| 摘要: |
| ［摘要］ 目的 建立慢性间歇低氧并高血压SD大鼠模型，为研究阻塞性睡眠呼吸暂停低通气综合征（OSAHS）并高血压的发病机制提供简单可复制的动物模型。方法 氧舱系统分为控制系统、气源系统、暴露舱三个部分。选8～10周龄SD雄性大鼠24只（200～250 g）随机分成间歇低氧干预组和对照组。间歇低氧干预组12只暴露于间歇低氧舱内，白天给予间歇低氧干预（6%～8%的O2 40 s/21%的O2 80 s，如此往复循环8 h）；对照组12只暴露于常氧舱。在安静状态下检测大鼠尾动脉收缩压，每只连续测量3次,取平均值。结果 实验第35天，间歇低氧干预组尾动脉收缩压［（136.21±32.46）mmHg］高于对照组［（121.37±35.37）mmHg］（P<0.05）。结论 控制精确气源通断时间，可有效地模拟间歇低氧过程，重复性佳，是研究间歇低氧所致高血压机制的理想实验模型。 |
| 关键词: 间歇低氧 高血压 SD大鼠 |
| DOI：10.3969/j.issn.1674-3806.2015.11.01 |
| 分类号:R 544.1 |
| 基金项目:国家自然科学基金资助项目（编号：81160014）；广西自然科学基金资助项目（编号：2014GXNSFAA118261） |
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| A hypertension rat model induced by chronic intermittent hypoxia |
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JIANG Li-jun, LIU Jian-hong, SONG Wei, et al.
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Sleep Disordered and Breathing Center of Guangxi, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To establish a hypertension rat model induced by chronic intermittent hypoxia, and to provide a simple and duplicate animal model for the study of the pathogenesis of hypertension in OSAHS.Methods The intermittent hypoxia system was used to conduct the experiment, including the control subsystem, the gas supply subsystem and the exposed cabin. 24 male Sprague-Dawley rats, aged 8 to 10 weeks, and weighted from 200 to 250 g were randomly divided into two groups: the intermittent hypoxia intervention group and the control group. The intermittent hypoxia intervention group(n=12) was exposed to 6% to 8% oxygen for 40 seconds and then to 21 % oxygen for 80 seconds in the exposed cabin for 8 hours per day with many intermittent hypoxia cycles. The control group(n=12) was exposed to certain concentration oxygen chamber. The tail artery systolic pressure of the rats was taken at rest states for three consecutive times and the average was calculated.Results The intermittent hypoxia intervention group had a significantly higher systolic blood pressure than the control group at the 35th day of the experiment［（136.21±32.46）mmHg vs (121.37±35.37）mmHg, P<0.05］.Conclusion Precisely aerating the oxygen on and off in an certain range of concentration can effectively make a simulated intermittent hypoxia process, which is an ideal animal model for studying the mechanisms of intermittent hypoxia-induced hypertension. |
| Key words: Intermittent hypoxia Hypertension Sprague-Dawley rats |
