| 摘要: |
| ［摘要］ 目的 探讨光照强度对光学离焦性近视豚鼠的眼生物学参数、视网膜结构及巩膜组织光蛋白聚糖（lumican）和基质金属蛋白酶-2（MMP-2）表达水平的影响。方法 选择健康3周龄豚鼠80只，雌雄不限。采用凹透镜诱导方法构建右眼光学离焦性低中度近视豚鼠模型，从造模成功的74只豚鼠中随机选取60只分为A组、B组、C组和D组，每组15只。A组每日置于室外充足阳光下3 h，平均光照度60 000 lux；B组每日置于室外阴凉处3 h，平均光照度10 000 lux；C组每日置于室内北窗处3 h，平均光照度2 000 lux；D组每日室内正常饲养，平均光照度300 lux。其余时间统一室内饲养，平均光照度300 lux，正常摄食饮水，持续光照处理6个月。比较各组豚鼠眼生物学参数（眼轴长度、屈光度），观察视网膜病理和超微结构，并检测巩膜组织lumican和MMP-2表达水平。结果 与光照前比较，光照1个月、3个月及6个月时各组屈光度有不同程度增加；A组光照3个月、6个月，B组、C组和D组光照1个月、3个月及6个月时眼轴长度有不同程度增长，差异有统计学意义（P<0.05）。光照1个月时，A组和B组屈光度、眼轴长度低于D组；光照3个月、6个月时，A组屈光度、眼轴长度低于B组、C组和D组，B组屈光度、眼轴长度低于D组，差异有统计学意义（P<0.05）。A组和B组凋亡细胞数量少于C组、D组，且A组凋亡细胞数量少于B组，差异有统计学意义（P<0.05）。A组和B组视网膜结构损伤程度较C组、D组轻，且A组损伤程度较B组轻。光照6个月后，与C组、D组比较，A组和B组的lumican表达水平较高，MMP-2表达水平较低，且A组lumican表达水平高于B组，MMP-2表达水平低于B组，差异有统计学意义（P<0.05）。结论 室外高强度光照能促使豚鼠巩膜组织中lumican表达上调，MMP-2表达下调，稳固巩膜结构，具有减缓眼轴增长和近视进展的作用。 |
| 关键词: 光照强度 近视进展 光蛋白聚糖 基质金属蛋白酶 视网膜结构 |
| DOI：10.3969/j.issn.1674-3806.2024.03.10 |
| 分类号:R 778.11 |
| 基金项目:秦皇岛市科学技术研究与发展计划项目（编号：202301A190） |
|
| Effects of illumination intensity on eye biological parameters, retinal structure, and expression levels of lumican and MMP-2 in scleral tissues of myopic guinea pigs |
|
FU Junda, ZHU Haiping, YANG Wanchen, JIA Na, SUN Yijun
|
|
Department of Ophthalmology, First Hospital of Qinhuangdao, Hebei 066000, China
|
| Abstract: |
| ［Abstract］ Objective To explore the effects of illumination intensity on eye biological parameters, retinal structure, and expression levels of lumican and matrix metalloproteinase-2(MMP-2) in scleral tissues of optical defocus myopia guinea pigs. Methods A total of 80 healthy 3-week-old guinea pigs were selected, regardless of gender. The models of guinea pigs with right-eye optical defocused low to moderate myopia were constructed by concave lens induction method. Among them, 60 guinea pigs were randomly selected from 74 successfully modeled guinea pigs and divided into group A, group B, group C and group D, with 15 guinea pigs in each group. The group A was exposed to outdoor sufficient sunlight for 3 hours every day and the average illumination was 60 000 lux. The group B was placed in a cool outdoor place for 3 hours every day and the average illumination was 10 000 lux. The group C were placed at the indoor north window for 3 hours every day and the average illumination was 2 000 lux. The group D was raised indoors normally every day with an average illumination of 300 lux. In the rest of the time, all the guinea pigs were given the same indoor feeding with an average illumination of 300 lux, normal food and water intake, and continuous illumination treatment for 6 months. The ocular biological parameters(axial length and diopter) of the guinea pigs were compared among different groups, and the retinal pathology and ultrastructure of the guinea pigs were observed, and the expression levels of lumican and MMP-2 in the scleral tissues were detected. Results Compared with those before light exposure, the diopter increased in different degrees in each group after 1 month, 3 months and 6 months of light exposure, and the group A showed varying degrees of increase in axial length at 3 and 6 months of light exposure, while the group B, the group C and the group D showed varying degrees of increase in axial length at 1, 3, and 6 months of light exposure, and the differences were significant(P<0.05). After 1 month of light exposure, the ocular diopter and axial length in the group A and the group B were lower than those in the group D, and after 3 and 6 months of light exposure, the ocular diopter and axial length in the group A were lower than those in the group B, the group C and the group D, and the ocular diopter and axial length in the group B were lower than those in the group D, and the differences were significant(P<0.05). The number of apoptotic cells in the group A and the group B was less than that in the group C and the group D, and the number of apoptotic cells in the group A was less than that in the group B, and the differences were significant(P<0.05). The damage degree of retinal structure in the group A and the group B was milder than that in the group C and the group D, and the damage degree in the group A was milder than that in the group B. After 6 months of light exposure, compared with those in the group C and the group D, the expression levels of lumican were higher and the expression levels of MMP-2 were lower in the group A and the group B, and the expression levels of lumican in the group A were higher than those in the group B, and the expression levels of MMP-2 in the group A were lower than those in the group B, and the differences were significant(P<0.05). Conclusion Outdoor high-intensity light exposure can up-regulate lumican expression and down-regulate MMP-2 expression in the scleral tissues of guinea pigs, and can stabilize scleral structure, which can slow down the growth of ocular axial length and myopia progression. |
| Key words: Illumination intensity Myopia progression Lumican Matrix metalloproteinase Retinal structure |
