| 摘要: |
| ［摘要］ 圆形精子细胞注射（ROSI）技术是通过显微注射技术将单倍体圆形精子细胞注射入成熟卵母细胞中，使卵母细胞受精进而分裂形成胚胎的技术，是目前部分非梗阻性无精子症患者获得自己遗传学后代的最后希望。圆形精子细胞为单倍体雄性生殖细胞，是精原细胞经过减数分裂后变形成为精子前的最后一个阶段，主要通过睾丸穿刺取精术（TESA）或显微外科睾丸取精术（Micro-TESE）获得。虽然已经有ROSI试管婴儿出生，但总体来说ROSI技术效率（妊娠率、活产率）较低，因此到目前为止国内外还没有广泛推行该技术，其中不能准确鉴别活体单倍体圆形精子细胞是导致ROSI技术效率低下的主要原因之一。该文对目前几种鉴别人睾丸单倍体圆形精子细胞的方法进行综述。 |
| 关键词: 无精子症 圆形精子细胞 男性不育 圆形精子细胞注射技术 |
| DOI：10.3969/j.issn.1674-3806.2020.11.26 |
| 分类号:R 698.2 |
| 基金项目:广西卫健委科研课题（编号：Z20180742） |
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| Research progress in identification techniques of human testis haploid round spermatid |
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MAO Xian-bao, XUE Lin-tao
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Center for Reproductive Medicine and Genetics, the People′ s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Round spermatid injection(ROSI), a technique by which haploid round spermatids are injected into mature oocytes through microinjection to fertilize the oocytes and then divide them into embryos, is the last hope that non-obstructive azoospermia patients may obtain their own genetic offsprings. Round spermatids, haploid male germ cells, the last stage before spermatogonia undergo meiosis to form sperm, and are collected mainly through testicular sperm aspiration(TESA) or microscopic testicular sperm extraction(Micro-TESE). Although ROSI test-tube babies have been born, the ROSI technology efficiency(pregnancy rate, live birth rate) is generally low, and so far this technology has not been widely implemented at home and abroad. The inaccurate identification of living haploid round spermatids is one of the main reasons for the inefficiency of ROSI. This paper introduces several methods to identify human testis haploid round spermatid. |
| Key words: Azoospermia Round spermatid Male infertility Round spermatid injection(ROSI) technology |
