| 摘要: |
| ［摘要］ 目的 研究P53基因表达与食管癌同步放化疗敏感性的关系，为预测食管癌治疗效果提供参考依据。方法 采用RT-PCR方法检测50例食管癌患者外周血白细胞中P53基因mRNA的表达情况。入组病例均予以总量为60～70 Gy的三维适形调强放疗及2周期同步DF（顺铂+五氟尿嘧啶）方案化疗，分析P53基因表达与同步放化疗疗效之间的关系。结果 P53阳性组18例中，完全缓解（CR）4例，部分缓解（PR）8例，稳定（SD）4例，进展（PD）2例，治疗有效率为66.7%；P53阴性组32例中，CR 11例，PR 20例，SD 1例，PD 0例，治疗有效率为96.9%。两组疗效比较差异有统计学意义(P<0.05)。结论 食管癌病例中P53基因的过度表达可能影响肿瘤细胞对放射治疗及化学疗法的敏感性,检测食管癌P53基因的表达可能有助于判断食管癌对于放化疗的反应,并借此判断患者的预后,对于指导临床肿瘤治疗具有一定的参考意义。 |
| 关键词: 食管癌 P53 基因表达 聚合酶链反应 |
| DOI：10.3969/j.issn.1674-3806.2015.01.10 |
| 分类号:R 735 |
| 基金项目: |
|
| Study on the relationship between expression of gene P53 and sensitivity of esophageal carcinoma to concurrent radiotherapy and chemotherapy |
|
JIANG Zhou, HUANG Jiang-qiong, XU Yang, et al.
|
|
Department of Oncology, the Worker Hospital of Liuzhou City, Guangxi 545005, China
|
| Abstract: |
| ［Abstract］ Objective To explore the relationship between gene P53 expression and sensitivity of esophageal carcinoma to the concurrent radiotherapy and chemotherapy，and provide a prediction for the treatment of esophageal carcinoma.Methods The expression of P53 mRNA in peripheral blood leukocytes of 50 patients with esophageal carcinoma was detected by RT-PCR(reverse transcription-PCR) method. Patients were treated by 60～70 Gy radiotherapy and two cycles of conventional synchronous DF(fluorouracil and cisplatin) chemotherapy. The relationship between P53 gene expression and therapeutic effect of radiotherapy with concurrent chemotherapy was analyzed.Results The group of P53-positive patients(n=18) showed complete recovery(CR) in 4 cases, partly recovery(PR) in 8 cases, stability(SD) in 4 cases, progression of disease(PD) in 2 cases.The group of P53- negative patients(n=32) showed CR in 11 cases,PR in 20 cases,SD in 1 case, none of PD case.The efficacy of P53- negative patients was significantly better than that of P53-positive patients(P<0.05).Conclusion The over-expression of P53 gene may influence the sensitivity of tumor cells to radiotherapy and chemotherapy.Therefore, detecting the expression of P53 gene may help to determine the response of esophageal carcinoma to the radiotherapy and chemotherapy， and to determine the prognosis of patients. It has a value for clinical cancer therapy. |
| Key words: Esophageal carcinoma P53 Gene expression Polymerase chain reaction(PCR) |
