| 摘要: |
| 目的 探讨功能性电刺激（FES）联合头针对脑梗死大鼠前肢运动功能的影响，并基于沉默信息调节因子1（SIRT1）/过氧化物酶体增殖物激活受体γ共激活因子1α（PGC-1α）线粒体生物合成通路初步探讨其可能的作用机制。方法 将120只8周龄大鼠随机分为对照组、模型组、头针组、FES组、联合组（头针+FES），每组24只。通过改良线栓法构建脑梗死大鼠模型。采用Zea-Longa评分评估神经功能，前肢放置实验评估前肢的运动功能和神经恢复情况，2,3,5-氯化三苯基四氮唑（TTC）染色观察脑梗死体积，苏木精-伊红（HE）染色观察海马神经元结构及病理变化，酶联免疫吸附试验检测血清炎症因子水平，试剂盒检测海马组织氧化应激相关指标，透射电镜观察海马组织线粒体形态变化，蛋白质印迹法检测海马组织中SIRT1、PGC-1α蛋白水平。结果 与对照组相比，模型组中大鼠Zea-Longa评分升高，前肢踏步次数增多，脑梗死体积增大，海马组织损伤程度加重，白细胞介素6（IL-6）、肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）、丙二醛（MDA）水平升高，超氧化歧化酶（SOD）水平降低，线粒体损伤程度加重，SIRT1和PGC-1α蛋白表达水平降低，差异有统计学意义（P<0.05）。与模型组相比，头针组、FES组、联合组大鼠Zea-Longa评分降低，前肢踏步次数减少、脑梗死体积缩小，海马组织损伤程度减轻，IL-6、TNF-α、IL-1β、MDA水平降低，SOD水平升高，线粒体损伤程度减轻，SIRT1和PGC-1α蛋白表达水平升高，差异有统计学意义（P<0.05），且联合组上述指标变化较头针组和FES组显著（P<0.05）。结论 FES联合头针能改善脑梗死大鼠海马组织损伤，促进前肢运动功能的恢复，其机制可能与激活SIRT1/PGC-1α线粒体生物合成通路，降低炎症反应和氧化应激水平，改善线粒体功能有关。 |
| 关键词: 功能性电刺激 头针 SIRT1 PGC-1α 脑梗死 |
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| 基金项目:张家口市科技计划项目（2421052D） |
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| Exploring the Impact of Functional Electrical Stimulation Combined with Scalp Acupuncture on Forelimb Motor Function in Cerebral Infarction Rats Based on the SIRT1/PGC-1α Mitochondrial Biogenesis Pathway |
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The First Affiliated Hospital of Hebei North University
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| Abstract: |
| Objective To investigate the effects of functional electrical stimulation (FES) combined with scalp stimulation on forelimb motor function in rats with cerebral infarction, and to explore its possible mechanism of action based on the silencing information regulatory factor 1 (SIRT1)/peroxisome proliferator activated receptor gamma co activator factor 1 alpha (PGC-1 alpha) mitochondrial biosynthesis pathway. Method 120 8-week-old rats were randomly divided into a control group, a model group, a scalp acupuncture group, an FES group, and a combination group (scalp acupuncture+FES), with 24 rats in each group. Constructing a rat model of cerebral infarction by improving the suture method. The Zea Longa score was used to evaluate neurological function, and the forelimb placement experiment was conducted to evaluate the motor function and neurological recovery of the forelimbs. The infarct volume was observed by 2,3,5-triphenyltetrazolium chloride (TTC) staining, and the hippocampal neuron structure and pathological changes were observed by hematoxylin eosin (HE) staining. Enzyme linked immunosorbent assay was used to detect serum inflammatory factor levels, a reagent kit was used to detect oxidative stress-related indicators in hippocampal tissue, transmission electron microscopy was used to observe mitochondrial morphology changes in hippocampal tissue, and Western blotting was used to detect SIRT1 and PGC-1 α protein levels in hippocampal tissue. Results Compared with the control group, the Zea Longa score of rats in the model group increased, the number of forelimb strides increased, the volume of cerebral infarction increased, the degree of hippocampal tissue damage worsened, the levels of interleukin-6 (IL-6), tumor necrosis factor alpha (TNF - α), interleukin-1 β (IL-1 β), and malondialdehyde (MDA) increased, the level of superoxide dismutase (SOD) decreased, the degree of mitochondrial damage worsened, and the expression levels of SIRT1 and PGC-1 α proteins decreased, with statistical significance (P<0.05). Compared with the model group, the rats in the scalp acupuncture group, FES group, and combination group showed a decrease in Zea Longa scores, a reduction in the number of forelimb strides, a decrease in cerebral infarction volume, a reduction in hippocampal tissue damage, a decrease in IL-6, TNF - α, IL-1 β, MDA levels, an increase in SOD levels, a reduction in mitochondrial damage, and an increase in SIRT1 and PGC-1 α protein expression levels, with statistical significance (P<0.05). Moreover, the changes in the above indicators in the combination group were significantly higher than those in the scalp acupuncture group and FES group (P<0.05). Conclusion The combination of FES and scalp acupuncture can improve hippocampal tissue damage and promote the recovery of forelimb motor function in rats with cerebral infarction. The mechanism may be related to the activation of the SIRT1/PGC-1 α mitochondrial biosynthesis pathway, reducing inflammation and oxidative stress levels, and improving mitochondrial function. |
| Key words: Functional electrical stimulation Scalp acupuncture SIRT1 PGC-1α Cerebral infarctio |
