| 摘要: |
| ［摘要］ 目的 探讨糖尿病肾病发展过程中α-actinin-4的表达变化及贝那普利对其的干预作用。方法 建立STZ诱导的糖尿病大鼠模型，贝那普利对其干预，应用免疫组化及RT-PCR方法观察在糖尿病肾病发展过程中α-actinin-4的蛋白及mRNA表达变化。结果 8周时，糖尿病组染色强度较正常组明显增高（P<0.01），12周时变化更为明显（P<0.01）。α-actinin-4的mRNA的表达水平在各时间点与组化结果相同。贝那普利干预组8周时α-actinin-4表达较糖尿病组明显降低（P<0.05），而在12周时其表达仍较糖尿病组低，但无统计学意义。结论 α-actinin-4在糖尿病肾病发展过程中表达增高，贝那普利能下调其表达。 |
| 关键词: α-actinin-4 蛋白尿 |
| DOI： |
| 分类号:R 587.1 |
| 基金项目: |
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| The expression of α-actinin-4 in diabetic rats and the effect of benzapril on α-actinin-4 expression |
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YIN Yu-hong, LI Ying
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The First Centre Hospital in Baoding, Hebei 071000, China
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| Abstract: |
| ［Abstract］ Objective To investigate the change of α-actinin-4 expression and the effect of benzapril on α-actinin-4 expression.Methods STZ-induced diabetic rats were established, benzapril was given to it, immunohistochemical and RT-PCR were employed to study the change of α-actinin-4 protein and mRNA.Results At week 8, compared with normal group, the expression of α-actinin-4 protein in diabetic group increased dramatically（P<0.01）;At week 12, the expression still raised（P<0.01）.The expression of α-actinin-4 mRNA was similar to immunohistochemical staining. At week 8, the expression of α-actinin-4 protein in benzapril group decreased when compared with diabetic group（P<0.05）; At week 12, it can also decreased, but had no statistical significance.Conclusion The expression of α-actinin-4 increased in diabetic nephropathy and benzapril can normalize it. |
| Key words: α-actinin-4 Proteinuria |
