| 摘要: |
| ［摘要］ 目的 为选择方便、准确的方法定量检测产妇血清及乳汁HBV-DNA的含量，依据两项检测结果特别是乳汁中乙型肝炎病毒载量，指导母乳喂养。方法 选择HBsAg阳性的产妇232例，采用实时荧光定量聚合酶链反应法检测产妇血清及乳汁HBV-DNA的含量。结果 232例血清HBsAg阳性产妇血清HBV-DNA阳性177例，据血清HBV-DNA的含量，分≥105 copy/ml和<105 copy/ml两组,两组乳汁HBV-DNA阳性符合率分别为为54.4%和14.9%，两组比较差异有统计学意义（P<0.01）。血清HBV-DNA阴性者乳汁HBV-DNA均阴性，血清HBV-DNA阳性者乳汁HBV-DNA总阳性符合率为37.9%。结论 血清HBV-DNA定量检测阳性的产妇其乳汁中HBV-DNA检测阳性的占较大比例，且乳汁中HBV-DNA含量与血清病毒含量成正比。检测HBV感染产妇乳汁中HBV-DNA含量，对阻断母婴传播，为HBV感染产妇选择是否母乳喂养提供了指导依据。 |
| 关键词: 乙型肝炎病毒 母婴传播 荧光定量聚合酶链反应 母乳喂养 |
| DOI：10.3969/j.issn.1674-3806.2010.10.19 |
| 分类号:R 512.6 |
| 基金项目: |
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| HBV-DNA quantitative analysis and application in HBsAg-positive maternal serum and milk |
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YAN Shan-Huo, ZHUO Yong-guang
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Department of Clinical Laboratory, Qinzhou Maternal and Child Health Hospital,Guangxi 535000, China
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| Abstract: |
| ［Abstract］ Objective To choose a convenient, and accurate method for determining levels of HBV-DNA in the parturient maternal serum and the milk, and provide a breastfeeding guidance based on two test results, especially hepatitis B viral load in breast milk. Methods In two hundred and thirty-two HBsAg-positive parturient women, levels of HBV-DNA of maternal serum and the milk were detectcd using real-time fluorescence quantitative polymerase chain reaction method(FQ-PCR). Results In the 232 serum HBsAg-positive parturient women, there were 177 serum HBV-DNA positive women. According to the content of serum HBV-DNA these patients were divided into ≥ 105 copy/ml and <105 copy/ml groups, their milk HBV-DNA positive rate was 54.4% and 14.9% respectively, there was highly significant difference between two groups (P<0.01). In serum HBV-DNA negative women, HBV-DNA in milk were negative;in serum HBV-DNA positive women the milk HBV-DNA positive rate was 37.9%. Conclusion Serum HBV-DNA positive parturient women have a higher milk HBV-DNA-positive rate, and the milk HBV-DNA content is proportional to serum viral load. Detection of the HBV-DNA content of breast milk in HBV-infective parturient women has great signficance for interrupting mother to child transmission for HBV infection and providing a basis for guidance for the choice of breast-feeding mothers. |
| Key words: Hepatitis B virus(HBV) Mother to child transmission Fluorescence quantitative polymerase chain reaction Breastfeeding |
