| 摘要: |
| ［摘要］ 目的 探讨EGCG抑制皮肤鳞状细胞癌SCL-1细胞的增殖作用及其机制。方法 取对数生长期的SCL-1细胞，分别加入20 μm、60 μm和100 μm的EGCG作用24 h后，四甲基偶氮唑盐（MTT）法检测相对细胞增殖率，免疫印迹法检测增殖细胞核抗原（PCNA）、PTEN和磷酸化蛋白激酶B（p-AKT）蛋白表达。结果 20 μm、60 μm及100 μm的EGCG作用SCL-1细胞24 h后，相对细胞增殖率分别为(84.25±3.3)%、(71.34±2.3)%和(60.33±4.5)%，各浓度组与空白对照组［(100.01±2.7)%］比较，差异均有统计学意义（P均<0.05）。与空白对照组相比，不同浓度EGCG作用细胞PCNA蛋白表达亦显著降低（P均<0.05）。检测PTEN-PI3K/AKT信号途径蛋白，与空白对照组比较，EGCG作用可使细胞PTEN表达显著升高（P均<0.05），而p-AKT表达显著降低（P均<0.05）。结论 EGCG作用能够抑制皮肤鳞状细胞癌SCL-1细胞的增殖作用，其作用可能通过PTEN-PI3K/AKT信号途径介导。 |
| 关键词: 皮肤鳞状细胞癌 表没食子儿茶素没食子酸酯 细胞增殖 PTEN-PI3K/AKT |
| DOI：10.3969/j.issn.1674-3806.2016.07.08 |
| 分类号:R 739.5 |
| 基金项目:广西医药卫生科研课题（编号：Z2013377） |
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| Proliferation inhibition by EGCG on SCL-1 cells through PTEN-PI3K-AKT signaling and the regulation mechanisms |
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LI Li-li, CHEN Xian-feng, HUANG Qi-tao, et al.
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Department of Dermatology, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To investigate the effect of (-)-epigallocatechin-3-gallate(EGCG) on SCL-1 cell proliferation and the regulation mechanisms.Methods SCL-1 cells were treated with 20 μm, 60 μm or 100 μm EGCG. The proliferation was detected by MTT when cells were treated with EGCG for 24 h. Western blot was used to detect the expressions of PCNA, PTEN and p-AKT.Results In the EGCG-treated groups, the cell proliferation rates of SCL-1 cells were (84.25±3.3)%, (71.34±2.3)% and (60.33±4.5)%, when the cells were treated with EGCG at 20, 60 and 100 μm respectively. There were significant differences between the EGCG-treated groups and the control group［(100.01±2.7)%］(P<0.05). The expression of PCNA was significantly decreased when the cells were treated with EGCG, moreover, it significantly increased the expression of PTEN (P<0.05), and obviously decreased the p-AKT levels(P<0.05).Conclusion EGCG inhibits the proliferation of SCL-1 cells through PTEN-PI3K-AKT signaling pathway. |
| Key words: Squamous cell carcinoma (-)-epigallocatechin-3-gallate Cell proliferation PTEN-PI3K/AKT |
