suggesting that the model had relatively good clinical practicability. Conclusion　The Nomogram model constructed in this study can effectively predict the outcome of FNF after AN surgery, and has clinical application value.]]> 2025/3/28 0:00:00 KUAI Guohu, LIAN Minghao, LI Yandong, ZHANG Gaocai, ZHU Guohua, Maimaitili Mijiti, Geng Dangmurenjiafu 120true ［Abstract］　Objective　To analyze the predictive efficacy of human neutrophil peptide-1(HNP-1), human neutrophil peptide-2(HNP-2) and human cathelicidin LL-37 levels in sputum for acute exacerbation of bronchiectasis. Methods　A total of 129 patients with stable bronchiectasis who were admitted to Hebei Chest Hospital from September 2021 to June 2023 were recruited. Before treatment, sputum samples of the patients were collected by natural cough method, and the levels of HNP-1, HNP-2 and human cathelicidin LL-37 were detected by using enzyme-linked immunosorbent assay(ELISA). According to the occurrence of acute exacerbation within 6 months of follow-up, the patients were divided into acute exacerbation group(48 cases) and non-exacerbation group(81 cases). The correlations of HNP-1, HNP-2 and human cathelicidin LL-37 levels with enhanced FACED(E-FACED) scores and lung function were analyzed by using Pearson correlation analysis. Multivariate logistic regression was used to analyze the influencing factors for acute exacerbation of bronchiectasis. The efficacy of HNP-1, HNP-2 and human cathelicidin LL-37 levels in predicting acute exacerbation of bronchiectasis was analyzed by using receiver operating characteristic(ROC) curve. Results　The proportion of patients smoking, the proportion of patients with diabetes, the E-FACED scores, and the levels of HNP-1, HNP-2 and human cathelicidin LL-37 in the acute exacerbation group were higher than those in the non-exacerbation group, and the forced expiratory volume in 1 second(FEV₁) as percentage of predicted value(FEV₁%pred) in the acute exacerbation group were lower than those in the non-exacerbation group, with statistically significant differences between the two groups(P<0.05). Levels of HNP-1, HNP-2 and human cathelicidin LL-37 were positively correlated with E-FACED scores(P<0.05) and were negatively correlated with FEV₁%pred(P<0.05). The results of multivariate logistic regression analysis showed that smoking history, diabetes and higher HNP-1, HNP-2 and human cathelicidin LL-37 levels were independent risk factors for acute exacerbation of bronchiectasis(P<0.05). The results of ROC curve analysis showed that HNP-1, HNP-2 and human cathelicidin LL-37 levels were helpful for predicting acute exacerbation of bronchiectasis(P<0.05), and the predictive efficacy of the combination of the three indicators［AUC(95%CI)=0.908(0.845-0.952)］ was higher than that of the single indicator(P<0.001), with a sensitivity of 75.00% and a specificity of 93.83%. Conclusion　Levels of HNP-1, HNP-2 and human cathelicidin LL-37 in sputum are all associated with acute exacerbations of bronchiectasis, and the combination of the three indicators can improve the predictive efficacy for acute exacerbation of bronchiectasis.]]> 2025/3/28 8:07:34 NI Weibo, GUO Lijuan, DENG Ye, BAO Jie, SHANG Huanxia 119true ［Abstract］　Objective　To explore the value of the prediction of dorsal intra-articular bone fragment stability following simple volar plate internal fixation based on the anatomic indicators of computed tomography(CT) Materialise′s interactive medical image control system(Mimics) three-dimensional reconstruction. Methods　The clinical data of 165 patients with fractures of distal radius who were treated with simple volar plate internal fixation in Chongqing Jiulongpo Traditional Chinese Medicine Hospital from September 2021 to June 2023 were retrospectively analyzed. The patients were divided into a displacement group(30 cases, displaced dorsal intra-articular bone fragments >2 mm) and a non-displacement group(135 cases, displaced dorsal intra-articular bone fragments ≤2 mm) according to the displaced dorsal intra-articular bone fragments on the 2nd day after surgery and during outpatient follow-up of 6 months after surgery. The general clinical data and the anatomic indicator levels of CT Mimics three-dimensional reconstruction before surgery were compared between the two groups, and the correlation between each preoperative anatomical indicator and the distance of the displaced dorsal intra-articular bone fragments was analyzed. Multivariate logistic regression analysis was used to analyze the factors affecting the stability of the dorsal intra-articular bone fragments after surgery, and a Nomogram model was constructed based on the screened indicators. The application value of the Nomogram model was evaluated by using receiver operating characteristic(ROC) curve, calibration curve, and decision-making analysis curve. Results　The proportion of patients with fracture AO classification of C3 in the displacement group was higher than that in the non-displacement group, with statistically significant difference betweent the two groups(P<0.05). The volume, transverse to longitudinal diameter ratio, and transverse width of the bone fragments in the displacement group were smaller than those in the non-displacement group, while the total distance of the displaced bone fragments before surgery in the displacement group was greater than that in the non-displacement group, with statistically significant differences betweent the two groups(P<0.05). The volume of bone fragments(r=-0.817, P<0.001), transverse to longitudinal diameter ratio of bone fragments(r=-0.771, P<0.001), and transverse width of bone fragments(r=-0.843, P<0.001) were negatively correlated with the distance of the displaced dorsal intra-articular bone fragments after surgery, and the total distance of the displaced bone fragments before surgery was positively correlated with the distance of the displaced dorsal intra-articular bone fragments after surgery(r=0.746, P<0.001). The results of multivariate logistic regression analysis showed that fracture AO classification of C3［OR(95%CI)=2.368(2.015-2.784)］, and longer total distance of the displaced bone fragments before surgery［OR(95%CI)=1.277(1.163-1.402)］ were independent risk factors for promoting the displaced dorsal intra-articular bone fragments after surgery(P<0.05), and larger volume of bone fragments［OR(95%CI)=0.464(0.268-0.802)］, larger transverse to longitudinal diameter ratio of bone fragments［OR(95%CI)=0.411(0.176-0.959)］ and larger transverse width of bone fragments［OR(95%CI)=0.421(0.225-0.786)］ were protective factors that inhibited the occurrence of postoperative displaced dorsal intra-articular bone fragments(P<0.05). Based on the fracture AO classification and the anatomic indicators of CT Mimics three-dimensional reconstruction before surgery, a Nomogram model for predicting postoperative displaced dorsal intra-articular bone fragments(C-index=0.857) was constructed. The results of receiver operating characteristic(ROC) curve, calibration curve, and decision-making analysis showed that this predictive model can effectively predict postoperative displaced dorsal intra-articular bone fragments in the patients, with good clinical application value. Conclusion　The volume of bone fragments, transverse to longitudinal diameter ratio of bone fragments, transverse width of bone fragments and the total distance of the displaced bone fragments before surgery can be used to predict the stability of the dorsal intra-articular bone fragments after simple volar plate internal fixation surgery, which can assist clinicians in early identification of high-risk patients and improve their prognosis.]]> 2025/3/28 8:07:34 1, LIU Xiaobo¹, GAO Dongfang², HE Xiaohao¹, SUN Shuxia¹]]> CHANG Juntao¹, LIU Xiaobo¹, GAO Dongfang², HE Xiaohao¹, SUN Shuxia¹ 118true ［Abstract］　Objective　To observe the application effect of pleurodesis in surgical treatment of spontaneous pneumothorax in adolescents. Methods　The clinical data of 187 adolescent patients with spontaneous pneumothorax who underwent video-assisted thoracoscopic bullotomy in Department of Cardiothoracic Surgery, Guangdong Provincial Hospital of Chinese Medicine from January 2014 to December 2022 were retrospectively analyzed. According to the different methods of pleural fixation during the surgery, the patients were divided into pleural friction group(group A, 50 cases), talc powder spray group(group B, 67 cases) and pleural non-fixation group(group C, 70 cases). The perioperative relevant indicators, postoperative adverse events and pneumothorax recurrence were compared among the three groups. Results　The surgical treatments were successfully completed in all the patients. The operative time of group C was significantly shorter than that of group A(P<0.05), and the length of hospitalization in group C was significantly shorter than that in group B(P<0.05). Compared with group A and group B, group C had less intraoperative blood loss, less postoperative drainage volume and lower usage rate of postoperative analgesics, and shorter time of chest tube placement after surgery, and the differences were statistically significant(P<0.05). There were no significant differences between group A and group B in the operative time, intraoperative blood loss, postoperative drainage volume and usage rate of postoperative analgesics, time of chest tube placement after surgery and the length of hospitalization(P>0.05). In group A, 2 patients needed a second operation for hemostasis due to hemorrhage in the pleural cavity, and in group B, 5 patients developed massive pleural effusion on the affected side 4 weeks after surgery. No adverse events were observed in group C. During the follow-up period, there were 2 cases(4.00%), 3 cases(4.48%) and 1 case(1.43%) of recurrent pneumothorax in group A, group B and group C, respectively, and there was no significant difference in the recurrence rate among the three groups(χ²=1.162, P=0.559). Conclusion　For adolescents with spontaneous pneumothorax, video-assisted thoracoscopic bullotomy combined with pleurodesis can not reduce the recurrence rate of pneumothorax and may increase the risk of adverse events.]]> 2025/3/28 8:07:34 LI Jia, ZHAO Qingwu, LI Yongfeng, CHEN Jicai, LIN Yu 117true ［Abstract］　Objective　To explore the mechanism of action of Houttuynia cordata Thunb. in treating bacterial keratitis(BK) and fungal keratitis(FK) based on network pharmacology technology. Methods　The main chemical components of Houttuynia cordata Thunb. were obtained from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP) database and chemical database. The action targets of the chemical components of Houttuynia cordata Thunb. were obtained from Swiss Target Prediction database and TargetNet database. GeneCards database and DisGeNET database were used to obtain the potential targets of BK and FK. Protein-protein interaction(PPI) network and “active ingredient-core target-key pathway” network were constructed by using STRING database and Cytoscape software. DAVID database was used to perform Gene Ontology(GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis on the potential targets of Houttuynia cordata Thunb. in treating BK and FK, and the mechanisms of action were predicted. Results　In this study, 14 main active ingredients of Houttuynia cordata Thunb. and 858 potential targets were screened out, and 70 potential targets for treating BK and 52 potential targets for treating FK were determined after analysis. The results of PPI analysis showed that 12 core proteins were related to the treatment of Houttuynia cordata Thunb. for BK and 15 core proteins were related to the treatment of Houttuynia cordata Thunb. for FK, and GO enrichment analysis and KEGG pathway enrichment analysis revealed the related biological processes, molecular functions, cell composition and main pathways. The “active ingredient-core target-key pathway” network showed that quercetin in Houttuynia cordata Thunb. was the key active compound in treating BK and FK. The key target of treating BK was protein kinase B1(AKT1), and the key target of treating FK was mitogen-activated protein kinase 14(MAPK14). The nuclear factor-kappa B(NF-κB) signaling pathway was their common key action pathway. Conclusion　Houttuynia cordata Thunb. has the characteristics of synergistic effects of multiple components, multiple targets and multiple pathways in the treatments of BK and FK, and quercetin is the core active ingredient.]]> 2025/3/28 8:07:34 LU Honglong, ZOU Lianjia, LI Xuezheng 116true ［Abstract］　Objective　To analyze the expression level and clinical application value of serum canopy fibroblast growth factor signaling regulator 2(CNPY2) in gastric cancer. Methods　The clinical data of 65 gastric cancer patients hospitalized in the Department of Gastrointestinal Surgery of Shandong Provincial Hospital Affiliated to Shandong First Medical University from December 2023 to September 2024 were collected. Among the patients, there were 39 cases of early gastric cancer(TNM stage Ⅰ) and 26 cases of progressive gastric cancer(6 cases of TNM stage Ⅱ, 17 cases of TNM stage Ⅲ and 3 cases of TNM stage Ⅳ). Other 43 cases of patients with benign gastric diseases in Shandong Provincial Hospital Affiliated to Shandong First Medical University were selected as the benign group during the same period, and 52 cases of healthy individuals undergoing physical examination were selected as the control group. The levels of serum CNPY2, carcinoembryonic antigen(CEA) and carbohydrate antigen 199(CA199) were detected. The diagnostic efficacy of CNPY2, CEA, and CA199 for gastric cancer and early gastric cancer was analyzed by using receiver operating characteristic(ROC) curve. Results　The median concentration of CNPY2 in the gastric cancer group(0.26 ng/mL) was higher than that in the benign group(0.21 ng/mL) and the control group(0.21 ng/mL), and the median concentration of CEA in the gastric cancer group(2.21 ng/mL) was higher than that in the benign group(1.28 ng/mL) and the control group(1.32 ng/mL), and the median concentration of CA199 in the gastric cancer group(13.70 IU/mL) was higher than that in the control group(9.26 IU/mL), and the differences were statistically significant(P<0.05). The median concentrations of CNPY2(0.27 ng/mL) and CA199(16.95 IU/mL) in gastric cancer patients with TNM stage Ⅱ to stage Ⅳ(22 cases) were higher than the median concentrations of CNPY2(0.24 ng/mL) and CA199(11.05 IU/mL) in gastric cancer patients with TNM stage Ⅰ(36 cases), and the patients with poorly differentiated gastric cancer(34 cases) had a higher median concentration of CNPY2(0.27 ng/mL) than the patients with moderately to highly differentiated gastric cancer(24 cases)(0.25 ng/mL), and the median concentration of CEA(3.44 ng/mL) in the gastric cancer patients with human epidermal growth factor receptor 2(HER2) negative(18 cases) was higher than the median concentration of CEA(1.88 ng/mL) in the gastric cancer patients with HER2 positive(40 cases), and the differences were statistically significant(P<0.05). The results of ROC curve analysis showed that using the combination of CNPY2, CEA and CA199 in diagnosis of gastric cancer［AUC(95%CI)=0.885(0.823-0.947)］ and early gastric cancer［AUC(95%CI)=0.857(0.780-0.933)］ had higher efficacy than using CNPY2, CEA and CA199 alone(P<0.05). Conclusion　Serum CNPY2 levels are significantly elevated in patients with gastric cancer, and the combined detection of serum CNPY2, CEA and CA199 can improve the diagnostic efficacy of gastric cancer and early gastric cancer.]]> 2025/3/1 0:00:00 1,2, JU Ying², LIU Duanrui², LIU Yiqing², LIU Yun², ZHANG Peili^1,2, PENG Meiyu¹]]> CHEN Lanlan^1,2, JU Ying², LIU Duanrui², LIU Yiqing², LIU Yun², ZHANG Peili^1,2, PENG Meiyu¹ 115true ［Abstract］　Objective　To explore the expression levels of serum claudin-5 and c-Jun N-terminal kinase activating protein(JKAP) in patients with Parkinson′s disease(PD) and their clinical significance. Methods　A total of 85 PD patients(PD group) admitted to the Department of Neurology of Bao Ji People′s Hospital from January 2021 to July 2024 were selected. According to Hoehn-Yahr classification, the PD patients were divided into early stage PD group(grade 1-2.5, 17 cases), middle stage PD group(grade 3, 41 cases), and late stage PD group(grade 4-5, 27 cases). Other 85 volunteers who underwent physical examination in Bao Ji People′s Hospital during the same period were selected as the control group. The levels of claudin-5 and JKAP in serum were detected by using enzyme-linked immunosorbent assay. According to the scores of PD-Cognitive Rating Scale(PD-CRS), the PD patients were divided into normal cognitive group(>80 points, 28 cases), PD mild cognitive impairment(PD-MCI) group(74-80 points, 37 cases) and PD dementia(PDD) group(<74 points, 20 cases). Among them, PD-MCI and PDD belonged to PD cognitive impairment. Spearman rank correlation was used to analyse the correlation of claudin-5 and JKAP levels with the PD patients′ Hoehn-Yahr grades. Multivariate logistic regression was used to analyze the influencing factors of cognitive impairment in the PD patients. The efficacy of claudin-5 and JKAP levels for diagnosing cognitive impairment and PDD in the PD patients was analyzed by using receiver operating characteristic(ROC) curve. Results　Compared with the control group, the PD group had higher level of claudin-5 and lower level of JKAP, and the differences were statistically significant(P<0.05). The levels of claudin-5 in the early stage PD group, the middle stage PD group and the late stage PD group were increased in order, while the levels of JKAP in the early stage PD group, the middle stage PD group and the late stage PD group were decreased in order, and the differences were statistically significant(P<0.05). The Hoehn-Yahr grade in the PD patients was positively correlated with the claudin-5 level(r_s=0.810, P<0.001), and the Hoehn-Yahr grade in the PD patients was negatively correlated with the JKAP level(r_s=-0.823, P<0.001). The disease courses of the normal cognitive group, the PD-MCI group and the PDD group were prolonged in order, and the claudin-5 levels of the normal cognitive group, the PD-MCI group and the PDD group were increased in order, and the JKAP levels of the normal cognitive group, the PD-MCI group and the PDD group were decreased in order, and the differences were statistically significant(P<0.05). The results of multivariate logistic regression analysis showed that higher claudin-5 level was an independent risk factor for cognitive impairment in the PD patients(P<0.05), and higher JKAP level was an independent protective factor for cognitive impairment in the PD patients(P<0.05). The results of ROC curve analysis showed that the combination of claudin-5 and JKAP levels had higher diagnostic efficacy for cognitive impairment［AUC(95%CI)=0.894(0.808-0.950)］ and PDD［AUC(95%CI)=0.901(0.793-0.964)］ than the single indicator in the PD patients(P<0.05). Conclusion　The level of claudin-5 in the serum of PD patients is increased and the level of JKAP is decreased, which are related to the aggravation of the disease and cognitive impairment. The combined detection of the two indicators is helpful for the diagnosis of cognitive impairment in the PD patients.]]> 2025/3/1 12:31:01 BIAN Na, LIU Qian, SUN Yangyuan 114true ［Abstract］　Objective　To establish and validate the reference intervals of plasma aminogram of healthy adults in Shanghai area based on high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) technique. Methods　A total of 341 apparently healthy individuals who underwent physical examination at the Physical Examination Center of Huashan Hospital Affiliated to Fudan University from January 2023 to October 2023 were recruited. Other 40 apparently healthy individuals were recruited from November 2023 to December 2023 for reference interval validation. HPLC-MS/MS technique was used to detect the levels of 28 kinds of amino acids in the plasma of the study subjects. The reference intervals of plasma aminogram of healthy adults in Shanghai area were established and validated. Results　The methodological evaluation results of HPLC-MS/MS detection of adult plasma amino acids showed that the coefficient of variation of precision of the analytes was less than 15%, and the accuracy was between 86% and 115%, and the quantification lower limit deviation was less than 15%, and the coefficient of variation was less than 15%. The correlation coefficient(R²) of the linear range standard curve was greater than 0.99, and the validations of the carrying contamination rate and sample stability met the corresponding requirements. Based on this method, the reference intervals of 28 kinds of amino acids in the plasma of healthy adults in Shanghai area were established. The results of gender stratification analysis showed that the levels of most amino acids and metabolites in the plasma of healthy adult males were higher than those in the plasma of healthy adult females, and the differences were statistically significant(P<0.05). The validation results showed that the reference intervals established in this study met the requirements of Define and Determine the Reference Intervals in Clinical Laboratory(WS/T 402—2012) standard. Conclusion　In this study, the reference intervals of 28 kinds of plasma amino acids in healthy adults in Shanghai area are established and validated to provide a basis for clinical diagnosis and treatment.]]> 2025/3/1 0:00:00 1, ZHAN Yingying², ZHAO Yangyang³, HU Yao⁴, ZHANG Cuiping⁴]]> ZHU Xuan¹, ZHAN Yingying², ZHAO Yangyang³, HU Yao⁴, ZHANG Cuiping⁴ 113true ［Abstract］　Objective　To observe the efficacy of neuromuscular activation(Neurac) technique combined with transcranial magnetic stimulation(TMS) of vestibular reflex in treatment of post-stroke hemiplegia. Methods　A total of 123 patients with post-stroke hemiplegia who were admitted to Beijing Rehabilitation Hospital Affiliated to Capital Medical University from January 2022 to August 2023 were recruited. The patients were randomly divided into Neurac technique group(N group), TMS of vestibular reflex group(T group), and combined treatment group, with 41 cases in each group. All the patients received conventional rehabilitation training. On this basis, N group received treatment of Neurac technique, and T group received treatment of TMS of vestibular reflex, and the combined treatment group received a combined treatment of Neurac technique and TMS of vestibular reflex, and all the groups were treated continuously for 4 weeks. The trunk stability, walking function, surface electromyography indicators［root mean square(RMS) values of biceps brachii and triceps brachii］, lower limb muscle strength, upper and lower limb motor ability［Fugl-Meyer Assessment(FMA) scale scores］, body balance function［ Postural Assessment Scale for Stroke(PASS) scores, Brunel Balance Scale(BBA) scores］ and quality of life［Stroke-Specific Quality of Life Scale(SS-QOL) scores］ were compared among the three groups before and after treatment. Results　Before treatment, there were no statistically significant differences in the trunk stability indicators among the three groups(P>0.05). After treatment, the swing length, swing area, and average speed of the body in the three groups were significantly reduced when the eyes were open and closed compared with those before treatment(P<0.05), and the levels of the indicators in the combined treatment group were significantly lower than those in T group and N group(P<0.05), and the levels of the indicators in T group were significantly lower than those in N group(P<0.05). Before treatment, there were no statistically significant differences in RMS values of biceps brachii and triceps brachii at the state of elbow flexion and extension, lower limb muscle strength, FMA scores of upper and lower limbs, BBA scores, and PASS scores among the three groups(P>0.05). After treatment, the RMS values of biceps brachii and triceps brachii at the state of elbow flexion and extension, FMA scores of upper and lower limbs, BBA scores, and PASS scores in the three groups were significantly elevated compared with those before treatment(P<0.05), and the levels of the indicators in the combined treatment group were higher than those in N group and T group(P<0.05). However, there were no significant differences between N group and T group(P>0.05). After treatment, the lower limb muscle strength in the three groups improved significantly compared with that before treatment(P<0.05), and the improvement in the combined treatment group was significantly better than that in N group and T group(P<0.05). After treatment, the self-care ability, activity ability, physical ability, and social role dimension scores of SS-QOL in the combined treatment group were significantly higher than those in N group and T group(P<0.05), but there were no statistically significant differences between N group and T group(P>0.05). Conclusion　Neurac technique combined with TMS of vestibular reflex can enhance the muscle strength of the upper and lower limbs of the patients, improve their physical stability and balance ability, enhance their walking ability in the treatment of post-stroke hemiplegia, and improve their quality of life.]]> 2025/3/1 12:31:01 WU Dan, GUO Boyuan, HUANG Yan, JIA Nan, GAO Chang, LI Shuang, ZHANG Xiaoying 112true ［Abstract］　Objective　To construct a Nomogram prediction model for obstructive sleep apnea hypopnea syndrome(OSAHS) complicated with coronary heart disease(CHD) based on four-variable screening tool and conventional risk factors. Methods　A total of 1 224 OSAHS patients who were admitted to the First Teaching Hospital of Xinjiang Medical University from February 2020 to March 2024 were enrolled. The patients were randomly divided into a training set(857 cases) and a validation set(367 cases) in a ratio of 7∶3. According to the complicating CHD of the patients in the training set, these patients were divided into OSAHS+CHD group(277 cases) and OSAHS+non-CHD group(580 cases). The independent variables were screened by LASSO regression, and the influencing factors of OSAHS complicated with CHD were analyzed by using multivariate logistic regression, and a Nomogram prediction model was established. Receiver operating characteristic(ROC) curve, calibration curve, Hosmer-Lemeshow test, and decision curve analysis(DCA) were used to evaluate the differentiation degree, calibration degree, fitting degree and clinical effectiveness of the model, respectively. Results　Eight independent variables were screened out by using LASSO regression analysis, which were male, age≥45 years, a history of hypertension, body mass index(BMI)≥25 kg/m², high-density lipoprotein cholesterol(HDL-C), apnea-hypopnea index(AHI)≥30 times/hour, mean arterial oxygen saturation(MSaO₂)≥90%, and four-variable screening tool scores≥10.5 points. The results of multivariate logistic regression analysis showed that male, age≥45 years, a history of hypertension, BMI≥25 kg/m², AHI≥30 times/hour and four-variable screening tool scores≥10.5 points were independent risk factors for OSAHS complicated with CHD(P<0.05). Higher HDL-C level was an independent protective factor for OSAHS complicated with CHD(P<0.05). A Nomogram prediction model for OSAHS complicated with CHD was constructed based on the 7 influencing factors. The ROC curve showed that area under the curve(AUC)(95%CI) of the model in the training set was 0.778(0.747-0.810), with a sensitivity of 71.80%, and a specificity of 71.20%, and AUC(95%CI) of the model in the validation set was 0.797(0.752 - 0.842), with a sensitivity of 78.80%, and a specificity of 70.80%, showing a high differentiation degree. The calibration curve showed that the prediction probability risk of OSAHS complicated with CHD predicted by the model was basically consistent with the actual probability, with a good calibration degree. The results of Hosmer-Lemeshow test(training set: χ²=8.100, P=0.524; validation set: χ²=16.880, P=0.051) proved that the model had a good fitting degree. The decision curve verified the clinical effectiveness of the model. Conclusion　Male, age≥45 years, a history of hypertension, BMI≥25 kg/m², AHI≥30 times/hour, and four-variable screening tool scores≥10.5 points are independent risk factors for OSAHS complicated with CHD. Higher HDL-C level was an independent protective factor for OSAHS complicated with CHD. The Nomogram prediction model constructed based on the 7 influencing factors has better predictive value for OSAHS complicated with CHD.]]> 2025/3/1 12:31:01 YAO Yanli, QIU Xuan, CHEN Yulan, Gulimire Aimaiti, Ayiguzaili Maimaitimin, LIANG Zeyu 111true ［Abstract］　Objective　To explore the correlation of arterial partial pressure of carbon dioxide and blood lactic acid with gastrointestinal bleeding in patients undergoing extracorporeal membrane oxygenation(ECMO). Methods　The clinical data of 94 patients who were admitted to the Emergency Intensive Care Unit of the People′s Hospital of Guangxi Zhuang Autonomous Region for treatment of veno-venous extracorporeal membrane oxygenation(VV-ECMO) or venous-arterial extracorporeal membrane oxygenation(VA-ECMO) from January 2020 to December 2022 were retrospectively collected. The patients were divided into bleeding group(21 cases) and non-bleeding group(73 cases) based on whether they experienced gastrointestinal bleeding during the period of ECMO treatment. The clinical data of arterial partial pressure of carbon dioxide and blood lactic acid were compared between the two groups. The risk factors of gastrointestinal bleeding in the patients undergoing ECMO were analyzed by using binary logistic regression. The predictive efficiency of the relevant indicators for gastrointestinal bleeding was analyzed by using receiver operating characteristic(ROC) curves. Results　Before the treatment of ECMO, there were no significant differences in vasoactive-inotropic score(VIS), mean arterial pressure(MAP), blood lactic acid and arterial partial pressure of carbon dioxide between the two groups(P>0.05). After the treatment of ECMO for 24 hours, VIS, blood lactic acid and arterial partial pressure of carbon dioxide in both groups were lower than those before treatment, and the values of MAP in both groups after the treatment of ECMO for 24 hours were higher than those before treatment, and blood lactic acid and arterial partial pressure of carbon dioxide in the non-bleeding group were lower than those in the bleeding group, and the differences were statistically significant(P<0.05). The results of binary logistic regression analysis showed that arterial partial pressure of carbon dioxide and blood lactic acid 24 hours after the treatment of ECMO were independent risk factors for gastrointestinal bleeding in the patients undergoing ECMO(P<0.05). The results of ROC curve analysis showed that both arterial partial pressure of carbon dioxide and blood lactic acid 24 hours after the treatment of ECMO could effectively predict the occurrence of gastrointestinal bleeding(P<0.05), and the combination of the two indicators could further improve the predictive efficiency［AUC(95%CI)=0.752(0.614-0.890), P<0.05］, and the sensitivity and specificity were 71.43% and 79.45%, respectively. Conclusion　Arterial partial pressure of carbon dioxide and blood lactic acid after the treatment of ECMO for 24 hours can be used as early predictive indicators of gastrointestinal bleeding during the period of ECMO treatment for the patients, which has important clinical significance in reducing the patients′ incidence of gastrointestinal bleeding during the ECMO treatment and improving their survival rate.]]> 2025/3/1 12:31:01 ZHU Liangfeng, SHEN Yin, CHEN Wan, LYU Liwen 110true ［Abstract］　Objective　To observe the clinical efficacy of diquafosol sodium(DQS) combined with fluorometholone in treatment of dry eye syndrome. Methods　A total of 76 dry eye syndrome patients admitted to Department of Ophthalmology, the People′s Hospital of Guangxi Zhuang Autonomous Region in 2023 were recruited and were randomly divided into control group and observation group according to the patient′s visit sequence, with 38 cases in each group. The observation group was treated with DQS combined with fluorometholone, and the control group was treated with sodium hyaluronate combined with fluorometholone. The patients′ right eyes were selected to be observed and analyzed. The ocular surface disease index(OSDI) scores, relevant indicators of tear secretion［results of Schirmer Ⅰ test(SⅠt), tear meniscus height(TMH)］, tear film stability indicators［fluorescein breakup time(FBUT), first noninvasive breakup time(F-NIBUT), and average noninvasive breakup time(A-NIBUT)］, corneal fluorescein staining(CFS) scores, meibomian gland examination indicators(meibomian gland opening status scores, meibomian gland grading scores, meibomian gland secretion trait scores), intraocular pressure, and the occurrence of adverse drug reactions were compared between the two groups before and after treatment. Results　The OSDI scores in both groups after treatment were significantly decreased compared with those before treatment(P<0.05), and the posttherapeutic OSDI scores in the observation group were lower than those in the control group, and the differences were statistically significant(P<0.05). After treatment, the results of SⅠt in the observation group were significantly higher than those in the control group(P<0.05), and there was no significant difference in TMH between the two groups(P>0.05). After treatment, FBUT, F-NIBUT and A-NIBUT in both groups were significantly prolonged compared those before treatment(P<0.05), and the levels of the indicators in the observation group were longer than those in the control group, and the differences were statistically significant(P<0.05). The CFS scores in both groups after treatment were significantly decreased compared with those before treatment(P<0.05), but there were no significant differences in the CFS scores between the two groups(P>0.05). Compared with those before treatment, the meibomian gland opening status scores and meibomian gland secretion trait scores were significantly decreased in both groups after treatment(P<0.05), but the changes in the meibomian gland grading scores were not significant(P>0.05). Before and after treatment, there were no significant differences in the meibomian gland grading scores, meibomian gland opening status scores and meibomian gland secretion trait scores between the two groups(P>0.05). During the treatment period, no adverse events were observed in both groups. There were no significant changes in intraocular pressure between the two groups before and after treatment(P>0.05). Conclusion　Diquafosol sodium combined with fluorometholone can effectively improve the clinical symptoms and signs of the dry eye syndrome patients, and the effects of diquafosol sodium combined with fluorometholone are better than those of sodium hyaluronate combined with fluorometholone, and diquafosol sodium combined with fluorometholone is worthy of clinical application and promotion.]]> 2025/3/1 12:31:01 1,2, JIANG Li², LI Jinfen^2,3, HUANG Hui², LAN Qianqian², QIN Yuanjun², XIONG Jiawei^2,3, LIAO Tao^2,3, XU Fan²]]> LI Yue^1,2, JIANG Li², LI Jinfen^2,3, HUANG Hui², LAN Qianqian², QIN Yuanjun², XIONG Jiawei^2,3, LIAO Tao^2,3, XU Fan² 109true ［Abstract］　Objective　To identify a novel MYO7A gene mutation in a family with late-onset autosomal dominant nonsyndromic sensorineural hearing loss. Methods　The clinical data of a proband and her family members were collected and the family genogram were drawn. Hearing examination was performed on the proband and her family members. Genome whole-exome sequencing was performed on the proband to locate the targeted deafness gene and mutation, and MYO7A gene of the proband and her family members was verified by Sanger sequencing. Results　The proband developed the disease at the age of 16 and was diagnosed with bilateral severe sensorineural hearing loss. There were 18 patients with post-lingual progressive hearing loss in the proband′s family members, characterized by autosomal dominant inheritance. The patients′ age at onset varied among different individuals and differed between families and between generations. The onset age of patients with deafness in generation Ⅳ and generation Ⅴ was significantly younger than that of patients with deafness in generation Ⅲ(P<0.05), showing a tendency of genetic early onset. The average pure-tone audiometric threshold in both ears of the proband was 83 dBHL(air conduction), and the maximum intensity of bone conduction in each frequency band showed no response, with more pronounced losses in the mid-to-high frequency ranges. A heterozygous mutation of c.689C>T in exon 7 of MYO7A gene was located by using whole-exome sequencing. Sanger sequencing confirmed that the mutation was detected in the proband and the deaf family members of the proband. Conclusion　This paper reports a case of hereditary non-syndromic hearing loss caused by the heterozygous mutation of c.689C>T in exon 7 of MYO7A gene in a pedigree and discovers a unique genetic early onset phenomenon of this mutation in the family genogram in China.]]> 2025/3/1 0:00:00 ZUO Yangjin, WEN Chunxiu, HE Sheng, QIN Zailong, YANG Qi, YI Shang, CHEN Biyan 108true ［Abstract］　Objective　To analyze the value of melatonin-to-procalcitonin ratio(MLT/PCT) in predicting the risk of acute kidney injury(AKI) and its association with short-term survival in sepsis patients. Methods　A total of 110 patients with sepsis who were admitted to Chongqin Jiangjin Second People′s Hospital from January 2021 to December 2023 were recruited. The patients were divided into AKI group(49 cases) and non-AKI group(61 cases) according to the occurrence of AKI. The patients were divided into survival group(74 cases) and death group(36 cases) according to their survival status within 28 days. On the second day after admission, the serum procalcitonin(PCT) levels were detected by using electrochemiluminescence immunoassay, and the serum melatonin(MLT) levels were detected by using enzyme-linked immunosorbent assay(ELISA), and MLT/PCT was calculated. Multivariate logistic regression was used to analyze the influencing factors of AKI in patients with sepsis. The predictive efficacy of MLT, PCT and MLT/PCT for AKI in the patients with sepsis was analyzed by using receiver operating characteristic(ROC) curve. Kaplan-Meier method was used to analyze the association between MLT/PCT and the patients′ survival prognosis. Results　The patients in the AKI group were older than those in the non-AKI group, and the Acute Physiology and Chronic Health Evaluation Ⅱ(APACHE Ⅱ) scores, proportion of hypertensive patients, white blood cell count, neutrophil percentage, alanine aminotransferase, aspartate aminotransferase, total bilirubin, direct bilirubin, serum creatinine, urea nitrogen, blood lactate and PCT levels in the AKI group were higher than those in the non-AKI group, and the percentage of lymphocytes, red blood cell count, platelet count, blood pH, MLT levels and MLT/PCT in the AKI group were lower than those in the non-AKI group, and the differences were statistically significant(P<0.05). The results of ROC curve analysis showed that MLT/PCT［AUC(95%CI)=0.920(0.853-0.963)］ was superior to MLT and PCT(P<0.05) in predicting AKI occurrence in the sepsis patients, with sensitivity of 93.92% and specificity of 75.38%. The results of multivariate logistic regression analysis showed that higher MLT/PCT was an independent protective factor for AKI in sepsis patients(P<0.05). The MLT levels and MLT/PCT in the survival group were significantly higher than those in the death group(P<0.05). The survival prognosis of the high MLT/PCT group(≥3.3, 55 cases) was better than that of the low MLT/PCT group(<3.3, 55 cases)(log-rank test: χ²=23.627, P<0.001). Conclusion　MLT/PCT can be used as an indicator to predict the risk of AKI in sepsis patients, and MLT/PCT is associated with short-term survival prognosis of the sepsis patients.]]> 2025/3/1 12:31:01 YUAN Yuan, HUANG Peiyong, LIU Shulin, YUAN Yuting 107true ［Abstract］　Objective　To observe radioanatomical indicators of the right atrial appendage in patients with brady sinus arrhythmia based on cardiac computed tomography(CT) angiography. Methods　A total of 74 patients with brady sinus arrhythmia who underwent pacemaker implantation in the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2017 to May 2018 were recruited as case group. Other 20 individuals without typical cardiovascular disease symptoms who received physical examination during the same period and 26 patients who suffered from other systemic diseases and needed cardiac CT screening to exclude cardiac risk were selected as control group(46 cases). The control group had normal electrocardiogram, with no obvious structural or functional changes in cardiac ultrasound examination. Cardiac 128-row multidetector CT angiography was performed in both groups. Philips IntelliSpace Portal Workstation software was used to measure the axial angle, sagittal angle, coronal distance, oblique vertical diameter, transverse diameter, circumference and other indicators of the right atrial appendage. Results　In the control group, the axial angle of the right atrial appendage ranged from 0.40° to 11.91°, with an average of (6.56±3.17)°, and the sagittal angle of the right atrial appendage ranged from 40.03° to 60.58°, with an average of (50.37±5.21)°, and the coronal distance of the right atrial appendage ranged from 4.75 cm to 8.89 cm, with an average of (6.96±1.08)cm, and the oblique vertical diameter of the right atrial appendage ranged from 2.61 cm to 5.07 cm, with an average of (3.84±0.68)cm, and the transverse diameter of the right atrial appendage ranged from 1.87 cm to 4.18 cm, with an average of (3.01±0.59)cm, and the circumference of the right atrial appendage ranged from 10.31 cm to 14.27 cm, with an average of (12.38±0.92)cm. The axial angle of the right atrial appendage in the case group was greater than that in the control group, and the sagittal angle of the right atrial appendage in the case group was smaller than that in the control group, and the differences were statistically significant(P<0.05), but there were no statistically significant differences in the other radioanatomical indicators of the right atrial appendage between the two group(P>0.05). In the males, the axial angle of the right atrial appendage in the case group was greater than that in the control group, and the sagittal angle of the right atrial appendage in the case group was smaller than that in the control group, and the differences were statistically significant(P<0.05). In the females, the axial angle of the right atrial appendage in the case group was greater than that in the control group, and the sagittal angle and the oblique vertical diameter of the right atrial appendage in the case group were smaller than those in the control group, and the differences were statistically significant(P<0.05). In the subjects <60 years, the axial angle of the right atrial appendage in the case group was greater than that in the control group, and the sagittal angle of the right atrial appendage in the case group was smaller than that in the control group, and the differences were statistically significant(P<0.05). In the subjects ≥60 years, the sagittal angle of the right atrial appendage in the case group was smaller than that in the control group, and the difference was statistically significant(P<0.05). Conclusion　The anatomical morphology of the right atrial appendage can be observed objectively by using cardiac CT angiography, which has guiding significance for pacemaker implantation in clinical practice.]]> 2025/3/1 0:00:00 1, ZHAO Chunli², DENG Jinlong¹, WEI Youyong², WU Dongfeng¹, GUAN Xiaohui², WU Yinxiong¹]]> LI Weijun¹, ZHAO Chunli², DENG Jinlong¹, WEI Youyong², WU Dongfeng¹, GUAN Xiaohui², WU Yinxiong¹ 106true ［Abstract］　Objective　To analyze the correlation between methylenetetrahydrofolate reductase(MTHFR) and protein C(PROC) gene polymorphisms and the occurrence of venous thromboembolism(VTE) in patients with malignant tumors. Methods　The clinical data of 227 patients with malignant tumors who were admitted to Affiliated Cancer Hospital of Xinjiang Medical University from November 14, 2023 to February 14, 2024 were retrospectively analyzed. According to the occurrence of VTE, the patients were divided into VTE group(47 cases) and non-VTE group(180 cases). The polymorphisms of PROC gene(rs199469469 locus) and MTHFR gene(rs1801133 locus) and the other clinical data were compared between the two groups. Multivariate logistic regression was used to analyze the factors affecting the occurrence of VTE in patients with malignant tumors. Results　The level of D-dimer and the proportion of the patients with a history of diabetes in the VTE group were higher than those in the non-VTE group, and the differences were statistically significant(P<0.05). There was a significant difference in the genotype distribution of rs1801133 locus of MTHFR gene between the two groups(P<0.05). The frequency of A allele of rs1801133 locus of MTHFR gene in the VTE group was significantly higher than that in the non-VTE group［53.19%(50/94)vs 38.89%(140/360), χ²=3.945, P=0.047］. There was no significant difference in the genotype of rs199469469 locus of PROC gene between the two groups(P>0.05). The results of multivariate logistic regression analysis showed that higher D-dimer level［OR(95%CI)=1.436(1.213-1.696)］, a history of diabetes［OR(95%CI)=2.318(1.125-6.808)］, AA genotype of rs1801133 locus of MTHFR gene［OR(95%CI)=1.927(1.459-3.751)］ were independent risk factors for promoting the occurrence of VTE in patients with malignant tumors(P<0.05). Conclusion　The polymorphisms of rs1801133 locus of MTHFR gene are correlated with the occurrence of VTE in malignant tumor patients, but the polymorphisms of rs199469469 locus of PROC gene are not significantly correlated with the occurrence of VTE.]]> 2025/2/7 8:31:46 1, NURBIA Rexiti¹, HE Man², ZHU Zihui², HOU Xueping², WANG Yuying², JIANG Weihua²]]> CUI Yingxue¹, NURBIA Rexiti¹, HE Man², ZHU Zihui², HOU Xueping², WANG Yuying², JIANG Weihua² 105true ［Abstract］　Objective　To explore the effects of mechano-growth factor(MGF) on proliferation, migration and differentiation of cartilage endplate-derived stem cells(CESCs) and their acting mechanisms. Methods　The patients with degenerative disc disease who underwent disc fusion surgery in the People′s Hospital of Guangxi Zhuang Autonomous Region were recruited. During the surgery, about 2 cm³ of the patients′ cartilage endplate tissues of intervertebral disc were collected, and CESCs were collected and cultured. The effects of MGF on the proliferation of CESCs were evaluated by Cell Counting Kit-8(CCK-8) assay. The effects of MGF on the migration and invasion abilities of CESCs were evaluated by Transwell experiment. The expression levels of osteogenic, chondrogenic and lipogenic differentiation-related factors were detected by using reverse transcription-polymerase chain reaction(RT-PCR) and Western blot assay, and the influence of phosphorylated extracellular signal-regulated kinase(ERK) on the acting effect of MGF was analyzed. Results　The results of CCK-8 assay showed that MGF could promote the proliferation of CESCs in a dose-dependent manner. The results of Transwell experiment showed that MGF could promote the migration of CESCs. After the intervention of ERK inhibitor PD98059, the proliferative effect of MGF was significantly weakened(P<0.05). After the intervention of insulin-like growth factor-1 receptor(IGF-1R) inhibitor PQ401, the promoting migration effect of MGF was also significantly weakened(P<0.05). The results of RT-PCR detection showed that the mRNA expression levels of alkaline phosphatase(ALP), Runt-related transcription factor 2(Runx2) and osteocalcin(OC) in the MGF group were significantly lower than those in the control group(P<0.05), while the mRNA expression levels of aggrecan(AGG), SRY-related high-mobility group box gene-9(SOX-9) and type Ⅱ collagen(CⅡ) in the MGF group were significantly higher than those in the control group(P<0.05). The results of Western blot assay also showed that the protein expression levels of ALP, Runx2 and OC in the MGF group were significantly lower than those in the control group(P<0.05), while the protein expression levels of AGG, SOX-9 and CⅡ in the MGF group were significantly higher than those in the control group(P<0.05). The ratio of phosphorylated ERK(p-ERK) to total extracellular signal-regulated kinase(t-ERK) in the MGF group was significantly higher than that in the control group(P<0.05), and the ratio of p-ERK to t-ERK in the MGF+PQ401 group was lower than that in the MGF group, but the ratio of p-ERK to t-ERK in the MGF+PQ401 group was still higher than that in the control group, and the differences were statistically significant(P<0.05). Conclusion　MGF promotes the proliferation and migration of CESCs by inducing ERK phosphorylation.]]> 2025/2/7 8:31:46 LI Haoxi, WU Chunlan, PENG Guanlu, MO Kaiqi, YAO Shuyu, YU Chengqiang, LI Zhuhai, WEI Jianxun 104true ［Abstract］　Objective　To analyze the expressions of signal peptide-CUB-EGF-like domain-containing protein 1(SCUBE1) and integrin αvβ3 in the serum of patients with coronary heart disease(CHD), and to analyze the correlation between these expressions and sensitivity to clopidogrel after percutaneous coronary intervention(PCI) surgery. Methods　A total of 164 CHD patients admitted to Sanya Central Hospital from March 2022 to September 2023 were recruited as CHD group, and 150 healthy individuals undergoing physical examination during the same period were selected as control group. According to inhibition rate of platelet aggregation(IPA), the CHD patients were divided into low-sensitive group(41 cases, IPA<20%) and high-sensitive group(123 cases, IPA≥20%). Enzyme-linked immunosorbent assay(ELISA) was used to detect serum SCUBE1 and αvβ3 levels in the control group on the day of physical examination and in the CHD group before PCI surgery, immediately after PCI surgery, and 48 hours after PCI surgery. Multivariate logistic regression was used to analyze the factors affecting the decrease of clopidogrel sensitivity in the CHD patients after PCI surgery. Receiver operating characteristic(ROC) curve was used to analyze the predictive efficiency of preoperative serum SCUBE1 and αvβ3 levels for the decrease of clopidogrel sensitivity in the CHD patients after PCI surgery. Results　The serum SCUBE1 and αvβ3 levels before PCI surgery, immediately after PCI surgery, and 48 hours after PCI surgery in the CHD group were significantly higher than those in the control group(P<0.05), and the postoperative serum SCUBE1 and αvβ3 levels showed an upward trend, and the differences in intra-group comparisons between different time points were statistically significant(P<0.05). The serum SCUBE1 and αvβ3 levels before PCI surgery, immediately after PCI surgery, and 48 hours after PCI surgery in the low-sensitive group were higher than those in the high-sensitive group, and the differences were statistically significant between the two groups(P<0.05). The results of multivariate logistic regression analysis showed that preoperative higher levels of platelet(PLT)［OR(95%CI)=2.693(1.182-6.134)］, mean platelet volume(MPV)［OR(95%CI)=2.285(1.328-3.933)］, SCUBE1［OR(95%CI)=2.571(1.352-4.890)］, and αvβ3［OR(95%CI)=3.254(1.113-7.340)］ were independent risk factors for promoting the decrease in sensitivity to clopidogrel after PCI surgery(P<0.05). The results of ROC curve analysis showed that both serum SCUBE1 and αvβ3 levels before surgery could effectively predict the decrease of clopicogrel sensitivity in the CHD patients after PCI surgery, and the combined predictive efficiency of the two indicators was higher［AUC(95%CI)=0.899(0.842-0.940)］, with sensitivity of 72.61% and specificity of 94.31%. Conclusion　The serum SCUBE1 and αvβ3 levels of CHD patients increase, and preoperative serum SCUBE1 and αvβ3 levels can effectively predict the decrease of clopidogrel sensitivity after PCI surgery, which is worth the attention of clinicians.]]> 2025/2/7 8:31:46 1, WANG Xiaoli¹, WU Shuhua², YUAN Yongcheng³, QIN Jiangjun⁴]]> CHEN Juan¹, WANG Xiaoli¹, WU Shuhua², YUAN Yongcheng³, QIN Jiangjun⁴ 103true ［Abstract］　Objective　To analyze the bone mineral density characteristics and the risk factors of concurrent frailty in elderly patients with hip fractures. Methods　The clinical data of 267 elderly patients with hip fractures who were admitted to Beijing Jishuitan Hospital, Captial Medical University from September 2021 to November 2021 were retrospectively analyzed, and according to the evaluation results of the 5-item FRAIL scale, the patients were divided into frail group(with concurrent frailty, 110 cases) and non-frail group(without concurrent frailty, 157 cases). The results of hip joint volumetric bone mineral density(vBMD) measured by quantitative computed tomography(QCT) were compared between the two groups, as well as the clinical data including demographic characteristics, Charlson comorbidity index(CCI) scores, calf circumference, fibrinogen-to-prealbumin ratio(FPR), biochemical markers of bone metabolism and risk of sarcopenia. Multivariate logistic regression was used to analyze the factors influencing concurrent frailty in the elderly patients with hip fractures. Results　The frail group was older than the non-frail group, and the levels of FDR beta-isomerized C-terminal telopeptide of type Ⅰ collagen(β-CTX), total procollagen type Ⅰ N-terminal propeptide(tP1NP), as well as the proportion of the patients with nutritional risk, risk of sarcopenia and CCI scores>2 points in the frail group were higher than those in the non-frail group, and the levels of body mass index(BMI), free triiodothyronine(FT₃), calf circumference, trabecula vBMD of the total hip bone(THT vBMD), trabecula vBMD of the femoral neck(FNT vBMD), trabecula vBMD of the intertrochanter(ITT vBMD) and serum 25-(OH)D₃ in the frail group were lower than those in the non-frail group, and the differences were statistically significant(P<0.05). The results of stratified analysis showed that in the female patients, the levels of THT vBMD and FNT vBMD in the frail group were significantly lower than those in the non-frail group(P<0.05), and in the male patients, only THT vBMD levels in the frail group were significantly lower than those in the non-frail group(P<0.05). The results of multivariate logistic regression analysis showed that older age, elevated levels of β-CTX and FPR, as well as nutritional risk and CCI scores greater than 2 points were independent risk factors for promoting the occurrence of frailty, while elevated levels of calf circumference, THT vBMD, and 25-(OH)D₃ were protective factors for inhibiting the occurrence of frailty(P<0.05). Conclusion　Hip bone mineral density in elderly patients with hip fractures and concurrent frailty is characterized by reduced THT vBMD, FNT vBMD, and ITT vBMD. In addition to the factors of age, nutritional status, calf circumference and comorbidities, biochemical markers of bone metabolism β-CTX and 25-(OH)D₃ are also significantly associated with frailty. Clinicians should pay attention to these factors and conduct stratified management as soon as possible to improve the prognosis of the patients.]]> 2025/2/7 8:31:46 1, YU Xiaohong², MA Kangkang³]]> CHEN Zhifang¹, YU Xiaohong², MA Kangkang³ 102true ［Abstract］　Objective　To construct a prognostic risk model for lung adenocarcinoma based on cuproptosis-related long non-coding RNAs(lncRNAs) signature and to verify the model by experiment. Methods　The lung adenocarcinoma data were obtained from The Cancer Genome Atlas(TCGA) database to construct the prognostic risk model of cuproptosis-related lncRNAs signature(CRLS) and risk scores were calculated, and the patients were divided into high-risk group(≥the median of risk scores) and low-risk group(Results　Seven CRLS were finally screened to be significantly associated with overall survival(OS), with risk scores=-0.308 1×AC090948.1 expression+0.460 4×CASC15 expression-0.417 0×AL353804.1 expression-1.432 2×AP000302.1 expression+0.441 7×AC026356.1 expression-0.619 0×AC007613.1 expression+0.271 0×AL161431.1 expression. As the risk scores increased, the number of patients who died increased. The constructed risk model was able to effectively differentiate between low-risk patients and high-risk patients, and the survival prognosis of the low-risk group was better than that of the high-risk group(log-rank test: χ²=29.352, P<0.001). The conformity index of the risk scores was higher than that of the age, gender, and clinical stage, and the predictive accuracy of the risk scores was better. The IC₅₀ values of cisplatin, paclitaxel, docetaxel, 5-fluorouracil, vinorelbine, camptothecin, gemcitabine, gefitinib and ceritinib in the high-risk group were lower than those in the low-risk group, and the IC₅₀ values of erlotinib and rapamycin in the high-risk group were higher than those in the low-risk group, and the differences were statistically significant(P<0.05). The TIDE scores of the low-risk group were lower than those of the high-risk group, and the differences were statistically significant(P<0.05). Cellular experiments showed that the cell proliferation rate, the clone formation rate and cell invasion rate in the CASC15 group were significantly increased compared with those in the blank group and the control group(P<0.05). Conclusion　The risk model consisting of 7 CRLS can effectively predict the survival prognosis of lung adenocarcinoma patients, and risk score of the model can be used as an independent prognostic factor. The model provides a new theoretical basis for lung adenocarcinoma patients to choose conventional drugs or immunotherapy.]]> 2025/2/7 8:31:46 1, ZHEN Hua¹, YANG Lei¹, GUO Jingjing²]]> JIAN Ruonan¹, ZHEN Hua¹, YANG Lei¹, GUO Jingjing² 101true ［Abstract］　Objective　To analyze the correlation of days of sexual abstinence with semen quality and sperm function indicators in patients with asthenozoospermia. Methods　A total of 192 patients with asthenozoospermia who were admitted to Reproductive Medicine and Genetic Center, the People′s Hospital of Guangxi Zhuang Autonomous Region from October 28, 2022 to June 15, 2023 were recruited and divided into three groups according to the days of sexual abstinence before semen collection: group A(days of sexual abstinence ≤2 days, 41 cases), group B(days of sexual abstinence of 3-4 days, 114 cases) and group C(days of sexual abstinence ≥5 days, 37 cases). Fresh semen samples were collected via masturbation and were optimized using density gradient centrifugation method combined with swim-up method. The semen quality, sperm morphology and sperm DNA fragmentation rate(SDF) were compared among the three groups before and after optimization of the fresh semen samples. The correlation between the days of sexual abstinence and the indicators related to semen was analyzed. Results　Before semen optimization, there were significant differences among the three groups in terms of semen volume, total sperm count, total progressively motile sperm count and SDF(P<0.05), and group A had significantly lower semen volume, total sperm count, total progressively motile sperm count and SDF than group B and group C(P<0.05), and group B had significantly lower SDF than group C(P<0.05). After semen optimization, there was significant difference in SDF among the three groups(P<0.05), and group A had significantly lower SDF than group B and group C(P<0.05). Before semen optimization, days of sexual abstinence were positively correlated with semen volume(r=0.225, P=0.002), total sperm count(r=0.200, P=0.016) and SDF(r=0.226, P=0.006), and were not significantly correlated with sperm concentration, total sperm motility rate, progressive motility percentage, total progressively motile sperm count and the percentage of normal sperm morphology(P>0.05). After semen optimization, days of sexual abstinence were positively correlated with SDF(r=0.171, P=0.036), and were not significantly correlated with the other indicators related to semen(P>0.05). Conclusion　Days of sexual abstinence in the asthenozoospermia patients are positively correlated with semen volume, total sperm count and SDF. Shortening the days of sexual abstinence in the infertile men can help to reduce their SDF.]]> 2025/2/7 8:31:46 1, LIU Haifang¹, CHEN Liangshi², ZHANG Xiaohui², HUANG Yueyue², SHI Wen², WEI Pingpin², LI Zhengda², MAO Xianbao², WANG Shikai², XUE Lintao^1,2]]> LI Yaoxuan¹, LIU Haifang¹, CHEN Liangshi², ZHANG Xiaohui², HUANG Yueyue², SHI Wen², WEI Pingpin², LI Zhengda², MAO Xianbao², WANG Shikai², XUE Lintao^1,2 100true ［Abstract］　Objective　To analyze the therapeutic effect of ultrasound-guided pulsed radiofrequency of cervical dorsal rami nerve combined with cervical shoulder muscle on neck and shoulder pain deriving from cervical dorsal rami nerve. Methods　A total of 72 patients who were diagnosed with neck and shoulder pain deriving from cervical dorsal rami nerve in Department of Pain Management, Wuhan Hankou Hospital from January 2019 to October 2021 were recruited. The patients were divided into observation group(receiving therapy of ultrasound-guided pulsed radiofrequency of cervical dorsal rami nerve combined with cervical shoulder muscle) and control group(receiving therapy of ultrasound-guided pulsed radiofrequency of cervical dorsal rami nerve) by using random number table method, with 36 cases in each group. The Visual Analogue Scale(VAS) scores and Neck Disability Index(NDI) of the patients were assessed before treatment, and 1 day, 1 week, 1 month, 3 months, and 6 months after treatment. The therapeutic effect and incidence of complications were assessed 6 months after treatment. The patients were followed up through their further consultations with their doctors in the outpatient department or phone calls after discharge. Results　At different time points after treatment, the VAS scores and NDI in the two groups were lower than those before treatment. At 3 and 6 months after treatment, the VAS scores and NDI in the observation group were lower than those in the control group, and the differences were statistically significant(P<0. 05). There were no statistically significant differences in VAS scores and NDI between the two groups before treatment, 1 day after treatment, 1 week after treatment, and 1 month after treatment(P>0. 05). At 6 months after treatment, the significantly effective rate of the observation group was higher than that of the control group, and the difference was statistically significant(P<0.05). After treatment, there were no complications such as bleeding, infection and nerve damage at the puncture sites in both groups. All the patients were followed up for more than 6 months, and the longest follow-up period was 1 year. At the last follow-up, both mild relief and ineffective patients underwent the therapy of pulsed radiofrequency of cervical dorsal rami nerve combined with cervical shoulder muscle, and after treatment for 1 time, VAS scores decreased by more than 50% compared with those before treatment. Conclusion　Ultrasound-guided pulsed radiofrequency of cervical dorsal rami nerve combined with cervical shoulder muscle can significantly improve pain and cervical function in patients with neck and shoulder pain deriving from cervical dorsal rami nerve, and the therapy is safe and effective.]]> 2025/2/7 0:00:00 1, Shi Huawu², GAO Song¹, ZHOU Mi¹, ZHANG Yan¹, YU Hui¹, XIANG Jing¹, LI Jianjun¹, ZHOU Weimin¹]]> YANG Fan¹, Shi Huawu², GAO Song¹, ZHOU Mi¹, ZHANG Yan¹, YU Hui¹, XIANG Jing¹, LI Jianjun¹, ZHOU Weimin¹ 99true ［Abstract］　Objective　To analyze characteristics of the allelic distribution and haplotype polymorphism of HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DQB1 in the renal transplantation recipients of a single-center Han population in Shandong, and to obtain human leukocyte antigen(HLA) genetic parameters of the kidney transplant population. Methods　A total of 1 470 renal transplantation recipients who underwent allele detection of HLA-A, HLA-B, HLA-C, HLA-DRB1 and HLA-DQB1 in the Affiliated Hospital of Qingdao University from June 2019 to December 2022 were selected. The method of polymerase chain reaction-sequence specific oligonucleotide probe(PCR-SSOP) was used for allele detection of HLA. The frequency of HLA alleles was calculated by using direct counting method, and Hardy-Weinberg equilibrium test was performed for the loci of each allele. The common haplotype frequencies were calculated by using Arlequin 3.5.2.2 software. Results　A total of 216 alleles were detected in the 1 470 patients, among which 36, 86, 33, 40 and 21 alleles were detected in HLA-A, HLA-B, HLA-C, HLA-DRB1 and HLA-DQB1, respectively. There were no significant differences between the observed values of HLA-A, HLA-B and HLA-C alleles and the expected values of HLA-A, HLA-B and HLA-C alleles(P>0.05). There were significant differences between the observed values of HLA-DRB1 and HLA-DQB1 alleles and the expected values of HLA-DRB1 and HLA-DQB1 alleles(P<0.05). There were a total of 452 haplotype pairs of HLA-A-HLA-B, 219 haplotype pairs of HLA-B-HLA-C, 666 haplotype pairs of HLA-A-HLA-B-HLA-C, 551 haplotype pairs of HLA-B-HLA-DRB1, 91 haplotype pairs of HLA-DRB1-HLA-DQB1, 1 367 haplotype pairs of HLA-A-HLA-B-HLA-C-HLA-DRB1, and 1 434 haplotype pairs of HLA-A-HLA-B-HLA-C-HLA-DRB1-HLA-DQB1. HLA-A^*30:01-HLA-B^*13:02-HLA-C^*06:02-HLA-DRB1^*07:01-HLA-DQB1^*02:02 haplotype frequency was the highest. Conclusion　HLA-A^*30:01-HLA-B^*13:02-HLA-C^*06:02-HLA-DRB1^*07:01-HLA-DQB1^*02:02 haplotype frequency is the highest in the renal transplantation recipients of the Han population in Shandong. This study provides a reference for donor allele search and recipient population genetics research.]]> 2025/2/7 8:31:46 1, KONG Yirong², WANG Liqin¹, WANG Rong¹, JU Qiang¹, CHEN Shumin¹, WANG Haiyan¹]]> SHAO Changfeng¹, KONG Yirong², WANG Liqin¹, WANG Rong¹, JU Qiang¹, CHEN Shumin¹, WANG Haiyan¹ 98true ［Abstract］　Objective　To analyze the changes in the types of diseases of children and adolescents with psychiatric and psychological disorders in the outpatient clinic of a psychiatric hospital in Nanning Guangxi from 2014 to 2023. Methods　The clinical data of 37 177 children and adolescents who first visited their doctors in the outpatient department of Nanning Fifth People′s Hospital from January 2014 to December 2023 were retrospectively collected. The patients′ disease diagnoses were classified by reference to International Classification of Diseases, 10th Revision(ICD-10). The annual composition ratio of initial diagnosis types was statistically analyzed, and the average growth rate of the composition ratio of the types was calculated, and the changing trends were analyzed using chi-square test for trend. Results　From 2014 to 2023, the average annual composition ratios of the initial diagnosis types from highest to lowest were F30-F39(27.93%), consultation(23.56%), F20-F29(12.07%), F40-F48(9.88%), F00-F09(9.80%), F90-F98(6.37%), other types(5.67%), visits for mental health problems(2.07%), F70-F79(1.41%) and F50-F59(1.24%). The composition ratios of F00-F09, F20-F29, F50-F59, F70-F79, other types and consultation in the patients visiting their doctors showed a decreasing trend, and the average decline rates of the composition ratios were 7.20%, 49.06%, 7.41%, 25.98%, 7.41% and 4.80%, respectively. The composition ratios of F30-F39, F40-F48 and visits for mental health problems showed an increasing trend, and the average growth rates of the composition ratios were 26.95%, 15.97% and 51.12%, respectively. The results of chi-square test for trend showed that the differences were statistically significant(P<0.05). In the F30-F39 disease category, the average annual composition ratios from highest to lowest were as follow: depressive episodes(80.12%), other types of mood ［affective］ disorders(12.64%), bipolar disorders(5.18%) and manic episodes(2.06%). The composition ratios of manic episodes and bipolar disorders showed a declining tendency, and their average declining rates of the composition ratio were 10.49% and 17.99%, respectively. In contrast, the composition ratios of depressive episodes and other types of mood ［affective］ disorders showed an upward trend, and their average growth rates of the composition ratio were 32.08% and 16.97%, respectively. The results of chi-square test for trend showed that the differences were statistically significant(P<0.05). Conclusion　From 2014 to 2023, the composition ratios of visits for mental health problems and F30-F39 type disease in the children and adolescents consulting doctors in the outpatient department of Nanning Fifth People′s Hospital increase rapidly, while the composition ratios of traditional mental diseases such as schizophrenia decrease. In the future, psychological health services should be strengthened to handle problems such as internalization of emotions in the children and adolescents.]]> 2025/2/7 8:31:46 1, ZHENG Dandan¹, NONG Yuxian¹, HUANG Shujin¹, LYU Xiaoping¹, LONG Jianxiong², FAN Xiying¹, SONG Li¹, LI Jinxiu¹]]> FANG Hui¹, ZHENG Dandan¹, NONG Yuxian¹, HUANG Shujin¹, LYU Xiaoping¹, LONG Jianxiong², FAN Xiying¹, SONG Li¹, LI Jinxiu¹ 97true ［Abstract］　Objective　To analyze the deaths of children under 5 years old with congenital heart disease in Guangxi from 2017 to 2022. Methods　The data from the death report cards of 1 271 cases of children under 5 years old who died of congenital heart disease in 111 counties(cities and districts) of Guangxi from January 2017 to December 2022 were downloaded from Guangxi Women and Children Health Service Information Management System and were analyzed. Results　From 2017 to 2022, the annual mortality of congenital heart disease in children under 5 years old in Guangxi decreased from 40.37/100 000 to 24.60/100 000, and the mortality of children in urban and rural areas was 21.58/100 000 and 44.78/100 000, respectively. The majority of the deaths of children under 5 years old with congenital heart disease were in hospitals(47.92%), and most of them received hospital treatment before death(73.96%), and provincial(city) hospitals(60.50%) were the most important diagnosis and treatment institutions. Conclusion　The annual mortality of children under 5 years old with congenital heart disease in Guangxi showed a downward trend from 2017 to 2022, and the mortality of rural children was higher than that of urban children. It is necessary to continuously improve the quality of primary pediatric medical services and accelerate the balanced distribution of high-quality medical resources.]]> 2025/2/7 0:00:00 ZHANG Yuxing, WEI Ping, WEI Jishan, HUANG Xuhua, LIU Xianghong 96true ［Abstract］　Objective　To study the regulatory role of NOD-like receptor family pyrin domain containing 3(NLRP3) inflammasome in acute kidney injury(AKI) mediated by high-mobility group nucleosome-binding protein 1(HMGN1) and its downstream Toll-like receptor 4(TLR4)-nuclear factor-kappa B(NF-κB) signaling pathways. Methods　Eighteen C57BL/6 mice were selected and randomly divided into control group, AKI group and cytokine release inhibitory drug 3(CRID3) group(AKI+NLRP inhibitor), with 6 mice in each group. A mouse model of AKI induced by ischemia-reperfusion(I/R) was established by using the bilateral renal pedicle clamping method. The changes in renal function were evaluated by detecting the levels of serum blood urea nitrogen(BUN), serum creatinine(Scr) and cystatin C. The pathological changes in renal tissues were observed by using hematoxylin-eosin(HE) staining, Masson staining and Sirius Red staining. The expressions of HMGN1, NLRP3 and TLR4 in the renal tissues were detected by using immunohistochemical staining and Western blot method. The levels of inflammatory factors of interleukin-1(IL-1), interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) in serum were detected by using enzyme-linked immunosorbent assay(ELISA). Results　Compared with those in the control group, the levels of serum BUN, Scr and cystatin C were significantly increased(P<0.05), and the pathological injuries of the renal tissues were aggravated, with renal tubular necrosis, interstitial fibrosis and inflammatory cell infiltration, and the expressions of HMGN1, NLRP3 and TLR4 proteins in the renal tissues were significantly increased(P<0.05), and the levels of serum IL-1, IL-6 and TNF-α were significantly increased in the AKI group(P<0.05). Compared with those in the AKI group, the levels of serum BUN, Scr and cystatin C were significantly decreased(P<0.05), and the pathological injuries, inflammation and fibrosis of the renal tissues were alleviated, and the expressions of HMGN1, NLRP3 and TLR4 proteins in the renal tissues were significantly decreased(P<0.05), and the levels of serum IL-1, IL-6 and TNF-α were significantly decreased in the CRID3 group(P<0.05). Conclusion　NLRP3 participates in the pathological process of AKI by regulating the expression of HMGN1 and the downstream TLR4 signaling pathway. Inhibiting the expression of NLRP3 can improve renal function, alleviate pathological injuries and inflammatory response in renal tissues.]]> 2025/12/31 13:19:40 1, SHAO Xingqin¹, ZHANG Lang¹, XIE Ying², YUAN Jing², ZHA Yan², LIN Xin²]]> LUO Yuhan¹, SHAO Xingqin¹, ZHANG Lang¹, XIE Ying², YUAN Jing², ZHA Yan², LIN Xin² 95true ［Abstract］　Objective　To explore correlations of serum microRNA-27b-3p(miR-27b-3p) and microRNA-142-5p(miR-142-5p) levels with disease progression of clear cell renal cell carcinoma(ccRCC) and poor prognosis after retroperitoneal laparoscopic partial nephrectomy(RLPN). Methods　A total of 110 patients with ccRCC who underwent RLPN in the Second Hospital of Qinhuangdao from January 2020 to February 2022 were recruited as ccRCC group, and 55 healthy individuals who underwent physical examination during the same period were selected as control group. The levels of serum miR-27b-3p and miR-142-5p in the ccRCC group before the operation and those in the control group during physical examination were detected by using real-time fluorescence quantitative polymerase chain reaction. The correlations of serum miR-27b-3p and miR-142-5p levels with the clinicopathological indicators of ccRCC were analyzed. Cox regression was used to analyze the factors influencing poor prognosis of the ccRCC patients after RLPN. The receiver operating characteristic(ROC) curve was used to analyze the efficacy of serum miR-27b-3p and miR-142-5p levels in predicting poor prognosis of the ccRCC patients after RLPN. The progression-free survival(PFS) curves of the ccRCC patients with different levels of serum miR-27b-3p and miR-142-5p after RLPN were plotted by K-M method and were compared by using log-rank test. Results　Compared with the control group, the ccRCC group had lower serum level of miR-27b-3p, but higher serum level of miR-142-5p, with statistically significant differences between the two groups(P<0.05). The levels of serum miR-27b-3p and miR-142-5p were significantly correlated with TNM staging and Fuhrman grading(P<0.05), but were not significantly correlated with gender, age, and tumor site(P>0.05). The poor prognosis rate of the ccRCC patients after RLPN was 23.64%(26/110). The results of multivariate Cox regression analysis showed that TNM stage T₂, Fuhrman grades Ⅲ-Ⅳ, and higher serum miR-142-5p level were independent risk factors for poor prognosis in the ccRCC patients after RLPN(P<0.05), while higher serum miR-27b-3p level was an independent protective factor against poor prognosis in the ccRCC patients after RLPN(P<0.05). The results of ROC curve analysis showed that the efficacy of the combined use of serum miR-27b-3p and miR-142-5p in predicting poor prognosis of the ccRCC patients after RLPN was better than that of a single indicator(Z_{combination of the two indicators-miR-27b-3p}=2.148, P=0.032; Z_{combination of the two indicators-miR-142-5p}=2.329, P=0.020), with a sensitivity of 0.885 and a specificity of 0.857. The progression-free survival prognosis of the high miR-27b-3p group(≥1.37, 53 cases) was significantly better than that of the low miR-27b-3p group(<1.37, 57 cases)(P<0.05). The progression-free survival prognosis of the low miR-142-5p group(<1.08, 58 cases) was significantly better than that of the high miR-142-5p group(≥1.08, 52 cases)(P<0.05). Conclusion　The levels of serum miR-27b-3p and miR-142-5p in ccRCC patients are related to the disease progression and prognosis after RLPN. The combined detection of the two indicators is helpful for the early identification of high-risk patients with poor prognosis after RLPN.]]> 2025/12/31 13:19:40 ZHANG Yuan, YAN Yuying, BIAN Yajin, WANG Xueying 94true ［Abstract］　Objective　To analyze the effects of intravenous infusions of human serum albumin(HSA), intravenous immunoglobulin(IVIG), and rabbit anti-human thymocyte immunoglobulin(ATG) on blood transfusion compatibility testing. Methods　The clinical data of 34 patients whose blood samples were received by Department of Transfusion Medicine, the First Medical Center, Chinese People′s Liberation Army General Hospital from January 2022 to December 2023 due to the infusions of albumin products or immunoglobulin(Ig) products resulting in incompatible cross-matching, positive results of unexpected antibody screening, and ABO blood group discrepancies were retrospectively analyzed. ABO/RhD typing test, unexpected antibody screening and identification, cross-matching test, direct antiglobulin test(DAT) and acid elution test were performed by using microcolumn gel method. Results　Among the 10 patients infused with HSA, IgG-type anti-A was detected in the red blood cell(RBC) eluate of 5 patients with type A and 2 patients with type AB, IgG-type anti-B in the RBC eluate of 1 patient with type B, and agglutination phenomenon of B cells in reverse typing occurred in 1 patient with type B, and both IgG-type anti-A and IgG-type anti-B in the RBC eluate of 1 patient with type AB. Among the 18 patients infused with IVIG, IgG-type anti-A was detected in the RBC eluate of 8 patients with type A, IgG-type anti-A in the RBC eluate of 5 patients with type AB, and both IgG-type anti-A and IgG-type anti-B in the RBC eluate of 1 patient with type AB, and agglutination phenomenon of A₁ cells in reverse typing occurred in 3 patients with type A and 1 patient with type AB. Non-specific antibodies were detected in 6 patients infused with ATG. Conclusion　After infusions of HSA, IVIG or ATG, the patients are prone to develop positive DAT results, positive results of unexpected antibody screening, ABO blood group discrepancies, and incompatible cross-matching, which interfere with ABO blood typing and cross-matching.]]> 2025/12/31 13:19:40 TAI Shengfei, CHENG Tingting, XU Binglin, FU Lihui, MA Chunya, YU Yang 93true ［Abstract］　Objective　To explore the associations of interleukin-17A(IL-17A), intercellular adhesion molecule-1(ICAM-1) and mucin 5AC(MUC5AC) levels in bronchoalveolar lavage fluid(BALF) and serum with Mycoplasma pneumoniae pneumonia(MPP) complicating airway mucus plugs(AMP) and clinical prognosis in pediatric patients. Methods　A total of 249 pediatric patients with MPP who were admitted to the Fourth Hospital of Changsha from October 2020 to October 2024 were recruited in a 1∶2 matching design. The pediatric patients were divided into AMP group(83 patients) and non-AMP group(166 patients) according to the occurrence of AMP. BALF and serum specimens were collected before treatment. The levels of IL-17A, ICAM-1 and MUC5AC in BALF and serum were detected by using enzyme-linked immunosorbent assay(ELISA) and were compared between the two groups. The pediatric patients were followed up and their prognosis was observed 28 days after admission for treatment. The factors influencing prognosis of the MPP pediatric patients complicated with AMP were analyzed by using logistic regression. The efficacy of IL-17A, ICAM-1 and MUC5AC levels in BALF and serum in diagnosing the MPP pediatric patients complicated with AMP and the efficacy in predicting the prognosis of the MPP pediatric patients complicated with AMP were analyzed by using receiver operating characteristic(ROC) curve. Results　The levels of IL-17A, ICAM-1 and MUC5AC in BALF and serum in the AMP group were higher than those in the non-AMP group, with statistically significant differences between the two groups(P<0.05). The results of ROC curve analysis showed that the levels of IL-17A, ICAM-1 and MUC5AC in BALF and serum could effectively diagnose the MPP pediatric patients complicated with AMP(P<0.05), and the diagnostic efficacy of the combination of the three indicators was superior to that of a single indicator(P<0.05), and the combined diagnostic efficacy of the three indicators in BALF was comparable to that of the three indicators in serum, and the difference was not statistically significant(Z=1.303, P=0.193). The poor prognosis rate of the MPP pediatric patients complicated with AMP was 36.14%(30/83). The results of multivariate logistic regression analysis showed that higher levels of IL-17A, ICAM-1 and MUC5AC in BALF and serum were independent risk factors for poor prognosis in the MPP pediatric patients complicated with AMP(P<0.05). The results of ROC curve analysis showed that the levels of IL-17A, ICAM-1 and MUC5AC in BALF and serum could effectively predict the poor prognosis of the MPP pediatric patients complicated with AMP(P<0.05), and the diagnostic efficacy of the combination of the three indicators was superior to that of a single indicator(P<0.05). The combined diagnostic efficacy of the three indicators in BALF was comparable to that of the three indicators in serum, and the difference was not statistically significant(Z=1.426, P=0.141). Conclusion　Elevated levels of IL-17A, ICAM-1 and MUC5AC in BALF and serum are associated with the complicating AMP and poor clinical prognosis in MPP pediatric patients. The combined detection of the three indicators is helpful for clinicians to identify the high-risk pediatric patients and perform active interventions and treatments, improving the prognosis of the pediatric patients.]]> 2025/12/31 13:19:40 1, ZOU Deng¹, LEI Chaolan¹, PENG Xinxin², LEI Yuan¹]]> LI Fang¹, ZOU Deng¹, LEI Chaolan¹, PENG Xinxin², LEI Yuan¹ 92true ［Abstract］　Objective　To analyze the application value of VITEK^® 2 AST-YS08 card in drug sensitivity test of non-Candida albicans(NAC). Methods　A total of 159 strains of NAC isolated from the normal germfree sites of the inpatients in Xiamen Hospital of Traditional Chinese Medicine and the First Affiliated Hospital of Xiamen University from January 2017 to December 2021 were selected, including 93 strains of C. parapsilosis complex(80 strains of C. parapsilosis, 8 strains of C.orthopsilosis, 4 strains of C. metapsilosis and 1 strain of Lodderomyces elongisporus), 28 strains of C. tropicalis, 18 strains of C. glabrata(including 1 strain of C. nivariensis), 18 strains of Meyerozyma guilliermondii(including 1 strain of Kodamaea ohmeri) and 2 strains of Pichia kudriavzevii. The minimal inhibitory concentration(MIC) of NAC was tested in parallel using Sensititre YeastOne method(SYO method) and VITEK^® 2 AST-YS08 card, respectively, and the results were interpreted according to the CLSI-M27S44 standard issued by the Clinical and Laboratory Standards Institute(CLSI). Taking the results of SYO method as the reference, the essential agreement(EA), category agreement(CA), very major error(VME), major error(ME) and minor error(mE) of the results were analyzed between VITEK^® 2 AST-YS08 card and SYO method. Results　Taking the results of SYO method as the reference, the EAs of the results in testing the sensitivity of NAC strains to micafungin, fluconazole, voriconazole, 5-flucytosine and caspofungin between VITEK^® 2 AST-YS08 card and SYO method were 91.82% to 100.00%, and the CAs of the results were 86.79% to 95.12%. Among them, the EA of the results in 5-flucytosine sensitivity test was the highest(100.00%), followed by that in voriconazole sensitivity test(99.29%). The VME of the results in caspofungin sensitivity test by using VITEK^® 2 AST-YS08 card was associated with the MIC values of the strains of C. orthopsilosis closing to the antimicrobial breakpoints for interpretation. Conclusion　VITEK^® 2 AST-YS08 card is easy to operate and convenient for automation. Taking the results of SYO method as the reference, the CAs of the results in testing the sensitivity of NAC strains to 5-flucytosine, micafungin, fluconazole, caspofungin and voriconazole between VITEK^® 2 AST-YS08 card and SYO method are higher, and VITEK^® 2 AST-YS08 card has better clinical application value.]]> 2025/12/31 0:00:00 1, XU Heping², ZHENG Yanqing², MA Xiaobo²]]> LIU Weimin¹, XU Heping², ZHENG Yanqing², MA Xiaobo² 91true ［Abstract］　Objective　To analyze the plaque characteristics between small and large infarction lesions in the middle cerebral artery territory using 3.0T high-resolution vessel wall magnetic resonance imaging(HR-VW-MRI) technology. Methods　The clinical data of 63 patients with acute ischemic stroke in the middle cerebral artery territory who were admitted to Beijing Geriatric Hospital from December 2021 to December 2024 were retrospectively analyzed. According to the manifestations of diffusion-weighted imaging and apparent diffusion coefficient, the patients were divided into small subcortical infarction(SSI) group(42 patients) and large subcortical infarction(LSI) group(21 patients). All the patients underwent HR-VW-MRI examination. The number of quadrants with plaque formation, distribution of plaque locations on blood vessel walls, intraplaque hemorrhage, plaque burden(PB), vascular remodeling patterns at the plaque site, and the degree of vascular stenosis at the plaque site were observed in both groups. Results　Among the 42 patients in the SSI group, 34 patients were detected with plaques, totalling 34 plaques. All 21 patients in the LSI group were detected with plaques, totalling 21 plaques. There was statistically significant difference in the number of quadrants with plaque formatin between the two groups(P<0.05), with a higher proportion of the plaques involving only one quadrant in the SSI group. The proportion of intraplaque hemorrhage in the LSI group was higher than that in the SSI group, and the difference was statistically significant(P<0.05). There was no statistically significant difference in PB between the two groups(P>0.05). There was no statistically significant difference in the distribution of plaque locations on blood vessel walls between the two groups(P>0.05), and the plaques in both groups were most commonly found on the ventral and superior abdominal walls, followed by those found on the dorsal abdominal walls, and the plaques on the inferior abdominal walls were the least common. There were no statistically significant differences in vascular remodeling patterns at the plaque site between the two groups(P>0.05), and more than half of the plaques exhibited positive remodeling. There was statistically significant difference in the degree of vascular stenosis at the plaque site between the two groups(P<0.05), with a higher proportion of mild vascular stenosis at the plaque site in the SSI group and a higher proportion of moderate to severe vascular stenosis at the plaque site in the LSI group. Conclusion　There are significant differences in plaque characteristics between SSI and LSI. Compared with those in the LSI area, the plaques in the SSI area are in a more limited involvement range, and have a lesser degree of vascular stenosis at the plaque site, and a lower proportion of intraplaque hemorrhage.]]> 2025/12/31 13:19:40 1, ZHANG Ping¹, ZHANG Yanli², FU Yan², YANG Dandan¹, LIU Jiabao³]]> ZHANG Fengling¹, ZHANG Ping¹, ZHANG Yanli², FU Yan², YANG Dandan¹, LIU Jiabao³ 90true ［Abstract］　Objective　To observe the clinical efficacy of serplulimab in non-small cell lung cancer(NSCLC) and its influence on T cell function. Methods　A total of 162 patients with NSCLC who were admitted to Taizhou People′s Hospital from December 2022 to April 2024 were recruited and divided into serplulimab treatment group(58 patients) and conventional chemotherapy group(104 patients) according to the patients′ treatment intentions. The conventional chemotherapy group received a platinum-based concurrent chemotherapy regimen, while the serplulimab treatment group received the same chemotherapy regimen as the conventional chemotherapy group plus an additional serplulimab treatment. All the patients were treated continuously for 2 cycles(3 weeks as 1 treatment cycle). The levels of T lymphocyte subsets and tumor markers were compared between the patients in the two groups before and after treatment. The therapeutic effects were evaluated according to Response Evaluation Criteria in Solid Tumors version 1.1(RECIST 1.1). After a one-year follow-up, the progression-free survival and the occurrence of adverse reactions were compared between the two groups. Results　After treatment, the levels of serum carcinoembryonic antigen(CEA), carbohydrate antigen 125(CA125), carbohydrate antigen 199(CA199) and cytokeratin fragment antigen 21-1(CYFRA21-1) in both groups were significantly lower than those before treatment(P<0.05), but there were no statistically significant differences in the levels of these indicators between the two groups after treatment(P>0.05). Compared with those in the conventional chemotherapy group, the levels of CD4⁺ and CD4⁺/CD8⁺ ratio in the serplulimab treatment group were higher after treatment(P<0.05), and the differences were statistically significant(P<0.05). The objective response rate and disease control rate of the patients in the serplulimab treatment group were significantly higher than those in the conventional chemotherapy group(58.62% vs 37.50%, χ²=6.709, P=0.010; 82.76% vs 60.58%, χ²=8.493, P=0.004). The median progression-free survival in the serplulimab treatment group was 12 months and that in the conventional chemotherapy group was 9 months. The prognosis of progression-free survival in the serplulimab treatment group was significantly better than that in the conventional chemotherapy group(log-rank test: χ²=10.931, P=0.001). There was no statistically significant difference in the total incidence of adverse reactions between the two groups(P>0.05). Conclusion　Serplulimab has good clinical therapeutic effects on NSCLC. It can effectively improve T lymphocyte immune function and levels of serum tumor markers in the NSCLC patients, and has clinical promotion value.]]> 2025/12/31 13:19:40 YANG Xiuyuan, YU Lei 89true ［Abstract］　Objective　To observe the application effect of individualized treatments for ischemic stroke patients guided by the results of clopidogrel-related gene polymorphism and platelet aggregation tests. Methods　A total of 120 patients with ischemic stroke who were admitted to Department of Neurology, Wuzhou Gongren Hospital from January 2022 to January 2024 were recruited and divided into research group and control group by random number table method, with 60 patients in each group. The control group was given conventional antiplatelet therapy［oral administration of clopidogrel(75 mg q.d.) and aspirin(100 mg q.d.)］. The research group was given conventional oral administration of aspirin(100 mg q.d.), and individualized treatment regimens were formulated according to the results of clopidogrel-related genes［including CYP2C19*2(rs4244285)、CYP2C19*3(rs4986893)、CYP2C19*17(rs12248560), ABCB1 C3435T(rs1045642) and CES1 G143E(rs71647871)］ test and platelet aggregation test. The patients with CYP2C19 gene phenotypes of ultrarapid metabolizer(UM) and extensive metabolizer(EM) and ABCB1 C3435T carrying the G allele were treated with clopidogrel. The patients with CYP2C19 gene phenotypes of intermediate metabolizer(IM) and poor metabolizer(PM) and ABCB1 C3435T carrying the A allele were treated with ticagrelor. The patients with CYP2C19 gene phenotype of EM and ABCB1 C3435T of AA type, as well as those with CYP2C19 gene phenotype of IM and ABCB1 C3435T of GG type, were initially treated with clopidogrel. After 5 days of the medication, if the platelet adenosine diphosphate(ADP) receptor aggregation rate was less than 30%, clopidogrel treatment should be continued, and if the platelet ADP receptor aggregation rate was greater than or equal to 30%, clopidogrel treatment should be switched to ticagrelor treatment. If the patients′ CES1 G143E was CT type or TT type, ticagrelor was used for the treatment. The patients were followed up for 1 year to compare the occurrence of major adverse cardiovascular and cerebrovascular events(MACCE) between the two groups, and to analyze the impact of different genotypes on the prognosis of the patients receiving individualized treatment of clopidogrel. Results　According to the results of clopidogrel-related gene polymorphism and platelet aggregation tests, a total of 28 patients in the research group had their medications adjusted. During the 1-year follow-up, no patients were lost to follow-up in either group. There were 21 cases of MACCE in the control group and 7 cases of MACCE in the research group. The research group had better results in terms of MACCE-free situation［HR(95%CI)=3.37(1.55-7.31), P=0.002］. In the patients of the research group, there was no statistically significant difference in the occurrence of MACCE between the patients with CYP2C19 gene phenotypes of UM type/EM type and those with CYP2C19 gene phenotypes of IM type/PM type［HR(95%CI)=1.92(0.42-8.85), P=0.400］, and there was no statistically significant difference in the occurrence of MACCE between the patients with ABCB1 C3435T gene of GG type and those with ABCB1 C3435T gene of GA type/AA type［HR(95%CI)=0.34(0.07-1.57), P=0.165］. Conclusion　Individualized treatments guided by the results of clopidogrel-related gene polymorphism and platelet aggregation tests can reduce the risk of MACCE in ischemic stroke patients.]]> 2025/12/31 13:19:40 1, HUANG Jinshi¹, LI Tao¹, CHEN Yingdao², CHEN Xiaoling², WU Lifang¹]]> LI Zhaoquan¹, HUANG Jinshi¹, LI Tao¹, CHEN Yingdao², CHEN Xiaoling², WU Lifang¹ 88true ［Abstract］　Objective　To compare therapeutic effects of programmed cell death protein-1(PD-1) inhibitors combined with neoadjuvant chemotherapy(NAT) versus simple NAT in treatment of locally advanced head and neck squamous cell carcinoma(LA HNSCC). Methods　A retrospective analysis was conducted on the clinical data of 140 patients with LA HNSCC who were admitted to Sun Yat-sen University Cancer Center from February 2012 to February 2023. According to different therapeutic regimens received by the patients, they were divided into immunochemotherapy group(118 patients) and simple chemotherapy group(22 patients). The baseline characteristics were balanced by using propensity score overlap weighting method. The simple chemotherapy group received a TP-based NAT regimen. The immunochemotherapy group received a regimen of intravenous infusion of a PD-1 inhibitor(200 mg) on the first day of each treatment cycle in addition to the TP-based NAT. After the NAT, the patients underwent radical surgical treatment. The objective response rate(ORR), pathological downstaging rate, pathological complete response(pCR) rate, overall survival(OS), and progression-free survival(PFS) were compared between the two groups. Cox regression model was used to analyze the factors influencing the survival prognosis of the LA HNSCC patients. Results　The ORR, pathological downstaging rate and pCR rate in the immunochemotherapy group were higher than those in the simple chemotherapy group, with statistically significant differences between the two groups(P<0.05). By August 2025, the median follow-up time for the immunochemotherapy group was 33.81 months, with 22 cases of disease progression and 15 deaths, and the median follow-up time for the simple chemotherapy group was 24.32 months, with 12 cases of disease progression and 12 deaths. The OS and PFS in the immunochemotherapy group were significantly better than those in the simple chemotherapy group［HR(95%CI)=0.233(0.106-0.512), P<0.001; HR(95%CI)=0.323(0.155-0.672), P=0.002］. After the matching of propensity score overlap weighting method, the OS and PFS in the immunochemotherapy group were still significantly better than those in the simple chemotherapy group［HR(95%CI)=0.236(0.073-0.759), P=0.016; HR(95%CI)=0.337(0.122-0.936), P=0.037］. The results of multivariate Cox regression analysis showed that age ≥60 years was an independent risk factor for shortening OS and PFS in the LA HNSCC patients(P<0.05), and the combined immunotherapy was an independent protective factor for prolonging OS and PFS in the LA HNSCC patients(P<0.05). Conclusion　Compared with the simple NAT, the combination of PD-1 inhibitors and NAT can significantly improve the survival prognosis of LA HNSCC patients and the combination therapy is worthy of clinical recommendation.]]> 2025/11/29 21:17:25 JIAO Zan, YU Yongchao, JIANG Mingjie, WU Tong, PENG Jin, NING Kang, YANG Ankui 87true ［Abstract］　Objective　To explore the effects of high expression of insulin-like growth factor 2 mRNA-binding protein 1(IGF2BP1) on paclitaxel-resistant ovarian cancer cells and its mechanisms. Methods　Kaplan-Meier Plotter database and ROC Plotter database were used to analyze the correlations of IGF2BP1 expression with the ovarian cancer patients′ prognosis and paclitaxel resistance, respectively. The ovarian cancer cell line SKOV3 sensitive to paclitaxel and the ovarian cancer cell line SKOV3-R resistant to paclitaxel were selected for experiment. IGF2BP1-overexpressing lentivirus and empty vector were transfected into SKOV3-R cells, which were named S-R-IGF2BP1-OE cells and S-R-EV cells, respectively. Cell proliferation activity was detected by using CCK-8 assay. The expression of IGF2BP1 mRNA was detected by using reverse transcription-quantitative real-time polymerase chain reaction(RT-qPCR). Cell cycle distribution was detected by using flow cytometry. The expression levels of cell cycle-related factors were detected by using Western blot assay. Results　High expression of IGF2BP1 was significantly correlated with worse overall survival(OS) and progression-free survival(PFS) in the ovarian cancer patients(P<0.05). The expression level of IGF2BP1 mRNA in the chemotherapy-resistant group was significantly higher than that in the chemotherapy-sensitive group(P<0.05). The expression level of IGF2BP1 mRNA in SKOV3-R cells was significantly higher than that in SKOV3 cells(P<0.05). Under the intervention of paclitaxel, the proliferation activity of S-R-IGF2BP1-OE cells was higher than that of S-R-EV cells(P<0.05). The half maximal inhibitory concentration(IC₅₀) of paclitaxel for S-R-IGF2BP1-OE cells and the IC₅₀ of paclitaxel for S-R-EV cells were 187.02 nmol/L and 98.76 nmol/L, respectively. The proportions of G₀/G₁ phase and G₂/M phase in S-R-IGF2BP1-OE cells were significantly lower than those in S-R-EV cells(P<0.05). The expression level of cyclin A2 in S-R-IGF2BP1-OE cells was higher than that in S-R-EV cells, and the expression levels of p27 kinase inhibitor protein 1(p27 KIP1), p27 CDK-interacting protein 1(p21 Cip1), glycogen synthase kinase-3β(GSK-3β), and cell cyclin-dependent kinase 7(CDK7) in S-R-IGF2BP1-OE cells were lower than those in S-R-EV cells, and the differences were statistically significant(P<0.05). Conclusion　High expression of IGF2BP1 is associated with poor prognosis in ovarian cancer patients, which may mediate paclitaxel resistance by regulating the cell cycle of ovarian cancer cells.]]> 2025/11/29 21:17:25 1, CHEN Xiaoying², LIU Xia², YIN Fuqiang¹]]> YU Yue¹, CHEN Xiaoying², LIU Xia², YIN Fuqiang¹ 86true ［Abstract］　Objective　To analyze the pregnancy outcomes and influencing factors after cardiac surgery under cardiopulmonary bypass in pregnancy. Methods　The clinical data of 56 patients who underwent cardiac surgery under cardiopulmonary bypass(CPB) in pregnancy in Department of Cardiac Surgery, Guangdong Provincial People′s Hospital from January 2010 to December 2023 were retrospectively analyzed. According to whether the fetuses survived 1 week after surgery, the patients were divided into fetal survival group(45 patients) and fetal death group(11 patients). The clinical data were compared between the two groups. Multivariate logistic regression was used to analyze the influencing factors of fetal death. Results　There were no maternal deaths among the 56 patients. Among the 45 patients of the fetal survival group, 1 case belonged to a twin pregnancy. There were 11 cases in the fetal death group, all of whom were singleton pregnancies. The fetal mortality rate was 19.30%(11/57). The proportion of patients receiving metoprolol treatment during pregnancy in the fetal survival group was higher than that in the fetal death group, and the duration of operation and duration of CPB in the fetal survival group were shorter than those in the fetal death group, and the intraoperative blood loss in the fetal survival group was less than that in the fetal death group, and the perioperative blood transfusion rate in the fetal survival group was lower than that in the fetal death group, and the proportion of patients receiving atosiban treatment during the perioperative period in the fetal survival group was higher than that in the fetal death group, with statistically significant differences between the two groups(P<0.05). The results of multivariate logistic regression analysis showed that perioperative blood transfusion was an independent risk factor for fetal death, while receiving metoprolol treatment during pregnancy and receiving atosiban treatment during the perioperative period were independent protective factors for inhibiting fetal death(P<0.05). Conclusion　Cardiac surgery under CPB during pregnancy poses a greater threat to the fetuses than to their mothers. Perioperative blood transfusion is an independent risk factor for fetal death. Receiving metoprolol treatment during pregnancy and receiving atosiban treatment during the perioperative period are independent protective factors for inhibiting fetal death.]]> 2025/11/29 0:00:00 1,2, WANG Zhenzhong^1,2, ZHAO Junfei^1,2, ZHANG Chaolong^1,2, KE Yingjie^1,2,3, WANG Qiuji^1,2, ZHONG Lishan^1,2, PANG Shanwen^1,2, QIU Junqiang^1,2, LIU Yanli⁴, ZHANG Chen⁵, HUANG Huanlei^1,2]]> LI Yuxin^1,2, WANG Zhenzhong^1,2, ZHAO Junfei^1,2, ZHANG Chaolong^1,2, KE Yingjie^1,2,3, WANG Qiuji^1,2, ZHONG Lishan^1,2, PANG Shanwen^1,2, QIU Junqiang^1,2, LIU Yanli⁴, ZHANG Chen⁵, HUANG Huanlei^1,2 85true ［Abstract］　Objective　To investigate the association of Epstein-Barr virus(EBV) infection with the severity of connective tissue disease-associated interstitial lung disease(CTD-ILD) and immune dysregulation. Methods　A retrospective analysis was conducted on the clinical data of 63 CTD-ILD patients(CTD-ILD group) who were admitted to Xiangyang Central Hospital Affiliated to Hubei University of Arts and Science from September 2023 to January 2025. Forty-five patients with connective tissue disease(CTD)(CTD group) and 45 healthy individuals undergoing physical examinations(control group) were selected according to the matching criteria including gender, age, and body mass index(BMI). The EBV-DNA load was detected by using real-time fluorescence quantitative polymerase chain reaction(FQ-PCR). According to the detection results, the CTD-ILD patients were further divided into EBV-DNA positive group(22 patients) and EBV-DNA negative group(41 patients). The levels of T lymphocyte subsets were detected by using flow cytometry, and the levels of serum interferon -γ(IFN-γ) and interleukin-4(IL-4) were detected by using enzyme-linked immunosorbent assay(ELISA). The effect of the use of immunosuppressants on the risk of EBV infection in the CTD-ILD patients was analyzed. Results　The positive rates of EBV-DNA in the CTD-ILD group, the CTD group and the control group were 34.92%(22/63), 17.78%(8/45) and 6.67%(3/45), respectively, with statistically significant differences among the three groups(χ²=12.929 P=0.002). The positive rate of EBV-DNA in the CTD-ILD group was significantly higher than that in the CTD group and the control group(P<0.05), while there was no statistically significant difference in the positive rate of EBV-DNA between the CTD group and the control group(P>0.05). The use of immunosuppressants did not significantly affect the risk of EBV infection in the CTD-ILD patients(P>0.05). In the CTD-ILD patients, the levels of forced vital capacity percent predicted(FVC% pred), percent predicted diffusing capacity of the lung for carbon monoxide(DLCO% pred), IFN-γ, CD4⁺ and CD4⁺/CD8⁺ ratio in the EBV-DNA positive group were lower than those in the EBV-DNA negative group, while the high-resolution computed tomography(HRCT) scores, IL-4 and CD8⁺ levels in the EBV-DNA positive group were higher than those in the EBV-DNA negative group, with statistically significant differences between the two groups(P<0.05). The results of Pearson correlation analysis showed that in the CTD-ILD patients, IL-4 was negatively correlated with FVC% pred and DLCO% pred(P<0.05), and positively correlated with HRCT scores(P<0.05), and the IFN-γ, CD4⁺ and CD4⁺/CD8⁺ ratio were positively correlated with FVC% pred and DLCO% pred(P<0.05), and negatively correlated with HRCT scores(P<0.05). Conclusion　EBV infection is closely related to the severity of pulmonary interstitial fibrosis in CTD-ILD patients, and the mechanism may be related to immune dysregulation and persistent inflammation.]]> 2025/11/29 21:17:25 1, HUANG Xin¹, ZENG Xiansheng²]]> MA Qiao¹, HUANG Xin¹, ZENG Xiansheng² 84true ［Abstract］　Objective　To explore the therapeutic effect of anemoside B4(AB4) on androgenetic alopecia(AGA) in mice and its mechanism of action. Methods　Forty-eight male C57BL/6 mice were selected and randomly divided into control group, model group, low-dose AB4 group, medium-dose AB4 group, high-dose AB4 group and 5% minoxidil tincture group, with 8 mice in each group. The mice in the control group were fed normally without special interventions. The model group and each intervention group were topically applied with testosterone solution［10 mg/(kg·d)］ on the skin on the neck and back of the mice to establish the AGA model. After construction of the AGA model, the model group was topically applied with 20% glycerol solution on the skin on the neck and back of the mice, twice daily, 0.2 mL per time, and the low-dose AB4 group, the medium-dose AB4 group and the high-dose AB4 group were topically applied with 5 mg/mL, 10 mg/mL and 20 mg/mL AB4 solution on the skin on the neck and back of the mice, respectively, twice daily, 0.2 mL per time, and the 5% minoxidil tincture group was topically applied with 5% minoxidil tincture on the skin on the neck and back of the mice, twice daily, 0.1 mL per time. The interventions lasted for 23 days, during which testosterone solution［5 mg/(kg·d)］ was topically applied on the skin on the neck and back of the mice to maintain the AGA model. The hair growth of the mice was observed and recorded by taking photos every week, and skin hair follicles were observed by using hematoxylin and eosin(HE) staining after the last intervention. The serum testosterone, estradiol(E₂) and type Ⅱ 5-α reductase(5AR2) levels were detected by using enzyme-linked immunosorbent assay(ELISA) and the testosterone/E₂ ratio was calculated. The levels of transforming growth factor-β1(TGF-β1), tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β) and interleukin-6(IL-6) in the skin tissues of the neck and back of the mice were detected. Results　After 23 days of interventions, compared with the model group, the number of skin hair follicles in the AB4 intervention groups was significantly increased(P<0.05), with larger hair follicle volume. Moreover, the number of hair follicles in the medium-dose AB4 group and the high-dose AB4 group was larger than that in the 5% minoxidil tincture group(P<0.05). The levels of serum testosterone, 5AR2 and testosterone/E₂ ratio in the AB4 intervention groups were significantly lower than those in the model group(P<0.05), while the levels of E₂ in the AB4 intervention groups were significantly higher than those in the model group(P<0.05). The level of serum E₂ in the high-dose AB4 group was higher than that in the 5% minoxidil tincture group, and the testosterone/E₂ ratio in the high-dose AB4 group was lower than that in the 5% minoxidil tincture group, with statistically significant differences(P<0.05). The levels of TGF-β1 and IL-6 in skin tissues of the AB4 intervention groups were significantly lower than those in the model group and the 5% minoxidil tincture group(P<0.05). Conclusion　AB4 may exert therapeutic effect on AGA by regulating serum hormone balance and inhibiting skin inflammatory response.]]> 2025/11/29 21:17:25 1,2, WANG Qinqin³, GAO Xiang¹, XIE Xiaoyun¹, CHEN Xiate¹, YUAN Renyikun¹, GAO Hongwei¹, YANG Shilin¹]]> LIU Xiaoyan^1,2, WANG Qinqin³, GAO Xiang¹, XIE Xiaoyun¹, CHEN Xiate¹, YUAN Renyikun¹, GAO Hongwei¹, YANG Shilin¹ 83true ［Abstract］　Objective　To observe the therapeutic effect of modified Xiaotong Anshen prescription in adjuvant treatment of gouty nephropathy(GN) and to explore the related mechanisms. Methods　A total of 106 patients with GN who were admitted to Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine from April 2021 to March 2024 were recruited. The stages of chronic kidney disease of these patients were stages 3 to 4. The GN patients were divided into observation group and control group by using random number table method, with 53 patients in each group. The control group was treated with conventional Western medicine, while the observation group was treated with conventional Western medicine plus modified Xiaotong Anshen prescription. The treatment course for both groups was 3 weeks. The clinical efficacy, as well as the scores of traditional Chinese medicine(TCM) symptoms, renal function indicators［blood urea nitrogen(BUN), serum creatinine(Scr), 24-hour urine total protein(24 h UTP), β₂-microglobulin(β₂-MG)］, serum uric acid, erythrocyte sedimentation rate(ESR), and the levels of proteins related to the phosphoinositide 3-kinase(PI3K)/threonine kinase(AKT)/nuclear factor-κB(NF-κB) signaling pathway were compared between the two groups before and after treatment. The occurrence of adverse reactions during the patients′ treatment period was recorded. Results　The total clinical effective rate of the observation group was significantly higher than that of the control group(94.34% vs 77.36%, χ²=6.290, P=0.012). After treatment, the TCM syndrome scores of joint skin redness and swelling, joint burning, joint pain, facial or lower limb edema, and heavy limbs in both groups were lower than those before treatment, and the TCM syndrome scores of the observation group were lower than those of the control group after treatment, with statistically significant differences(P<0.05). After treatment, the levels of BUN, Scr, 24 h UTP, β₂-MG, serum uric acid and ESR in both groups were lower than those before treatment, and the levels in the observation group were lower than those in the control group after treatment, with statistically significant differences(P<0.05). After treatment, the levels of phosphorylated PI3K(p-PI3K), phosphorylated AKT(p-AKT), and phosphorylated NF-κB(p-NF-κB) proteins in both groups were lower than those before treatment, and the levels in the observation group were lower than those in the control group after treatment, with statistically significant differences(P<0.05). During the treatment period, 1 case of diarrhea occurred in the observation group, and 2 cases of nausea and 2 cases of constipation occurred in the control group. There was no statistically significant difference in the total incidence of adverse reactions between the two groups(P>0.05). Conclusion　The modified Xiaotong Anshen prescription combined with Western medicine has definite effect and good safety in treatment of GN. It may play a therapeutic role through the PI3K/AKT/NF-κB signaling pathway.]]> 2025/11/29 21:17:25 LI Li, MA Jinrong, ZHAO Li, PAN Hongmei, ZHANG Linyan, YAO Shuoshuo, LI Yuelin 82true ［Abstract］　Objective　To analyze the expression characteristics of CD38 and CD138 in endometria of patients with failed in vitro fertilization-embryo transfer(IVF-ET) and their correlations with chronic endometritis(CE). Methods　A total of 114 patients who underwent IVF-ET but failed to conceive in Changzhou Maternal and Child Health Care Hospital from April 2023 to September 2024 were recruited. All the patients underwent hysteroscopy and their endometrial tissues were obtained. The endometrial tissues of the patients were stained by using hematoxylin-eosin(HE) and the pathological typing was performed. The expressions of CD38 and CD138 were detected by using immunohistochemical staining, and according to the detection results the patients were divided into CD38⁺/CD138⁺ group(34 cases), CD38⁺/CD138^- group(37 cases), CD38^-/CD138⁺ group(23 cases) and CD38^-/CD138^- group(20 cases). The associations of the expressions of CD38 and CD138 with the occurrence of CE as well as the pregnancy outcomes of the patients who received assisted reproduction after doxycycline treatment were analyzed. Results　In the 114 patients, the incidence of CE was 38.60%(44/114). There was a statistically significant difference in the incidence of CE among the four groups(P<0.05), with the highest incidence of CE in the CD38⁺/CD138⁺ group(70.59%). The difference in the proportion of normal endometria was statistically significant among the four groups(P<0.05), with the highest normal rate(55.00%) in the CD38^-/CD138^- group. Among the 94 patients treated with doxycycline, 48 patients(51.06%) became pregnant after assisted reproduction. The pregnancy rates of the CD38⁺/CD138⁺ group, the CD38⁺/CD138^- group and the CD38^-/CD138⁺ group were 35.29%(12/34), 67.57%(25/37) and 47.83%(11/23), respectively. There was a statistically significant difference in the pregnancy rate among the three groups(χ²=7.513, P=0.023), and the pregnancy rate of the CD38⁺/CD138⁺ group was significantly lower than that of the CD38⁺/CD138^- group(χ²=7.395, P=0.007). The results of multivariate logistic regression analysis showed that CD38⁺/CD138⁺ was an independent risk factor for pregnancy failure in patients after they received doxycycline treatment［OR(95%CI)=3.749(1.093-12.862), P=0.036］. Conclusion　The expressions of CD38 and CD138 are related to the occurrence of CE in patients with failed IVF-ET implantation and have impacts on the therapeutic effect of doxycycline. The individualized treatment strategies carried out on the basis of these results may help to improve the pregnancy outcomes of the patients.]]> 2025/11/29 0:00:00 1, XUE Pingping¹, ZHU Li², Baktnur Kenjekul³, ZHUANG Jing¹]]> MIAO Jun¹, XUE Pingping¹, ZHU Li², Baktnur Kenjekul³, ZHUANG Jing¹ 81true ［Abstract］　Objective　To analyze the effects of chemotherapy on survival prognosis of patients with distant metastatic nasopharyngeal carcinoma. Methods　A retrospective analysis was conducted on the clinical data and follow-up data of 187 patients with distant metastatic nasopharyngeal carcinoma who were admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region from June 2010 to June 2019. Among them, 100 patients were willing to receive chemotherapy(chemotherapy group), and 87 patients abandoned chemotherapy(non-chemotherapy group). The effects of factors such as metastatic sites, chemotherapy regimens and chemotherapy cycles on the patients′ survival prognosis were analyzed by using survival curve and Cox regression. Results　By January 2020, 82 patients in the non-chemotherapy group and 70 patients in the chemotherapy group died. The survival prognosis of the patients in the chemotherapy group was significantly better than that of the patients in the non-chemotherapy group(log-rank test: χ²=9.565, P=0.002). For the patients receiving chemotherapy, the results of multivariate Cox regression analysis showed that, with lung metastasis as a reference, liver metastasis［RR(95%CI)=2.823(1.284-6.210)］ and multiple metastases［RR(95%CI)=3.920(2.118-7.255)］ were independent risk factors for poor survival prognosis in them(P<0.05); with chemotherapy cycles ≤4 cycles as a reference, chemotherapy cycles >4 cycles［RR(95%CI)=0.276(0.142-0.536)］ were independent protective factors for promoting better survival prognosis in them(P<0.05). Conclusion　Chemotherapy can significantly improve the survival prognosis of patients with distant metastatic nasopharyngeal carcinoma. Metastatic site and chemotherapy cycle are important factors affecting the patients′ survival prognosis.]]> 2025/11/29 21:17:25 QIN Yangda, LI Min, WANG Yongli, HUANG Bo, XIE Sifang, WENG Jingjin 80true ［Abstract］　Objective　To observe the application effect of Artificial Intelligence of Things(AIoT) wearable devices for walking with the function of treating stroke on rehabilitation training of ischemic stroke patients. Methods　One hundred patients with ischemic stroke who were admitted to Longgang Central Hospital of Shenzhen from January 2023 to December 2024 were selected. The patients were divided into control group and observation group according to random number table method, with 50 patients in each group. The control group received routine rehabilitation training, while the observation group received walking training with AIoT-based wearable devices with the function of treating stroke plus daily routine rehabilitation training. The Holden Functional Walking Classification, modified Ashworth Scale for lower limb muscle tension, Brunnstrom Classification for lower limbs, joint range of motion and gait conditions were compared between the patients in the two groups before treatment and 4 weeks after treatment. Results　Before treatment, there were no statistically significant differences in the grades of Holden Functional Walking Classification, grades of modified Ashworth Scale for lower limb muscle tension, grades of Brunnstrom Classification for lower limbs, joint range of motion and gait conditions between the two groups(P>0.05). After treatment, the grades of Holden Functional Walking Classification, grades of modified Ashworth Scale for lower limb muscle tension, grades of Brunnstrom Classification for lower limbs, joint range of motion and gait conditions in the two groups were improved compared with those before treatment, and the improvements in the observation group were better than those in the control group, with statistically significant differences. Conclusion　The application of AIoT-based wearable devices for walking with the function of treating stroke in rehabilitation training for patients with ischemic stroke can significantly improve their walking function, lower limb muscle tension, motor function and joint range of motion.]]> 2025/11/29 0:00:00 WANG Lei, SU Haoqiang, MA Hongdan, ZHU Meiying, OU Jiayuan, LI Jinbao, LYU Zheng 79true ［Abstract］　Objective　To construct and validate a nomogram prediction model for multiple myeloma(MM) complicated with nervous system damage. Methods　A retrospective analysis was conducted on the clinical data of 202 patients with MM who were admitted to the Affiliated Hospital of Southwest Medical University from October 2019 to October 2023. The patients were divided into the occurrence group(86 patients) and the non-occurrence group(116 patients) according to the occurrence of nervous system damage during the follow-up period. Multivariate logistic regression was used to analyze the influencing factors of MM complicated with nervous system damage. A nomogram prediction model for MM complicated with nervous system damage was constructed. The predictive efficacy of the model for MM complicated with nervous system damage was evaluated using receiver operating characteristic(ROC) curve, calibration curve, Hosmer-Lemeshow goodness-of-fit test and decision curve analysis(DCA). Results　The incidence of MM complicated with nervous system damage was 42.57%. The results of multivariate logistic regression analysis showed that older age, higher levels of lactate dehydrogenase(LDH) and immunoglobulin(Ig) G, and International Staging System(ISS) stage Ⅲ were independent risk factors for MM complicated with nervous system damage(P<0.05), and longer activated partial thromboplastin time(APTT), longer prothrombin time(PT), and higher hemoglobin level were independent protective factors for MM complicated with nervous system damage(P<0.05). Based on the above 7 influencing factors, a nomogram prediction model for MM complicated with nervous system damage was constructed. The results of ROC curve analysis showed that the area under the curve of the model for predicting MM complicated with nervous system damage was 0.917(95%CI: 0.861-0.956), and the sensitivity and specificity were 95.35% and 87.85%, respectively. The Hosmer-Lemeshow goodness-of-fit test results(χ²=2.944, P=0.872) proved that the model had an adequate goodness-of-fit. The calibration curve showed that the predicted probability of the model was close to the actual probability, indicating a good calibration. DCA showed that net clinical benefits could be achieved when the prediction threshold probability of the model was between 20% and 93%, or between 94% and 100%. Conclusion　The nomogram prediction model which is constructed for MM complicated with nervous system damage based on 7 influencing factors including age, LDH, IgG, APTT, PT, hemoglobin and ISS stage has good predictive value.]]> 2025/11/29 21:17:25 MI Qiuwen, LUO Lixiu, WU Yanling, WEN Xiaoying, ZHAO Yu, LIU Yang, CHEN Xiaomin, HUANG Chunlan 78true ［Abstract］　Objective　To construct a nomogram model for predicting postoperative delirium(POD) in non-hyperlipidemia patients undergoing esophageal cancer resection based on plasma total cholesterol(TC) levels. Methods　A retrospective analysis was conducted on the clinical data of 187 non-hyperlipidemia patients who underwent esophageal cancer resection in the 960th Hospital of the PLA Joint Logistics Support Force from January 2019 to December 2023. According to the occurrence of delirium within 1 week after the operation, the patients were divided into delirium group(24 patients) and non-delirium group(163 patients). Multivariate logistic regression was used to analyze factors influencing the incidence of POD in the patients undergoing esophageal cancer resection, and a nomogram prediction model was constructed. The predictive efficacy of the model was verified by using Hosmer-Lemeshow goodness-of-fit test, Bootstrap resampling method, receiver operating characteristic(ROC) curve and decision curve analysis(DCA). Results　Compared with the non-delirium group, the delirium group was older, and had a lower proportion of patients receiving dexmedetomidine treatment, lower levels of intraoperative minimum mean arterial pressure(MAP) and total cholesterol(TC), and low-density lipoprotein cholesterol(LDL-C), longer duration of anesthesia and postoperative hospital stay, and a higher proportion of patients with coronary heart disease, a higher proportion of patients transferred to intensive care unit(ICU) after surgery and a higher proportion of patients who died within 30 days after surgery, with statistically significant differences(P<0.05). The results of multivariate logistic regression analysis showed that a higher TC level, a higher intraoperative minimum MAP, and use of dexmedetomidine were independent protective factors for inhibiting the incidence of POD in the patients undergoing esophageal cancer resection(P<0.05). A nomogram prediction model was constructed based on the above three influencing factors. The results of Hosmer-Lemeshow goodness-of-fit test showed that the model fitted well(χ²=3.763, P=0.878). The C-index value obtained by the Bootstrap resampling method was 0.897, indicating that the model had a relatively high prediction consistency. The ROC curve showed that area under the curve(AUC)(95%CI) of the model for predicting POD in patients undergoing esophageal cancer resection was 0.919(0.870-0.969), with specificity of 79.10% and sensitivity of 91.70%. The DCA curve showed that the net benefit of the model was relatively high and stable within the risk thresholds ranging from 0.05 to 0.85, indicating that the model had good clinical value. Conclusion　Higher plasma TC level, higher intraoperative minimum MAP and use of dexmedetomidine are independent protective factors for inhibiting the incidence of POD in non-hyperlipidemic patients undergoing esophageal cancer resection. The nomogram prediction model constructed based on the above three factors has good predictive value for POD.]]> 2025/10/31 0:00:00 1, LI Yun², LI Yan¹, MA Guodong¹, GAO Chengjie¹]]> GAO Shengrun¹, LI Yun², LI Yan¹, MA Guodong¹, GAO Chengjie¹ 77true ［Abstract］　Objective　To construct a nomogram model based on Injury Severity Scores(ISS) and coagulation-related indicators to predict intra-abdominal rebleeding after thoracotomy in emergency patients with thoracoabdominal injuries, and to analyze its clinical application value. Methods　A retrospective analysis was conducted on the clinical data of 205 emergency patients with thoracoabdominal injuries who were admitted to Honghui Hospital Affiliated to Xi′an Jiaotong University from September 2021 to June 2023. All the patients were treated with thoracotomy. According to the occurrence of intra-abdominal rebleeding after surgery, the patients were divided into rebleeding group(n=73) and non-rebleeding group(n=132). The data such as preoperative platelet-to-lymphocyte ratio(PLR), platelet alpha granule membrane protein-140(GMP-140), ISS, prothrombin time(PT), activated partial thromboplastin time(APTT) and fibrinogen(FIB) were compared between the two groups. Multivariate logistic regression was used to analyze the factors influencing intra-abdominal rebleeding after thoracotomy in the emergency patients with thoracoabdominal injuries. Based on the results of multivariate logistic regression analysis, a nomogram model was constructed to predict intra-abdominal rebleeding after thoracotomy in the emergency patients with thoracoabdominal injuries. The predictive efficacy and application value of the nomogram model were analyzed by using receiver operating characteristic(ROC) curve, calibration curve and decision curve. Results　The levels of ISS, PT, APTT, PLR and GMP-140 in the rebleeding group were higher than those in the non-rebleeding group, and the level of FIB in the rebleeding group was lower than that in the non-rebleeding group, with statistically significant differences between the two groups(P<0.05). The results of multivariate logistic regression analysis showed that higher levels of ISS, PT, APTT, FIB, PLR and GMP-140 were independent risk factors for intra-abdominal rebleeding after thoracotomy in the emergency patients with thoracoabdominal injuries(P<0.05). A nomogram model for predicting intra-abdominal rebleeding after thoracotomy in the emergency patients with thoracoabdominal injuries was constructed based on ISS, PT, APTT, FIB, PLR and GMP-140 indicators. The results of ROC curve analysis showed that the nomogram model had good predictive efficacy［AUC(95%CI)=0.913(0.874-0.952)］, with a sensitivity of 86.30% and a specificity of 89.39%. The results of calibration curve analysis showed that the actual curve was basically in line with the calibration curve, suggesting that the nomogram model had good predictive efficacy. The results of decision curve analysis showed that within the threshold probability ranging from 0.10 to 0.81, the clinical utility of the nomogram was good. Conclusion　The nomogram model constructed in this study has good clinical application value for predicting intra-abdominal rebleeding after thoracotomy in emergency patients with thoracoabdominal injuries. It is helpful for clinical screening of high-risk patients, providing guidance for early intervention, and reducing the rate of secondary surgery and mortality.]]> 2025/10/31 10:05:06 1, YAO Tingting², ZHENG Yan¹, ZHOU Ting³, YANG Ya′nan⁴]]> WU Dinghua¹, YAO Tingting², ZHENG Yan¹, ZHOU Ting³, YANG Ya′nan⁴ 76true ［Abstract］　Objective　To observe the efficacy of integrated neuromuscular training(INT) in treatment of patients with spastic hemiplegia of lower limbs after stroke. Methods　One hundred patients with spastic hemiplegia of lower limbs after stroke who were admitted to Chinese People′s Liberation Army Marine Corps Hospital from January 2021 to December 2024 were selected. The patients were divided into control group and observation group by using random number table method, with 50 patients in each group. The control group received conventional rehabilitation training, while the observation group received INT. The training period was 12 weeks in both groups. The surface electromyography(sEMG) indicators of lower limbs, neurological function, lower limb motor function, psychological state and quality of life were observed, and the levels of serum gamma-aminobutyric acid(GABA), neuron-specific enolase(NSE) and glutamate(Glu) were detected before and after treatment. The clinical effects were compared between the two groups. Results　After treatment, the root mean square(RMS) of electrical activity signals of muscles in electromyography, the National Institutes of Health Stroke Scale(NIHSS) scores, the Clinical Spasticity Index(CSI) scores, the Hospital Anxiety and Depression Scale(HADS) scores, GABA levels, NSE levels and Glu levels in both groups were significantly lower than those before treatment, and these indicators in the observation group were significantly lower than those in the control group after treatment(P<0.05). After treatment, the time of 10-meter walk test in both groups was significantly shortened compared with that before treatment, and the time of 10-meter walk test in the observation group was significantly shorter than that in the control group after treatment(P<0.05). After treatment, the intramuscular electromyography(iEMG), median frequency(MF), mean power frequency(MPF), Berg Balance Scale(BBS) scores, Fugl-Meyer Assessment(FMA) scale scores, Functional Independence Measure(FIM) scores and modified Barthel Index(MBI) in the two groups were significantly elevated than those before treatment, and these indicators in the observation group were significantly higher than those in the control group after treatment(P<0.05). The total effective rate of the observation group was higher than that of the control group(96.00% vs 82.00%, χ²=5.005, P=0.025). Conclusion　INT is significantly effective in treatment of patients with spastic hemiplegia of lower limbs after stroke. INT can effectively improve the neurological function, lower limb motor function and psychological state of the patients.]]> 2025/10/31 10:05:06 JING Xingquan, CHEN Wanchao, XIE Zhuopeng 75true ［Abstract］　Objective　To explore the diagnostic value of ultra-high b-value apparent diffusion coefficient(ADC) images combined with prostate-specific antigen density(PSAD) for lesions in the prostate central gland region. Methods　A retrospective analysis was conducted on the clinical data of 106 patients with suspected prostate cancer(PCa) and undergoing routine magnetic resonance imaging(MRI) examination for the prostate and diffusion-weighted imaging(DWI) examination with a b-value of 3 000 s/mm² from July 2023 to March 2025 in Nanping First Hospital Affiliated to Fujian Medical University. The lesions were located in the central gland region of the prostate and were scored 3-5 points according to the Prostate Imaging Reporting and Data System(PI-RADS) version 2.1. The mean ADC(ADC_mean), minimum ADC(ADC_min), and relative deviation(RD) were obtained. The levels of prostate-specific antigen(PSA) were detected by using chemiluminescent immunoassay, and PSAD was calculated. Results　Among the 106 patients, 54 patients were diagnosed with central gland prostate cancer(CGPCa )(CGPCa group) by pathological examination, and 52 patients were diagnosed with benign prostatic hyperplasia(BPH)(BPH group) by pathological examination. The levels of ADC_min and ADC_mean in the CGPCa group were lower than those in the BPH group, while the levels of RD, PSA and PSAD in the CGPCa group were higher than those in the BPH group, with statistically significant differences(P<0.05). The results of receiver operating characteristic(ROC) curve analysis showed that ADC_min, ADC_mean, RD, PSA and PSAD could effectively differentiate CGPCa from BPH(P<0.05). The 3 indicators(ADC_min, RD and PSAD) having higher diagnostic efficiency were selected to construct a combined diagnostic model. The results of DeLong′s test showed that the diagnostic efficiency of the combination of the three indicators was higher than that of a single indicator(Z_{three-indicator combination-ADCmin}=1.968, P=0.049; Z_{three-indicator combination-PSAD}=2.081, P=0.037; Z_{three-indicator combination-RD}=1.992, P=0.046), with a sensitivity of 74.07% and a specificity of 100.00%. Conclusion　The diagnostic model constructed on basis of ADC_min, RD and PSAD exhibits significant advantages in differentiating CGPCa from BPH, enhancing the diagnostic efficiency for lesions in the central gland region of the prostate. This model provides a novel methodological strategy for accurately distinguishing CGPCa from BPH and has good clinical application value.]]> 2025/10/31 0:00:00 YE Fei, HUANG Honglei, FAN Zhengrong, LI Feng 74true ［Abstract］　Objective　To study the mechanisms of long non-coding ribonucleic acid(RNA)(lncRNA) small nucleolar RNA host gene 16(SNHG16) promoting breast cancer radioresistance by binding to host cellular RNA binding protein Staufen 1(STAU1) to reduce the stability of Parkin RBR E3 ubiquitin protein ligase(PRKN) mRNA. Methods　The expressions of lncRNA SNHG16 in breast cancer tissues were analyzed by using The Cancer Genome Atlas(TCGA) database and real-time quantitative polymerase chain reaction(RT-qPCR) was used to detect the expressions of lncRNA SNHG16 in breast cancer cells. MDA-MB-231 and MCF-7 cells with high expression of lncRNA SNHG16 were selected for transfection and were divided into si-NC group(control group) and si-SNHG16 group(experimental group). Cells in logarithmic growth phase were taken for experiment 48 hours after transfection. Cell proliferation was detected by using Cell Counting Kit-8(CCK-8) assay. Cell apoptosis was detected by using flow cytometry. Cell invasion was detected by using Transwell assay. Cell radiosensitivity was detected by using CCK-8 assay. Cellular glucose uptake was determined by using the glucose uptake colorimetric assay kit. The combination of RNA immunoprecipitation(RIP) detection with RNA stability experiment was used to verify the regulatory relationship among lncRNA SNHG16, STAU1 and PRKN. Results　lncRNA SNHG16 was highly expressed in breast cancer tissues and cells. Knockdown of lncRNA SNHG16 expression reversed the proliferation, apoptosis, invasion and radioresistance of the breast cancer cells, and inhibited the reprogramming of glucose metabolism in breast cancer cells. The RIP experiment confirmed that there was an interaction between STAU1 and PRKN mRNA, whereas knockdown of lncRNA SNHG16 attenuated this interaction relationship, and knockdown of STAU1 expression also enhanced the stability of PRKN mRNA. Conclusion　The highly expressing lncRNA SNHG16 in breast cancer cells binding to STAU1 reduces the stability of PRKN mRNA via a post-transcriptional modification pathway, thus affecting the reprogramming of glucose metabolism and promoting radioresistance in breast cancer.]]> 2025/10/31 0:00:00 1, CHEN Ru²]]> ZHONG Rui¹, CHEN Ru² 73true ［Abstract］　Objective　To explore the application value of time-lapse imaging technology in monitoring embryonic direct cleavage(DC) and evaluating embryonic developmental potential. Methods　The clinical data of patients who received in vitro fertilization(IVF) or intracytoplasmic sperm injection(ICSI) treatment in Reproductive Medicine and Genetics Center, the People′s Hospital of Guangxi Zhuang Autonomous Region from July 2020 to February 2024 were retrospectively analyzed, with a total of 601 treatment cycles. The development process of embryos was monitored by using time-lapse imaging technology. According to the embryonic cleavage patterns, the embryos were divided into normal cleavage group and DC group. According to the periods of DC, the DC group was further divided into DC1 group(DC occurred in the first division of the fertilized eggs), DC2 group(DC occurred in the second division of the fertilized eggs) and DC3 group(DC occurred in the third division of the fertilized eggs). The indicators of embryonic developmental potential and pregnancy outcomes were compared among the groups. Results　The monitoring results of time-lapse imaging showed that the incidence of DC in the embryos was 16.67%(822/4 932). The mean of blastomeres of D3 embryos in the DC group was significantly less than that in the normal cleavage group, and the rates of D3 high-quality embryos, D6 blastocysts formation, D6 high-quality blastocyst formation and D6 freezable blastocyst formation in the DC group were significantly lower than those in the normal cleavage group(P<0.05). The D3 high-quality embryo rates in the DC1 group and the DC2 group were significantly lower than those in the normal cleavage group(P<0.05). The D3 high-quality embryo rates in the DC1 group were significantly lower than those in the DC2 group and the DC3 group(P<0.05). The D6 blastocyst formation rates in the DC1 group and the DC2 group were significantly lower than those in the normal cleavage group(P<0.05). The D6 blastocyst formation rates in the DC1 group were significantly lower than those in the DC2 group and the DC3 group(P<0.05). The D6 high-quality blastocyst formation rates in the DC1 group, the DC2 group and the DC3 group were significantly lower than those in the normal cleavage group(P<0.05). The D6 high-quality blastocyst formation rates in the DC1 group were significantly lower than those in the DC2 group and the DC3 group(P<0.05). Compared with those in the normal cleavage group, the biochemical pregnancy rate, clinical pregnancy rate, implantation rate and live birth rate in the DC1 group and the DC2/3 group were significantly decreased(P<0.05). Conclusion　DC can reduce the embryonic development potential, and the earlier DC occurs, the greater the negative impact on the embryonic development. Time-lapse imaging technology is an effective method to monitor the occurrence of DC in the embryos and evaluate the embryonic development potential.]]> 2025/10/31 10:05:06 1, PAN Yongyi¹, WANG Shikai², ZHANG Xiaohui², WEI Pingpin², LI Zhengda², MAO Xianbao², CHEN Liangshi², XUE Lintao^1,2]]> LIU Haifang¹, PAN Yongyi¹, WANG Shikai², ZHANG Xiaohui², WEI Pingpin², LI Zhengda², MAO Xianbao², CHEN Liangshi², XUE Lintao^1,2 72true ［Abstract］　Objective　To analyze the factors influencing poor clinical outcomes in patients with acute hypoxemic respiratory failure(AHRF) after Venturi mask oxygen therapy and to construct a nomogram model. Methods　A retrospective analysis was conducted on the clinical data of 203 patients with AHRF who were admitted to Anqing Hospital of Chinese Medicine from November 2021 to April 2024. All these patients received Venturi mask oxygen therapy. According to the clinical outcomes of the patients after the therapy, they were divided into poor outcome group(57 patients) and good outcome group(146 patients). Multivariate logistic regression was used to analyze the factors influencing the clinical outcomes of patients with AHRF after Venturi mask oxygen therapy, and a nomogram model for predicting poor clinical outcomes of patients with AHRF after Venturi mask oxygen therapy was constructed based on the screened indicators. The predictive efficiency and application value of the constructed nomogram model were evaluated through receiver operating characteristic(ROC) curve, Hosmer-Lemeshow test, calibration curve and decision curve. Results　In the 203 patients, 57 patients(28.08%) had poor clinical outcomes. Among the 57 patients with poor clinical outcomes, 45 patients were converted to mechanical ventilation, and 10 patients had deteriorated conditions leading to rescue, and 2 patients died. The results of multivariate logistic regression analysis showed that older age［OR(95%CI)=1.754(1.040-2.958)］, higher Acute Physiology and Chronic Health Evaluation Ⅱ(APACHE Ⅱ) scores［OR(95%CI)=2.373(1.535-3.669)］, fever［OR(95%CI)=2.274(1.170-4.419)］ and concurrent multiple organ dysfunction syndrome(MODS)［OR(95%CI)=1.635(1.093-2.446)］ were independent risk factors for poor clinical outcomes in patients with AHRF after Venturi mask oxygen therapy(P<0.05). A high respiratory rate-oxygenation(ROX) index at 1 hour after treatment［OR(95%CI)=0.556(0.364-0.849)］ was an independent protective factor for promoting good clinical outcomes in the patients(P<0.05). A nomogram model for predicting poor clinical outcomes in patients with AHRF after Venturi mask oxygen therapy was constructed on basis of age, fever, concurrent MODS, APACHE Ⅱ scores and ROX index at 1 hour after treatment. The results of ROC curve analysis showed that the nomogram model had good predictive efficiency［AUC(95%CI)=0.925(0.880-0.957), P<0.001］, with a sensitivity of 92.98% and a specificity of 85.62%. The results of Hosmer-Lemeshow test showed that there was no statistically significant difference between the probability of the patients′ poor clinical outcomes predicted by the nomogram model and the actual probability(χ²=0.859, P=0.354). The calibration curve showed that the predicted probability of the nomogram model had a good consistency with the actual probability. The decision curve indicated that the nomogram model could be applied to the vast majority of clinical decision-making settings. Conclusion　Age, fever, concurrent MODS, APACHE Ⅱ scores, and the ROX index at 1 hour after treatment are the factors influencing the clinical outcomes of AHRF patients after Venturi mask oxygen therapy. The nomogram model constructed on basis of these indicators is helpful for rapid clinical decision-making, improves the patients′ prognosis, and has good clinical application value.]]> 2025/10/31 10:05:07 1, FANG Hui¹, XIA Yunqing²,HU Ai³, LI Bisheng⁴, WANG Lingling¹, WANG Rui¹, WANG Bin¹]]> JIANG Long¹, FANG Hui¹, XIA Yunqing²,HU Ai³, LI Bisheng⁴, WANG Lingling¹, WANG Rui¹, WANG Bin¹ 71true ［Abstract］　Objective　To analyze the clinical phenotypes and genetic variation characteristics of 21 pediatric patients with neonatal seizures of unknown causes. Methods　A retrospective analysis was conducted on the clinical data of 21 pediatric patients with neonatal seizures of unknown causes who were admitted to Department of Neonatology, the First Affiliated Hospital of Xinxiang Medical University and Department of Neonatology, Luoyang Maternal and Child Health Hospital from September 2018 to October 2024. The results of blood tandem mass spectrometry, urine gas chromatography-mass spectrometry and whole exome gene detection were analyzed, and the clinical outcomes of the pediatric patients were summarized. Results　In the 21 pediatric patients, 7 pediatric patients were male and 14 pediatric patients were female. All of the pediatric patients presented with seizures as the initial manifestation. There were no abnormalities in the blood tandem mass spectrometry and urine gas chromatography-mass spectrometry. Head magnetic resonance imaging(MRI) indicated that there were no abnormalities or the abnormal results were not specific. The results of 24-hour video electroencephalography(EEG) in all the pediatric patients showed abnormalities, mainly including sharp waves, spike waves and slow waves, with a few abnormalities showing burst-inhibition. The genetic testing results in the 21 pediatric patients were positive. There were 7 cases of benign familial neonatal seizures caused by variations in KCNQ2, KCNQ3 and SCN2A genes, and 12 cases of developmental and epileptic encephalopathies caused by variations in KCNA2, KCNQ2, CNPY3, ATP6V1A and MT-ND3 genes. Two pediatric patients with variations in KCNQ2 gene were lost to follow-up and their clinical phenotypes could not be determined. The follow-up was conducted until April 2025. Two pediatric patients were lost to follow up. Seven pediatric patients died, all of whom were pediatric patients with developmental and epileptic encephalopathies. Conclusion　Genetic testing is helpful for judging the prognosis of neonatal seizures. This study reveals that variations in CNPY3 and ATP6V1A genes lead to developmental and epileptic encephalopathies, which enriches the disease gene spectrum.]]> 2025/10/31 0:00:00 1, ZHAO Haihai¹, WANG Weiwei¹, LI Zhenzhen¹, SHANG Yun¹, SUN Yazhou¹, HUANG Shuixia²]]> CUI Qingyang¹, ZHAO Haihai¹, WANG Weiwei¹, LI Zhenzhen¹, SHANG Yun¹, SUN Yazhou¹, HUANG Shuixia² 70true ［Abstract］　Objective　To observe the application effect of neurodevelopmental therapy combined with cognitive training in the family rehabilitation treatment for infants with global developmental delay(GDD). Methods　Fifty infants with GDD who were admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2019 to December 2020 were recruited and divided into observation group(receiving neurodevelopmental therapy combined with cognitive training) and control group(receiving neurodevelopmental therapy) by random number table method, with 25 infants in each group. In the control group, 3 infants were excluded due to their incomplete treatments, and finally 25 infants were included in the observation group and 22 infants in the control group. The development quotients(DQ) of the GDD infants′ adaptation functional domain, gross motor functional domain, fine motor functional domain, language functional domain and personal social contact functional domain were evaluated by using Gesell Developmental Schedules before treatment, at 1 month of treatment and at 3 months of treatment, and comparisons were conducted between the two groups. Results　There were no significant differences in the DQ of the adaptation functional domain, gross motor functional domain, fine motor functional domain, language functional domain and personal social contact functional domain between the two groups before treatment(P>0.05). At 1 month and 3 months of treatment, the DQ of the adaptation functional domain, gross motor functional domain, fine motor functional domain and language functional domain in the two groups were significantly increased compared with those before treatment(P<0.05), and the DQ of the 4 functional domains in the observation group were higher than those in the control group at 3 months of treatment, and the differences were statistically significant(P<0.05). At 3 months of treatment, the DQ of personal social contact functional domain in the two groups were significantly increased compared with those before treatment(P<0.05), and the DQ in the observation group were higher than those in the control group at 3 months of treatment, and the differences were statistically significant(P<0.05). Conclusion　In family rehabilitation treatment, the application of neurodevelopmental therapy combined with cognitive training can improve the development levels of the cognitive, motor and language functional domains in GDD infants.]]> 2025/10/31 10:05:07 LI Liewang, HE Shuang, LIU Yuancheng, XIAN Jun, LI Wei 69true ［Abstract］　Objective　To analyze the 90% effective dose(ED₉₀) of midazolam for oral mucosal administration for sedation during head magnetic resonance imaging(MRI) examination in infants weighing less than 5 kg. Methods　The infants who underwent head MRI examination under sedation in Maternity and Child Health Care of Guangxi Zhuang Autonomous Region from March 2024 to October 2024 were recruited. Sedative intervention was conducted on the included infants in sequence using a biased-coin design method. The first infant received midazolam oral solution at a dose of 0.15 mg/kg through oral mucosal route of administration. The dose for each subsequent infant was determined based on the sedation outcome of the previous infant: (1)If the previous infant experienced sedation failure, the current infant received midazolam oral solution at a dose increased by 1 unit(0.05 mg/kg) through oral mucosal route of administration compared to the previous dose given to the the previous infant. (2)If the previous infant achieved success of sedation, the dose for the current infant was either maintained(with 89% probability) or reduced by 1 unit(with 11% probability) using a biased-coin design method. The trial was terminated when the number of successful sedation cases reached 45. The ED₉₀ was calculated, and isotonic regression with bootstrap resampling was employed to estimate the 95% effective dose(ED₉₅) and the 99% effective dose(ED₉₉). Results　A total of 53 infants were included in this study, among whom 45 infants were successfully sedated and 8 infants failed to be sedated. The ED₉₀ of midazolam administered via the oral mucosal route for sedation during MRI examination in the infants weighing less than 5 kg was 0.295 mg/kg(95%CI=0.236-0.335 mg/kg). After calculation using isotonic regression and bootstrap resampling, the ED₉₅ was estimated to be 0.325 mg/kg(95%CI=0.289-0.345 mg/kg) and the ED₉₉ was estimated to be 0.347 mg/kg(95%CI=0.340-0.364 mg/kg). Conclusion　The ED₉₀ of midazolam administered via oral mucosal route for sedation during head MRI examination in infants weighing less than 5 kg is 0.295 mg/kg(95%CI=0.236-0.335 mg/kg).]]> 2025/10/31 0:00:00 LAN Jiangli, ZHENG Jianqiu, DENG Lingyan, CHEN Ying, HONG Wei, PAN Lixia, FENG Jifeng 68true ［Abstract］　Objective　To analyze the efficacy of levosimendan in treatment of patients with acute decompensated heart failure(ADHF) assessed by using critical care ultrasound. Methods　A retrospective analysis was conducted on the clinical data of 156 patients with ADHF who were admitted to the Department of Cardiology of the People′s Hospital of Guangxi Zhuang Autonomous Region from September 2020 to December 2021. According to whether the patients received levosimendan treatment during hospitalization, they were divided into levosimendan group(74 patients) and control group(82 patients). The changes in plasma N-terminal pro-B-type natriuretic peptide(NT-proBNP), left ventricular end-diastolic diameter(LVEDD), left atrial diameter(LAD), left ventricular ejection fraction(LVEF), and lung ultrasound B-line counts before and after the treatment were compared between the two groups. The in-hospital mortality rate, all-cause mortality rates within 1 month and within 6 months after discharge, and readmission rate due to heart failure(HF) within 6 months after discharge were compared between the two groups. Multivariate Cox regression was used to analyze the relationship of levosimendan treatment and lung ultrasound B-line counts with primary endpoint events. Results　Compared with those before treatment, NT-proBNP decreased, and LVEF increased, and lung ultrasound B-line counts decreased in both groups after treatment, and LVEDD in the levosimendan group decreased after treatment, with statistically significant differences(P<0.05). After treatment, the degree of decrease in NT-proBNP and the degree of decrease in lung ultrasound B-line counts in the levosimendan group were significantly greater than those in the control group(P<0.05). The readmission rate due to HF within 6 months after discharge in the levosimendan group was significantly lower than that in the control group(P<0.05). The results of multivariate Cox regression analysis showed that the risk of readmission due to HF within 6 months after discharge in the levosimendan group was significantly lower than that in the control group(P<0.05), and the lung ultrasound B-line counts ≥3 could increase the risk of readmission due to HF within 6 months after discharge(P<0.05). Conclusion　The combination of conventional treatment and levosimendan can more significantly improve the cardiac function of ADHF patients, reduce pulmonary congestion, and lower the readmission rate due to HF within 6 months after discharge, without increasing short-term all-cause mortality and the risk of all-cause mortality. Critical care ultrasound assessment can provide important objective basis for the treatment decision-making and prognostic judgment in ADHF, and is worthy of clinical promotion.]]> 2026/7/31 10:41:33 1, Ji Qingwei¹, Liu Ling¹, Shi Ying¹, Huang Zongyan¹, Shen Jie², Yang Fan³]]> Lin Jing¹, Ji Qingwei¹, Liu Ling¹, Shi Ying¹, Huang Zongyan¹, Shen Jie², Yang Fan³ 67true ［Abstract］　Objective　To analyze the risk factors of adverse functional outcomes in intracerebral hemorrhage(ICH) patients complicated with pulmonary embolism(PE), aiming to facilitate early identification of high-risk individuals and improve the patients′ prognosis. Methods　A retrospective analysis was conducted on the clinical data of 152 adult patients with acute spontaneous ICH complicated with PE who were admitted to the First Affiliated Hospital of Guangxi Medical University from January 2013 to December 2024. According to the modified Rankin Scale(mRS) scores at discharge, the patients were divided into a good functional outcome group(mRS scores of 0-2 points, n=43) and an adverse functional outcome group(mRS scores of 3-6 points, n=109). Multivariate logistic regression was used to analyze the influencing factors of adverse functional outcomes in the ICH patients complicated with PE. Results　Among the 152 patients, the in-hospital mortality rate was 9.21%, and 109 patients(71.71%) had adverse functional outcomes at discharge. Compared with the good functional outcome group, the adverse functional outcome group had higher proportions of patients with hypertension, hemiplegia, coma, intraventricular hemorrhage(IVH), external ventricular drainage and anticoagulation therapy, and had longer time spent bedridden, higher levels of systemic inflammatory response index(SIRI), D-dimer when diagnosed with PE, dynamic changing value 1 of D-dimer(ΔD-dimer₁=D-dimer level when diagnosed with PE-D-dimer level at admission) and systemic immune-inflammation index(SII), and a lower proportion of patients receiving decompressive craniectomy, shorter activated partial thromboplastin time(APTT), lower mean corpuscular hemoglobin concentration(MCHC), with statistically significant differences(P<0.05). The results of multivariate logistic regression analysis showed that IVH, higher ΔD-dimer₁ and SIRI levels were risk factors of adverse functional outcomes in ICH patients complicated with PE(P<0.05). Conclusion　IVH, higher ΔD-dimer₁ and SIRI levels are independent risk factors of adverse functional outcomes in ICH patients complicated with PE.]]> 2026/7/31 10:41:33 Xu Lishan, Wei Hong, Wang Miao, Huang Xiulin, Liang Zhijian 66true ［Abstract］　Objective　To observe the efficacy of ventriculoperitoneal long-tunneled external drainage in treatment of postoperative intracranial infection complicated with acute hydrocephalus. Methods　A retrospective analysis was conducted on the clinical data of 80 patients with postoperative intracranial infection complicated with acute hydrocephalus following surgery for acute brain injury who were admitted to the Second People＇s Hospital of Hunan Province(Brain Hospital of Hunan Province) from January 2022 to June 2025. Among the 80 patients, 32 patients were treated with ventriculoperitoneal long-tunneled external drainage(observation group), and the other 48 patients were treated with conventional external ventricular drainage via frontal horn puncture(control group). Perioperative indicators and prognostic indicators were compared between the two groups. Results　The single drainage duration and single operation duration in the observation group were longer than those in the control group. The total number of operations in the observation group was less than that in the control group. The incidence rates of secondary intracranial infection, cerebrospinal fluid leakage at the incision and catheter displacement in the observation group were lower than those in the control group.The above differences were statistically significant between the two groups(P<0.05). There were no statistically significant differences between the two groups in the improvement rate of hydrocephalus, modified Rankin Scale(mRS) scores, the treatment rate of ventriculoperitoneal shunt(VPS) and mortality rate at 3 months postoperatively(P>0.05). Conclusion　For patients with obstructive hydrocephalus who require long-term drainage catheter indwelling while awaiting complete control of intracranial infection, ventriculoperitoneal long-tunneled external drainage can prolong drainage duration, reduce the number of operations, reduce the incidence rates of secondary intracranial infection, cerebrospinal fluid leakage at the incision and catheter displacement, and therefore is an effective supplementary treatment approach.]]> 2026/7/31 0:00:00 1, Du Hao¹, Li Xin²]]> Chen Jiru¹, Du Hao¹, Li Xin² 65true ［Abstract］　Objective　To construct a nomogram model for diagnosing severe Mycoplasma pneumoniae pneumonia(SMPP) in children and to analyze the diagnostic efficacy of the model. Methods　A retrospective analysis was conducted on the clinical data of 966 pediatric patients with Mycoplasma pneumoniae pneumonia(MPP) who were admitted to the First Teaching Hospital of Xinjiang Medical University from May 2023 to May 2024. The pediatric patients were randomly divided into a training set(680 patients) and a validation set(286 patients) at a ratio of 7∶3. According to the severity of MPP, the pediatric patients were divided into SMPP group and non-SMPP group. Univariate analysis, Lasso regression analysis and multivariate logistic regression analysis were performed on the training set data to screen variables with diagnostic significance for SMPP and a nomogram model was constructed based on these variables. The diagnostic accuracy and clinical application value of the nomogram model were evaluated through receiver operating characteristic(ROC) curve analysis, calibration curve analysis and decision curve analysis(DCA) using the training set and validation set data. Results　A total of 12 significant indicators were obtained through Lasso regression analysis: age, fever, decreased breath sounds, pulmonary complications(overall), pleural effusion, neutrophil-to-lymphocyte ratio(NLR), platelet-to-lymphocyte ratio(PLR), C-reactive protein(CRP), erythrocyte sedimentation rate(ESR), lactate dehydrogenase(LDH), bronchial inflation sign, and lesion site in the lower left lobe. The results of multivariate logistic regression analysis showed that age(OR=1.102), decreased breath sounds(OR=4.714), pulmonary complications(OR=4.740), LDH(OR=1.012) and ESR(OR=1.053) were independent influencing factors for the occurrence of SMPP(P<0.05). The results of ROC curve analysis showed that the constructed nomogram model could effectively differentiate between SMPP and MPP［training set: AUC(95%CI)=0.801(0.767-0.834); validation set: AUC(95%CI)=0.799(0.746-0.851), P<0.05］. The results of calibration curve analysis indicated that the nomogram model had satisfactory goodness of fit and high diagnostic accuracy. The results of DCA showed that the nomogram model had a high net benefit rate in diagnosing SMPP and has good clinical practical value. Conclusion　The nomogram model constructed based on the indicators of age, decreased breath sounds, pulmonary complications, LDH and ESR has high accuracy in diagnosing SMPP in children, which is helpful for early clinical identification of the children at high risk of SMPP and for taking corresponding preventive and therapeutic measures to improve their prognosis.]]> 2026/7/31 10:41:33 1, Ru Liang²]]> Wang Yingru¹, Ru Liang² 64true ［Abstract］　Objective　To explore the effects of abdominal electrical stimulation combined with diaphragm pacing therapy device intervention on airway clearance ability and extubation success rate in patients with tracheotomy after cerebral trauma. Methods　Eighty patients with cerebral trauma who were admitted to Zhongda Hospital Lishui Branch, Southeast University from August 2021 to June 2025 were recruited and underwent tracheotomy. The patients were divided into observation group and control group by using random number table method, with 40 patients in each group. The control group received intervention with a diaphragm pacing therapy device, while the observation group received abdominal electrical stimulation plus intervention with a diaphragm pacing therapy device. Both groups were intervened for 2 weeks. The indicators of cough and sputum expulsion ability, diaphragm ultrasound and pulmonary ventilation function, as well as the scores of Generic Quality of Life Inventory-74(GQOLI-74), Mini-Mental State Examination Scale(MMSE) and Glasgow Outcome Scale(GOS) were compared between the two groups before and after the intervention. The extubation time, extubation success rate and length of hospital stay were compared between the two groups. The occurrence of treatment-related adverse reactions was recorded. Results　After the intervention, the involuntary cough peak flow(ICPF) and Semi-Quantitative Cough Strength Score(SCSS) were increased while the amount of sputum discharge was increased in both groups compared with those before the intervention, and the levels of these indicators in the observation group were higher than those in the control group after the intervention, with statistically significant differences(P<0.05). After the intervention, the levels of diaphragmatic excursion, diaphragmatic thickness, diaphragmatic thickening fraction, forced expiratory volume in 1 second(FEV₁), peak expiratory flow(PEF), forced vital capacity(FVC), and maximum ventilation volume(MVV) in both groups were higher than those before the intervention, and the levels of these indicators in the observation group were higher than those in the control group after the intervention, with statistically significant differences(P<0.05). The time for extubation in the observation group was earlier than that in the control group, and the success rate of extubation in the observation group was higher than that in the control group, and the length of hospital stay in the observation group was shorter than that in the control group, with statistically significant differences(P<0.05). After the intervention, the scores of GQOLI-74, MMSE and GOS in both groups were higher than those before the intervention, and these scores in the observation group were higher than those in the control group after the intervention, with statistically significant differences(P<0.05). During the treatment period, no treatment-related adverse reactions occurred in either group. Conclusion　Abdominal electrical stimulation plus diaphragm pacing therapy device intervention is safe and effectively improves the airway clearance ability, and increases the success rate of extubation in patients with tracheotomy after cerebral trauma.]]> 2026/7/31 10:41:33 1, Sun Yunlei¹, Hou Lingxian¹, Tang Congzhi²]]> Liu Ting¹, Sun Yunlei¹, Hou Lingxian¹, Tang Congzhi² 63true ［Abstract］　Objective　To observe clinical outcomes of arm-reduced robot-assisted laparoscopic radical resection in males with low rectal cancer. Methods　The clinical data of 186 male patients with low rectal cancer who were admitted to the Department of Gastrointestinal Surgery of Nanxishan Hospital of Guangxi Zhuang Autonomous Region from October 2021 to May 2025 were retrospectively analyzed. The robot-assisted laparoscopic radical resection of low rectal cancer(Dixon) plus prophylactic ileostomy were successfully completed in all the patients. According to the surgical approaches, the patients were divided into the conventional group(receiving conventional robot-assisted laparoscopic radical resection of low rectal cancer, 81 patients) and the arm-reduced group(receiving arm-reduced robot-assisted laparoscopic radical resection of low rectal cancer, 105 patients). The perioperative indicators, hospitalization expenses, occurrence of complications, as well as postoperative defecation function and urination function were compared between the two groups. Results　The hospitalization expenses of the arm-reduced group were lower than those of the conventional group, with statistically significant differences(P<0.05). There were no statistically significant differences in the operative duration, intraoperative blood loss, time to first flatus, number of harvested lymph nodes and length of postoperative hospital stay between the two groups(P>0.05). There was no statistically significant difference in the total incidence of postoperative complications between the two groups(P>0.05). A total of 93 patients in the arm-reduced group and 75 patients in the conventional group underwent scheduled ileostomy reversal and were successfully discharged from the hospital. There were no statistically significant differences in International Prostate Symptom Scale(IPSS) scores and low anterior resection syndrome(LARS) scores between the two groups(P>0.05). Conclusion　The clinical outcomes of arm-reduced robot-assisted laparoscopic radical resection are comparable to those of conventional robot-assisted laparoscopic radical resection in treatment of low rectal cancer in male patients. The arm-reduced robot-assisted laparoscopic radical resection does not increase the risk of adverse events, and has more economic advantages, and therefore is worthy of clinical recommendation.]]> 2026/7/31 10:41:33 Qin Cheng, Guo Jie, Yang Tao, Qi Junjun, Zhang Qiaowen, Li Chuijin, Pan Xiulei, He Guoli, Liu Lihua 62true ［Abstract］　Objective　To analyze the value of serum homeobox A10(HOXA10), granulocyte-macrophage colony-stimulating factor(GM-CSF) and cytoplasmic polyadenylation element binding protein 1(CPEB1) for predicting pregnancy outcomes after in vitro fertilization-embryo transfer(IVF-ET) in polycystic ovary syndrome(PCOS) patients complicated with infertility. Methods　A total of 125 PCOS patients complicated with infertility who received IVF-ET in Yellow River Sanmenxia Hospital from November 2022 to July 2024 were recruited as the research subjects. The patients′ fasting venous blood was collected before initiation of the IVF-ET cycle(before downregulation). Sex hormone levels were detected by using an automated biochemical immunoanalyzer. Serum levels of HOXA10, GM-CSF and CPEB1 were detected by using enzyme-linked immunosorbent assay(ELISA). Transvaginal ultrasound examination was performed to assess pregnancy outcomes 4 weeks after IVF-ET. According to the pregnancy outcomes, the patients were divided into pregnancy group(58 patients) and pregnancy failure group(67 patients). Multivariate logistic regression was used to analyze the factors influencing the pregnancy outcomes. The predictive efficacy of the indicators for pregnancy outcomes was analyzed by using receiver operating characteristic(ROC) curves. Results　Compared with those in the pregnancy group, the levels of estradiol(E₂) and GM-CSF in the pregnancy failure group were lower, while the levels of testosterone(T), HOXA10 and CPEB1 in the pregnancy failure group were higher, with statistically significant differences between the two groups(P<0.05). The results of multivariate logistic regression analysis showed that higher levels of HOXA10 and CPEB1 were risk factors for pregnancy failure in the PCOS patients complicated with infertility after IVF-ET(P<0.05), and a higher GM-CSF level was a protective factor for pregnancy failure in the PCOS patients complicated with infertility after IVF-ET(P<0.05). The results of ROC curve analysis showed that the levels of HOXA10, GM-CSF and CPEB1 could effectively predict pregnancy failure in the PCOS patients complicated with infertility after IVF-ET(P<0.05). Moreover, the predictive efficacy of the combined detection of the three indicators［AUC(95%CI)=0.944(0.888-0.977)］ was better than that of a single indicator(P<0.05). Conclusion　Serum levels of HOXA10, CPEB1 and GM-CSF are the influencing factors of pregnancy outcomes in the PCOS patients complicated with infertility after IVF-ET. The combined detection of the three indicators has a high predictive value for pregnancy outcomes.]]> 2026/7/31 10:41:33 Ji Wenwen, Tian Ying, Cao Zelei 61true ［Abstract］　Objective　To construct a nomogram model for differentiating Gleason grades in prostate cancer(PCa) based on multiparametric magnetic resonance imaging(mpMRI) radiomics and clinicopathological characteristics, and to evaluate the diagnostic efficacy and application value of the model. Methods　A total of 180 patients with PCa who were admitted to Suining County People′s Hospital from January 2024 to December 2024 were recruited. According to the results of pathological examination and Gleason grades of the patients, they were divided into low-grade PCa group(88 patients, with Gleason grades of 1-2) and high-grade PCa group(92 patients, with Gleason grades of 3-5). All the patients underwent magnetic resonance imaging(MRI) examination, and their mpMRI radiomics features were extracted. Ultimately, 2 optimal features were selected from each of the T₂-weighted imaging(T₂WI) and diffusion-weighted imaging(DWI) sequences, and these 4 indicators were used to construct an mpMRI radiomics scoring system. Multivariate logistic regression analysis was used to screen the indicators that were helpful for differentiating high-grade PCa from low-grade PCa, and a nomogram model was constructed based on the screened indicators. The diagnostic efficacy and application value of the nomogram model were analyzed by using receiver operating characteristic(ROC) curve, calibration curve and decision curve. Results　The mpMRI radiomics scores, maximum diameter of tumors, prostate specific antigen(PSA), prostate specific antigen density(PSAD), the proportion of T stages of T_3-4 and the proportion of N stage of N₁ in the high-grade PCa group were significantly larger than those in the low-grade PCa group(P<0.05). The results of multivariate logistic regression analysis showed that mpMRI radiomics scores, maximum diameter of tumors, PSA, PSAD and N stage were the meaningful indicators for distinguishing the high-grade PCa from the low-grade PCa(P<0.05), and a nomogram model was constructed based on the 5 indicators. The results of ROC curve analysis showed that the nomogram model had good efficacy of differential diagnosis［AUC(95%CI)= 0.996(0.972-0.999)］. The calibration curve was close to the ideal state, indicating that the diagnostic accuracy of the nomogram model was good. The results of decision curve analysis showed that within the predicted probability ranging from 2% to 100%, the nomogram model could provide better clinical net benefits. Conclusion　The nomogram model constructed based on mpMRI radiomics and clinicopathological features has good diagnostic efficacy and clinical practical value for the differential diagnosis of high-grade PCa and low-grade PCa.]]> 2026/7/31 10:41:34 1, Xie Lixiang², Zhuo Shuo², Zhang Zehua¹, Li Shaodong²]]> Song Tengteng¹, Xie Lixiang², Zhuo Shuo², Zhang Zehua¹, Li Shaodong² 60true ［Abstract］　Objective　To analyze the correlations of serum activated leukocyte cell adhesion molecule(ALCAM), B7 homolog 4(B7-H4) and S100A4 levels with disease severity and postoperative recurrence in chronic rhinosinusitis with nasal polyps(CRSwNP) patients. Methods　The clinical data of 173 patients with chronic rhinosinusitis(CRS) who were admitted to the First People′s Hospital of Lin′an District, Hangzhou from January 2020 to December 2022 were respectively analyzed and divided into CRSwNP group(100 patients) and chronic rhinosinusitis without nasal polyps(CRSsNP) group(73 patients) according to whether they had nasal polyps or not. The patients with CRSwNP received sinus computed tomography(CT) examination before surgery, and Lund-Mackay scores were evaluated based on the results of the CT examination: 0-8 points for mild lesions, 9-16 points for moderate lesions, and 17-24 points for severe lesions. The levels of ALCAM, B7-H4 and S100A4 in the serum of the patients were detected by using enzyme-linked immunosorbent assay(ELISA). The CRSwNP patients received functional endoscopic sinus surgery. The patients were followed up for 1 year and the recurrence of their diseases was observed. Pearson correlation was used to analyze the correlations of serum ALCAM, B7-H4 and S100A4 levels with preoperative Lund-Mackay scores. Multivariate logistic regression was applied to analyze the factors influencing postoperative recurrence in patients with CRSwNP. The receiver operating characteristic(ROC) curve was applied to analyze the efficacy of serum ALCAM, B7-H4 and S100A4 levels in predicting postoperative recurrence in patients with CRSwNP. Results　Forty-eight cases of mild lesions, 33 cases of moderate lesions, and 19 cases of severe lesions in CRSwNP were included in this study. The levels of serum ALCAM, B7-H4 and S100A4 in the CRSwNP group were significantly higher than those in the CRSsNP group(P<0.05). The levels of serum ALCAM, B7-H4 and S100A4 in patients with moderate and severe lesions in CRSwNP were higher than those in patients with mild lesions in CRSwNP, and the levels of these indicators in patients with severe lesions were higher than those in patients with moderate lesions, with statistically significant differences(P<0.05). The results of Pearson correlation analysis showed that the serum ALCAM level(r=0.436, P=0.006), B7-H4 level(r=0.528, P<0.001), and S100A4 level(r=0.561, P<0.001) in the CRSwNP patients were positively correlated with the preoperative Lund-Mackay scores. Among the 100 patients with CRSwNP, 36 patients experienced recurrence within 1 year after surgery. The results of multivariate logistic regression analysis showed that higher levels of serum ALCAM, B7-H4 and S100A4 were independent risk factors for postoperative recurrence in the CRSwNP patients(P<0.05). The results of ROC curve analysis showed that the combined detection of serum ALCAM, B7-H4 and S100A4 had better efficacy in predicting postoperative recurrence in patients with CRSwNP than the detection of a single indicator(Z_{three-indicator-combined-ALCAM}=2.840, P=0.005; Z_{three-indicator-combined-B7-H4}=2.736, P=0.006; Z_{three-indicator-combined-S100A4}=2.645, P=0.008), with a sensitivity of 97.22% and a specificity of 84.37%. Conclusion　The levels of serum ALCAM, B7-H4 and S100A4 in CRSwNP patients are positively correlated with the severity of lesions, and higher levels of these indicators are associated with the patients′ postoperative recurrence. These findings assists clinicians in identifying patients with higher recurrence risk as early as possible.]]> 2026/7/31 10:41:34 Cai Dongbo, Zhang Feng, Liu Yongsheng, Wei Liang 59true ［Abstract］　Objective　To construct a nomogram model for predicting cognitive impairment in older adults based on their dietary patterns. Methods　The data were selected from the 2018 Chinese Longitudinal Healthy Longevity Survey(CLHLS), and the participants were adults aged 65 years and older. Dietary patterns were calculated based on the consumption frequency of food items collected through face-to-face interviews. Cognitive function was assessed by using the Mini-Mental State Examination(MMSE) scale. A total of 6 681 participants who met the criteria were randomly divided into a training set and a validation set at a ratio of 7∶3 using the statistical software R package “caret”(4.6.1), with 4 677 participants in the training set and 2 004 participants in the validation set. Based on the training set data, logistic regression analysis were conducted to screen independent predictors and construct a nomogram model. Receiver operating characteristic(ROC) curve, calibration curve and decision curve analysis(DCA) were used to assess the predictive efficacy and clinical applicability of the nomogram model. Results　Twelve independent influencing factors of cognitive impairment in the older adults were screened out by a multivariate logistic regression model, including age, gender, staple food type, vegetables, fish, eggs, dairy products, nuts, seaweed, vitamin supplements, alcohol consumption and body mass index(BMI). Based on these influencing factors, a nomogram model was constructed. The nomogram showed good discriminative ability, and the area under the curve(AUC) in the training set and the validation set was 0.782 and 0.736, respectively. The calibration curve showed a good agreement between the predicted probabilities and the actual observed values. The DCA showed that within the threshold probabilities ranging from 5% to 80%, the net benefit of the model was better than that of “all the interventions” and that of “no interventions” strategies, proving that the nomogram model had good clinical practical value. Conclusion　The nomogram model has certain clinical application value in predicting cognitive impairment in older adults, and its included clinical predictors can be conveniently obtained from routine assessments.]]> 2026/7/31 0:00:00 1, Li Hui², Sun Yongfu¹, Fan Ruijun³]]> Zhang Jian¹, Li Hui², Sun Yongfu¹, Fan Ruijun³ 58true ［Abstract］　Objective　To analyze the effects of epidermal growth factor-like domain 9(EGFL9) on the EGFR/PI3K/AKT/mTOR signaling pathway and to elucidate the molecular mechanisms by which EGFL9 regulates hepatocellular carcinoma(HCC) cell proliferation, invasion and migration. Methods　Huh-7 cells with EGFL9 knockdown targeting two distinct sites were constructed using lentivirus-mediated small interfering RNA(siRNA) technology. The EGFL9-overexpressing Huh-7 cell and EGFL9-knockdown JHH-7 cell-derived subcutaneously implanted tumors in nude mice constructed by the research team in the previous study were selected for experiments. The EGFL9-overexpressing Huh-7 cells were treated with the EGFR inhibitor GW2974 and the AKT inhibitor MK2206. Cell proliferation was assessed by MTT assay. Cell migratory and invasive capacities were assessed by Transwell assay, and cell apoptosis was detected by flow cytometry. The expression levels of EGFR/PI3K/AKT/mTOR signaling pathway-related proteins were detected by Western blot assay. Results　The proliferative, invasive and migratory capacities of Huh-7 cells with EGFL9 knockdown were significantly inhibited, and their apoptosis rate was increased. The results of Western blot assay showed that EGFL9 knockdown could significantly suppress the expression levels of phosphorylated epidermal growth factor receptor(p-EGFR), phosphorylated phosphatidylinositol 3-kinase(p-PI3K), phosphorylated protein kinase B(p-AKT), and phosphorylated mammalian target of rapamycin(p-mTOR) in both HCC cells and the corresponding xenograft tumors, whereas the expression levels of these phosphorylated proteins increased in the EGFL9-overexpreesing HCC cells(P<0.05). After treatments with EGFR and AKT inhibitors, the phosphorylated protein levels of the pathway proteins related to EGFL9-overexpressing HCC cells were significantly down-regulated(P<0.05), and the cell migratory capacity was weakened. Conclusion　EGFL9 may enhance the proliferative, invasive and migratory capacities of HCC cells via the EGFR/PI3K/AKT/mTOR signaling pathway, and has the potential to serve as a therapeutic target for HCC.]]> 2026/6/30 0:00:00 FENG Jingyang, SHEN Xiaobao, LI Ziye, WANG Bailin, Wu Fan 57true ［Abstract］　Objective　To observe the clinical efficacy and safety of intra-articular injection of concentrated growth factors(CGFs) in treatment of knee osteoarthritis(KOA). Methods　A retrospective analysis was conducted on the clinical data of 253 patients with KOA who were admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region and First Affiliated Hospital of Gannan Medical University from January 2018 to December 2024. Among the 253 patients, 122 patients who received intra-articular injection of CGFs were assigned to the observation group, while the other 131 patients who received intra-articular injection of sodium hyaluronate(SH) were assigned to the control group. The degree of pain［Visual Analogue Scale(VAS) scores］ and the functional condition of knee joints［Western Ontario and McMaster Universities(WOMAC) osteoarthritis index scores and Lysholm Knee Scale(LKS) scores］ were compared between the two groups before the treatment(T₀) and 3 months(T₃) and 6 months(T₆) after the treatment. The recurrence rate of KOA within 6 months after the treatment were compared between the two groups. The occurrence of adverse reactions was observed during the treatment period and the follow-up period. Results　After the treatment, the VAS scores and WOMAC osteoarthritis index scores in both groups showed a significant downward trend(P<0.05), while the LKS scores in both groups showed a significant upward trend(P<0.05). At the time points of T₃ and T₆, the VAS scores and WOMAC osteoarthritis index scores in the observation group were lower than those in the control group, while the LKS scores in the observation group were higher than those in the control group, with statistically significant differences(P<0.05). The recurrence rate of KOA in the observation group was significantly lower than that in the control group within 6 months after the treatment(9.84% vs 51.91%; χ²=51.714, P<0.001). Thirteen cases(10.66%) in the observation group and 18 cases(13.74%) in the control group were reported to have mild pain or swelling at the injection site. All these symptoms relieved themselves. No serious adverse events were observed in either group during the treatment period and the follow-up period. Conclusion　Intra-articular injection of CGFs yields good clinical outcomes in treatment of KOA, with a low recurrence rate and good safety profile, and is worthy of clinical recommendation.]]> 2026/6/30 18:17:12 1,2, LI Xiaozhen³, HUANG Wenwen¹, HUANG Xiao¹, LAI Zhonghong⁴, XU Fangtian⁴, LIN Wenxian⁵, HUANG Jiatao⁶, LI Hongmian⁷]]> LAN Xiaowen^1,2, LI Xiaozhen³, HUANG Wenwen¹, HUANG Xiao¹, LAI Zhonghong⁴, XU Fangtian⁴, LIN Wenxian⁵, HUANG Jiatao⁶, LI Hongmian⁷ 56true ［Abstract］　Objective　To investigate the regulation of the proliferation and migration of hepatocellular carcinoma(HCC) cells by sialic acid-binding immunoglobulin-like lectin 15(Siglec-15) via the EGFR/PI3K/AKT signal pathway. Methods　The expressions of Siglec-15 and epidermal growth factor receptor(EGFR) in HCC tissues and adjacent normal tissues were analyzed by using the Gene Expression Profiling Interactive Analysis 2(GEPIA2) database, and the potential regulatory relationship between Siglec-15 and EGFR was predicted by using the GeneMANIA website. The expressions of Siglec-15 and EGFR in normal hepatocyte L02 and HCC cells HepG2, SMMC-7721 and BEL-7402 were detected by using reverse transcription-quantitative real-time polymerase chain reaction(RT-qPCR) and Western blot. The HepG2 cells were used for subsequent functional assays and were assigned to Siglec-15-NC group, si-Siglec-15 group, EGFR-NC group, si-EGFR group, si-Siglec-15+EGFR-NC group and si-Siglec-15+si-EGFR group, and the transfection efficiency in each interference groups was verified by using RT-qPCR. Western blot assay was used to detect the expressions of Siglec-15 and the EGFR/PI3K/AKT signaling pathway-related proteins in the cells of each group. In addition, methyl thiazolyl tetrazolium(MTT), annexin V-fluorescein isothiocyanate(Annexin V-FITC)/propidium iodide(PI) staining, wound-healing assay and Transwell assay were used to evaluate the individual and combined effects of Siglec-15 and the EGFR/PI3K/AKT signaling pathway on HCC cell proliferation, apoptosis, and migration capacities after transfection. Results　The gene expression levels of Siglec-15 and EGFR in the HCC tissues were significantly higher than those in the adjacent normal tissues(P<0.05), and there was a regulatory relationship between them. The expression levels of Siglec-15 in the SMMC-7721, BEL-7402 and L02 cells were significantly lower than those in the HepG2 cells(P<0.05). The expression levels of EGFR in the SMMC-7721 and L02 cells were significantly lower than those in HepG2 cells(P<0.05). The stable knockdown models of Siglec-15 and EGFR were successfully established in the HepG2 cells. Functional assays demonstrated that the knockdown of either Siglec-15 or EGFR alone significantly inhibited the proliferation and migration capacities of HCC cells and promoted their apoptosis. The concurrent knockdown of Siglec-15 and EGFR synergistically inhibited the proliferation and migration of HCC cells by regulating activation of the PI3K/AKT signaling pathway. Conclusion　Siglec-15 and EGFR are highly expressed in HCC, and there is a regulatory relationship between them. Siglec-15 regulates the proliferation and migration of HCC cells through the EGFR/PI3K/AKT signaling pathway.]]> 2026/6/30 18:17:12 1, GUAN Lulu², LEI Xiaotian³, TIAN Mengmeng³, GUO Cairu², WEN Xuejun⁴, QI Min⁵]]> HOU Shuting¹, GUAN Lulu², LEI Xiaotian³, TIAN Mengmeng³, GUO Cairu², WEN Xuejun⁴, QI Min⁵ 55true ［Abstract］　Objective　To observe the effects of ozonated autohemotherapy(O₃-AHT) on inflammatory response and red blood cell damage in on-pump coronary artery bypass grafting. Methods　A total of 100 patients with coronary atherosclerotic heart disease who were scheduled for coronary artery bypass grafting in the Department of Cardiovascular Surgery of Affiliated Hospital of Guangdong Medical University from October 2024 to December 2025 were recruited. The patients were randomly divided into observation group and control group, with 50 patients in each group. The control group received conventional cardiopulmonary bypass(CPB) cardiac surgery management. The observation group received O₃-AHT within 30 minutes after the start of CPB, with an ozone concentration of 30-40 μg/mL. The levels of inflammatory indicators［interleukin-6(IL-6), interleukin-8(IL-8), tumor necrosis factor-α(TNF-α), C-reactive protein(CRP)］, red blood cell oxidative stress indicators［malondialdehyde(MDA), superoxide dismutase(SOD), glutathione peroxidase(GSH-Px)］, blood gas analysis indicators［partial pressure of arterial oxygen(PaO₂), partial pressure of arterial carbon dioxide(PaCO₂), pH］, routine blood test indicators［hemoglobin(Hb), hematocrit(Hct), red blood cell count(RBC)］ and hemolysis rate were compared between the two groups before anesthesia induction(T₀), 30 minutes after the start of CPB(T₁), at the end of CPB(T₂), 24 hours after the operation(T₃)and 48 hours after the operation(T₄). The morphology of red blood cells was observed at T₀, T₂ and T₄ time points using field emission scanning electron microscopy. The levels of band 3 protein(Band 3) were detected at T₀, T₂ and T₄. The prognosis of the patients within 30 days after the operation was recorded. Results　The levels of PaO₂ at the time points of T₁, T₂, T₃ and T₄ in the observation group were significantly higher than those in the control group(P<0.05), while the levels of PaCO₂ at the time points of T₂ and T₄ in the observation group were significantly lower than those in the control group(P<0.05). There was no statistically significant difference in the level of pH at each time point between the two groups(P>0.05). The levels of IL-6, TNF-α and CRP at the time points of T₂, T₃ and T₄ in the observation group were lower than those in the control group, and the levels of IL-8 at the time points of T₃ and T₄ in the observation group were lower than those in the control group, with statistically significant differences(P<0.05). At the time points of T₁, T₂, T₃ and T₄, the levels of MDA in red blood cells in the observation group were higher than those in the control group; the levels of SOD in red blood cells in the observation group were significantly higher than those in the control group, with statistically significant differences(P<0.05); the levels of GSH-Px in red blood cells in the observation group were lower than those in the control group at T₂, T₃ and T₄(P<0.05). At the time points of T₁, T₂, T₃ and T₄, the hemolysis rates in the observation group were significantly lower than those in the control group(P<0.05); the RBC levels in the observation group were significantly higher than those in the control group(P<0.05). At the time points of T₂, T₃ and T₄, the levels of Hb and Hct in the observation group were significantly higher than those in the control group(P<0.05). At the time points of T₂ and T₄, the circularity of red blood cells in the observation group was better than that in the control group; the diameter of red blood cells in the observation group was greater than that in the control group; the expression levels of Band 3 in the observation group were higher than those in the control group, with statistically significant differences(P<0.05). The length of intensive care unit(ICU) stay and the length of postoperative hospital stay in the observation group were significantly shorter than those in the control group(P<0.05). There were no statistically significant differences in the incidence rates of low cardiac output syndrome, infection, and major adverse cardiovascular events(MACE) between the two groups(P>0.05). Conclusion　In concentrations of ozone ranging from 30 μg/mL to 40 μg/mL, O₃-AHT can effectively inhibit the inflammatory response during CPB in coronary artery bypass grafting, alleviate red blood cell oxidative stress and membrane damage, improve postoperative lung function, and shorten the length of ICU stay and the length of postoperative hospital stay.]]> 2026/6/30 18:17:12 1,2, LI Daheng³, LI Mingliang⁴, ZHOU Chengbin², TANG Jing³]]> LIN Zhonglin^1,2, LI Daheng³, LI Mingliang⁴, ZHOU Chengbin², TANG Jing³ 54true ［Abstract］　Objective　To compare the diagnostic efficacy of endocytoscopy(EC) and magnifying endoscopy with narrow-band imaging(ME-NBI) for early esophageal cancer. Methods　A total of 184 patients who underwent white-light endoscopy(WLE) in the Endoscopy Center of the Main Campus of Cangzhou People′s Hospital from June 2022 to April 2025 and were found to have suspected esophageal mucosal lesions were recruited. These patients were divided into EC group(95 patients) and ME-NBI group(89 patients) according to the different endoscopic examinations they received. Two patients in the ME-NBI group voluntarily withdrew from the study due to the aggravation of acute mucosal inflammation before the examination. One patient in each of the two groups was excluded due to the unqualified pathological biopsy specimens. Eventually, 94 patients in the EC group and 86 patients in the ME-NBI group were included for analysis. The results of the EC group were interpreted according to the Endocytoscopic Atypia(ECA) classification. The results of the ME-NBI group were interpreted according to China guideline for the screening, early detection and early treatment of esophageal cancer(2022, Beijing) and Japanese Esophageal Society′s Intrapapillary Capillary Loop Classification(IPCL) criteria. Taking the results of pathological diagnosis as the gold standard, Kappa consistency analysis was applied to explore the consistency between the results of EC and ME-NBI examinations and the results of pathological examination. The diagnostic agreement rate, positive percent agreement(PPA) and negative percent agreement(NPA) were compared between the two groups. The agreement rates of judging stratification of lesion infiltration depth, classification of endoscopic morphology and the maximum diameter of lesions were compared between the two groups. Results　In terms of judging cancer and non-cancer, the results of EC examination and ME-NBI examination showed good consistency with the results of pathological examination(Kappa=0.575, P<0.001; Kappa=0.346, P<0.001). The diagnostic agreement rate, PPA and NPA in the EC group were higher than those in the ME-NBI group. In terms of judging stratification of lesion infiltration depth, the results of EC examination and ME-NBI examination showed good consistency with the results of pathological examination(Kappa=0.871, P<0.001; Kappa=0.780, P<0.001). There were no significant differences in the agreement rates of judging different infiltration depths between the EC group and the ME-NBI group. There were no differences between the two groups in the agreement rates of diagnosing early-stage digestive tract tumors with Paris Classification type Ⅰ, and the diagnostic agreement rates of type Ⅱb and type Ⅱc in the EC group were higher than those in the ME-NBI group. The judging agreement rate of the longest diameter of the lesions ≤1 cm in the EC group was higher than that in the ME-NBI group. There was no statistically significant difference in the judging agreement rate of the longest diameter of the lesions >1 cm between the two groups. Conclusion　The diagnostic accuracy of the results of EC examination is better than that of ME-NBI examination for early-stage esophageal cancer, particularly in judging micro-type lesions and flat-type lesions. ME-NBI has certain advantages in assessment of deep infiltrative lesions. The combined application of EC and ME-NBI is helpful to improve the diagnostic accuracy of early-stage esophageal cancer.]]> 2026/6/30 18:17:12 1, LI Dongmei¹, WANG Weiyi¹, CAI Chang²]]> ZHOU Yang¹, LI Dongmei¹, WANG Weiyi¹, CAI Chang² 53true ［Abstract］　Objective　To explore the impact of domestic pet exposure on allergic diseases in children with animal dander allergy, and to further understand the relationship between pet exposure and allergic diseases in children and to provide evidence for clinical diagnosis and treatment. Methods　A retrospective analysis was conducted on the clinical data of 399 children with bronchial asthma, allergic rhinitis, atopic dermatitis, acute urticaria or allergic conjunctivitis who visited doctors in the pediatric outpatient clinic of Beijing Tsinghua Changgung Hospital, Tsinghua University from December 2017 to December 2024, and were positive for specific immunoglobulin E(sIgE) to animal dander in serum inhalant allergen testing. According to whether there was a history of exposure to domestic pets, the children were divided into domestic pet exposure group(162 children) and non-domestic pet exposure group(237 children). The clinical data, including gender, age, type of allergic diseases, a family history of allergic diseases in first-degree relatives, total serum IgE level, and sIgE to animal dander in serum inhalant allergen testing, were compared between the children in the two groups. Multivariate logistic regression analysis was used to identify the influencing factors for bronchial asthma. Results　In the 399 children, 285 children(71.43%) had allergic rhinitis; 171 children(42.86%) had bronchial asthma; 66 children(16.54%) had atopic dermatitis; 45 children(11.28%) had acute urticaria, and 41 children(10.28%) had allergic conjunctivitis. In the 399 children, 40.60% of them had domestic pets. The time from initial pet contact to diagnosis of an allergic disease in the pet exposure group was 2.70(2.00, 4.00)years. Compared with the non-domestic pet exposure group, the domestic pet exposure group had a lower proportion of first-degree relatives with a history of allergic diseases, lower serum total IgE level, a lower prevalence of bronchial asthma, and a lower detection rate of mixed animal dander allergies combined with two or more other inhalant allergens, while the domestic pet exposure group was older, and had a later age at diagnosis of allergic disease, and a higher detection rate of single animal dander allergy, with statistically significant differences(P<0.05). The results of multivariate logistic regression analysis showed that exposure to domestic pets was a protective factor for inhibiting the occurrence of bronchial asthma(P<0.05), while a family history of allergic diseases in first-degree relatives was a risk factor for bronchial asthma(P<0.05). Conclusion　Exposure to domestic pets during the years before the diagnosis of allergic diseases is significantly associated with a reduced risk of bronchial asthma in children with animal dander allergy, a delayed age at diagnosis of allergic diseases, lower serum total IgE level, and a decrease in polysensitization. Additionally, a family history of allergic diseases in first-degree relatives is significantly associated with bronchial asthma in children.]]> 2026/6/30 18:17:12 1, YIN Xiaomei¹, YUAN Ke¹, ZHAO Zhipeng², CHAO Shuang¹]]> WANG Yuejiao¹, YIN Xiaomei¹, YUAN Ke¹, ZHAO Zhipeng², CHAO Shuang¹ 52true ［Abstract］　Objective　To investigate the effect of the timing of dexamethasone acetate(DXM) intervention in controlled ovarian stimulation(COS) cycles on assisted pregnancy outcomes. Methods　A retrospective analysis was conducted on the clinical data of 450 patients(a total of 450 cycles) undergoing initial in vitro fertilization and embryo transfer(IVF-ET) by using the follicular phase long-acting long protocol in the Centre for Reproductive Medicine of Changde Hospital, Xiangya School of Medicine, Central South University(the First People′s Hospital of Changde City) from January 2022 to June 2024. The patients were divided into non-DXM group(group A, 117 cases) and DXM intervention group(group B, 333 cases). Group B was further subdivided into three subgroups according to the timing of DXM intervention: early-stage DXM group［group B1, 205 cases, DXM was administered at the initiation of using gonadotropin(Gn)］, mid-stage DXM group(group B2, 54 cases, DXM was administered at 6 days after using Gn or when the diameter of dominant follicles was 10-14 mm), and late-stage DXM group［group B3, 74 cases, DXM was administered when the diameter of dominant follicles was >16 mm or DXM was administered when serum progesterone(P) level was ≥1 ng/mL］. The general data, COS protocols, hormonal parameters, core embryonic laboratory indicators, and assisted pregnancy outcomes were compared among the four groups. Results　There were no significant differences among the four groups in age, infertility duration, body mass index(BMI), anti-Müllerian hormone(AMH), and baseline levels of follicle-stimulating hormone(FSH), luteinizing hormone(LH), estradiol(E₂) and P(P>0.05). There was no statistically significant difference in Gn starting dose(GSD) among the four groups(P>0.05). The total Gn dose in group B1 was significantly lower than that in group A, and the duration of using Gn in group B1 was significantly shorter than that in group A, with statistically significant differences(P<0.05). On the trigger day, the E₂ levels in group B1, group B2 and group B3 were higher than those in group A, and the E₂ level in group B3 was significantly higher than that in group B1, with statistically significant differences(P<0.05). The P levels in group B3 and group B2 were significantly higher than those in group A and group B1(P<0.05). Group B3 had a higher number of oocytes retrieved than group A, and a higher rate of metaphase Ⅱ(MⅡ) oocytes than group B1, and a higher number of transferable embryos than group B1, with statistically significant differences(P<0.05). The blastocyst formation rate in group B1 was lower than that in group A, with statistically significant difference(P<0.05). There were no significant differences among the four groups in the two-pronuclear zygote(2PN) fertilization rate, 2PN cleavage rate, number of top-quality embryos and top-quality embryo rate(P>0.05). The rate of fresh embryo transfer was higher in group A and group B1 than in group B2, and the cycle cancellation rate due to elevated P was lower in group A than in group B1, group B2, and group B3, with statistically significant differences(P<0.05). Group B1 had the highest clinical pregnancy rate(69.29%) and live birth rate(56.67%), but the differences among the four groups were not statistically significant(P>0.05). Conclusion　In COS cycles using the follicular phase long-acting long protocol, adding DXM at early stage can effectively control P levels, reduce Gn dosage and days of stimulation, and increase the rate of fresh embryo transfer, thereby improving the assisted pregnancy outcomes.]]> 2026/6/30 18:17:12 1, TANG Bin¹, CHENG Chong², QIN Yaqian¹, TANG Jia¹, GUO Yabin¹]]> ZHANG Ling¹, TANG Bin¹, CHENG Chong², QIN Yaqian¹, TANG Jia¹, GUO Yabin¹ 51true ［Abstract］　Objective　To analyze the serum levels of microRNA-181a-5p(miR-181a-5p) and microRNA-132-5p(miR-132-5p) in diabetic kidney disease(DKD) patients and their correlations with kidney injury and prognosis. Methods　A total of 121 patients with DKD who were admitted to Lianyungang Affiliated Hospital of Nanjing University of Chinese Medicine from April 2021 to April 2024 were recruited as the DKD group, and 119 patients with diabetes only were recruited as the diabetes-only group, and 116 healthy individuals undergoing physical examination were recruited as the control group. Treatment interventions were performed according to the actual situation of the DKD patients, and their blood glucose, glycated hemoglobin A₁c(HbA₁c), blood pressure and blood lipid were strictly controlled. The DKD patients were followed up for 1 year. Poor prognosis was considered if the patients′ serum creatinine(Scr) level doubled or the glomerular filtration rate decreased by 50% during the follow-up period. The levels of serum miR-181a-5p and miR-132-5p of the research subjects were detected and compared. The correlations of serum miR-181a-5p and miR-132-5p levels with glomerular grades, interstitial inflammation scores, interstitial fibrosis and tubular atrophy(IFTA) scores in the DKD patients were analyzed. Multivariate logistic regression was used to analyze the factors influencing the poor prognosis of the DKD patients. The efficacy of serum miR-181a-5p and miR-132-5p levels in predicting poor prognosis of the DKD patients was analyzed by using receiver operating characteristic(ROC) curve. Results　The levels of serum miR-181a-5p and miR-132-5p in the DKD group were lower than those in the control group and the diabetes-only group. The levels of serum miR-181a-5p and miR-132-5p in the diabetes-only group were lower than those in the control group, and the differences were statistically significant(P<0.05). The levels of serum miR-181a-5p and miR-132-5p were negatively correlated with glomerular grades, interstitial inflammation scores and IFTA scores(P<0.05). Ninety-six patients with DKD had a favorable prognosis, while 25 patients with DKD had an unfavorable prognosis. The results of multivariate logistic regression analysis showed that elevated serum miR-181a-5p［OR(95%CI)=0.816(0.710-0.938)］ and elevated miR-132-5p［OR(95%CI)=0.753(0.632-0.897)］ were independent protective factors for inhibiting poor prognosis in the DKD patients(P<0.05). The results of ROC curve analysis showed that the levels of serum miR-181a-5p and miR-132-5p could effectively predict the poor prognosis of the DKD patients(P<0.05), and the predictive efficacy of the combination of the two indicators was better than that of a single indicator(Z=2.278, P=0.036; Z=2.612, P=0.025), with a sensitivity of 91.74% and a specificity of 99.67%. Conclusion　The levels of serum miR-181a-5p and miR-132-5p in DKD patients are significantly decreased, which is closely related to the degree of kidney injury and prognosis. The combined detection of the two indicators can better predict the prognosis of the DKD patients.]]> 2026/6/30 18:17:13 1, ZHAO Xiaohui², ZHANG Huaming¹, HOU Sinan²]]> WANG Li¹, ZHAO Xiaohui², ZHANG Huaming¹, HOU Sinan² 50true ［Abstract］　Objective　To explore the clinical efficacy of Guben Qushi Huayu formula(GQHF) combined with febuxostat in treatment of gouty nephropathy(GN) and its influence on levels of oxidative stress indicators. Methods　A total of 106 GN patients admitted to Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine from January 2022 to January 2025 were recruited and divided into observation group and control group by random number table method, with 53 patients in each group. The control group was treated with febuxostat, while the observation group was treated with GQHF combined with febuxostat. The patients in both groups were treated for 3 months. The scores of main traditional Chinese medicine(TCM) syndromes, renal function indicators, urinary protein indicators and levels of oxidative stress indicators were compared between the two groups before and after the treatment. The clinical efficacy and the occurrence of adverse reactions were compared between the two groups. Results　After the treatment, the TCM syndrome scores of joint pain, joint swelling, skin numbness, and difficulty in flexion and extension in the observation group were lower than those in the control group, and the levels of serum creatinine(Scr), blood urea nitrogen(BUN), uric acid(UA), malondialdehyde(MDA), urinary β₂-microglobulin(Uβ₂-MG), urinary albumin-to-creatinine ratio(UACR) and 24-hour urinary total protein(24hUTP) in the observation group were lower than those in the control group, and the levels of estimated glomerular filtration rate(eGFR), superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) in the observation group were higher than those in the control group, with statistically significant differences between the two groups(P<0.05). The total clinical effective rate of the observation group was significantly higher than that of the control group(94.34% vs 81.13%; χ²=4.296, P=0.038 ). There was no statistically significant difference in the total incidence of adverse reactions between the two groups(15.09% vs 11.32%; χ²=0.329, P=0.566). Conclusion　The combination of GQHF and febuxostat is safe in treatment of GN and can effectively improve the patients′ clinical symptoms and renal function. The combined treatment may exert the effects by reducing the levels of oxidative stress indicators.]]> 2026/6/30 18:17:13 XIN Jiechen, WANG Xiaoyan, ZHANG Feng, ZHAO Longshu 49true ［Abstract］　Objective　To analyze the expressions of immediate early response 3(IER3) in colorectal cancer tissues and circulating tumor cells(CTCs) and their clinical significance, and to clarify the application potential of IER3 as a tumor biomarker for colorectal cancer, and to provide reference for the prognosis assessment of colorectal cancer patients and the development of therapeutic drugs. Methods　A total of 63 patients with colorectal cancer who received radical surgical treatment in the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2017 to September 2024 were recruited as the research subjects. The patients′ cancer tissues and adjacent paracancerous tissues were collected. Immunohistochemistry and real-time fluorescence quantitative polymerase chain reaction(RT-qPCR) were used to detect the protein and mRNA expressions of IER3, respectively. The patients′ peripheral blood samples were collected 1 day before the operation and on the 7th day after the operation. The cell count and classification of CTCs in the peripheral blood were detected by using CanPatrol® filtration membrane technology, and the expression of IER3 mRNA in CTCs was detected by using in situ hybridization technology. Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling(TUNEL) was used to evaluate tumor cell apoptosis. The follow-up was conducted through phone calls or outpatient reexaminations. The last follow-up date was January 15, 2025. The correlations of IER3 expression with the degree of tumor cell apoptosis, disease recurrence and metastatic risk, and prognosis of the patients were analyzed. Results　The positive expression rate of IER3 protein in cancer tissues was significantly higher than that in adjacent paracancerous tissues(90.47% vs 15.87%, χ²=10.833, P<0.001). The relative expression level of IER3 mRNA in cancer tissues was significantly higher than that in adjacent paracancerous tissues［(1.51±0.51) vs (1.13±0.63), t=3.200, P=0.002］. The expression level of IER3 mRNA in colorectal cancer tissues was significantly correlated with the degree of tumor cell apoptosis(P<0.05). The cell count of IER3 mRNA-positive CTCs in each subtype was correlated with the depth of tumor invasion(P<0.05), whereas the cell count of IER3 mRNA-positive mesenchymal CTCs was correlated with vascular invasion, lymph node metastasis and clinical stages(P<0.05). No patients in this study were lost to follow-up, among whom 12 patients died of colorectal cancer. The patients with the postoperative cell count of IER3 mRNA-positive epithelial CTCs>1 exhibited significantly lower postoperative cumulative survival rate than those with the postoperative cell count of IER3 mRNA-positive epithelial CTCs<1(P<0.05). Conclusion　IER3 is highly expressed in colorectal cancer tissues and correlated with the degree of tumor cell apoptosis. The cell count of IER3 mRNA-positive CTCs in various subtypes can serve as indicators for evaluating tumor invasiveness and predicting prognosis, and IER3 is expected to be a potential biomarker for risk stratification of recurrence and metastasis, precise prognostic assessment and individualized treatment of colorectal cancer.]]> 2026/6/30 0:00:00 1, LI Songlin², ZHOU Rizhong³, QI Dengping⁴, ZHONG Xiaogang⁴]]> WEI Chunying¹, LI Songlin², ZHOU Rizhong³, QI Dengping⁴, ZHONG Xiaogang⁴ 48true ［Abstract］　Objective　To observe the clinical effect of modified Jisheng Shenqi Decoction combined with Astragalus injection at acupoints in treatment of viral hepatitis B-related cirrhosis. Methods　A total of 94 patients with viral hepatitis B-related cirrhosis who were admitted to Liuzhou Traditional Chinese Medical Hospital from January 2024 to January 2025 were recruited as the research subjects. The patients were divided into control group and observation group by using random number table method, with 47 patients in each group. The control group received conventional medical treatment, while the observation group was given the same treatment as the control group plus modified Jisheng Shenqi Decoction combined with Astragalus injection at acupoints. Both groups were treated continuously for 12 weeks. The Chronic Liver Disease Questionnaire(CLDQ) scores, Child-Turcotte-Pugh(CTP) scores, liver stiffness measurement(LSM) values, and biochemical indicators were compared between the two groups before and after the treatment. The occurrence of adverse reactions during the treatment period in both groups were recorded. Results　Among the 94 patients, 5 patients withdrew from the study during the treatment period, including 2 patients in the control group and 3 patients in the observation group. Compared with those before the treatment, the CLDQ scores were significantly increased(P<0.05), and the CTP scores and the levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST) and total bilirubin(TBIL) were significantly decreased(P<0.05) and the prothrombin time(PT) was significantly shortened after the treatment in the two groups(P<0.05). Compared with those before the treatment, the LSM values and the levels of hyaluronic acid(HA), laminin(LN), type Ⅲ procollagen(PC-Ⅲ) and type Ⅳ collagen(Ⅳ-C) were significantly decreased after the treatment in the observation group(P<0.05), while the albumin(ALB) levels were significantly increased after the treatment in the control group(P<0.05). After the treatment, the CLDQ scores in the observation group were significantly higher than those in the control group(P<0.05), while the LSM values and the levels of ALT, AST, TBIL, ALB, HA, LN, PC-Ⅲ and Ⅳ-C in the observation group were significantly lower than those in the control group(P<0.05). During the treatment period, no serious adverse reactions occurred in either group. Conclusion　The combination of modified Jisheng Shenqi Decoction with Astragalus injection at acupoints is significantly effective in treating viral hepatitis B-related cirrhosis. The combined treatment can effectively improve the patients′ liver function, reduce the degree of liver fibrosis, and enhance their quality of life.]]> 2026/5/29 19:40:21 1, LIN Jun², HAN Lu¹, YU Junying², WANG Wenshuai², WEI Xuelian², WU Chan²]]> LI Zepeng¹, LIN Jun², HAN Lu¹, YU Junying², WANG Wenshuai², WEI Xuelian², WU Chan² 47true ［Abstract］　Objective　To investigate the mechanisms of chromobox protein homolog 2(CBX2) in colorectal cancer(CRC) progression and 5-fluorouracil(5-FU) resistance. Methods　The relationship between the expression of CBX2 in CRC and clinicopathological characteristics, as well as its impact on the patients′prognosis was analyzed by using databases and clinical data. The expression levels of CBX2 in CRC samples were detected by using immunohistochemical staining, Western blot and real-time fluorescent quantitative polymerase chain reaction( RT-qPCR). The CBX2 down-regulated and overexpressed cell models were constructed, and their effects on CRC cell proliferation, migration, invasion and sensitivity to 5-FU were evaluated by using CCK-8 assay, clone formation assay and Transwell assay. Bioinformatics analysis and cell experiments were conducted to verify the regulatory effects of CBX2 in CRC on the ephrin type-A receptor 3(EphA3)/PI3K/AKT/mTOR signaling pathway, as well as its influence on the malignant biological behavior of CRC cells and sensitivity to 5-FU. Animal experiments were conducted to verify the effect of CBX2 on the in vivo tumorigenic ability of CRC cells. Results　CBX2 was significantly highly expressed in CRC tissues, and its expression level was significantly correlated with tumor clinical stage, tumor differentiation degree and lymph node metastasis. Positive expression of CBX2 was a risk factor for the CRC patients′ poor prognosis. Down-regulation of CBX2 could inhibit the proliferation, migration and invasion of CRC cells, and enhanced their sensitivity to 5-FU, while the effects of overexpressing CBX2 were the opposite. CBX2 could up-regulate EphA3 and bind it directly. Down-regulation of CBX2 reduced the expression of EphA3 and inhibited the PI3K/AKT/mTOR signaling pathway, thereby suppressing the proliferation, migration, invasion of CRC cells and their sensitivity to 5-FU. CBX2 promoted the tumorigenesis of CRC cells in nude mice. Conclusion　CBX2 is highly expressed in CRC and is associated with poor prognosis. The molecular mechanisms may be related to that CBX2 promotes the proliferation, migration and invasion of CRC cells and their resistance to 5-FU by binding to the EphA3 protein and activating the PI3K/AKT/mTOR signaling pathway.]]> 2026/5/29 19:40:21 1, MO Chongde², HOU Qiyan¹, ZHAO Jiehua¹, TANG Yi¹, ZHANG Qiuhuan¹]]> YU Zehao¹, MO Chongde², HOU Qiyan¹, ZHAO Jiehua¹, TANG Yi¹, ZHANG Qiuhuan¹ 46true ［Abstract］　Objective　To explore the application value of vaginal ultrasound combined with serum Ki-67 and alpha-fetoprotein(AFP) in differentiating immature ovarian teratoma from mature ovarian teratoma. Methods　A total of 135 patients with ovarian teratoma who were admitted to Ankang Central Hospital from January 2023 to July 2024 were recruited and divided into mature ovarian teratoma group(n=108) and immature ovarian teratoma group(n=27) according to the postoperative pathological results. Multivariate logistic regression was used to analyze the factors that were helpful in differentiating immature ovarian teratoma from mature ovarian teratoma. The receiver operating characteristic(ROC) curve was used to analyze the efficacy of serum Ki-67 and AFP levels in differentiating immature ovarian teratoma from mature ovarian teratoma. Kappa consistency analysis was used to explore the consistency between vaginal ultrasound examination results and pathological examination results. The efficacy of different examination methods in differentiating immature ovarian teratoma from mature ovarian teratoma was compared. Results　The results of multivariate logistic regression analysis showed that spiral artery pulsatility index, spiral artery resistance index, uterine artery pulsatility index, uterine artery resistance index, endometrial thickness, blood flow index, vascular index, vascularization flow index, AFP and Ki-67 were the factors that were helpful in differentiating immature ovarian teratoma from mature ovarian teratoma(P<0.05). The results of ROC curve analysis showed that the levels of serum Ki-67［AUC(95%CI)=0.767(0.354-0.946)］ and AFP［AUC(95%CI)=0.714(0.321-0.934)］ could well differentiate immature ovarian teratoma from mature ovarian teratoma(P<0.05). The results of Kappa consistency analysis indicated a good consistency between the diagnostic results of vaginal ultrasound examination and pathological examination(Kappa=0.606, P<0.001). The accuracy rate of vaginal ultrasound examination combined with serum Ki-67 and AFP in differentiating immature ovarian teratoma from mature ovarian teratoma was 93.33%, which was higher than that of a single examination indicator and the combined examination of serum Ki-67 and AFP. Conclusion　Vaginal ultrasound combined with serum Ki-67 and AFP can effectively differentiate immature ovarian teratoma from mature ovarian teratoma, which is helpful for the early identification of high-risk patients and guiding the formulation of treatment regimens in clinical practice. It is worthy of recommendation.]]> 2026/5/29 19:40:21 DING Kun, YUAN Shenglan, TAN Hongjun 45true ［Abstract］　Objective　To observe the clinical efficacy of roflumilast tablets combined with fluticasone furoate, umeclidinium bromide and vilanterol trifenatate powder for inhalation in treatment of acute exacerbation of chronic obstructive pulmonary disease(AECOPD) and its effects on serum monocyte chemoattractant protein-1(MCP-1) and interleukin-8(IL-8) levels. Methods　A total of 200 patients with AECOPD who were admitted to Shangluo Central Hospital from February 2022 to February 2024 were recruited and randomly divided into observation group(receiving the treatment of roflumilast tablets combined with fluticasone furoate, umeclidinium bromide and vilanterol trifenatate powder for inhalation) and control group(receiving fluticasone furoate, umeclidinium bromide and vilanterol trifenatate powder for inhalation) by using random number table method, with 100 patients in each group. Both groups were treated continuously for 2 months. The levels of blood gas analysis indicators, inflammatory factors and pulmonary function indicators were compared between the two groups before and after the treatment. The clinical efficacy and the incidence of adverse reactions were compared between the two groups. Results　Compared with those before the treatment, the levels of partial pressure of arterial oxygen(PaO₂) and oxygen saturation(SaO₂) in the two groups significantly increased after the treatment(P<0.05), while the levels of partial pressure of arterial carbon dioxide(PaCO₂) in both groups significantly decreased after the treatment(P<0.05). Compared with those in the control group, the levels of PaO₂ and SaO₂ were higher, while the levels of PaCO₂ were lower in the observation group after the treatment, and the differences were statistically significant(P<0.05). The levels of MCP-1, C-reactive protein(CRP) and IL-8 in the two groups after the treatment were significantly lower than those before the treatment(P<0.05), and the levels of forced expiratory volume in 1 second(FEV₁), forced vital capacity(FVC), and peak expiratory flow(PEF) in the two groups after the treatment were significantly higher than those before the treatment(P<0.05). Moreover, the levels of MCP-1, CRP and IL-8 in the observation group were lower than those in the control group after the treatment, while the levels of FEV₁, FVC and PEF in the observation group were higher than those in the control group after the treatment, and the differences were statistically significant(P<0.05). The total clinical effective rate of the observation group was significantly higher than that of the control group(97.00% vs 88.00%; χ²=5.838, P=0.016). There was no statistically significant difference in the total incidence of adverse reactions between the two groups(12.00% vs 13.00%; χ²=0.046, P=0.831). Conclusion　Roflumilast tablets combined with fluticasone furoate, umeclidinium bromide and vilanterol trifenatate powder for inhalation are significantly effective and safe in treatment of AECOPD. The combined treatment can effectively improve the blood gas condition and lung function of the patients and reduce their inflammatory factor levels, and is worthy of clinical recommendation.]]> 2026/5/29 19:40:21 1, LI Kai¹, ZHANG Sanhong²]]> WANG Qinqin¹, LI Kai¹, ZHANG Sanhong² 44true ［Abstract］　Objective　To study the role and mechanism of melatonin(MT) in trophoblast cells during preeclampsia(PE) based on the NOD-like receptor protein 3(NLRP3) signaling pathway. Methods　The model of trophoblast cells during PE was constructed by treating HTR8/SVneo cells for 48 hours under the condition of hypoxia. Cell proliferation levels were detected by using the CCK8 assay. The control group(HTR8/SVneo cells), PE group, PE+100 μmol/L MT group, PE+500 μmol/L MT group and PE+1 mmol/L MT group were set up. The cell mortality rate in each group was detected by using flow cytometry, and the invasive ability of cells in each group was detected by using Transwell assay. The expression levels of NLRP3 signaling pathway-related factor NLRP3, apoptosis-associated speck-like protein containing a CARD(ASC), caspase-1 and interleukin-1β(IL-1β) in the cells of each group were detected by using quantitative real-time polymerase chain reaction(qRT-PCR) and Western blot assay. Results　MT at concentrations ≤1 mmol/L had no significant effect on the proliferation of HTR8/SVneo cells. The cell mortality rate in the PE group was significantly higher than that in the control group(P<0.05). Compared with that in the PE group, the cell mortality rate in the PE+100 μmol/L MT group, the PE+500 μmol/L MT group and the PE+1 mmol/L MT group was significantly decreased(P<0.05). The cell invasion rate in the PE group was significantly lower than that in the control group(P<0.05). Compared with that in the PE group, the cell invasion rate in the PE+500 μmol/L MT group and the PE+1 mmol/L MT group was significantly increased(P<0.05), and the cell invasion rate showed an upward trend with the increase of the intervention concentration of MT. Compared with those of the control group, the mRNA and protein expression levels of NLRP3, ASC, caspase-1 and IL-1β in the cells of the PE group were significantly increased(P<0.05), while the intervention of MT could reverse these changes. Conclusion　MT can reduce the mortality rate of trophoblast cells during PE and enhance their invasive ability. The mechanisms may be related to the inhibition of the NLRP3 signaling pathway.]]> 2026/5/29 19:40:21 1, LIANG Xuxia², HU Qiuwen², QIN Zhiping³, MA Yanhua², HU Qiongyan², LU Jun², LI Hui¹, LU Yebin²]]> PAN Ziyang¹, LIANG Xuxia², HU Qiuwen², QIN Zhiping³, MA Yanhua², HU Qiongyan², LU Jun², LI Hui¹, LU Yebin² 43true ［Abstract］　Objective　To analyze the value of spectral computed tomography(CT) single-energy imaging parameters combined with microRNA-146a(miR-146a) in predicting lower extremity deep vein thrombosis(LEDVT) in patients after vascular surgery. Methods　A total of 258 patients who underwent vascular surgery in the Affiliated Suzhou Hospital of Nanjing University Medical School(Suzhou Science & Technology Town Hospital) from January 2022 to November 2024 were recruited as the research subjects, including 70 patients undergoing aortic endovascular repair, 96 patients undergoing carotid endarterectomy, 48 patients undergoing acute arterial embolectomy, and 44 patients undergoing open abdominal aortic aneurysm repair. These patients were divided into LEDVT group(50 patients) and non-LEDVT group(208 patients) according to whether they developed LEDVT within 3 months after vascular surgery. Both groups underwent spectral CT single-energy imaging examination and detection of serum miR-146a level before vascular surgery. The independent variables were screened using LASSO regression, and multivariate logistic regression was used to analyze the influencing factors of developing LEDVT in patients after vascular surgery. The efficacy of spectral CT single-energy imaging parameters combined with miR-146a in predicting the development of LEDVT in patients after vascular surgery was analyzed by using receiver operating characteristic(ROC) curve. Results　The levels of fibrinogen(Fib) and D-dimer(D-D) in the LEDVT group were significantly higher than those in the non-LEDVT group(P<0.05). The thrombin time(TT) in the LEDVT group was significantly longer than that in the non-LEDVT group(P<0.05). The CT values of inferior vena cava, femoral vein and popliteal vein under 40-70 keV energy level in the LEDVT group were higher than those in the non-LEDVT group, and the miR-146a level in the LEDVT group was lower than that in the non-LEDVT group, with statistically significant differences between the two groups(P<0.05). Four independent variables were screened out by using LASSO regression analysis, which were the CT values of inferior vena cava, femoral vein and popliteal vein under 40-70 keV energy level and miR-146a. The results of multivariate logistic regression analysis showed that the elevated CT values of inferior vena cava, femoral vein and popliteal vein under 40-70 keV energy level were risk factors of developing LEDVT in patients after vascular surgery(P<0.05), and lower level of miR-146a was a protective factor of inhibiting LEDVT in patients after vascular surgery(P<0.05). The results of ROC curve analysis showed that the CT values of inferior vena cava, femoral vein and popliteal vein under 40-70 keV energy level and miR-146a could effectively predict the development of LEDVT in patients after vascular surgery(P<0.05), and the predictive efficacy of the combined detection of the 4 indicators［AUC(95%CI)=0.897(0.853-0.931)］ was better than that of a single indicator(P<0.05). Conclusion　Spectral CT single-energy imaging parameters and miR-146a are related to the development of LEDVT in patients after vascular surgery. The CT values of inferior vena cava, femoral vein and popliteal vein under 40-70 keV energy level combined with miR-146a level have good predictive value for postoperative LEDVT.]]> 2026/5/29 19:40:21 1, WU Qiong¹, TIAN Jinjie¹, SUN Yapeng², CHENG Bo¹]]> DING Xiang¹, WU Qiong¹, TIAN Jinjie¹, SUN Yapeng², CHENG Bo¹ 42true ［Abstract］　Objective　To compare multilocus microsatellite typing(MLMT) characteristics of 142 Cryptococcus neoformans var. grubii strains isolated from China, Japan, and Brazil. Methods　A total of 142 isolated strains of Cryptococcus neoformans var. grubii were selected, including 40 strains from China, 13 strains from Japan, and 89 strains from Brazil. In the 142 isolated strains, 73 strains(30 strains from China, 7 strains from Japan and 36 strains from Brazil) were isolated from clinical patient specimens, and the other 69 strains(10 strains from China, 6 strains from Japan, 53 strains from Brazil) were isolated from environmental samples(pigeon droppings). All the strains were provided by Medical Mycology Research Center, Chiba University, Japan. Strain DNAs were extracted, and the microsatellite loci CNG1(TA), CNG2(GA), and CNG3(CAT) were amplified and sequenced. Genotypes were determined by counting motif repeats using Chromas software, and genotype diversity was assessed based on the Simpson′s index(D). Results　In the 142 strains, 17 MLMT genotypes were identified. Six MLMT genotypes were identified in the strains from China, 2 MLMT genotypes in the strains from Japan, and 13 MLMT genotypes in the strains from Brazil. The MLMT-17 genotype was the most prevalent(38.03%, 54/142) and was identified in both clinical and environmental specimens in the three countries. In addition, the MLMT-2 genotype and the MLMT-34 genotype had been identified in the three countries. The Simpson′s index(D) of the strains was 0.32, 0.15, and 0.82 for China, Japan and Brazil, respectively, with the strains in Brazil exhibiting higher genotype diversity than those in China(χ²=76.521, P<0.001) and Japan(Fisher′s exact test, P<0.001). However, the comparison of the genetic diversity of the strains between China and Japan showed no statistically significant difference(Fisher′s exact test, P=0.287). Stratified comparison by sample origin revealed that the genotypic diversity in environmental isolates was significantly higher than that in clinical isolates［Simpson′s index(D): 0.68 vs 0.76; χ²=54.810, P<0.001］. Conclusion　The genotype composition of Cryptococcus neoformans var. grubii strains varies by region. In China and Japan, the MLMT-17 genotype is predominant, while in Brazil, the MLMT-36 genotype is predominant, and the genotype diversity in Brazil is the highest.]]> 2026/5/29 19:40:21 1, ZHU Jian², SHI Rongxing¹, HE Tingting¹, ZHANG Wei³]]> LU Qin¹, ZHU Jian², SHI Rongxing¹, HE Tingting¹, ZHANG Wei³ 41true ［Abstract］　Objective　To analyze the clinical characteristics of Mycoplasma pneumoniae(MP) infection-associated juvenile arthritis and to enhance clinicians′ understanding of the diagnosis and treatment of the disease. Methods　A retrospective analysis was conducted on the clinical data of 30 pediatric patients with MP infection-associated juvenile arthritis who were admitted to Department of Pediatrics, Jiading Branch, Renji Hospital, Shanghai Jiao Tong University School of Medicine from January 1, 2024 to June 30, 2025. The clinical characteristics, imaging examination results, treatment and prognosis of the patients were summarized. Results　Among the 30 pediatric patients, 11 patients were male and 19 patients were female. The onset age ranged from 1 year and 6 months to 13 years, with an average onset age of (8.2±2.4)years, and was predominantly in school ages(6～<10 years). The initial clinical symptoms of all the patients were manifested as joint pain. The number of affected joints was mostly more than two(86.7%), and the knee joints(80.0%), hip joints(40.0%), and ankle joints(36.7%) in the lower extremity joints were more common, and they distributed asymmetrically. Twenty-three patients underwent musculoskeletal ultrasound(MSUS) examination on the joints. Their abnormal manifestations were mainly joint cavity effusion and synovitis. Twenty-two patients underwent magnetic resonance imaging(MRI) examination on the joints. The main abnormal finding was joint cavity effusion, with no significant bone destruction observed. Before treatment, the 27-Joint Juvenile Arthritis Disease Activity Scores(JADAS27) of the 30 patients were 9.1(5.4, 12.1)points, indicating that they were in the active stage of the disease. After the standard antibiotic treatment and symptomatic treatment for MP infection(or combined with glucocorticoid treatment), their JADAS27 dropped to 1.2(0.0, 2.4)points, and the difference was statistically significant(Z=4.983, P<0.01). The symptoms of joint damage in 20 patients were completely recovered within 6 months, while the other 10 patients were still under follow-up, and their clinical symptoms were significantly relieved. On the whole, the prognosis of the 30 patients was good, and no complications in other systems were observed. Conclusion　MP infection-associated juvenile arthritis is more common in school age children. The typical symptoms are asymmetric pain in the large joints of the lower extremities. The imaging findings are joint effusion and synovitis as the main abnormal manifestations. Early identification and targeted intervention measures can lead to favorable prognosis for the pediatric patients.]]> 2026/5/29 19:40:21 MA Wenjing, CAO Lanfang 40true ［Abstract］　Objective　To understand the distribution characteristics of serum concentration of valproic acid(VPA) in pediatric patients receiving antiepileptic treatment and to analyze their influencing factors. Methods　A retrospective analysis was conducted on the clinical data of 664 pediatric patients with epilepsy who received sodium valproate treatment in Guangzhou Women and Children′s Medical Center Liuzhou Hospital from January 2021 to June 2024. The serum concentration of VPA in the pediatric patients during the treatment period was determined by using fluorescence immunochromatography(2 514 cases). The effects of factors including age, gender, dosage, dosage form and combination medication on the serum concentration of VPA were analyzed. The correlation between the serum concentration of VPA and its therapeutic effect was analyzed. The occurrence of adverse reactions in the pediatric patients during the treatment period was recorded. Results　The serum concentration of VPA was determined in a total of 2 514 cases, and the average serum concentration of VPA was (58.86±26.11)μg·mL^-1. The serum concentration of VPA ranged from 50 to 100 μg·mL^-1 in 1 419 cases(56.44%), and <50 μg·mL^-1 in 936 cases(37.23%), and >100 μg·mL^-1 in 159 cases(6.32%). Age, dosage, dosage form and combination medication had significant effects on the serum concentration of VPA(P<0.05), while gender had no significant effects on the serum concentration of VPA(P>0.05). The pediatric patients′ serum concentration of VPA were significantly correlated with their therapeutic effects(P<0.05). Among the pediatric patients with the serum concentration of VPA ranging from 50 to 100 μg·mL^-1, the proportion of the pediatric patients with markedly effective medication and with effective medication was 83.16%. The serum concentration of VPA in 39 pediatric patients(a total of 51 cases) exceeded the warning value(120 μg·mL^-1), among whom 2 pediatric patients had liver function injury, and the other pediatric patients had no adverse reactions. Conclusion　Serum concentration of VPA in pediatric patients undergoing antiepileptic treatment is related to their age, dosage, dosage form and combination medication. In clinical practice, the serum concentration of VPA should be monitored regularly to formulate personalized treatment plans and improve therapeutic effects.]]> 2026/5/29 19:40:21 1, XIE Wei¹, FU Lixiang¹, LU Caihong¹, MENG Lidi¹, ZENG Xianzhi²]]> YANG Ni¹, XIE Wei¹, FU Lixiang¹, LU Caihong¹, MENG Lidi¹, ZENG Xianzhi² 39true ［Abstract］　Objective　To analyze the clinical phenotypes and genetic variation characteristics of mitochondrial diseases in children, with the aim of identifying mitochondrial diseases in children as early as possible. Methods　The clinical data of 18 pediatric patients with mitochondrial diseases who were admitted to the Department of Neonatology of the First Affiliated Hospital of Henan Medical University, the Department of Neonatology of Kaifeng Children′s Hospital and the Department of Pediatrics of Dongguan Eighth People′s Hospital(Dongguan Children′s Hospital) from May 2020 to February 2025 were retrospectively collected. These clinical data included gender, age, clinical phenotypes, results of head magnetic resonance imaging(MRI) scans, genetic testing results, treatment and follow-up data. Results　A total of 18 pediatric patients were included, including 12 males and 6 females, among whom 9 pediatric patients developed the disease in the neonatal period. In the 18 pediatric patients, there were 14 cases of primary mitochondrial disease. Among the 14 pediatric patients with primary mitochondrial disease, there were 11 cases of the disease caused by nuclear gene variations. There were 4 cases of secondary mitochondrial disease. The clinical phenotypes of the 18 pediatric patients with mitochondrial diseases were diverse, with hyperlactatemia being the most common(7 cases). The results of head MRI scans suggested that the lesions were mostly involved in the cerebral white matter and subcortical nuclei. All the pediatric patients were given symptomatic treatments, and 4 pediatric patients received cocktail therapy for mitochondrial diseases. During the follow-up, 8 pediatric patients died before the age of 3; 6 pediatric patients had developmental delay; 2 pediatric patients were generally in good condition; 1 pediatric patient had ptosis of both eyelids and 1 pediatric patients had weakness of one lower limb. Conclusion　The phenotypes of mitochondrial diseases in children are not specific, among which hyperlactatemia is a common phenotype. Active genetic testing can confirm the diagnosis, and symptomatic and supportive treatments can improve the prognosis.]]> 2026/5/29 19:40:21 1, TANG Jun², LI Jianwei³]]> CUI Qingyang¹, TANG Jun², LI Jianwei³ 38true ［Abstract］　Objective　To analyze the factors influencing major pathological response(MPR) following neoadjuvant therapy in limited-stage small cell lung cancer(LS-SCLC) patients, and to analyze the factors affecting the patients′ prognosis after surgery. Methods　A retrospective analysis was conducted on the clinical data of 43 patients with LS-SCLC who underwent surgery in Beijing Chest Hospital, Capital Medical University from March 2018 to February 2024. The patients received neoadjuvant therapy before surgery and were divided into MPR group and non-MPR group according to the pathological examination results of the surgical resection specimens. Multivariate logistic regression was used to analyze the factors influencing the LS-SCLC patients to get MPR after neoadjuvant therapy. The differences in overall survival(OS) and disease-free survival(DFS) were compared between the two groups. The factors influencing the recurrence of LS-SCLC in the patients after surgery were analyzed by using univariate Cox regression. Results　Among the 43 patients, 24 patients(55.81%) got MPR, while 19 patients(44.19%) did not. Taking neoadjuvant chemotherapy alone as a reference, neoadjuvant chemotherapy combined with immunotherapy was an influencing factor for getting MPR in the LS-SCLC patients after neoadjuvant therapy(P<0.05). There were no statistically significant differences in OS and DFS between the MPR group and the non-MPR group(log-rank test: χ²=2.579, P=0.324; χ²=2.847, P=0.092). The results of univariate Cox regression analysis revealed that postoperative disease recurrence in the LS-SCLC patients was significantly associated with baseline T stage(P<0.05), but was not associated with gender, age, baseline N stage, baseline neuron-specific enolase(NSE), baseline pro-gastrin-releasing peptide(Pro-GRP), preoperative T stage, preoperative N stage, neoadjuvant therapy regimen, number of neoadjuvant therapy cycles, pathological response to neoadjuvant therapy, and the tumor volume reduction rate after neoadjuvant therapy, surgical approach and the extent of resection(P>0.05). Conclusion　The neoadjuvant therapy regimen is a factor influencing LS-SCLC patients to get MPR after neoadjuvant therapy. Compared with the patients receiving neoadjuvant chemotherapy alone, those receiving neoadjuvant chemotherapy combined with immunotherapy have a higher probability of getting MPR. Baseline T stage is associated with postoperative recurrence in the LS-SCLC patients.]]> 2026/4/30 0:00:00 1, LIU Shuai², LIN Haifeng³, YAO Heng¹, CHENG Yuting¹, CHE Nanying³, YANG Lei¹, MA Teng⁴, ZHI Xiuyi⁵, WANG Chong¹]]> SONG Liwei¹, LIU Shuai², LIN Haifeng³, YAO Heng¹, CHENG Yuting¹, CHE Nanying³, YANG Lei¹, MA Teng⁴, ZHI Xiuyi⁵, WANG Chong¹ 37true ［Abstract］　Objective　To investigate effects of natural product Garcinone C on oxidative damage in nasopharyngeal carcinoma(NPC) cells and the related mechanisms. Methods　NPC cell lines HONE1 and HK1 were cultured in vitro and were intervened with Garcinone C at concentrations of 0 μmol/L, 5 μmol/L and 7.5 μmol/L, respectively. The content of malondialdehyde(MDA), a lipid peroxidation product in cells, was detected by using a microplate reader. The levels of intracellular lipid peroxidation were detected by using flow cytometry combined with BODIPY 581/591 C11 probe staining. Immunofluorescence assay(IFA) was used to detect the expression of γ-H2A.X in the cell nucleus, a marker of DNA damage. Western blot was employed to detect the expression levels of oxidative damage-related proteins, including γ-H2A.X, RAD50, p53 and KEAP1. Results　After the intervention with Garcinone C, the MDA content and lipid peroxidation levels in the HONE1 and HK1 cells were significantly increased(P<0.05), and the expression levels of γ-H2A.X were significantly increased(P<0.05), and the protein expressions of γ-H2A.X and p53 were significantly increased(P<0.05), and the protein expressions of KEAP1 and RAD50 were significantly decreased(P<0.05). Conclusion　Garcinone C exerts anti-tumor effects via inducing oxidative damage in NPC cells and may serve as a promising lead compound for treatment of NPC.]]> 2026/4/30 11:10:44 1, BI Aozhen¹, ZHAO Ming¹, GUO Yulin¹, HU Jingwen¹, YIN Fuqiang², LIU Xia¹]]> ZHAO Wenya¹, BI Aozhen¹, ZHAO Ming¹, GUO Yulin¹, HU Jingwen¹, YIN Fuqiang², LIU Xia¹ 36true ［Abstract］　Objective　To explore the shared characteristics of differentially expressed genes(DEGs) in the two disease models of myocardial infarction(MI) and stroke, and to analyze the cell-type specific distribution of cell death pathways by combining single-cell transcriptomic data, thereby revealing the potential association of cardiovascular and cerebrovascular diseases with mechanisms of programmed cell death. Methods　The GSE66360(MI) and GSE16561(stroke) datasets were downloaded from Gene Expression Omnibus(GEO) database. DEGs analysis was conducted using the limma software package, and the volcano plots were generated. Venn diagram analysis was performed to screen the common DEGs of the two datasets. Enrichment analysis was performed by using clusterProfiler. The key DEGs in the pathways closely related to cardiovascular diseases were further selected, and their correlations with cell death-related gene set were analyzed, and scatter plots were generated. The single-cell transcriptomics data(GSE174574) of the middle cerebral artery occlusion(MCAO) mouse model of stroke were further used to analyze the expression patterns and enrichment characteristics of pyroptosis-related genes in different cell populations. Results　In the GSE66360 and GSE16561 datasets, 1 785 and 561 DEGs were detected, respectively, and ultimately 186 shared DEGs were obtained. Enrichment analysis revealed that the positive regulation of tumor necrosis factors and the pathways of neutrophil extracellular traps(NETs) were closely related to cardiovascular diseases. Correlation analysis showed that the DEGs in the above two pathways were significantly correlated with some cell death genes. The results of single-cell transcriptomics analysis showed that the pyroptosis signals in the stroke mouse model group were mainly enriched in monocytes and microglial cells, and their expression levels were significantly higher than those in the control group. Conclusion　The common DEGs shared by MI and stroke are significantly correlated with the core factors of pyroptosis pathways, and the pyroptosis-related signals are mainly enriched in monocytes and microglial cells.]]> 2026/4/30 0:00:00 1,2, LIU Long^1,2, LI Ruifang³, HAN Yuebing^1,2, LIU Zhijun⁴]]> XU Shuqin^1,2, LIU Long^1,2, LI Ruifang³, HAN Yuebing^1,2, LIU Zhijun⁴ 35true ［Abstract］　Objective　To screen biomarkers related to the efficacy and thoracic radiological toxicity of chemoradiotherapy(CRT) in non-small cell lung cancer(NSCLC) based on genomic maps. Methods　A total of 55 patients with NSCLC who received CRT in Hubei Cancer Hospital from March 2022 to March 2024 were recruited. The serum samples of the patients were collected before and after definitive CRT(dCRT), and cell-free deoxyribonucleic acids(cfDNAs) were extracted for whole exome sequencing(WES). Tumor mutational burden(TMB) and tumor fraction(TF) were calculated. Droplet digital polymerase chain reaction(ddPCR) was used to evaluate the frequencies of mutant alleles. The patients were followed up and their radiation injuries and disease progression were recorded. Results　WES was performed on a total of 110 paired cfDNAs samples in the NSCLC patients, with an average TF of 36.30% before dCRT and 50.30% after dCRT. Somatic single nucleotide variations were positively correlated with TF and tumor diameter(r=0.257, P=0.033; r=0.274, P=0.025). WES showed a strong correlation with variant allele frequencies measured by ddPCR(r=0.971, P<0.001).There were 28 patients receiving concurrent dCRT and 27 patients receiving sequential dCRT in this study. During the treatment, 21 patients(38.18%) experienced toxic reactions ≥grade 2, including 17 cases of radiation pneumonitis and 6 cases of radiation esophagitis. Up to January 2, 2025, the median follow-up time for the patients in this study was 19.6 months(9.0-36.5 months). After excluding 5 patients who abandoned the original treatment plan or were lost to follow-up, a total of 50 patients were included in the prognosis analysis. Thirty-eight patients experienced disease progression, and ultimately 18 patients(36.00%) died due to cancer. The multivariate Cox regression analysis showed that smoking history［HR(95%CI)=2.265(1.019-4.601), P=0.036］, ATM gene mutation［HR(95%CI)=2.074(1.004-4.351), P=0.042］, KEAP1 gene mutation［HR(95%CI)=2.585(1.083-5.425), P=0.014］, and MLL2 gene mutation［HR(95%CI)=3.202(1.272-9.851), P=0.011］ were independent risk factors affecting the disease-free survival of NSCLC patients after dCRT. ZNF217 gene amplification［HR(95%CI)=5.325(1.098-25.827), P=0.038］ and POLD1 gene mutation［HR(95%CI)=9.756(1.670-57.008), P=0.011］ were the independent risk factors of developing chest radiation injuries ≥grade 2. Conclusion　ATM, KEAP1 and MLL2 gene mutations are the genetic features that affect progression-free survival in NSCLC patients after dCRT, while ZNF217 gene amplification and POLD1 gene mutation are associated with radiological toxicity.]]> 2026/4/30 11:10:44 1, WANG Huabin¹, LIANG Gai², XIANG Zhengkai¹]]> LIU Yuanxiang¹, WANG Huabin¹, LIANG Gai², XIANG Zhengkai¹ 34true ［Abstract］　Objective　To compare the effects of Tubridge flow diverter(TFD) and Pipeline embolization device(PED) in treatment of medium to large intracranial vertebral artery dissecting aneurysms(IVADAs). Methods　The clinical data of 87 patients with medium to large IVADAs who received treatment with flow diverter devices in West China Hospital, Sichuan University from January 2019 to December 2024 were retrospectively analyzed. According to the flow diverter devices used, the patients were divided into TFD group(receiving TFD treatment, 42 patients) and PED group(receiving PED treatment, 45 patients). The perioperative related indicators, postoperative aneurysm occlusion and in-stent stenosis rate, clinical prognosis and incidence of perioperative complications were compared between the two groups. Results　There were no significant differences in the operation time, the length of hospital stay after operation and the number of implanted stents between the two groups(P>0.05). There were no statistically significant differences in the O′Kelly-Marotta(OKM) grades immediately after the operation and at the last follow-up between the two groups(P>0.05). There was no statistically significant difference in the in-stent stenosis rate between the two groups at the last follow-up(P>0.05). There were no statistically significant differences in the modified Rankin Scale(mRS) scores between the two groups at discharge from hospital, 1 month after the operation, and at the last follow-up(P>0.05). There was no statistically significant difference in the total incidence of perioperative complications between the two groups［16.67%(7/42) vs 6.67%(3/45); χ²=1.266, P=0.261］. Conclusion　Both TFD and PED can make the patients achieve good clinical prognosis and safety in the treatment of medium to large IVADAs.]]> 2026/4/30 0:00:00 1,2, MA Yuhu¹, SHANG Rui¹, LI Sihao¹, LEI Zhen², QI Xinwei², ZHANG Changwei¹]]> LONG Qiangyou^1,2, MA Yuhu¹, SHANG Rui¹, LI Sihao¹, LEI Zhen², QI Xinwei², ZHANG Changwei¹ 33true ［Abstract］　Objective　To explore the association of serum ALKB homolog 5(ALKBH5) and FK506-binding protein 5(FKBP5) with T helper cells 17(Th17)/regulatory T cells(Tregs) imbalance and tocolysis outcomes in patients with recurrent spontaneous abortion(RSA). Methods　One hundred patients with RSA who were admitted to Handan First Hospital from January 2022 to January 2024 were recruited as RSA group, and 100 healthy pregnant women without an adverse pregnancy history during the same period were selected as control group. According to tocolysis outcomes of the RSA patients, they were divided into successful tocolysis group(67 patients) and failed tocolysis group(33 patients). The levels of serum ALKBH5 and FKBP5 in the research subjects were detected by using enzyme-linked immunosorbent assay(ELISA). The levels of Th17 and Tregs in the peripheral blood were detected by using flow cytometry, and the Th17/Tregs ratio was calculated. Pearson correlation was used to analyze the correlations of serum ALKBH5 and FKBP5 levels with Th17, Tregs and Th17/Tregs ratio levels. Multivariate logistic regression was used to analyze the factors influencing the failed tocolysis in the RSA patients. Receiver operating characteristic(ROC) curve was used to analyze the efficacy of serum ALKBH5 and FKBP5 for predicting the failed tocolysis in the RSA patients. Results　The levels of serum ALKBH5 and Tregs in the RSA group were lower than those in the control group, while the levels of FKBP5, Th17, and Th17/Tregs ratio in the RSA group were higher than those in the control group, with statistically significant differences between the two groups(P<0.05). The results of Pearson correlation analysis showed that in the RSA patients the serum ALKBH5 level was negatively correlated with Th17 and Th17/Tregs ratio(P<0.05), and was positively correlated with Tregs level(P<0.05); serum FKBP5 level was positively correlated with Th17 and Th17/Tregs ratio(P<0.05), and was negatively correlated with Tregs level(P<0.05). The results of multivariate logistic regression analysis showed that higher Th17/Tregs ratio［OR(95%CI)=2.327(1.050-5.157), P=0.038］ and higher FKBP5 level［OR(95%CI)=3.145(1.351-7.320), P=0.008］ were independent risk factors for failed tocolysis in the RSA patients(P<0.05), and higher ALKBH5 level［OR(95%CI)=0.205(0.071-0.594), P=0.004］ was an independent protective factor for inhibiting the occurrence of failed tocolysis in the RSA patients(P<0.05). The results of ROC curve analysis showed that the efficacy of serum ALKBH5 combined with FKBP5 in predicting failed tocolysis in the RSA patients was higher than that of a single indicator(Z_{combination of the two indicators- ALKBH5}=2.092, P=0.036; Z_{combination of the two indicators- FKBP5}=2.451, P=0.014), with a sensitivity of 96.97% and a specificity of 73.13%. Conclusion　Serum ALKBH5 level decreases and serum FKBP5 level increases in RSA patients, and the abnormal expressions of both indicators may be related to Th17/Tregs imbalance and failed tocolysis.]]> 2026/4/30 11:10:44 1, GUO Jie¹, TANG Lili²]]> LI Jing¹, GUO Jie¹, TANG Lili² 32true ［Abstract］　Objective　To compare the efficacy and aesthetic effect of single-port laparoscopic surgery versus conventional three-port laparoscopic surgery in treatment of unilateral inguinal hernia. Methods　A retrospective analysis was performed on the clinical data of patients with unilateral inguinal hernia who were admitted to Jilin Province People′s Hospital from January 2024 to January 2025. Among them, 61 patients underwent single-port laparoscopic transabdominal preperitoneal repair(SPLS-TAPP) and were assigned to the SPLS-TAPP group, while 60 patients underwent conventional three-port laparoscopic transabdominal preperitoneal repair(TPLS-TAPP) and were assigned to the TPLS-TAPP group. Perioperative indicators, postoperative pain conditions, postoperative inflammatory factor levels, postoperative complications, and aesthetic effect were compared between the two groups. Results　The operations were successfully completed in both groups, with no conversion to open surgery and no additional Trocar placement in either group. Compared with the TPLS-TAPP group, the SPLS-TAPP group had longer operation time, earlier time to first ambulation after operation, and shorter postoperative hospital stay, with statistically significant differences(P<0.05). There was no statistically significant difference in intraoperative blood loss between the two groups(P>0.05). The Visual Analogue Scale(VAS) scores at 24 and 48 hours after operation in the SPLS-TAPP group were lower than those in the TPLS-TAPP group, and the number of postoperative analgesic uses in the SPLS-TAPP group was less than that in the TPLS-TAPP group, with statistically significant differences(P<0.05). Postoperative levels of C-reactive protein(CRP), white blood cell count(WBC) and interleukin(IL)-10 in the SPLS-TAPP group were significantly lower than those in the TPLS-TAPP group(P<0.05). No severe complications such as mesh infection, intestinal injury and massive hemorrhage occurred postoperatively in either group. There was no statistically significant difference in the total incidence of postoperative complications between the two groups(P>0.05). The patients′ aesthetic satisfaction scores for incision scars in the SPLS-TAPP group were significantly higher than those in the TPLS-TAPP group(P<0.05). Conclusion　Single-port laparoscopic surgery can facilitate the quick recovery of the patients while ensuring their surgical safety in the treatment of unilateral inguinal hernia and improve the aesthetic effect of incision scars.]]> 2026/4/30 11:10:44 SHAO Jianlin, WANG Bowen 31true ［Abstract］　Objective　To explore the diagnostic efficacy of serum latent-transforming growth factor beta-binding protein 2(LTBP2) and cytoskeleton-associated protein 4(CKAP4) levels in differentiating benign and malignant solitary pulmonary nodules. Methods　A total of 140 patients with solitary pulmonary nodules who were admitted to Dongtai People′s Hospital from March 2021 to March 2024 were recruited. Among them, there were 68 cases of benign lesions(benign group), and 72 cases of malignant lesions(malignant group). During the same period, 100 healthy individuals were recruited as control group. On the early morning of the next day after admission(for the control group, during the health check-up), 4 mL of fasting venous blood was collected from each research subject. The levels of serum LTBP2 and CKAP4 were detected by using enzyme-linked immunosorbent assay(ELISA). The correlation between serum LTBP2 and CKAP4 levels in the malignant group was analyzed by using Pearson correlation. The clinical data were compared between the benign group and the malignant group. The factors influencing the occurrence of malignant lesions of solitary pulmonary nodules were analyzed by using logistic regression. Receiver operating characteristic(ROC) curve was used to analyze the diagnostic efficacy of serum LTBP2 and CKAP4 levels in differentiating benign and malignant solitary pulmonary nodules. Results　The levels of serum LTBP2 and CKAP4 in the benign group and the malignant group were higher than those in the control group, and the levels of the two indicators in the malignant group were higher than those in the benign group, with statistically significant differences(P<0.05). The results of Pearson correlation analysis showed that the serum LTBP2 level was positively correlated with the CKAP4 level in the malignant group(r=0.485, P<0.001). The results of multivariate logistic regression analysis showed that higher levels of serum LTBP2［OR(95%CI)=8.807(2.933-26.446), P<0.001］ and CKAP4［OR(95%CI)=5.281(1.902-14.662), P=0.001］ were independent risk factors for promoting the occurrence of malignant solitary pulmonary nodules. The results of ROC curve analysis showed that the levels of serum LTBP2 and CKAP4 could be effective in differential diagnosis of benign and malignant solitary pulmonary nodules(P<0.05), and the diagnostic efficacy of the combination of the two indicators was better than that of a single indicator(Z_{combination of the two indicators-LTBP2}=2.225, P=0.026; Z_{combination of the two indicators-CKAP4}=1.980, P=0.047), with a sensitivity of 94.44% and a specificity of 75.00%. Conclusion　The detection of serum LTBP2 and CKAP4 levels is helpful for differential diagnosis of benign and malignant solitary pulmonary nodules, and the combined detection of the two indicators has a better diagnostic efficacy than a single indicator.]]> 2026/4/30 11:10:44 LI Yanmei, YAO Jun 30true ［Abstract］　Objective　To understand the prevalence and influencing factors of dyslipidemia among adult residents in Guangxi in 2023, and to provide a scientific basis for formulating prevention and control strategies. Methods　From December 2023 to February 2024, a multi-stage stratified cluster random sampling method was used to select 6 231 permanent residents aged 18 years and above in Guangxi. Questionnaire surveys, physical examinations and laboratory tests were conducted on the selected permanent residents. Chi-square test and multivariate logistic regression analysis were performed on the collected data using R 4.4.2 software. Results　A total of 6 099 respondents were included in this study for analysis, among whom 2 787 respondents had dyslipidemia, with a prevalence rate of dyslipidemia of 45.70%. The age-standardized prevalence rate of dyslipidemia was 45.97%, and the age-standardized prevalence rates of hypercholesterolemia, hypertriglyceridemia, hypo-high-density lipoprotein cholesterolemia and hyper-low-density lipoprotein cholesterolemia were 21.25%, 16.17%, 22.37% and 14.75%, respectively. The results of multivariate logistic regression analysis showed that age range of 45-59 years and ≥60 years, rural residents, daily smoking, overweight and obesity, hypertension and diabetes were risk factors for promoting the occurrence of dyslipidemia(P<0.05), while female was a protective factor for inhibiting the occurrence of dyslipidemia(P<0.05). Conclusion　In 2023, the prevalence rate of dyslipidemia among adults in Guangxi is relatively high. Lifestyle interventions should be focused on males, people over 45 years old, rural residents, smokers, overweight and obese individuals, and people with chronic diseases to control risk factors and reduce the risk of illness.]]> 2026/4/30 11:10:44 1, LUO Shuiying^1*, DENG Huitian², TANG Meiyan³, JIANG Xiaoyun¹, HUANG Jinmei¹, XU Jingjing¹, TENG Youming¹, ZHONG Yanxu^1,4]]> MAO Wei¹, LUO Shuiying^1*, DENG Huitian², TANG Meiyan³, JIANG Xiaoyun¹, HUANG Jinmei¹, XU Jingjing¹, TENG Youming¹, ZHONG Yanxu^1,4 29true ［Abstract］　Objective　To observe the effect of neuromuscular electrical stimulation(NMES) in treatment of postpartum diastasis recti abdominis(DRA) and to analyze its influence on Young′s modulus indices of shear wave elastography(SWE). Methods　A total of 80 patients with postpartum DRA who were admitted to Liuzhou Municipal Liutie Central Hospital from January 2022 to March 2024 were recruited and divided into control group and observation group by random number table method, with 40 patients in each group. The observation group received NMES treatment, while the control group received postpartum abdominal compression belt treatment. The duration of rehabilitation treatment was 10 days for both groups. High-frequency ultrasound(HFUS) was used to measure the values of inter-rectus distance(IRD) 2 cm above the umbilicus, 5 cm above the umbilicus, at the level of the umbilicus and 2 cm below the umbilicus at the resting and flexed states in the patients, and the thickness of the bilateral rectus abdominis muscles was recorded. The Young′s modulus indices of the umbilical plane of rectus abdominis muscles was measured by using SWE. The Young′s modulus indices included the mean value of right tissue elasticity(Emean R), the maximum value of right tissue elasticity(Emax R), the minimum value of right tissue elasticity(Emin R), the mean value of left tissue elasticity(Emean L), the maximum value of left tissue elasticity(Emax L), and the minimum value of left tissue elasticity(Emin L). The clinical efficacy was compared between the two groups. Results　After treatment, the values of IRD 5 cm above the umbilicus, 2 cm above the umbilicus, at the level of the umbilicus and 2 cm below the umbilicus at the resting and flexed states in the patients in the observation group were less than those in the control group, and the thickness of the bilateral rectus abdominis muscles and the Young′s modulus indices of SWE in the observation group were greater than those in the control group, with statistically significant differences(P<0.05). The overall clinical effective rate of the observation group was significantly higher than that of the control group［87.50% vs 65.00%, χ²=5.591, P=0.018］. Conclusion　NMES therapy can effectively improve the morphology and mechanical properties of the rectus abdominis muscles in patients with postpartum DRA. HFUS combined with SWE provides objective indicators for clinical efficacy evaluation.]]> 2026/4/30 11:10:44 PAN Huangui, PAN Yongshou, JIANG Yunxiu, MAI Lihuan, ZHU Shangning, PENG Chunyan 28true ［Abstract］　Objective　To analyze the expressions of immediate early response 3(IER3) in rectal cancer tissues and preoperative circulating tumor cells(CTCs), and to investigate the association of the IER3 expressions with the patients′ clinicopathological characteristics. Methods　A total of 42 patients with rectal cancer who were admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2017 to January 2019 were recruited. Forty-two healthy individuals undergoing physical examination during the same period were also recruited. At 1 day before operation, 5 mL of peripheral blood was collected from each patient and from each healthy individual at the time of physical examination. The preoperative CTCs levels in the blood samples and the expressions of IER3 mRNA in CTCs were detected by using in situ hybridization technology, and the classification of CTCs was performed. The expression levels of IER3 mRNA in rectal cancer tissues and their adjacent tissues were detected by using quantitative real-time polymerase chain reaction(qRT-PCR), and the expressions of IER3 protein in these tissues were detected by using immunohistochemical staining. The association of the expression levels of IER3 mRNA in rectal cancer tissues with the patients′ clinicopathological characteristics was analyzed. The correlation of the expressions of IER3 mRNA and protein in rectal cancer tissues with the levels of CTCs was analyzed. The associations between the levels of CTCs as well as the levels of CTCs with positive expression of IER3 mRNA and the patients′ clinicopathological characteristics were analyzed. The follow-up was conducted until December 31, 2023 to analyze the associations of the expression levels of IER3 mRNA and protein in rectal cancer tissues with the patients′ survival prognosis. Results　The expression level of IER3 mRNA and the positive expression rate of IER3 protein in rectal cancer tissues were significantly higher than those in adjacent tissues(P<0.05). No CTCs were detected in any of the healthy individuals undergoing physical examination. The detection rate of preoperative CTCs in the rectal cancer patients was 92.86%(39/42), among whom 29 patients exhibited IER3 mRNA expression in their CTCs, with an expression rate of 74.36%(29/39). There were no significant differences in the expressions of IER3 mRNA among different types of CTCs(P>0.05). The results of Spearman′s rank correlation analysis showed that the expression level of IER3 mRNA in rectal cancer tissues was negatively correlated with the level of CTCs(r_s=-0.317, P=0.041). The expression of IER3 protein in rectal cancer tissues was significantly correlated with the level of total CTCs and the level of mixed CTCs(P<0.05). The level of CTCs was not significantly correlated with the patients′ clinicopathological characteristics(P>0.05). The level of interstitial CTCs with positive expression of IER3 mRNA was significantly correlated with the patients′ N stage, T stage and TNM stage(P<0.05). The levels of epithelial CTCs, mixed CTCs and total CTCs with positive expression of IER3 mRNA were not significantly correlated with the patients′ clinicopathological characteristics(P>0.05). The expressions of IER3 protein and IER3 mRNA in rectal cancer tissues were not significantly correlated with the patients′ survival prognosis(P>0.05). Conclusion　IER3 is highly expressed in rectal cancer tissues, which is associated with the pathological characteristics related to tumor invasion and metastasis, as well as with the number and subtypes of CTCs.]]> 2026/3/31 0:00:00 1, WEI Chunying², LI Songlin³, WEI Dangsheng⁴, ZHONG Xiaogang⁵]]> ZHOU Rizhong¹, WEI Chunying², LI Songlin³, WEI Dangsheng⁴, ZHONG Xiaogang⁵ 27true ［Abstract］　Objective　To analyze the clinicopathological features of tumors of the three major salivary glands(parotid gland, submandibular gland and sublingual gland), and to provide reference for the diagnosis and treatment of these diseases. Methods　A retrospective analysis was conducted on the pathological data of 656 patients with tumors of the parotid gland, submandibular gland and sublingual gland who were admitted to General Hospital of Southern Theater Command of PLA from January 2000 to December 2020. The classification and statistics of the salivary gland tumors were carried out by referring to the histopathological classification and typing criteria of salivary gland tumors by the World Health Organization(WHO), and the clinicopathological features of the tumors of the three major salivary glands were summarized. Results　Among the 656 patients, 411 patients were male(62.65%) and 245 patients were female(37.35%). The age of the patients at the time of onset was mainly distributed between 35 and 64 years(52.90%). There were 465 cases(70.88%) of benign tumors and 191 cases(29.12%) of malignant tumors. There were 545 cases(83.08%) of tumors occurring in the parotid gland, 97 cases(14.79%) of tumors occurring in the submandibular gland and 14 cases(2.13%) of tumors occurring in the sublingual gland. In the benign tumors, the top 3 tumors in terms of proportion were pleomorphic adenoma(46.88%, 218/465), Warthin tumor(33.33%, 155/465) and basal cell adenoma(5.59%, 26/465), among which pleomorphic adenoma accounted for 33.23%(218/656) of all the tumors. Among the 191 cases of malignant tumors, 143 cases(74.87%) of tumors occurred in the parotid gland, 34 cases(17.80%) of tumors in the submandibular gland and 14 cases(7.33%) of tumors in the sublingual gland. In the malignant tumors, the top 3 tumors in terms of proportion were adenoid cystic carcinoma(25.65%, 49/191), mucoepidermoid carcinoma(18.85%, 36/191) and acinic cell carcinoma(13.09%, 25/191). Conclusion　Among the patients with the tumors of the three major salivary glands, the proportion of male patients is higher than that of female patients. These tumors are more common in the population aged 35 to 64 years. The tumor sites are mainly located in the parotid gland. Among the benign tumors, pleomorphic adenoma accounts for the highest proportion. Among the malignant tumors, adenoid cystic carcinoma accounts for the highest proportion.]]> 2026/3/31 9:16:41 1, OUYANG Qinghua¹, HU Jingran¹, LUO Yirong¹, SUN Shuohui¹, WANG Zhifa^1,2]]> JIAN Lan¹, OUYANG Qinghua¹, HU Jingran¹, LUO Yirong¹, SUN Shuohui¹, WANG Zhifa^1,2 26true ［Abstract］　Objective　To observe the effect of treating pelvic acetabular fractures by small incision approach via the lateral rectus abdominis muscle. Methods　A retrospective analysis was conducted on the clinical data of 86 patients with pelvic acetabular fractures who were admitted to Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology from April 2021 to December 2023. According to different treatment regimens, the patients were divided into observation group(adopting small incision approach via the lateral rectus abdominis muscle) and control group(adopting ilioinguinal approach), with 43 patients in each group. The perioperative indicators, surgical stress indicators, rate of satisfaction with fracture reduction, Majeed Pelvic Scores and the occurrence of complications were compared between the two groups. Results　Compared with the control group, the observation group had shorter time of fracture end exposure, duration of operation and hospital stay, and less intraoperative blood loss, with statistically significant differences between the two groups(P<0.05). On the first day after the operation, the levels of cortisol(Cor) and norepinephrine(NE) in the observation group were significantly lower than those in the control group(P<0.05). The rate of satisfaction with fracture reduction in the observation group was significantly higher than that in the control group(88.37% vs 69.77%; χ²=4.497, P=0.034). The postoperative Majeed Pelvic Scores in both groups showed an upward trend(P<0.05). The Majeed Pelvic Scores in the observation group at the 3rd month after operation were significantly higher than those in the control group(P<0.05), while there were no significant differences in the Majeed Pelvic Scores between the two groups at the 6th month and 12th month after operation(P>0.05). The total incidence of complications in the observation group was lower than that in the control group(4.65% vs 23.26%; χ²=6.198, P=0.013). Conclusion　Compared with the operation by ilioinguinal approach, the operation by small incision approach via the lateral rectus abdominis muscle has the advantages of less trauma and faster postoperative recovery in treating pelvic acetabular fractures, can enhance the rate of satisfaction with fracture reduction, and is beneficial to the recovery of the patient′s early pelvic floor function, with less postoperative complications, and is worthy of clinical recommendation.]]> 2026/3/31 9:16:41 1,2, HE Li², YI Chengla²]]> CHENG Ruixiang^1,2, HE Li², YI Chengla² 25true ［Abstract］　Objective　To analyze the clinical efficacy and influencing factors of cyclophosphamide(CYC) in treatment of anti-synthetase syndrome-associated interstitial lung disease(ASS-ILD). Methods　A retrospective analysis was conducted on the clinical data of 111 patients with ASS-ILD who were admitted to the Third Affiliated Hospital of Nanjing Medical University and the Third Affiliated Hospital of Soochow University from January 2020 to May 2024. According to different initial therapeutic regimens, the patients were divided into CYC group(49 patients) and non-CYC group(62 patients). The patients in the CYC group were divided into anti-histidyl transfer RNA(tRNA) synthetase(Jo-1) antibody negative group and anti-Jo-1 antibody positive group according to their different serum antibody states. The patients in the CYC group were divided into forced vital capacity percent predicted(FVC%pred)<70% group and FVC%pred≥70% group according to their different baselines of lung function(before treatment). The baseline data, the changes in lung function before treatment and 6 to 12 months after treatment, and the 1-year follow-up data were compared between two groups. Results　Compared with the non-CYC group, the CYC group had the significantly elevated anti-Jo-1 antibody positive rate, proportion of myasthenia symptoms and white blood cell count(WBC), hemoglobin(Hb), and creatinine kinase-myocardial band(CK-MB) levels (P<0.05). During the follow-up period, the improvement extent of FVC%pred in the CYC group was greater than that in the non-CYC group, with statistically significant difference between the two groups(P<0.05). In the CYC group, there were no patients with a decrease in FVC%pred≥10% compared to the baseline data, while the proportion of the patients with a decrease in FVC%pred≥10% compared to the baseline data in the non-CYC group was 14.52%. Compared with the non-CYC group, the CYC group had high proportion of drug dosage reduction or withdrawal due to better therapeutic effect, and 1 year after treatment, the average daily dose of prednisone in the CYC group was low, and the differences were statistically significant(P<0.05). Compared with those before CYC treatment, the levels of FVC%pred in the anti-Jo-1 antibody negative group and the anti-Jo-1 antibody positive group were significantly elevated after CYC treatment(P<0.05). There were no statistically significant differences in the levels of diffusing capacity of the lung for carbon monoxide percent predicted(DLCO%pred) between the two groups before and after treatment(P>0.05). After CYC treatment, the improvement extent of FVC%pred in the FVC%pred<70% group was greater than that in the FVC%pred≥70% group, and the difference was statistically significant(P<0.05). Conclusion　CYC has a significantly therapeutic effect on ASS-ILD, especially for progressive patients and severe patients, and can be used as the preferred treatment regimen for these patients.]]> 2026/3/31 0:00:00 1, LI Mingting¹, WU Min², GENG Yaqin¹, ZHOU Lei¹, ZHANG Yu¹, QIAN Hao¹, GAO Bo¹]]> XUE Dingying¹, LI Mingting¹, WU Min², GENG Yaqin¹, ZHOU Lei¹, ZHANG Yu¹, QIAN Hao¹, GAO Bo¹ 24true ［Abstract］　Objective　To construct models for predicting intracranial aneurysm(IA) rupture during craniotomy and clipping based on aneurysm morphological, hemodynamic and general clinical indicators using logistic regression(LR) and extreme gradient boosting(XGBoost) methods, and to verify and compare the predictive efficacy of the models. Methods　A total of 330 patients with IA who were admitted to Xingtai Central Hospital from January 2020 to April 2025 were recruited and randomly divided into training set(231 patients) and verification set(99 patients) at a ratio of 7∶3. All the patients received craniotomy and clipping, and were divided into rupture group and non-rupture group according to whether or not IA ruptured during the operation. Before the operation, computed tomography angiography(CTA) was performed on the patients to obtain the aneurysm morphological indicator and hemodynamic indicator, and the general clinical data of the patients were collected. The models for predicting IA rupture during craniotomy and clipping were constructed by using the LR and XGBoost methods, respectively. The predictive efficacy of the two models was evaluated by using the receiver operating characteristic(ROC) curve and calibration curve. Results　In the training set and the verification set, the proportions of hypertension and intracranial atherosclerosis, and the systemic inflammatory response index(SIRI), the maximum diameter of the aneurysm, the aspect ratio(AR, the long diameter of the aneurysm/the width of the aneurysm neck), and the size ratio(SR, the maximum diameter of the aneurysm/the diameter of the parent artery), the wall shear stress gradient average(WSSGA), and the oscillatory shear index(OSI) in the rupture group were greater than those in the non-rupture group, while the normalized wall shear stress maximum(NWSSM) in the rupture group was less than that in the non-rupture group, and the differences were statistically significant(P<0.05). Based on the data in the training set, the results of LR analysis showed that hypertension, intracranial atherosclerosis, SIRI, AR, SR, OSI and NWSSM were the influencing factors of IA rupture during craniotomy and clipping(P<0.05). The equation of the prediction model: Logit(P)=-1.215+1.096×hypertension+1.329×intracranial atherosclerosis+0.629×SIRI+0.707×AR+0.719×SR+0.838×OSI-0.833×NWSSM. In the prediction model constructed by the XGBoost method, the top 6 factors in terms of the importance of features were OSI, SIRI, SR, NWSSM, AR and intracranial atherosclerosis in sequence. The results of ROC curve analysis based on the training set data showed that both the XGBoost model and the LR model could effectively predict the occurrence of IA rupture during craniotomy and clipping［AUC(95%CI)=0.915(0.875-0.955); AUC(95%CI)=0.838(0.780-0.897)］. The results of calibration curve analysis showed that the calibration degrees of the XGBoost model and the LR model were 0.885 and 0.828, respectively, and the consistency indices were 0.879 and 0.812, respectively. The analytical results of the predictive efficacy of the models based on the verification set data also showed that the XGBoost model had better predictive efficacy and accuracy than the LR model. Conclusion　The XGBoost model based on 6 indicators including OSI, SIRI, SR, NWSSM, AR and intracranial atherosclerosis can effectively predict the occurrence of IA rupture during craniotomy and clipping, which helps clinicians identify high-risk patients early and improve their prognosis.]]> 2026/3/31 9:16:41 WANG Shengjun, FENG Yumin, LI Yan, HAO Jinmin 23true ［Abstract］　Objective　To analyze the effects of homocysteine(Hcy), high-sensitivity C-reactive protein to albumin ratio(hs-CRP/ALB) and triglyceride-glucose(TyG) index on the occurrence of progressive ischemic stroke(PIS), and to construct a nomogram model. Methods　A retrospective analysis was conducted on the clinical data of 331 patients with acute ischemic stroke(AIS) who were admitted to the Department of Neurology of Bayannur Hospital from January 2020 to December 2020. According to the disease progression of the patients during hospitalization, they were divided into PIS group(92 patients) and non-PIS group(239 patients). Multivariate logistic regression was used to analyze the factors influencing the occurrence of PIS, and a nomogram model for predicting the occurrence of PIS was constructed based on the indicators obtained through screening. The predictive efficacy and application value of the nomogram model were evaluated by using receiver operating characteristic(ROC) curve, Hosmer-Lemeshow test, calibration curve and decision curve analysis(DCA). Results　The results of multivariate logistic regression analysis showed that, taking small artery occlusion type as the reference, large artery atherosclerosis type［OR(95%CI)=6.894(3.306-14.375)］ was an independent risk factor promoting the occurrence of PIS(P<0.05); and taking mild vascular stenosis as the reference, severe vascular stenosis［OR(95%CI)=5.105(2.168-12.018)］ and vascular occlusion［OR(95%CI)=18.599(4.696-73.671)］ were independent risk factors promoting the occurrence of PIS(P<0.05); and higher National Institutes of Health Stroke Scale(NIHSS) scores at admission［OR(95%CI)=1.146(1.032-1.271)］, higher Hcy［OR(95%CI)=1.027(1.003-1.051)］, higher hs-CRP/ALB［OR(95%CI)=1.064(1.029-1.101)］ and higher TyG index［OR(95%CI)=1.520(1.044-2.214)］ as well as a history of diabetes［OR(95%CI)=2.631(1.294-5.348)］ were independent risk factors for promoting the occurrence of PIS(P<0.05). Based on the above indicators, a nomogram model for predicting the occurrence of PIS was constructed. The results of ROC curve analysis showed that the constructed model had high predictive efficacy［AUC(95%CI)=0.910(0.872-0.948)］, with a sensitivity of 85.87% and a specificity of 87.87%. The results of Hosmer-Lemeshow test showed that there was no significant difference between the predicted value of the model and its actual observed value(P=0.300). The results of calibration curve analysis showed that the calibration curve of the model was in good consistency with its ideal curve. The results of DCA showed that when the threshold probability values ranged from 0.05 to 0.99, the net benefit of using the model to predict the occurrence of PIS in the AIS patients was relatively high. Conclusion　Higher levels of Hcy, hs-CRP/ALB and TyG index are risk factors promoting the occurrence of PIS. The nomogram model constructed in this study can effectively predict the occurrence of PIS, which is helpful for clinicians to identify patients at high risk as soon as possible.]]> 2026/3/31 9:16:42 1, HE Wen²]]> QING Yun¹, HE Wen² 22true ［Abstract］　Objective　To explore the effects of binocular visual perception training on binocular visual function and stereoscopic vision of pediatric patients after operation for intermittent exotropia(IXT). Methods　A total of 132 pediatric patients with IXT who were admitted to Liuzhou Red Cross Hospital from October 2020 to January 2024 were recruited. They were divided into observation group(receiving binocular visual perception training) and control group(receiving conventional training) by using random number table method, with 66 cases in each group. Both groups were continuously trained for 3 months. The therapeutic effect of the training was compared between the two groups. The binocular visual function was compared between the two groups before operation and after the completion of training. The horizontal fusion range and the proportions of the pediatric patients with distant stereoscopic vision and Titmus stereoscopic vision were compared between the two groups before training(on the first day after operation) and after the completion of training. Results　The total effective rate of the observation group was higher than that of the control group, with statistically significant difference between the two groups(95.45% vs 78.79%; χ²=8.170, P=0.004). Compared with those before operation, the proportions of the pediatric patients with binocular visual function grade Ⅲ in both groups were significantly increased after the completion of training(P<0.05), and the proportion of the pediatric patients with binocular visual function grade Ⅲ in the observation group was higher than that in the control group after the completion of training, with statistically significant differences(P<0.05). Compared with those before training, the horizontal fusion range and the proportions of the pediatric patients with distant stereoscopic vision and Titmus stereoscopic vision in the two groups were significantly increased after the completion of training(P<0.05). The proportions of the pediatric patients with distant stereoscopic vision and Titmus stereoscopic vision in the observation group after the completion of training were significantly higher than those in the control group(P<0.05), but there was no statistically significant difference in the horizontal fusion range between the two groups(P>0.05). Conclusion　Binocular visual perception training can effectively improve the horizontal fusion range, binocular visual function and stereoscopic vision of pediatric patients after operation for IXT, and therefore improve the effect of surgical treatment.]]> 2026/3/31 9:16:42 LYU Bi, WANG Huanyan, WEI Xianghui, LIANG Honggang, HUANG Hansheng, QIN Lulu 21true ［Abstract］　Objective　To analyze the effects of constraint-induced aphasia therapy(CIAT) combined with repetitive transcranial magnetic stimulation(rTMS) on aphasia quotient(AQ) and language communication skill in post-stroke aphasia(PSA) patients. Methods　A total of 160 patients with PSA who were admitted to Shaanxi Rehabilitation Hospital from March 2021 to July 2023 were recruited as the research subjects. The patients were divided into constraint induction group(80 patients) and combination group(80 patients) by using random number table method. The constraint induction group received CIAT. The combination group received rTMS plus CIAT. Both groups were treated continuously for 4 weeks. The changes in Western Aphasia Battery(WAB) scores, aphasia quotient(AQ) index, scores of the communication sub-item in the Functional Independence Measure Scale(FIM), Communication Activities of Daily Living(CADL) scores, and Montreal Cognitive Assessment(MoCA) scores in the patients in the two groups were observed before and after treatment. The clinical efficacy was compared between the two groups. Results　After treatment, the scores of spontaneous speech, repetition, listening comprehension and naming in the WAB, and the AQ index in both groups were higher than those before treatment, and the above indicators in the combination group were higher than those in the constraint induction group after treatment, with statistically significant differences(P<0.05). After treatment, the scores of understanding and expression in the FIM, and the CADL scores and MoCA scores in the two groups were higher than those before treatment, and the above indicators in the combination group were higher than those in the constraint induction group after treatment, with statistically significant differences(P<0.05). The total effective rate of the combination group was significantly higher than that of the constraint induction group(93.75 % vs 82.50%; χ²=4.838, P=0.028). Conclusion　CIAT combined with rTMS has a significant therapeutic effect on PSA. The combination therapy can improve the patients′ AQ, speech function, daily communication ability, and cognitive function.]]> 2026/3/31 9:16:42 1, JIN Yanchun², ZHANG Yu³]]> WANG Yajie¹, JIN Yanchun², ZHANG Yu³ 20true ［Abstract］　Objective　To study the associations of serum microRNA-210(miRNA-210), microRNA-124(miRNA-124) and microRNA-195(miRNA-195) levels with magnetic resonance imaging(MRI) total load scores and cognitive function in cerebral small vessel disease(CSVD) patients. Methods　A total of 90 patients with CSVD who were admitted to Ningde Municipal Hospital of Ningde Normal University from March 2023 to December 2024 were selected as research group. Thirty healthy individuals who received physical examination in the hospital during the same period were selected as control group. The levels of serum miRNA-210, miRNA-124 and miRNA-195 were detected by using real-time fluorescence quantitative polymerase chain reaction(RT-qPCR). The research group received MRI examination. According to MRI total load scores, the patients were divided into the mild load group(0 pointsResults　The levels of miRNA-210, miRNA-124 and miRNA-195 in the research group were significantly lower than those in the control group(P<0.05). The levels of miRNA-210, miRNA-124 and miRNA-195 in the moderate-to-severe load group were significantly lower than those in the mild load group(P<0.05). The levels of miRNA-210, miRNA-124 and miRNA-195 in the cognitive dysfunction group were significantly lower than those in the normal cognitive function group(P<0.05). The results of Spearman rank correlation analysis showed that the levels of miRNA-210, miRNA-124 and miRNA-195 were negatively correlated with the MRI total load scores［Their correlation coefficients(r_s) were -0.529, -0.692 and -0.536, respectively, P<0.001］, and were positively correlated with the MMSE scores(Their r_s were 0.659, 0.672 and 0.620, respectively, P<0.001). The results of multivariate logistic regression analysis showed that higher levels of miRNA-210, miRNA-124 and miRNA-195 were protective factors for inhibiting cognitive dysfunction in the CSVD patients(P<0.05). The results of ROC curve analysis showed that miRNA-210, miRNA-124 and miRNA-195 could effectively diagnose cognitive dysfunction in the CSVD patients(P<0.05), and the combined diagnostic efficacy of the three indicators［AUC(95%CI)=0.964(0.902-0.992)］ was higher than that of a single indicator(P<0.001), with a sensitivity of 96.77% and a specificity of 78.14%. Conclusion　The levels of serum miRNA-210, miRNA-124 and miRNA-195 in CSVD patients are significantly down-regulated, which are significantly correlated with MRI total load scores and cognitive function. The combined detection of the three indicators can effectively diagnose cognitive dysfunction in the CSVD patients.]]> 2026/3/31 9:16:42 ZHOU Guangning, ZHENG Jingjing 19true ［Abstract］　Objective　To explore the value of color Doppler ultrasound(CDU) combined with the detection of serum Golgi protein 73(GP73) and mucin 2(MUC2) in differentiating benign and malignant breast masses. Methods　A total of 132 female patients with breast tumors who were admitted to the Second Outpatient Department of the Directly Subordinate Agency of Hebei Provincial Government and underwent surgical treatment from February 2022 to February 2024 were selected. According to the postoperative pathological results of the patients, they were divided into benign breast lesion group(53 patients) and breast cancer group(79 patients). All the patients underwent CDU examination before surgery. The pulsatility index(PI), maximum blood flow velocity(Vmax) and blood flow resistance index(RI) in the tumors were compared between the two groups. The levels of serum GP73 and MUC2 were determined by using enzyme-linked immunosorbent assay(ELISA). The diagnostic value of serum GP73 and MUC2 levels for breast cancer was analyzed by using receiver operating characteristic(ROC) curve. Kappa identity test was used to evaluate the consistency of the diagnostic results by using CDU, serum GP73, serum MUC2 and their combination with the pathological diagnostic results. Results　Compared with the benign breast lesion group, the breast cancer group had higher proportions of breast masses with irregular shapes, blurred boundaries and blood flow, and higher PI, RI and Vmax, and higher levels of serum GP73 and MUC2, with statistically significant differences between the two groups(P<0.05). The results of ROC curve analysis showed that both serum GP73 and MUC2 levels could effectively diagnose breast cancer(P<0.05). The analysis on the consistency of the diagnostic results by using CDU, serum GP73 and serum MUC2 alone with the pathological diagnostic results showed that their Kappa values were 0.710, 0.517 and 0.607, respectively(P<0.05). The analysis on the consistency of the diagnostic results by using CDU combined with serum GP73 and MUC2 with the pathological diagnostic results showed that the Kappa value was 0.858(P<0.05). The sensitivity, accuracy and negative predictive value of CDU combined with serum GP73 and MUC2 in diagnosing breast cancer were significantly higher than those of a single indicator(P<0.05). Conclusion　The levels of serum GP73 and MUC2 in breast cancer patients are elevated, and the sensitivity, accuracy and negative predictive value of CDU combined with serum GP73 and MUC2 in diagnosing breast cancer are relatively high, which help to improve the early detection rate of breast cancer.]]> 2026/3/31 9:16:42 1, ZHU Dongpo², SUN Yijie³]]> WANG Ying¹, ZHU Dongpo², SUN Yijie³ 18true ［Abstract］　Objective　To observe the effect of activating Qi and promoting blood circulation with round-sharp needles stimulating meridian points combined with small needle-knife in treatment of de Quervain′s tenosynovitis(DQT). Methods　A total of 106 patients with DQT who were admitted to the Second Affiliated Hospital of Heilongjiang University of Chinese Medicine from February 2022 to February 2024 were recruited. These patients were randomly divided into the observation group(receiving the treatment of activating Qi and promoting blood circulation with round-sharp needles stimulating meridian points combined with small needle-knife) and the control group(receiving small needle-knife treatment) by using random number table method, with 53 patients in each group. The treatment course was 1 month for both groups. The traditional Chinese medicine(TCM) syndrome scores and the levels of wrist joint function indicators were compared between the two groups before and after the treatment. The occurrence of adverse reactions and the recurrence of DQT within 6 months after the treatment were compared between the two groups. Results　After the treatment, the TCM syndrome scores in both groups were significantly lower than those before the treatment(P<0.05), and the TCM syndrome scores in the observation group were lower than those in the control group after the treatment, with statistically significant differences(P<0.05). After the treatment, the Cooney scores in both groups were significantly increased compared with those before the treatment(P<0.05), and the ranges of motions of flexion and extension, ulnar deviation and radial deviation of the wrist joint were significantly great compared with those before the treatment(P<0.05). After the treatment, the Cooney scores in the observation group were higher than those in the control group, and the ranges of motions of flexion and extension, ulnar deviation and radial deviation of the wrist joint in the observation group were greater than those in the control group, and the differences were statistically significant(P<0.05). There was no statistically significant difference in the total incidence of adverse reactions between the two groups(5.66% vs 1.89%; χ²=0.260, P=0.610). There was no statistically significant difference in the recurrence rate between the two groups(3.77% vs 0.00%; P=0.495). Conclusion　Activating Qi and promoting blood circulation with round-sharp needles stimulating meridian points combined with small needle-knife is safe and effective in treatment of de DQT. The combination treatment can significantly improve the wrist joint function of the patients.]]> 2026/2/28 11:50:11 1, XU Xilin²]]> LIU Guangyu¹, XU Xilin² 17true ［Abstract］　Objective　To explore the protective effect and mechanism of reduced glutathione(GSH) on a mouse model of pristane-induced systemic lupus erythematosus-associated diffuse alveolar hemorrhage(SLE-DAH). Methods　In vivo experiments: Female C57BL/6J mice aged 6 to 10 weeks were selected and used to construct a diffuse alveolar hemorrhage(DAH) model by intraperitoneal injection of 0.5 mL pristane. Subsequently, these mice were intraperitoneally injected with GSH on the third day, the 6th day, the 9th day and the 12th day after constructing the model to observe the gross changes in the lungs, pathological changes in lung tissues, and changes in body weight of the mice. The levels of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), and interleukin-1β(IL-1β) in the bronchoalveolar lavage fluid(BALF) of the mice were detected. In vitro experiments: Mouse lung epithelial(MLE)-12 cells were treated with β-CD-pristane and the morphology and density of the cells were observed, and the viability of the cells was assessed. The changes in gene expressions were analyzed by using miRNA-sequencing(mRNA-seq), and the cell proliferation and the level of reactive oxygen species(ROS) were detected after GSH intervention. Results　After the treatment with pristane, the lungs of the mice showed the changes in different degrees of hemorrhage on the whole, and the percentage of gross lung hemorrhage in the lung tissues and DAH scores were significantly increased(P<0.05), with disordered lung tissue structure, significant infiltration of inflammatory cells and significantly decreased body weight(P<0.05), and the levels of TNF-α, IL-6, IL-1β in BALF were significantly increased(P<0.05). After the treatment with β-CD-pristane, the morphology of MLE-12 cells changed, and their viability was significantly decreased(P<0.05). The combination of RNA sequencing(RNA-seq) with Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis and Gene Ontology(GO) functional enrichment analysis suggested that β-CD-pristane might cause cell damage through glutathione metabolism pathways and inflammatory response. After the intervention with GSH, the proliferation of MLE-12 cells was restored(P<0.05), and the intracellular ROS levels were significantly decreased(P<0.05), and the percentage of gross lung hemorrhage in pristane-induced DAH mice and DAH scores were significantly decreased(P<0.05), and the body weight was significantly increased(P<0.05), and the levels of TNF-α, IL-6 and IL-1β in BALF were significantly decreased(P<0.05). Conclusion　GSH intervention can alleviate pristane-induced DAH and inflammatory response in mice. The mechanism of action may be associated with the inhibition of oxidative stress in alveolar epithelial cells and the promotion of proliferation of alveolar epithelial cells.]]> 2026/2/28 0:00:00 1, ZHOU Ying¹, LU Conghua², SHI Chenjun², LIN Caiyu², LI Li², DAI Huanzi¹]]> LIAN Shaopan¹, ZHOU Ying¹, LU Conghua², SHI Chenjun², LIN Caiyu², LI Li², DAI Huanzi¹ 16true ［Abstract］　Objective　To explore the role and mechanism of regulatory B cells(Bregs) in progression of diabetic retinopathy(DR). Methods　Forty male C57/BL6 mice aged 8 weeks were selected and randomly divided into control group and DR group, with 20 mice in each group. The mice in the DR group were intraperitoneally injected with streptozotocin(STZ) at a dose of 30 mg/kg to establish the DR model. The mice in the control group were intraperitoneally injected with the same volume of sodium citrate solution. At the age of 28 weeks, the fundi of the mice were observed by using fundus photography, optical coherence tomography(OCT) and fluorescein fundus angiography(FFA). The spleen and peripheral blood samples of these mice were collected and the levels of Bregs were detected by using flow cytometry. Sixteen patients with DR who were admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region from April 2020 to August 2024 were recruited as the case group, among whom 7 patients had proliferative diabetic retinopathy(PDR) and 9 patients had non-proliferative diabetic retinopathy(NPDR), and 12 age-matched healthy individuals undergoing physical examination during the same period were recruited as the healthy control group. The plasma levels of interleukin-10(IL-10), tumor growth factor-β(TGF-β) and programmed death-ligand-1(PD-L1) were detected by using enzyme-linked immunosorbent assay(ELISA), and the correlations between these indicators and the severity of the disease were analyzed. Results　Fundus color photography showed that no typical microangiomas or hemorrhagic exudates were observed in the retinas of the mice in both the DR group and the control group. OCT examination revealed the presence of localized hyperreflective foci in front of the optic papilla in the mice of the DR group. The FFA examination results showed that the microcirculation of the retinas in the mice of the DR group was abnormal, specifically manifested as the formation of abnormal arteriovenous anastomoses, which was consistent with the early vascular pathological characteristics of DR lesions. The detection results of flow cytometry showed that the levels of Bregs in the spleen and peripheral blood of the mice in the DR group were significantly lower than those in the control group(P<0.05). The detection results of ELISA showed that the plasma levels of IL-10, TGF-β, and PD-L1 in the case group were significantly lower than those in the healthy control group(P<0.05). The levels of plasma IL-10 and PD-L1 in the PDR group were significantly lower than those in the NPDR group(P<0.05), while there was no statistically significant difference in the plasma TGF-β level between the two groups(P>0.05). Conclusion　Bregs are involved in the occurrence and progression of DR by regulating inflammatory responses.]]> 2026/2/28 11:50:11 1,2, CUI Ling², WANG Hao², DENG Wen², CHEN Lifei², XU Fan², TANG Fen², ZHONG Haibin²]]> KE Diyang^1,2, CUI Ling², WANG Hao², DENG Wen², CHEN Lifei², XU Fan², TANG Fen², ZHONG Haibin² 15true ［Abstract］　Objective　To study the mechanisms of action of Daotan decoction in treatment of hypertension based on network pharmacology and molecular docking. Methods　The active ingredients of Daotan decoction, potential targets and hypertension targets were obtained through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP) and GeneCards database. The intersection of the potential action targets of Daotan decoction and the hypertension targets were used for analysis. The active ingredients of Daotan decoction-hypertension target interaction network and protein-protein interaction(PPI) network were constructed. The main active ingredients and core targets of action were screened. The potential mechanisms of action of Daotan decoction in treatment of hypertension were explored via Gene Ontology(GO) functional analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis. The binding ability of the main active ingredients to the core targets was analyzed by molecular docking method. Results　There were a total of 110 intersection targets between the action targets of Daotan decoction and the hypertension targets. A total of 15 active ingredients with degree values ≥20 were extracted from the active ingredients of Daotan decoction-hypertension target interaction network. Among the 110 intersection targets, 109 targets were involved in the PPI network, and 26 action targets whose degree value, betweenness, and centrality all exceeded the average values were identified and presumed to be the core targets of Daotan decoction in treating hypertension. The results of GO functional analysis revealed that 146 enrichment results were obtained from the 110 intersecting targets(P<0.05), including 89 biological processes(BP), 42 molecular functions(MF), and 15 cellular components(CC). A total of 95 related signaling pathways were identified by using KEGG pathway enrichment analysis(P<0.05). The molecular docking results showed that the binding energy values of β-sitosterol, cavidine, stigmasterol and luteolin to tumor necrosis factor(TNF), interleukin-6(IL-6), prostaglandin-endoperoxide synthase 2(PTGS2), and estrogen receptor 1(ESR1) were all below 0 kcal/mol. Conclusion　Daotan decoction has the characteristics of multi-component, multi-target and multi-pathway synergistic effects in treatment of hypertension.]]> 2026/2/28 11:50:11 1,2, FANG Chi¹, LI Su³, YONG Sunan^1,2]]> LONG Yuanxiong^1,2, FANG Chi¹, LI Su³, YONG Sunan^1,2 14true ［Abstract］　Objective　To study the associations of expressions of micro ribonucleic acid-23b-3p(miR-23b-3p) and micro ribonucleic acid-218-5p(miR-218-5p) in cervical squamous cell carcinoma(CSCC) tissues with epithelial-mesenchymal transition(EMT), clinicopathological characteristics and prognosis. Methods　A total of 121 patients with CSCC who were admitted to Yancheng Third People′s Hospital from June 2016 to June 2019 were selected as the research subjects. During the operation, the CSCC tissues and para-carcinoma tissues(more than 5 cm away from the carcinoma tissues) of these patients were collected, and the expressions of miR-23b-3p, miR-218-5p, epithelial cadherin(E-cadherin) and vimentin were detected by using reverse transcription-quantitative polymerase chain reaction(RT-qPCR). The patients were followed up for 5 years after the operation, and their survival prognosis was recorded. Pearson correlation was used to analyze the correlations between the indicators. Survival curves were plotted by using Kaplan-Meier method, and log-rank test was used for comparison between the groups. Cox regression was used to analyze the factors influencing the prognosis of the CSCC patients. Results　The expressions of miR-23b-3p, miR-218-5p and E-cadherin in the CSCC tissues were significantly lower than those in the para-carcinoma tissues(P<0.05), while the expression of vimentin in the CSCC tissues was significantly higher than that in the para-carcinoma tissues(P<0.05). In the CSCC tissues, the expressions of miR-23b-3p and miR-218-5p were negatively correlated with the expression of vimentin(P<0.05), and positively correlated with the level of E-cadherin(P<0.05). The expressions of miR-23b-3p and miR-218-5p were correlated with International Federation of Gynecology and Obstetrics(FIGO) stage, degree of differentiation, depth of myometrial invasion and lymph node metastasis(P<0.05), but were not correlated with age and tumor diameter(P>0.05). The survival prognosis of the patients with high expression of miR-23b-3p(relative expression level ≥1.85, 58 cases) was better than that of the patients with low expression of miR-23b-3p(relative expression level <1.85, 61 cases)(log-rank test: χ²=7.720, P=0.006). The survival prognosis of the patients with high expression of miR-218-5p(relative expression level ≥1.34, 59 cases) was better than that of the patients with low expression of miR-218-5p(relative expression level <1.34, 60 cases)(log-rank test: χ²=5.611, P=0.018). The results of multivariate Cox regression analysis showed that lymph node metastasis was an independent risk factor for death in the patients(P<0.05), and high expressions of E-cadherin, miR-23b-3p and miR-218-5p were protective factors inhibiting death in the patients(P<0.05). Conclusion　Expressions of miR-23b-3p and miR-218-5p are down-regulated in CSCC tissues, and low expressions of miR-23b-3p and miR-218-5p are associated with poor differentiation, advanced FIGO stage, increased depth of myometrial invasion, lymph node metastasis and poor prognosis in CSCC.]]> 2026/2/28 11:50:11 LIU Shanshan, LIU Dan, SHAN Jinmei, WANG Feng 13true ［Abstract］　Objective　To observe the clinical effect of spinal fine-tuning manipulation combined with neck three-needle technique in treatment of neck-type cervical spondylosis(NTCS). Methods　A total of 132 patients with NTCS who were admitted to Handan Hospital of Integrated Traditional Chinese Medicine and Western Medicine from October 2022 to December 2023 were recruited and divided into observation group and control group by using random number table method, with 66 patients in each group. The control group was treated with spinal fine-tuning manipulation, while the observation group was treated with spinal fine-tuning manipulation combined with neck three-needle technique, 3 times weekly, 1 week as a course of treatment. Both groups were treated continuously for 4 courses of treatment. The clinical effective rate, pain condition, cervical spine function, cervical ranges of motions, serum nitric oxide(NO) and endothelin(ET) levels, and relapse rate were compared between the two groups. The occurrence of adverse reactions in the patients during the treatment was recorded. Results　The total clinical effective rate of the observation group was significantly higher than that of the control group(93.94% vs 74.24%; χ²=9.570, P=0.002). After the treatment, the Visual Analogue Scale(VAS) scores and Neck Disability Index(NDI) scores in the observation group were significantly lower than those in the control group(P<0.05), and the cervical ranges of motions including cervical anterior flexion, backward extension, left-handed rotation and right-handed rotation in the observation group were significantly greater than those in the control group(P<0.05). After the treatment, the serum ET level in the observation group was lower than that in the control group, and the serum NO level in the observation group was higher than that in the control group, with statistically significant differences between the two groups(P<0.05). After the treatment, the Activities of Daily Living(ADL) scale scores in the observation group were significantly higher than those in the control group(P<0.05). There was no statistically significant difference in the total incidence of adverse reactions between the two groups(9.09% vs 3.03%; χ²=1.198, P=0.274). At 6 months after the treatment, the relapse rate in the observation group was lower than that in the control group, with statistically significant difference between the two groups(8.06% vs 26.53%; χ²=6.869, P=0.009). Conclusion　The combination of spinal fine-tuning manipulation and neck three-needle technique is safe and effective in treatment of NTCS, and is worthy of clinical recommendation.]]> 2026/2/28 11:50:11 1, FAN Changhai²]]> YUAN Lei¹, FAN Changhai² 12true ［Abstract］　Objective　To analyze the serum microribonucleic acid(miR)-515-5p and homeobox protein B13(HOXB13) levels in non-small cell lung cancer(NSCLC) patients and their associations with clinicopathological features and prognosis. Methods　A total of 118 patients with NSCLC who were admitted to the First People′s Hospital of Foshan from July 2019 to July 2021 were recruited as research group, and 118 healthy individuals who underwent physical examination during the same period were selected as control group. The levels of relative expressions of serum miR-515-5p and HOXB13 mRNA were detected by using real-time fluorescence quantitative polymerase chain reaction(RT-qPCR). The patients were followed up for 3 years after discharge from the hospital. According to the survival status of the patients during the follow-up period, they were divided into poor prognosis group(51 patients) and good prognosis group(67 patients). Pearson correlation was used to analyze the correlation between miR-515-5p level and HOXB13 level. The survival curve was plotted by using Kaplan-Meier method, and the inter-group comparison was conducted by using log-rank test. Cox regression was used to analyze the influencing factors of death in the NSCLC patients. Results　The level of miR-515-5p in the research group was significantly lower than that in the control group(P<0.05), while the level of HOXB13 in the research group was significantly higher than that in the control group(P<0.05). The results of Pearson correlation analysis showed that the miR-515-5p level in the NSCLC patients was negatively correlated with the HOXB13 level(r=-0.742, P<0.001). The levels of miR-515-5p and HOXB13 in the NSCLC patients were related to TNM stage, degree of differentiation and lymph node metastasis(P<0.05), but were not significantly related to age, gender, pathological type and tumor diameter(P>0.05). The serum miR-515-5p level in the poor prognosis group was significantly lower than that in the good prognosis group(P<0.05), while the serum HOXB13 level in the poor prognosis group was significantly higher than that in the good prognosis group(P<0.05). The survival prognosis of the patients with high expression of miR-515-5p(≥0.50) was significantly better than that of the patients with low expression of miR-515-5p(<0.50)(log-rank test: χ²=36.627, P<0.001). The survival prognosis of the patients with low expression of HOXB13(<2.08) was significantly better than that of the patients with high expression of HOXB13(≥2.08)(log-rank test: χ²=12.755, P<0.001). The results of multivariate Cox regression analysis showed that high expression of HOXB13, TNM stages Ⅲ-Ⅳ, low degree of differentiation, and lymph node metastasis were risk factors for death in the patients(P<0.05), and high expression of miR-515-5p was a protective factor for inhibiting death in the patients(P<0.05). Conclusion　Serum miR-515-5p level is decreased and serum HOXB13 level is increased in NSCLC patients. Both miR-515-5p and HOXB13 are related to the clinicopathological features and prognosis of the patients.]]> 2026/2/28 11:50:11 WEI Xiaoqun, CHEN Yaofeng, LAI Qiting, LIU Jian, ZHANG Peifang 11true ［Abstract］　Objective　To explore the value of Fisher′s discriminant function model constructed using computed tomography(CT) parameters in differential diagnosis of thymoma and anterior mediastinal cyst. Methods　The clinicopathological data of patients with anterior mediastinal cysts(including thymic cysts and bronchial cysts) or thymoma proven by pathology examination after surgical resection of the cysts in Department of Thoracic Surgery, Xiangya Hospital of Central South University from January 2015 to June 2025 were retrospectively collected. The ratios of plain scan and enhanced CT values of the tumors to the CT values of the aorta were calculated. One-way analysis of variance combined with Bonferroni′s correction was used for inter-group comparisons. The Fisher′s discriminant function was established based on the screened CT parameters and was verified. Results　A total of 236 patients were included in this study, including 159 cases of thymoma(thymoma group), 54 cases of thymic cysts(thymic cyst group), and 23 cases of bronchial cysts(bronchial cyst group). There was no statistically significant difference in age among the three groups(P>0.05). Compared with those in the thymoma group and the thymic cyst group, the proportion of female patients was higher(P<0.001), and the maximum diameter of tumors was smaller(P<0.001) in the bronchial cyst group, and the proportion of lobulated tumors in the thymoma group was significantly higher than that in the thymic cyst group and the bronchial cyst group(P<0.001). The clarity rate of fat space in the thymoma group was significantly lower than that in the thymic cyst group and the bronchial cyst group(P<0.001). The plain and enhanced CT values and the ratios of plain and enhanced CT values to aortic CT values in the thymoma group and the bronchial cyst group were significantly higher than those in the thymic cyst group(P<0.001), and the enhanced CT value and the ratio in the bronchial cyst group were lower than those in the thymoma group(P<0.001). The enhanced CT values in the thymic cyst group and the bronchial cyst group were significantly lower than those in the thymoma group(P<0.001). The established Fisher′s discriminant functions: D1=0.245×gender(0=female, 1=male)+0.782×tumor shape(1=unlobulated, 2=lobulated)+0.644×fat space(0=blur, 1=clear)-0.344×enhanced CT value+97.228×aortic enhanced CT ratio+0.059×CT enhancement value-9.357; D2=0.970×gender(0=female, 1=male)+0.583×tumor shape(1=unlobulated, 2=lobulated)+0.903×fat space(0=blur, 1=clear)-0.094×enhanced CT value+4.749×aortic enhanced CT ratio+0.110×CT enhancement value+0.546. The cross-validation results showed that the overall diagnostic accuracy rate of the model in differentiating thymoma from anterior mediastinal cyst was 95.76%. Conclusion　The discriminant function diagnostic model established according to the CT parameters has a high diagnostic value in differentiating thymoma from anterior mediastinal cyst.]]> 2026/2/28 0:00:00 YANG Yuqi, WANG Sicong, LU Liqing, ZHANG Chunfang, CHENG Yuanda 10true ［Abstract］　Objective　To study the differences in serum 25-Hydroxyvitamin D［25(OH)D］ and heat shock protein 70(HSP70) levels among female infertility patients with different types of polycystic ovary syndrome(PCOS). Methods　A total of 250 female infertility patients who visited the doctors at Department of Reproductive Medicine, the First Affiliated Hospital of Dali University from January 2022 to December 2024 were selected as the research subjects. Among the 250 patients, there were 50 patients with tubal infertility(control group) and 200 patients with infertility due to PCOS(PCOS group). According to the Rotterdam diagnostic and typing criteria for PCOS, the infertile patients with PCOS were classified into PCOS-A type(n=50), PCOS-B type(n=50), PCOS-C type(n=50) and PCOS-D type(n=50). According to different serum 25(OH)D levels, the vitamin D levels of the patients were classified as deficiency(<20 ng/mL), insufficiency(20-30 ng/mL), and sufficiency(>30 ng/mL). The levels of serum basic sex hormones, 25(OH)D and HSP70 were compared among the groups. Results　The levels of 25(OH)D and follicle-stimulating hormone(FSH) in the PCOS group were lower than those in the control group, and the body mass index(BMI) as well as the levels of HSP70, luteinising hormone(LH), testosterone(T) and anti-Müllerian hormone(AMH) in the PCOS group were higher than those in the control group, with statistically significant differences between the two groups(P<0.05). There was a statistically significant difference in the distribution of 25(OH)D levels between the two groups(P<0.05). The rate of vitamin D deficiency in the PCOS group was higher. There were no statistically significant differences in the distributions of 25(OH)D levels among the patients with different types of PCOS(P>0.05). There were statistically significant differences in the levels of LH, FSH, T, AMH and HSP70 among the infertile patients with different types of PCOS(P<0.05). The LH level of the patients with PCOS-A type was significantly higher than that of the patients with PCOS-B type, PCOS-C type and PCOS-D type(P<0.05), while the FSH level of the patients with PCOS-A type was significantly lower than that of the patients with PCOS-C type(P<0.05). The HSP70 levels of the patients with PCOS-A type and PCOS-B type were significantly higher than those of the patients with PCOS-D type(P<0.05). Conclusion　Compared with those in the tubal infertility patients, the levels of serum HSP70, LH, T and AMH in the infertile patients with PCOS are significantly increased, while the levels of 25(OH)D and FSH are significantly decreased in the infertile patients with PCOS. The increase of HSP70 in the infertile patients with PCOS shows significant phenotypic differences, with significantly higher levels in the infertile patients with typical hyperandrogenic phenotypes of PCOS(including PCOS-A type and PCOS-B type) compared with those with non-hyperandrogenic phenotypes of PCOS(PCOS-D type). There are no significant differences in the distributions of vitamin D deficiency among the patients with four phenotypes of PCOS. High expression of HSP70 is associated with hyperandrogenemia features, whereas vitamin D deficiency is a common metabolic alteration in the infertile patients with PCOS patients and is unrelated to specific clinical phenotypes.]]> 2026/1/31 11:24:05 1, ZHU Li¹, QIU Yunlin¹, TONG Yinyin¹, WANG Shilian², ZHANG Ruopeng^3,4]]> XU Anli¹, ZHU Li¹, QIU Yunlin¹, TONG Yinyin¹, WANG Shilian², ZHANG Ruopeng^3,4 9true ［Abstract］　Objective　To analyze the levels and clinical significance of D-dimer(D-D), 25-hydroxyvitamin D［25(OH)D］, lymphocyte-to-monocyte ratio(LMR) and interleukin-6(IL-6) in patients with sepsis. Methods　Fifty-five patients with sepsis who were admitted to the 73th Group Military Hospital of People′s Liberation Army from June 2022 to June 2024 were selected as observation group. Other 50 healthy individuals who underwent physical examination during the same period were selected as control group. The levels of plasma D-D, serum 25(OH)D and serum IL-6 were detected for the patients at admission or for the control group during physical examination and LMR was calculated. According to the survival status of the patients within 3 months after admission, they were divided into death group(15 patients) and survival group(40 patients). Multivariate logistic regression was used to analyze the factors influencing the occurrence of death in the sepsis patients. The efficacy of D-D, 25(OH)D, LMR and IL-6 for predicting mortality in the sepsis patients was analyzed by using receiver operating characteristic(ROC) curve. Results　The D-D and IL-6 levels in the observation group were higher than those in the control group, while the 25(OH)D level and LMR in the observation group were lower than those in the control group, with statistically significant differences between the two groups(P<0.05). The Sequential Organ Failure Assessment(SOFA) scores, D-D level and IL-6 level in the death group were higher than those in the survival group, while the 25(OH)D level and LMR in the death group were lower than those in the survival group, with statistically significant differences between the two groups(P<0.05). Higher SOFA scores, D-D level and IL-6 level were risk factors for death in the sepsis patients, while higher 25(OH)D level and LMR were protective factors for survival in the sepsis patients(P<0.05). The efficacy of the combined detection of D-D, 25(OH)D, LMR and IL-6 for predicting mortality in the sepsis patients［AUC(95%CI)=0.834(0.759-0.909)］ was superior to that of the detection of a single indicator(P<0.05), and the sensitivity and specificity were 93.33% and 87.50%, respectively. Conclusion　The levels of plasma D-D and serum IL-6 in sepsis patients increase, while the levels of serum 25(OH)D and LMR in them decrease. The combined detection of these four indicators can effectively predict the prognosis of the sepsis patients.]]> 2026/1/31 0:00:00 1, LIU Shuangqing², ZHANG Ling¹, FANG Weihua¹, YU Shengyang¹, XIE Tao¹, HUANG Qiuli¹, LIU Xiaoyi¹]]> YANG Wei¹, LIU Shuangqing², ZHANG Ling¹, FANG Weihua¹, YU Shengyang¹, XIE Tao¹, HUANG Qiuli¹, LIU Xiaoyi¹ 8true ［Abstract］　Objective　To investigate the association of serum D-dimer(D-D), B cell activating factor(BAFF) and estradiol(E₂) levels with outcomes of frozen-thawed embryo transfer(FET) in patients with endometriosis(EMs) complicated with infertility. Methods　A total of 116 patients with EMs complicated with infertility who were admitted to Department of Reproductive Genetics, Tangshan Maternal and Children Health Hospital from August 2022 to August 2024 were recruited. The patients were divided into pregnancy group(n=64) and non-pregnancy group(n=52) according to their FET outcomes. The general data and the levels of serum D-D, BAFF and E₂ on the first day before performing FET were compared between the two groups. Multivariate logistic regression was used to analyze the factors influencing the FET outcomes of the patients with EMs complicated with infertility. The efficacy of serum D-D, BAFF and E₂ levels in predicting failure to conceive in the patients with EMs complicated with infertility after receiving FET was analyzed by using receiver operating characteristic(ROC) curve. Results　The levels of serum D-D, BAFF and E₂ in the non-pregnancy group were significantly higher than those in the pregnancy group(P<0.05). The results of multivariate logistic regression analysis showed that higher levels of serum D-D［OR(95%CI)=1.346(1.056-1.716)］, BAFF［OR(95%CI)=1.439(1.027-2.016)］ and E₂［OR(95%CI)=1.652(1.121-2.435)］ were independent risk factors for failure to conceive in the patients with EMs complicated with infertility after receiving FET(P<0.05). The results of ROC curve analysis showed that the levels of serum D-D, BAFF and E₂ could effectively predict failure to conceive in the patients with EMs complicated with infertility after receiving FET(P<0.05), and the predictive efficacy of the combination of the three indicators was significantly better than that of a single indicator(Z_{combination of the three indicators-D-D}=2.875, P=0.004; Z_{combination of the three indicators-BAFF}=3.205, P=0.001; Z_{combination of the three indicators-E2}=3.791, P<0.001). Conclusion　The elevated levels of serum D-D, BAFF and E₂ are risk factors for FET failure in the patients with EMs complicated with infertility. The combined detection of the three indicators is helpful for the early identification of high-risk patients and active intervention, thereby improving the success rate of FET.]]> 2026/1/31 11:24:05 FENG Chengwen, FAN Yanling, LIU Baolian 7true ［Abstract］　Objective　To observe the application effect of modified ultrasound-guided fine-needle aspiration cytology(US-FNAC) in examination of thyroid nodules. Methods　A total of 330 patients with thyroid nodules who received US-FNAC in General Hospital of Central Theater Command from March 2023 to July 2024 were recruited. The patients were divided into control group(treated with conventional US-FNAC) and observation group(treated with modified US-FNAC) by random number table method, with 165 patients in each group. In the control group, 3 patients were excluded subsequently due to the previous history of partial thyroidectomy, and 2 patients were excluded after enrollment because they were diagnosed with nasopharyngeal carcinoma and gastrointestinal stromal tumor. Ultimately, 160 patients were included in the control group and 165 patients in the observation group. The control group was treated with non-aspiration conventional US-FNAC using a single-needle, intranodular multi-point puncture technique. The puncture method of the observation group was modified as follows on the basis of that of the control group: (1) sequential punctures using a double-needle technique; (2)fine-needle intranodular multi-site rotating and lifting; (3)syringe negative pressure aspiration. The specimen satisfaction rate of cytological examination, pain condition, and incidence of examination-related adverse reactions were compared between the two groups. Results　The specimen satisfaction rate of cytological examination in the observation group was significantly higher than that in the control group(92.12% vs 81.88%, χ²=7.578, P=0.006). The results of subgroup analysis showed that the specimen satisfaction rates of cytological examination in the observation group were significantly higher than those in the control group in the following stratifications: age <60 years, nodules located in the lower pole, maximum nodule diameter <5 mm, maximum nodule diameter ≥10 mm, nodules with microcalcifications and nodules with macrocalcifications, with statistically significant differences(P<0.05). The pain condition in the control group was milder than that in the observation group, with statistically significant difference(P<0.05). There were no statistically significant differences in the incidence rates of hypertension, tachycardia and puncture site bleeding between the two groups after the examination(P>0.05). Conclusion　The modified US-FNAC technique can significantly increase the specimen satisfaction rate for cytological examination of thyroid nodules, and has good clinical application value.]]> 2026/1/31 11:24:05 1, ZHANG Yu², ZHENG Qiaojun³, YANG Sihan², YUE Ling²]]> ZHANG Junjie¹, ZHANG Yu², ZHENG Qiaojun³, YANG Sihan², YUE Ling² 6true ［Abstract］　Objective　To observe the adverse reactions of high-dose methotrexate(HD-MTX) in treatment of childhood acute lymphoblastic leukemia(ALL), and to analyze the influencing factors of delayed methotrexate(MTX) excretion. Methods　A retrospective analysis was conducted on the clinical data of 74 pediatric patients with ALL who were admitted to Department of Pediatrics, the People′s Hospital of Guangxi Zhuang Autonomous Region from January 2017 to December 2020. HD-MTX-based chemotherapy was performed on 321 cases according to Chinese Children′s Cancer Group ALL-2015(CCCG-ALL-2015) protocol. The blood drug concentration of MTX greater than 1.0 μmol/L after 44 hours of HD-MTX treatment, or that of MTX greater than 0.3 μmol/L after 68 hours of HD-MTX treatment was defined as delayed MTX excretion. The adverse events were graded according to National Cancer Institute-Common Terminology Criteria for Adverse Events version 5.0(NCI-CTCAE v5.0). The grades of adverse events ≥grade Ⅱ were defined as HD-MTX chemotherapy-related and clinically significant adverse reactions. The correlation between the occurrence of adverse reactions and risk stratification as well as immunophenotypes was analyzed. The factors influencing the occurrence of delayed excretion of MTX at 44 hours and 68 hours after HD-MTX treatment were analyzed. Results　Among the 74 pediatric patients with ALL, 40 patients were male(54.05%) and 34 patients were female(45.95%), aged from 1 to 14 years old, with a median age of 4 years old. Risk stratification was low-risk in 73 cases(22.74%), intermediate-risk in 217 cases(67.60%), and high-risk in 31 cases(9.66%). The immunophenotyping results revealed B lineage in 304 cases(94.70%) and T lineage in 17 cases(5.30%). Fusion gene types were BCR/ABL1 in 34 cases(10.59%), ETV6/RUNX1 in 42 cases(13.08%), other types in 13 cases(4.05%), and fusion gene was negative in 232 cases(72.27%). Myelosuppression(62.62%) was the most common in adverse reactions of grades Ⅱ-Ⅳ in the ALL pediatric patients after HD-MTX chemotherapy, followed by gastrointestinal reactions(including diarrhea and constipation, 31.78%) and oral mucositis(30.53%). There was no statistically significant difference in the occurrence of adverse reactions after HD-MTX treatment between the pediatric patients with B lineage ALL and those with T lineage ALL(P>0.05). There were no statistically significant differences in the incidence rates of myelosuppression, infection, oral mucositis and gastrointestinal reaction among the ALL pediatric patients with different risk stratifications after HD-MTX treatment(P>0.05). The incidence of liver damage in the pediatric patients with low-risk ALL was significantly lower than that in the pediatric patients with intermediate-risk ALL(P<0.05), while there was no statistically significant difference in the incidence of liver damage between the pediatric patients with intermediate-risk ALL and those with high-risk ALL(P>0.05). The results of multivariate logistic regression analysis indicated that age >7-14 years［OR(95%CI)= 13.022(1.396-121.436), P=0.024］ and fusion gene type of ETV6/RUNX1［OR(95%CI)=5.863(1.641-20.948), P=0.006］ were independent risk factors for promoting delayed MTX excretion at 44 hours after HD-MTX treatment. Conclusion　The main adverse reactions of HD-MTX in treatment of childhood ALL are myelosuppression, gastrointestinal reaction and oral mucositis. Age >7-14 years and fusion gene type of ETV6/RUNX1 are independent risk factors for promoting delayed MTX excretion at 44 hours after HD-MTX treatment.]]> 2026/1/31 0:00:00 1,2, DAI Yan², LI Xinye², TANG Huihe², MA Hongyan², HUANG Jinxian², CHEN Zhaohui², LI Junjun^1,2]]> LI Qin^1,2, DAI Yan², LI Xinye², TANG Huihe², MA Hongyan², HUANG Jinxian², CHEN Zhaohui², LI Junjun^1,2 5true ［Abstract］　Objective　To analyze the clinical efficacy of parent-oriented group and individual cognitive-behavioral therapy(CBT) combined with family-based early intervention training in children with autism spectrum disorder(ASD). Methods　A total of 100 pediatric patients with ASD who were admitted to Xuzhou Children′s Hospital Affiliated to Xuzhou Medical University from January 2024 to February 2025 were recruited. The pediatric patients were divided into group-CBT group(receiving group CBT combined with family-based early intervention training, 34 patients), individual-CBT group(receiving individual CBT combined with family-based early intervention training, 33 patients) and control group(receiving family-based early intervention training, 33 patients) by random number table method. Family-based early intervention training was conducted once weekly, with a training period of 3 months. CBT was conducted once weekly, for a total of 8 times. Gesell Developmental Schedules(GDS) scores, Autism Behavior Checklist(ABC) scores and Autism Treatment Evaluation Checklist(ATEC) scores were compared among the pediatric patients in the three groups before and after the intervention. Self-Rating Anxiety Scale(SAS) scores and Tool to Measure Parenting Self-Efficacy(TOPSE) scores were compared among the pediatric patients′ parents in the three groups before and after the intervention. Results　After intervention, the scores of adaptability, fine motor skills, language and personal social domains in the GDS of the pediatric patients in the three groups were significantly higher than those before intervention(P<0.05), and the scores of adaptability, language and personal social domains in the group-CBT group were higher than those in the individual-CBT group and the control group, with statistically significant differences(P<0.05). After intervention, the scores of each dimension of ATEC in the pediatric patients in the three groups were significantly lower than those before intervention(P<0.05), and the scores of language, social interaction and behavior dimensions in the group-CBT group were lower than those in the individual-CBT group and the control group, with statistically significant differences(P<0.05). After intervention, the scores of ABC in the pediatric patients in the three groups were significantly lower than those before intervention(P<0.05), and the scores of ABC in the group-CBT group were lower than those in the individual-CBT group and the control group, with statistically significant differences(P<0.05). After intervention, the scores of SAS in the pediatric patients′ parents in the group-CBT group and the individual-CBT group were significantly lower than those before intervention(P<0.05), while the scores of SAS in the control group showed no significant changes before and after intervention(P>0.05). After intervention, the scores of SAS in the group-CBT group were lower than those in the individual-CBT group and the control group, with statistically significant differences(P<0.05). After intervention, the scores of each dimension and the total scores of TOPSE in the pediatric patients′ parents in the group-CBT group were higher than those in the individual-CBT group and the control group, with statistically significant differences(P<0.05). Conclusion　CBT intervention for parents of pediatric patients with ASD can improve their mental health status, enhance their self-efficacy in parenting, thereby promoting the behavioral development of the pediatric patients with ASD. Moreover, the intervention effect of group CBT is better than that of individual CBT.]]> 2026/1/31 11:24:05 MENG Lulu, BAO Kexiu, QIU Aizhen, WANG Min, WANG Yan, LI Jie, WANG Ya 4true ［Abstract］　Objective　To explore the application effect of different methods of modified Nuss bar fixation for non-thoracoscopic-assisted pectus excavatum(PE) surgery. Methods　A retrospective analysis was conducted on the clinical data of 53 patients who underwent PE surgery by using modified Nuss bar in Department of Thoracic Surgery, Xiangya Hospital of Central South University from July 2021 to December 2023. According to whether or not steel wire fixation was used and the fixation methods, the patients were divided into non-steel wire fixation group(11 patients), unilateral fixation group(15 patients), and bilateral fixation group(21 patients), excluding 5 patients with 2 steel bars fixed and 1 patient with steel wire bridge. The overall condition of the patients was analyzed and the clinical data were compared among the three groups. Results　Among the 53 patients, 45 patients were male and 8 patients were female, with a median age of 15 years and a median Haller index of 3.86. Steel wire fixation was not used in 11 patients(20.75%), and unilateral steel wire fixation was used in 15 patients(28.30%), and bilateral steel wire fixation in 27 patients(50.94%). The median follow-up time was 17 months. Fifty-one patients had a follow-up period of 1 year, and 15 patients had a follow-up period of 2 years. The incidence of postoperative complications was 28.30%, and the proportions of the patients who were satisfied with the orthopedic treatment outcomes at 1 month, 1 year and 2 years after surgery were 92.45%, 90.20% and 80.00%, respectively. There were statistically significant differences in the preoperative Haller index and operation duration among the three groups(P<0.05), and the operation duration of the non-steel wire fixation group was significantly shorter than that of the bilateral fixation group(P<0.05). There were no statistically significant differences in the postoperative pain scores, Haller indexes at 1 month and 1 year after surgery, length of hospital stay after surgery, postoperative complications and the proportion of the patients who were satisfied with the orthopedic treatment outcomes at 1 year after surgery among the three groups(P>0.05). Conclusion　The modified Nuss bar fixation has a certain orthopedic effect. The non-wire fixation method is potentially feasible for non-thoracoscopic-assisted PE surgery.]]> 2026/1/31 11:24:05 AI Yan, LI Linfeng, LI Xizhe, WANG Sicong, YANG Yuqi, CHENG Yuanda 3true ［Abstract］　Objective　To analyze the correlation between plasma polymyxin B concentration and risk of acute kidney injury(AKI). Methods　A retrospective analysis was conducted on the clinical data of 53 patients who were hospitalized and treated with polymyxin B in the People′s Hospital of Guangxi Zhuang Autonomous Region from April 1 2024 to August 8, 2024. After 3 doses of polymyxin B were administered for the patients to reach steady state, 2-3 mL of their venous blood was collected 30 minutes before the next dose was administered. A mass spectrometer YS EXACT 9900MD was used to detect plasma polymyxin B concentration. The effect of polymyxin B combined with nephrotoxic antibiotics on the serum creatinine levels of the patients was analyzed. The effect of the use of polymyxin B on the renal function indicators of the patients was analyzed. Multivariate logistic regression was used to analyze the influencing factors for the occurrence of AKI after the administration of polymyxin B. Results　Compared with the use of polymyxin B in no combination with nephrotoxic antibiotics, the combination of polymyxin B and nephrotoxic antibiotics did not significantly affect the serum creatinine levels of the patients(P>0.05). Compared with those of the patients before the administration of polymyxin B, the serum creatinine and urea levels of the patients increased, while the creatinine clearance rate decreased after the administration of polymyxin B, and the differences were statistically significant(P<0.05). The results of multivariate logistic regression analysis showed that a higher level of plasma polymyxin B concentration was an independent risk factor for the occurrence of AKI after the administration of polymyxin B［OR(95%CI)=1.874(1.220-2.879), P=0.004］. The results of receiver operating characteristic(ROC) curve analysis showed that plasma polymyxin B concentration could effectively predict the occurrence of AKI ［AUC(95%CI)=0.734(0.599-0.868), P=0.004］, with an optimal cut-off value of 3.18 mg/L, a corresponding sensitivity of 65.4%, and a specificity of 77.8%. Conclusion　After receiving polymyxin B treatment, the patients′ serum creatinine and urea levels increase. The occurrence of AKI is closely related to plasma polymyxin B concentration. Clinicians should conduct monitoring of plasma polymyxin B concentration during the treatment process to precisely adjust the medication regimen.]]> 2026/1/31 11:24:05 1, WEI Ruhua², CHEN Xiaoyu¹, LI Yan¹, ZHANG Li¹, WEI Shuilin¹, TAN Sitao¹, GUO Qiuyan¹]]> LI Boren¹, WEI Ruhua², CHEN Xiaoyu¹, LI Yan¹, ZHANG Li¹, WEI Shuilin¹, TAN Sitao¹, GUO Qiuyan¹ 2true ［Abstract］　Objective　To explore the protective mechanism of baicalein(BAL) on cardiomyocyte injury caused by iron overload. Methods　An iron-overload cell model was constructed by intervening cardiomyocyte H9c2 with 200 μmol/L ferric citrate(FC) for 24 hours. The protective effects of different concentrations of BAL(3, 10, and 30 μmol/L) on cardiomyocytes with iron overload were analyzed. The protective effects of BAL on cardiomyocytes by inhibiting the Kelch-like ECH-associated protein 1(Keap1)/ nuclear factor erythroid 2-related factor 2(Nrf2)/ heme oxygenase-1(HO-1) pathway(treated with HO-1 inhibitor SnPP or Nrf2 inhibitor ML385) were analyzed. Cell viability was detected by using the CCK-8 assay. The expression levels of inflammatory factors including tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β) and interleukin-6(IL-6) were detected by using real-time quantitative polymerase chain reaction(RT-qPCR). The level of reactive oxygen species(ROS) was detected by using 2′,7′-dichlorodihydrofluorescein diacetate(DCFH-DA) fluorescent probe. The expression of 4-hydroxynonenal(4-HNE) was detected by using immunofluorescence staining. The expression levels of oxidative stress indicators of malondialdehyde(MDA) and glutathione(GSH), and iron content were detected by using reagent kits. The expression levels of proteins related to the Keap1/Nrf2/HO-1 pathway and ferroptosis were detected by using Western blot assay. Results　BAL could enhance the viability of iron-overloaded H9c2 cells, reduce the expression levels of inflammatory factors of TNF-α, IL-1β and IL-6, as well as oxidative stress indicators of ROS and 4-HNE, making the expression level of MDA decrease and the expression level of GSH increase, and the intervention effects were dose-dependent. In addition, after intervention with BAL, iron deposition in the iron-overloaded H9c2 cells decreased, while the expressions of ferroptosia-related protein solute carrier family 7 member 11(SLC7A11), glutathione peroxidase 4(GPx4), and solute carrier family 3 member 2(SLC3A2) protein increased, and the expression of cyclooxygenase-2(COX-2) protein decreased. The results of Western blot assay showed that BAL activated the Keap1/Nrf2/HO-1 pathway, inhibited the expression of Keap1 protein, and promoted the expressions of Nrf2 and HO-1 proteins, and the intervention effects were dose-dependent. After treatment with SnPP or ML385, the protective effect of BAL on iron-overloaded H9c2 cells weakened, and the cell viability decreased, and the expressions of inflammatory factors increased, and the oxidative stress and lipid peroxidation enhanced, and the iron deposition increased, and the ferroptosis intensified. Conclusion　BAL may mitigate iron overload-induced cardiomyocyte injury via activating the Keap1/Nrf2/HO-1 pathway.]]> 2026/1/31 11:24:05 LONG Xingjiang, QIN Jiaofeng, HUANG Xiangui. 1true