| 摘要: |
| ［摘要］ 目的 观察隐丹参酮等中药单体对SZ95细胞中脂质合成的影响。方法 用尼罗河红荧光染色结合流式细胞术，以异维A酸为对照，观察隐丹参酮、丹参酮II-A、盐酸小檗碱、黄芩甙、苦参碱作用SZ95 细胞48 h后，1×104个尼罗河红染色的SZ95细胞中平均每个细胞的荧光强度变化。结果 1.25×10-7M丹参酮II-A使尼罗河红染色荧光强度下降最为明显（-34.3%，P<0.05），其次为1×10-4M黄芩甙（-30.1%，P<0.05）、1×10-7M异维A酸（-21.0%，P<0.05）、1.25×10-7M隐丹参酮（-16.2%）、1×10-6M盐酸小檗碱（-7.8%）也有抑制作用，但没有统计学差异。1×10-3M苦参碱则显示了促进脂质合成的作用（26.9%）。结论 丹参酮II-A、黄芩甙及异维A酸可能具有直接抑制皮脂腺细胞中脂质合成的作用。 |
| 关键词: 隐丹参酮 丹参酮II-A 皮脂腺细胞 痤疮 |
| DOI：10.3969/j.issn.1674-3806.2009.03.012 |
| 分类号:R 758.73 |
| 基金项目: |
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| Effects of cryptotanshinone and other traditional chinese medicines on the lipid synthesis of SZ95 sebocytes in vitro |
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LU Quan-xing, ZHANG Jin-yu
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Department of Dermatology, Zhaoyuan People′s Hospital, Zhaoyuan, Shandong 265400, China
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| Abstract: |
| ［Abstract］ Objective To investigate the effects of Chinese medicines cryptotanshinone, tanshinone II-A, berberine, baicalin, matrine on the lipid synthesis of SZ95 sebocytes compared with isotretinoin.Methods Lipid contents in the SZ95 sebocytes were labeled with a fluorescence dye(Nile red) and analyzed using flow cytometry.Results Lipid synthesis was inhibited by 1.25×10-7M tanshinone II-A（-34.2%）, 1×10-4M baicalin(-30.1%), 1×10-7M isotretinoin（-21.1%）， 1.25×10-7M Cryptotanshinone（-16.2%）and 1×10-5 M Berberine（-7.8%）also decreased lipogenesis but no statistical significance was found. Matrine increased lipogenesis（26.913%）.Conclusion Tanshinone II-A and baicalin may have a direct effect on lipid synthesis in sebocytes. |
| Key words: Cryptotanshinone Tanshinone II-A Sebocyte Acne |
