| 摘要: |
| ［摘要］ 目的 研究体外培养急性髓性白血病树突状细胞（AML-DC），并利用该细胞联合细胞因子诱导脐血产生细胞毒性T淋巴细胞（CTL），观察CTL对急性白血病细胞的杀伤效应，探讨从AML-DC体外诱导产生CTL的可行性。方法 从急性髓性白血病细胞诱导AML-DC，联合细胞因子体外诱导脐血T细胞活化及增殖，流式细胞术检测培养前后的T淋巴细胞亚群变化，利用LDH试剂盒检测诱导后T细胞对相应急性白血病细胞的杀伤活性。结果 可从人AML细胞中诱导出AML-DC，脐血T细胞在体外经过诱导培养后可获得增殖，T淋巴细胞比例较培养前明显增高,其中在AML-DC诱导组中，CD3+ T淋巴细胞亚群比例达到（79.7±3.70）%，该T淋巴细胞对相应急性白血病细胞的杀伤效率最高达（48.35±12.75）%，与培养前及培养后无AML-DC刺激组相比，经过特异诱导培养的T细胞对相应白血病细胞杀伤作用大大加强（P<0.05）。结论 AML-DC联合细胞因子可以诱导活化脐血白血病细胞特异性细胞毒性T淋巴细胞(CTL)，该细胞能对相应白血病细胞产生特异性杀伤效应。 |
| 关键词: 脐血 T淋巴细胞 细胞因子 树突状细胞 急性髓性白血病 |
| DOI：10.3969/j.issn.1674-3806.2013.01.01 |
| 分类号:R 55 |
| 基金项目:国家自然科学基金资助项目（编号：81202236）；广州市医药卫生科技项目（编号：201102A213087） |
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| Studies on the specific anti-leukemia cell effect of cytotoxic T lymphocytes induced by acute myeloid leukemia-derived dendritic cells |
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WANG Cai-xia, ZHANG Yu-ping, WANG Shun-qing,et al.
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The Department of Hematology, Guangzhou First People’s Hospital Affiliated to Guangzhou Medical Cllege, Guangdong 510180,China
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| Abstract: |
| ［Abstract］ Objective To investigate the specific anti-leukemia cell effect of cytotoxic T lymphocytes(CTL) induced by acute myeloid leukemia-derived dendritic cells(AML-DC).Methods Acute myeloid leukemic cells were induced to differentiate into AML-DC.T cells from umbilical cord blood were activated with the cytokine combined with AML-DC. T cells were identified by flow cytometer(FCM). The anti-tumor activity of T cells was assayed with LDH method.Results Highly reactive anti-AML T cells were generated and expanded. The CD3+ T cells rate was (79.7±3.7)% in the group after stimulation by AML-DCs. These T cells caused specific lysis of (48.35±12.75)% target AML cells. And the difference was significant compared with T cells without stimulation of AML-DCs(P<0.05).Conclusion Leukemic cells of patients with acute myeloid leukemia could be differentiated to DC-like cells possessing the ability of stimulating anti-leukemic immune response. |
| Key words: Cord blood T cell Cytokine Dendritic cell Acute myeloid leukemia |
