| 摘要: |
| ［摘要］ 目的 了解高糖对原代培养的人肾小球系膜细胞表达TGF-β1的影响，并进一步探讨糖尿病肾病的发病机制。方法 取自愿水囊引产的胎儿肾并解剖取肾皮质剪碎，应用肾皮质组织块法合优生选择法培养人肾小球系膜细胞。以ELISA方法检测TGF-β1的表达。结果 与正常组相比较，高糖组TGF-β1在24、48、72 h均显著升高(P<0.05或P<0.01)。结论 高糖能够升高系膜细胞TGF-β1分泌，这可能与肾小球系膜细胞细胞外基质积聚和糖尿病肾病发病密切相关。 |
| 关键词: 人系膜细胞 原代培养 转化生长因子β1 高糖 |
| DOI：10.3969/j.issn.1674-3806.2010.01.07 |
| 分类号:R 587.1 |
| 基金项目: |
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| Effects of high glucose on the expression of TGF-β1 in primary cultured human mesangial cells |
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YIN Yan-zhi, ZHANG Li-mei, MU Shou-han, et al.
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College of integrated Chinese and Western Medicine，Binzhou Medical University, Yantai 264003, China
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| Abstract: |
| ［Abstract］ Objective To observe the effect of high glucose on the expression of TGF-β1 in primary cultured human mesangial cells，and to elucidate their possible role in diabetic nephropathy.Methods Fetal kidneys from voluntary induction of labor with water bag were cut into pieces;Human mesangial cells were cultivated with the method of renal cortical tissue combined eugenic selection；ELISA was used to measure the expression of TGF-β1.Results Compared to the normal group, the expression of TGF-β1 in high glucose group were significantly high at 24 h, 48 h, 72 h(P<0.05 or P<0.01).Conclusion High glucose can increase the expression of TGF-β1 in mesangial cells, which may contribute to the inhibition of ECM degradation in glomerulus and the possibility of diabetic nephropathy. |
| Key words: Human mesangial cells Primary culture TGF-β1 High glucose |
