| 摘要: |
| 目的 探讨微量采血管对原子吸收光谱法检测Cu、Zn、Ca、Mg、Fe的影响。方法 分别用微量采血管和移液器吸样检测40份全血样本,分为微量采血组和移液器吸样组；对40份用微量采血管浸泡于稀释液后的样本进行检测,设为浸泡组,在相同条件下检测40份不加任何样品的试剂空白为对照组。比较浸泡组与对照组、微量采血组和移液器吸样组检测结果的差异。结果 微量采血管浸泡组中Zn、Ca、Fe的含量明显高于试剂空白对照组(P<0.01)。与移液器吸样组相比,微量采血组检测结果中Zn、Ca浓度明显升高(P<0.01)。结论 微量采血管对微量元素检测中的Zn、Ca结果影响较大,在进行微量元素检测时有必要做好空白对照以尽可能减少相关影响。 |
| 关键词: 原子吸收光谱法 微量元素 微量采血管 |
| DOI： |
| 分类号: |
| 基金项目:广西医疗卫生适宜技术研究与开发项目(编号：S2014 22-03) |
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| Influence of micropipet on detection of Cu、Zn、Ca、Mg、Fe |
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Yang wen-hui, Yuan yu-lin
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the People’s Hospital of Guangxi Zhuang Autonomous Region
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| Abstract: |
| Objective evaluate the influence of micropipet on atomic absorption spectrometry(AAS)to detect the levels of copper(Cu),zinc(Zn),calcium(Ca),magnesium(Mg),iron(Fe),based on the method of AAS. Methods 40 blood samples were detected by use micropipet and pipette suction samples. Results the levels of Zn,Ca,Fe in the micropipet soak were significantly higher than that of reagent blank(P<0.01).Compared with use pipette suction samples,use the micropipet detected the levels of Zn and Ca were significantly increased(P<0.01). Conclusion the micropipet have significant influence on the resuls,if it is necessary to use the micropipet we must have blank control to minimize the impact on the trace elements detect. |
| Key words: atomic absorption spectrometry trace elements micropipet |
