| 摘要: |
| ［摘要］ 目的 探讨HLA-G基因3′端单核苷酸多态性与广西儿童哮喘发病的关联性。方法 采用基于基质辅助激光解吸电离-飞行时间质谱技术检测广西儿童支气管哮喘患者84例及正常对照儿童116例的HLA-G基因3′端单核苷酸多态性（rs1704和rs1063320）分型变化，并与HLA-DR基因型进行多元回归分析，寻找易感基因型及单体型。结果 rs1063320基因型构成比在哮喘组和对照组差异有统计学意义(P<0.05)；rs1704和rs1063320位点的14 bp缺失-G单体型能明显降低儿童哮喘的发病风险；14 bp缺失-G-HLA-DRB1*15XX单体型是儿童哮喘的保护性单体型，能更明显降低哮喘的发病风险，并使哮喘病人的血清IgE水平降低(P<0.05)。结论 HLA-G基因的3′单核苷酸多态性与广西儿童哮喘的发病相关。rs1704和rs1063320位点的14 bp缺失-G单体型是儿童哮喘的保护性单体型。 |
| 关键词: HLA-G 多态性 单核苷酸 哮喘 |
| DOI：10.3969/j.issn.1674-3806.2011.12.01 |
| 分类号:R 725.6 |
| 基金项目:广西自然科学基金资助项目（编号：桂科青0728037）；广西卫生厅科研课题（编号：Z2009167） |
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| Association of HLA-G 3′ untranslated regions polymorphism with children asthma |
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ZHEN Hong, XIE Qing-ling, TANG Xiao-yan, et al.
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Department of Pediatrics, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To investigate the correlation between single nucleotide polymorphisms in the 3′ untranslated regions of HLA-G gene and asthma occurrence of children.Methods Totally 84 children cases with asthma and 116 controls in Guangxi region were included into the study. The HLA-G gene single nucleotide poly-morphism (SNP) genotyping, rs1704 and rs1063320, was performed by using MassARRAY system (Sequenom) by means of matrix assisted laser desorption ionisation-time of flight mass spectrometry method (MALDI-TOF). And multiple regression analysis was performed to explore the asthma correlation.Results There was significant difference in the distribution of the genotypes of rs1063320 between cases and controls (P<0.05). The individuals with rs1704/rs1063320 14-bp deletion/G haplotype had decreased risk of asthma (odds ratio ［OR］=0.344, 95% confidence interval ［95%CI］=0.149～0.799,P<0.05), and the association with asthma of rs1704/rs1063320 14-bp deletion/G combined HLA-DRB1*15XX haplotype was more apparent with OR value of 0.198， 95%CI of 0.084～0.471(P<0.001), and with lower serum value of IgE in asthma cases (P<0.05).Conclusion HLA-G 3′ UTR SNPs has correlations with etiology of asthma. Having the haplotype of rs1704/rs1063320 14-bp deletion/G are protective from children asthma. |
| Key words: HLA-G gene Polymorphism Single nucleotide Asthma |
