| 摘要: |
| 【】目的:探讨微小RNA-181a-5p(miR-181a-5p)、微小RNA-132-5p(miR-132-5p)与糖尿病肾病(DN)患者病情及预后的关系。方法:选取2021年4月~2024年4月收治的121例DN患者为DN组,依据1年预后分为预后良好组(96例)和预后不良组(25例)；同期纳入119例单纯糖尿病患者为单纯糖尿病组,同期纳入116例健康体检人群作为对照组。采用实时荧光定量PCR法检测血清miR-181a-5p、miR-132-5p水平,评估DN患者肾脏病理损伤程度,记录尿素氮(BUN)、血尿酸、血肌酐(Scr)等临床资料。采用Spearman法分析相关性,采用多因素logistic回归分析筛选独立影响因素,绘制ROC曲线构建预后不良预测模型,采用DeLong检验比较AUC。结果:与对照组、单纯糖尿病组比较,DN组血清miR-181a-5p、miR-132-5p水平较低(P＜0.05)。随着DN患者肾脏病理损伤程度加重,血清miR-181a-5p、miR-132-5p水平逐渐降低(P＜0.05)。血清miR-181a-5p、miR-132-5p水平与肾小球分级、间质炎症评分、间质性纤维化和小管萎缩(IFTA)评分均呈负相关(P＜0.05)。与预后良好组比较,预后不良组Scr较高,血清miR-181a-5p、miR-132-5p水平较低(P＜0.05)。miR-181a-5p、miR-132-5p是DN患者预后不良的独立保护因素(P＜0.05)。miR-181a-5p、miR-132-5p单独及二者联合预测DN患者预后不良的AUC为0.838、0.821、0.954,其中联合AUC较高(Z=2.278、2.612,P＜0.05)。结论:DN患者血清miR-181a-5p、miR-132-5p显著下降,二者与病情进展及预后密切相关,可联合预测DN预后不良。 |
| 关键词: 糖尿病肾病 微小RNA-181a-5p 微小RNA-132-5p 病情 预后 |
| DOI： |
| 分类号: |
| 基金项目:连云港市科技计划项目(SF2324)；连云港市卫生健康面上科技项目(202115) |
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| The relationship between miR-181a-5p, miR-132-5p with the condition and prognosis of patients with diabetic nephropathy |
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王莉
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Nanjing University of Traditional Chinese Medicine Lianyungang Affiliated Hospital
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| Abstract: |
| Objective: To explore the relationship between microRNA-181a-5p (miR-181a-5p), microRNA-132-5p (miR-132-5p) with the condition and prognosis of patients with diabetic nephropathy (DN). Methods: From April 2021 to April 2024, 121 DN patients were marked as the DN group and assigned into a good prognosis group (96 cases) and a poor prognosis group (25 cases) based on their 1-year prognosis. Meantime, 119 patients with simple diabetes were included in the simple diabetes group, and 116 individuals who underwent health check ups were included as the control group. Real-time fluorescence quantitative PCR was used to detect serum miR-181a-5p and miR-132-5p. The degree of renal pathological damage in DN patients was evaluated, and clinical data such as blood urea nitrogen (BUN), blood uric acid, and blood creatinine (Scr) were recorded. Spearman method was used to analyze correlation. Multivariate logistic regression analysis was used to screen for independent influencing factors. ROC curve was plotted to construct a poor prognosis prediction model. DeLong test was used to compare AUC. Results: Compared with the control group and the simple diabetes group, the serum miR-181a-5p and miR-132-5p in DN group were lower (P<0.05). As the degree of renal pathological damage in DN patients worsened, serum miR-181a-5p and miR-132-5p gradually decreased (P<0.05). Serum miR-181a-5p and miR-132-5p were negatively correlated with glomerular grading, interstitial inflammation score, and the interstitial fibrosis and tubular atrophy (IFTA) score (P<0.05). Compared with the good prognosis group, the poor prognosis group had higher Scr and lower serum miR-181a-5p and miR-132-5p (P<0.05). MiR-181a-5p and miR-132-5p were independent protective factors for poor prognosis in DN patients (P<0.05). The AUC values of miR-181a-5p, miR-132-5p alone, and their combination in predicting poor prognosis in DN patients were 0.838, 0.821, and 0.954, respectively, with the combined AUC being relatively high (Z=2.278, 2.612, P<0.05). Conclusion: Serum miR-181a-5p and miR-132-5p are prominently decreased in DN patients. The two are closely related to disease progression and prognosis, and can jointly predict poor prognosis of DN. |
| Key words: Diabetic nephropathy MicroRNA-181a-5p MicroRNA-132-5p Disease condition Prognosis |
