| 摘要: |
| ［摘要］ 目的 探讨乙型肝炎病毒与DNA损伤的关系以及不同感染模式慢性乙型肝炎患者（大三阳和小三阳患者）DNA损伤情况。方法 应用单细胞凝胶电泳(SCGE)技术检测对照组和不同感染模式慢性乙型肝炎患者外周血淋巴细胞DNA的损伤情况，采用彗星分析软件对实验结果进行淋巴细胞损伤率、彗星尾长(Tail Extent)、彗星尾DNA百分含量(Tail %DNA)、彗星尾惯量(Tail Inertia)、Olive尾距(Olive Tail Moment)等指标进行分析。结果 经SCGE技术后得HBV感染与DNA损伤断裂关系有统计学意义(P<0.05)；大小三阳组间DNA损伤断裂有统计学差异(P<0.05)。 小三阳组经SCGE技术后所得彗星图像可见有少量彗星尾。大三阳组经SCGE技术后所得彗星图像可见有明显彗星尾。慢性乙型肝炎组外周血淋巴细胞DNA损伤与对照组比较彗星尾长、尾惯量在统计学上有显著性差异(P<0.05)；不同感染模式的乙型肝炎外周血淋巴细胞DNA损伤比较，尾长、尾DNA百分含量和尾惯量在统计学上有统计学差异(P<0.05)。结论 HBV感染可能造成外周血淋巴细胞DNA损伤，且不同感染模式的损伤情况不同。 |
| 关键词: 单细胞凝胶电泳 乙型肝炎病毒 DNA损伤 |
| DOI：10.3969/j.issn.1674-3806.2010.08.01 |
| 分类号:R 512.6 |
| 基金项目:国家自然科学基金项目（No.30860247);广西自然科学基金项目（桂科青0728066） |
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| Detection of DNA damage in peripheral blood lymphocytes of HBV-infected patients with single-cell gel electrophoresis |
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HUANG Jin-mei,ZENG Xiao-yun,QIU Xiao-qiang
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Pubilc Health College of Guangxi Medical University,Nanning 530021，China
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| Abstract: |
| ［Abstract］ Objective To explore the relationship between the hepatitis B virus and DNA damage, and explore DNA damage situation around the different mode of infection of HBV-infected patients.Methods Single-cell gel electrophoresis (SCGE) technique was adopted in measuring DNA damage in peripheral blood lymphocytes of controls, different models of chronic HBV-infected patients and the comet analysis software was used for analyzing the results. The degree of DNA damage was judged by the comet tail length (tail extent), the percentage of the comet tail DNA (Tail% DNA), the comet tail of inertia (tail inertia), olive tail moment.Results There were significantly differences between infection of HBV and DNA damage(P<0.05) ,and also significantly differences between the different mode of infection of HBV-infected patients(P<0.05) after the SCGE .There were a few comet tail images in lymphocytes of xiaosanyang group and obviously comet tail images in lymphocytes of dasanyang group . The tail extent , tail %DNA in lymphocytes of chronic hepatitis B patients were aggravated significantly than in lymphocytes of controls (P<0.05),and there were significantly differences between the tail extent , tail %DNA, tail inertia of the different mode of infection in HBV-infected patients（P<0.05）.Conclusion Hepatitis B virus is likely to be the main cause of resulting DNA damage in peripheral blood lymphocytes. And different mode of infection of HBV-infected patients have the different damage situation. |
| Key words: Single-cell gel electrophoresis Hepatitis B virus DNA damage |
