| 摘要: |
| ［摘要］ 目的 探讨调节性B细胞（Bregs）在糖尿病视网膜病变（DR）进展中的作用及机制。方法 选择8周龄雄性C57/BL6小鼠40只，将其随机分为DR组和对照组，各20只。DR组小鼠以30 mg/kg予腹膜内注射链脲佐菌素（STZ）构建DR模型；对照组小鼠予腹膜内注射相同体积的柠檬酸钠溶液。于28周龄时通过眼底照相检查、光学相干断层扫描（OCT）和眼底荧光素血管造影（FFA）观察小鼠眼底情况。收集小鼠脾脏和外周血标本，通过流式细胞术检测Bregs水平。招募2020年4月至2024年8月在广西壮族自治区人民医院收治的DR患者16例（病例组），其中增殖期DR（PDR）7例，非增殖期DR（NPDR）9例；于同期招募年龄匹配的健康体检者12名作为健康对照组，采用酶联免疫吸附试验（ELISA）检测血浆白细胞介素-10（IL-10）、肿瘤生长因子-β（TGF-β）和程序性死亡配体-1（PD-L1）水平，并分析其与疾病严重程度的相关性。结果 眼底彩照显示，DR组和对照组小鼠视网膜均未出现典型微血管瘤或出血渗出；OCT检查发现DR组小鼠视乳头前存在局部高反射灶；FFA检查结果显示，DR组小鼠视网膜微循环异常，具体表现为动静脉异常吻合支形成，符合DR的早期血管病理特征。流式细胞术检测结果显示，DR组小鼠脾脏和外周血Bregs水平较对照组降低，差异有统计学意义（P<0.05）。ELISA检测结果显示，病例组血浆IL-10、TGF-β和PD-L1水平均显著低于健康对照组（P<0.05）。PDR组血浆IL-10、PD-L1水平均显著低于NPDR组（P<0.05），两组血浆TGF-β水平比较差异无统计学意义（P>0.05）。结论 Bregs通过调控炎症反应参与DR的发生发展。 |
| 关键词: 糖尿病视网膜病变 调节性B细胞 白细胞介素-10 肿瘤生长因子-β 程序性死亡配体-1 |
| DOI：10.3969/j.issn.1674-3806.2026.02.12 |
| 分类号:R 774 |
| 基金项目:国家自然科学基金项目（编号：82560209）；广西自然科学基金项目（编号：2025GXNSFDA069013，2023GXNSFAA026127）；广西壮族自治区重大人才计划项目 |
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| Exploration on the role of regulatory B cell subsets in progression of diabetic retinopathy |
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KE Diyang1,2, CUI Ling2, WANG Hao2, DENG Wen2, CHEN Lifei2, XU Fan2, TANG Fen2, ZHONG Haibin2
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1.Guilin Medical University, Guilin 541001, China; 2.Department of Ophthalmology, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To explore the role and mechanism of regulatory B cells(Bregs) in progression of diabetic retinopathy(DR). Methods Forty male C57/BL6 mice aged 8 weeks were selected and randomly divided into control group and DR group, with 20 mice in each group. The mice in the DR group were intraperitoneally injected with streptozotocin(STZ) at a dose of 30 mg/kg to establish the DR model. The mice in the control group were intraperitoneally injected with the same volume of sodium citrate solution. At the age of 28 weeks, the fundi of the mice were observed by using fundus photography, optical coherence tomography(OCT) and fluorescein fundus angiography(FFA). The spleen and peripheral blood samples of these mice were collected and the levels of Bregs were detected by using flow cytometry. Sixteen patients with DR who were admitted to the People′s Hospital of Guangxi Zhuang Autonomous Region from April 2020 to August 2024 were recruited as the case group, among whom 7 patients had proliferative diabetic retinopathy(PDR) and 9 patients had non-proliferative diabetic retinopathy(NPDR), and 12 age-matched healthy individuals undergoing physical examination during the same period were recruited as the healthy control group. The plasma levels of interleukin-10(IL-10), tumor growth factor-β(TGF-β) and programmed death-ligand-1(PD-L1) were detected by using enzyme-linked immunosorbent assay(ELISA), and the correlations between these indicators and the severity of the disease were analyzed. Results Fundus color photography showed that no typical microangiomas or hemorrhagic exudates were observed in the retinas of the mice in both the DR group and the control group. OCT examination revealed the presence of localized hyperreflective foci in front of the optic papilla in the mice of the DR group. The FFA examination results showed that the microcirculation of the retinas in the mice of the DR group was abnormal, specifically manifested as the formation of abnormal arteriovenous anastomoses, which was consistent with the early vascular pathological characteristics of DR lesions. The detection results of flow cytometry showed that the levels of Bregs in the spleen and peripheral blood of the mice in the DR group were significantly lower than those in the control group(P<0.05). The detection results of ELISA showed that the plasma levels of IL-10, TGF-β, and PD-L1 in the case group were significantly lower than those in the healthy control group(P<0.05). The levels of plasma IL-10 and PD-L1 in the PDR group were significantly lower than those in the NPDR group(P<0.05), while there was no statistically significant difference in the plasma TGF-β level between the two groups(P>0.05). Conclusion Bregs are involved in the occurrence and progression of DR by regulating inflammatory responses. |
| Key words: Diabetic retinopathy(DR) Regulatory B cells(Bregs) Interleukin-10(IL-10) Tumor growth factor-β(TGF-β) Programmed death-ligand-1(PD-L1) |
