| 摘要: |
| ［摘要］ 多重置换扩增（MDA）技术作为一种全新的全基因组扩增技术，为单细胞的后续检测提供了足够和稳定的DNA，在地中海贫血植入前遗传学诊断的实验研究和临床应用中取得了较大进展。该文就MDA的原理、技术特点及其在地中海贫血植入前遗传学诊断中的应用研究进展进行综述。 |
| 关键词: 多重置换扩增 植入前遗传学诊断 地中海贫血 |
| DOI：10.3969/j.issn.1674-3806.2015.10.35 |
| 分类号:R 394 |
| 基金项目:广西卫计委科研课题（编号：Z2013343）；广西自然科学基金资助项目（编号：2011GXNSFA018302） |
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| Multiple displacement amplification and its application in preimplantation genetic diagnosis of thalassemia |
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WANG Shi-kai, HUANG Li, HE Bing
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Reproductive and Genetic Center, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
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| Abstract: |
| ［Abstract］ As an entirely new genome amplification technology to provide sufficient and stable DNA for subsequent detection of single cell, multiple displacement amplification(MDA) has acquired a great progress in experimental research and clinical application in preimplantation genetic diagnosis of thalassemia. In this review, we introduce the principles of MDA, technical characteristics and research progress in preimplantation genetic diagnosis of thalassemia. |
| Key words: Multiple displacement amplification （MDA） Preimplantation genetic diagnosis（PGD） Thalassemia |
