| 摘要: |
| ［摘要］ 目的 观察来氟米特活性代谢产物A771726对脂多糖诱导下HaCaT细胞增殖和凋亡的影响。方法 采用MTT法检测细胞增殖水平的变化；流式细胞仪检测细胞凋亡；倒置相差显微镜下观察细胞形态。结果 A771726对脂多糖诱导下HaCaT细胞的增殖有明显的抑制作用，具有时间-浓度依赖性； A771726能促进细胞凋亡，凋亡率随浓度的增加而增加；倒置显微镜下观察，脂多糖组细胞呈致密单层，随着A771726浓度升高，细胞体积变小、细胞间隙变大、细胞内空泡增多。结论 来氟米特可以抑制脂多糖诱导下HaCaT细胞增殖，促进其凋亡，这可能是来氟米特治疗银屑病的作用机制之一。 |
| 关键词: 来氟米特 角质形成细胞 银屑病 凋亡 |
| DOI：10.3969/j.issn.1674-3806.2014.04.09 |
| 分类号:R 751 |
| 基金项目:南宁市科学研究与技术开发计划项目（编号：2007011408C） |
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| Effects of leflunomide on the proliferation and apoptosis of the lipopolysaccharide-induced HaCaT cells |
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CHENG Qiu-mei, PAN Yan-bin, TAN Mei-le,et al.
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Department of Dermatological, the Second People′s Hospital Nanning, Guangxi 530031，China
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| Abstract: |
| ［Abstract］ Objective To observe the proliferation and apoptosis of the lipopolysaccharide (LPS)-induced HaCaT cells in the presence of the active leflunomide metabolite A771726.Methods MTT was used to detect the level of cell proliferation; flow cytometry (FCM) was used to detect apoptosis of HaCaT cells; inverted phase contrast microscope (IPCM) was used to observe morphologic changes.Results The A771726 significantly inhibited the proliferation of LPS-induced HaCaT cells, the inhibition effect was concentration-and-time dependent; the A771726 promoted cell apoptosis and the apoptosis rate increased with its concentration, the LPS group showed dense monolayer cells under an inverted microscope, as the concentration of the A771726 increased, the volume of the cells got smaller and intercellular space got larger, and the more vacuoles was observed in the cells.Conclusion Leflunomide can inhibit the proliferation of the LPS induced HaCaT cells and expedite their apoptosis, which may be one of the mechanisms of leflunomide in the treatment of psoriasis. |
| Key words: Leflunomide HaCaT Psoriasis Apoptosis |
