| 摘要: |
| ［摘要］ 目的 探讨适用于临床微生物实验室常规初筛获得性金属β内酰胺酶（MBLs）的实验方法，为指导临床合理选择抗生素提供依据。方法 以 EDTA为 MBLs 的抑制剂，以亚胺培南 IMP 和头孢他啶 CAZ 为 MBLs 的底物，采用双纸片增效法作 MBLs 初筛试验，将两种初筛试验的结果与聚合酶链反应 PCR 的结果进行比较分析。结果 应用 PCR法，在70株耐IMP和或 CAZ的鲍曼不动杆菌中检出 VIM型 MBLs 阳性株4 株，IMP型MBLs阳性株6株。IMP-EDTA纸片法的敏感性50%，特异性28.6%；CAZ-EDTA纸片的敏感性85.8%，特异性13.3%。结论 IPM-EDTA纸片法方法简便、结果可靠, 可作为获得性MBL的初筛方法在临床微生物室日常工作中开展。 |
| 关键词: 金属β内酰胺酶 亚胺培南 头孢他啶 表型检测法 PCR扩增 |
| DOI：10.3969/j.issn.1674-3806.2009.04.014 |
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| Study of methods of screening acquired metallo-β -lactamases |
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SHI Ying-hong, FENG Ping
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Mianyang Center for Disease Control and Prevention, Mianyang 621000, China
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| Abstract: |
| ［Abstract］ Objective To explore and establish a method for screening acquired metallo-β-lactamases (MBLs) in clinical microbiology laboratory.Methods Seventy Acinetobacter baumannii strains with an imipenem′s MIC of ≥8 μg/ml and a ceftazidime′s MIC of ≥64 μg/ml were selected for MBLs screening. Imipenem-or ceftazidime-EDTA double disks test were used to detect the metallo-β-lactamases production. BlaIMP and blaVIM were amplified by PCR.And the results were evaluated with those of PCR analyses.Results Of the 70 clinical strains, there were 10 strains tested positive for MBLs genes by PCR: 6 for blaIMP and 4 for blaVIM. The imipenem-EDTA disks＇s sensitivity was 50% and the specificity was 28.6%.The ceftazidime-EDTA disks＇s sensitivity was 85.8% and the specificity was 13.3%. Conclusion The inhibitor potentiated disk diffusion test using IMP-EDTA is a convenient and credible method for detecting acquired MBLs producer in clinical microbiology laboratory. |
| Key words: Metallo-β-lactamases Imipenem Ceftazidim MBLs screen PCR amplify |
