| 摘要: |
| ［摘要］ 目的 阐明miR-182-5p在子宫内膜癌（EC）发展过程中的作用，并明确其与靶向基因的关系。方法 分析miR-182-5p在正常子宫内膜组织、细胞及EC组织、EC细胞（Ishikawa,HEC-1-A和KLE）中表达水平，并通过转染技术上调或下调miR-182-5p在不同细胞中的表达水平，采用MTT细胞增殖实验、Transwell侵袭实验和细胞划痕实验评估miR-182-5p对EC恶性生物学行为（包括细胞增殖能力、侵袭能力及迁移能力）的影响。利用生物信息学工具预测miR-182-5p的潜在靶基因，并通过逆转录实时定量聚合酶链反应（RT-qPCR）、Western blot实验检测靶基因在正常子宫内膜组织、EC组织及细胞中的表达。最后通过双荧光素酶报告基因实验验证miR-182-5p与其靶基因之间的特异性分子相互作用。结果 与正常子宫内膜组织、细胞相比，EC组织、细胞中miR-182-5p表达水平显著升高，转染miR-182-5p模拟物可显著促进EC细胞的侵袭性，而其抑制剂则产生相反效应。双荧光素酶报告基因实验证实MTSS1是miR-182-5p的直接靶基因，且二者表达水平呈负相关性。结论 miR-182-5p直接靶向并负向调控MTSS1基因，二者可能共同参与EC细胞恶性表型调节。 |
| 关键词: miR-182-5p MTSS1基因 子宫内膜癌 双荧光素酶报告基因实验 |
| DOI：10.3969/j.issn.1674-3806.2026.06.07 |
| 分类号: |
| 基金项目:北京市自然科学基金项目（编号：7162065） |
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| Targeted regulation of miR-182-5p on MTSS1 gene and its biological significance in endometrial cancer |
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TANG Shiqian1, ZHAO Yue2, DAI Yinmei1
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1.Department of Gynecology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University/Beijing Maternal and Child Health Care Hospital, Beijing 100026, China; 2.Medical Administration Division, Beijing Obstetrics and Gynecology Hospital, Capital Medical University/Beijing Maternal and Child Health Care Hospital, Beijing 100026, China
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| Abstract: |
| ［Abstract］ Objective To elucidate the role of microRNA-182-5p(miR-182-5p) in the progression of endometrial cancer(EC) and to clarify its relationship with targeted genes. Methods The expression levels of miR-182-5p in normal endometrial tissues and cells, as well as in EC tissues and EC cells(Ishikawa, HEC-1-A and KLE) were analyzed. The expression levels of miR-182-5p in different cells were upregulated or downregulated through transfection technology, and their effects on the malignant biological behaviors(including cell proliferative capacity, invasive capacity and migratory capacity) were evaluated by MTT cell proliferation assay, Transwell invasion assay and wound healing assay. Bioinformatics tools were used to predict the potential targeted genes of miR-182-5p, and the expressions of the targeted genes in normal endometrial tissues, EC tissues and cells were detected by using real-time quantitative reverse transcription polymerase chain reaction(RT-qPCR) and Western blot assay. Finally, the specific molecular interaction between miR-182-5p and its targeted genes was verified by using the dual-luciferase reporter gene assay. Results Compared with those in normal endometrial tissues and cells, the expression levels of miR-182-5p in EC tissues and cells were significantly increased. Transfection with miR-182-5p mimics could significantly enhance the invasion of EC cells, while their inhibitors had the opposite effect. The dual-luciferase reporter gene assay confirmed that metastasis suppressor 1(MTSS1) was a direct targeted gene of miR-182-5p, and the expression level of miR-182-5p was negatively correlated with that of MTSS1 gene. Conclusion miR-182-5p directly targets and negatively regulates MTSS1 gene, and the two indicators may jointly modulate the malignant phenotypes of EC cells. |
| Key words: MicroRNA-182-5p(miR-182-5p) Metastasis suppressor 1(MTSS1) gene Endometrial cancer(EC) Dual-luciferase reporter gene assay |
