| 摘要: |
| ［摘要］ 目的 评价并比较酶联免疫吸附试验(ELISA)法检测ζ珠蛋白链与聚合酶链反应（PCR）方法在诊断广西东南亚缺失型α-地中海贫血中的临床诊断价值。方法 收集广西壮族自治区人民医院门诊部和产科住院病例210份血样标本（其中115例为正常对照；95例均有MCV<80 fl、MCH<27 pg，Hb在25～128 g/L之间），采用ELISA法和聚合酶链反应(PCR)方法进行检测, 最后对ELISA法筛查--SEA携带者总的敏感度、特异度、准确度、阴性预测值、阳性预测值与聚合酶链反应(PCR)方法进行分析并比较其诊断--SEA携带者的临床应用价值。结果 ELISA法诊断--SEA携带者总的敏感度、特异度、准确度、阴性预测值和阳性预测值分别是96.2%、98.3%、97.6%和98.3%、96.2%, 而聚合酶链反应(PCR)法检测的相应指标均为100.0%(P>0.01)，两者无显著性差异。ELISA法不但对--SEA携带者有很高的检出率, 而且还能检出个别-α/αα的病例, 但其不能筛查出β-地中海贫血，对脐带血的检出率低(仅为65.0%)。结论 ELISA法能非常有效地检出--SEA携带者，加之该法简便快速、技术要求不高且经济实用，故很适用于常规实验室和大样本的人群筛查。 |
| 关键词: α-地中海贫血 酶联免疫吸附试验 聚合酶链反应 |
| DOI：10.3969/j.issn.1674-3806.2009.12.18 |
| 分类号:R 566.6 |
| 基金项目: |
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| Application value of enzyme-linked immunosorbent assay(ELISA) for zeta-globin chains in screening of α-thalassemia Southeast Asian deletion in Guangxi |
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ZHANG Jin-li, HUANG Jun, LIU Xiao-chun, et al.
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Guangxi Center for Clinical Laboratory, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To compare the clinical diagnosis value between an enzyme-linked immunosorbent assay(ELISA) for embryonic zeta-globin chains and polymerase chain reaction(PCR) method as a routine screening test for(--SEA)α-thalassemia in clinical laboratory. Methods Two hundred and ten blood samples（115 cases normal; 95 cases MCV<80 fl、MCH<27 pg, Hb 25～128 g/L）from consecutive outpatient and obstetrical inpatients in The People′s Hospital of Guangxi Zhuang Autonomous Region with a request for hemoglobin (Hb) analysis were screened simultaneously for α-thalassemia determinants by ELISA and PCR respectively.Results There were very significant higher sensitivity, specificity, accuracy and negative predictive values as well as positive predictive values of ELISA method(96.2%, 98.3%, 97.6%, 98.3% and 96.2%, respectively) like PCR (100%, 100%, 100%, 100% and 100%, respectively) for screening for --SEA carrier(all P>0.01).The ELISA method was capable of detecting effectively only the cases with -α/αα. But it had a low positive rate in neonatal cord blood (only 65%).Conclusion The ELISA method is a highly sensitive and specific and reliable test for -α/αα and is more effective, The ELISA method is simple to perform, rapid, and applicable to large-scale or population-based screening programs and to a routine diagnostic laboratory. |
| Key words: α-Thalassemia Enzyme-linked immunosorbent assay Polymerase chain reaction |
