| 摘要: |
| ［摘要］ 目的 探讨EB病毒Rta蛋白IgG抗体在鼻咽癌诊断中的临床价值。方法 采用间接ELISA法检测612例临床样本中EB病毒Rta-IgG含量，同时对试剂盒的分析性能进行评估。结果 267例鼻咽癌样本的EB病毒Rta-IgG抗体浓度为(77.64±3.205)U/ml，检测灵敏度为94.7%(214/226),特异度为86.3%(333/386)，正确率为89.4%(547/612)；255例健康体检血样抗体浓度为(8.31±0.745)U/ml；20例肝癌样本抗体浓度为(7.14±1.111)U/ml；30例肝炎样本抗体浓度为(7.40±0.581)U/ml；20例自身免疫病样本抗体浓度为(13.97±6.943)U/ml；20例鼻咽息肉样本抗体浓度为(12.58±6.197)U/ml。各组EB病毒Rta-IgG含量比较差异有统计学意义（P<0.05)。结论 EB病毒Rta-IgG定量检测试剂盒用于鼻咽癌诊断具有较高的临床价值，为进一步研究鼻咽癌患者治疗效果与患者血清中EB病毒Rta-IgG的含量的关系奠定了基础。 |
| 关键词: EB病毒Rta-IgG 定量检测 鼻咽癌 诊断 |
| DOI：10.3969/j.issn.1674-3806.2013.10.05 |
| 分类号:R 730.4 |
| 基金项目:广西卫生厅科研课题（编号：桂卫Z2010248） |
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| Performance evaluation of EB virus Rta-IgG quantitative detection kit and its application in diagnosing nasopharyngeal carcinoma |
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QIN Gui-fang，LI You -qiong，LU Qiu-wei，et al.
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Department of Clinical Laboratory, the People′s Hoespital of Guangxi Zhuang Autonomous Regiom,Nanning 530021,China
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| Abstract: |
| ［Abstract］ Objective To investigate the clinical value of Epstein-Barr virus (EBV) Rta/IgG in the diagnosis of nasopharyngeal carcinoma(NPC).Methods Sera from 612 clinical samples were measured for the Rta-IgG by an indirect enzyme-linked immunosorbent assay. In the present study, we also evaluated the performance of the Rta-IgG ELISA kits.Results The mean value of Rta-IgG concentration in 267 NPC patients was (77.64±3.205)U/ml with a sensitivity of 94.7%(214/226) and specificity of 86.3%(333/386) and accuracy of 89.4%(547/612). The mean value of Rta-IgG concentration in 255 healthy volunteers，20 hepatocellular carcinoma patients，30 hepatitis patients，20 patients with autoimmune disease and 20 patients with nasopharyngeal polyp were (8.31±0.745)U/ml, (7.14±1.111)U/ml, (7.40±0.581)U/ml, (13.97±6.943)U/ml and (12.58±6.197)U/ml, respectively. There were significant difference between NPC group and other groups.Conclusion Our results suggest that the EBV Rta/IgG ELISA kit is suitable for the clinical diagnosis of NPC. It also provides a basis for further study on the relationship between the therapeutic effect and serum EBV Rta-IgG content in NPC patients. |
| Key words: EBV Rta-IgG Quantitative detection Nasopharyngeal carcinoma（NPC） Diagnosis |
