| 摘要: |
| ［摘要］ 目的 探讨体外培养大鼠外周血内皮前体细胞(EPCs)与自体骨髓基质细胞（BMSCs）共培养时对BMSCs成骨作用的影响。方法 采用密度梯度离心法分离、培养大鼠BMSCs和EPCs，培养细胞分为四组：A组(BMSCs组)、B组(EPCs组)、C组(BMSCs成骨诱导组)及D组(BMSCs和EPCs联合培养组)。通过观察细胞克隆形态、免疫细胞化学、细胞增殖、碱性磷酸酶活性,从酶学、组织学及生化等不同方面观察EPCs对BMSCs成骨活性及生长情况的影响。结果 免疫细胞化学染色证实C组培养的细胞具有晚期EPCs的特性。倒置相差显微镜、HE染色均证实共培养的BMSCs和EPCs生长良好,并能够形成与单纯成骨诱导培养的BMSCs相似的钙结节。MTT检测结果:各组细胞增殖差异无统计学意义(P>0.05)。碱性磷酸酶活性检测结果：C、D组显著高于A、B组(P<0.05)。结论 EPCs和BMSCs联合培养具有良好的细胞相容性，EPCs能够增强成骨细胞的ALP活性，提高成骨细胞的增殖能力。 |
| 关键词: 组织工程 骨髓基质细胞 内皮前体细胞 联合培养 |
| DOI：10.3969/j.issn.1674-3806.2011.05.11 |
| 分类号:R 78 |
| 基金项目: |
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| Influence of endothelial progenitor cells on osteogenic effects of autologous bone marrow stromal cells of rats in vitro co-culture condition |
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WEI Hui-ping,PANG Fang-he
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College of Stomatology， Guangxi Medical University, Nanning 530021,China
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| Abstract: |
| ［Abstract］ Objective To observe the influence of endothelial progenitor cells (EPCs) on the osteogenic effects of autologous bone marrow stromal cells (BMSCs) of rats in vitro co-culture of EPCs and BMSCs.Methods BMSCs and EPCs were obtained from rat bone marrow and peripheral blood by density gradient centrifugation.The cultured cells were divided into four groups：group A (BMSCs)，group B (EPCs osteogenic induction)，group C (BMSCs osteogenic induction) and group D(co-culture of induced BMSCs and EPCs).The EPCs were identified with immunocytochemistry staining. The cell proliferation and alkaline phosphatase (ALP) activity were observed to determine the effects of the osteogenic potential and cellular compatibility in each group.Results The cells were able to line up in the cord-like structure and showed CD31、vWF、positive in group B. There was good compatibility when EPCs and induced BMSCs were co-cultured. The activity of ALP in the group C and D was higher than the other two groups.Conclusion In direct co-culture system in vitro, the cellular compatibility is perfect. EPCs can improve ALP activity of induced osteoblasts. |
| Key words: Tissue engineering Bone marrow stromal cells(BMSCs) Endothelial progenitor cells Co-culture |
