| 摘要: |
| ［摘要］ 目的 探究转化生长因子-β（TGF-β）信号通路对牙源性黏液瘤（OM）来源的间充质干细胞（OMMSC）成骨分化的影响。方法 选择2018年至2020年南京大学医学院附属口腔医院收治的OM患者2例，经手术取部分病灶标本进行OMMSC分离培养。另外选择于该院接受牙种植治疗的年轻患者5例，抽取颌骨骨髓进行颌骨骨髓来源的间充质干细胞（JBMSC）分离培养。通过细胞增殖实验、流式细胞术检测、茜素红S染色进行细胞鉴定。成骨培养7 d后通过实时荧光定量聚合酶链反应（RT-PCR）检测TGF-β信号通路以及成骨相关基因的表达情况。观察小分子药物SB431542及TGF-β1干预对OMMSC、JBMSC成骨分化能力的影响。结果 经分离获得的OMMSC、JBMSC具有梭形形态，表达间充质干细胞表面标志物，并具有成骨分化能力，符合间充质干细胞的特征。与JBMSC相比，OMMSC具有更强的增殖能力。RT-PCR检测结果显示，TGF-β/Smad信号相关因子在OMMSC中呈高表达（P<0.05），成骨相关因子骨桥蛋白（OPN）表达降低（P<0.05）。茜素红S染色结果显示SB431542可显著促进OMMSC成骨分化，而TGF-β1对OMMSC成骨分化有抑制作用。结论 该研究成功对OMMSC进行了分离，发现TGF-β信号相关因子在OMMSC中呈高表达，抑制TGF-β信号转导通路可增强OMMSC的成骨能力，为改善OM患者骨缺陷提供参考。 |
| 关键词: 牙源性黏液瘤来源的间充质干细胞 颌骨骨髓来源的间充质干细胞 成骨分化 转化生长因子-β信号通路 |
| DOI：10.3969/j.issn.1674-3806.2024.04.10 |
| 分类号:R 739.8 |
| 基金项目:江苏省重点专科项目（编号：CZ0009） |
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| Analysis on the effect of TGF-β signaling pathway on osteogenic differentiation of odontogenic myxoma mesenchymal stem cells |
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CHEN Xinglin1, WANG Zhongshan2, SUN Guowen3, CHEN Sheng4, TONG Xin3, QIN Haiyan5
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1.Department of Dental Implantology, Stomatological Hospital Affiliated to Medical School of Nanjing University(Nanjing Stomatological Hospital, Nanjing University Institute of Stomatology), Jiangsu 210000, China; 2.Department of Prosthodontics, Stomatological Hospital of Fourth Military Medical University, Xi′an 710000, China; 3.Department of Oral and Maxillofacial Surgery, Stomatological Hospital Affiliated to Medical School of Nanjing University(Nanjing Stomatological Hospital, Nanjing University Institute of Stomatology), Jiangsu 210000, China; 4.Department of Oral Pathology, Stomatological Hospital Affiliated to Medical School of Nanjing University(Nanjing Stomatological Hospital, Nanjing University Institute of Stomatology), Jiangsu 210000, China; 5.Department of Stomatology, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Jiangsu 210000, China
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| Abstract: |
| ［Abstract］ Objective To explore the effect of transforming growth factor-β(TGF-β) signaling pathway on osteogenic differentiation of odontogenic myxoma(OM) mesenchymal stem cells(OMMSC). Methods Two patients with OM who were admitted to Stomatological Hospital Affiliated to Medical School of Nanjing University from 2018 to 2020 were selected. The patients′ partial lesion specimens were obtained through surgery for OMMSC isolation and culture. In addition, 5 young patients who received dental implant therapy in the hospital were selected to extract bone marrow from the jaw bone for jaw bone mesenchymal stem cells(JBMSC) isolation and culture. The cells were identified by cell proliferation assay, flow cytometry and alizarin red S staining. After 7 days of osteogenic culture, real-time fluorescence quantitative polymerase chain reaction(RT-PCR) was used to detect the TGF-β signaling pathway and the expression of osteogenesis-related genes. The effects of small molecule drug SB431542 and TGF-β1 interventions on osteogenic differentiation ability of OMMSC and JBMSC were observed. Results OMMSC and JBMSC obtained by isolation had spindle-shaped morphology, and expressed the surface markers of mesenchymal stem cells, and had osteogenic differentiation ability, which were consistent with the characteristics of mesenchymal stem cells. Compared with JBMSC, OMMSC had stronger proliferation ability. The results of RT-PCR showed that TGF-β/Smad signal-related factors were highly expressed in OMMSC(P<0.05), and the expression of osteogenesis-related factor osteopontin(OPN) was decreased(P<0.05). The results of alizarin red S staining showed that SB431542 could significantly promote the osteogenic differentiation of OMMSC, while TGF-β1 inhibited the osteogenic differentiation of OMMSC. Conclusion In this study, OMMSC are successfully isolated, and TGF-β signal-related factors are highly expressed in OMMSC, and inhibiting TGF-β signal transduction pathway can enhance the osteogenic ability of OMMSC, which provides reference for improving bone defects in patients with OM. |
| Key words: Odontogenic myxoma mesenchymal stem cells(OMMSC) Jaw bone mesenchymal stem cells(JBMSC) Osteogenic differentiation Transforming growth factor-β(TGF-β) signaling pathway |
