| 摘要: |
| ［摘要］ 目的 通过对人类白细胞抗原（HLA）-DR基因的检测，探讨哮喘儿童HLA-DRB基因型基因多态性与儿童哮喘易感性的关系。方法 将无血缘关系的84名哮喘儿童（哮喘组）和168名无哮喘和特应性疾病的健康个体（对照组）纳入研究。采用Pharmacia UniCAP系统检测哮喘儿童的血清总IgE水平，同时完成10种吸入性过敏原皮肤实验。应用基因芯片法检测HLA-DR的21个基因位点。结果 支气管哮喘组HLA-DRB1*070X基因和HLA-DRB1*11XX基因频率（分别为2.98%和13.69%）高于对照组（分别为0.3%和5.95%， χ2=6.915，P<0.05和χ2=9.478，P<0.01），优势比(OR)分别为10.57（95%CI 1.215～91.986）和2.79（95%CI 1.429～5.449）；HLA-DRB3(52)*010X基因频率在哮喘组为7.14%，低于对照组的13.99%（χ2=5.854，P<0.05），OR为0.429（95%CI 0.214～0.862）。结论 HLA-DRB1*070X基因和HLA-DRB1*11XX基因与哮喘儿童易感性相关;HLA-DRB3(52)*010X基因则为抗性基因。 |
| 关键词: 哮喘 人类白细胞抗原 免疫球蛋白E 基因多态性 儿童 |
| DOI：10.3969/j.issn.1674-3806.2011.09.06 |
| 分类号:R 725.6 |
| 基金项目:广西卫生厅自筹经费科研课题（编号：桂卫科Z2009167） |
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| Association between human leukocyte antigen DRB gene polymorphisms and asthma in children |
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XIE Qing-ling,ZHEN Hong,QIN Lin
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Department of Pediatrics,the People′s Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,China
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| Abstract: |
| ［Abstract］ Objective To investigate the association between human leukocyte antigen DRB (HLA-DRB) gene polymorphisms and asthma in children. Methods Eighty-four unrelated asthmatic individuals and one hundred and sixty-eight healthy controls without asthma and atopy were involved in the study. All asthmatics had their serum total IgE levels measured with UniCAP Pharmacia system, and skin-prick test with ten kinds of inhalant allergens were taken among the asthmatics. HLA oligonucleotide array was used to twenty-one gene frequencies of HLA-DR. Results The frequencies of HLA-DRB1*070X allele and HLA-DRB1*11XX allele among the asthmatics were 2.98% and 13.69%, significantly higher than those in healthy controls (0.3% and 5.95%, χ2=6.915, P<0.05 and χ2=9.478, P<0.01)）, Odds ratios (Ors) were 10.57（95%CI: 1.215～91.986） and 2.79（95%CI: 1.429～5.449） respectively. HLA-DRB3(52)*010Xallele were significantly decreased in asthmatics compared to healthy controls (13.99%, χ2=5.854, P<0.05), Ors were 0.429（95% CI:0.214～0.862）. Conclusion HLA-DRB1*070X allele and HLA-DRB1*11XX allele were implicated in susceptibility to asthma, HLA-DRB3(52)*010X allele might confer protection against asthma. |
| Key words: Asthma Human leukocyte antigen (HLA) IgE Gene polymorphism Children |
