| 引用本文: | 赵文雅,毕奥贞,赵明,郭峪琳,胡婧雯,尹富强,刘夏.Garcinone C 对鼻咽癌细胞氧化损伤的影响及相关机制研究[J].中国临床新医学,0,():-. |
| zhao wen ya,Bi ao zhen,Zhao ming,Guo yu lin,Hu jing wen,Yin fu qiang.Garcinone C 对鼻咽癌细胞氧化损伤的影响及相关机制研究[J].中国临床新医学,0,():-. |
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| 摘要: |
| 目的 探讨天然产物Garcinone C对鼻咽癌细胞氧化损伤的影响及相关机制。 方法 体外培养鼻咽癌细胞HONE1和HK1，分别给予0 μmol/L、5 μmol/L、7.5 μmol/L的Garcinone C进行药物干预。通过酶标仪检测细胞中脂质过氧化产物丙二醛（Malondialdehyde，MDA）的含量。利用流式细胞术结合BODIPY 581/591 C11探针染色检测细胞内脂质过氧化水平。利用免疫荧光实验检测DNA损伤标志性蛋白 γ-H2A.X的核内表达情况。通过Western Blot检测氧化损伤相关蛋白γ-H2A.X、RAD50、p53、KEAP1的表达水平。结果 经Garcinone C处理后，HONE1和 HK1细胞内MDA含量和脂质过氧化水平显著升高（P ＜0.05），核内γ-H2A.X表达量显著增加（P ＜0.05），γ-H2A.X、p53蛋白表达显著上升（P ＜0.05），KEAP1、RAD50 蛋白表达显著下降（P ＜0.05）。结论 Garcinone C可通过诱导鼻咽癌细胞氧化损伤而发挥抗肿瘤作用，有望成为治疗鼻咽癌的候选先导化合物。 |
| 关键词: Garcinone C 鼻咽癌 氧化损伤 |
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| 基金项目:国家自然科学(82260721,81903644)；广西自然科学基金重点项目(2024GXNSFDA010045)；广西医科大学研究生教育创新计划项目(YCSW2025264) |
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| A Study on the effects of Garcinone C on oxidative damage in nasopharyngeal carcinoma cells and the related mechanisms |
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zhao wen ya, Bi ao zhen, Zhao ming, Guo yu lin, Hu jing wen, Yin fu qiang
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Guangxi Medical University
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| Abstract: |
| Objective To investigate the effects of the natural product Garcinone C on oxidative damage in nasopharyngeal carcinoma cells and the underlying mechanisms. Methods Nasopharyngeal carcinoma cell lines HONE1 and HK1 were cultured in vitro and treated with Garcinone C at concentrations of 0 μmol/L, 5 μmol/L, and 7.5 μmol/L. The content of malondialdehyde (MDA), a lipid peroxidation product, was measured using a microplate reader. Intracellular lipid peroxidation levels were assessed by flow cytometry combined with BODIPY 581/591 C11 probe staining. Immunofluorescence assay was used to detect the nuclear expression of γ-H2A.X, a marker protein of DNA damage. Western blot was employed to analyze the expression levels of oxidative damage?related proteins, including γ-H2A.X, RAD50, p53, and KEAP1. Results Following Garcinone C treatment, both HONE1 and HK1 cells exhibited significantly increased MDA content and lipid peroxidation levels (P < 0.05), along with a marked elevation in nuclear γ-H2A.X expression (P < 0.05). The protein expression levels of γ-H2A.X and p53 were significantly upregulated (P < 0.05), whereas KEAP1 and RAD50 protein expression levels were significantly downregulated (P < 0.05). Conclusion Garcinone C exerts anti?tumor effects by inducing oxidative damage in nasopharyngeal carcinoma cells and may serve as a promising lead compound for the treatment of nasopharyngeal carcinoma. |
| Key words: Garcinone C Nasopharyngeal carcinoma Oxidative damage |
