| 摘要: |
| ［摘要］ 目的 探究应用丙戊酸钠（valproate,VPA）对大鼠癫痫脑损伤时海马胶质原纤维蛋白（glial fibril protein,GFAP）表达的影响。方法 选择成年健康SD雄性大鼠80只，以随机数字表法分为A组、B组、C组以及D组各20只。A组不作任何干预措施；B组只进行单纯的VPA洗胃；C组为癫痫模型，不进行任何治疗；D组为癫痫模型，应用VPA治疗。对比各组大鼠海马CA1、CA2区GFAP阳性细胞数、平均截面积、平均灰度值、累积光密度以及平均光密度。结果 在海马CA1、CA2区GFAP阳性细胞数C、D组显著高于A、B组，并且D组低于C组。海马区CA1、CA2区中平均截面积、累积光密度以及平均光密度C、D组高于A、B组，并且C组高于B组；而C、D组平均灰度值低于A、B组，并且C组低于B组，差异均有统计学意义（P均<0.05）。结论 VPA具有显著抑制癫痫大鼠模型中GFAP表达的效果，同时减少脑损伤程度，安全程度较高。 |
| 关键词: 癫痫 脑损伤 丙戊酸钠 海马胶质原纤维蛋白 |
| DOI：10.3969/j.issn.1674-3806.2016.06.02 |
| 分类号:R 742.1 |
| 基金项目:深圳市宝安区科技计划社会公益项目(编号：2013037) |
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| Effect of sodium valproate on the expressions of GFAP in rats with epileptic brain injury |
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LU Wang-ke, GAO Ran
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Department of Emergency, the People′s Hospital of Baoan District, Shenzhen City, Guangdong 518101, China
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| Abstract: |
| ［Abstract］ Objective To explore the effect of sodium valproate(VPA) on the expressions of glial fibril protein(GFAP) in hippocampus of rats with epiltic brain injury.Methods Eighty adult male SD rats were randomly divided into group A, group B, group C and group D, with 20 cases in each group. No intervention was given to group A; group B was only treated with VPA gastric lavage; Epileptic rat′s models were established in both group C and group D, with the treament of valproate in group D and without any intervention in group C. The number of CA1 positive cells in CA2 and GFAP regions, the average cross-sectional area, the average gray value, the accumulated optical density, and the average optical density were compared among the four groups.Results The numbers of GFAP positive cells in CA2 and CA were significantly higher in group C and group D than those in group A and group B, and the number of GFAP cells in group D was significantly lower than that in group C. The mean cross-sectional area of CA1 or CA2, was significantly higher in group C or group D than that in group A or group B, and the mean area of CA1 or CA2 in group C was higher than that in group B. The average gray values were significantly lower in group C and group D than group A and group B, and the one in group C was lower than that in group B.Conclusion Valproate can significantly inhibit the expression of GFAP in rat model of epilepsy, and reduce the degree of brain damage. |
| Key words: Epilepsy Brain injury Sodium valproate Hippocampal glial fibrillary protein |
