| 摘要: |
| ［摘要］ 目的 观察实时荧光PCR检测耐甲氧西林金黄色葡萄球菌（MRSA）对减少儿科ICU病区医院感染的效果。方法 选取2011-07～2012-06实时荧光PCR法检测的儿科ICU病区标本856份,其结果与分离培养药敏试验法的检测结果进行比较；另与2009-07～2010-06 MRSA医院感染率进行比较。结果 实时荧光PCR法与分离培养药敏试验法对金葡菌检测的一致率为100%，均检测出33株金葡菌阳性株。实时荧光PCR法与分离培养药敏试验法检测MRSA的符合率为93.9%。分离培养药敏试验法检测为阴性而实时荧光PCR法检测为阳性的2株待检菌的测序结果显示为阳性。利用实时荧光PCR法检测MRSA后，医院感染率从36.4%下降到16.7%。结论 实时荧光PCR法能更及时、更准确发现MRSA,从而早隔离、早治疗，减少MRSA医院感染。 |
| 关键词: 耐甲氧西林金黄色葡萄球菌 实时荧光PCR 医院感染 儿科ICU病区 |
| DOI：10.3969/j.issn.1674-3806.2015.06.14 |
| 分类号:R 378.1+1 |
| 基金项目: |
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| Reduction of nosocomial infection of MRSA in pediatric intensive care unit by real-time fluorescence PCR |
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DAI Ying, WANG Qi-wei, HE Shu, et al.
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Department of Pediatrics, Huaihe Hospital of Henan University, Kaifeng 475003, China
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| Abstract: |
| ［Abstract］ Objective To reduce nosocomial infection of meticillin-resistant staphylococcus aureus(MRSA) in pediatric intensive care unit(PICU) by real-time fluorescence PCR.Methods From July 2011 to June 2012, 856 specimens in PICU were detected for MRSA by using real-time fluorescence PCR and isolation-culture-K-B paper disk diffusion method. The nosocomial infection rate during the period from July 2009 to June 2010 was compared with that from July 2011 to June 2012.Results Thirty-three strains of staphylococcus aureus were identified by culture and real-time fluorescence PCR. Of the 33 clinical isolated staphylococcus aureus, 12 isolates were defined as MRSA by K-B paper disk diffusion method and 14 isolates were defined as MRSA by real-time fluorescence PCR. The two isolates which were defined as positive by real-time fluorescence PCR and negative by K-B paper disk diffusion method were proved to be MRSA positive by sequencing method. Nosocomial infection rates reduced from 36.4%, 37.5% to 16.7%.Conclusion The real-time fluorescence PCR can identify MRSA more rapidly and accurately and help reduce the rate of nosocomial infection of MRSA in PICU. |
| Key words: Methicillin resistant staphylococcus aureus Real-time fluorescence PCR Nosocomial infection Pediatric intensive care unit(PICU) |
