| 引用本文: | 丘柳柳,谢深霞,杨晓梅,卢小玲.牙髓干细胞条件培养基联合LDH纳米材料抗HaCaT细胞光老化的作用研究[J].中国临床新医学,0,():-. |
| QIU Liuliu.牙髓干细胞条件培养基联合LDH纳米材料抗HaCaT细胞光老化的作用研究[J].中国临床新医学,0,():-. |
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| 摘要: |
| 目的 探究牙髓干细胞条件培养基联合LDH纳米材料对光老化人永生化角质形成细胞（HaCaT细胞）的影响，并初步探讨其机制。方法 制备牙髓干细胞条件培养基。合成与表征LDH纳米材料。除对照组和模型组外，分别用牙髓干细胞条件培养基（DPSCs-CM）、LDH纳米材料（LDH）以及牙髓干细胞条件培养基联合LDH纳米材料（DPSCs-CM+LDH）预处理HaCaT细胞24 h。预处理后，除对照组外，其余组HaCaT细胞用中波紫外线（UVB）照射。照射后立即采用相应试剂盒检测各组HaCaT细胞中的丙二醛（MDA）含量、超氧化物歧化酶（SOD）活力以及活性氧（ROS）水平，采用细胞划痕实验检测各组HaCaT细胞的迁移能力。照射后72 h，采用Western Blot法检测各组HaCaT细胞中p53和p16蛋白的表达水平。结果 成功合成了具有良好稳定性和分散性的LDH纳米材料。体外功能实验结果显示，接受UVB照射的HaCaT细胞经牙髓干细胞条件培养基联合LDH纳米材料（DPSCs-CM+LDH）预处理后，细胞中的ROS水平和MDA含量降低、SOD活力升高，细胞迁移能力提高，细胞内p53和p16蛋白的表达水平降低（P＜0.01）。结论 牙髓干细胞条件培养基联合LDH纳米材料预处理可以提高单独应用牙髓干细胞条件培养基抗HaCaT细胞光老化的疗效，为抗皮肤光老化的体外研究提供了新的思路。 |
| 关键词: 牙髓干细胞条件培养基 层状双金属氢氧化物 HaCaT细胞 光老化 |
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| 基金项目:国家自然科学基金资助项目(编号:82260614) |
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| The anti-photoaging effect of dental pulp stem cells-conditioned medium combined with LDH nanomaterials on HaCaT cells |
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QIU Liuliu
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1.College &2.Hospital of Stomatology，Guangxi Medical University
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| Abstract: |
| Objective To investigate the effect of dental pulp stem cells-conditioned medium(DPSCs-CM) combined with LDH nanomaterials on photoaged human immortalized keratinocytes(HaCaT cells) and explore the underlying mechanism. Methods The DPSCs-CM was prepared. The LDH nanomaterials were synthesized and characterized. Except for the control group and model group, HaCaT cells were pretreated with DPSCs-CM, LDH nanomaterials(LDH), or DPSCs-CM combined with LDH nanomaterials(DPSCs-CM+LDH) for 24 hours. After pretreatment, except for the control group, the rest of the HaCaT cells were exposed to medium-wave ultraviolet(UVB) radiation. Immediately post irradiation, the malonaldehyde(MDA) content, superoxide dismutase(SOD) activity and reactive oxygen species(ROS) levels in the HaCaT cells of each group were measured using the corresponding detection kit, and the migration ability of HaCaT cells in each group was detected by cell scratch assay. After 72 hours irradiation, the expression levels of p53 and p16 proteins in the HaCaT cells of each group were detected by Western Blot. Results The LDH nanomaterials with good stability and dispersibility were successfully synthesized. In vitro functional experiments showed that HaCaT cells pretreated with DPSCs-CM combined with LDH nanomaterials(DPSCs-CM+LDH) exhibited reduced levels of ROS and content of MDA, increased SOD activity, improved migration ability, and decreased expression levels of p53 and p16 proteins in the cells after receiving UVB irradiation (P<0.01). Conclusion Pretreatment with DPSCs-CM combined with LDH nanomaterials can enhance the efficacy of DPSCs-CM alone in counteracting photoaging of HaCaT cells, providing a new direction for in vitro research on anti-skin photoaging. |
| Key words: Dental pulp stem cells-conditioned medium Layered double hydroxide HaCaT cells Photoaging |
