| 摘要: |
| ［摘要］ 目的 分析非小细胞肺癌（NSCLC）患者血清miR-515-5p、同源盒蛋白B13（HOXB13）水平及其与临床病理特征和预后的关系。方法 招募2019年7月至2021年7月佛山市第一人民医院收治的118例NSCLC患者作为研究组，另选取同期118名体检健康者作为对照组。采用实时荧光定量聚合酶链反应（RT-qPCR）法检测血清miR-515-5p、HOXB13 mRNA的相对表达量。患者出院后随访3年。根据随访期间患者生存情况将其分为预后不良组（51例）和预后良好组（67例）。采用Pearson相关分析miR-515-5p水平与HOXB13水平的相关性。采用Kaplan-Meier法绘制生存曲线，以log-rank检验进行组间比较。采用Cox回归分析NSCLC患者发生死亡的影响因素。结果 研究组miR-515-5p水平显著低于对照组（P<0.05），HOXB13水平显著高于对照组（P<0.05）。Pearson相关分析结果显示，NSCLC患者miR-515-5p水平与HOXB13水平呈负相关（r=-0.742，P<0.001）。miR-515-5p、HOXB13水平与临床分期、分化程度、淋巴结转移有关（P<0.05），与年龄、性别、病理类型、肿瘤直径无显著关联（P>0.05）。预后不良组血清miR-515-5p水平显著低于预后良好组（P<0.05），HOXB13水平显著高于预后良好组（P<0.05）。miR-515-5p高表达（≥0.50）患者的生存预后显著优于低表达（<0.50）患者（log-rank检验： χ2=36.627，P<0.001）。HOXB13低表达（<2.08）患者的生存预后显著优于高表达（≥2.08）患者（log-rank检验： χ2=12.755，P<0.001）。多因素Cox回归分析结果显示，高表达HOXB13、临床分期为Ⅲ～Ⅳ期、低分化程度、淋巴结转移是患者发生死亡的危险因素（P<0.05），高表达miR-515-5p是抑制患者发生死亡的保护因素（P<0.05）。结论 NSCLC患者血清miR-515-5p水平降低，HOXB13水平升高，二者与患者临床病理特征及预后有关。 |
| 关键词: miR-515-5p 同源盒蛋白B13 非小细胞肺癌 临床病理特征 预后 |
| DOI：10.3969/j.issn.1674-3806.2026.02.16 |
| 分类号:R 734.2 |
| 基金项目: |
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| The serum miR-515-5p and HOXB13 levels in non-small cell lung cancer patients and their associations with clinicopathological features and prognosis |
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WEI Xiaoqun, CHEN Yaofeng, LAI Qiting, LIU Jian, ZHANG Peifang
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Department of Respiratory and Critical Care Medicine, the First People′s Hospital of Foshan, Foshan 528000, China
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| Abstract: |
| ［Abstract］ Objective To analyze the serum microribonucleic acid(miR)-515-5p and homeobox protein B13(HOXB13) levels in non-small cell lung cancer(NSCLC) patients and their associations with clinicopathological features and prognosis. Methods A total of 118 patients with NSCLC who were admitted to the First People′s Hospital of Foshan from July 2019 to July 2021 were recruited as research group, and 118 healthy individuals who underwent physical examination during the same period were selected as control group. The levels of relative expressions of serum miR-515-5p and HOXB13 mRNA were detected by using real-time fluorescence quantitative polymerase chain reaction(RT-qPCR). The patients were followed up for 3 years after discharge from the hospital. According to the survival status of the patients during the follow-up period, they were divided into poor prognosis group(51 patients) and good prognosis group(67 patients). Pearson correlation was used to analyze the correlation between miR-515-5p level and HOXB13 level. The survival curve was plotted by using Kaplan-Meier method, and the inter-group comparison was conducted by using log-rank test. Cox regression was used to analyze the influencing factors of death in the NSCLC patients. Results The level of miR-515-5p in the research group was significantly lower than that in the control group(P<0.05), while the level of HOXB13 in the research group was significantly higher than that in the control group(P<0.05). The results of Pearson correlation analysis showed that the miR-515-5p level in the NSCLC patients was negatively correlated with the HOXB13 level(r=-0.742, P<0.001). The levels of miR-515-5p and HOXB13 in the NSCLC patients were related to TNM stage, degree of differentiation and lymph node metastasis(P<0.05), but were not significantly related to age, gender, pathological type and tumor diameter(P>0.05). The serum miR-515-5p level in the poor prognosis group was significantly lower than that in the good prognosis group(P<0.05), while the serum HOXB13 level in the poor prognosis group was significantly higher than that in the good prognosis group(P<0.05). The survival prognosis of the patients with high expression of miR-515-5p(≥0.50) was significantly better than that of the patients with low expression of miR-515-5p(<0.50)(log-rank test: χ2=36.627, P<0.001). The survival prognosis of the patients with low expression of HOXB13(<2.08) was significantly better than that of the patients with high expression of HOXB13(≥2.08)(log-rank test: χ2=12.755, P<0.001). The results of multivariate Cox regression analysis showed that high expression of HOXB13, TNM stages Ⅲ-Ⅳ, low degree of differentiation, and lymph node metastasis were risk factors for death in the patients(P<0.05), and high expression of miR-515-5p was a protective factor for inhibiting death in the patients(P<0.05). Conclusion Serum miR-515-5p level is decreased and serum HOXB13 level is increased in NSCLC patients. Both miR-515-5p and HOXB13 are related to the clinicopathological features and prognosis of the patients. |
| Key words: Microribonucleic acid(miR)-515-5p(miR-515-5p) Homeobox protein B13(HOXB13) Non-small cell lung cancer(NSCLC) Clinicopathological features Prognosis |
