［Abstract］　Objective　To explore the differences in effects of progesterone receptor membrane component 1(PGRMC1) in a nude mouse model with human breast cancer xenografts on the proliferation of breast cancer xenografts under the stimulation of progesterone and norethisterone(NET). Methods　A human breast cancer cell line(MCF7) with stable expression of PGRMC1 was constructed. Thirty-six female BALB/c-nu nude mice were divided into MCF7/HA-PGRMC1 group(n=18) and MCF7/HA-Vector group(n=18) according to random number table method. Estradiol(E₂) sustained-release tablets were subcutaneously embedded in the neck of each mouse. After subcutaneous embedding of E₂ sustained-release tablets for 24 hours, MCF7/HA-PGRMC1 and MCF7/HA-Vector cell suspensions were respectively injected into the subcutaneous tissues on the left and right sides of the nude mice in the two groups at a dose of 150 μL, with a cell count of 1×10⁷. When tumors grew to about 100 mm³ in size, the nude mice in the two groups were further divided into three subgroups: the E₂+placebo subgroup(receiving subcutaneous embedding of E₂ plus placebo)(n=6), the E₂+progesterone subgroup(receiving subcutaneous embedding of E₂ plus progesterone sustained-release tablets)(n=6) and the E₂+NET subgroup(receiving subcutaneous embedding of E₂ plus NET sustained-release tablets)(n=6). The volume changes of the tumors were recorded and tumor growth curves were plotted. After 45 days, the tumor tissues were completely excised, and immunohistochemical staining was performed to detect PGRMC1 and Ki67 expressions in the xenograft tissues. Results　The MCF7/HA-PGRMC1 strain was successfully constructed. In the MCF7/HA-PGRMC1 group, there was no statistically significant difference in tumor growth between the E₂+progesterone subgroup and the E₂+placebo subgroup(P>0.05), while the E₂+NET subgroup showed significant tumor growth compared with the E₂+placebo subgroup(P<0.05). In the MCF7/HA-Vector group, there was no statistically significant difference in tumor growth between the E₂+NET subgroup and the E₂+placebo subgroup(P>0.05) as well as between the E₂+progesterone subgroup and the E₂+placebo subgroup(P>0.05). In the MCF7/HA-PGRMC1 group, Ki67 showed strong positive expression in the E₂+NET subgroup, and PGRMC1 was positively correlated with Ki67 expression(r=0.892, P=0.014). Conclusion　This experiment confirms that overexpression of PGRMC1 can specifically mediate the proliferation-promoting effect of NET combined with E₂ on breast cancer in nude mice in vivo, while the combination with progesterone does not promote the additional proliferation. This discovery provides crucial animal experimental evidence for the selection of progesterone types in clinical hormone therapy.]]> 2025/11/29 0:00:00 1, JU Rui², XIE Dan¹, ZHANG Zheng¹, ZHU Jianguo¹, ZHANG E¹, LI Li³]]> ZHAO Yue¹, JU Rui², XIE Dan¹, ZHANG Zheng¹, ZHU Jianguo¹, ZHANG E¹, LI Li³ 5true ［Abstract］　Objective　To observe the efficacy of the “peach-like” procedure combined with levonorgestrel-releasing intrauterine system(LNG-IUS) in treatment of adenomyosis. Methods　A retrospective analysis was conducted on the clinical data of 60 patients with adenomyosis who were admitted to Henan Provincial People′s Hospital from January 2023 to June 2024. Thirty patients who underwent the “peach-like” procedure combined with LNG-IUS for adenomyosis were selected as the observation group, and other 30 patients who underwent laparoscopic uterine lesion resection for adenomyosis were selected as the control group. The clinical therapeutic effects were compared between the two groups 3 months after the operation. Results　Before the operation, there were no statistically significant differences in uterine volume, Visual Analogue Scale(VAS) scores and Pictorial Blood Loss Assessment Chart(PBAC) scores between the two groups(P>0.05). Compared with those before the operation, the uterine volume, VAS scores and PBAC scores were decreased in the two groups 3 months after the operation, with statistically significant differences(P<0.05). At 3 months after the operation, the uterine volume in the observation group was smaller than that in the control group, and the VAS scores and PBAC scores in the observation group were lower than those in the control group, with statistically significant differences between the two groups(P<0.05). The duration of operation in the observation group was shorter than that in the control group, and the intraoperative blood loss in the observation group was less than that in the control group, with statistically significant differences between the two groups(P<0.05). There was no statistically significant difference in the length of hospital stay between the two groups(P>0.05). Conclusion　Compared with laparoscopic uterine lesion resection, the “peach-like” procedure combined with LNG-IUS in treatment of adenomyosis can shorten surgical time, reduce blood loss, decrease postoperative uterine volume, increase menstrual blood volume and improve dysmenorrhea symptoms more significantly. The combination treatment is worthy of clinical recommendation.]]> 2025/11/29 0:00:00 1, LUO Suiyu², GANG Xiaoqing², LI Xin¹, XU Shuo¹]]> YAN Zhenzhen¹, LUO Suiyu², GANG Xiaoqing², LI Xin¹, XU Shuo¹ 4true ［Abstract］　Objective　To explore the feasibility and clinical efficacy of a novel hysteroscopic levonorgestrel-releasing intrauterine system(LNG-IUS) non-suture fixation in treatment of adenomyosis. Methods　Forty-four patients with adenomyosis who were admitted to Beijing Tiantan Hospital, Capital Medical University from January 2024 to March 2025 and were treated with hysteroscopic LNG-IUS non-suture fixation for adenomyosis were selected. The patients′ general data, clinical manifestations, operative duration, operative complications, postoperative LNG-IUS expulsion and other follow-up data were collected. The operation effects were assessed by using a one way ANOVA paired T test. Results　A total of 44 patients were included, with average age of (40.16±4.54)years, average number of pregnancy of (2.30±1.62), average number of delivery of (1.14±0.80), average uterine cavity depth of (10.58±1.03)cm, average uterine volume of 130.31(90.60, 174.04)mL, average operative duration of (14.54±5.01)min, and intraoperative blood loss of (4.59±3.34)mL. Among them, there were 36 cases(81.82%) of dysmenorrhea, 44 cases(100.00%) of menorrhagia, 23 cases(52.27%) of anemia, 17 cases(38.64%) with a history of LNG-IUS expulsion, and 7 cases(15.91%) with a history of LNG-IUS dislodgement. Among the 44 patients, 13 patients underwent hysteroscopic electroresection of endometrial polyps simultaneously, and 3 patients underwent electroresection of submucosal myoma of the uterus simultaneously, and 24 patients underwent aspiration of uterine lining to thin the endometrium at the same time. None of the patients presented with complications such as uterine perforation, massive hemorrhage, fluid overload and postoperative infection. The average postoperative follow-up time was (7.01±1.20)months, and no postoperative LNG-IUS expulsion occurred in all the patients. The follow-up results at 6 months after the operation showed that the effective rate of dysmenorrhea was 91.67%(33/36), and the effective rate of menorrhagia was 95.45%(42/44). At 1 month after the operation, the hemoglobin content of the patients was 124.00(114.50, 130.00)g/L, which was significantly increased compared with that before the operation［108.00(96.25, 118.75)g/L］(Z=5.661, P<0.001). At 1 month and 6 months after the operation, the patients′ menstrual Pictorial Blood Loss Assessment Chart(PBLAC) scores, Visual Analogue Scale(VAS) scores for dysmenorrhea, and uterine volume were decreased compared with those before the operation, and the differences were statistically significant(P<0.05). Conclusion　The hysteroscopic LNG-IUS non-suture fixation is a safe and effective technique, especially suitable for adenomyosis patients with a history of LNG-IUS expulsion or a history of LNG-IUS dislodgement. This surgical method is simple to operate, minimally invasive, with a short operation time, less hemorrhage and a quick postoperative recovery in the patients, and thus is worthy of clinical application.]]> 2025/11/29 21:17:25 ZHANG Luping, ZHANG Shengpeng, LI Jinghua, FENG Limin 3true ［Abstract］　Objective　To explore influencing factors of postpartum urinary retention(PUR) in vaginal delivery primiparas without labor analgesia, and to provide evidence-based basis for the formulation of targeted preventive measures in clinical practice. Methods　A total of 105 primiparas who were admitted to Beijing Obstetrics and Gynecology Hospital, Capital Medical University for vaginal delivery without labor analgesia from December 2021 to December 2024 were selected. According to whether the patients developed PUR, they were divided into non-PUR group(70 patients) and PUR group(35 patients). The general data and clinical indicators were compared between the two groups. Multivariate logistic regression was used to analyze the factors affecting the development of PUR and receiver operating characteristic(ROC) curve was used to analyze the efficacy of the research indicators in predicting PUR. Results　There were statistically significant differences between the two groups in terms of postpartum hemorrhage volume, duration of the third stage of labor, classification of gestational weight gain, and midwifery method(P<0.05). The results of multivariate logistic regression analysis indicated that excessive weight gain during pregnancy［OR(95%CI)=5.028(1.500-16.854), P=0.009］, insufficient weight gain during pregnancy［OR(95%CI)=2.892(1.010-8.283), P=0.048］ and large postpartum hemorrhage volume［OR(95%CI)=1.009(1.003-1.015), P=0.002］ were independent risk factors for the development of PUR. The prediction model was constructed based on the above 3 risk factors. The results of ROC curve analysis indicated that the model could effectively predict the development of PUR［ area under the curve(AUC)(95%CI)=0.762(0.663-0.861)］, with a sensitivity of 65.7%, a specificity of 78.6%, and a Youden′s index of 0.443. Conclusion　The risk factors for the development of PUR after vaginal delivery in primiparas without labor analgesia are abnormal gestational weight gain(excessive weight gain or insufficient weight gain) and large postpartum hemorrhage volume. Reasonable management of weight during pregnancy and prevention and control of postpartum hemorrhage should be strengthened in clinical practice to reduce the incidence of PUR.]]> 2025/11/29 21:17:25 1, ZHAO Yue¹, LAN Xueli², JU Rui³]]> ZHANG E¹, ZHAO Yue¹, LAN Xueli², JU Rui³ 2true ［Abstract］　Objective　To explore the effects of different intervention movements on intravaginal pressure in research subjects. Methods　A total of 163 subjects who underwent pelvic floor examination in Gynecological Pelvic Floor Center, Henan Provincial People′s Hospital from June 2024 to August 2024 were selected, among whom 50 subjects who suffered from stress urinary incontinence(SUI) were selected as SUI group and 113 subjects without SUI were selected as non-SUI group. Using body mass index(BMI)≥28 kg/m² as the obesity standard, there were 8 obese individuals(obese group) and 155 non-obese individuals(non-obese group). The changes in intravaginal pressure of the subjects under the conditions of lying on the back at rest, electrical stimulation of abdominal muscles, abdominal crunch movement, forceful coughing and forceful defecation were observed. Results　For the subjects, their intravaginal pressure was the highest when they underwent forceful coughing movement［(111.02±15.37)cmH₂O］, followed by that when they underwent forceful defecation movement ［(97.72±11.35) cmH₂O］, then followed by that when they underwent abdominal crunch movement ［(87.15±10.72)cmH₂O］ and finally followed by that when they underwent electrical stimulation of abdominal muscles［(78.95±7.98)cmH₂O］. There were statistically significant differences between the intravaginal pressure measured under the conditions of different movements mentioned above and the intravaginal pressure measured under the condition of lying on the back at rest ［(75.72±7.85 )cmH₂O］(P<0.001). Under the conditions of lying on the back at rest, electrical stimulation of abdominal muscles, abdominal crunch movement and forceful defecation movement, the intravaginal pressure of the SUI group was significantly higher than that of the non-SUI group(P<0.05). However, there was no statistically significant difference in the intravaginal pressure between the two groups under the condition of forceful coughing movement(P>0.05). Under the conditions of lying on the back at rest, electrical stimulation of abdominal muscles and abdominal crunch movement, the intravaginal pressure of the obese group was significantly higher than that of the non-obese group(P<0.05), and under the conditions of forceful coughing movement and forceful defecation movement, there was no statistically significant difference in the intravaginal pressure between the two groups(P>0.05). Conclusion　Both forceful coughing and forceful defecation can exert considerable pressure on the pelvic floor, so in daily life, it is advisable to avoid forceful coughing and keep a normal bowel movement. Compared with abdominal crunch movement, electrical stimulation of abdominal muscles has a smaller impact on pelvic floor pressure. Therefore, electrical stimulation of abdominal muscles is a better choice for treating diastasis recti abdominis.]]> 2025/11/29 21:17:25 LUO Suiyu, XU Jun, ZHANG Lingyun, LI Yaping, CHEN Mengyao 1true