［Abstract］　Objective　To study the role and mechanism of calcium-sensing receptor(CaSR) in pulmonary venous remodeling in pulmonary hypertension(PH) mice. Methods　A total of 28 C57/BL6 mice were randomly divided into normoxia(Nor) group, normoxia+NPS2143(Nor+NPS) group, hypoxia combined with SU5416(HySu) group and hypoxia combined with SU5416+NPS2143(HySu+NPS) group, with 7 mice in each group. A PH mouse model was developed by feeding under hypoxic(10% O₂) condition and injecting SU5416 subcutaneously. PH was assessed by using hemodynamic measurement and right ventricular hypertrophy index(RVHI) analysis, and vascular remodeling was assessed by using Weigert′s elastin staining, hematoxylin and eosin(HE) staining, alpha-smooth muscle actin(α-SMA) immunohistochemical staining, and CaSR immunohistochemical staining. The expression levels of CaSR, canonical transient receptor potential protein(TRPC) 6 and TRPC4 in pulmonary vein were detected by Western blot. Results　The PH mouse model was successfully developed by hypoxia combined with SU5416. The right ventricular systolic pressure(RVSP) and RVHI of mice in the HySu group were significantly higher than those in the Nor group. The distal pulmonary veins of PH mice underwent vascular remodeling, while the proximal pulmonary veins of PH mice showed no vascular remodeling. The expression levels of CaSR and TRPC6 in the PH mice were significantly up-regulated compared with those in the mice of the Nor group(P<0.05), but there was no significant difference in TRPC4 expression between the PH mice and the mice of the Nor group(P>0.05). After the intervention with NPS2143, the RVSP and RVHI of mice in the HySu+NPS group were significantly lower than those in the HySu group(P<0.05), and NPS2143 could significantly alleviate the distal pulmonary venous remodeling induced by hypoxia combined with SU5416, and down-regulated the expressions of CaSR and TRPC6 in the distal pulmonary veins of PH mice(P<0.05). Conclusion　CaSR plays a core role in the remodeling of distal pulmonary veins in PH mice. Hypoxia combined with SU5416 may lead to the upregulation of TRPC6 expression by upregulating the expression of CaSR in the smooth muscle of distal pulmonary veins, leading to the pulmonary venous remodeling.]]> 2025/2/7 8:31:46 1,2, XUE Yan^1,2, MO Qiudi^1,3, YUAN Hong¹, PENG Gongyong¹]]> GAO Xiaohua^1,2, XUE Yan^1,2, MO Qiudi^1,3, YUAN Hong¹, PENG Gongyong¹ 4true ［Abstract］　Objective　To explore the diagnostic value of the combined detection of fractional exhaled nitric oxide(FeNO) and small airway function indicators for cough-variant asthma(CVA). Methods　A total of 140 patients with chronic cough who visited the outpatient Department of Respiratory and Critical Care Medicine of the People′s Hospital of Guangxi Zhuang Autonomous Region from March 2023 to February 2024 were selected as the study subjects. According to the patients′ diagnostic results, they were divided into CVA group(43 cases) and non-CVA group(97 cases). The baseline data, FeNO levels, and small airway function were compared between the two groups, and the diagnostic efficacy of the combined detection of FeNO and small airway function indicators for CVA were analyzed. Results　The detected FeNO at a 50 mL/s flow rate(FeNO₅₀)(53.00 ppb vs 19.00 ppb), the detected FeNO at a 200 mL/s flow rate(FeNO₂₀₀)(19.00 ppb vs 8.00 ppb) and the level of alveolar nitric oxide(CaNO)(5.20 ppb vs 3.30 ppb) in the CVA group were higher than those in the non-CVA group, and the differences were statistically significant(P<0.05). In the CVA group, the FeNO₅₀ levels in the patients complicated with small airway dysfunction were significantly higher than those in the patients without small airway dysfunction(64.50 ppb vs 26.00 ppb, P<0.05). The results of receiver operating characteristic(ROC) curve analysis showed that FeNO combined with small airway function indicators, maximal mid-expiratory flow(MMEF) and maximum expiratory flow at 25% of vital capacity(MEF_25%) had certain diagnostic value for CVA(P<0.05), and FeNO₅₀ combined with MMEF had better diagnostic value for CVA［area under the curve(AUC)(95%CI)=0.79(0.70, 0.87)］. Conclusion　The levels of FeNO in CVA patients are significantly higher than those in non-CVA patients, and the combined detection of FeNO and small airway function indicators has relatively high diagnostic value for CVA.]]> 2025/2/7 0:00:00 LU Jinhua, CHEN Ru, LU Jingchan, LU Ailing, LIAO Xiaoling, HU Guoping, HUANG Luying 3true ［Abstract］　Stromal interaction molecule 1(STIM1), a single transmembrane protein located in the endoplasmic reticulum, dynamically senses the changes in intracellular calcium concentration and then transmits calcium signals to ORAI calcium release-activated calcium modulator 1(ORAI1) on the cell membrane, inducing downstream calcium signaling and mediating cellular calcium influx, thereby participating in various physiological functions such as cell proliferation and migration, immune cell activation, gene transcription, etc. The STIM1/ORAI1 signaling pathway plays a key regulatory role in a variety of respiratory diseases, including pulmonary hypertension, bronchial asthma, chronic obstructive pulmonary disease, acute lung injury/acute respiratory distress syndrome, and idiopathic pulmonary fibrosis. In this paper, the latest research progress on the role and mechanism of the STIM1/OAI1 signaling pathway in respiratory diseases is reviewed, with the aim of providing more ideas for the studies on the related diseases and finding possible new research targets for effective clinical diagnosis and treatment.]]> 2025/2/7 8:31:46 LIANG Jieping, PENG Gongyong 2true ［Abstract］　Chronic obstructive pulmonary disease(COPD) is a common chronic respiratory disease characterized by persistent respiratory symptoms and airflow limitation, with high morbidity, disability and mortality. According to the World Health Organization′s prediction, COPD will become the third leading chronic diease causing deaths around the world by 2030, imposing substantial economic burden. Due to the insidious onset of early COPD, insufficient understanding of clinical symptoms such as cough and sputum in some COPD patients, and low popularity rate of pulmonary function test, the proportion of early clinical diagnosis and effective treatment of COPD is extremely low, which delays the optimal time for diagnosis and treatment of COPD. Early diagnosis and treatment can delay the progression of pulmonary function decline in COPD patients, reduce the times of acute exacerbation and the hospitalization rate, and improve the quality of life in early COPD patients. Therefore, it is necessary to strengthen the early screening of COPD, so as to achieve early diagnosis, early intervention and early treatment.In this paper, the relevant literature at home and abroad in recent years is summarized, and the research progress of early screening methods for COPD is reviewed.]]> 2025/2/7 8:31:46 LI Jing, JIANG Yongliang 1true