| 摘要: |
| ［摘要］ 目的 探讨Caspase-9和Caspase-3在血管性痴呆大鼠海马CA1区的表达及意义。方法 将100只大鼠按随机数字法分为假手术组及模型组，每组又分为1、2、4、8和12周5个亚组（n=10只）。采用四血管阻断法制备血管性痴呆模型，应用Morris水迷宫试验检测大鼠的学习记忆能力，TUNEL检测细胞凋亡，免疫组化法检测Caspase-3及Caspase-9蛋白的表达。结果 （1）与假手术组比较，在相同时间点模型组大鼠海马CA1区凋亡细胞数明显增多，差异有统计学意义(P<0.05)；（2）与假手术组比较，模型组大鼠海马CA1区Caspase-9、Caspase-3阳性表达在1周开始增多，2周达到高峰，4周开始下降，到12周仍增多，差异有统计学意义(P<0.05)。结论 Caspase-9及Caspase-3共同参与了血管性痴呆大鼠海马CA1凋亡的调控。 |
| 关键词: 血管性痴呆 大鼠 凋亡 Caspase-9 Caspase-3 |
| DOI：10.3969/j.issn.1674-3806.2013.05.02 |
| 分类号:R 749.1+3 |
| 基金项目:唐山市科技局科技攻关计划应用项目(编号：121302110a) |
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| Expression and significance of Caspase-9 and Caspase-3 in CA1 area of hippocampus of vascular dementia rats |
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YUAN Min, LIU Bin, ZHENG Xiu- Qi
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First Department of Neurology, the Affiliated Hospital of Hebei United University，Tangshan 063000, China
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| Abstract: |
| ［Abstract］ Objective To observe the expression and significance of Caspase-9 and Caspase-3 in CA1 area of hippocampus of vascular dementia rats.Methods One hundred mice were randomly divided into sham operation group and vascular dementia model group. The groups were divided randomly into 1, 2, 4, 8 and 12 w groups (n=10). Vascular dementia rat models were established by blocking four vessels. Learning and memory abilities were detected by Morris water maze. Apoptotic cells were detected by TUNEL.The expression of Caspase-9 and Caspase-3 were detected by immunohistochemistry.Results (1)Compared to sham operation group, apoptotic cells were increased in CA1 aren of hippocampus,there were statistical significance at every time point of the model group(P<0.05).(2)Compared to sham operation group,the positive expression of Caspase-9 and Caspase-3 rose at 1 w, peaked at 2 w,at 4 w began to decline, to 12 w was higher still in vascular dementia model group, there were statistical significance(P<0.05).Conclusion Caspase-9 and Caspase-3 participated to regulate apoptosis together in hippocampal CA1 area of vascular dementia rats. |
| Key words: Vascular dementia Rat Apoptosis Caspase-9 Caspase-3 |
