| 摘要: |
| ［摘要］ 目的 观察腺病毒（Ad-11β-HSD1）过表达系统及特异性siRNA（siRNA-11β-HSD1）干扰人肝癌HepG2细胞对11β-羟基类固醇脱氢酶I（11β-HSD1）表达的影响。方法 应用Ad-11β-HSD1以及siRNA-11β-HSD1转染人肝癌HepG2细胞，用实时荧光定量PCR及Western Blot检测11β-HSD1表达。结果 转染72 h后，对照组及腺病毒过表达组细胞中均可见明显GFP荧光，且转染48 h后蛋白水平明显升高；siRNA干扰48 h后，mRNA及蛋白水平均明显降低。结论 腺病毒转染可有效过表达11β-HSD1，而siRNA干扰可有效抑制11β-HSD1表达。 |
| 关键词: HepG2 腺病毒 siRNA干扰 11β-羟基类固醇脱氢酶I |
| DOI：10.3969/j.issn.1674-3806.2014.06.03 |
| 分类号:R 44 |
| 基金项目:国家自然科学基金资助项目（编号：81060199） |
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| The effects of overexpression and siRNA interference of the human hepatocellular carcinoma cell line HepG2 on the expression of 11β-hydroxysteroid dehydrogenase1 |
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SUI Jing-zhe, LI Guo-ping, LI Shan
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Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To design a adenovirus vector based system and specific siRNA for exploring the effects of overexpression and siRNA interference of the human hepatocellular carcinoma cell line HepG2 on the expression of 11β-hydroxysteroid dehydrogenase1.Methods A adenovirus overexpression vector and specific siRNA targeting the 11β-HSD1 gene was designed and transfected into HepG2 cells. Expression of 11β-HSD1 mRNA and protein was analyzed by real time PCR and Western blotting.Results A adenovirus transfection resulted in upgrade of 11β-HSD1 protein, and siRNA transfection led to inhibition of 11β-HSD1 mRNA and protein.Conclusion Transfection of the hepatocellular carcinoma cell line HepG2 using a adenovirus vector and siRNA interference can significantly influence expression of 11β-HSD1 mRNA and protein. |
| Key words: HepG2 Adenovirus siRNA interference 11β-HSD1 |
