| 摘要: |
| ［摘要］ 目的 探讨高压氧（HBO）联合抗震颗粒对帕金森病（PD）大鼠的神经保护治疗作用。方法 将采用6-羟基多巴胺(6-OHDA)成功制作的PD大鼠模型，随机分为模型组（A组）及HBO联合抗震颗粒组（B组），用分光光度计检测两组谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)的活性及丙二醛(MDA)含量的变化；用免疫组化法测定黑质酪氨酸羟化酶(TH)阳性神经元、胶原纤维酸性蛋白（GFAP）及Bcl-2、Bax蛋白的表达。结果 B组GSH-Px、SOD活性明显升高，MDA含量降低（P<0.05)。TH阳性神经元及Bcl-2蛋白表达升高（P<0.01或P<0.05)、GFAP平均光密度及Bax蛋白的表达降低（P<0.01或P<0.05)。结论 HBO联合抗震颗粒治疗PD大鼠，能抑制大鼠脑部氧化应激反应，提高大鼠的抗氧化及抑制细胞凋亡功能，是一种有效的神经保护和治疗措施。 |
| 关键词: 高压氧 抗震颗粒 帕金森病 神经保护 |
| DOI：10.3969/j.issn.1674-3806.2011.07.01 |
| 分类号:R 742.5 |
| 基金项目:广西自然科学基金资助项目（编号：桂科自0832209） |
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| Neuroprotective effect of hyperbaric oxygen combined with anti-parkinson granule in treating Parkinson′s disease rats |
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PAN Xiao-rong，HU Yu-ying，FAN Qiu-ping，et al.
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Department of Hyperbaric Oxygen,the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To explore the neuroprotective effect of hyperbaric oxygen（HBO） combined with anti-parkinson granule on Parkinson′s disease (PD) rats.Methods PD rat model was established by 6-OHDA.Model rats were randomly divided into 2 groups: model group（A） and treatment group(hyperbaric oxygen combined with anti-parkinson granule)（B）. The changes of the activities of SOD and GSH-Px， the content of MDA were determined by using immunohistochemistry and the changes of expression of TH、GFAP、Bcl-2 and Bax protein were determined by using immunohistochemistry in PD rats.Results The changes of ethology appeared obviously.The activities of SOD and GSH-Px significantly increased ，and the content of MDA decreased in HBO group compared to model group (P<0.05).The activities of TH， Bcl-2 significantly increased ，and the expression of GFAP、Bax protein content decreased in treatment groups compared with the model group (P<0.01 or P<0.05).Conclusion Hyperbaric oxygen combined with anti-parkinson granule can restrain the degree of oxidative stress, elevate its antioxidant abilities and significantly restrain apoptosis in substantia nigra. It is an effective neuroprotective and thempeutic measure for PD model rats. |
| Key words: Hyperbaric oxygen（HBO） Anti-parkinson granule Parkinson′s disease（PD） Neuroprotection |
