| 摘要: |
| ［摘要］ 目的 了解河南省和云南省HIV-1病毒env基因在脑脊液及血液中的亚型变化。方法 收集6例河南省和5例云南省HIV-1阳性病人的血浆及脑脊液标本并提取RNA，逆转录为模版DNA后，采用巢式PCR法扩增env基因，对扩增产物进行核苷酸序列测定，构建系统进化树并进行离散度分析。结果 云南省各基因亚型与CRF07-BC、CRF08-BC和C亚型相近，河南省各基因亚型与B和B’亚型相近；河南人群脑脊液的测序结果显示患者1的部分克隆株与患者5的基因亚型一致，云南人群中12号患者和127号患者的血液及脑脊液的病毒亚型来自不同的分支，而其他患者的血液与脑脊液的病毒亚型一致。河南省HIV-1各亚型分离株脑脊液和血液间基因离散度差异有统计学意义(P<0.05)。结论 云南省HIV-1基因亚型为C亚型，而河南省为B和B’亚型。脑脊液和血液中的病毒亚型存在变异，中枢神经系统中HIIV病毒的离散度低于血液中HIIV病毒的离散度。 |
| 关键词: 人类免疫缺陷病毒 基因亚型 序列分析 进化树 |
| DOI：10.3969/j.issn.1674-3806.2013.04.01 |
| 分类号:R 512.91 |
| 基金项目:国家自然科学青年基金资助项目（编号：81200848） |
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| Genotype analysis of HIV-1 env gene in cerebrospinal fluid and plasma of infected Chinese patients in Henan and Yunnan Provinces |
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LI Li，ZHAO Huan-ying, WU Yan-ning，et al.
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Laboratory of STD and AIDS, Beijing You’an Hospital，Capital Medical University, Beijing 100069，China
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| Abstract: |
| ［Abstract］ Objective To monitor the epidemic subtypes and evolution of HIV-1 in cerebrospinal fluid fluid and plasma of infected chinese patients in Henan and Yunnan Povinces by analyzing env gene.Methods The C2-V5 regions of the env gene was amplified by nested PCR following RNA reverse transcription extracted from both cerebrospinal fluid (CSF) and blood samples of HIV-1 positive patients. The amplicons were then sequenced，and the genetic subtype was identified by comparative analysis. The phylogenetic tree was constructed.Results Sequence analysis revealed that the sequences from Yunan Province clustered closely with subtypes CRF07-BC, CRF08-BC, and C, and Henan with subtypes B and B’. Some CNS clonal sequences from Patient 1 of Henan Province fell on different branches and clustered closely with the sequences of Patient 5; The sequences from the CSF and blood in patients 12 and 127 from Yunnan Province were all monophyletic and fell on different branches, but in the other three patients sequences from the CSF and blood were intermingled with each other.The levels of diversity revealed significant difference between the CSF and plasma samples from Henan patients（P<0.05）.Conclusion HIV-1 gene C subtype prevailed in Yunnan Province, while B and B’ subtypes prevailed in Henan Province. The sequences from blood showed a significantly higher divergence from those from the CSF. |
| Key words: HIV Gene subtype Sequence analysis Phylogenetic tree |
