| 摘要: |
| ［摘要］ 目的 测定克泻灵胶囊总生物碱中槐果碱的含量。方法 以苯-丙酮-乙酸乙酯-甲醇(7∶3∶3∶0.5)为展开剂，在氨水饱和的碱性环境中，于硅胶GF254薄层板上分离槐果碱。荧光下定位，采用紫外分光光度法进行含量测定。结果 槐果碱在2.16～10.8 μg/ml(r=0.9999)范围内线性关系良好，提取回收效率为98.2%，RSD为0.57%(n=5)。结论 本法测定结果可靠、准确、重现性好，可用于该制剂的质量控制。 |
| 关键词: 克泻灵胶囊 苦豆草总生物碱 槐果碱 薄层层析 紫外光光度法 |
| DOI：10.3969/j.issn.1674-3806.2010.02.32 |
| 分类号:R 914.1 |
| 基金项目: |
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| Content determination of sophocarpine of kexieling capsule by TLC-UV |
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LIANG Xiu-qun
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Ruikang Hospital, Guangxi College of Traditional Chinese Medicine, Nanning 530011, China
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| Abstract: |
| ［Abstract］ Objective To determine content of sophocarpine of kexieling capsule.Methods Developing agent was benzene-acetonum-acetoacetate-acetoacetate (7∶3∶3∶0.5) and was saturated with aqueous ammonia, segregating sophocarpine using GF254 thin layer plate. Content of sophocarpine was determined by UV and located by fluorescence.Results There was a good linear relationship of sophocarpine in the range of 2.16～10.8 μg/ml (r=0.9999). The extraction recovery was 98.2%, RSD% was 0.57%(n=5).Conclusion The proposed method was simple, rapid, accurate and reliable, and can be used for content determination of sophocarpine. |
| Key words: Kexieling capsule Sophora alopecuridesl total alkaloids Sophocarpine TLC UV |
